Cells by macrophages. Medical search. Frequent questions

Thus, there remains an unmet need for cost-effective methods for generating and/or differentiating primary cells into a HAM phenotype, particularly important for translational and clinical studies. (genengnews.com)
We developed cell culture conditions that mimic the lung alveolar environment in humans using lung lipids, that is, Infasurf (calfactant, natural bovine surfactant) and lung-associated cytokines (granulocyte macrophage colony-stimulating factor, transforming growth factor-β, and interleukin 10) that facilitate the conversion of blood-obtained monocytes to an AM-like (AML) phenotype and function in tissue culture. (genengnews.com)
A more tissue-centric view of these processes, claiming that the tissues define phenotype and function of resident and infiltrating immune cells to meet tissues needs during homeostasis and disease, seems provocative [ 4 , 5 ]. (hindawi.com)
For example, organs like the lung and the liver are exposed to pathogen components from the air or from the gut barrier, respectively, which explains the predominance of a macrophage phenotype that has a higher capacity for phagocytic clearance of pathogen components. (hindawi.com)
Tumor-associated macrophages (TAMs) polarized to the M2 phenotype promote tumor cell proliferation and are associated with a poor prognosis in patients with high grade glioma. (spandidos-publications.com)
OA significantly inhibited the expression of CD163, one of the phenotype markers of M2 macrophages, as well as suppressed the secretion of IL-10, one of the anti-inflammatory cytokines preferentially produced by M2 macrophages, thus suggesting that OA suppresses the M2 polarization of macrophages. (spandidos-publications.com)
Fujiwara Y, Komohara Y, Kudo R, Tsurushima K, Ohnishi K, Ikeda T and Takeya M: Oleanolic acid inhibits macrophage differentiation into the M2 phenotype and glioblastoma cell proliferation by suppressing the activation of STAT3. (spandidos-publications.com)
Here, we show that successful treatment of cervical carcinoma in mouse models with synthetic long peptide (SLP) vaccines induced influx of cytokine-producing CD8 T cells that strongly altered the numbers and phenotype of intratumoral macrophages. (aacrjournals.org)
Intestinal macrophages display a "tolerant" phenotype, poorly responsive to toll like receptor (TLR) ligands (vital to avoid triggering inflammatory responses while scavenging commensal bacteria) whilst maintaining a homeostatic environment via IL-10 and PGE 2 production ( 7 ). (frontiersin.org)
In the context of acute kidney injury, M-CSF has been implicated in promoting repair following injury, but also been described in an opposing role, driving proliferation of a pro-inflammatory macrophage phenotype. (wikipedia.org)
The results showed that lactic acid induced macrophage polarization towards an M2-like phenotype and orchestrated GPNMB secretion from macrophages . (bvsalud.org)

Using interleukin (IL)-33 receptor knockout mice and in vitro experiments, we show that basophils establish a lung-specific function imprinted by IL-33 and granulocyte-macrophage colony-stimulating factor (GM-CSF), characterized by unique signaling of cytokines and growth factors important for stromal, epithelial, and myeloid cell fates. (nih.gov)
In macrophage-colony stimulating factor (MCSF) generated macrophages, Sia + IgG induced increased production of numerous cytokines/ chemokines including IL-6, TNFα, CXCL1, and IL-10, and the expression of the macrophage surface marker CD163. (scirp.org)
D. Bruder, E. , O. Richards, J. , M. Michel, K. and Oaks, M. (2016) Differential Effects of Alternative Glycoforms of IgG on Human Monocytes and Macrophages: Sialylated IgG Induces Novel Expression Signatures of Cell Surface Markers, Cytokines, and Chemokines. (scirp.org)
There is evidence that 2D or 3D culturing conditions have an impact on the cells' secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. (biomedcentral.com)
Treatment of cells with NF-κB and MAPK inhibitors abolished the effect of the cytokines on CCL3 expression. (shu.ac.uk)
Through mechanisms that have not yet been well elucidated, this impairment in cytotoxic function leads to an excessive expansion and activation of cytotoxic cells, with hypersecretion of proinflammatory cytokines such as interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10, and macrophage-colony-stimulating factor (M-CSF). (medscape.com)
These cytokines are produced by activated T cells and histiocytes that infiltrate all tissue and lead to tissue necrosis and organ failure. (medscape.com)
The elevated levels of inflammatory cytokines and chemokines (e.g., interleukin [IL] 6, IL-8, macrophage inflammatory protein 1α and 1β, monocyte chemoattractant protein 1, and macrophage colony-stimulating factor) and immunomodulatory cytokines (e.g. (cdc.gov)
Here, we show that MM-derived macrophage migratory inhibitory factor (MIF) stimulates bone marrow stromal cells to produce the disease critical cytokines IL-6 and IL-8, prior to any cell-cell contact. (biomedcentral.com)

