Cells differentiate cell differentiation clone cells. Medical search. Frequent questions

In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)
First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
However, the possible differences in proliferation and differentiation capabilities among independently-derived human embryonic stem cells (hESCs) are not well known because of insufficient characterization. (biomedcentral.com)
Depletion of p27(Xic1) in X. tropicalis caused an increase in proliferation and a suppression of the neuronal differentiation marker, N-tubulin. (xenbase.org)
At the same time, we found an increase in the expression of ElrC, a marker of cells as they undergo a transition from proliferation to differentiation. (xenbase.org)
Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. (thermofisher.com)
The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. (thermofisher.com)
Cancer cells rewire the cellular metabolism, persuading it to meet new demands of growth and proliferation. (bvsalud.org)
Regardless of their polarization, microglia inhibited NSC proliferation, increased NSC migration, and accelerated NSC differentiation. (biomedcentral.com)
Tumors develop when the normal regulation of the balance between cell proliferation (mitosis) and programmed cell death (apoptosis) is lost. (doctorlib.info)
These genes are deleted or inactivated in cancer cells, allowing unregulated proliferation. (doctorlib.info)
B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
The authors then observed that only a subset of cancer stem cells in each patient was showing signs of proliferation, while all other cancer cells were not. (technologynetworks.com)
Pax6 serves as a regulator in the coordination and pattern formation required for differentiation and proliferation to successfully take place, ensuring that the processes of neurogenesis and oculogenesis are carried out successfully. (biolegend.com)
STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
Id2a-dependent defects in retinoblast proliferation lead to microphthalmia and to an absence of nearly all differentiated inner and outer nuclear layer cell types. (biologists.com)
BTK is necessary for the proliferation and the differentiation of B lymphocytes. (medscape.com)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

eCSCs are clonogenic, self-renewing and multipotent in vitro and in vivo, capable of generating the 3 major cell types of the myocardium: myocytes, smooth muscle and endothelial vascular cells. (wikipedia.org)
For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)
furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)
These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
Expression of EBF2 in the PDX1 and E-cadherin-expressing and organoid forming pancreatic epithelial CSCs indeed induced ductal and acinar-like structural differentiation in vivo in tumors resulting in tumor suppression. (amegroups.org)
This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)
To investigate whether p27(Xic1) is necessary for cell cycle exit and/or neuronal differentiation, we used antisense morpholino oligos (MO) to knockdown the protein levels in vivo. (xenbase.org)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Polarized microglia had differential effects on the differentiation potential of NSCs in vitro and in vivo, with M1 microglia promoting astrocytogenesis, while M2 microglia supported neurogenesis. (biomedcentral.com)
Furthermore, immunofluorescence staining demonstrated that the transplanted USCs differentiated into UCs and SMCs in vivo. (biomedcentral.com)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)
Modulation of LDL receptor endocytosis by Dab2 has also been studied in cultured cells [ 36 ], though a role in vivo has not yet been established. (biomedcentral.com)

