Cells digitonin. Medical search. Frequent questions

Thus, we conclude that ADF/cofilin activity is not generally required for induction or progression of apoptosis in mammalian cells. (nature.com)
In summary, our work demonstrates that in mammalian cells ADF/cofilin activity is not generally required for efficient induction of apoptosis. (nature.com)
Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. (elifesciences.org)

Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that translocated by a yet unknown mechanism to mitochondria during cell death. (nature.com)
Importantly, the mitochondrial association of n-cofilin and ADF had no impact on apoptosis signaling because ablation of either n-cofilin, ADF, or both proteins together did not change cell death progression. (nature.com)
Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
Torres-Quesada O, Doerrier C, Strich S, Gnaiger E, Stefan E (2022) Physiological cell culture media tune mitochondrial bioenergetics and drug sensitivity in cancer cell models. (bioblast.at)
Stadlmann S, Renner K, Pollheimer J, Moser PL, Zeimet AG, Offner FA, Gnaiger E (2006) Preserved coupling of oxidative phosphorylation but decreased mitochondrial respiratory capacity in IL-1ß treated human peritoneal mesothelial cells. (mitophysiology.org)
Bmal1+/+ and Bmal1-/- BMDMs were incubated in an isosmotic, ADP-supplemented mitochondrial assay solution and permeabilized with digitonin. (bariatricnews.net)

These proteases are responsible for the dismantling of cells during apoptosis. (nature.com)
To test the relevance of ADF/cofilin activity for apoptotic processes, we here employed a genetically well-defined system of mouse embryonic fibroblasts (MEFs) devoid of any ADF/cofilin activity and challenged these cells with well-characterized apoptosis inducers. (nature.com)
Ectopic Mcl-1 expression attenuates Bak activation and apoptosis by ABT-737 + roscovitine, whereas cells overexpressing Bcl-2 or Bcl-xL remain fully sensitive. (aacrjournals.org)
HA14-1) that disable Bcl-2, resulting in induction of apoptosis in leukemia cell lines ( 8 ). (aacrjournals.org)
that critically influences cell proliferation, differentiation and apoptosis ( Miele and Osborne, 1999 ). (elifesciences.org)
In bax − / − bak − / − cells, introduction of Bak mutants selectively targeted to either mitochondria or the ER can induce apoptosis. (rupress.org)
Eberhart K, Renner K, Ritter I, Kastenberger M, Singer K, Hellerbrand C, Kreutz M, Kofler R, Oefner PJ (2009) Low doses of 2-deoxy-glucose sensitize acute lymphoblastic leukemia cells to glucocorticoid-induced apoptosis. (mitophysiology.org)
In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
The signaling pathways further induced apoptosis of U937 cells through the mitochondrion-mediated death pathway. (ecolowood.com)
In the present study, we observed that inactivation of the ERK1/2 signaling pathway using ABT-737 dramatically increased the expression of pro-apoptotic protein Bim via transcriptional and/or posttranslational regulation, in a cell type-dependent manner, inducing mitochondria-mediated apoptosis of human oral cancer cells. (oncotarget.com)
Apoptosis is a natural barrier that prevents cancer cells from surviving and disseminating to other tissues. (oncotarget.com)

This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)
Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibres from small biopsies of muscle, and isolated mitochondria. (bioblast.at)
Living cells, permeabilized cells and tissue, or isolated mitochondria? (bioblast.at)
Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. (bioblast.at)
Sorting in plant cells is complex, as mitochondria and chloroplasts or plastids coexist in every cell. (silverchair.com)
Digitonin has been used in a study to assess a procedure for isolating mitochondria from ascites tumor cells. (yunbangpharm.com)
They also found that, in the mitochondria (the cells energy powerhouse), the pathways by which glucose was further broken down to produce energy were very different in macrophages without a clock. (bariatricnews.net)

Digitonin is a steroidal saponin (saraponin) obtained from the foxglove plant Digitalis purpurea. (wikipedia.org)
1. Prepare dead cells by 10 minutes incubation in 0.1% saponin or 0.1-0.5% digitonin or by 30 minutes incubation in 70% ethanol. (dojindo.com)

