Endothelial cellsStem CellsVialSystemFibroblastsTest includes cryopreservation of culturedAssaysMethodsEmbryonicBiologyVivoGrownAbstractEndothelial2019ProliferateRegenerative medicineMorphologySecretionTumorFeeder2018IsolationLaboratoryMarket was valued at USDRenalMesenchymalStem cell resApplicationsUseful hints for culturing anScaffoldsSkeletal muscleBiochemicalResearchersProteinsCollagenInducesAssayNovel 3D cell cultureDifferentiateViableTransplantationSubstratesGrowthProtocolsFetalBehaviorTissue and organ culture2020Vitro cellWebinarFinitePluripotent cell linesSuitableReagentsMolecularAirway epithelial cells
Endothelial cells4
- We offer 4 types of normal human primary cells in the adipose cell system including adipose microvascular endothelial cells, preadipocytes-visceral, preadipocytes-subcutaneous, and also adipose-derived mesenchymal stem cells. (innoprot.com)
- Human Adipose Microvascular Endothelial Cells (HAdMEC) provided by Innoprot are isolated from human healthy adipose tissue. (innoprot.com)
- Each vial of Human Adipose Microvascular Endothelial Cells contains more than 500.000 viable cells. (innoprot.com)
- Innoprot also offers optimized medium and reagents for the growth of Human Adipose Microvascular Endothelial Cells which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro HAdMEC culture. (innoprot.com)
Stem Cells2
- Human Adipose-derived Mesenchymal Stem Cells (HAdMSC) provided by Innoprot are isolated from healthy human adipose tissue. (innoprot.com)
- Each vial of Human Adipose-derived Mesenchymal Stem Cells contains more than 500.000 viable cells. (innoprot.com)
Vial1
- Each vial of Human Preadipocytes contains more than 500.000 viable cells. (innoprot.com)
System1
- The adipose cell system stores energy, acts as an endocrine organ, insulates and protects the body. (innoprot.com)
Fibroblasts5
- New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. (springer.com)
- If additional in-house or send-out testing on cultured cells (fibroblasts) is desired, please clearly indicate the desired testing on the requisition form and call Cytogenetics lab at (614) 722-5321 and speak to a laboratory genetic counselor to coordinate testing. (nationwidechildrens.org)
- Here, we review techniques and principles involved in preparation and culturing of Big Blue ® mouse embryonic fibroblasts, treatment in vitro with chemical/physical agent(s) of interest, determination of the cII mutant frequency by the λ Select cII assay and establishment of the mutation spectrum by DNA sequencing. (mdpi.com)
- In the 1960s, biologists found that normal human fibroblasts, cells derived from connective tissue , had a predictable limit in their ability to proliferate in culture. (encyclopedia.com)
- We conclude that lyso-PC can induce growth factor gene expression in cultured endothelial cells and thus may contribute to the migration and proliferation of smooth muscle cells and fibroblasts in various response-to-injury settings in vivo. (jci.org)
Test includes cryopreservation of cultured2
Assays8
- She has worked with xenograft models and many cancer cell lines, and she has expertise in in vitro cell based functional assays and in vivo experiments. (brighttalk.com)
- In addition to spheroids, collagen-based scaffolds that encourage cell aggregation into tumoroids have been used for immunotherapy applications such as natural killer cell cytotoxicity assays. (genengnews.com)
- Physiological relevance is a key parameter to improve predictivity of cell-based assays in drug discovery and toxicity testing for compound. (dechema.de)
- In culture assays it was possible to test human and sheep agent strain combinations that could not be discriminated in animals. (medicalnewstoday.com)
- The µ-Dish 35-mm Quad from Ibidi GmbH is a four-compartment cell culture dish that can be used for simultaneous assays, guaranteeing high optical quality. (photonics.com)
- Mutation assays in cultured cells of transgenic rodents constitute, in many ways, viable alternatives to in vivo mutagenicity experiments in the corresponding animals. (mdpi.com)
- Nuclear runoff assays documented an increased transcriptional rate for the HB-EGF gene in lyso-PC-treated cells. (jci.org)
- This week (and a half) in Portland Community College's Bioscience Technology Program we continued work with assays, and had guest speakers in Current Topics and Cell Culture. (bio-link.org)
Methods22
- Over the past few years, 3D Cell Culture has gained momentum within the pharmaceutical industry due to the benefits that this model offers over conventional cell culture methods. (smi-online.co.uk)
- In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. (springer.com)
- In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. (springer.com)
- The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
- The establishment of future retinal pigment epithelium (RPE) replacement therapy is partly dependent on the availability of tissue-engineered RPE cells, which may be enhanced by the development of suitable storage methods for RPE. (hindawi.com)
- With the advancement of RPE cell replacement therapy, and with 20-25 million known sufferers from AMD worldwide [ 17 ], a great need for improved storage methods for cultured RPE is likely to emerge. (hindawi.com)
- b ) Culturing cells on 2D hydrogel films has some of the same disadvantages as conventional methods but permits user-defined control of the substrate stiffness and adhesive ligand presentation. (nature.com)
- While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. (springer.com)
- Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. (springer.com)
- Provided are systems and methods for providing human cell cultures. (google.es)
- Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development. (google.es)
- The invention relates to stem cells (SC) in particularly to methods and systems for handling human embryonic stem cells (hESC). (google.es)
- If you are adopting the next-generation technologies to improve your daily lives such as smart phones, then why stick to the old cell culture methods and compromise results in the lab? (lonza.com)
- Primary cell cultures come from the outgrowth of migrating cells from a piece of tissue or from tissue that is disaggregated by enzymatic, chemical, or mechanical methods. (qiagen.com)
- After the Second World War, methods of culturing tissue were further improved and provided the basis for a genuine mass (re)production of cells. (jhu.edu)
- Accordingly, most culture methods require sterile conditions. (encyclopedia.com)
- Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. (elsevier.com)
- Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. (elsevier.com)
- GBI Research, the leading business intelligence provider, has released its latest research report, Manufacturing of Vaccines - Cell Culture Technology Gradually Replacing Egg-Based Manufacturing, which provides insights into the production methods of vaccines and into vaccine production capacity. (mynewsdesk.com)
