• Hepatitis B vaccine exposure increased cell apoptosis as detected by flow cytometry and TUNEL assay. (greenmedinfo.com)
  • We conclude that exposure of Hepa1-6 cells to a low dose of adjuvanted hepatitis B vaccine leads to loss of mitochondrial integrity, apoptosis induction, and cell death, apoptosis effect was observed also in C2C12 mouse myoblast cell line after treated with low dose of vaccine (0.3, 0.1, 0.05 μg/ml). (greenmedinfo.com)
  • Our study was designed to investigate the role and related mechanisms of autophagy in matrine-induced apoptosis in hepatoma cells of HepG2 and Bel 7402 . (mdpi.com)
  • Cell apoptosis was detected by flow cytometry analysis (Annexin V-FITC/PI double-staining assay), the activity and activating cleavages of caspase-3, -8, and -9. (mdpi.com)
  • explain how prolonged stress sparks the endoplasmic reticulum (ER) to release its calcium stores, inducing cells to undergo apoptosis in several aging-related diseases.The study will appear in the September 21, 2009 issue of the Journal of Cell Biology (online September 14). (scienceblog.com)
  • In this study, we investigated the cytotoxic effects of acetaldehyde in SH-SY5Y cells and found that acetaldehyde induced apoptosis of SH-SY5Y cells by downregulating the expression of antiapoptotic Bcl-2 and Bcl-xL and upregulating the expression of proapoptotic Bax . (hindawi.com)
  • We have previously demonstrated that natural killer (NK) cells can promote apoptosis in healthy neutrophils. (frontiersin.org)
  • e.g., we have previously reported that interaction between NK cells and neutrophils can promote neutrophil apoptosis ( 6 ). (frontiersin.org)
  • Almost all hepatic cancer cells have resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis. (medscimonit.com)
  • This study investigated the role of c-FLIPL and RIP-1 in hepatic cancer cell resistance to TRAIL-induced apoptosis. (medscimonit.com)
  • Cell apoptosis was tested by flow cytometry. (medscimonit.com)
  • Single TRAIL treatment showed no significant impact on cell proliferation and apoptosis. (medscimonit.com)
  • RIP-1 or c-FLIPL siRNA markedly induced cell apoptosis and enhanced caspase-8 and caspase-3 activities. (medscimonit.com)
  • Downregulating RIP-1 and/or BAY11-7082 significantly reduced NF-kB transcriptional activity, blocked cells in G0/G1 phase, weakened proliferation, elevated caspase-8 and caspase-3 activities, and promoted cell apoptosis. (medscimonit.com)
  • Changes in cell viability, apoptosis, lactate dehydrogenase release, and cell cycle analysis were measured by cell counting kit-8 assay, annexin V/ propidium iodide staining, and enzyme-linked immunosorbent assay, respectively. (medsci.org)
  • Due to its cytotoxicity, cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells, but the specific mechanism is still unclear. (spandidos-publications.com)
  • Zhang B, Huang H, Xie J, Xu C, Chen M, Wang C, Yang A and Yin Q: Cucurmosin induces apoptosis of BxPC-3 human pancreatic cancer cells via inactivation of the EGFR signaling pathway. (spandidos-publications.com)
  • We studied autopsy specimens from has been shown to induce apoptosis in vitro ( 4 - 8 ). (cdc.gov)
  • Apoptosis modes of apoptosis induction and responsible viral genes was observed in alveolar epithelial cells, which is the major have been proposed ( 8 - 13 ). (cdc.gov)
  • However, whether play a major role in the pathogenesis of infl uenza (H5N1) and to what extent apoptosis contributes to the highly viru- virus in humans by destroying alveolar epithelial cells. (cdc.gov)
  • Progenitors are obtained by so-called direct reprogramming or directed differentiation and are also called induced somatic stem cells. (wikipedia.org)
  • This meant that the cells can change their differentiation pathway. (wikipedia.org)
  • In Drosophila imaginal discs, cells have to choose from a limited number of standard discrete differentiation states. (wikipedia.org)
  • The fact that transdetermination (change of the path of differentiation) often occurs for a group of cells rather than single cells shows that it is induced rather than part of maturation. (wikipedia.org)
  • 2012. Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks. (sigmaaldrich.com)
  • Directed Differentiation of Dopamine Neurons from Human Pluripotent Stem Cells. (sigmaaldrich.com)
  • 2009. Directed Differentiation of Human-Induced Pluripotent Stem Cells Generates Active Motor Neurons. (sigmaaldrich.com)
  • 2015. Efficient derivation and inducible differentiation of expandable skeletal myogenic cells from human ES and patient-specific iPS cells. (sigmaaldrich.com)
  • Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. (sigmaaldrich.com)
  • reported that hematopoietic stem and progenitor cells gave rise to iPSCs up to 300 times more efficiently than terminally differentiated B and T cells did, suggesting that the differentiation state of the starting cells affects reprogramming efficiency 23 . (nature.com)
  • Fe(II)/ alpha-ketoglutarate-dependent dioxygenases is dramatically potentiated by Vitamin C. iTreg differentiation and FOXP3 expression can be induced in both mouse and human CD4+ T cells by activation in the presence of TGFb and retinoic acid (RA), and we showed that addition of the TET activator Vitamin C to the culture media for iTreg generation resulted in a dramatic increase in iTreg stability and function. (nih.gov)
  • In previous studies, we showed that TET proteins are involved in cell activation and differentiation in numerous cell types in the mouse, including in early embryogenesis and embryonic stem cells, thymic differentiation, Treg and iTreg differentiation, pro-B to pre-B cell differentiation, and class switch recombination in mature B cells. (nih.gov)