Afterwards, different membrane markers (Sca-1, Abcg-2, Flk-1) and transcription factors (Isl-1, Nkx2.5, GATA4) have been employed to identify and characterize these cells in the embryonic and adult life. (wikipedia.org)
Embryonic stem cells are useful for experiments because they are self-renewing and able to develop into almost any cell type in the body. (asu.edu)
Yamanaka induced somatic cells to act like human embryonic stem cells (hESCs), allowing researchers to experiment with non-embryonic stem cells with a similar capacity as hESCs. (asu.edu)
His focus has ranged from the development of telomerase-based therapeutics to the application of human embryonic stem cells in regenerative medicine. (asu.edu)
As of 2009, West advocated using human somatic cell nuclear transfer techniques to derive human embryonic stem cells for therapeutic practice. (asu.edu)
James Alexander Thomson, affectionately known as Jamie Thomson, is an American developmental biologist whose pioneering work in isolating and culturing non-human primate and human embryonic stem cells has made him one of the most prominent scientists in stem cell research. (asu.edu)
According to the The Herald, he was the first, alongside his colleague Martin Evans, to isolate and culture embryonic stem cells. (asu.edu)
Father Tad Pacholczyk is convinced that embryonic stem cells will someday cure diseases. (archstl.org)
That's why Father Pacholczyk, director of education at the National Catholic Bioethics Center in Philadelphia, said that the efforts to help people understand the immorality of embryo reserch, including human cloning, must focus on humanizing the issue and appreciating our own embryonic origins, not just on the desired results of embryonic or other types of stem-cell research. (archstl.org)
A decade later, cloning came to the forefront in Missouri with the narrow passage of Amendment 2, a ballot initiative in 2006 that constitutionally protects embryonic stem-cell research and human cloning. (archstl.org)
We differentiated several human embryonic stem cell lines into RPE," said Irina Klimanskaya, Senior Scientist at ACT, and first author of the study. (news-medical.net)
Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
In the ongoing debate over embryonic stem cells some have argued that the cells have not demonstrated their potential value in medicine. (news-medical.net)
Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
A unique and essential property of embryonic stem cells is the ability to self-renew and differentiate into multiple cell lineages. (biomedcentral.com)
All cell lines differentiated into three embryonic germ lineages in embryoid bodies and into neural cell lineages when cultured in neural differentiation medium. (biomedcentral.com)
Human embryonic stem cells (hESCs) possess the ability to self-renew in an undifferentiated state in culture while retaining the ability to differentiate into all of the cell types in the human body. (biomedcentral.com)
In the present study, a side-by-side comparison of the ability to maintain an undifferentiated state and to self-renew under standard conditions, the ability to spontaneously differentiate into cell types of three germ layers in embryonic bodies, and directed differentiation under neural differentiation-promoting conditions was made between three NIH registered hESC lines I3, I6 and BG01V. (biomedcentral.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
Conditional deletion indicates that Dab2 is dispensable for organ development, when the vast majority of the embryonic cells are dab2 null. (biomedcentral.com)
skin cells of the mouse into cells strikingly similar to embryonic stem cells, capable of development into a mouse. (brandeis.edu)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

However, they have not changed the well-established fact that the working myocardium is mainly constituted of terminally differentiated contractile myocytes. (wikipedia.org)
Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
The frequency and activity of terminally differentiated NKG2a/c low CD16 + natural killer cells correlates with a reduction of replication-competent SIV in lymph node during antiretroviral therapy and time to viral rebound following analytical treatment interruption. (nature.com)
define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. (thermofisher.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)
To validate this putative candidate gene and to explore its function, we used CRISPR/Cas9 genome editing to discover that cultured TPM1 -deficient induced pluripotent stem cells enhanced hematopoietic progenitor cell formation. (biomedcentral.com)
We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)
In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)

Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
We identified 24 common genes that were significantly altered in both PMNs and PBMCs, including genes involved in IFN signaling that were downregulated after treatment in both cell populations, and IL1R1 that was upregulated. (frontiersin.org)
Identifying causal variants and genes from human genetic studies of hematopoietic traits is important to enumerate basic regulatory mechanisms underlying these traits, and could ultimately augment translational efforts to generate platelets and/or red blood cells in vitro. (biomedcentral.com)
To identify putative causal genes from these data, we performed computational modeling using available genome-wide association datasets for platelet and red blood cell traits. (biomedcentral.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The protein products of tumor suppressor genes are normal genes that slow down cell division, repair DNA mistakes, and tells when to undergo apoptosis (programmed cell death). (doctorlib.info)
P53 is a tumor suppressor gene that codes for a transcription factor that regulates the expression of other genes and arrests the cell cycle. (doctorlib.info)
Furthermore, specific genes have been found that are necessary for normal patterns and numbers of cell divisions. (caltech.edu)
Molecular cloning of these genes is revealing the molecular basis of plant cell division control. (caltech.edu)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
Their analysis of more than 4,000 individual tumor cells, the largest effort to date in brain tumors, finds three developmental categories of cancer cells - one resembling neural stem cells and two characterized by sets of genes indicting paths towards differentiation. (technologynetworks.com)
The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
His demonstration that the expression of four master regulatory genes was sufficient to cause the reprogramming of adult cells has opened up many possibilities for human stem cell therapies. (brandeis.edu)
Early stages of B-cell differentiation can be identified by the status of the immunoglobulin genes and by the cell surface markers CD34, CD19, and surface immunoglobulin (sIg). (medscape.com)
Genes required for B cell development. (medscape.com)
Gene Therapy Although gene therapy is defined as any treatment that changes gene function, it is often thought of as the insertion of normal genes into the cells of a person who lacks such normal genes because. (msdmanuals.com)
Genes and Chromosomes Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or the code for functional ribonucleic. (msdmanuals.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