- Previous experience in either microengineering systems or cell culture methods would be advantageous. (strath.ac.uk)
- Protocols in this series are examples of methods for general cell culture and have not been rigorously validated and optimized by QIAGEN. (bio-medicine.org)
- Other topic areas which will be featured at Cell Culture 2012 are the challenges involved with preventing cell line cross contamination and the methods used to mitigate these risks, as well as novel methods in the use of 3D cell cultures. (selectbiosciences.com)
Embryonic22
- In 1885, Wilhelm Roux removed a portion of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the principle of tissue culture. (wikipedia.org)
- This is a hands-on, basic cell culture training course focusing on induced pluripotent stem cells (iPSC) and human embryonic stem cells (hESC). (ucl.ac.uk)
- Gibco™ Neurobasal™ Medium is a basal medium that meets the special cell culture requirements of pre-natal and embryonic neuronal cells when used with Gibco™ B-27™ Supplement. (fishersci.com)
- To address these limitations, our guest presenter and her team have established a unique model system whereby they can retro-engineer patient-specific GBMs using patient-derived glioma stem cells (GSCs) and human embryonic stem cell (hESC)-derived cerebral organoids. (brighttalk.com)
- A pioneering new study led by Florida State University biologists could fundamentally change our understanding of how embryonic stem cells differentiate into specific cell types. (news-medical.net)
- Science Daily reports that for the first time, human embryonic stem cells have been cultured under chemically controlled conditions without the use of animal substances, which is essential for future clinical uses. (slashdot.org)
- Now, for the first time, we can produce large quantities of human embryonic stem cells in an environment that is completely chemically defined," says professor Karl Tryggvason, who led the study at Sweden's Karolinska Institutet. (slashdot.org)
- IPsC are when they take cells from your own body and make them revert back to a similar state to embryonic stem cells, to where they can then be turned into any cell type you want (the advantage there being they're your cells so you wouldn't get tissue rejection like you would with embryonic stem cells. (slashdot.org)
- Over a period of 7-21 days, PGCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells termed embryonic stem and embryonic germ (EG) cells. (pnas.org)
- Throughout the culture period most cells within the colonies continued to be alkaline phosphatase-positive and tested positive against a panel of five immunological markers (SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81) that have been used routinely to characterize embryonic stem and EG cells. (pnas.org)
- Immunohistochemical analysis of embryoid bodies collected from these cultures revealed a wide variety of differentiated cell types, including derivatives of all three embryonic germ layers. (pnas.org)
- Pluripotent stem cells have been derived from two embryonic sources. (pnas.org)
- Embryonic stem (ES) cells are derived from the inner cell mass of preimplantation embryos ( 1 , 2 ), and embryonic germ (EG) cells are derived from primordial germ cells (PGCs) ( 3 , 4 ). (pnas.org)
- Other important characteristics include growth as multicellular colonies, normal and stable karyotypes, the ability to be continuously passaged, and the capability to differentiate into cells derived from all three embryonic germ layers. (pnas.org)
- This property alone may not be a definitive test of stem cell pluripotency, as it has been demonstrated that rat and mouse visceral (yolk sac) endoderm are capable of forming highly differentiated teratomas containing cells of all three embryonic germ layers ( 29 , 30 ). (pnas.org)
- Via homologous recombination in embryonic stem cells (ES cells), it is possible to generate a specific genetic modification at a specific location in the genome. (dkfz.de)
- 2. The method of claim 1 , wherein the human pluripotent stem cells are embryonic stem cells. (google.es)
- Embryonic stem cell lines derived from human blastocysts. (google.es)
- Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. (google.es)
- Feeder-free growth of undifferentiated human embryonic stem cells. (google.es)
- Embryonic stem cells differentiate more efficiently to blood-forming stem cells when cultured in three-dimensional scaffolds compared to cells cultured in two dimensions ( 2 ). (sciencemag.org)
- At the same time, he was propagating und publicizing a new image of life that could be understood as a literal embodiment of durée in the sense of philosopher Henri Bergson: in 1912, Carrel placed tissue from an embryonic chicken heart in a specifically designed flask and maintained this living culture for over 20 years. (jhu.edu)
Biology9
- He has over 25 years experience in cell biology with specific interests in cell culture technology, neuroscience and stem cell research. (smi-online.co.uk)
- Scientific advances in cancer cell biology are changing the paradigm in oncology research. (corning.com)
- Current preclinical GBM models are limited by the lack of a "normal" human microenvironment and the inability of many tumor cell lines to accurately reproduce GBM biology. (brighttalk.com)
- This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians. (wiley-vch.de)
- The market for primary cell lines is growing as such cells are thought to be more biologically relevant than other cell lines for studying human and animal biology from basic research to drug discovery. (bccresearch.com)
- Classical cell biology teaches that exocytosis causes the membrane of exocytic vesicles to disperse into the cell surface and that a cell must later retrieve by molecular sorting whatever membrane components it wishes to keep inside. (pnas.org)
- The general process of cell culture has been used extensively since the early 1900s for research on tissue growth and development, virus biology, properties of cancer cells, studies relating to aging, genetics, and gene therapy. (encyclopedia.com)
- The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (strath.ac.uk)
- We propose to apply a systems biology approach to achieve a computational representation of tissue-level physiological responses by integrating empirical data derived from organotypic culture systems with computational models of intracellular pathways to better predict human responses. (osti.gov)
Vivo14
- 3D cell culture enables cells to grow and interact with their surroundings in all three dimensions, better mimicking the in-vivo conditions in which the cells are naturally present. (smi-online.co.uk)
- Create a more in vivo-like cell culture to advance your discoveries right from the start. (corning.com)