  • In this supplement request, we propose to study the effect of Vitamin C on other directions of mouse and human T cell differentiation. (nih.gov)
  • We will activate naïve CD4+ T cells in culture media containing appropriate cytokines, to drive their differentiation towards four different T helper (Th) lineages: Th1, Th2, Th17 and iTreg. (nih.gov)
  • The proposed studies will enhance our understanding of a very fundamental question: how the TET activator Vitamin C alters the differentiation and function of mouse and human CD4+ and CD8+ T cells, and to what extent these effects go through TET proteins and TET enzymatic activity. (nih.gov)
  • One potential therapeutic strategy for these disorders is the manufacture of oligodendrocyte progenitor cells (OPCs) by the directed differentiation of pluripotent stem cells, including induced pluripotent stem cells (iPSCs). (nih.gov)
  • Moreover, the utility of miPSC-derived OPCs would be significantly enhanced by the adoption of a consistent, reproducible differentiation protocol that allows OPCs derived from different cell lines to be robustly characterized and compared. (nih.gov)
  • The phorbol diesters are the most potent inducers of differentiation of the promyelocytic leukemia cell line, HL-60. (jci.org)
  • The ability of a series of phorbol analogues to activate the kinase correlated with their known activity as inducers of cell differentiation. (jci.org)
  • In this work, the influence of different substrate ad hesion during phorbol-12-myristate-13-acetate (PMA)-induced differentiation of THP-1 monocytic cell line was studied. (scirp.org)
  • Type IV collagen, besides determining effects on lysosomal compart ment of intermediate and terminally differentiated cells, influenced mainly proliferative activity of cells with initial differentiation level (6 nM PMA). (scirp.org)
  • Spano, A. , Barni, S. , Bertone, V. and Sciola, L. (2013) Changes on lysosomal compartment during PMA-induced differentiation of THP-1 monocytic cells: Influence of type I and type IV collagens. (scirp.org)
  • Huh, H.Y., Pearce, S.F., Yesner, L.M., Schindler, J.L. and Silverstein, R.L. (1996) Regulated expression of CD36 during monocyte-to-macrophage differentiation: potential role of CD36 in foam cell formation. (scirp.org)
  • The study is to investigate BMSCs ability to differentiate cardiomyocytes, especially discussed cell generations and 5-Aaz concentration influence on BMSCs capability of proliferation and differentiation into cardiomyocytes during constructing the engineered myocardium-like tissue in vitro . (scirp.org)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • However, these stem/progenitor cells are generally inaccessible, require complicated procedures to obtain and are difficult to propagate in vitro . (nature.com)
  • The objective of this study was to establish an in vitro model system amenable to mechanistic investigations of cytotoxicity induced by hepatitis B vaccine, and to investigate the mechanisms of vaccine-induced cell death. (greenmedinfo.com)
  • Finally, BMDCs pulsed with HPV16 VLPs induced Th1-dominated primary T cell responses in vitro. (nih.gov)
  • Using in vitro -activated neutrophils and a human skin chamber model that allowed collection of in vivo -transmigrated neutrophils, we performed a comprehensive characterization of neutrophil expression of ligands to NK cell receptors. (frontiersin.org)
  • Ex vivo manipulation of human peripheral blood NK (pNK) cells by a combination of hypoxia, TGFß-1 and 5-aza-2'-deoxycytidine yields cells with phenotypic and in vitro functional similarities to dNK cells, called idNK cells. (harvard.edu)
  • Therefore, in the present study, the human microvascular endothelial cells (HBMECs) were exposed to oxygen-glucose deprivation/reperfusion (OGD/R) to mimic ischemic stroke condition in vitro with the aim to investigate the potential mechanisms induced by OGD/R. The permeability of cultured HBMECs was measured using FITC-labeled dextran in a Transwell system and transendothelial electrical resistance (TEER), while the RhoA activity was detected by pull-down assay. (springer.com)
  • Rat BMSCs could be differentiated into cardiomyocytes-like cells, which have a good biocompatibility with acellular bovine pericardium, and myocardium-like tissue could be engineered with BMSCs and acellular bovine pericardium in vitro . (scirp.org)
  • In conclusion, BMSCs could be induced and differentiated into cardiomyocytes-like cells in vitro . (scirp.org)
  • The mechanisms underlying the low efficiency of reprogramming somatic cells into induced pluripotent stem (iPS) cells are poorly understood," write the authors. (lifesitenews.com)
  • However, the exact mechanisms by which acetaldehyde induces neurotoxicity are not totally understood. (hindawi.com)
  • Induced pluripotent stem cells (iPSCs) offer an opportunity to delve into the mechanisms underlying development while also affording the potential to take advantage of a number of naturally occurring mutations that contribute to either disease susceptibility or resistance. (ca.gov)
  • The mechanisms of asbestos carcinogenesis remain enigmatic, as asbestos is toxic HM in cell culture. (hawaii.edu)
  • However, the underlying mechanisms involving hyperpermeability after reperfusion between the endothelial cells remain poorly understood. (springer.com)
  • Glucotoxicity and lipotoxicity are also potential mechanisms for diabetic complications, in addition to beta cell dysfunction. (medsci.org)
  • The beneficial effect of omega-3 fatty acids on atherosclerosis has been suggested to involve several mechanisms such as to induce anti-inflammatory and anti-arrhythmic effects, to improve the lipid profile by lowering triglyceride levels, to prevent plaque development, to promote plaque stabilization, and to inhibit platelet aggregation ( 6 ). (medsci.org)