In pluripotent and differentiated cells HP1β is differentially localized and differentially associated with chromatin. (biomedcentral.com)
CEPT improved routine cell passaging, cryopreservation/thawing of pluripotent and differentiated cells, embryoid body and organoid formation, single-cell cloning, genome editing, and new iPSC line generation. (labroots.com)

These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)

Transfer of a single nucleus at a specific stage of development, to an enucleated unfertilized egg, provided an opportunity to investigate whether cellular differentiation to that stage involved irreversible genetic modification. (todayinsci.com)
These data demonstrate that Akt2 expression is activated during cellular differentiation and suggest that it functions in the signaling pathways of some adult tissues. (nih.gov)

Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
Previous studies have found that DRDs can regulate differentiation of immune cells. (bmj.com)
Finally, in vitro experiments and RNA sequencing (RNA-seq) were used to explore the possible pathway by which DRDs regulate Tfh cell differentiation. (bmj.com)
Recently, several chromatin-related proteins have been shown to regulate ESC pluripotency and/or differentiation, yet the role of the major heterochromatin proteins in pluripotency is unknown. (biomedcentral.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
Within the central nervous system (CNS), microglia are the resident immune cells that mediate and regulate inflammatory processes. (biomedcentral.com)
Mosaic analyses reveal that Id2a function in facilitating both cell cycle progression and neuronal differentiation in the retina is non-cell-autonomous, suggesting that Id2a functions upstream of the extrinsic pathways that regulate retinogenesis. (biologists.com)

CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. (thermofisher.com)
T-cell lymphoma is a hematologic neoplasm derived from the lymphoid lineage. (bvsalud.org)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
because the embryo develops, some cells differentiate, whereas others, referred to as stem cells stay pluripotent, which suggests that they will bring about to a particular set of cell sorts referred to as a lineage. (alliedacademies.org)
Before the actual treatment, the cells are then grown to about 100 million mesenchymal stem cells which we then convert into neural progenitor cells, cells which are committed to a neural lineage (WK note: neural progenitor cells are stem cells specific to the central nervous system). (wheelchairkamikaze.com)
In particular, it remains open whether de-differentiation is prerequisite to lineage changes. (biomedcentral.com)
Recently, we have introduced a multi-scale computer model of MSC expansion, lineage commitment and differentiation which consistently explains a panel of experimental results regarding the oxygen dependence of these processes and predicts optimal culture conditions [ 14 ]. (biomedcentral.com)
According to this concept, MSC plasticity bases on permanent fluctuations of the differentiation state of each individual cell, which enables more differentiated cells to re-gain stem cell properties and subsequently to switch lineage (details see below). (biomedcentral.com)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

Drs. John B. Gurdon, Irving L. Weissman, and Shinya Yamanaka have been pioneers in studying stem cells and the reprogramming of highly differentiated adult cells into pluripotent cells capable of directing differentiation from a single cell to an adult animal. (brandeis.edu)

Bone marrow transplants are considered to be the first successful example of tissue engineering, a field within regenerative medicine that uses hematopoietic stem cells (HSCs) as a vehicle for treatment. (asu.edu)
In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)
Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Conferring cell fitness by multi-target drug combinations may become a common approach in cryobiology, drug development, and regenerative medicine. (labroots.com)
Dr. Irving L. Weissman is professor of pathology and developmental biology at the Stanford University School of Medicine , where he is director of the Institute of Stem Cell Biology and Regenerative Medicine. (brandeis.edu)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)
The mutant proteins coded by oncogenes (oncoproteins) are overactive and allow cells to proliferate when they should not. (doctorlib.info)
Every variety of cells in an exceedingly mature organism includes a distinctive assortment of proteins. (alliedacademies.org)
The blueprints for creating these proteins area unit found within the nucleus of every cell within the variety of DNA (DNA). (alliedacademies.org)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)

When cloned in suspension they form cardiospheres, which when cultured in a myogenic differentiation medium, attach and differentiate into beating cardiomyocytes. (wikipedia.org)
The aim of this study was three-fold, firstly to identify new regulators of tendon development and to analyze their expression in Meox2 mutants, secondly, to analyze the intrinsic regulation of myogenic differentiation by Meox2 transcription factor, and thirdly to establish an in vitro assay to analyze tendon fibroblast-myoblast interactions. (confex.com)
These experiments demonstrated that expression of any of the Meox variants strongly inhibited myogenic differentiation, as measured by the fusion index. (confex.com)