2. Primary Antibody와 pAG-MNase Fusion Enzyme이 핵으로 유입되는 것을 촉진시키기 위해 Digitonin으로 Cell Membrane Permeabilization. (seoulin.co.kr)

Fig. 2 Cell staining with Double Staining Kit HeLa cell, incubated with assay solution for 15 minutes. (dojindo.com)

This kit contains Calcein-AM and Propidium Iodide (PI) solutions, which stain viable and dead cells, respectively (Fig. 1). (dojindo.com)
The radiosensitizing effect of doranidazole was determined using colony formation and propidium iodide (PI) assays to measure cell growth inhibition and the cell killing effect of human colorectal cancer cell lines exposed to high doses of γ-ray irradiation under hypoxic conditions in vitro . (biomedcentral.com)
MTT 2 propidium iodide digitonin U0126 (MEK1 and MEK2 inhibitor) SB202190 (p38 MAPK inhibitor) and anti-β-actin antibody were from Sigma and annexin V-FITC/propidium iodide circulation cytometry assay kit and rhodamine-123 were purchased from Invitrogen. (ecolowood.com)

Uptake of digoxin was found to be similar among rat periportal (PP) and perivenous (PV) hepatocytes isolated by the digitonin-collagenase method. (elsevierpure.com)

indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
Cell proliferation evaluated by clonogenic survival curves was significantly inhibited by 5 mmol/L doranidazole, particularly at doses ranging from 10 to 30 Gy of irradiation. (biomedcentral.com)
Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)

We demonstrated leukosomes biocompatibility, endothelium adhesion capability, and tumor target in three-dimensional (3D) settings using CRC cell lines. (frontiersin.org)
Therefore, there is substantial interest in developing chemotherapeutic drugs that directly target pro-survival Bcl-2 proteins by mimicking the BH3 domain and unleashing pro-apoptotic molecules in tumor cells. (oncotarget.com)
Among the numerous available small molecule BH3 mimetics, ABT-737, a potent small molecule that binds to Bcl-2/Bcl-xL with high affinity, has anti-tumor activity in a wide variety of cancer cells. (oncotarget.com)

Need to perform ATAC-Seq on fresh or frozen cells or tissues? (activemotif.com)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
3 , 4 The labeling of antibodies with the fluorescent tag, fluorescein, and the detection of their respective antigens in cells and tissues was pioneered by Albert Hewett Coons and others in 1941 5 and kickstarted the immunostaining revolution. (technologynetworks.com)
With high GM values (2400 to 13300), the LipiBright SMCy3.5 and SMCy5.5 probes have been confirmed to work with two photon microscopy to work in multiple models including cells and tissues 1 . (cytoskeleton.com)
It had been recommended that bikunin and tissues aspect pathway inhibitor-2 exerted their biology actions through a cell surface area receptor-mediated procedure (3 4 Furthermore tissue aspect pathway inhibitor-2 elicits pro-apoptotic signaling pathway in the individual fibrosarcoma cell series (8). (ecolowood.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

J Cell Biol (2002) 159 (3): 403-410. (rupress.org)
J Cell Biol (2003) 162 (1): 59-69. (rupress.org)
J Cell Biol (2000) 148 (6): 1213-1222. (silverchair.com)

Since optimal staining conditions differ from cell line to cell line, we recommend that a suitable concentration of PI and Calcein- AM be individually determined. (dojindo.com)
2. Stain dead cells with 0.1-10 μM PI solution to find a PI concentration that stains the nucleus only, not the cytosol. (dojindo.com)
3. Stain dead cells with 0.1-10 μM Calcein-AM solution to find a Calcein-AM concentration that does not stain the cytosol. (dojindo.com)
Optimum digitonin concentration test (SUIT-010). (bioblast.at)
Solubilization of the receptor was accomplished with digitonin which was added in increments (over 10 min) to a final concentration of 0.8% (mass/vol). (mpg.de)