  • Describe the hypothesis behind the mechanisms scientists currently believe leads asbestos to induce pathogenic changes in the lungs. (cdc.gov)
  • Other cell-mediated mechanisms (especially inflammation). (cdc.gov)
  • Other cell-mediated mechanisms (especially inflammation induced by TNF-α). (cdc.gov)
  • They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent - iMSC, also called an induced multipotent progenitor cell - iMPC) or unipotent - (iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)
  • Share your iPSC research needs on services and new iPSC lines and differentiated cell products with the European Bank for induced pluripotent Stem Cells (EBiSC). (europa.eu)
  • IMI's EBiSC project has established a centralised, not-for-profit human induced pluripotent stem cell (iPSC) bank providing researchers across academia and industry with access to scalable, cost-efficient and consistent, high quality iPSCs for use in research and medicines development. (europa.eu)
  • The new EBiSC2 project builds on the achievements of the original EBiSC project, including improving its services and providing new iPSC lines and differentiated cell products. (europa.eu)
  • Human iPSC-derived CD200 + /ITGA6 + cells are capable of generating all hair follicle lineages including the hair shaft, and the inner and outer root sheaths in skin reconstitution assays. (nature.com)
  • The technique is similar to the iPSC except the subcloned cells are not treated with the reprogramming factors which were thought to cause mutations. (genome.gov)
  • Using skin cells from the same donor, they created genetically identical copies of the cells using both the iPSC and the subcloning techniques. (genome.gov)
  • The invention of induced Pluripotent Stem Cells (iPSC) was honored by a Nobel Prize in 2012 to Dr. Shinya Yamanaka and Dr. John Gurdon whose work Dr. Yamanaka built upon. (parentsguidecordblood.org)
  • The iPSC are more like embryonic stem cells from the earliest stage of fetal development than like cord blood stem cells. (parentsguidecordblood.org)
  • The iPSC can make all the lineages of cells in a body. (parentsguidecordblood.org)
  • Equally important, scientists have shown that cord blood cells are a very good starting material for making iPSC. (parentsguidecordblood.org)
  • For instance, I Peace Inc. and Avery Therapeutics are collaborating to advance an iPSC-derived cell therapy for heart failure. (medgadget.com)
  • Hopstem Biotechnology is one of China's first iPSC cell therapy companies and a market leader in iPSC-derived clinical-grade cell products. (medgadget.com)
  • In June 2021, it partnered with Neurophth Biotechnology to co-develop an iPSC-derived cell therapy to treat ocular diseases. (medgadget.com)
  • Reprogramming of readily available blood cells promises to accelerate the utilization of iPSC technology by making it accessible to a broader research community. (jove.com)
  • Another big breakthrough on the IPSC front: Skin cells from a patient with Parkinson's-a variety caused by a genetic condition-have been turned into neural cells, and then studied as the Parkinson's destroys the tissue. (firstthings.com)
  • This latest discovery related to induced pluripotent stem cells could prove a set-back in the efforts to use them in regenerative medicine. (lifesitenews.com)
  • Ordinary human cells reprogrammed as induced pluripotent stem cells may revolutionize personalized medicine by creating new and diverse therapies unique to individual patients. (sciencedaily.com)
  • A national team of researchers, co-directed by Kun Zhang, PhD, an assistant professor of bioengineering in the UC San Diego Jacobs School of Engineering, examined 22 different hiPSC lines obtained from seven research groups that employed different methods to reprogram skin cells into pluripotent stem cells. (sciencedaily.com)
  • Working on induced pluripotent stem cells? (europa.eu)
  • What are Induced Pluripotent Stem Cells? (sigmaaldrich.com)
  • Adult somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) with the overexpression of key reprogramming genes (OCT4, KLF4, SOX2, cMYC, NANOG and LIN28). (sigmaaldrich.com)
  • Efficient Generation of Astrocytes from Human Pluripotent Stem Cells in Defined Conditions. (sigmaaldrich.com)
  • Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. (sigmaaldrich.com)
  • 2015. Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells. (sigmaaldrich.com)
  • 2010. Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells. (sigmaaldrich.com)
  • 2014. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. (sigmaaldrich.com)
  • The reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) by defined transcription factors has been a well-established technique and will provide an invaluable resource for regenerative medicine. (nature.com)
  • Induced pluripotent stem cells (iPSCs) have been generated from somatic cells by transduction of reprogramming transcription factors, such as cMYC , SOX2 , KLF4 and OCT4 . (nature.com)
  • It's been more than 10 years since Japanese researchers Shinya Yamanaka, M.D., Ph.D., and his graduate student Kazutoshi Takahashi, Ph.D., developed the breakthrough technique to return any adult cell to its earliest stage of development (a pluripotent stem cell) and change it into different types of cells in the body. (genome.gov)
  • Called induced pluripotent stem cells (iPSCs), this technique opens the doors to medical advances, including generating cartilage cell tissue to repair knees, retinal cells to improve the vision of those with age-related macular degeneration and other eye diseases, and cardiac cells to restore damaged heart tissues. (genome.gov)