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. (thermofisher.com)
Mesenchymal stem cells (MSCs) are multi-potent cells that persist in adult life in some tissue types, such as bone-marrow stroma, fat, skeletal muscle, and synovium without loosing their capacity to proliferate and differentiate [ 9 , 10 ]. (biomedcentral.com)

These were not raw mesenchymal stem cells, which are the most common type of cells used in other stem cell trials and in for-profit clinics, correct? (wheelchairkamikaze.com)
Basically, in our laboratory, we take a patient's bone marrow and separate out the mesenchymal stem cells. (wheelchairkamikaze.com)
This kind of plasticity has been suggested to be a fundamental feature of mesenchymal stem cells (MSCs) as well. (biomedcentral.com)
The human bone morphogenet ic protein (rhBMP) developed by genetic engineering, was isolated by Urist, in 1965, and it is considered a substance capable of inducing differentiation of mesenchymal stem cells into osteoblasts, the cells that are responsible for the synthesis of bone matrix. (bvsalud.org)

With the myocardium now recognized as a tissue with limited regenerating potential, harbouring eCSCs that can be isolated and amplified in vitro for regenerative protocols of cell transplantation or stimulated to replicate and differentiate in situ in response to growth factors, it has become reasonable to exploit this endogenous regenerative potential to replace lost/damaged cardiac muscle with autologous functional myocardium. (wikipedia.org)
Edward Donnall Thomas, an American physician and scientist, gained recognition in the scientific community for conducting the first bone marrow transplant, a pioneering form of hematopoietic stem cell transplantation (HSCT). (asu.edu)
Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Upon transplantation into immune-deficient mice, SF3B1 mutated MDS-RS HSCs differentiated into characteristic ring sideroblasts, the hallmark of MDS-RS. (nih.gov)
Myelodysplasia syndrome is an incurable disease without hematopoietic stem cell transplantation (HSCT). (medscape.com)
At least two lumens are necessary because most children proceed to stem cell transplantation, in which intensity of treatment and blood product support necessitate multilumen vascular access. (medscape.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
BTK is critical to the maturation of pre-B cells to differentiating mature B cells. (medscape.com)

Correct development of the musculoskeletal system relies on intricate signaling pathways and gene regulation that facilitate cell differentiation. (confex.com)
Further transcriptomic analysis of isolated peripheral blood mononuclear cells (PBMCs) and PMNs from patients with acute brucellosis before treatment initiation and after successful treatment revealed a positive correlation of the molecular signature of active disease with pathways associated with response to interferons (IFN). (frontiersin.org)

Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line. (biomedcentral.com)
The immortalized hSABCi-NS1.1 cell line has diverse differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and testing new pharmacologic agents. (biomedcentral.com)
1 Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722, USA. (biologists.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)

The birth of lambs from differentiated fetal and adult cells also reinforces previous speculation that by inducing donor cells to became quiescent it will be possible to obtain normal development from a wide variety of differentiated cells. (todayinsci.com)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)

This was likely due to the fact that we used transiently transfected cells, and so we started selection of stably transfected cells to isolate clones, which show permanent, and strong gene expression to carry out future experiments. (confex.com)
However, a profound variation in colony morphology, growth rate, BrdU incorporation, and relative abundance of gene expression in undifferentiated and differentiated states of the cell lines was observed. (biomedcentral.com)
We report the isolation of a p27(Xic1) genomic clone and we identify a 5' region capable of driving reporter gene expression specifically in the neural tube and the eye. (xenbase.org)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
To more accurately reflect the mechanisms driving oligodendrogliomas, the researchers used RNA sequencing to study directly, on a single-cell level, gene expression in samples from six early-stage human tumors. (technologynetworks.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