Sia + IgG induced increased expression of CD80 and dendritic cell immunoreceptor (DCIR) on monocytes, whereas the expression of HLA-DR was decreased. (scirp.org)
Our data extended prior observations of Sia + IgG on DC function and showed that Sia + IgG was able to differentially modulate multiple pathways in monocytes and macrophages. (scirp.org)
However, it is not clear how gut macrophages, relative to recruited blood monocytes and other myeloid cells, contribute to the intestinal inflammatory milieu, nor how macrophages and their monocyte precursors mediate recruitment of other immune cells to the inflamed intestine. (frontiersin.org)
In addition, mRNA expression profiles in human and murine colon samples, and in macrophages and monocytes from healthy and inflamed murine colons, were analysed by quantitative PCR (qPCR) and mRNA microarray. (frontiersin.org)
We show that the monocyte:macrophage balance is disrupted in colon inflammation to favour recruitment of CD14 + HLA-DR Int cells in humans, and Ly6C Hi monocytes in mice. (frontiersin.org)
Further, once within the colon and relative to other myeloid cells, monocytes represent the dominant local source of both IL-1β and TNF. (frontiersin.org)
It has recently been shown that murine intestinal macrophages are continually repopulated from circulating blood monocytes, identified by expression of the cell surface markers Ly6C, CCR2, and CD62L ( 6 ). (frontiersin.org)
However, the functions of mature intestinal macrophages and recruited monocytes are strikingly different. (frontiersin.org)
Results from murine studies ( 2 ) combined with the observation of radiolabelled blood monocytes in the inflamed intestinal mucosa of IBD patients ( 15 ), indicate that increased LP inflammatory mononuclear cells are a result of monocyte recruitment, rather than expansion of a tissue resident macrophage population. (frontiersin.org)
M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. (wikipedia.org)
M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. (wikipedia.org)
Immunomagnetically selected peripheral blood monocytes are cultured in RPMI 1640 Medium (Catalog #36750) + 10% FBS, M-CSF, and IL-4 for 5 days to generate macrophages. (stemcell.com)
Human peripheral blood monocytes were isolated and cultured for 7 days to obtain monocyte derived macrophages. (biomedcentral.com)
Parent cells in the lineage that gives rise to MONOCYTES and MACROPHAGES. (ouhsc.edu)
These cells include a subset of helper T lymphocytes, monocytes and macrophages. (cdc.gov)
Macrophages develop from a type of white blood cell called monocytes. (msdmanuals.com)
Monocytes become macrophages when they move from the bloodstream to the tissues. (msdmanuals.com)
There, over a period of about 8 hours, monocytes enlarge greatly and produce granules within themselves, becoming macrophages. (msdmanuals.com)

The present study examined the effects of Sia + IgG on human peripheral blood mononuclear cell (PBMC)-derived monocyte and macrophage surface marker expression and cytokine/chemokine secretion. (scirp.org)
We previously revealed that corosolic acid, a triterpenoid compound, inhibits the M2 polarization of human monocyte-derived macrophages (HMDM). (spandidos-publications.com)
Finally, our data reveal that, independent of inflammation, murine colon macrophages act as a major source of Ccl7 and Ccl8 chemokines that trigger further recruitment of their pro-inflammatory monocyte precursors. (frontiersin.org)
Our work suggests that strategies targeting macrophage-mediated monocyte recruitment may represent a promising approach for limiting the chronic inflammation that characterises IBD. (frontiersin.org)
The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. (wikipedia.org)
SAP binds to late apoptotic cells and is involved in the phagocytosis of these cells by human monocyte derived macrophages. (biomedcentral.com)
Monocyte-Macrophage Precursor Cells" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
This graph shows the total number of publications written about "Monocyte-Macrophage Precursor Cells" by people in this website by year, and whether "Monocyte-Macrophage Precursor Cells" was a major or minor topic of these publications. (ouhsc.edu)
Below are the most recent publications written about "Monocyte-Macrophage Precursor Cells" by people in Profiles. (ouhsc.edu)

Analyzing 818 ligand-receptor interaction pairs within and between cell lineages, we identify broadly interacting cells, including AT2, innate lymphocytes (ILCs), and basophils. (nih.gov)
[ 1 , 2 ] Macrophage activation syndrome is characterized by pancytopenia, liver insufficiency, coagulopathy, and neurologic symptoms and is thought to be caused by the activation and uncontrolled proliferation of T lymphocytes and well-differentiated macrophages, leading to widespread hemophagocytosis and cytokine overproduction. (medscape.com)
The cytotoxic activity of natural killer (NK) and CD8 + T lymphocytes is mediated by the release of cytolytic granules, which contain perforin, granzymes, and other serinelike proteases, to the target cells. (medscape.com)
Several independent genetic loci related to the release of cytolytic granules have been associated with FHLH, and mutations at this level cause a severe impairment of cytotoxic function of NK cells and cytotoxic T lymphocytes (CTLs) in patients with FHLH. (medscape.com)
LYMPHOCYTES (B and T cells) derive continuously from the same multipotential stem cells that produce myeloid cells, including erythrocytes, granulocytes and macrophages. (pasteur.fr)
Although such swabs are likely to be contaminated with lymphocytes from the tonsils or pharynx, the authors noted that the virus was also found in cell-free supernatants. (cdc.gov)
Histologically the lungs of WPS-exposed mice had foci of mixed inflammatory cells infiltration in the interalveolar interstitium which consisted of neutrophils, lymphocytes and macrophages. (who.int)

