Cells by lysis growth cell cycle. Medical search. Frequent questions

SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
Also the e effect of the cross-linking monomer on bacterial growth inhibition, lysis efficiency and the mechanical stability of the PPM column within the microfluidic channel, using three different cross-linking monomers was studied. (uwaterloo.ca)
The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. (medsci.org)
In mice injected with human breast and ovarian cancer cells, fasting cycles promoted survival extension by protecting the mice from chemotherapy while causing a strong inhibition of tumor progression. (kingdomfasting.com)
Mechanistically, the cell differentiation mediated by JOA may be originated from the inhibition of BCR-ABL/c-MYC signaling in CML cells expressing wild-type BCR-ABL and BCR-ABL-T315I. (jcancer.org)
Since both natural supplements and direct regulation of PrP c expression contribute to inhibition of cancer progression and growth, we hypothesized that knockdown of PrP c could lead to an enhanced synergic effect on the inhibition of cancer growth by fucoidan. (iiarjournals.org)
Conclusion: Combination of fucoidan with silencing of PrP c has a synergic effect on the inhibition of HT29 colon cancer cell growth. (iiarjournals.org)
The inhibition of NAMPT is usually shown to induce cell death and reduce osteoclastogenesis in MM [10,11]. (forgetmenotinitiative.org)
Due to the selective expression of UCK2 in malignancy cells, a selective inhibition of this important enzyme necessitates the discovery of its potential inhibitors for malignancy chemotherapy. (nanoker-society.org)
SFK inhibition also induced muscle differentiation in RMS cells by affecting the NOTCH3 receptor-p38 mitogen-activated protein kinase (MAPK) axis, which regulates the balance between proliferation and differentiation. (oncotarget.com)
Overall, our findings suggest that SFK inhibition, besides reducing RMS cell growth and invasive potential, could also represent a differentiation therapeutic strategy for RMS. (oncotarget.com)
In some cases, such as the c-Kit mutation observed in GIST, tumour cells can be highly "addicted" to one or more oncogenic kinases 2-4 and the inhibition of these kinases with a TKI can lead to a striking tumour response and trigger tumour lysis syndrome (TLS). (touchoncology.com)

The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. (medsci.org)
In 4T1 breast cancer cells, short-term starvation resulted in increased phosphorylation of the stress-sensitizing Akt and S6 kinases, increased oxidative stress, caspase-3 cleavage, DNA damage, and apoptosis. (kingdomfasting.com)
Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. (aacrjournals.org)
Chronic myeloid leukemia (CML) results from BCR-ABL oncogene, which blocks CML cells differentiation and protects these cells from apoptosis. (jcancer.org)
Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
Furthermore, silencing PrP c expression with si-PRNP amplified the fucoidan-induced changes in cell proliferation, apoptosis, and migration. (iiarjournals.org)
Cell proliferation was decided using MTT assay, while apoptosis was analyzed with circulation cytometry using Annexin V-fluorescein isothiocyanate/propidium iodide assay. (forgetmenotinitiative.org)
or gene silencing significantly inhibited cell proliferation and induced apoptosis in RPMI 8226 cells ( (forgetmenotinitiative.org)
SI221, which showed to be mainly effective against the SFK member YES, significantly reduced cell viability and induced apoptosis, without affecting non-tumor cells, such as primary human skin fibroblasts and differentiated C2C12 cells. (oncotarget.com)
As opposed to cytotoxic chemotherapy, TKIs target specific mutated kinases that promote tumour angiogenesis and neoplastic cells growth, proliferation, and resistance to apoptosis. (touchoncology.com)

Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
However, most of our knowledge about cell proliferation comes from studies that average data from large and mixed cell populations. (frontiersin.org)
In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. (medsci.org)
We found that JOA at lower concentration significantly inhibited the proliferation of CML cells expressing mutant BCR-ABL (T315I mutation included) and wild-type BCR-ABL, which was due to that JOA induced the cell differentiation and the cell cycle arrest at G0/G1 phase. (jcancer.org)
BCR-ABL oncogene encodes a BCR-ABL tyrosine kinase (TK), which triggers different downstream targets including c-Myc, STAT5 and CrkL, which participated in the control of cell differentiation, proliferation, migration and other cellular behaviors [ 3 - 5 ]. (jcancer.org)