For this purpose, a cellular model of senescent myocardial cells was set up and evaluated using colorimetric, fluorimetric, and immunometric techniques. (hindawi.com)
Relevant cellular senescence markers, such as X-gal staining, cell cycle regulator levels, and the percentage of cell cycle-arrested cells, were found to be reduced in the presence of naringenin. (hindawi.com)
Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, together, the data have prompted us to investigate the effects of Nar in a cellular model of aged myocardial cells. (hindawi.com)
Various cellular senescence hallmarks (the percentage of X-gal staining cells, the mRNA levels of the p16 and p21 cell cycle regulators, and the percentage of cell cycle-arrested cells) were investigated. (hindawi.com)
Use of Fluorescent Dyes in the Determination of Adherence of Human Leucocytes to Endothelial Cells and the Effects of Fluorochromes on Cellular Function. (dojindo.com)
Although hypoxic or ischemia tolerance in AGS involves physiological adaptations, little is known about the critical cellular mechanisms underlying intrinsic AGS cell resilience to metabolic stress. (elifesciences.org)
The exact function of aSyn remains unclear, but it is known to be associated with vesicles and membranes, and to have an impact on important cellular functions such as intracellular trafficking and protein degradation systems, leading to cellular pathologies that can be readily studied in cell-based models. (biomedcentral.com)
Recently, novel potent and cell active HDAC11 inhibitors have been developed, which can be used for cellular assay validation (FT895 and compound 14). (openlabnotebooks.org)
To guage the particular power from the probe CP1-KS in biological discipline, this particular probe had been used as a possible extracellular phosphorescent probe to monitor the actual efflux of K+ via cells (E coli, W. Subtilis 168, Hela and also MCF-7 cellular material) under various excitement which include lysozyme, nigericin, digitonin, as well as ATP. (ubiquitininhibitors.com)

Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. (go.jp)
The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme. (go.jp)

Wide-field fluorescent imaging of live Swiss3T3 labeled with LipiBright™ SMCy3.5 (Pink), a live cell actin cytoskeleton stain (Green, SiR-Actin), and a live cell DNA probe (Blue, SPY505-DNA). (cytoskeleton.com)

As such, it has several potential membrane-related applications in biochemistry, including solubilizing membrane proteins, precipitating cholesterol, and permeabilizing cell membranes. (wikipedia.org)

Schier, A. F. Single-cell biology: beyond the sum of its parts. (nature.com)
Her research has involved using molecular, cell and computational biology tools and techniques to understand the pathological mechanisms underlying neurological disorders. (technologynetworks.com)

Calcein-AM, an acetoxymethyl ester of calcein, is highly lipophilic and cell membrane permeable. (dojindo.com)

Because soybean Kunitz-type trypsin inhibitor has been found to induce apoptotic death of human being leukemia Jurkat cells (17) anti-leukemia activity of B. multicinctus protease inhibitor-like proteins is definitely therefore examined. (ecolowood.com)

CUT&RUN and ChIP assays were performed with HCT 116 cells and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 or Rabbit (DA1E) mAb IgG XP ® Isotype Control (CUT&RUN) #66362, using this CUT&RUN Assay Kit (left panel) or the SimpleChIP ® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005 (right panel). (cellsignal.com)
Fluorescence staining and cell migration assays were also used to assess the radiosensitizing effect. (biomedcentral.com)
Target engagement assays allow us to test if potential inhibitors enter the cell and interact with a target protein. (openlabnotebooks.org)

Here we show that in cells depleted of the SRP protein, Ffh or the translocon component SecE, the ribosomal targeting pathway is blocked downstream and unprecedented, membrane-bound FtsY-ribosomal complexes are captured. (rupress.org)
On the other hand, PI, a nuclei staining dye, cannot pass through a viable cell membrane. (dojindo.com)
It reaches the nucleus by passing through disordered areas of dead cell membrane and intercalates with the DNA double helix of the cell to emit red fluorescence (excitation: 535 nm, emmision: 617 nm). (dojindo.com)

Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
Examining the genetic code of these resilient cells revealed that Arctic ground squirrels may have a variant form of a protein called ATP5G1. (elifesciences.org)
These findings provide the first evidence of a protein variant that is responsible for protecting cells during the metabolic stress conditions caused by hibernation. (elifesciences.org)
Using B cells from homozygous HAQ carriers, we found, surprisingly, that HAQ/HAQ carriers express extremely low MPYS protein and have a decreased TMEM173 transcript. (aai.org)
The mHAQ mouse has decreased MPYS protein in B cells, T cells, Ly6C hi monocytes, bone marrow-derived dendritic cells, and lung tissue. (aai.org)
NPCs mediate the exchange of thousands of molecules per second between the nucleus and the cytoplasm of eukaryotic cells 1 with typical protein transit times of ~10 ms. 2-4 The human NPC scaffold has an outer diameter of ~110 nm, and a minimum diameter of ~50 nm, 5,6 although recent reports of ~60 nm 'dilated' pores are potentially more physiologically relevant. (researchsquare.com)
29937980 ). In the assay you are transfecting cells with protein fused to Nanloluc luciferease, treat with inhibitors and measure bioluminescent signal after heat denaturation. (openlabnotebooks.org)
Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. (biomedcentral.com)
The protein kinase is found in the cytosol of pea mesophyll cells or in wheat germ extract. (silverchair.com)
With this study human being leukemia U937 cells were treated with B. multicinctus protease inhibitor like protein-1 (PILP-1). (ecolowood.com)
Collectively our data elucidate a novel ADAM17/Lyn/Akt signaling pathway in keeping the viability of leukemia cells and suggest a strategy in improving leukemia therapy through suppression of ADAM17 protein expression. (ecolowood.com)

The m3 muscarinic acetylcholine receptor from rat heterologously produced in insect cells after infection with a recombinant baculovirus has an apparent molecular mass of approximately 75 kDa. (mpg.de)
Polyclonal antibodies raised against a carboxy-terminal nonapeptide that is unique to the m3 subtype can detect the receptors produced in the insect cells by Western blot and can also immunoprecipitate solubilized receptor. (mpg.de)
Immunofluorescence microscopy as well as electron microscopy revealed that the receptor was located intracellularly, visualized as a ring around the nucleus of the infected insect cells. (mpg.de)
From the behaviour of the solubilized receptor during DEAE-Sephacel chromatography and lectin-affinity chromatography it can be deduced that the receptor produced in insect cells is heterogeneously glycosylated in the producing insect cells. (mpg.de)
Right after experience with diverse computer pretreatments, the actual ensuing Killer cell immunoglobulin-like receptor range contains highest quantity of data regarding monosaccharide items throughout Atractylodes polysaccharides. (ubiquitininhibitors.com)

In the present study, we examined the radiosensitizing effect of doranidazole on human colorectal cancer cells by high-dose irradiation in vitro. (biomedcentral.com)
Combining in vitro studies with studies in cell models, we found that this mutation reduces aSyn aggregation and increases proteasome activity, altering normal proteostasis. (biomedcentral.com)
The compounds work in our in vitro assay (IC50 in nM range), but I do not have any proof that they really work in cells. (openlabnotebooks.org)

Indeed, the principal adipocytes isolated from obese mice could induce antigen-specific Compact disc4+ T cell activation (58). (exposed-skin-care.net)

Genome browser tracks of sorted CD4+ cells from human blood samples compared with K562 cells and ENCODE CD4+ data. (activemotif.com)
Cell Culture Individual severe myelogenous leukemia U937 cells and individual chronic myelogenous leukemia K562 cells extracted from ATCC (Manassas VA) had been grown up in RPMI 1640 moderate supplemented with 10% fetal leg serum (Invitrogen) 2 mm l-glutamine penicillin (100 systems/ml)/streptomycin (100 μg/ml) and 1% sodium pyruvate incubated at. (ecolowood.com)

Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
Knockdown of autophagy-related gene 5 inhibited basal autophagy and diminished oxidative stress-induced autophagy and cell death. (biomedcentral.com)
Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)

Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
Multiplexed quantification of proteins and transcripts in single cells. (nature.com)

The Fixed Cell ATAC-Seq Kit from Active Motif provides the reagents necessary to produce 16 unique sequencing-ready Illumina®-compatible ATAC-Seq libraries from 50,000 - 100,000 formaldehyde-fixed cells per reaction. (activemotif.com)
The Fixed Cell ATAC-Seq kit contains the necessary reagents and enzymes to perform 16 ATAC-Seq reactions on formaldehyde-fixed cell samples. (activemotif.com)
Cell lifestyle items were purchased from Invitrogen Unless buy 435-97-2 specific all the reagents were of analytical quality in any other case. (ecolowood.com)

The researchers found that macrophages without a body clock took up far more glucose and broke it down more quickly than normal cells. (bariatricnews.net)

In the Fixed Cell ATAC-Seq assay, intact nuclei from formaldehyde-fixed cell samples are treated with a hyperactive Tn5 transposase mutant. (activemotif.com)
Fluorescence is recovered from NPCs within the largely flat bottom NE of the nuclei of permeabilized U-2 OS cells ( NCB Figure 1a,b ) using a narrow-field epifluorescence scheme (constrained illumination field). (researchsquare.com)

Because aging has been demonstrated to be directly related to the occurrence of cardiac disorders, in the present study, the ability of naringenin to prevent cardiac cell senescence was investigated. (hindawi.com)
To the best of our knowledge, the effect of Nar in cardiac cell senescence has not yet been studied. (hindawi.com)

Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
But, it is unclear how their cells are able to endure this metabolic stress. (elifesciences.org)
Cancer cells produce greater levels of ROS than normal cells do because of increased metabolic stresses. (biomedcentral.com)

Here, we show that Mcl-1 down-regulation by the cyclin-dependent kinase (CDK) inhibitor roscovitine or Mcl-1-shRNA dramatically increases ABT-737 lethality in human leukemia cells. (aacrjournals.org)

This paper investigates the effects of a new radiosensitizer, doranidazole, and enhancing irradiation on colorectal cancer cells. (biomedcentral.com)
The radiosensitizing effect of doranidazole on colorectal cancer cells occurs in a time- and dose-dependent manner. (biomedcentral.com)
However, excessive production of ROS kills cancer cells. (biomedcentral.com)
It is not fully understood how cancer cells grow rapidly and survive in the presence of high ROS levels. (biomedcentral.com)
It has also been used in a study to investigate its effect on the cytotoxicity of plant secondary metabolites in cancer cells. (yunbangpharm.com)
However, cancer cells use various strategies to avoid apoptotic cell death by modulating multiple key regulators of the apoptotic signaling pathway. (oncotarget.com)

Fig. 6: Multiplexed CRISPR perturbations with ASAP-seq in primary human T cells. (nature.com)
Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)

low dosages of GO promoted cell growth, while high doses induced cell death. (biomedcentral.com)

Cellstain-Double Staining Kit is utilized for simultaneous fluorescence staining of viable and dead cells. (dojindo.com)
Although Calcein-AM itself is not a fluorescent molecule, the calcein generated from Calcein- AM by esterase in a viable cell emits a strong green fluorescence (excitation: 490 nm, emission: 515 nm). (dojindo.com)
Since both calcein and PI-DNA can be excited with 490 nm, simultaneous monitoring of viable and dead cells is possible with a fluorescence microscope. (dojindo.com)

In addition to a role in adaptive immunity, memory CD8+ T cells are involved in innate immunity, being able to become activated and to proliferate under cytokine stimulation (107, 108). (exposed-skin-care.net)
This includes regulating the rhythm of the body's own (innate) immune cells called macrophages. (bariatricnews.net)

However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
LipiBright™ should be reconstituted with 200 µl of anhydrous DMSO to create a 340 µM stock solution for cell imaging or with 40 µl of anhydrous DMSO to create a 1.5 mM stock suitable for tissue or small organism imaging. (cytoskeleton.com)