The findings are published in mBio in an article titled, " A new tractable method for generating human alveolar macrophage-like cells in vitro to study lung inflammatory processes and diseases . (genengnews.com)
Macrophages (Mφs) are the critical regulators involved in initiation, propagation, and resolution of inflammatory response throughout the tissue regenerative process. (frontiersin.org)
Our data indicate that the Sia + fraction of IVIG possesses the ability to influence inflammatory processes in multiple immune cell types and induces novel signatures in cell surface marker expression and cytokine/chemokine production. (scirp.org)
Our findings suggest that umbilical cord-derived MSCs' conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote anti-inflammatory macrophage polarization. (biomedcentral.com)
Umbilical cord-derived MSCs' conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages. (biomedcentral.com)
Non-activated M0 macrophages can be activated toward pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes, thus playing important and diverse roles at all stages of inflammatory response. (biomedcentral.com)
MSCs have been shown to suppress both the transition of human macrophages from the M0 to M1 state and the pro-inflammatory activity of macrophages already polarized into the M1 state [ 4 ]. (biomedcentral.com)
Myeloid cell populations isolated from colonic inflammatory bowel disease (IBD) or murine dextran sulphate sodium (DSS) induced colitis were assessed using flow cytometry and compared to healthy controls. (frontiersin.org)
However, how inflammatory and anti-inflammatory environments affect macrophage mitochondrial function is poorly understood. (nature.com)
This study aims to explore the energy metabolism of macrophages under either inflammatory or anti-inflammatory conditions by adding LPS and Met, respectively, using an in vitro and an in vivo model. (nature.com)
Pulmonary cytotoxicity, inflammatory cell influx, and relative mRNA expression of genes related to macrophage function and recruitment (Ccl2, Ccl22, and Arg1) were increased as a result of MWCNT exposure but not affected by OPN deficiency. (cdc.gov)

The same applies to the bone marrow that requires macrophages for the clearance of the nuclei that get expelled from erythroblasts during their maturation towards erythrocytes [ 8 ]. (hindawi.com)
In the 1980's, Arnold Caplan and his colleagues published an isolation method of fibroblast-like stromal cells from bone marrow and first identified them as mesenchymal stem cells (MSCs) because of their multilineage differentiation potential ( Caplan, 1991 ). (frontiersin.org)
Histopathologic criterion: Evidence of macrophage hemophagocytosis is found in the bone marrow aspirate sample. (medscape.com)
Here, we investigate the function of MM-derived MIF in the MM microenvironment by examining its effects on bone marrow stromal cells (BMSC). (biomedcentral.com)

The majority of studies on cancer stem cells have been carried out in vitro or in immunodeficient mice models, which do not account for a fully functioning immune response. (newkerala.com)
These effects are replicated in vitro by incubating wild-type human or rodent macrophages, but not macrophages from CB 1 R-deficient ( Cnr1 −/− ) or Nlrp3 −/− mice, with the endocannabinoid anandamide. (nature.com)
We show that macrophages can eliminate melanoma cells in vitro from rapidly proliferating clusters formed on non-adhesive materials. (aiche.org)
Thus, our in vitro and in vivo models utilizing gene-edited cells, monoclonal antibodies for opsonization and checkpoint blockade, and cell therapy begin to define the parameters required for successful immunotherapy of solid tumors including (i) macrophage density, (ii) inhibitory checkpoint disruption, and (iii) IgG-opsonization. (aiche.org)
We also performed cell proliferation, cell migration and tubule formation in vitro experiments to assess the effects of SBP on endothelial cell function and signaling pathways by stimulating macrophage activity. (visualsonics.com)
Results: The in vitro experiment results showed that SBP could significantly increase the expression of mRNAs and proteins associated with angiogenesis in endothelial cells by activating macrophages to release pro-angiogenic factors such as Vegf-a. (visualsonics.com)
Dendritic cells and macrophages have been attributed with stimulatory capacity for in vivo and in vitro immune responses. (diabetesjournals.org)
We investigated this hypothesis in vitro in stimulated murine macrophage RAW cells, which express the inducible form of NOS (iNOS). (rutgers.edu)

Most tissues harbor resident mononuclear phagocytes, that is, dendritic cells and macrophages. (hindawi.com)
Therefore, the differential ability of dendritic cells and macrophages (splenic adherent cells [SACs]) to stimulate pancreatic islet allograft rejection in reversed alloxan-induced diabetic rats was examined. (diabetesjournals.org)

while the proliferation of T cells was unchanged, their antitumor activity was suppressed due to the immunosuppressive factors produced by senescent macrophages. (newkerala.com)
The team also demonstrated that supplementing the mice inoculated with cancer stem cells with a molecule called nicotinamide mononucleotide resulted in the proliferation of non-senescent macrophages and reduced the immunosuppressive factors produced by senescent macrophages, preventing tumour growth and leading to increased survival times in mice. (newkerala.com)
Macrophages and giant cells composed of macrophages are abundant in many neoplasms, but it is unknown whether they can phagocytose target cancer cells that adhere to one another or whether phagocytosis can outcompete proliferation. (aiche.org)
Furthermore, OA inhibited the proliferation of U373 human glioblastoma cells, and the activation of signal transducer and activator of transcription-3 (STAT3) in both human macrophages and glioblastoma cells. (spandidos-publications.com)
These results indicate that OA suppresses the M2 polarization of macrophages and tumor cell proliferation by inhibiting STAT3 activation. (spandidos-publications.com)
Activation of macrophages by SBP eventually led to endothelial cell proliferation, migration and tubule formation and increased the expression of p-Akt and p-Erk1/2 proteins in the downstream PI3K/Akt and MAPK/Erk1/2 signaling pathways related to angiogenesis, respectively. (visualsonics.com)
Effects of 72 h treatment with Vitamin D-binding Protein-derived Macrophage-activating Factor (DBP-MAF) on MCF-7 cell proliferation. (iiarjournals.org)
A: Assessment of cell proliferation by methyl-thiazol-tetrazolium (MTT)-based assay. (iiarjournals.org)
B: Assessment of cell proliferation by cell counting. (iiarjournals.org)
In all three media conditions, no differences in RAW cell proliferation rates were observed over 48 hours. (rutgers.edu)