- As research demands have become increasingly complex, we have been there every step of the way, creating 3D cell culture solutions that allow you to produce optimal environments for growing cells that exhibit in-vivo like behaviors and functionality. (corning.com)
- Cells can be isolated from tissues for ex vivo culture in several ways. (wikipedia.org)
- Quasi Vivo Systems are a series of advanced, interconnected cell culture flow systems, specifically designed to improve cellular phenotypes and human in vivo relevance of in vitro cell culture models. (sciencemag.org)
- Sartorius Stedim Biotech, a leading international supplier for the biopharmaceutical industry today announced the launch of the BIOSTAT RM TX single-use bioreactor, a new wave mixed system developed specifically for closed, automated expansion of consistent quality cell products such as ex vivo cellular immunotherapies. (news-medical.net)
- It is becoming increasingly clear that these models mimic cells in vivo at a greater capacity than traditional cell culture. (genengnews.com)
- Until now it has not been possible to establish progressively growing cultures of these cells in vitro , and cell lines have only been obtained after teratocarcinoma formation in vivo . (nature.com)
- These cells are able to differentiate either in vitro or after innoculation into a mouse as a tumour in vivo . (nature.com)
- Culture systems that better mimic the biological milieu are needed to bridge the gap between conventional cultures and complex native in vivo environments. (nature.com)
- The pluripotency of ES and EG cells can be demonstrated in vitro and in vivo . (pnas.org)
- Perhaps the most definitive in vivo test of developmental potential would be a demonstrated contribution to all cell lineages in a chimeric animal, but this test is not practical or possible for all species and cannot be done with human cells. (pnas.org)
- It uses a novel method to create high-density collagen scaffolds with cells embedded, enabling improved cellular interactions that more closely resemble an in vivo environment. (lonza.com)
- Primary cells are considered by many researchers to be more physiologically similar to in vivo cells. (qiagen.com)
Grown14
- Cells grown in vitro from neoplastic tissue. (nih.gov)
- Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (wikipedia.org)
- Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (wikipedia.org)
- Cells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) in a cell incubator. (wikipedia.org)
- 2. A culture of cells grown by this technique or process. (thefreedictionary.com)
- Interestingly, spheroids derived from stem cells grown in 3D models show improved "stemness," that is, characteristics that may lead to increased efficacy in regenerative medicine. (genengnews.com)
- The process involves taking tissue from a live animal and extracting stem cells which are then grown into muscle fibres in a bioreactor. (fao.org)
- Marianne Ellis is a Biochemical Engineer at Bath University, UK and her team has taken cells grown and matured in a bioreactor and then combined them with other ingredients to make products such as burgers, sausages and meat balls. (fao.org)
- EBs from mouse pluripotent stem cells grown on feeder layers or in suspension may contain a variety of cell types. (pnas.org)
- Studies were performed in both rabbit and rat renal PT cells grown in primary culture, as previously described. (ahajournals.org)
- Hematopoietic cells (derived from blood, spleen, or bone marrow) as well as some transformed cell lines and cells derived from malignant tumors can be grown in suspension. (qiagen.com)
- When cells have grown (by dividing) to cover the flasks' surface, the process of enzymic dissociation can be repeated and the cells replanted to additional flasks. (encyclopedia.com)
- Skin cells have been grown in the laboratory since the mid 1970s, but until now there has not been a significant scientifically controlled study. (rxpgnews.com)
- The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
Abstract1
- Abstract Cultured glomerular mesangial cells have been shown to produce a potent vasoconstrictive peptide, endothelin-1 (ET-1). (ahajournals.org)
Endothelial8
- In addition, mouse ES cells have been used to generate in vitro cultures of neurons ( 26 ), skeletal muscle ( 27 ), and vascular endothelial cells ( 28 ). (pnas.org)
- The company's exVive3D™ human liver tissue model is constructed from three basic cellular elements: primary hepatocytes, primary endothelial cells, and primary stellate cells. (genengnews.com)
- Two key aspects of this process are initial endothelial cell (EC) dysfunction and subsequent phenotypic change and accumulation of smooth muscle cells (SMCs), with inherent disruption in local cell-cell communication. (strath.ac.uk)
- Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. (jci.org)
- In this study, we have found that lyso-PC increased steady state mRNA levels for two smooth muscle/fibroblast-directed growth factors, the A and B chains of PDGF and heparin-binding EGF-like protein (HB-EGF), in cultured human endothelial cells. (jci.org)
- Lyso-PC did not upregulate the expression of certain other inducible endothelial genes, including E-selectin, IL-8, or monocyte chemoattractant protein-1 in the same cells, in contrast to the coordinate pattern of activation typically observed with other stimuli, such as TNF alpha, bacterial endotoxin, or PMA. (jci.org)
- Endothelin-1 (ET-1) is a vasoconstrictive peptide of 21 amino acids that was first isolated from porcine vascular endothelial cells. (ahajournals.org)
- 8 11 12 In human endothelial cells, phorbol esters, activators of PKC, cause a marked and immediate stimulation of ET-1 production, accompanied by induction of preproendothelin mRNA. (ahajournals.org)
20192
- Join us in February 2019, as we look at the latest developments in the 3D Cell Culture field. (smi-online.co.uk)
- According to Verified Market Research, Global Primary Cell Culture Market was valued at USD 2.49 Billion in 2018 and is projected to reach USD 5.3 Billion by 2026, growing at a CAGR of 9.84% from 2019 to 2026. (bccresearch.com)
Proliferate7
- An established or immortalized cell line has acquired the ability to proliferate indefinitely either through random mutation or deliberate modification, such as artificial expression of the telomerase gene. (wikipedia.org)
- This group has recently shown that epithelial cells extracted from the developing teeth of 6-month-old pigs continue to proliferate when they are cultured on top of a special feeder layer of cells (the feeder-layer cells are known as the 3T3-J2 cell line). (eurekalert.org)
- For example, human breast epithelial cells proliferate abnormally (like tumor cells) when cultured as a two-dimensional monolayer, but display normal cell growth behavior and form structures typical of breast tissue when cultured in three-dimensional membranes that resemble their native environment ( 1 ). (sciencemag.org)