RNAseq and analysis of bulk samples40000 LCs and 5000 BCs were isolated by FACS as described above and collected into kit lysis buffer. (nih.gov)
The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. (creativebiomart.net)
The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. (creativebiomart.net)
Western blot analysis Cellular lysates were prepared by suspending 1 106 cells in a lysis buffer at 4oC for 30 min. (alk-inhibitors.com)
After incubation for 40 min at 37oC, samples were loaded onto 1 agarose gel and subjected to electrophoresis for 1 h at 100 V. Immunofluorescence analysis Cells were fixed in PIPES buffer containing 2 paraformaldehyde and 0.1 glutaraldehyde for 10 min at room temperature. (alk-inhibitors.com)
Extraction of monomeric and polymeric tubulin After treatment with the indicated compounds, cells were washed twice with PBS, then extracted into 0.4 ml of a monomeric extraction buffer, transferred to a new tube, and centrifuged at 13,000 g for 10 min at room temperature. (alk-inhibitors.com)
NP-EGTA AM can serve as a photolabile buffer in cells because, once converted to NP-EGTA by intracellular esterases, it will bind Ca 2+ with high affinity until photolyzed with UV light. (thermofisher.com)

Here, we show that treatment with starvation conditions sensitized yeast cells (Saccharomyces cerevisiae) expressing the oncogene-like RAS2val19 to oxidative stress and 15 of 17 mammalian cancer cell lines to chemotherapeutic agents. (kingdomfasting.com)
By contrast, a limited exposure to a severely restricted diet (short-term starvation or fasting) can protect yeast, mammalian cells, mice, and possibly patients from the toxic effects of oxidative and chemotherapeutic agents without causing chronic weight loss (10-14). (kingdomfasting.com)
Extraction of total protein Total protein was extracted from lysed HT-29 cells in an appropriate volume of ProteoJET mammalian cell lysis reagent (Fermentas, Burlington, ON, Canada). (nanoker-society.org)

Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)
Forced expression of NBPF1 in HEK293T cells resulted in a G1 cell cycle arrest that was accompanied by upregulation of the cyclin-dependent kinase inhibitor p21 CIP1/WAF1 in a p53-dependent manner. (biomedcentral.com)
Additionally, forced expression of NBPF1 in two p53-mutant neuroblastoma cell lines also resulted in a G1 cell cycle arrest and CDKN1A upregulation. (biomedcentral.com)
JIB extract induced cell cycle arrest at the G 0 /G 1 phase and decreased cyclin and cdk protein expressions. (medsci.org)
Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
Furthermore, restoring wild-type HPK1 protein in PDA cells led to the increase in p21 and p27 protein expression and cell cycle arrest. (aacrjournals.org)
The expression of p53 is directly linked to a blockage of cell cycle progression at G0/G1 phase and upregulates Bax, cytochrome studies have shown the ability of the bioactive compounds of flavokawain B and alpinetin to target UCK2 enzyme specifically, inducing cell cycle arrest and subsequently leading to cancer cell death, possibly through interfering the MDM2-p53 signalling pathway. (nanoker-society.org)

We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
The molecular processes behind cell cycle progression have been dissected by numerous morphological studies on live or fixed single cells using a plethora of techniques to visualize components and processes during cell division. (frontiersin.org)
Cytopathic effect (CPE) in these cells was evident earlier for BoHV-5 strain 97/613 and CPE progression was slower for BoHV-1, particularly for Cooper strain. (bvsalud.org)
Here, cycles of fasting for 2 days in the absence of other treatments are shown to delay the progression of several tumor types in mice and, in some cases, to be as effective as toxic chemotherapy drugs. (kingdomfasting.com)
Cycles of starvation were as effective as chemotherapeutic agents in delaying progression of different tumors and increased the effectiveness of these drugs against melanoma, glioma, and breast cancer cells. (kingdomfasting.com)
Also, DR only retards the progression of specific cancers, possibly because of its relatively small effect on glucose and growth factors (7, 8). (kingdomfasting.com)
Background: The putative functions of the cellular prion protein (PrP c ) are believed to be associated with cell signaling, differentiation, survival, and cancer progression. (iiarjournals.org)

Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
Cells deficient in both these proteases are much more amenable to the production of proteins from cloned genes. (neb.com)
Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
The phage remains in a lysogenic state until environmental conditions induce expression of phage lytic cycle genes, leading to new phage production and lysis of the host bacterium. (cdc.gov)
therefore, induction of the lytic cycle increases expression of the toxin genes and host cell lysis allows for toxin release ( 10 ). (cdc.gov)
We investigated by real-time quantitative RT-PCR the expression profile of a panel of genes important in cell cycle regulation. (biomedcentral.com)
Amplified DNA genes were detected by gel electrophoresis starting from small volume and cells concentration down to 10^2 CFU/ml. (uwaterloo.ca)
Targeted therapy drugs are used to target specific genes and proteins of cancer cells to precisely identify and attack specific types of cancer cells. (medsci.org)
Our results offer a even more unique understanding of the jobs of and genes in the molecular pathogenesis of MM. MATERIALS AND METHODS Cell collection The myeloma cell collection RPMI 8226 was purchased from the American Type Culture Collection (ATCC, USA). (forgetmenotinitiative.org)

The multiplexed libraries (18 pM) were loaded on flow cells and sequences were produced using a NovaSeq 6000 S2 Reagent Kit (200 cycles) from a NovaSeq 6000 System (Illumina). (nih.gov)

The effects of NBPF1 on the cell cycle were evaluated by flow cytometry. (biomedcentral.com)
Flow cytometric analysis The cell cycle was analyzed using flow cytometry of PI stained cells. (alk-inhibitors.com)

We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)
The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. (wjgnet.com)
In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. (wjgnet.com)
Thanks to recent progresses, stem cells have been extensively employed to study Plasmodium liver and blood cycle in vitro . (wjgnet.com)
We tested whether natural killer cells expanded by co-culture with K562 cells transfected with 41BBL and membrane-bound interleukin-15 could kill myeloma cells with a high-risk gene expression profile in vitro and in a unique model which recapitulates human myeloma. (haematologica.org)
Moreover, SI221 decreased in vitro cell migration and invasion and reduced tumor growth in a RMS xenograft model. (oncotarget.com)
An image depicting head and neck squamous cell carcinoma in vitro can be seen below. (medscape.com)
Head and neck squamous cell carcinoma in vitro (cell culture). (medscape.com)

Shiga toxins (Stx) are potent AB 5 cytotoxins that inhibit eukaryotic protein synthesis, eventually leading to host cell death ( 1 ). (cdc.gov)
In humans, DR does not reduce growth-promoting insulin-like growth factor 1 (IGF-1) unless it is combined with protein restriction (9). (kingdomfasting.com)
Similarly, HPK1 protein was not expressed in any of eight PDA cell lines examined but was expressed in immortalized human pancreatic duct epithelial (HPDE) cells. (aacrjournals.org)
Treatment of Panc-1 cells with a proteasome inhibitor, MG132, increased the HPK1 protein levels in a dose-dependent manner, suggesting that alteration in proteasome activity contributes to the loss of HPK1 protein expression in pancreatic cancer. (aacrjournals.org)
The NAMPT protein manifestation in siRNA-treated cells was estimated by enzyme-linked immunosorbent assay. (forgetmenotinitiative.org)
Tumour suppressor protein, p53, prevents cancer development by eliminating cells with mutagenic alterations or potential for neoplastic transformation or blocking their cell cycle permanently or by transient DNA repair [3C5]. (nanoker-society.org)
For example, herceptin was the first drug to be used with a matching diagnostic test for treating breast cancer in women whose cancer cells expressed HER2 protein. (biotechfront.com)

Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
JOA displayed the activity of inhibiting the BCR-ABL and promoted differentiation of not only imatinib -sensitive but also imatinib -resistant cells with BCR-ABL mutation, which could become a potent lead compound to overcome the imatinib -resistant induced by inhibitors of BCR-ABL tyrosine kinase in CML therapy. (jcancer.org)