It is critical to study tissue-specific cells to better understand mechanisms of health and disease, and to screen potential new therapies," said Texas Biomed professor Larry Schlesinger, MD, and senior author of the paper. (genengnews.com)
Together, our study demonstrates how whole-tissue signaling interaction map on the single-cell level can broaden our understanding of cellular networks in health and disease. (nih.gov)
Expression of NLRP3 inflammasome and T cell population markers in adipose tissue are associated with insulin resistance and impaired glucose metabolism in humans. (nature.com)
During the last decades, research has focused on the cell-type-specific properties of these cells in culture, which then led to an immunocentric view of their role in disease like if they were primed like T cells to infiltrate target organs to cause tissue damage and drive progressive scaring [ 2 , 3 ]. (hindawi.com)
In this paper we apply the tissue-centric perspective to discuss the role of resident and infiltrating macrophages and dendritic cells in different organs. (hindawi.com)
Furthermore, we discuss how published data supports the view that changing tissue environments induce the well-known different phenotypes of mononuclear phagocytes, a process that not only enforces each of the different environments but also explains the contribution of these cells to the different tissue pathologies. (hindawi.com)
This slightly different perspective may somewhat shape our understanding of macrophage heterogeneity and tissue pathology but certainly also raise new questions for future research. (hindawi.com)
Due to their considerable plasticity and heterogeneity, the tissue-based DC and macrophage populations have been defined as mononuclear phagocytes [ 1 , 6 , 7 ]. (hindawi.com)
Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
Macrophages are part of the immune system and are associated with tissue repair. (britishlivertrust.org.uk)
That more dendritic cells were required to stimulate rejection in reversed diabetic recipients compared with nondiabetic recipients suggests that other factors, such as the diabetic state and the production of a tolerant status achieved by larger amounts of grafted tissue, may influence graft survival. (diabetesjournals.org)
The findings link tissue inflammation, stromal cell-mediated collagen crosslinking and stiffening to tumour aggression and identify lysyl hydroxylase 2 as a stromal biomarker. (escholarship.org)
Activation of macrophages into different states is a finely tuned process essential for proper resolution of inflammation and tissue repair. (biomedcentral.com)
While macrophage-induced inflammation is crucial for the response to pathogen infection, excessive or sustained inflammation can cause tissue damage and may lead to different conditions such as diabetes, cardiovascular diseases, and tumors 2 . (nature.com)
Data from nonhuman primate models and human autopsy cases suggest that EVD severity is not a direct effect of tissue damage resulting from destruction of infected cells because only foci of necrosis are observed ( 1 , 2 ). (cdc.gov)
Glycoprotein non-metastatic protein B (GPNMB), a remarkable molecule preferentially expressed by tumor-associated macrophages (TAMs), was significantly highly expressed in the OSCC tissue . (bvsalud.org)

The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which causes hematopoietic stem cells to differentiate into macrophages or other related cell types. (wikipedia.org)
This type of hyperinflammatory state is reminiscent of 2 rheumatologic disorders known as macrophage activation syndrome and hemophagocytic lymphohistiocytosis, which are characterized by macrophage and T-cell activation. (cdc.gov)

Serum components, like serum amyloid P component (SAP), together with membrane receptors on phagocytes play essential roles in the phagocytosis of apoptotic cells. (biomedcentral.com)
We evaluated SAP binding to early and late apoptotic cells and whether this binding has functional consequences for the phagocytosis of these cells. (biomedcentral.com)
Phagocytosis was performed by incubation of macrophages for 30 minutes with EA or LA cells in the presence of human serum (HS) and depicted as phagocytosis index (PI, number of Jurkatt internalized by 100 macrophages). (biomedcentral.com)
This might have consequences for diseases in which phagocytosis of early apoptotic cells is decreased. (biomedcentral.com)
OBJECTIVE: To study the phagocytosis of macrophages on the early biofilm cells of E. coli on polydimethylsiloxane, a biomaterial with different surface hardness. (syr.edu)
CFU cell counting, flow cytometry and inverted fluorescence microscope were used to detect the difference in macrophages Phagocytosis of E. coli biofilm cells on surface hardness materials. (syr.edu)
CFU cell count was applied to detect macrophages phagocytosis of E. coli biofilm cells on materials with different surface hardness. (syr.edu)
RESULTS AND CONCLUSION: (1) Bacteria were inoculated first and then macrophages were added to the experiment: CFU cell count, flow cytometry technology and inverted fluorescence microscope detection showed that as the surface hardness of the material increased, the phagocytosis of macrophages increased and the number of E. coli biofilm cells decreased. (syr.edu)
2) Macrophages were inoculated first and then bacteria were added to the experiment: CFU cell count test showed that as the surface hardness of the material increased, the phagocytosis of macrophages increased and the number of bacterial cells decreased. (syr.edu)
3) The results show that with the increase of the hardness of polydimethylsiloxane, the efficiency of macrophages phagocytosis of E. coli biofilm cells is higher. (syr.edu)
The process of a cell ingesting a microorganism, another cell, or cell fragments is called phagocytosis, and cells that ingest are called phagocytes. (msdmanuals.com)

It was demonstrated that cancer stem cells cause macrophages, which are immune cells in charge of the initial stage of cancer cell death, to undergo senescence. (newkerala.com)
Additionally, characterization of the tumor microenvironment such as interactions with immune cells remain largely unknown. (springer.com)
MSCs are reported to contribute both to the inflammation initiation and to its resolution via complex cross talk with immune cells, most importantly macrophages [ 1 ]. (biomedcentral.com)
The gastrointestinal (GI) tract is exposed to the greatest antigenic burden in the body, which poses a significant challenge to the many resident immune cells ( 1 ). (frontiersin.org)
Neutrophils, the most common type of white blood cell in the bloodstream, are among the first immune cells to defend against infection. (msdmanuals.com)