Cells were imaged as described in Application 1 methods. (cytoskeleton.com)

Stocks of HHV-1, pressure KOS (ATCC, VR-1493), had been produced in Vero cells. (adenosine-receptor.com)

CUT&RUN was performed with 100,000, 10,000, or 5,000 HCT 116 cells (as indicated) and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, using the CUT&RUN Assay Kit. (cellsignal.com)
CUT&RUN was performed with 100,000, 20,000, or 10,000 HCT 116 cells (as indicated) and TCF4/TCF7L2 (C48H11) Rabbit mAb #2569, using the CUT&RUN Assay Kit. (cellsignal.com)
CUT&RUN was performed with 100,000, 20,000, or 10,000 NCCIT cells (as indicated) and SUZ12 (D39F6) XP ® Rabbit mAb #3737, using the CUT&RUN Assay Kit. (cellsignal.com)

Another statement showed the preadipocyte- and endothelial cell-derived stromal-derived element-1 (CXCL12), mediated early infiltration of CD4+ T lymphocytes in obesity, which preceded the increase of macrophages in adipose cells of mice on HFD (101). (exposed-skin-care.net)
Our results add to the growing body of work showing why disruption of our body clock leads to inflammatory and infectious disease, and one of the aspects is fuel usage at the level of key immune cells such as macrophages,' said , lead author on the study, Dr George Timmons. (bariatricnews.net)
In this study, the researchers looked at these key immune cells called macrophages with and without a body clock under laboratory conditions. (bariatricnews.net)
They were interested to understand if macrophages without a body clock might use or 'metabolise' fuel differently, and if that might be the reason these cells produce more inflammatory products. (bariatricnews.net)

Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). (nature.com)

Consequently, several human diseases, including cancer and neurodegenerative disorders, are caused by defects in this highly regulated form of cell death. (nature.com)
Fig. 3: Dissection of native human hematopoiesis with multimodal cell state inference and mtDNA-based lineage tracing. (nature.com)
The five human colorectal cell lines used in this study: HT-29, DLD-1, Colo 201, LoVo, and SW620, were obtained from the Japanese Cancer Research Resources Bank (Tokyo, Japan). (biomedcentral.com)

Then stain viable cells with that Calcein-AM solution to check whether the viable cell can be stained. (dojindo.com)
The LipiBright™ probes consists of bright, fluorogenic live cell dyes that stain lipid droplets. (cytoskeleton.com)

When these cell rhythms are disrupted (due to things like erratic eating/sleeping patterns or shift work), the cells produce molecules which drive inflammation. (bariatricnews.net)

Phosphorylation or coupling control protocol (PCP, CCP) with intact cells. (bioblast.at)
The system can be used in cells, which are not permeabilized and permeabilized with digitonin as a control (Fig2). (openlabnotebooks.org)
we found that the IRS-1 overexpressing cells grow more rapidly than control cells do. (biomedcentral.com)

In wild-type cells, this is associated with caspase 12 cleavage that is abolished in bax − / − bak − / − cells. (rupress.org)

The many functions of the organism in our cells. (mitophysiology.org)

Anatomy6

Organisms1

Chemicals and Drugs12

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes4

Technology, Industry, Agriculture3

Mammalian3

Mitochondrial8

Apoptosis11

Mitochondria8

Saponin2

Permeabilization1

Assay solution1

Propidium3

Hepatocytes1

Proliferation4

Tumor3

Tissues9

Biol3

Concentration5

Cellular9

Endothelial cells2

Actin cytoskeleton1

Cholesterol1

Biology2

Lipophilic1

Apoptotic death1

Assays3

Membrane3

Protein12

Receptor5

Vitro3

Induce1

K5622

Oxidative stress3

Proteins2

Reagents3

Glucose1

Nuclei2

Cardiac2

Metabolic3

Regulation1

Cancer Cells6

CRISPR2

High doses1

Fluorescence3

Innate2

Tissue6

Methods1

ATCC1

100,0003

Macrophages4

Transposase1

Human3

Stain2

Molecules1

Control3

Caspase1

Functions1