- Suspension cells can survive and proliferate without being attached to a substratum. (qiagen.com)
- Primary cultures are formed from cells that survive the disaggregation process, attach to the cell culture vessel (or survive in suspension), and proliferate. (qiagen.com)
- These cultures will proliferate for a limited number of cell divisions, after which they will senesce. (qiagen.com)
- The cells will proliferate for an extended time, but usually the culture will eventually cease dividing, similar to senescent primary cells. (qiagen.com)
Regenerative medicine3
- A team of scientists led by Prof. Antonella Consiglio from the IDIBELL and the University of Barcelona, and Prof. Angel Raya from the Center of Regenerative Medicine of Barcelona have discovered that defective versions of human brain cells called astrocytes are linked to the buildup of a toxic protein that is one the hallmarks of Parkinson's disease. (news-medical.net)
- The book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover and therefore delivers in a compact and clear way important theoretical as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. (powells.com)
- Naturally based" hydrogels such as Matrigel (made of native extracellular matrix proteins) and collagen (the major extracellular matrix protein) have been explored extensively for applications in three-dimensional tissue culture and regenerative medicine. (sciencemag.org)
Morphology3
- b ) Mouse MSCs cultured in 3D alginate hydrogels display rounded morphology regardless of substrate stiffness. (nature.com)
- Conventional 2D culture conditions are highly limited as native tissue morphology and cell composition is not properly reflected. (dechema.de)
- Another difficulty with some cultured cells is their tendency to change their morphology , functions, or the range of genes they express. (encyclopedia.com)
Secretion7
- In summary, these results confirm the presence of renin mRNA in cultured proximal tubular cells and suggest that a low-level, constitutive secretion of renin occurs in this system that is decreased by A23187. (ahajournals.org)
- Renin secretion from JG cells has been well described: Renin release from these cells is largely regulated by intracellular calcium and cAMP, such that a decline in intracellular calcium or an increase in cAMP stimulates renin secretion in JG cells. (ahajournals.org)
- We have investigated the hypothesis that fluid secretion by these cells is dependent on cAMP-mediated chloride secretion. (nih.gov)
- The rate of fluid secretion, cell volume and changes in intracellular Cl- were measured. (nih.gov)
- These data indicate that cultured ADPKD cells can direct fluid transport in either the absorptive or the secretory direction, and that cAMP stimulates secretion and this secretion is accompanied by a net loss of cell solute. (nih.gov)
- These data support the thesis that chloride secretion drives fluid secretion by cultured ADPKD cells. (nih.gov)
- 3 4 5 Previous studies demonstrated constitutive expression of ET-1 transcripts and peptide secretion in cultured glomerular mesangial cells. (ahajournals.org)
Tumor1
- If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (nih.gov)
Feeder10
- Delegates will leave the module with practical knowledge to propagate, maintain and characterize feeder and feeder-free stem cell lines. (ucl.ac.uk)
- Lecture 5: The effect of reduced oxygen tension on feeder and feeder free culture of human pluripotent stem cells. (ucl.ac.uk)
- Essential 8™ Flex is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) without the need for weekend feeding. (fishersci.com)
- The idea for the culturing technique originates from 1975, when Dr. J.G. Rheinwald and Dr. H. Green of Harvard Medical School reported the use of feeder layers for culturing epithelial cells from the skin (the 3T3-J2 cells used in the current study were gifted by Dr. Green). (eurekalert.org)
- culturing them required feeder cells which the article describes why that's bad, and 3. (slashdot.org)
- With the goal of establishing pluripotent stem cell lines, gonadal ridges and mesenteries containing primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor, human recombinant basic fibroblast growth factor, and forskolin. (pnas.org)
- 4. The method of claim 1 , wherein the human umbilical cord feeder cells consist of 100% human umbilical cord fibroblast feeder cells. (google.es)
- 7. The method of claim 6 , wherein the human umbilical cord feeder cells consists essentially of the human umbilical cord fibroblast feeder cells. (google.es)
- When using this complete media system, the growth of airway epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (atcc.org)
- Using Renal Epithelial Cell Basal Medium supplemented with the Renal Epithelial Cell Growth Kit, the growth of renal epithelial cells is supported without the use of feeder layers, extracellular matrix proteins or other substrates. (atcc.org)
20182
- We look forward to meeting you at 3D Cell Culture 2018 in Freiburg! (dechema.de)
- 2018. "Mutation Analysis in Cultured Cells of Transgenic Rodents. (mdpi.com)
Isolation5
- Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. (springer.com)
- She has extensive experience in a variety of cell culture techniques including isolation and culture of primary patient cells. (brighttalk.com)
- Later: Freezing and DNA isolation of part of the ES cell clones. (dkfz.de)
- This test is intended for amniotic fluid samples that does not have any accompanying cytogenetic test order (e.g. chromosome analysis) but cell culture is desired for other molecular genetic or biochemical testing, such as familial mutation analysis, DNA isolation and storage, and send-out testing for molecular genetic/biochemical testing to be done on cultured amniocytes. (nationwidechildrens.org)
- Finally, many standard techniques for analyzing proteins and protein distributions are more difficult to perform, because they require isolation of the cells from the matrix. (sciencemag.org)
Laboratory12
- The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
- It will also provide managers with insights into the intricacies of cell culture and the necessary laboratory infrastructure requirements. (ucl.ac.uk)
- The Genomics building has two fully equipped rooms dedicated to cell culture, which are part of the second and third floor laboratory areas. (concordia.ca)
- The idea of developing a laboratory instrument that handles and sorts individual, viable cells has been realized by researchers in the Laboratory for MEMS Applications in the Department of Microsystems Engineering at the University of Freiburg. (news-medical.net)
- In a search for alternative sources of meat and in response to concerns over meat consumption, animal welfare and greenhouse gas emissions, scientists have developed the ability to grow meat from animal cells in a laboratory. (fao.org)
- Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines. (routledge.com)
- Cell culture disposables for cell cultivation and cell monitoring in laboratory scale. (sartorius.com)
- With her book Culturing Life , Hannah Landecker contributes an important chapter to the recent history of these laboratory fractals. (jhu.edu)
- Cell culture describes the laboratory growth of cells derived from plants or animals. (encyclopedia.com)
- The ability to culture cells allowed the laboratory growth of polio virus to produce vaccines that nearly eliminated polio as a disease. (encyclopedia.com)
- Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap offer a convenient solution to prepare laboratory samples. (fishersci.ca)
- 1998) Cells: a Laboratory Manual. (bio-medicine.org)
Market was valued at USD1
- The global Automated Cell Cultures Market was valued at USD 19.57 billion in 2016 and is projected to reach USD 40.65 billion by 2025, growing at a CAGR of 8.46% from 2017 to 2025. (bccresearch.com)
Renal7
- For example, renal organoids derived from pluripotent stem cells have been successfully transplanted under the renal capsules of adult mice. (genengnews.com)
- Renal Epithelial Cell Basal Medium is a sterile, phenol red-free, liquid tissue culture medium intended for use as one component in a complete ATCC ® Primary Cell Solutions™ system. (atcc.org)
- This system is designed to support epithelial cells (e.g., renal proximal tubule epithelial cells) derived from normal human kidney. (atcc.org)
- Renal Epithelial Cell Basal Medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals and inorganic salts. (atcc.org)
- Primary Renal Mixed Epithelial Cells, Normal, Human, ATCC PCS-400-012), Renal Cell Basal Medium must be supplemented with the Renal Epithelial Cell Growth Kit (ATCC PCS-400-040). (atcc.org)
- Epithelial cells cultured from the renal cysts of patients with autosomal dominant polycystic kidney disease (ADPKD) secrete fluid via a process stimulated by adenosine 3',5'-cyclic monophosphate (cAMP). (nih.gov)
- 16 Therefore, if mesangial cell production of ET-1 is enhanced in spontaneously hypertensive rats (SHR) compared with Wistar-Kyoto rats (WKY), increased endogenous ET-1 in these cells may contribute to the pathophysiology of the renal involvement associated with the progression of hypertension in SHR. (ahajournals.org)
Mesenchymal4
- In the study being reported today, the researchers seeded the cultured dental epithelial cells onto collagen sponge scaffolds, along with cells from the middle of the tooth (dental mesenchymal cells). (eurekalert.org)
- Now that dental epithelial cells can be propagated in culture, the next step will be to achieve the same success with their partners in tooth formation, the dental mesenchymal cells. (eurekalert.org)
- Researchers have seen that spheroids display enhanced anti-inflammatory, tissue regenerative, and reparative responses, as well as better post-transplant survival of mesenchymal stem cells. (genengnews.com)
- Human mesenchymal stem cells (MSCs) cultured on increasingly stiff 2D substrates display increasing spread area. (nature.com)
Stem cell res3
- BD Biosciences: Diverse set of tools for stem cell research. (ucl.ac.uk)
- Furthermore, disease research areas are expanding to include neurology, stem cell research, cell therapy , and (potentially) tissue engineering. (genengnews.com)
- As Paul Rabinow does in Making PCR: A Story of Biotechnology (1996), Landecker poses the question of how today's cell manipulations-from cloning techniques to stem cell research and reproductive medicine-have altered our understanding and experience of life. (jhu.edu)
Applications26
- Researchers globally are realizing the potential of 3D cell culture for various applications, including testing and discovering new drugs to treat cancer, organ-on-chip models to study the human physiology in an organ specific context, and 3D cell printing to produce organ models. (smi-online.co.uk)
- From product selection guides to application notes to protocols, our online resource library is available anytime you need to facilitate your cell culture applications. (corning.com)
- Rely on innovative vessels from Corning for quality and consistency in your cell culture applications. (corning.com)
- One complication of these blood-derived ingredients is the potential for contamination of the culture with viruses or prions, particularly in medical biotechnology applications. (wikipedia.org)
- It is available with three different culture chambers (QV500, QV600, and QV900) to support a wide range of applications, including submerged cell culture, coculture, and modeling of air-liquid and liquid-liquid interfaces. (sciencemag.org)
- Corning™ Untreated 245mm Square Low Profile BioAssay Dishes provide a superior option for applications where cell attachment is not desired. (fishersci.com)
- Using Nunc Glass Bottom Dishes in your research applications gives you the flexibility to convert your culture device into an imaging device. (fishersci.com)
- Gibco™ DMEM, Powder, High Glucose, No Sodium Bicarbonate Buffer is used in cell culture processing applications. (fishersci.com)
- Cell culture is a fundamental technique for a variety of applications. (brighttalk.com)
- And in therapeutics, 3D cell culture promises to realize several exciting applications, from patient-specific drug-efficacy screening to the manufacturing of bioprinted organs. (genengnews.com)
- GEN: Are 3D cell culture models as strongly focused as ever on drug safety testing, or are they finding new applications? (genengnews.com)
- We would argue that 3D cell culture models have been used for many years in basic research and disease modeling, notably in cancer research-this was, after all, one of the original applications of Corning® Matrigel®, a naturally occurring extracellular matrix for us in 3D cell culture. (genengnews.com)
- Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. (wiley-vch.de)
- Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online.Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. (wiley-vch.de)
- Fortunately, bioprinters are becoming commonplace, and the applications that they enable-such as 3D cell cultures-are starting to go mainstream. (genengnews.com)
- Click here to register for free and to view the entire webinar: http://bit.ly/kosi_136 Since 2008 Kaiser Optical has worked closely with industry leading bioprocessing companies to develop a Raman solution enabled to deliver in situ, real-time measurement to a host of bioprocessing applications including but not limited to cell culture, fermentation, and purification. (environmental-expert.com)
- Since our first symposium in 2009, the 3D Cell Culture conference series has developed into a 'place to be' for experts active in this field, and will highlight successful applications and newest developments. (dechema.de)