Shigella flexneri, the etiologic agent of bacillary dysentery, invades epithelial cells as well as macrophages and dendritic cells and escapes into the cytosol soon after invasion. (strath.ac.uk)
We have determined the complete gene expression profiles for S. flexneri infecting human epithelial HeLa cells and human macrophage-like U937 cells. (strath.ac.uk)
Epithelial-targeted expression of the crosslinking enzyme, lysyl oxidase, had no impact on collagen crosslinking in PyMT mammary tumours, whereas stromal cell targeting did. (escholarship.org)
ZA therapy increased neoplastic epithelial and endothelial cell apoptosis without affecting hyperproliferation, indicating that ZA was not antimitotic. (jci.org)
Epithelial cells. (cdc.gov)
Furthermore, paracrine GPNMB played a critical role in triggering tumor -promoting activities such as facilitating tumor cell migration , invasion, and epithelial-mesenchymal transition (EMT). (bvsalud.org)

The aim of the present study was to determine the pro-angiogenic effects and mechanism of SBP during inflammation or ischemic pathological conditions and elucidate its regulatory effects on endothelial cell function and signaling pathways mediated by macrophages. (visualsonics.com)
The egress of Candida hyphae from macrophages facilitates immune evasion, but it also alerts macrophages to infection and triggers inflammation. (monash.edu)
Macrophages are pivotal in coordinating a range of important processes in the intestines, including controlling intracellular infections and limiting damaging inflammation against the microbiota. (frontiersin.org)
Previous studies have shown the accumulation of human CD14 Hi or CD14 + HLA-DR Int cells in the GI tract lamina propria (LP) during inflammation ( 2 , 6 , 11 - 14 ). (frontiersin.org)
Therefore, studying the correlation between inflammation and macrophage mitochondrial function could lead to a better understanding of the link between inflammation and diseases. (nature.com)

We noted direct coculturing increased stemness among CSC populations and induced both M1 (CD80 and HLA-DR) and M2 (CD163) tumor associated macrophage polarization. (springer.com)
These studies suggest that there is a bi-directional crosstalk between macrophages and CSCs that promotes stemness renewal and tumor associated macrophage polarization. (springer.com)
Sia + IgG also increased the expression of cell surface markers associated with macrophage polarization (e.g. (scirp.org)
Ovarian cancer stem cells induce the M2 polarization of macrophages through the PPARγ and NF-κB pathways. (stembook.org)
Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs' conditioned media and their effect on macrophages polarization was assessed. (biomedcentral.com)

I am excited to see the full potential of the alveolar macrophage-like cells and if they can be integrated into next-generation lung organoids," Schlesinger added. (genengnews.com)
Antibody-depletion strategies, diphtheria toxin-mediated selective depletion of basophils, and co-culture studies show that lung resident basophils are important regulators of alveolar macrophage development and function. (nih.gov)
This cell division was taking place within an alveolar macrophage. (cdc.gov)

We have previously shown that the sialic acid-containing (Sia + ) fraction of intravenous immune globulin (IVIG) influences cell surface marker expression and cytokine/ chemokine secretion during the differentiation and maturation of human dendritic cells (DC). (scirp.org)
There is a growing body of evidence that multipotent mesenchymal stromal cells' (MSCs') remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. (biomedcentral.com)

Solid tumors pose significant challenges for immunotherapies including those employing phagocytic macrophages as effector cells. (aiche.org)
Micropipette aspiration rheology of cell clusters and freshly isolated mouse tumors revealed viscoelasticity and cell cohesion in both tissues. (aiche.org)
Incubation of tumor cells with T cell-derived IFNγ and TNFα recapitulated the chemokine profile observed in vivo , confirming the capacity of antitumor CD8 T cells to mediate macrophage infiltration of tumors. (aacrjournals.org)
Strikingly, complete regressions of large established tumors depended on the tumor-infiltrating macrophages that were induced by this immunotherapy, because a small-molecule drug inhibitor targeting CSF-1R diminished the number of intratumoral macrophages and abrogated the complete remissions. (aacrjournals.org)
Zhu L, Narloch JL, Onkar S , et al Metastatic breast cancers have reduced immune cell recruitment but harbor increased macrophages relative to their matched primary tumors. (bmj.com)
We here investigated the prognostic impact of tumor-infiltrating CD169 + macrophages in primary tumors (PTs) and their spatial relation to tumor-infiltrating B and T cells. (lu.se)

1 × 10 6 MM.1S-luc cells (ShE control n = 10, and ShMIF n = 7) were injected via the tail vein of 6-8-week-old NSG mice. (biomedcentral.com)

Indeed, increases in [Ca2+]i are essential for phagosome-granule (lysosome) fusion in neutrophils and for lysosomal fusion events that mediate host cell invasion by Trypanosoma cruzi trypomastigotes. (rupress.org)
Neutrophils contain granules that release enzymes to help kill and digest these cells. (msdmanuals.com)