This work not only presents a novel model to study the mechanisms of human astrocytes in vitro , but also provides an ideal platform for further interrogation of early astroglial cell autonomous events in AD and the possibility of identification of novel therapeutic targets for the treatment of AD. (nature.com)
With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
The comparison of the unique properties and behavior of each individually derived cell line is critical in identifying the safe and efficacious lines for research and therapeutic use [ 3 , 13 ]. (biomedcentral.com)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Under such circumstances, the idea of "therapeutic cloning" was proposed, indicating the generation of ESCs from SCNT embryos for therapeutic purpose. (benthamscience.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
A collaborative research effort led by scientists and engineers at the University of Pennsylvania and Northwestern University led to the development of FateMap, a framework that combines DNA barcoding, single-cell RNA sequencing (scRNA-seq), and computational analysis to study the 'fate' or outcomes after exposing cancer cells to therapeutic drugs. (cbirt.net)
Upon exposure to therapeutic drugs, resistant clones form resistant colonies and proliferate. (cbirt.net)
Through a strategic integration process, these barcoded cells are subjected to therapeutic drug treatments, with a specific focus on this study's use of vemurafenib for targeted therapy. (cbirt.net)
He was the first to appreciate the therapeutic potential of stem cell therapy and has pioneered its development. (brandeis.edu)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)
Therapeutic cloning possesses enormous potential for revolutionizing medical and thera- peutic techniques. (who.int)
This is therapeutic cloning. (who.int)
This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)

Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. (nkmaxbio.com)

The pathological potential of human astroglia in Alzheimer's disease (AD) was analysed in vitro using induced pluripotent stem cell (iPSC) technology. (nature.com)
16 Although induced pluripotent stem cell (iPSC) technology can be used to investigate human astrocyte development and function, only a limited number of studies have described homogeneous generation of healthy astrocytes. (nature.com)
Since human induced pluripotent stem cell (iPSC)-platelet products can be supplied independently from the donor, it is expected to complement current platelet products. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
Using the lentiviral transfer of OSKM, we were able to generate different iPSC clones from 3 GS-2 patients. (biomedcentral.com)
Especially in cases where sufficient cells cannot be obtained due to the rarity of the disorder, iPSC models have shown great potential in providing research material to develop new treatments or diagnostics, but also to study the pathophysiology of the diseases [ 10 , 12 ]. (biomedcentral.com)
A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)

Plasmids encoding these reporters were transfected into C2C12 myoblasts and fluorescence was analyzed as these cells underwent in vitro differentiation. (confex.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)

Our comparative analysis provides further evidence for similarities and differences between three hESC lines in self-renewal, and spontaneous and directed differentiation. (biomedcentral.com)
The generation and maintenance of replenishing tissues relies on an appropriately regulated balance between self-renewal and differentiation within a relatively small population of adult stem cells. (biomedcentral.com)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

95% homogeneous populations of human astrocytes within 30 days of differentiation from cortical neural progenitor cells (NPCs). (nature.com)

Using the same procedure, we now report the birth of live lambs from three new cell populations established from adult mammary gland, fetus and embryo. (todayinsci.com)
Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
FateMap helps identify resistant cells from subpopulations derived from single-cell (clonal) cancer populations and analyze their unique characteristics. (cbirt.net)
In vitro cultivated stem cell populations are in general heterogeneous with respect to their expression of differentiation markers. (biomedcentral.com)
Simulation results on the composition of the functional stem cell pool of MSC populations suggest that most of the cells that constitute this pool originate from more differentiated cells. (biomedcentral.com)
However, the organisation of stem cell populations is strongly influenced by environmental factors such as specific cell-cell interactions, growth factor and oxygen supply, as well as the geometry and mechanical properties of the local environment [ 3 , 4 ]. (biomedcentral.com)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)

Natural killer (NK) cells are "licensed" with functional competence following education with self-major histocompatibility complex (MHC) class I molecules 1 . (nature.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term growth and functional differentiation. (labroots.com)
Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)

Astroglia are highly heterogeneous neural cells primarily responsible for homeostasis and neuroprotection in the CNS. (nature.com)
ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)
The homogenous and heterogeneous grafts lack of living cells, since pass through a process of purification, but may show osteoconductive or osteoinductive features. (bvsalud.org)

Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

Fertilization of mammalian eggs is followed by successive cell divisions and progressive differentiation, first into the early embryo and subsequently into all of the cell types that make up the adult animal. (todayinsci.com)
The first offspring to develop from a differentiated cell were born after nuclear transfer from an embryo-derived cell line that had been induced to became quiescent. (todayinsci.com)

Depletion of the cell-cycle inhibitor p27 ( Xic1 ) impairs neuronal differentiation and increases the number of ElrC (+) progenitor cells in Xenopus tropicalis. (xenbase.org)
Effect of p27Xic1 MO on neuronal differentiation. (xenbase.org)
In a resting state, microglia are involved in the maintenance of cell homeostasis surveying their microenvironment and communicating with the neuronal tissue via extremely motile processes and protrusions [ 8 ]. (biomedcentral.com)