- Success stories of 3D culture applications will be presented as well as newest technologies and innovations. (dechema.de)
- Relevant disease models, applications of 3D models in clinic and industry, predictive cell models for compound characterization and enabling technologies will be covered. (dechema.de)
- This textbook provides an overview on current cell culture techniques, conditions and applications, it specifically focuses on human cell culture. (powells.com)
- Optimization of cell culture conditions is necessary to ensure that cells are healthy and in optimal condition for downstream applications. (qiagen.com)
- Corning™ Stripette™ Individually Wrapped Plastic or Paper/Plastic Disposable Polystyrene Serological Pipets are ideal for sterile tissue culture applications, especially while wearing latex gloves, as the wrap reduces static cling. (fishersci.ca)
- These disposable, space saving containers are specifically designed for optimal plant cell culture applications. (mpbio.com)
- The 'Recombinant Cell Culture Supplements' market can be divided based on product types and It's sub-type, major applications and Third Party usage area, and important regions. (openpr.com)
- This Request for Applications (RFA), Papillomavirus In Vitro Cell Culture Systems, is related to the priority area of sexually transmitted diseases. (nih.gov)
- ELIGIBILITY REQUIREMENTS Applications may be submitted by domestic and foreign for-profit or nonprofit organizations, public and private, such as universities, medical colleges, hospitals, laboratories, units of State and local governments, and eligible agencies of the Federal government having adequate resources or access to resources to support papillomavirus in vitro cell culture research. (nih.gov)
Useful hints for culturing an2
- This section provides useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects). (qiagen.com)
- Part V: Counting cells Welcome to the fifth of a series of articles providing useful hints for culturing animal cells. (bio-medicine.org)
Scaffolds1
- The scaffolds were then transferred into the abdominal cavities of rats, where conditions were favorable for the cells in the scaffolds to interact and develop. (eurekalert.org)
Skeletal muscle1
- Pagina 621 - Jessell TM, Siegel RE, and Fischbach GD (1979) Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. (google.it)
Biochemical1
- This test is intended for tissue samples that do not have any accompanying cytogenetic test order (e.g. chromosome analysis or microarray analysis) but needs to be cultured for other molecular genetic or biochemical testing (e.g. (nationwidechildrens.org)
Researchers7
- Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today. (springer.com)
- Today, during the 85thth General Session of the International Association for Dental Research, a team of researchers from the Institute of Medical Science, the University of Tokyo (Japan), reports on a new technique for culturing cells that have the capacity to produce enamel. (eurekalert.org)
- UPM launches GrowDex-T - a new hydrogel offering researchers exceptional imaging properties for visualization of cells in 3D. (news-medical.net)
- Although most scientists concede that standard cell culture materials (tissue culture plastic and glass) do a poor job of recapitulating native cellular milieus, there is currently a knowledge barrier for many researchers in regard to the application of hydrogels for cell culture. (nature.com)
- Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study. (springer.com)
- With the increasing incidence of neurodegenerative disorders such as Alzheimer's disease, multiple sclerosis, and Parkinson's disease, researchers are testing 3D cellular models that could be superior to animal studies because the cells are derived from humans instead of rodents. (genengnews.com)
- Researchers are now bridging the gap between natural and synthetic gels by combining well-characterized synthetic materials with biomimetic cues to support physiologically relevant cell-gel interactions (see the figure). (sciencemag.org)
Proteins8
- A Spinner is a type of bioreactor which features an impeller, stirrer or similar device to agitate the contents (usually a mixture of cells, medium and products like proteins that can be harvested). (wikipedia.org)
- There have been some breakthroughs on 1, last I heard a group had shown you can just culture with modified proteins to induce pluripotency. (slashdot.org)
- Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses. (routledge.com)
- One simple method to detect maintained adherence of cells is the staining of attached cells with crystal violet dye, which binds to proteins and DNA. (chemie.de)
- Physiological processes are guided by interactions between cells and their extracellular matrix, the proteins and polysaccacharides that cells secrete into their environment to support tissue structure and survival. (sciencemag.org)
- The advantages of synthetic gels include their consistent composition and predictable manipulation of properties, but they lack functional sites to interact with soluble or cell-surface proteins. (sciencemag.org)
- The second approach explored how the kinetics of amperometric spikes changed in intact cells while stimulus patterns varied ( 8 ), or in permeabilized cells while they overexpressed proteins involved in exo- and endocytosis ( 9 ). (pnas.org)
- d) repeating steps (b) and (c) as necessary to produce extracellular matrix proteins in the three dimensional stromal culture. (google.com)
Collagen1
- Individual cultured ADPKD cells were suspended within a polymerized collagen matrix and stimulated to form cysts. (nih.gov)
Induces1
- Oxidative Stress Induces Senescence in Cultured RPE Cells, Open Neurol. (eurekalert.org)
Assay4
- The effects of these agents on renin mRNA were examined in rabbit and rat proximal tubular cells in primary culture with the use of an S 1 nuclease protection assay. (ahajournals.org)
- This study, reported in the journal Science, duplicated and extended results of the animal experiments in a neural cell culture assay. (medicalnewstoday.com)
- In the first, exocytosis of single granules was detected as step increases in membrane capacitance, an assay of cell surface area. (pnas.org)
- Furthermore, we highlight representative studies in which the Big Blue ® mouse cell culture model and the λ Select cII assay have been used for mutagenicity testing of diverse carcinogens. (mdpi.com)
Novel 3D cell culture1
- Network with industry experts, gain insight into how the pharmaceutical industry is utilizing 3D Cell Culture technologies to enhance research and development, discuss the potential for novel in-vitro cell culture models to replace animal models, and discover novel 3D cell culture systems, organ-on-chip, 3D imaging of organoids and other technologies in development. (smi-online.co.uk)
Differentiate2
