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  • fetal
  • A first wave of hematopoiesis follows MHE colony emergence and is predominated by primitive erythropoiesis characterized by a brilliant red hemoglobinization, CD71/CD325a (glycophorin A) expression, and exclusively embryonic/fetal hemoglobin expression. (bloodjournal.org)
  • These new neurons arise from 'neural stem cells' in the fetal as well as the adult brain. (google.com)
  • culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of fibroblast growth factor that is supplied from a source other than just a fibroblast feeder layer. (google.com)
  • culturing the stem cells in a culture essentially free of mammalian fetal serum and in the presence of a growth factor capable of activating a fibroblast growth factor signaling receptor, wherein the growth factor is supplied from a source other than just a fibroblast feeder layer. (google.com)
  • mammalian
  • Pubmed ID: 21151123 In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. (jove.com)
  • However, the relative contributions of these Ca 2+ transporters during mammalian embryonic cardiogenesis remain controversial. (physiology.org)
  • proteins
  • During differentiation, cardiac-specific genes as well as proteins, receptors, and ion channels are expressed in a developmental continuum, which closely recapitulates the developmental pattern of early cardiogenesis. (ahajournals.org)
  • These features are maintained ex vivo in mouse ESCs (mESCs) using leukemia inhibitory factor (LIF), a cytokine capable of activating STAT signaling, in concert with either serum or bone morphogenic protein (BMP) to induce inhibitor-of-differentiation (Id) proteins ( 1 , - 3 ). (pubmedcentralcanada.ca)
  • Since GATA DNA-binding proteins play crucial roles in the initiation of cardiomyocyte differentiation, such a response could be ascribed to the presence of multiple GATA motifs in the enhancer sequence but not in the native MLC-2v promoter. (scirp.org)
  • In the past year, we figured out several mechanisms that prevent the efficient incorporation of nonnatural amino acids into proteins in stem cells. (ca.gov)
  • In the meantime, we developed another complementary approach in order to detect unknown proteins that interact with Nurr1 during the differentiation process of stem cells. (ca.gov)
  • Aquaporins are a family of water channel proteins that provide a major pathway for osmotically driven water transport through cell membranes. (biologists.org)
  • We have previously shown that p63, a member of the p53 family of nuclear proteins, is expressed in proliferative cytotrophoblast (CTB), precursors to terminally differentiated syncytiotrophoblast (STB) in chorionic villi and extravillous trophoblast (EVT) at the implantation site. (biologists.org)
  • apoptosis
  • The Rat Apoptosis EpiTect Methyl II Signature PCR Array profiles the promoter methylation status of a panel of 22 promoters of genes involved in apoptosis, or programmed cell death. (qiagen.com)
  • Flow cytometric cell cycle analysis revealed that AQP4 knockout increased the basal apoptosis and induced a G2-M arrest in ANSCs. (biologists.org)
  • Gene Expression
  • Gene expression analysis of differentiating hEBs revealed that initiation of hematopoiesis correlated with increased levels of SCL/TAL1, GATA1, GATA2, CD34, CD31, and the homeobox gene-regulating factor CDX4 These data indicate that hematopoietic differentiation of hESCs models the earliest events of embryonic and definitive hematopoiesis in a manner resembling human yolk sac development, thus providing a valuable tool for dissecting the earliest events in human HSPC genesis. (bloodjournal.org)
  • These cells manifest gene expression profiles that are distinct from those of proliferating mESCs. (pubmedcentralcanada.ca)
  • Here, we show that BMP4-treated hESCs differentiate into bona fide CTB by direct comparison with primary human placental tissues and isolated CTB through gene expression profiling. (biologists.org)
  • fibroblast
  • H1 [Wi-Cell] and hES-NCL1 [Newcastle University, United Kingdom] hES cells were then plated on the collagen IV using limbal fibroblast conditioned medium. (arvojournals.org)
  • These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. (google.com)
  • propagated in vitro
  • They can be propagated in vitro for long periods of time in an undifferentiated state and thus represent an attractive source of cells for developmental studies and for therapy of human diseases. (google.com)
  • tissues
  • The expression data suggest that miRNAs are important factors in differentiating tissues in adult organisms. (thermofisher.com)
  • however, embryonic stem (ES) cells do not normally generate tissues of the trophoblast, precluding normal generation of a viable entity. (ahajournals.org)
  • Oligonucleotide-based fluorescent in situ hybridization (FISH) coupled with high-resolution high-sensitivity microscopy allows the visualization of single RNA molecules within fixed cells and tissues as distinct foci. (biologists.org)
  • progeny
  • 7 - 17 Interestingly, in both mouse and humans, there is compelling evidence that both yolk sac- and AGM-derived HSPCs are the direct progeny of a bipotential hemogenic endothelial cell or hemangioblast. (bloodjournal.org)
  • Neural Stem
  • However, the non-invasive isolation and purification of significant numbers of neural stem cells from the brain remain challenging. (google.com)
  • Aquaporin-4 (AQP4), a key molecule for maintaining water and ion homeostasis in the central nervous system, is expressed in adult neural stem cells (ANSCs) as well as astrocytes. (biologists.org)
  • assay
  • A series of related indicators were examined by cell counting kit-8 (CCK-8) assay, quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blot, alkaline phosphatase staining, propidium iodide (PI) staining, Annexin V staining, competition growth assay, immunofluorescence, and chromatin immunoprecipitation (ChIP)-qPCR. (springer.com)
  • In addition to our expansive portfolio of assay ready stem cells, serum-free cell culture media , and 3D culture solutions , we offer custom engineered stem cell lines through our easy-to-use Cell Design Studio . (sigmaaldrich.com)
  • somatic
  • Controlling the expansion of somatic stem cells is a major factor hampering their study and use in therapy. (jove.com)