Microorganisms are at the center of all major biogeochemical processes on Earth, providing valuable ecosystem services such as nutrient cycling, climate regulation and degradation of organic matter and pollutants. (jakesonline.org)

In response to DNA damage, a synthetic lethal relationship exists between the cell cycle checkpoint kinase MK2 and the tumor suppressor p53. (nature.com)
Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
Decreased expression of NBPF1 in neuroblastoma cell lines with loss of 1p36 heterozygosity and the marked decrease of anchorage-independent clonal growth of DLD1 colorectal carcinoma cells with induced NBPF1 expression further suggest that NBPF1 functions as tumor suppressor. (biomedcentral.com)
We demonstrated that NBPF1 exerts different tumor suppressive effects, depending on the cell line analyzed, and provide new clues into the molecular mechanism of the enigmatic NBPF proteins. (biomedcentral.com)
Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma. (medsci.org)
Experiments in simple organisms, human cells, and mice indicated that these effects of fasting were caused by changes inside and outside cells that increased the death of tumor but not normal cells, a process termed differential stress sensitization. (kingdomfasting.com)
Although clinical trials testing the effect of fasting in cancer treatment are still in the early stages, they suggest that fasting cycles may boost the efficacy of chemotherapeutic agents and could be as effective as chemotherapy drugs in the killing of specific tumor cells. (kingdomfasting.com)
This highly malignant tumor has traditionally been considered to arise from committed skeletal muscle precursor cells that fail to differentiate [ 1 ], although some RMSs develop in organs lacking a skeletal muscle component [ 2 , 3 ]. (oncotarget.com)

The host cell cycle is also modulated by the toxin, which can act as an intracellular mitogen. (wikipedia.org)

Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)

As an initial step to understand the biology of both BoHV types in neural cells, undifferentiated SH-SY5Y human neuroblastoma cells were infected with BoHV-1 strains Cooper and Los Angeles (LA), BoHV-5 strain 97/613 and A663, a BoHV-5/BoHV-1 natural recombinant. (bvsalud.org)
In mouse models of neuroblastoma, fasting cycles plus chemotherapy drugs-but not either treatment alone-resulted in long-term cancer-free survival. (kingdomfasting.com)

Besides, 12% of patients have second- or third-degree skin reactions that are sensitive to light, and about 18% of patients develop cutaneous squamous-cell carcinoma and keratoacanthoma or occurred at the same time [ 6 ]. (medsci.org)

To determine whether the potentiating effect of extracellular ATP involves cell cycle control mechanisms, we have measured the expression of cyclins that are induced in different phases of the cell cycle in primary cultures of rat cortical astrocytes. (sagepub.com)
Both lysis mechanisms were thus validated. (uwaterloo.ca)
Osteoprogenitor MSCs not only differentiate into bone, but they also exert modulatory effects on immune cells via a variety of mechanisms. (hindawi.com)
[ 6 ] These events lead the cancer cell to escape normal cell growth and control mechanisms, to avoid system control mechanisms (ie, immunologic surveillance), and to establish a nutrient supply. (medscape.com)
Thus, the aim of this work is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, its mechanism of action, and to elucidate molecular mechanisms involved in endotoxin's recognition by the immune system and cell activation. (bvsalud.org)
Therefore, the objective of this study is to carry out a review of the literature on the role of bacterial endotoxin in the etiology of periapical lesions, as well as to elucidate molecular mechanisms involved in its recognition by the immune system and in cell activation. (bvsalud.org)

Cells were cultured in RPMI-1640 medium (ATCC) supplemented with 10% of fetal bovine serum (Lonza, Switzerland). (forgetmenotinitiative.org)

Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit. (antikoerper-online.de)

Deletion of this gene confers resistance to this type of phage, but does not significantly affect the transformation or growth characteristics of the cell. (neb.com)
Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects susceptible bacterial cells, that is composed of DNA and infects most strains of Escherichia coli. (shapeways.com)
Bacteriophage T7 has a lytic life cycle and several properties that make it an ideal phage for experimentation. (shapeways.com)
T7 has a short life cycle of 17 min at 37˚C, i.e. the time from infection to the lysis of the host cell when new phage are released. (shapeways.com)
This adapted phage can undergo an effective expansion of its population by more than 1013 in one hour of growth. (shapeways.com)
Finally, we discuss the benefits of phage therapy beyond the clinical perspective, including opportunities for scientific outreach and effective education, interdisciplinary collaboration, cultural and economic growth, and even innovative use of social media, making the case that phage therapy is more than just an alternative to antibiotics. (asm.org)

