Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus. (wikipedia.org)
It was found to inhibit in vitro CDP-DG:inositol transferase activity of the A431 cell membrane, the IC 50 being about 0.02 μg/ml. (elsevierpure.com)
Therefore, inhibition of PtdIns turnover by inostamycin must be due to the inhibition of CDP-DG:inositol transferase. (elsevierpure.com)
Thus, inostamycin is a novel inhibitor of CDP-DG:inositol transferase. (elsevierpure.com)
Phosphatidylinositol6
Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of multiple G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. (wikipedia.org)
Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of phosphatidylinositol. (wikipedia.org)
A decrease of PIP2 levels, in particular through mutations in Phosphatidylinositol synthase (Pis) and other members of the phosphoinositide pathway, leads to the aberrant accumulation of BM components at the apical side of the cell without primarily affecting the distribution of apical and basolateral polarity proteins. (princeton.edu)
Phosphatidylinositol can be phosphorylated by a number of different kinases that place the phosphate moiety on positions 4 and 5 of the inositol ring, although position 3 can also be phosphorylated by a specific kinase. (ymdb.ca)
Phosphatidylinositol and the phosphatidylinositol phosphates are the main source of diacylglycerols that serve as signaling molecules, via the action of phospholipase C enzymes. (ymdb.ca)
Inostamycin, a novel microbial secondary metabolite, inhibited [ 3 H]inositol and 32 P 1 incorporation into phosphatidylinositol (PtdIns) induced by epidermal growth factor (EGF) in cultured A431 cells, the IC 50 being 0.5 μg/ml, without inhibiting macromolecular synthesis. (elsevierpure.com)
Inhibition1
Raf inhibition has become the target for new anti-metastatic cancer drugs as they inhibit the MAPK cascade and reduce cell proliferation. (cloudfront.net)
Enzyme2
CDP-diacylglycerol-inositol 3-phosphatidyltransferase is an enzyme that in humans is encoded by the CDIPT gene. (wikipedia.org)
The enzyme functions to stimulate growth of cells. (cloudfront.net)
Synthesis1
The synthesis of PI involves CDP-activated 1,2-diacylglycerol condensation with myo-inositol. (ymdb.ca)
Cellular2
The drug inhibited cellular inositol phosphate formation only when it was added at the same time as labeled inositol. (elsevierpure.com)
respond to extracellular stimuli (mitogens) and regulate various cellular activities, such as gene expression, mitosis, differentiation, and cell survival/apoptosis. (cloudfront.net)
Receptors1
Human homologs of the AKT8 oncogenic protein were identified in 1987.By 1995 it had been found that Akt kinases function as mitogen-activated kinases downstream from cell surface receptors that activate phosphoinositide 3-kinase . (cloudfront.net)
Regulation1
Serine/threonine kinases play a role in the regulation of cell proliferation, programmed cell death ( apoptosis ), cell differentiation, and embryonic development. (cloudfront.net)
Structure1
The basement membrane (BM), a specialized sheet of the extracellular matrix contacting the basal side of epithelial tissues, plays an important role in the control of the polarized structure of epithelial cells. (princeton.edu)
Form1
In most organisms, the stereochemical form of the last is myo-D-inositol (with one axial hydroxyl in position 2 with the remainder equatorial. (ymdb.ca)
Protein8
Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of multiple G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. (wikipedia.org)
Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-I family, is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgi apparatus. (wikipedia.org)
HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)
HN - 2006(1998) MH - Actin-Related Protein 2-3 Complex UI - D051376 MN - D5.750.78.730.246 MN - D12.776.220.525.246 MS - A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. (nih.gov)
Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments. (nih.gov)
HN - 2006 BX - Arp2-3 Complex MH - Actin-Related Protein 3 UI - D051378 MN - D5.750.78.730.246.750 MN - D12.776.220.525.246.750 MS - A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. (nih.gov)
Human homologs of the AKT8 oncogenic protein were identified in 1987.By 1995 it had been found that Akt kinases function as mitogen-activated kinases downstream from cell surface receptors that activate phosphoinositide 3-kinase . (cloudfront.net)
CDIPT1
CDP-diacylglycerol-inositol 3-phosphatidyltransferase is an enzyme that in humans is encoded by the CDIPT gene. (wikipedia.org)
Involved in the biosynthesis1
Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesis of phosphatidylinositol. (wikipedia.org)
Biosynthesis2
CDP-diacylglycerol (CDP-DG) is an important branchpoint intermediate in eukaryotic phospholipid biosynthesis and could be a key regulatory molecule in phospholipid metabolism. (hmdb.ca)
Catalyzes the biosynthesis of phosphatidylinositol (PtdIns) as well as PtdIns:inositol exchange reaction. (nih.gov)
Role1
Serine/threonine kinases play a role in the regulation of cell proliferation, programmed cell death ( apoptosis ), cell differentiation, and embryonic development. (cloudfront.net)
Gene1
respond to extracellular stimuli (mitogens) and regulate various cellular activities, such as gene expression, mitosis, differentiation, and cell survival/apoptosis. (cloudfront.net)
Reduce1
Raf inhibition has become the target for new anti-metastatic cancer drugs as they inhibit the MAPK cascade and reduce cell proliferation. (cloudfront.net)