Unlike HIV infection, which progresses to AIDS absent suppressive anti-retroviral therapy, nonpathogenic infections in natural hosts, such African green monkeys, are characterized by a lack of gut microbial translocation and robust secondary lymphoid natural killer cell responses resulting in an absence of chronic inflammation and limited SIV dissemination in lymph node B-cell follicles. (nature.com)
While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
As a consequence, the reticuloendothelial and lymphoid organs in which these cells proliferate, differentiate, and are stored are poorly developed. (medscape.com)

These cells expressed RPE-specific molecular markers and were capable of phagocytosis, an important RPE function. (news-medical.net)
Differentiomics could also be a valuable predictive tool for quality assessment of other ES cell derivatives based on their molecular signature. (news-medical.net)
Its Molecular Mechanisms the process of cell specialization throughout development is termed differentiation. (alliedacademies.org)
While studies have been performed on tracking molecular developments that take place within cells during drug treatment, the outcomes following treatment have yet to be extensively studied. (cbirt.net)

Endogenous cardiac stem cells (eCSCs) are tissue-specific stem progenitor cells harboured within the adult mammalian heart. (wikipedia.org)
The basal cells (BC) are the stem/progenitor cells of the SAE, responsible for the differentiation into intermediate cells and ciliated, club and mucous cells. (biomedcentral.com)

John D. Gearhart is a renowned American developmental geneticist best known for leading the Johns Hopkins University research team that first identified and isolated human pluripotent stem cells from human primordial germ cells, the precursors of fully differentiated germ cells. (asu.edu)
These examples indicate that both the fate and shape of differentiated plant cells can be determined by the size of their undifferentiated precursors, and that the primary effect of mutations that alter cell fate and cellular shape can be on cell size. (caltech.edu)

The Catholic Church has always held that stem-cell research and therapies are morally acceptable, as long as they don't involve the creation and destruction of human embryos. (archstl.org)
The Church also supports research and therapies using adult stem cells, which are cells that come from any person who has been born - including umbilical cord blood, bone marrow, skin and other organs. (archstl.org)
These unique capabilities make hESCs a renewable source of a wide range of cell types for potential use in research and cell-based drug screening and therapies for many diseases. (biomedcentral.com)
Are Stem Cell-Based Therapies for Parkinson's Disease Ready for the Clinic in 2016? (lu.se)
Stem cell-based therapies for Parkinson's dis- ogy company International Stem Cell Corporation ease (PD) are rapidly moving towards clinical trials. (lu.se)

Endogenous cardiac stem cells were first discovered in 2003 by Bernardo Nadal-Ginard, Piero Anversa and colleagues in the adult rat heart and since then have been identified and isolated from mouse, dog, porcine and human hearts. (wikipedia.org)
The HeLa cell line was the first immortal human cell line that George Otto Gey, Margaret Gey, and Mary Kucibek first isolated from Henrietta Lacks and developed at The Johns Hopkins Hospital in Baltimore, Maryland, in 1951. (asu.edu)
An immortal human cell line is a cluster of cells that continuously multiply on their own outside of the human from which they originated. (asu.edu)
Scientists use immortal human cell lines in their research to investigate how cells function in humans. (asu.edu)
Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)
However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
The subject of human cloning has been around for much of the 20th century and beyond. (archstl.org)
We isolated CSCs from low passage PDAC cell lines derived from human tumors and commercially available cell lines. (amegroups.org)
CRISPR/Cas9-mediated TPM1 knockout in human induced pluripotent stem cells (iPSCs) enhanced hematopoietic progenitor development, increasing total megakaryocyte and erythroid cell yields. (biomedcentral.com)
The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
However, the derivation of human NT-ESCs goes with the destruction of clone embryos, leading to fierce ethical disputes. (benthamscience.com)
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
We recently reported the development of a new small molecule cocktail that can be used as a universal end-to-end solution for stress-free culture of human cells. (labroots.com)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
and human chorionic gonadotropin (hCG), seen in choriocarcinoma testicular germ cell neoplasms. (doctorlib.info)
In 2014, an innovative study by Leslie et al [7] used human intestinal organoids (HOIs) derived from stem cells to model the disruption of barrier functions in the human intestine by CD. (listlabs.com)
When murine erythroleukemia (MEL) cells, containing the transferred rat c‐myc gene under the control of human metallothionein II gene promoter, are induced to differentiate with dimethyl sulfoxide, the level of differentiation is dependent on the c‐Myc level, which is modulated by the addition of Zn ions. (elsevierpure.com)
The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
In their paper receiving advance online publication in Nature, a team led by investigators at Massachusetts General Hospital (MGH) and the Broad Institute of MIT and Harvard identifies for the first time cancer stem cells and their differentiated progeny in human brain tumor samples. (technologynetworks.com)
Several studies have supported a role for cancer stem cells in the aggressive brain tumors called glioblastoma, but those studies involved inducing human tumors to grow in mice, and as such their relevance to cancer in humans has been questioned. (technologynetworks.com)
Single-cell RNA-seq supports a developmental hierarchy in human oligodendroglioma. (technologynetworks.com)
His laboratory has also identified human stem cells including human leukemia stem cells that have helped to form the concept of cancer stem cells. (brandeis.edu)
Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. (thermofisher.com)
Applications Tested: The WM-59 (WM59) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Creating a human by cloning is widely seen as unethical, is illegal in many countries, and is technically difficult. (msdmanuals.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)
The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)
So long as this form of cloning (non-human) in different culture media. (who.int)
Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)

Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)

All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
Importantly, these three developmental signatures were found in distinct genetic clones - cells with identical patterns of genetic mutations - of tumor cells. (technologynetworks.com)
Activating mutations upstream may also underlie some epigenetic or within the ERK1/2 cascade are events that change cell signalling. (who.int)

Briefly, eCSCs have been first identified through the expression of c-kit, the receptor of the stem cell factor and the absence of common hematopoietic markers, like CD45. (wikipedia.org)
For instance, it was demonstrated that the combination of Flt1+/Flt4+ membrane markers identifies an Isl1+/Nkx2.5+ cell population in the developing heart. (wikipedia.org)
Using immunocytochemistry, flow cytometry, qRT-PCR and MPSS, we found that all three cell lines actively proliferated and expressed similar "stemness" markers including transcription factors POU5F1/Oct3/4 and NANOG, glycolipids SSEA4 and TRA-1-81, and alkaline phosphatase activity. (biomedcentral.com)
Anti-CD138 reacts positively with HHV8-associated primary effusion lymphoma that lack B-cell markers. (abeomics.com)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. (thermofisher.com)
The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)

Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)

The tendon fibroblasts we attempted to isolate are from Scleraxis-GFP transgenic mice, so all tendon cells are GFP positive. (confex.com)
To test this hypothesis, we used HCT116 cells, a mismatch repair-deficient cancer cell line, to isolate clones that were resistant or sensitive to bortezomib, a well-characterized proteasome inhibitor and anticancer drug to which many cancer cells possess intrinsic resistance. (bvsalud.org)

In the absence of BTK, B lymphocytes do not differentiate or mature. (medscape.com)
Without mature B lymphocytes, antibody-producing plasma cells are also absent. (medscape.com)

however, relatively little is known about these NP cell subpopulations. (biomedcentral.com)

RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)

The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)

Anatomy65

Organisms9

Diseases7

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes64

Disciplines and Occupations1

Information Science4

Proliferation21

Vivo15

Embryonic34

Terminally6

Progenitor cell6

Capacity to differentiate3

Genes23

Pluripotent and differentiated cells2

Terminal differentiation1

Cellular differentiation2

Regulate7

Lineage12

Capable of directing diffe1

Regenerative medicine10

Proteins5

Myogenic differentiation3

Proliferate and differentiate2

Mesenchymal stem4

Transplantation11

Maturation4

Pathways2

Biology7

Fetal4

Gene expression7

Therapeutic15

Spontaneously differentiate1

IPSC7

Vitro differentiation2

Self-renewal3

Neural progenitor cells1

Populations9

Functional4

Heterogeneous3

Lineages2

Embryo2

Neuronal3

Lymphoid3

Molecular4

Stem progenitor cells2

Precursors2

Therapies5

Human37

Germ layers1

Mutations3

Markers5

Endothelial Cells3

Epithelial cells2

Isolate2

Lymphocytes2

Subpopulations1

Generate1

Aberrant1