All strains released detectable infectious virus in the extracellular medium by 8 hpi, confirming that undifferentiated SH-SY5Y cells are fully permissive to BoHV infection. (bvsalud.org)
Sickle cell disease (SCD) is associated with chronic haemolysis, immuno-suppression and suscepti- bility to infections, which may trigger infection-associated haemolysis (IAH). (bvsalud.org)
0.2 × 10 9 /L), the inflammatory response may be muted, and the usual inflammatory findings of leukocytosis or white blood cells in the urine or at the site of infection may not occur. (msdmanuals.com)

The capsule has been shown in strains of serogroups A and B to help resist phagocytosis by host immune cells and capsule type A has also been shown to help resist complement-mediated lysis. (wikipedia.org)
E. coli B strains are 'naturally' deficient in the Lon protease which in K-12 strains serves to degrade misfolded proteins and to prevent some cell cycle-specific proteins from accumulating. (neb.com)
The OmpT protease resides at the surface of wild type E. coli in both K-12 and B strains, presumably helping the cells to derive amino acids from their external environment. (neb.com)
There were no significant differences in ITS copies estimated by the ddPCR assay between environmental DNA samples from various localities spiked with the same number of cells of culture strains. (e-algae.org)
92% were tropic HIV-1 strains into target cells [ 5-7 ]. (who.int)

Description: English: Visualization of yeast cells (Schizosaccharomyces pombe). (eol.org)
Small molecules are manufactured chemically, but the larger ones are created by human cells, bacterial cells, yeast cells, and animal or plant cells. (biotechfront.com)

Besides CHS, mutation in gene is usually one of the important factors that trigger hemophagocytic lymphohistiocytosis, a insufficiency in resistant program function, and life-threatening disease characterized by uncontrolled macrophage and T-cell account activation [14]. (forgetmenotinitiative.org)
Biotech Europe GmbH, Freiburg, Germa- cation of the virus in cells heterozygous for ny) was used to extract genomic DNA the mutation proceeded at an intermediate from white blood cells following the lysis rate [ 5 ]. (who.int)

Transfection of siRNAs into the RPMI 8226 myeloma cells was performed by Amaxa Nucleofection kit V (Lonza, Switzerland). (forgetmenotinitiative.org)
Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)

Activated SIRT1 signals hypoxia inducible factor (HIF)-2α, which transactivates vascular endothelial growth factor (VEGF) and erythropoietin. (molvis.org)

Our study exhibited that and play pivotal functions in the molecular pathogenesis of MM. This is usually the first statement describing the possible functions of in myelomagenesis and its potential role as a SU 11654 therapeutic target in MM. gene promotes tumorigenesis through constant NAD resynthesis to provide adequate energy for rapidly proliferating malignancy cells [9]. (forgetmenotinitiative.org)

Virulent or obligate lytic phages infect and quickly kill their bacterial host cell, whereas temperate or lysogenic phages may either stably integrate into their host's genome or enter into the lytic life cycle. (asm.org)

Also, the cell lysate containing the DNA released from bacterial cells after lysing with the developed antibacterial PPM columns is ready to be used by PCR with no further purification needed. (uwaterloo.ca)
The cell lysis e efficiency of the biochips were characterized by qualitatively (semi-quantitatively) detecting DNA and quantitatively determining DNA concentration in the crude lysate collected at the outlet of the biochip. (uwaterloo.ca)
By using fluorometry, the ethidium bromide (EtBr) intercalation assay was utilized as an indicator of the presence of DNA in the cell lysate and the DNA concentration was determined by UV-Vis spectrophotometry. (uwaterloo.ca)
Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. (creativebiomart.net)
Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. (creativebiomart.net)
Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. (creativebiomart.net)
Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. (creativebiomart.net)