  • Bathing somatic cells in acid can reprogram them to induced pluripotent stem cells, a new study found. (medindia.net)
  • A new study reveals that Joslin scientists are studying the first generation of human induced pluripotent stem cells from patients with maturity onset diabetes of the young (MODY). (medindia.net)
  • A new population of primitive, stress-resistant human pluripotent stem cells easily derived from fat tissue has been isolated by researchers from the UCLA Department of Obstetrics and Gynecology. (medindia.net)
  • Chimpanzee and bonobo skin cells have been turned them into induced pluripotent stem cells (iPSCs) by researchers at the Salk Institute for Biological Studies. (medindia.net)
  • Concise review: modeling central nervous system diseases using induced pluripotent stem cells. (ca.gov)
  • The global Induced Pluripotent Stem Cell (IPS cell) market size was valued at US$ XX billion in 2019 and is estimated to reach US$ XX billion by 2029, growing at a CAGR of XX % during the forecast period (2022-2029). (medgadget.com)
  • Induced pluripotent stem cells (iPS cell), immature cell generated from an adult (mature) cell and has regained the capacity to differentiate into any cell in the body. (medgadget.com)
  • The induced pluripotent stem cell (IPS cell) market growth is driven by the increasing therapeutic advancements with iPS Cell, growing mergers and acquisitions of organizations to expand their portfolio in iPSCs, and Increasing research activities are the major driving factors of the market. (medgadget.com)
  • The prevalence of chronic diseases worldwide is driving many vendors in the market to develop novel induced pluripotent stem cell therapies. (medgadget.com)
  • The global induced pluripotent stem cell (ips cell) market is highly competitive with presence of global companies. (medgadget.com)
  • Urea metabolism is impaired in patient-derived human-induced pluripotent stem cells (hiPSCs) differentiated into hepatocytes (hiPSC-Heps). (medicalxpress.com)
  • Salahuddin M, Sayfullah M, Hossain D, Ahmad N (2014) Ever Young: A Big Game, Induced Pluripotent Stem Cell a Positive Candidate. (omicsonline.org)
  • Induced pluripotent stem cells originate from somatic cell more specifically from patient specific somatic cell can be a possibility of this contemplation. (omicsonline.org)
  • The notion of pluripotent stem cell to replace the diseased cell is exactly similar. (omicsonline.org)
  • It is already established by some researcher that using different condition pluripotent stem cells can replace the damaged or diseases cells of the body [ 3 - 15 ]. (omicsonline.org)
  • What would be the possibilities that induced pluripotent stem cells as a candidate of ever young ? (omicsonline.org)
  • Grünenthal has expertise in developing human induced pluripotent stem cells towards sensory neurones and will support the lab of Dr Raouf with a total consideration of more than £350.000. (prleap.com)
  • Aachen, Germany and London, England, 27 April 2023 Grünenthal and King's College London announced a 24 months collaboration to develop microfluidic culture (MFC) models based on human induced pluripotent stem cells (iPSCs) and tailored to pain research. (prleap.com)
  • Induced pluripotent stem cells (iPSCs) are derived from a somatic cell that has been reprogrammed back into a pluripotent state by either introducing specific genes coding for transcription factors or adding small molecules that regulate cell identity. (prleap.com)
  • By inserting certain genes into skin cells, scientists can turn back the clock, causing them to revert to "induced pluripotent stem (iPS) cells" - with embryonic-like traits. (firstthings.com)
  • Our findings reveal a barrier for T cell-focused therapies and offer mechanistic insights into how T cell-NK cell-DC cross-talk can be enhanced to promote antitumor immunity and overcome resistance. (nih.gov)
  • The purpose of this Funding Opportunity Announcement (FOA) is to support multidisciplinary, hypothesis-driven research on Natural Killer (NK) cells, leading to the discovery of pathways relevant for early immune responses and immune regulation impacting the potential protective immunity to be induced by HIV vaccination. (nih.gov)
  • In addition to designing better immunogens, the HIV-vaccine field will likely benefit if vaccine candidates optimally engage the innate immune components that shape and improve adaptive immunity by exploiting immunological effector functions beyond those driven by B- or T-cells. (nih.gov)
  • Our data provide evidence that DCs respond to intact papillomavirus capsids and that they play a central role in VLP-induced immunity. (nih.gov)
  • CLIC1 silencing inhibited the attachment of tumor cells to culture plates and the adherence and extravasation in the lung alveoli, resulting in suppressed lung metastasis in mice. (jci.org)
  • One clue came from other recent studies suggesting that one layer of the protective covering of the brain, known as dura mater, contains immune T cells that can both secrete IL-17 and can affect the behavior of mice. (nih.gov)
  • Using special mice where cells light up fluorescent green when they make IL-17, the researchers confirmed that hypertension increases IL-17 in the dura mater which is then released into the tissue. (nih.gov)
  • However, this barrier appeared to be disrupted in the mice with experimentally induced hypertension, and this disruption allowed IL-17 to enter the cerebral spinal fluid. (nih.gov)
  • Mice homozygous for a conditional allele activated in Schwann cells exhibit transient acceleration of postnatal myelination, reduced demyelination in culture, and reduced myelin degradation and increases remyelination following nerve axotomy or sciatic nerve crush. (jax.org)
