Cells cell cycle arrest and apoptosis cell cycle checkpoints. Medical search. Frequent questions

The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Extracts prepared from Trametes versicolor under the trade name of I'm-Yunity exhibit anti-tumorigenic activities, as supported by inhibition of the proliferation and induction of apoptosis in malignant cells. (spandidos-publications.com)
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
Methods used to study the cytotoxic effects include cytotoxicity assay, double staining cell morphological analysis, cell cycle analysis, apoptosis test and cell proliferation assay. (upm.edu.my)
Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
Immunohistochemical analysis of paired tumor biopsies revealed increased nuclear accumulation of tumor suppressor proteins (TSPs), decreased cell proliferation, and increased tumor cell apoptosis after treatment. (karyopharm.com)
Among them, compounds 20e and 25a displayed not only high biochemical activity (IC50 = 2.99 nM and 5.91 nM vs 11.36 nM), but also higher inhibitory effects against proliferation of BRCA1-deficient UWB1.289 cells than olaparib (IC50 = 0.27 µM and 0.41 µM vs 0.66 µM). (bvsalud.org)
Mechanism study revealed that compound 8h could suppress the HepG2 cell proliferation via prompting the acetylation of histone 3 (H3) and α-tubulin, and activating the p53 signal pathway as designed. (bvsalud.org)
p53 binding decreases its control with cell proliferation and increases genetic instability, leading to neoplastic transformation. (nordiqc.org)
Number 2 Subcellular distribution of the TIA1 isoforms Involvement of TIA1 in ESCC cell proliferation To gain insight into the potential function Lidocaine (Alphacaine) of TIA1 whose overexpression could be associated with esophageal carcinogenesis we 1st tested the effects of small interfering RNA (siRNA) focusing on TIA1 on cell proliferation. (mdm-inhibitors.com)
By silencing endogenous TIA1 using three different siRNAs cell proliferation was significantly suppressed in KYSE140 KYSE180 and TE4 cells. (mdm-inhibitors.com)
Number 3 Effects of TIA1 knockdown on cell proliferation We next launched a TIA1[v1]- or TIA1[v2]-expressing retrovirus into KYSE190 and KYSE2270 cells expressing relatively lower levels of endogenous TIA1 to determine the effects of exogenously indicated TIA1 isoforms. (mdm-inhibitors.com)
Signaling pathways that regulate cell survival and proliferation are altered within the operation of carcinogenesis. (cetp-signal.com)
This signal transduction cascade regulates fundamental cellular processes which includes cell proliferation and survival, differentiation, Romidepsin and apoptosis. (cetp-signal.com)
Maintenance of cell survival and growth is achieved in component by means of the steady progression of cell cycle and consequent proliferation. (cetp-signal.com)
All elements during the Ras Raf Mek Erk cascade are actually shown to become associated with cell cycle progression, cell survival and proliferation. (cetp-signal.com)
Our recent examine showed that maintenance of protein tyrosine phosphorylation by PTP inhibition was connected with increased cell proliferation, clonogenic survival, and mutagenesis following just one Cr publicity in human lung fibroblasts. (cetp-signal.com)
The current study demonstrated that PD markedly inhibited PAC cell proliferation and triggered their apoptosis. (biomedcentral.com)
Their antitumor activities include inhibition of tumor cell proliferation, induction of tumor cell apoptosis and tumor angiogenesis, modulation of host immune responses, anti-inflammatory and anti-oxidative properties, and protective autophagy. (baituxiao.com)
Astragalus polysaccharides (APS) have been shown to regulate the immune function by enhancing the immune organ index, promoting the proliferation of immune cells, stimulating the release of cytokines, and affecting the secretion of immunoglobulin (Ig) and conduction of immune signals. (baituxiao.com)
The changes in the cell cycle, including cell proliferation and apoptosis, were determined. (lifeseasons.com)
Binding of one molecule of eribulin to two microtubules can inhibit cell proliferation by 50%, and such binding is reversible. (shu.edu)
ZKSCAN5-Regulated VEGFC Promotes the Proliferation, Migration, and Tube Formation of HLECs Cancer cell-secreted VEGFC markedly enhanced the proliferation and migration of lymphocyte endothelial cells. (bms-911543.com)
Because ZKSCAN5 improved the secretion of VEGFC by breast cancer cells, the effects of the conditioned medium on HLEC proliferation and migration were investigated in ZKSCAN5 knockdown stable cell lines. (bms-911543.com)
The ZKSCAN5 knockdown ZR75-1 or MDA-MB-231 cell-conditioned medium decreased HLEC proliferation. (bms-911543.com)
The conditioned medium from these cells re-expressing ZKSCAN5 could rescue these effects ( Figures?2A, B ). A similar tendency was also detected in HLEC migration analysis ( Figures?2C, D ). Open in a separate window Figure?2 VEGFC secreted by cancer cells, under the influence of ZKSCAN5, regulates HLEC proliferation, migration, and tube formation. (bms-911543.com)
A, B) Cell proliferation and colony formation assays in HLECs cultured in conditioned medium come from ZR75-1 or MDA-MB-231 cells stably infected with lentivirus carrying ZKSCAN5 shRNA or ZKSCAN5 shRNA plus ZKSCAN5-R. The representative Western blot displays the expression of ZKSCAN5. (bms-911543.com)