The application of porous polymeric monolith (PPM) column as an e effective tool for bacterial cell lysis is demonstrated in this thesis. (uwaterloo.ca)
The bacterial cell wall is mechanically sheared (mechanical-shear lysis) by flowing through the narrow porous medium of the PPM column, but it is also damaged and disintegrated by physical contact (contact-killing lysis) with the antibacterial polymeric biocide covering the porous surface. (uwaterloo.ca)

Cancer is characterized by uncontrolled growth and division of a cell with extension beyond the normally limiting basement membrane and through the boundaries of normal cells. (medscape.com)

These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
LC-MS/MS analysis was used to investigate the proteome of DLD1 colon cancer cells with induced NBPF1 expression. (biomedcentral.com)
Finally, it is not known whether DR would also protect cancer cells from chemotherapy. (kingdomfasting.com)
Materials and Methods: PrP c expression was suppressed in HT29 human colon cancer cells by utilizing small-interfering RNA (si-PRNP), and cells were subsequently used to study the antiproliferative and anticancer effects of fucoidan treatment of HT29 human colon cancer cells. (iiarjournals.org)
Results: Fucoidan treatment significantly inhibited growth and reduced cyclin and cyclin-dependent kinase (CDK) expression in HT29 colon cancer cells. (iiarjournals.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
1 TKIs are now therapies used routinely in the treatment of many solid tumours such as gastrointestinal stromal tumour (GIST), renal cell carcinoma (RCC), hepatocellular carcinoma (HCC), medullary thyroid cancer, breast cancer and non-small-cell lung carcinoma (Table 1) . (touchoncology.com)
In turn, as a result of research into the abnormal cancer cell, the basic understanding of the cell has greatly improved. (medscape.com)
This is the multihit theory of tumorigenesis, in which a series of multiple triggering events in the genetic and cellular makeup of a cell ultimately cause cancer. (medscape.com)

Morphological profiling is an omics-based approach for predicting intracellular targets of chemical compounds in which the dose-dependent morphological changes induced by the compound are systematically compared to the morphological changes in gene-deleted cells. (waseda.jp)
TIF) pone.0170233.s001.tif (215K) GUID:?4A59CDD6-F306-4806-8FCD-576435A1A3FA S2 Fig: Morphological examination of HT-29 cells treated with crude hexane extract at IC25: 10.52, IC50: 21.05, and IC75:42.1 g/mL. (nanoker-society.org)
The identification and cell counting of Chattonella species have been conducted primarily on living cells without fixation by light microscopy because routine fixatives do not retain their morphological features. (e-algae.org)

These findings suggest that signaling by P2Y receptors, most likely of the purine/pyrimidine subtype, enhance the ability of FGF2 to stimulate entry into a new cell cycle, as well as DNA replication, by an ERK-dependent mechanism, whereas signaling by P2X receptors, possibly the P2X7 subtype, inhibits FGF2-induced mitogenesis in astrocytes. (sagepub.com)

Meiosis is a specialized cellular program required to create haploid gametes from diploid parent cells. (biorxiv.org)

In addition, proteome analysis of NBPF1-overexpressing DLD1 cells identified 32 differentially expressed proteins, of which several are implicated in carcinogenesis. (biomedcentral.com)
Here we identified Nup2 in a pool of enriched proteins that co-purify with tagged Ndj1 from meiotic cell extracts. (biorxiv.org)
Single-cell imaging of ATP-reduced yeast mutants revealed that ATP levels in these mutants underwent stochastic and transient depletion, which promoted the cytotoxic aggregation of endogenous proteins and pathogenic proteins, such as huntingtin and α-synuclein. (waseda.jp)

Here, we employed the used of to investigate the effectiveness of natural UCK2 inhibitors to cause HT-29 cell death. (nanoker-society.org)
There are a variety of tyrosine kinase inhibitors (TKIs) that are used in oncology for the treatment of malignancies now and consequently there have been increased observations of tumour lysis syndrome (TLS) associated with these drugs. (touchoncology.com)