  • Mice homozygous for a null allele exhibit incomplete prenatal lethality, decreased osteoclast cell number, decreased angiogenesis, increased long bone epiphyseal plate size, and short long bones. (jax.org)
  • Senior author Ira Tabas points out the importance of understanding this mechanism further: mice lacking CHOP are protected against cell death arising from a variety of pathologies, including advanced atherosclerosis, diabetes, and neurodegeneration. (scienceblog.com)
  • Using both white blood cells isolated from the spleen of neonatal mice - the same cells used in the original study - and lung fibroblasts, Lee was unable to replicate the original findings, and has now published the full results of his study in F1000Research . (medindia.net)
  • Compared to HMGB1 wild-type mice, mesothelial cells from HMGB1-cKO mice showed significantly reduced autophagy and increased cell death. (hawaii.edu)
  • When injected into immunocompromised pregnant mice with elevated uterine artery resistance, idNK cells homed to the uterus and reduced the uterine artery resistance index, suggesting improved placental perfusion. (harvard.edu)
  • In these studies, the researchers induced skin tumorigenesis in mice by applying 7,12-dimethylbenz[ a ]anthracene (DMBA) as tumor-initiator and phorbol ester-containing croton oil as tumor-promoting agent. (frontiersin.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • Citations to Possible mechanism of phorbol diester-induced maturation of human promyelocytic leukemia cells. (jci.org)
  • Possible mechanism of phorbol diester-induced maturation of human promyelocytic leukemia cells. (jci.org)
  • These data suggest that protein kinase C is the phorbol diester receptor and that phorbol diester-induced macrophage maturation of HL-60 cells may be mediated by activation of intracellular protein kinase C. (jci.org)
  • We found that murine bone marrow-derived dendritic cells (BMDCs) effectively bound and rapidly internalized bovine papillomavirus VLPS: Exposure to fully assembled VLPs of bovine papillomavirus, human papillomavirus (HPV)16 or HPV18, but not to predominately disordered HPV16 capsomers, induced acute phenotypic maturation of BMDCS: Structurally similar polyomavirus VLPs bound to the DC surface and were internalized, but failed to induce maturation. (nih.gov)
  • Soluble phorbol diester receptors from HL-60 cells were obtained from the cytosolic fraction and from the particulate fraction by either divalent ion chelation or detergent extraction. (jci.org)
  • In collaboration with Drs. Leonid Margolis and Gregory Melikian, we have found that HIV-1 binding to its receptors induces non-apoptotic exposure of PS at the surface of the target cell and that externalized PS strongly promotes Env-mediated membrane fusion and HIV-1 infection. (nih.gov)
  • Our preliminary results suggest that during cellular transformation, Cd exposure induced endoplasmic reticulum (ER)-stress, which triggered the phosphorylation of stress transducers including PERK and eIF2-α resulted in the activation of ATF4 and initiate the induction of autophagy that protects Cd- damaged cells. (nih.gov)
  • Although, induction of autophagy markers (Atg -12 and Atg-16L, LC3B and Lamp1) were seen in Cd-treated cells, the autophagy process is incomplete, due to failure autophagosome and autolysosome fusion, which allowed the damaged cell to proliferate for transformation. (nih.gov)
  • The HMGB1 silencing inhibited autophagy and promoted asbestos-induced HM death, therefore reducing the risk of asbestos-driven HM transformation. (hawaii.edu)
  • Autophagy inhibitors chloroquine (CQ) and desmethylclomipramine (DCMI) increased cell death and reduced asbestos-led foci formation. (hawaii.edu)
  • In summary, HMGB1 released upon asbestos exposure induces autophagy, which promotes HM survival and malignant transformation. (hawaii.edu)
  • Acetaldehyde, the most toxic metabolite of ethanol, is speculated to mediate the brain tissue damage and cognitive dysfunction induced by the chronic excessive consumption of alcohol. (hindawi.com)
  • Huang Y, Tan Q, Chen R, Cao B, Li W (2015) Sevoflurane prevents lipopolysaccharide-induced barrier dysfunction in human lung microvascular endothelial cells: Rho-mediated alterations of VE-cadherin. (springer.com)
  • Wakitami, S., Saito, T. and Caplan, A.I. (1995) Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. (scirp.org)
  • In all of these cell lines, the researchers found protein-coding point mutations, an estimated six mutations per exome. (sciencedaily.com)
  • Every single stem cell line we looked at had mutations. (sciencedaily.com)
  • The interdisciplinary team at UC San Diego developed a new, highly sensitive assay to identify mutations that occur at very low frequencies in the starting cells of cell lines. (sciencedaily.com)
  • They discovered that roughly half of the mutations found in stem cell lines were present in starting cells at very low levels. (sciencedaily.com)
  • The other half of the mutations were too rare to detect in starting cells, meaning they could have occurred during or after reprogramming. (sciencedaily.com)
  • and a third group of mutations that occurred after reprogramming, when pluripotent cells began proliferating. (sciencedaily.com)
  • Despite its immense promise, adoption of iPSCs in biomedical research and medicine has been slowed by concerns that these cells are prone to increased numbers of genetic mutations. (genome.gov)
  • A new study by scientists at the National Human Genome Research Institute (NHGRI), part of the National Institutes of Health, suggests that iPSCs do not develop more mutations than cells that are duplicated by subcloning. (genome.gov)