SFN induces cell cycle arrest and apoptosis in cancer cells, inhibits tubulin polymerization, activates checkpoint 2 kinase, and inhibits histone deacetylase activity. (sproutnet.com)

G1 Phase Cell Cycle Checkpoints" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
This graph shows the total number of publications written about "G1 Phase Cell Cycle Checkpoints" by people in this website by year, and whether "G1 Phase Cell Cycle Checkpoints" was a major or minor topic of these publications. (umassmed.edu)
Below are the most recent publications written about "G1 Phase Cell Cycle Checkpoints" by people in Profiles. (umassmed.edu)

Under this cascade, failure to synapse will maintain the gene expression from sex chromosomes and some products may inhibit cell cycle progression. (wikipedia.org)
CHEK2 regulates cell cycle progression and spindle assembly during mouse oocyte maturation and early embryo development. (wikipedia.org)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Checkpoint control kinases is a term used to describe a group of enzymes that regulate progression of a cell through the cell cycle. (tocris.com)
Regulatory signaling systems that control the progression of the CELL CYCLE through the G1 PHASE and allow transition to S PHASE when the cells are ready to undergo DNA REPLICATION. (umassmed.edu)
Further analysis with GenomeLab GeXP genetic system on brain tissues showed that the expression of most xenobiotic metabolism, cell progression, tumour suppressor, DNA damage and inflammation genes was maintained at normal level. (upm.edu.my)
12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
In the present study, we provide evidence that this aberrantly hyperphosphorylated protein is p34(cat2) (cdc2) a key regulator of cell-cycle progression. (uaeu.ac.ae)
These findings provide evidence that, in mature T lymphocytes, cell-cycle progression through the G 2 -M check point requires expression of the Src-family protein tyrosine kinase, Lck. (uaeu.ac.ae)
Hypoxia is considered a critical contributor to renal cell carcinoma progression, including invasion and metastasis. (biomedcentral.com)
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. (affbiotech.com)

A significant inhibition on cell growth, induction of apoptosis and cell cycle arrest were observed in 786-O and 769-P cells after kaempferol treatment compared with the control group. (nih.gov)
Moreover, the results clearly showed that kaempferol causes a strong inhibition of the activation of the EGFR/p38 signaling pathways, upregulation of p21 expression and downregulation of cyclin B1 expression in human RCC cells, together with activation of PARP cleavages, induction of apoptotic death and inhibition of cell growth. (nih.gov)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
To date, various mechanisms have been suggested to explain the powerful tumor-suppressive effect of p53, including the induction of cell cycle arrest, senescence, and apoptosis. (nature.com)
Moreover, ethanolic extracts of I'm-Yunity-Too exhibited more potent activity compared to its aqueous extracts with regard to suppression of the growth and induction of apoptosis, as assayed by the more pronounced downregulation of phosphorylation of Rb and increased cleavage of poly(ADP‑ribose) polymerase (PARP) from its native 112-kDa form to the inactive 89-kDa product. (spandidos-publications.com)
To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
Annexin-V assay has been used to detect the induction of apoptosis. (phcogj.com)
An early apoptosis induction in HepG2 cells was observed by annexin V assay, which clearly exhibited significantly increased early and late apoptosis phases both at 24 and 48 h. (phcogj.com)
A significantly increasing pattern of hypodiploid phases of cells also been observed, which confirm the apoptosis induction again. (phcogj.com)
These results suggest that TIA1 silencing in ESCC cells contributes to cell cycle arrest in the G1-S checkpoint and the induction of apoptosis. (mdm-inhibitors.com)
Through this mechanism, RPS27L induction by p53 facilitates p21-mediated cell cycle arrest and protects against DNA damage-induced apoptosis. (figshare.com)
β-peltatin initially arrested PAC cells at G2/M phase, followed by apoptosis induction. (biomedcentral.com)