The assay reveals that Candida escapes by using two pore-forming proteins to permeabilize macrophage membranes: the fungal toxin candidalysin and Nlrp3 inflammasome-activated Gasdermin D. Candidalysin plays a major role in escape, with Nlrp3 and Gasdermin D-dependent and -independent contributions. (monash.edu)
Autophagy is one of the major digestive processes in cells and plays a critical role in maintaining cell homeostasis through delivering cytoplasmic materials, such as lipids, proteins, and organelles to lysosomes, for degradation 13 . (nature.com)
Cells' status can be inferred by examining autophagy proteins such as ATG8, as well as measuring ROS and mitochondrial membrane potential. (nature.com)
Macrophages secrete a number of growth factors including a class of proteins called Wnt. (hairlosscure2020.com)

Tumor-promoting functions of macrophages include angiogenesis, metastasis formation, and suppression of Th1-type immune responses. (aacrjournals.org)
Conclusion: Shexiang Baoxin Pills can promote angiogenesis by activating macrophages to regulate endothelial cell function and signal transduction pathways. (visualsonics.com)

The team identified interleukin 6 (IL-6) produced by cancer stem cells as the molecule responsible for triggering these effects. (newkerala.com)
To investigate tumor necrosis factor α (TNF α) and interleukin-1β (IL-1 β) regulation of CCL3 expression in nucleus pulposus (NP) cells and in macrophage migration. (shu.ac.uk)

Peripheral CB 1 R blockade, in vivo depletion of macrophages or macrophage-specific knockdown of CB 1 R reverses or prevents these changes and restores normoglycemia and glucose-induced insulin secretion. (nature.com)
This theme seems to be common in bacterial infection, because the Ipa-Mxi-Spa-like type III secretion systems were also down-regulated during mammalian cell infection by Salmonella enterica serovar Typhimurium and Escherichia coli O157. (strath.ac.uk)
In these studies, the research team successfully used Imaris to visualize the long-standing interactions between CD169+ macrophages and iNKT cells and found very fast iNKT activation and cytokine secretion. (labbulletin.com)
To investigate the effects of MIF secretion by MM on its microenvironment, we used cytokine arrays to establish if cytokine changes occur when MM cells are cultured with primary BMSC. (biomedcentral.com)

Tetrahydrobiopterin is required for cytokine-induced nitric oxide production in a murine macrophage cell line (RAW 264). (aspetjournals.org)
The results presented here support previous suggestions, based on experiments with isolated enzymes, that BH4 is absolutely required for cytokine-stimulated nitric oxide production in macrophages and they suggest that only a small fraction of the total intracellular BH4 pool in macrophages is utilized in the production of fully active nitric oxide synthase. (aspetjournals.org)
M-CSF is a cytokine, being a smaller protein involved in cell signaling. (wikipedia.org)
Pretreatment of macrophages with an antagonist of CCR1, the primary receptor for CCL3 and CCL4, blocked cytokine-mediated migration. (shu.ac.uk)
c Representative ( n = 3) Human XL cytokine array output after a 24-h incubation in either mono or co-culture, cell supernatant was used for analysis. (biomedcentral.com)

ice with healthy immune systems are exposed to cancer stem cells, which cause macrophage ageing and tumour growth. (newkerala.com)
A variety of cells, the most significant of which are cancer stem cells, make up malignant tumours. (newkerala.com)
A group of researchers at Hokkaido University's Institute for Genetic Medicine, under the direction of Associate Professor Haruka Wada, looked into the ways in which cancer stem cells avoid the immune system in mouse models. (newkerala.com)
further investigation showed that macrophages cultured with cancer stem cells exhibit senescence or cellular ageing. (newkerala.com)
Cancer stem cells (CSCs) are thought to the underlying reason for cancer initiation, metastasis, and relapse. (springer.com)
Cancer stem cells (CSCs) represent a small subset of the tumor population and reside at the apex of the hierarchy [ 9 ]. (springer.com)
Almost all patients with GBM relapse within a narrow margin around the initial resected lesion due to postsurgery residual glioma stem cells (GSCs). (nih.gov)
Tri- and bipotential myeloid intermediates between the multipotential stem cells and later unipotential cells have been identified using clonal methods in culture. (pasteur.fr)
In 2008, Spanish doctors performed the world's first stem cell engineered windpipe transplant in a woman using a donor trachea and the woman's own stem cells. (hairlosscure2020.com)
However, two days ago, Spanish scientists (from the previously mentioned cancer research institute CNIO) led by Dr. Mirna Perez-Moreno and Dr. Donatello Castellana published a groundbreaking hair loss research related paper in the PLOS Biology journal titled "Macrophages contribute to the cyclic activation of adult hair follicle stem cells. (hairlosscure2020.com)
Macrophages (a type of white blood cell) can activate adult hair follicle stem cells. (hairlosscure2020.com)

Macrophage colony-stimulating factor has been shown to interact with PIK3R2. (wikipedia.org)

Aggregation of phagocytic macrophages within clusters and the nonlinear dependence of the cluster growth rate on macrophage number suggest that macrophages may exhibit cooperativity in engulfing solid tumor targets, which resembles the foreign body response to biomaterials in some ways. (aiche.org)