- it's hard to completely differentiate a population of pluripotent cells into one cell type you're trying to get. (slashdot.org)
- And each different cell type seems to differentiate in different ways. (slashdot.org)
Viable2
- 2D cell culture has had an extraordinary place in cell culture history and will remain a viable cell culture model for many uses. (corning.com)
- Due to strict regulatory demands [ 18 , 19 ], the development of a suitable storage method will be essential to enable the transportation of viable cell constructs from centralized laboratories to operating theatres [ 18 ]. (hindawi.com)
Transplantation5
- For this reason, it is exciting to consider the prospect of artificially growing enamel, or even whole teeth, using culturing and transplantation techniques. (eurekalert.org)
- With the demonstration of long-term survival of RPE cell transplants both in various animal models and in humans [ 8 - 13 ], transplantation offers the prospect of a single intervention cure. (hindawi.com)
- The transplantation of RPE grafts enables appropriate implantation and orientation of an organized RPE cell layer in the retina [ 5 , 14 , 15 ] and circumvents several of the complications associated with the use of RPE cell suspensions [ 6 , 8 , 15 , 16 ]. (hindawi.com)
- Autologous tissue for transplantation may also come from organoids produced via 3D cell culture. (genengnews.com)
- The cover can be snapped on tight, for long-term culture, or loosely fitted during acclimation to external conditions prior to transplantation. (mpbio.com)
Substrates1
- a ) Conventional 2D culture on superphysiologically stiff plastic or glass substrates leads to cells displaying aberrant phenotypes. (nature.com)
Growth44
- Professor Przyborski is developing new and innovative ways to manage the growth and function of cultured cells. (smi-online.co.uk)
- however, only the white cells are capable of growth in culture. (wikipedia.org)
- Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
- Aside from temperature and gas mixture, the most commonly varied factor in culture systems is the cell growth medium. (wikipedia.org)
- In addition, the third floor culture room is equipped with one lighted incubation shaker and a Coy gloveless anaerobic chamber which was converted into a light chamber (owned by the Martin lab) for the growth of photosynthetic organisms. (concordia.ca)
- The New Brunswick Innova 4430 incubation shakers are used for the growth of microbial cultures in liquid media. (concordia.ca)
- Tissue-culture (TC) treated for optimum attachment and growth of anchorage-dependent cells. (fishersci.com)
- Used in the growth of neuronal cells from hippocampus, cortex and otregions of the brain. (fishersci.com)
- Cell culture is set to see huge increases in growth including the recent advances in techniques such as 3D cell culture. (smi-online.co.uk)
- The global market for 3D cell cultures is poised for explosive growth. (genengnews.com)
- How do I know the minimum of cells for a specific cell line that give a quick entry into log phase growth? (physicsforums.com)
- Plate different dilutions of your cell line and see were the cut off point is, usually theres a treshold were the cells are suddenly to sparsely plated to give rise to normal growth. (physicsforums.com)
- The Ph+ feature avoids any meniscus effect and facilitates homogenous cell growth. (photonics.com)
- 2. The cell growth apparatus according to claim 1, wherein each compartment comprises opposing gas permeable, liquid impermeable surfaces. (freepatentsonline.com)
- 3. The cell growth apparatus according to claim 1, wherein each compartment comprises a plurality of gas permeable, liquid impermeable surfaces which form the expandable walls of the compartment. (freepatentsonline.com)
- 4. The cell growth apparatus according to claim 1, wherein the gas permeable, liquid impermeable surfaces are selected from the group consisting of polystyrene, polypropylene, polyethylene, polycarbonate, silicone rubber, fluoroethylenepropylene copolymer and a combination thereof. (freepatentsonline.com)
- 6. The cell growth apparatus according to claim 1, wherein the spacer material is selected from the group consisting of a woven mesh and a planar sheet comprising protruding support features. (freepatentsonline.com)
- 7. The cell growth apparatus according to claim 1, further comprising a support structure external to the compartments. (freepatentsonline.com)
- 8. The cell growth apparatus according to claim 7, wherein the support structure comprises a compartment frame perimetrically surrounding each of the compartments for supporting the gas permeable, liquid impermeable surfaces. (freepatentsonline.com)
- 9. The cell growth apparatus according to claim 7, wherein the support structure comprises a top platen and a bottom platen confining the plurality of compartments there-between. (freepatentsonline.com)
- 10. The cell growth apparatus according to claim 7, wherein the support structure is optically transparent. (freepatentsonline.com)
- 11. The cell growth apparatus according to claim 9, wherein the support structure further comprises at least one rod inserted through a supported hole in at least one of the compartments and attached to the top platen and the bottom platen. (freepatentsonline.com)
- 12. The cell growth apparatus according to claim 10, wherein the rod is telescoping to form an expandable support structure. (freepatentsonline.com)
- 13. The cell growth apparatus according to claim 1, wherein the interconnecting passageway comprises ports in the gas permeable, liquid impermeable surface of each compartment. (freepatentsonline.com)
- This protocol describes a quick and reliable screening method that is suitable for the examination of the impact of chemotherapeutics or other compounds on cell survival and growth inhibition. (chemie.de)
- From cell preparation and growth to investigation and analysis, our comprehensive portfolio provides the quality and consistency to ensure the validity of your results. (sigmaaldrich.com)
- Primary Bronchial/Tracheal Epithelial Cells, Normal, Human, ATCC PCS-300-010), supplement Airway Epithelial Cell Basal Medium with the Bronchial Epithelial Cell Growth Kit (ATCC PCS-300-040). (atcc.org)
- Different cell types vary greatly with respect to their growth behavior and nutritional requirements. (qiagen.com)
- Growth, Nutrition, and Metabolism of Cells in Culture, Volume 1, summarizes the state of knowledge of the growth, nutrition, and metabolism of various types of cell cultures. (elsevier.com)
- Dispersed cells are then transferred to a suitable growth medium and allowed to attach to the surface of culture flasks. (encyclopedia.com)
- Cell culture requires careful attention to the growth medium to ensure cells are given all the components they require to grow. (encyclopedia.com)
- Often the culture medium requires growth factors or hormones to stimulate growth. (encyclopedia.com)