MTT assay and colony formation assay were used to study cell growth, and flow cytometry was used to study apoptosis and cell cycles in different RCC cells treated with various doses of kaempferol. (nih.gov)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
The involvement of cell cycle check point arrest has been performed using flow cytometry analysis. (phcogj.com)
The cell cycle distribution was analyzed through PI staining and flow cytometry analysis, while apoptotic cells were measured by double staining with Annexin V-FITC and PI. (biomedcentral.com)

These DNA breaks must be repaired before metaphase I. and these DSBs must be repaired before metaphase I. The cell monitor these DSBs via ATM pathway, in which Cdc25 is suppressed when DSB lesion is detected. (wikipedia.org)
This pathway is the same as classical DNA damage response and is the part we know the best in meiotic recombination checkpoint. (wikipedia.org)
The DSB-independent pathway was proposed when people studied spo11 mutant cells in some species and found that these Spo11 cells could not process to metaphase I even in the absence of DSB. (wikipedia.org)
Bufalin induces G0/G1 phase arrest through inhibiting the levels of cyclin D, cyclin E, CDK2 and CDK4, and triggers apoptosis via mitochondrial signaling pathway in T24 human bladder cancer cells. (umassmed.edu)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
By using antisense RNA, Lck-deficient transfectants of a T helper 2 (Th2) clone have been derived and shown to have a qualitative defect in the T cell receptor signaling pathway. (uaeu.ac.ae)
Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
The Ras Raf Mek Erk pathway interacts with other mitogenic pathways to find out cell fate after extracellular stimuli. (cetp-signal.com)

During meiotic recombination in human and mouse, CHEK1 protein kinase is important for integrating DNA damage repair with cell cycle arrest. (wikipedia.org)
The oncogenic kinase PLK1 controls cell cycle and growth and is a cancer therapeutic target. (childrenscolorado.org)
Polo-like kinase-1 (PLK1) is highly expressed in rapidly dividing normal cells and is overexpressed in many types of cancer, including Group 3 medulloblastoma, and is associated with poor prognosis. (childrenscolorado.org)
EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
Checkpoint kinase 2 (CHEK2), a cell cycle checkpoint regulator gene, codes for a kinase protein activated in response to radiation and other agents that cause breaks in the DNA. (termedia.pl)
Treating SCCHN cell lines with a pan-Aurora kinase inhibitor resulted in defective cytokinesis, polyploidy and apoptosis, which was effective irrespective of the EGFR status. (oncotarget.com)

Selinexor inhibits XPO1, resulting in the accumulation of TSPs and other key mediators in the nucleus, restoring cell-cycle checkpoints and inducing growth arrest and apoptosis in malignant cells. (karyopharm.com)

These inhibitors inhibit NHEJ-catalyzed DSB repair and sensitize in vitro cancer cells to DSB-inducing agents. (termedia.pl)
PARP inhibitors (PARPis) display single-agent anticancer activity in small cell lung cancer (SCLC) and other neuroendocrine tumors independent of BRCA1/2 mutations. (bvsalud.org)
Blockade of CHK2 activation by CHK2 inhibitors protects cells from baicalein-mediated G2/M cell cycle arrest. (tku.edu.tw)

Generally speaking, the cell cycle regulation of meiosis is similar to that of mitosis. (wikipedia.org)
In normal cells, each stage of the cell cycle is tightly regulated, however in cancer cells many genes and proteins that are involved in the regulation of the cell cycle are mutated or over expressed. (tocris.com)
Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)
The overexpression of XPO1 is one mechanism by which neoplastic cells inactivate TSPs and thereby circumvent cell-cycle regulation, genome survey and apoptosis. (karyopharm.com)