  • They then sequenced the DNA of the skin cells as well as the iPSCs and the subcloned cells and determined that mutations occurred at the same rate in cells that were reprogrammed and in cells that were subcloned. (genome.gov)
  • Sequencing of the tumor DNA as well as further mechanistic studies provided evidence that PAHs initiate carcinogenesis by inducing mutations primarily in the Ha-Ras oncogene and that this process requires a metabolic conversion of the per se non-toxic chemicals to highly reactive metabolites, a process which is primarily carried out by AHR-regulated cytochrome P450 (CYP) monooxygenases ( 10 - 12 ). (frontiersin.org)
  • Nearly all these EGFR gene mutations occur during a person's lifetime (somatic) and are present only in cancer cells. (medlineplus.gov)
  • Somatic mutations in the EGFR gene most often occur in a type of lung cancer called non-small cell lung cancer, specifically a form called adenocarcinoma. (medlineplus.gov)
  • Lung cancers with EGFR gene mutations tend to respond to treatments that specifically target the overactive epidermal growth factor receptor protein that allows cancer cells to constantly grow and divide. (medlineplus.gov)
  • Projects generating iPSCs can also make their cell lines sustainable by depositing them in the EBiSC catalogue, ensuring secure long-term storage and simplified access and distribution. (europa.eu)
  • We offer a large collection of cell culture media, supplements, bioactive small molecules, and growth factors used to control the cell fate of human iPSCs. (sigmaaldrich.com)
  • We randomly selected six lines isolated from various developmental stages to induce iPSCs and found that five DPC lines showed higher reprogramming efficiency than human dermal fibroblasts (HDFs). (nature.com)
  • First, we attempted to generate iPSCs from DP31 cells, a cell line isolated from the immature (CC) stage of teeth, using as few transcription factors as possible. (nature.com)
  • The resulting iPSCs resemble embryonic stem cells (ESCs) in their properties and potential to differentiate into a variety of adult cell types. (nature.com)
  • Reprogramming of somatic cells into iPSCs has tremendous potential for clinical application. (nature.com)
  • However, there remain challenges in differentiating iPSCs into certain cell types. (nature.com)
  • M ost genetic variants detected in the iPSCs and subclones were rare genetic variants inherited from the parent skin cells. (genome.gov)
  • Just as with any new field, several models of screening are being explored, and innovators are working on the most efficient methods to overcome the inherent limitations of primary cell screens using iPSCs. (ca.gov)
  • Those iPSCs can be differentiated into different cell types with unique characters, including peripheral sensory neurons. (prleap.com)
  • For example, "chief" cells express the stem cell marker Troy. (wikipedia.org)
  • Immunosuppressive therapy followed by stem cell transplantation induced remission of a common form of multiple sclerosis (MS) over a 5-year follow-up period. (nih.gov)
  • A new study -- published in the March 3 issue of the journal Nature and led by scientists at the University of California, San Diego in collaboration with other leading stem cell research groups -- finds that the genetic material of reprogrammed cells may in fact be compromised, and suggests that extensive genetic screening of hiPSCs become standard practice before these stem cells are used clinically. (sciencedaily.com)
  • Goldstein is also director of the UC San Diego Stem Cell Program. (sciencedaily.com)
  • The study examined stem cell lines from many of the leading stem cell research groups in the United States, including lines from the laboratories of James Thomson at the University of Wisconsin-Madison and George Daley at the Children's Hospital Boston, the first U.S.-based labs to reprogram human cells. (sciencedaily.com)
  • Stem Cell Reports. (sigmaaldrich.com)
  • The regenerated hair follicles possess a KRT15 + stem cell population and produce hair shafts expressing hair-specific keratins. (nature.com)
  • hiPSC clones exhibiting characteristic human embryonic stem cell (hESC) morphology were isolated ~45 days after transduction ( Supplementary Fig. 1a ). (nature.com)
  • This characteristic can be triggered in other cells by transforming them into iPS cells, which further increases the potential for stem cell therapies. (medindia.net)
  • To verify these claims, Kenneth Lee set out to replicate the work in his own lab, and documented his experiments online in the process, leading to lively online discussions between stem cell scientists who provided feedback and commentary along the way. (medindia.net)
  • As we humans have developed a better understanding of the immune system and rejection responses, we have been able to develop drugs to suppress the rejection response when we perform organ transplants or stem cell transplants. (parentsguidecordblood.org)
  • Recently another type of stem cell has been manufactured by scientists. (parentsguidecordblood.org)
  • They showed that it was possible to take any cell from the body and, with a few gene engineering tricks, convert it into a stem cell. (parentsguidecordblood.org)
  • By using induced stem cell technology, we succeeded in generating liver cells that function largely like liver cells from patients," explains Dr. med. (medicalxpress.com)
  • Stem Cell Discovery , 3 , 90-98. (scirp.org)
  • Capi, O. and Gepstein, L. (2006) Myocardial regeneration strategies using human embryonic stem cell-derived cardiomyocytes. (scirp.org)