- Media used to propagate cells are rich in nutrients and, therefore, support growth of a multitude of organisms. (encyclopedia.com)
- Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. (elsevier.com)
- Corning™ Falcon™ Tissue Culture Treated Flasks features vacuum-gas plasma tissue culture treatment to ensure consistent growth surface. (fishersci.ca)
- Designed to improve usability while maintaining the same environment for cell growth as previous designs. (fishersci.ca)
- Designed to support cell culture growth and biomanufacturing processes. (fishersci.ca)
- Resuspend the cells in an appropriate volume of prewarmed growth medium. (bio-medicine.org)
- The volume and the dry weight of a Tetrahymena cell are determined by the particular medium and the growth temperature. (biomedsearch.com)
- Large cells are seen in concentrated media and at low growth temperatures resulting in cell volumes up to 17,000 microns 3, whereas starving cells decrease gradually towards 700 microns 3 or even smaller sizes. (biomedsearch.com)
- Cell growth kinetics, division asymmetry and volume control at division in the marine dinoflagellate. (biomedsearch.com)
- Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. (google.it)
- All plant cell cultures in the PCCL are categorized into three levels of growth, High , Medium and Low . (umass.edu)
- In the absence of the latter signal, the T cell makes only a partial response and, more importantly, enters an unresponsive state known as clonal anergy in which the T cell is incapable of producing its own growth hormone, interleukin-2, on restimulation. (sciencemag.org)
Protocols8
- Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. (springer.com)
- Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells. (springer.com)
- New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. (wiley-vch.de)
- The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. (springer.com)
- It covers different types of animal cell cultures, considerations for cell culture, and cell culture protocols. (qiagen.com)
- A do-it-yourself manual for culturing nerve cells, complete with recipes and protocols. (google.it)
- They provide detailed protocols and describe their cultures in practical terms, from when the cells are first plated through the various phases of their development. (google.it)
- A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. (worldcat.org)
Fetal3
- If cultured amniocytes will be used to perform fetal molecular genetic testing, submission of a maternal blood sample (4 mL in EDTA tube) is highly recommended to perform maternal cell contamination study. (nationwidechildrens.org)
- Future Meat does not use animal serum (the liquid part of blood) in its process (some start-ups growing cultured beef, for example, began by using fetal bovine serum, a byproduct of the livestock industry, although they have since claimed to have validated animal-free alternatives). (foodnavigator.com)
- 8 11 The isolated glomeruli were then cultured in RPMI 1640 medium containing 20% fetal calf serum (FCS) and antibiotics. (ahajournals.org)
Behavior3
- There is growing appreciation of the role that the extracellular environment plays in regulating cell behavior. (nature.com)
- Therefore, we performed the present studies to learn whether the behavior of the renin system in cultured proximal tubule is similar to that of the juxtaglomerular renin system. (ahajournals.org)
- This difference in cell behavior has constituted a major obstacle for tissue engineers. (sciencemag.org)
Tissue and organ culture3
- Plant cell, tissue and organ culture. (worldcat.org)
- Plant Cell, Tissue and Organ Culture - incl. (springer.com)
- Plant Cell, Tissue and Organ Culture (PCTOC: Journal of Plant Biotechnology) details high-throughput analysis of gene function and expression, gene silencing and overexpression analyses, RNAi, siRNA, and miRNA studies, and much more. (springer.com)
20201
- The cell culture market is set to be worth £4.5 billion by 2020 driven by advances in pharma and biotech. (smi-online.co.uk)
Vitro cell1
- To create tissue-specific structures and function in vitro, cell models have to be translated into the third dimension. (dechema.de)
Webinar1
- Please view our archived webinar and learn how the new RAFT™ 3D Cell Culture System can elevate your cell culture. (lonza.com)
Finite5
- Primary cells, finite cultures, and continuous cell lines differ in their proliferative potential (see below). (qiagen.com)
- Finite cell cultures are formed after the first subculturing (passaging) of a primary cell culture. (qiagen.com)
- The proliferative potential of some human finite cell cultures can be extended by introduction of viral transforming genes (e.g., the SV40 transforming-antigen genes). (qiagen.com)
- The phenotype of these cultures is intermediate between finite cultures and continuous cultures. (qiagen.com)
- Finite cell cultures will eventually either die out or acquire a stable, heritable mutation that gives rise to a continuous cell line that is capable of unlimited proliferative potential. (qiagen.com)
Pluripotent cell lines1
- We report here the establishment in tissue culture of pluripotent cell lines which have been isolated directly from in vitro cultures of mouse blastocysts. (nature.com)
Suitable2
- This will provide novel mechanistic insights into cardiovascular disease and a well-characterised in vitro model of vascular cell-cell communication suitable for upscaling into an array format for multiplexed, label-free drug screening. (strath.ac.uk)
- The plan is to release hybrid products combining plant-based and cell based meat in 2021 and a second line of 100% cultured ground meat products suitable for things like burgers and nuggets at a cost of " less than $10 per pound " by 2022 (current small-scale production costs are c. $150/lb for chicken and $200/lb for beef). (foodnavigator.com)
Reagents3
- Although the simplicity of these platforms is attractive, cells cultured in these environments tend to display aberrant behaviors: flattened shape, abnormal polarization, altered response to pharmaceutical reagents, and loss of differentiated phenotype ( Fig. 1 ). (nature.com)
- The subdivisions enable up to four parallel, individual experiments in one single dish, which saves time and decreases experimental costs by reducing cell numbers and the amount of reagents needed. (photonics.com)
- cellTRAY forms the basis for lab-on-a-chip technology as it provides the means to automatically dispense and treat cells continually with reagents and cell culture media. (nsti.org)
Molecular2
Airway epithelial cells3
- This serum-free system is designed to support airway epithelial cells derived from normal human lung. (atcc.org)
- For airway epithelial cells derived from bronchial/tracheal tissue (e.g. (atcc.org)
- For small airway epithelial cells (e.g. (atcc.org)