This event is triggered by the activation of M-Cdk in late prophase I. Then the spindle assembly checkpoint examines the attachment of microtubules at kinetochores, followed by initiation of metaphase I by APCCdc20. (wikipedia.org)

Dysfunctional CHK2 impairs DNA damage-induced checkpoints, reduces apoptosis, and confers resistance to chemotherapeutic drugs and radiation therapy in ovarian cancer cells. (tku.edu.tw)
Depletion of RPS27L resulted in deficiency in DNA damage checkpoints, leading to conversion of DNA damage-induced p53 response from cell cycle arrest to apoptosis. (figshare.com)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
Upon publicity to a genotoxin cells undergo apoptosis, development arrest, and cell cycle checkpoint arrest according to the extent on the damage. (cetp-signal.com)
Using a panel of Kras-driven, p53 restorable cell lines derived from genetically engineered mouse models of lung adenocarcinoma and sarcoma (both of which undergo cell cycle arrest upon p53 restoration), as well as lymphoma (which instead undergo apoptosis), we show that the level of mitochondrial apoptotic priming is a critical determinant of p53 reactivation outcome. (nih.gov)

However, the expressions of a few xenobiotic metabolism, cell cycle arrest and apoptosis genes were up-regulated by 5 % ethanol injection. (upm.edu.my)
Several genetic alterations have been described in SCCHN, including mutations in the p53 tumor suppressor gene and mutations in genes that encode cell cycle proteins such as p16 and cyclin D1. (oncotarget.com)
A) Luciferase reporter genes were determined in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with Glutaminase-IN-1 different concentrations of reporter and myc-ZKSCAN5. (bms-911543.com)

Wu X, Song M, Qiu P, Li F, Wang M, Zheng J, Wang Q, Xu F, Xiao H. A metabolite of nobiletin, 4'-demethylnobiletin and atorvastatin synergistically inhibits human colon cancer cell growth by inducing G0/G1 cell cycle arrest and apoptosis. (umassmed.edu)
Here, the results show that baicalein (5,6,7-trihydroxyflavone) treatment inhibits the growth of highly invasive ovarian cancer cells, and that baicalein-induced growth inhibition is mediated by the cell cycle arrest in the G2/M phase. (tku.edu.tw)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
Although RPS27L mRNA levels were consistently induced after diverse p53 activating signals, its change in protein level was stimuli-dependent: it was up-regulated when cells were arrested in response to DNA-damaging agents Adriamycin or VP16 but was down-regulated when cells underwent apoptosis in response to antimetabolite agent 5-fluorouracil. (figshare.com)
Cells with high initial priming (e.g., lymphomas) lacked sufficient reserve antiapoptotic capacity and underwent apoptosis after p53 restoration. (nih.gov)

Checkpoint control kinases function to halt the cell cycle at these critical points. (tocris.com)
Tocris offers the following scientific literature for Checkpoint Control Kinases to showcase our products. (tocris.com)

Our results demonstrate that combined targeting of EGFR and Aurora kinases represents a therapeutic means to activate cell cycle checkpoints and apoptosis in SCCHN. (oncotarget.com)

Colony formation assays exposed that TIA1a-expressing cells created more colonies than control and TIA1b-expressing. (mdm-inhibitors.com)
p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)
VEGF-D (E, F) Tube formation assays for HLECs cultured in the conditioned medium from ZR75-1 or MDA-MB-231 cells, which were stably infected as in (A). All values shown are the mean SD of triplicate measurements and were repeated three times with analogous. (bms-911543.com)

Grape-related anti-tumoral activity encompasses a wide array of biological mechanisms and cellular targets, eventually leading to inhibition of cell growth and to enhanced apoptosis in several cancer cell lines, including lung, colon, breast, bladder, leukemia and prostate tumors. (oatext.com)

A549 xenografts by inducing apoptosis, as confirmed by TUNEL assay. (hindawi.com)
Cell viability was determined using Cell Counting Kit-8 assay. (biomedcentral.com)

Once activated, p53 coordinates multiple downstream pathways, thereby maintaining the homeostasis of the host cell or organism (if the stress is mild, transient, and repairable) or eliminating damaged cells (if the stress is acute, prolonged, and difficult to resist). (nature.com)
Pope PA, Pryciak PM. Functional overlap among distinct G1/S inhibitory pathways allows robust G1 arrest by yeast mating pheromones. (umassmed.edu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In vitro, don quai triggered both the intrinsic and extrinsic pathways for apoptosis. (lifeseasons.com)