  • According to a recent study by researchers from the Lund Stem Cell Center at Lund University, published in Nature Aging, the genetic changes previously observed in aging blood stem cells could be linked to cell extraction stress rather than the aging process itself. (lu.se)
  • Researchers at Lund Stem Cell Center are particularly interested in studying hematopoietic stem cells, their changes with age, and the factors contributing to this decline. (lu.se)
  • Anna Konturek-Ciesla, a researcher at Lund Stem Cell Center and the lead author of the study explains, "In our research, which focuses on hematopoiesis, or blood cell formation, many studies have attempted to analyze the changes associated with aging in blood stem cells using gene expression profiling. (lu.se)
  • When we looked at our data, we noticed a pattern of stress-induced changes in gene activity, especially in cells exposed to higher temperatures during isolation," explains David Bryder, Professor of Molecular Hematology at Lund University, and research group leader at Lund Stem Cell Center. (lu.se)
  • Therefore, acetaldehyde induces cytotoxicity of SH-SY5Y cells via promotion of apoptotic signaling, inhibition of cell survival pathway, and induction of oxidative stress. (hindawi.com)
  • In this study, we set out to investigate how neutrophil sensitivity to NK cell-mediated cytotoxicity is regulated under inflammatory conditions. (frontiersin.org)
  • These studies revealed a dramatic downregulation of HLA class I molecules in inflammatory neutrophils, which was associated with an enhanced susceptibility to NK cell cytotoxicity. (frontiersin.org)
  • T cell-directed cancer immunotherapy often fails to generate lasting tumor control. (nih.gov)
  • Mechanistically, we demonstrate that AdV5-IL-12 is capable of inducing the expression of CC-chemokine ligand 5 (CCL5) in CD49a + NK cells both in tumor mouse models and tumor specimens from patients with cancer. (nih.gov)
  • Conversely, failure to respond to IL-12 and PD-1 blockade in tumor models with low CD49a + CXCR6 + NK cell infiltration could be overcome by intratumoral delivery of CCL5. (nih.gov)
  • Membrane protrusion and adhesion to the extracellular matrix, which involves the extension of actin filaments and formation of adhesion complexes, are the fundamental processes for cell migration, tumor invasion, and metastasis. (jci.org)
  • This study reveals what we believe is an unrecognized mechanism that spatiotemporally coordinates the formation of both lamellipodium/invadopodia and nascent cell-matrix adhesions for directional migration and tumor invasion/metastasis. (jci.org)
  • The unique traits of upregulation and membrane targeting of CLIC1 in cancer cells make it an excellent therapeutic target for tumor metastasis. (jci.org)
  • All these ROS induce tumor necrosis factor-alpha (TNF-α). (cdc.gov)
  • Lung cancer is a disease in which certain cells in the lungs become abnormal and multiply uncontrollably to form a tumor. (medlineplus.gov)
  • As a result, cells constantly receive signals to proliferate and survive, leading to tumor formation. (medlineplus.gov)
  • Hepatitis B vaccine induces apoptotic death in Hepa1-6 cells. (greenmedinfo.com)
  • Upregulation of cleaved caspase 3,7 were detected by western blot in addition to Apaf-1 and caspase 9 expressions argues that cell death takes place via the intrinsic apoptotic pathway in which release of cytochrome c from the mitochondria triggers the assembly of a caspase activation complex. (greenmedinfo.com)
  • The apoptotic machinery is triggered by calcium released from the ER, but how CHOP induces this step wasn't known. (scienceblog.com)
  • Similarly, exposure to acetaldehyde inhibits cell growth in primary cultures of rat astrocytes, presumably via apoptotic pathway [ 8 ]. (hindawi.com)
  • In addition, it has been shown that acetaldehyde induces cytotoxic effects of neuronal cells by activating apoptotic signals such as cytochrome c release and caspase 3 activation [ 13 ]. (hindawi.com)
  • We have recently demonstrated the antiproliferative and apoptotic activities of herbal traditional Chinese medicines, including the analomous fruit extract of Gleditsia sinensis, the fresh juice of Scutellaria barbata and the warmed water extract of Radix Sophorae Tonkinensis on a series of human carcinoma cells. (spandidos-publications.com)
  • Four cancer cell lines, including A549 non-small cell lung cancer, Hep3B hepatocellular carcinoma, MDA-MB231 breast cancer and SLMT-1 oesophageal squamous cell carcinoma, were incubated with BJE and strong apoptotic induction was observed under inverted microscopic investigation for all of the four cell lines tested. (spandidos-publications.com)
  • Combined transfection obviously increased apoptotic cells. (medscimonit.com)
  • We explored the function of cucurmosin in BxPC-3 pancreatic cancer cells using multiple cellular and molecular approaches such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, reverse transcription polymerase chain reaction (RT-PCR), Western blotting and transmission electron microscopy for observing typical changes and formation of apoptotic bodies. (spandidos-publications.com)
  • Numerous apoptotic enza (H5N1) virus was also shown to induce lymphopenia leukocytes were observed in the lung of a patient who died on day 6 of illness. (cdc.gov)
  • Whether observed apoptotic cells were a direct result of the viral replication or Materials and Methods a consequence of an overactivation of the immune system requires further studies. (cdc.gov)
  • After injury, mature terminally differentiated kidney cells dedifferentiate into more primordial versions of themselves and then differentiate into the cell types needing replacement in the damaged tissue Macrophages can self-renew by local proliferation of mature differentiated cells. (wikipedia.org)