Endocannabinoids contribute to insulin resistance through activation of peripheral CB 1 receptors (CB 1 Rs) and also promote beta cell failure. (nature.com)
Here we show that beta cell failure in adult ZDF rats is not associated with CB 1 R signaling in beta cells, but rather in M1 macrophages infiltrating into pancreatic islets, and that this leads to activation of the Nlrp3-ASC inflammasome in the macrophages. (nature.com)
These findings implicate endocannabinoids and inflammasome activation in beta cell failure and identify macrophage-expressed CB 1 R as a therapeutic target in T2DM. (nature.com)
Without glycoconjugate (GC) activation, murine macrophages were killed by Bacillus toxins. (listlabs.com)
Increased levels of inducible nitric oxide (NO) production by macrophages in presence of GCs suggest that GCs provide an activation signal for macrophages and stimulate their function. (listlabs.com)
Results hint to GCs that promote neutralization of Bacillus toxins, block toxin-induced macrophage death, while increasing macrophage activation. (listlabs.com)
Our findings indicate that TNF α and IL-1 β modulate the expression of CCL3 in NP cells by controlling the activation of MAPK, NF-κB, and C/EBP β signaling. (shu.ac.uk)
Macrophage activation syndrome (MAS) is a life-threatening complication of rheumatic disease that, for unknown reasons, occurs much more frequently in individuals with systemic juvenile idiopathic arthritis (SJIA) and in those with adult-onset Still disease. (medscape.com)
Typically, patients with macrophage activation syndrome become acutely ill with the sudden onset of nonremitting high fever, profound depression in all 3 blood cell lines (ie, leukopenia, anemia, and thrombocytopenia), hepatosplenomegaly, lymphadenopathy, and elevated serum liver enzyme levels. (medscape.com)
The treatment of macrophage activation syndrome is traditionally based on the parenteral administration of high doses of corticosteroids. (medscape.com)
Macrophage activation syndrome is characterized by a highly stimulated but ineffective immune response. (medscape.com)
Recent studies have shown that MUNC 13-4 polymorphisms are associated with macrophage activation syndrome in some patients with SJIA. (medscape.com)
An evaluation of 2 cohorts of patients with EVD revealed that a marker of macrophage activation (sCD163) but not T-cell activation (sCD25) was associated with severe and fatal EVD. (cdc.gov)
These data suggest that host macrophage activation contributes to EVD pathogenesis and that directed antiinflammatory therapies could be beneficial in the treatment of EVD. (cdc.gov)
Hypercytokinemia accompanied by severe clinical disease seen in EVD is reminiscent of what has been described for macrophage activation syndrome (MAS) and hemophagocytic lymphohistiocytosis (HLH). (cdc.gov)

Although similar methods have detected committed pre-B cells in mouse fetal liver, earlier progenitors with additional non-B lineage options have not been demonstrated in normal tissues. (pasteur.fr)
The availability of such cells in purified form will allow direct study of lineage choice in cells having both lymphoid and non-lymphoid options. (pasteur.fr)
Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
Introduction regulate choices of expressed genes as part of the macrophage/ neutrophil lineage. (lu.se)

Mononuclear phagocytes are a group of phenotypic distinct members, often referred to as either macrophages or dendritic cells (DC), that derive from myeloid precursors and that contribute to the functions of peripheral tissues [ 1 ]. (hindawi.com)
Macrophages form the largest component of the intestinal mononuclear phagocyte system and play a pivotal role in mediating immune homeostasis ( 2 ). (frontiersin.org)

The team used two cell lines of glioblastoma tumour, one of which was capable of inducing tumour formation (cancer stem cell) and the other of which was not. (newkerala.com)
In an orthotopic patient-derived glioblastoma humanized mouse model, the combined treatment with nanoporter-hydrogel superstructure and CD47 antibody increased the frequency of positive immune responding cells and suppressed the negative immune regulating cells, conferring a robust tumoricidal immunity surrounding the postsurgical cavity and inhibiting postoperative glioblastoma relapse. (nih.gov)

Here, we report a cavity-injectable nanoporter-hydrogel superstructure that creates GSC-specific chimeric antigen receptor (CAR) macrophages/microglia (MΦs) surrounding the cavity to prevent GBM relapse. (nih.gov)
The analyses implicated cellular and molecular targets of ZA's actions: ZA suppressed MMP-9 expression by infiltrating macrophages and inhibited metalloprotease activity, reducing association of VEGF with its receptor on angiogenic endothelial cells. (jci.org)
El ligando es específico para el RECEPTOR 4-1BB y puede desempeñar un papel en la indución de la proliferación de los LINFOCITOS T activados de la sangre periférica. (bvsalud.org)

Comparison with the gene expression of intracellular Salmonella serovar Typhimurium, which resides within the Salmonella-containing vacuole, indicated that S. flexneri is exposed to oxidative stress in U937 cells. (strath.ac.uk)
Intracellular recordings of cultured mouse thioglycolate-induced peritoneal exudate macrophages reveal that these cells can exhibit two different types of electrophysiological properties characterized by differences in their current-voltage relationships and their resting membrane potentials. (rupress.org)
Since several intracellular pathogens survive in macrophage phagosomes that do not fuse with lysosomes, we examined the regulation of phagosome-lysosome fusion in macrophages. (rupress.org)

These tissues harbour the highest number of tumour-associated macrophages, whose therapeutic ablation in experimental models reduced metastasis, and decreased collagen crosslinks and stromal stiffening. (escholarship.org)

Abundant macrophage infiltration of solid cancers commonly correlates with poor prognosis. (aacrjournals.org)
The CCL3-CCR1 axis may play an important role in promoting macrophage infiltration in degenerated, herniated discs. (shu.ac.uk)

The common origin of the two cell types was shown by culture of single precursor cells. (pasteur.fr)

Challenge stimuli consisting of ≤ 10 5 SACs or ≤10 4 dendritic cells failed to induce graft rejection. (diabetesjournals.org)
Jurkat cells were irradiated with UVB to induce apoptosis. (biomedcentral.com)