Silencing of the EBF3 locus was observed in brain, colorectal, breast, liver, and bone tumor cell lines and its reactivation was achieved on treatment with 5-aza-2′-deoxycytidine and trichostatin A in a significant portion of these tumor cells. (aacrjournals.org)
5) increasing the intrinsic radioresistance of normal cells through ketone bodies but decreasing that of tumor cells by targeting glycolysis. (springer.com)
Similarly both non-tumor and tumor cells of main ESCC predominantly indicated mRNA and the mRNA manifestation levels in tumors were higher than in ANGPT1 those in combined non-tumor cells in 3/6 (50%) of ESCC instances whose RNA was available (Supplementary Number S3B). (mdm-inhibitors.com)
In vivo, rat RG2 glioblastoma and human brain glioblastoma tumor cells were injected into the skin or into the eye cavity and treated with don quai. (lifeseasons.com)

In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These compounds decreased cell viability more significantly in colon cancer cells than in normal colon cells dose-dependently. (lifeseasons.com)

Cell cycle and DNA replication are top signaling hubs for medulloblastoma cell growth. (childrenscolorado.org)
Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress. (umassmed.edu)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
TG 003 signaling dynamics and to induce cell cycle arrest and apoptosis in ERK-dependent cancers. (cell-signaling-pathways.com)

The first major regulatory transition occurs in late G1, when the start of meiotic cycle is activated by Ime1 instead of Cln3/Cdk1 in mitosis. (wikipedia.org)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
We have already written a detailed blogpost about the characteristic phases of mitosis but as a reminder, metaphase describes the stage when the cell's chromosomes align along an invisible line in the center of the cell called the metaphasic plate. (nanolive.ch)
Cells entering mitosis in the presence of Taxol form abnormal spindles that contain additional spindle poles. (nanolive.ch)
Similar to these drugs, eribulin is a microtubule inhibitor that induces apoptosis of cancer cells by stopping mitosis in the G2/M phase of the cell cycle . (shu.edu)

Don quai also increased the proteins that are involved in apoptosis which increases programmed cancer cell death. (lifeseasons.com)

These cells possess both differentiation plasticity (stemness) and tissue supportive functions (stromalness) that can coexist and overlap, with differences depending on tissue source, donor characteristics, culture conditions and delivery strategies, leading to alternative best fittings for the term "stem" or "stromal" [ 7 ] . (encyclopedia.pub)

Kaempferol has been shown to inhibit cell growth, induce apoptosis and cell cycle arrest in several tumors, but not in renal cell carcinoma (RCC). (nih.gov)
Overexpression of the solute carrier family 14 member 1 gene could abolish hypoxia-induced invasion, reduce the migration of A498 cells, inhibit the hypoxia-induced release of inflammatory cytokines, and arrest the cell cycle at the G1/S checkpoint. (biomedcentral.com)

The human renal cell carcinoma cell line A498 under hypoxia/normoxia was used as an experimental model in vitro and the biological characteristics and mitochondrial function of the cells were assessed. (biomedcentral.com)
Mitochondrial apoptotic priming is a key determinant of cell fate upon p53 restoration. (nih.gov)
We hypothesized that high mitochondrial priming prior to p53 reactivation would lead to apoptosis, while low priming would lead to survival and cell cycle arrest. (nih.gov)

When primed by inhibition of their antiapoptotic proteins using genetic (BCL-2 or BCL-XL deletion or BAD overexpression) or pharmacologic (navitoclax) means, apoptosis resulted upon p53 restoration in vitro and in vivo. (nih.gov)

Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
EESR-induced apoptosis was associated with the upregulation of p53, a death receptor Fas, and a pro-apoptotic protein Bax and the activation of caspase 3, 8, and 9, resulting in the degradation of PARP. (jcpjournal.org)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
A striking feature observed only in Lck- deficient T cells was the presence of a constitutively tyrosine- phosphorylated 32-kDa protein. (uaeu.ac.ae)
Immunoblotting with and without dephosphorylation was used to define the protein isolated from breast cancer cells. (biomedcentral.com)
Increased hRad9 protein was observed in breast cancer cells nucleus compared to non-tumor epithelium. (biomedcentral.com)
To further examine the role of hRad9 in breast cancer cells, we now report on the histologic expression of the hRad9 protein and its different molecular forms in primary breast cancer and normal tissues. (biomedcentral.com)
The p53 (phospho-)protein (p53), 53 kDa, is expressed in the nuclei of all normal cells, but usually not immunohistochemically detectable due to a very short half-life (10-20 min. (nordiqc.org)
Normal (wild type - WT) p53 is accumulated in damages cells, e.g., in virus infections, where the protein is bound (e.g., to protein E6 of human papilloma virus) and prevented from break down. (nordiqc.org)
Also the over-expression of mdm2 protein arrests the p53 turnover. (nordiqc.org)
Another study found that extracts of Astragalus membranaceus overcome tumor immune tolerance by inhibition of tumor programmed cell death protein ligand-1 expression. (baituxiao.com)
Chang HL, Kuo YH, Wu LH, Chang CM, Cheng KJ, Tyan YC, Lee CH. The extracts of Astragalus membranaceus overcome tumor immune tolerance by inhibition of tumor programmed cell death protein ligand-1 expression. (baituxiao.com)
D) Cytoplasmic and nuclear ZKSCAN5 protein levels in two types of breast cancer cell lines, ZR75-1 and MDA-MB-231. (bms-911543.com)
The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. (affbiotech.com)

Among these unconventional activities, an iron-dependent form of non-apoptotic cell death, termed ferroptosis, attracts great interest. (nature.com)
By assaying their anti-proliferative and anti-apoptotic effects using human promyelocytic HL-60 cells, we found that the ethanolic extract of I'm-Yunity-Too was more active in inducing cell death compared to I'm-Yunity, based on measured changes in the expression of caspase 3 and Bax. (spandidos-publications.com)
In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
Eribulin exerts its effects via a tubulin-based antimitotic mechanism leading to G2/M cell-cycle block, disruption of mitotic spindles, and, ultimately, apoptotic cell death after prolonged mitotic blockage. (shu.edu)

The current study found that ST has the capacity to induce cell toxicity in human liver cells. (phcogj.com)

A) Cell viability at 72 hours and (B) 6 days post-siCKAP5 treatment as measured by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT). (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)

In the present study, we investigated the effects of kaempferol and the underlying mechanism(s) on the cell growth of RCC cells. (nih.gov)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
The novel PLK1 inhibitor, onvansertib, is the first available for oral administration and has shown tumor growth inhibition in hematologic tumors, osteosarcoma, ovarian carcinoma, breast cancer and colon adenocarcinoma cells. (childrenscolorado.org)
Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
Importantly, however, this hypersusceptibility was largely reversed in the presence of T cell growth factors. (uaeu.ac.ae)
Exportin 1 (XPO1) shuttles a wide variety of proteins, including TSPs and growth regulating proteins, from the cell nucleus to the cytoplasm. (karyopharm.com)
Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)
Finding of hyperphosphorylated forms of hRad9 in the nucleus of cancer cells is in keeping with its function in ameliorating DNA instability, whereby it inadvertently assists tumor growth. (biomedcentral.com)
These genetic aberrations may cause loss of growth inhibition in normally quiescent cells and result in carcinogenesis [ 5 ]. (biomedcentral.com)
However, in established cancer cells, such DNA repair system may prevent further DNA damage in their progeny and decrease in apoptosis resulting in enhancement of tumor growth. (biomedcentral.com)
By binding to DNA, the normal p53 negatively regulates cell growth and division. (nordiqc.org)
All the results suggest that baicalein has another novel growth inhibitory effect on highly invasive ovarian cancer cells, which is partly related to G2/M cell cycle arrest through the ROS-mediated DNA breakage damage and CHK2 activation. (tku.edu.tw)
Animal study confirmed that β-peltatin significantly suppressed the growth of subcutaneously-implanted BxPC-3 cell xenografts. (biomedcentral.com)
In summary, the present study demonstrated that PT and ATST produce a powerful synergistic interaction in suppressing colon cancer cell growth. (epiphanyasd.com)