Genome editing using clustered regularly-interspaced short palindromic repeats (CRISPR)/Cas9 is an easy and efficient strategy for the generation of gene-modified cells and organisms 1 , 2 . (nature.com)
By applying the random forests algorithm, a supervised machine learning approach, we show how a multi-gene signature that classifies individual cells into their correct cell cycle phase and cell size can be generated. (frontiersin.org)
No.: SR300809, USA) were used to silence the respective gene in RPMI 8226 cells. (forgetmenotinitiative.org)
On the other hand, in germline gene therapy , the egg and sperm cells of the parents are altered to be passed on to their offspring. (biotechfront.com)

Chronic myeloid leukemia (CML) is a clonal expansion of the progenitor hematopoietic stem cells arising from the existence of the fusion BCR-ABL oncogene, that represents 15%-20% of the newly diagnosed cases of leukemia patients [ 1 - 2 ]. (jcancer.org)
This, in turn, creates a clonal population of a single abnormal cell. (medscape.com)

Multiple myeloma (MM) is a malignant plasma cell disorder with debilitating symptoms related to anemia, immunosuppression, bone destruction, and renal failure. (haematologica.org)
In addition, ozone oxidizes the outer lipid layer of malignant cells and destroys them through cell lysis (break- down). (silvermedicine.org)

We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)

Abstract Uridine-cytidine kinase 2 is an enzyme that is overexpressed in abnormal cell growth and its implication is considered a hallmark of malignancy. (nanoker-society.org)

Many more investigations have been made on cells, sorted according to size, or artificially arrested at various cell cycle checkpoints. (frontiersin.org)

P. multocida has been observed invading and replicating inside host amoebae, causing lysis in the host. (wikipedia.org)
The Bovine herpes virus type 5 glycoprotein D (gD) is essential for viral penetration into host permissive cells. (bvsalud.org)
MSCs are able to evade the host cell immune system due to their low expression of major histocompatibility complex (MHC) class I molecules and complete lack MHC class II molecules and other costimulatory molecules (CD40, CD40L, CD80, and CD86) required for immune cell stimulation [ 15 - 17 ]. (hindawi.com)

However, cotransfection of multiple plasmids can cause low targeting efficiency in cells that are inefficient in DNA transfection. (nature.com)

extract (JIB extract) for melanoma cells. (medsci.org)

In hemophagocytic lymphohistiocytosis, LYST has an essential function in managing the airport growth of perforin-containing granules into secretory granules in cytotoxic Testosterone levels lymphocytes [15]. (forgetmenotinitiative.org)
The generation of hydroxyl by killer cells is critical to their cytotoxic capability. (silvermedicine.org)

We further demonstrated successful examples of multiple targeting including chromosomal deletions in human cells using the all-in-one CRISPR/Cas9 vectors constructed with our novel system. (nature.com)
Mouse FGF18 / FGF-18 derived in Human Cells. (creativebiomart.net)
In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models. (wjgnet.com)
The manifestation of is usually shown to impact lysosomal size, granule size, and autophagy in human cells [12]. (forgetmenotinitiative.org)
The transferred, expanded natural killer cells proliferated in vivo in an interleukin-2 dose-dependent fashion, persisted up to 4 weeks, were readily detectable in the human bone, inhibited myeloma growth and protected bone from myeloma-induced osteolysis. (haematologica.org)
This technologic and biologic revolution continued through the 1960s to today, and the explosion in technology has fueled the current expansion of knowledge into the working of the human cell. (medscape.com)

Premedicate Kyprolis infusions with dexamethasone prior to all Cycle 1 doses and if infusion reaction symptoms develop or reappear. (aetna.com)

Such data are only indirectly related to quantitative changes in cells at different states of division and growth. (frontiersin.org)
For the quantitative determination of rabbit OGP concentrations in serum, plasma,cell culture supernates and tissue homogenate. (antikoerper-online.de)
This ELISA kit is a solid phase ELISA designed for quantitative determination of Osteogenic Growth Peptide. (antikoerper-online.de)

Like the endogenous HPK1, both wild-type HPK1 and its kinase-dead mutant, HPK1-M46, overexpressed in Panc-1 cells, were also targeted by proteasome-mediated degradation. (aacrjournals.org)

Shi, You-Fang 2005-12-03 00:00:00 Extracellular ATP enhances the mitogenic activity of fibroblast growth factor-2 (FGF2) in astrocytes, but the molecular mechanism underlying this synergistic interaction is not known. (sagepub.com)