An immune-centric view assumes that a particular priming of phagocytes then causes a particular type of pathology in target tissues, conceptually similar to antigen-specific T-cell priming. (hindawi.com)
All solid organs and most other tissues harbor a network of DC or macrophages (Table 1 ). (hindawi.com)
Acquired Immunity One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)
Macrophages stay in the tissues. (msdmanuals.com)

However, both IgG opsonization and disruption of the macrophage checkpoint ligand CD47 by CRISPR knockout or antibody blockade were required to achieve elimination. (aiche.org)
HIV preferentially infects and replicates in cells expressing the CD4 molecule, the ligand for viral attachment and subsequent cell entry (1). (cdc.gov)

After LPS exposure, nitric oxide production in the LowB2 and LowFA cells was 30-35% and 35-40% of the control cells, respectively (p ≤ 0.001). (rutgers.edu)
LPS-induced nitric oxide production is reduced in RAW cells grown in either riboflavin or folic acid deficient media independent of iNOS expression. (rutgers.edu)

A classification that sufficiently covers their phenotypic heterogeneity and plasticity during homeostasis and disease does not yet exist because cell culture-based phenotypes often do not match those found in vivo . (hindawi.com)
The Lymphocyte Interaction Laboratory was the first team to establish a role for a group of macrophages in acquiring antigen and presenting intact antigen to follicular B cells in lymph nodes in vivo . (labbulletin.com)

Oral squamous cell carcinoma (OSCC) is the most prevalent form of oral and maxillofacial malignancies , characterized by a low five-year survival rate primarily caused by invasion and metastasis . (bvsalud.org)
The progression of OSCC is influenced by macrophage -mediated immunosuppression , which contributes to both local invasion and distant metastasis . (bvsalud.org)

These changes of the intratumoral myeloid composition coincided with macrophage recruitment by chemokines, including CCL2 and CCL5, and were completely dependent on a vaccine-induced influx of tumor-specific CD8 T cells. (aacrjournals.org)

Alveolar macrophages (AMs) are unique lung resident cells that contact airborne pathogens and environmental particulates," wrote the researchers. (genengnews.com)
The generated cells are 94% genetically similar to human alveolar macrophages collected from lung washes. (genengnews.com)
Lung development and function arises from the interactions between diverse cell types and lineages. (nih.gov)
Using single-cell RNA sequencing (RNA-seq), we characterize the cellular composition of the lung during development and identify vast dynamics in cell composition and their molecular characteristics. (nih.gov)

A vaccine containing partial cellular material as opposed to complete cells. (cdc.gov)

GCs were shown to have a protective influence, sparing macrophages from toxin-induced cell death, as shown by increased macrophage cell viability based on trypan blue assay. (listlabs.com)
Although this binary classification does not take into account the variety of subcategories of macrophages phenotypes [ 2 ], it can be useful for studying the mechanisms of macrophage viability and activity regulation [ 3 ]. (biomedcentral.com)

Given its track record in clinical use with limited toxicity, ZA holds promise as an "unconventional" MMP-9 inhibitor for antiangiogenic therapy of cervical cancer and potentially for additional cancers and other diseases where MMP-9 expression by infiltrating macrophages is evident. (jci.org)

Macrophages secrete substances that attract other white blood cells to the site of the infection. (msdmanuals.com)

Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma. (bvsalud.org)

Based on the findings above we further hypothesized that the decreased NO output from the B vitamin-deficient cells was due to insufficient BH4 availability to the cells. (rutgers.edu)
Our findings explain how Candida escapes from macrophages by using three strategies: permeabilizing macrophage membranes via candidalysin and engaging two host cell death pathways, Gasdermin D-mediated pyroptosis and ETosis. (monash.edu)
Collectively, our findings decipher a positive feedback loop in which tumor cells metabolically interact with macrophages in the OSCC microenvironment, highlighting the potential for therapeutic targeting of the GPNMB/CD44 axis as a promising strategy for treating OSCC. (bvsalud.org)

Stromal cells in microdissected human tumours expressed the highest level of collagen crosslinking enzymes. (escholarship.org)
Multipotent mesenchymal stromal cells (MSCs) are an extensively researched yet still understudied tool for treating a wide range of pathological conditions, one particular area of research interest being their immunomodulatory properties. (biomedcentral.com)

Herein, it is of great necessity to explore the molecular mechanisms underlying the crosstalk between OSCC cells and macrophages , as it remains unclear. (bvsalud.org)

Anatomy38

Organisms26

Diseases10

Chemicals and Drugs112

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes50

Disciplines and Occupations1

Technology, Industry, Agriculture2

Information Science3

Phenotype11

Cytokines9

Monocytes18

Monocyte9

Lymphocytes7

Inflammatory11

Bone marrow4

Vitro8

Dendritic cells and macrophages2

Proliferation10

Tissue16

Known as macrophage2

Phagocytosis11

Immune cells5

Epithelial6

Inflammation5

Polarization5

Alveolar macrophage3

Differentiation2

Tumors6

Mice1

Neutrophils2

Proteins4

Angiogenesis2

Interleukin2

Secretion4

Cytokine5

Stem Cells11

Colony-stimul1

Phagocytic1

Activation15

Lineage4

Mononuclear2

Glioblastoma2

Receptor3

Intracellular3

Tumour1

Infiltration2

Precursor cells1

Induce2

Tissues4

Ligand2

Nitric oxide production2

Vivo2

Metastasis2

Chemokines1

Lung4

Cellular1

Viability2

Cancers1

Secrete1

Tumor cell1

Findings3

Stromal cells2

Crosstalk1