From the in vivo study, results showed that treatment of 300 mg/kg of vanillin by intra-peritoneal injection caused the rats to be unconscious without exerting any negative effect on blood cells, kidney and liver. (upm.edu.my)
The in vivo effects of β-peltatin and podophyllotoxin were evaluated on a subcutaneously-xenografted BxPC-3 cell nude mice model. (biomedcentral.com)
Due to the low abundance of MSCs in human adult tissues (about 1/10 6 cells in adult bone marrow and 1/10 3-4 cells in adipose tissue and umbilical cord) [ 16 ] , frequently ex-vivo expansion precedes therapeutic administration, to obtain a clinically relevant number of cells. (encyclopedia.pub)

These checkpoints may stop the cell cycle after DNA damage, loss of DNA replication or disruption of the mitotic spindle, in order for repair processes to take place. (tocris.com)
Normally, structural damage of DNA by endogenous and environmental agents is followed by replication checkpoint arrest at the G2/M transition in order to allow for repair before proceeding in the cell cycle. (biomedcentral.com)

In pediatric solid tumors and brain tumors, PLK1 inhibition leads to apoptosis in vitro and in tumor xenografts. (childrenscolorado.org)
Reactivation of p53 in established tumors typically results in one of two cell fates, cell cycle arrest or apoptosis, but it remains unclear how this cell fate is determined. (nih.gov)

It also highlights strategies for enhancing replicative stress in cancer cells to force mitotic catastrophe and cell death. (tocris.com)
In conclusion, vanillin could induce cytotoxic effects on colorectal cancer cells. (upm.edu.my)
Cancer cells can grow unusually via limitless number of cell division and can avoid apoptosis. (jcpjournal.org)
DNA repair targeted therapy, which kills cancer cells preventing DNA repair [1-3]. (termedia.pl)
In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
Among the target compounds, 8h possessed nano-molar IC50 values toward the tested cancer cells and HDAC subtypes, which was more potent than the HDAC inhibitor SAHA (vorinostat). (bvsalud.org)
Baicalein Induces G2/M Cell Cycle Arrest Associated with ROS Generation and CHK2 Activation in Highly Invasive Human Ovarian Cancer Cells. (tku.edu.tw)
The cytotoxicity and anti-proliferative effects of these phthalides and their cooperation on colon cancer cells were tested. (lifeseasons.com)
Researchers compared the results of both human colon cancer cells and normal colon cells. (lifeseasons.com)
Statistical Analyses Statistical significance was assessed by using the two-tailed Students in breast cancer cells. (bms-911543.com)
B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
The colon gene expression analysis showed that vanillin could enhance recombinational repair and mismatch repair, arrest cell at cell cycle checkpoints, increase the expression of tumour suppressor gene, colorectal cancer biomarker and proto-oncogene. (upm.edu.my)
In addition, it has to be emphasized, that stromal cells, lymphocytes and endothelial cells in the clinical samples are essential as internal positive tissue controls especially for carcinomas with TP53 mutations causing absence and loss of p53 expression in the tumour cells. (nordiqc.org)
For tumour estimation, nude mice were inoculated subcutaneously with 1 107 ZR75-1 cells with different constructs on the right side. (bms-911543.com)

It is a cytoskeletal drug, which binds to a specific site on β-tubulin [2], a major component of the microtubules that form the structure and shape of eukaryotic cells. (nanolive.ch)

The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)

Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
Collectively, results presented in this study demonstrated that the ST, which is used as a euphoritic substance of abuse also, has significant level of toxicity in human cells. (phcogj.com)

IF/ICC: For immunofluorescence detection of cell samples. (affbiotech.com)

The special chromosome separation in meiosis, homologous chromosomes separation in meiosis I and chromatids separation in meiosis II, requires special tension between homologous chromatids and non-homologous chromatids for distinguishing microtubule attachment and it relies on the programmed DNA double strand break (DSB) and repair in prophase I. Therefore meiotic recombination checkpoint can be a kind of DNA damage response at specific time spot. (wikipedia.org)
Therefore the unsynapsed chromosomes in Spo11 cells can be a target of checkpoint. (wikipedia.org)
For a cell to progress through the cycle and replicate, it must pass through checkpoints between phases to ensure that DNA is replicated correctly and that chromosomes segregate. (tocris.com)
Only when all chromosomes are aligned, and every kinetochore is properly attached to a bundle of microtubules, can the cell proceed to enter anaphase. (nanolive.ch)
Rather than mounting a long-term mitotic arrest, these cells enter anaphase and divide their chromosomes in multiple directions, resulting in a multi-nucleated cell. (nanolive.ch)