Ozone disrupts the integrity of the bacterial cell envelope through oxidation of the phospholipids and lipoproteins. (silvermedicine.org)

We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
The weak enzyme coatings on cells which make them vulnerable to invasion by viruses make them susceptible to oxidation and elimination from the body, which then replaces them with healthy cells. (silvermedicine.org)

This makes it an attractive on-chip lysis device that can be used in sample preparation for bio-genetics and point-of-care diagnostics. (uwaterloo.ca)
This led us to additional research the features of these genetics in myeloma cell development and success by using little interfering RNA (siRNA) strategy. (forgetmenotinitiative.org)
Because of its mutated aggressive genetics, this cell has a selective growth advantage over its neighbors. (medscape.com)

With viruses, the ozone damages the viral capsid and upsets the reproductive cycle by disrupting the virus-to-cell contact with peroxidation. (silvermedicine.org)
Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)

Then you see a number of signaling molecules that transduce signals to growth and division of these cells. (jadproce.com)

The precise spatial and temporal impact of immune cells and their cytokines on fracture healing remains obscure. (hindawi.com)
The normal process of fracture repair begins with an immediate inflammatory response as the innate immune system (macrophages, monocytes, neutrophils, and NK cells) responds with a variety of cytokines that recruit and activate several cell types, including osteoprogenitor mesenchymal stem cells (MSCs), to the site of injury [ 1 , 2 ]. (hindawi.com)

Phages replicate through two primary life cycles, the dynamics of which have important implications for their therapeutic application. (asm.org)
Coupled with reports that allogeneic MSCs have immunoprivileged status and immunomodulatory properties, there has been considerable interest in exploring the use of these cells as a therapeutic option for bone repair. (hindawi.com)

However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)

Ozone activates the Krebs cycle by enhancing oxidative carboxylation of pyruvate, stimulating production of ATP. (silvermedicine.org)
While IAEMH is associated with severe anaemia due to intravascular haemolysis caused by red cell invasion, oxidative injury, auto-antibodies, and/or pathogen-haem interaction, IAIMH is associated with haemophagocytic tri-lineage destruction of haematopoietic precursors in the bone marrow. (bvsalud.org)

1 Significant advances have been made by combining novel agents with autologous peripheral blood stem cell transplantation which allows for long-term disease-free survival in the majority of transplant-eligible patients. (haematologica.org)

A complete blood cell count (CBC) with differential should be obtained, including examination of the peripheral blood smear if the differential is abnormal. (medscape.com)
These studies suggest that multiple cycles of fasting promote differential stress sensitization in a wide range of tumors and could potentially replace or augment the efficacy of certain chemotherapy drugs in the treatment of various cancers. (kingdomfasting.com)
Diagnosis is by white blood cell count with a differential count, and evaluation requires identification of the cause. (msdmanuals.com)

Anatomy25

Organisms13

Diseases6

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes82

Disciplines and Occupations1

Information Science3

Health Care2

Inhibition12

Apoptosis13

Proliferation6

Buffer7

Mammalian3

Arrest7

Progression7

Genes11

Reagent1

Flow cytometry2

Vitro9

Protein7

Differentiation3

Regulation1

Tumor8

Toxin1

Cytometric analysis1

Neuroblastoma2

Squamous-cell carc1

Mechanisms6

Cultured in RPMI-1640 medium1

Including cell viability1

Phage6

Infection3

Strains5

Yeast cells2

Mutation2

Myeloma cells2

Vascular endoth1

Malignancy cells1

Lytic1

Lysate7

Polymeric2

Characterized by uncontrolled1

Cancer10

Morphological3

Findings1

Cellular1

Proteins3

Inhibitors2

Gene4

Clonal2

Malignant2

Subpopulations1

Abnormal cell1

Checkpoints1

Host3

Efficiency1

Melanoma1

Cytotoxic2

Human6

Dexamethasone1

Quantitative3

Kinase1

Fibroblast1

Bacterial cell1

Enzyme2

Genetics3

Viral2

Molecules1

Immune2

Therapeutic2

VEGF1

Oxidative2

Survival1

Differential3