Although the conventional activities of p53 such as cell cycle arrest, senescence, and apoptosis are well accepted as the major checkpoints in stress responses, accumulating evidence implicates the importance of other tumor suppression mechanisms. (nature.com)
Is p53-dependent ferroptosis sufficient for tumor suppression in the absence of cell cycle arrest, senescence, and apoptosis? (nature.com)
MSC use frequently requires in vitro expansion, thus exposing cells to replicative senescence. (encyclopedia.pub)

In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)

If cells containing damaged DNA were to divide, the errors would be transmitted to daughter cells, generating genomic instability and resulting in tumorigenesis or apoptosis . (tocris.com)
Mutated p53 shows impaired DNA-binding and, consequently, release from cell cycle checkpoint control following DNA damage, thereby increasing genomic instability and promoting gene amplification. (nordiqc.org)

Taxol works by stabilizing the microtubule polymer, preventing the disassembly of microtubules during the fourth phase of cell division, metaphase [3,4]. (nanolive.ch)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Hypoxia was found to induce A498 cell invasion, migration, and the release of inflammatory cytokines, while repressing human solute carrier family 14 member 1 gene expression. (biomedcentral.com)

In addition to STS, selinexor has received Orphan Drug Designation from the U.S. FDA for use in the treatment of multiple myeloma (MM), acute myeloid leukemia (AML) and diffuse large B-cell lymphoma (DLBCL). (karyopharm.com)

Taxol disrupts normal microtubule dynamics, activating the spindle checkpoint, which triggers mitotic arrest and/or cell death via apoptosis [3,4]. (nanolive.ch)
Present findings indicate that T44Bf-mediated antiproliferative effects are associated with a reversible chronic mitotic arrest caused by defects in chromosome alignment, followed by induced programmed cell death. (tmu.edu.tw)

Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)
In appendix, dispersed epithelial cells in the basal parts of the crypts must show a weak to moderate nuclear staining reaction, while the luminal epithelial cells must be negative. (nordiqc.org)
Renal cell carcinoma (RCC) originates from either the cancerous renal cortex or renal tubular epithelial cells, and is the second most lethal urological malignancy, accounting for 90-95% of kidney neoplasms. (biomedcentral.com)

this kind of a selective development benefit might possibly allow to the emergence of transformed cells. (cetp-signal.com)

Whereas damage signals of cells activate p53 via CHK2 to repair damaged DNA, leading to cell cycle arrest. (jcpjournal.org)
Baicalein-induced G2/M phase arrest is associated with an increased reactive oxygen species (ROS) production, DNA damage, and CHK2 upregulation and activation. (tku.edu.tw)

RNA was extracted from colon for DNA repair, apoptosis, cell cycle, antiinflammation, proto-oncogene, colorectal cancer biomarker and tumor suppressor gene expressions study. (upm.edu.my)

Anatomy23

Organisms5

Diseases10

Chemicals and Drugs121

Analytical, Diagnostic and Therapeutic Techniques and Equipment18

Phenomena and Processes85

Disciplines and Occupations1

Information Science3

Health Care3

Proliferation32

Induces cell cycle arrest and apoptosis1

Phase Cell Cycle Checkpoints3

Progression11

Induction13

Flow cytometry4

Pathway9

Kinase7

Malignant cells1

Inhibitors3

Regulation4

Spindle assembly checkpoint1

Damage-induced2

Undergo3

Genes3

Inhibits2

Underwent3

Control Kinases2

Kinases1

Assays3

Inhibition of cell growth1

Assay2

Pathways4

Tumor cells4

Dose-dependently2

Signaling4

Mitosis8

Proteins that are involved1

Differentiation1

Inhibit2

Mitochondrial3

Vitro and In Vivo1

Protein15

Apoptotic5

Induce cell1

Viability2

Growth19

Vivo3

Replication2

Tumors2

Cancer cells11

Tumour4

Tubulin1

Anticancer effects2

Collectively2

Immunofluorescence1

Chromosomes5

Senescence3

Antitumor effects2

Genomic instability2

Microtubules1

Migration2

Acute1

Mitotic arrest2

Epithelial4

Selective1

Chk22

Colorectal cancer1