  • MTT assay and colony forming assay were used to assess the effect of matrine on growth and proliferation of HCC cells. (mdpi.com)
  • The results have demonstrated that the different concentration of 5-Aza has the influence on proliferation rate of different generations of BMSCs, the second generation BMSCs was superior to the sixth, and tenth generation in proliferation capacity after being induced, and 5-Aza had some influence on proliferation capacity. (scirp.org)
  • We found that cucurmosin inhibited the proliferation of BxPC-3 cells in a time- and dose-dependent manner, and increased the cell population in the G0-G1 phase. (spandidos-publications.com)
  • As a result, signaling pathways within the cell are triggered that promote cell growth and division (proliferation) and cell survival. (medlineplus.gov)
  • The findings help answer the question of whether reprogramming adult mammalian cells into hiPSCs affects the overall genome at the fundamental level of single nucleotides. (sciencedaily.com)
  • The findings of this mouse study suggest that immune cells around and within the brain become abnormally activated under conditions that mimic a common form of hypertension, and this activation leads to impaired brain function. (nih.gov)
  • Our findings suggest that cell-surface PS acts as an important cofactor that promotes the fusogenic restructuring of pre-fusion Env-CD4-coreceptor complexes. (nih.gov)
  • We validated our findings in a novel unique mesothelial cell-specific conditional HMGB1-knockout (HMGB1-cKO) mouse model. (hawaii.edu)
  • These findings suggest that bFGF can induce IL-6 secretion in MC3T3-E1 cells through NF-κB activation. (medsci.org)
  • There is growing evidence from animal models of viral infections and also in humans that NK cells can mediate immunoregulatory and immunological memory functions, thereby significantly impacting the adaptive immune response to infection and the subsequent disease outcome. (nih.gov)
  • In rat cerebellar neuron cultures, acetaldehyde treatment causes decrease in cell viability while impairing mitochondrial function and significantly elevating markers of oxidative stress including 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine [ 12 ]. (hindawi.com)
  • However, we observed that the induced liver cells excrete significantly less urea than real, healthy liver cells, and this is independent of whether they originate from healthy controls or urea cycle patients. (medicalxpress.com)
  • FW stimulation significantly induced the secretion of basic fibroblast growth factor (bFGF) and extracellular matrix metalloproteinase inducer (EMMPRIN). (medsci.org)
  • Pull-down assay showed that RhoA activation was obviously enhanced after OGD/R treatment, while RhoA and ROCK inhibitor significantly reversed OGD/R-induced HBMEC monolayers hyperpermeability and the internalization of VE-cadherin. (springer.com)
  • In rat embryos, acetaldehyde treatment induces marked cell death in several tissues including neuroepithelium, correlating to the malformations seen in fetal alcohol syndrome (FAS) [ 7 ]. (hindawi.com)
  • Induced stem cells (iSC) are stem cells derived from somatic, reproductive, pluripotent or other cell types by deliberate epigenetic reprogramming. (wikipedia.org)
  • Asbestos causes malignant transformation of primary human mesothelial cells (HM), leading to mesothelioma. (hawaii.edu)
  • However, some asbestos-exposed HM escape cell death, where they accumulate DNA damage and may eventually become transformed. (hawaii.edu)
  • Asbestos Toxicity: How Does Asbestos Induce Pathogenic Changes? (cdc.gov)
  • The presence of asbestos fibers in the lungs sets off a variety of responses leading to inflammation, cell, and tissue damage, which can lead to malignant and non-malignant diseases. (cdc.gov)
  • Because of their surface charge, asbestos fibers can adsorb to cellular macromolecules (proteins, DNA, RNA) and cell surface proteins. (cdc.gov)
  • Binding of asbestos fibers to these cellular components is believed to induce changes in macromolecular conformation, thereby affecting macromolecule function. (cdc.gov)
  • Asbestos induces pathological changes leading to such outcomes as fibrosis and malignancy. (cdc.gov)
  • For instance, multilineage-differentiating stress-enduring (Muse) cells are stress-tolerant adult human stem cells that can self-renew. (wikipedia.org)
  • hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
  • The table below highlights the most widely used protocols, media and characterization antibodies used to differentiate human iPSC's into different cell lineages. (sigmaaldrich.com)
  • Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. (sigmaaldrich.com)
  • Here we showed that the reprogramming potential of human dental pulp cells (DPCs) obtained from immature teeth is much higher than those of mature teeth DPCs. (nature.com)
  • The hiPSC-derived CD200 + /ITGA6 + cells show a similar gene expression signature as EpSCs directly isolated from human hair follicles. (nature.com)
  • However, we have not examined any human cell types so far. (nih.gov)
  • Human Immunodeficiency virus 1 (HIV-1), the causative agent of AIDS, delivers its RNA into cells by fusing the viral envelope with the cell membrane. (nih.gov)
  • Here, gene expression profiling shows that CD56Bright idNK cells derived ex vivo from human pNK cells, and to a lesser extent CD56Dim idNK cells, are enriched in the gene expression signature that distinguishes dNK cells from pNK cells. (harvard.edu)
  • Conventional drugs have lots of side effects and also they are not pleasant for cell also drug developed in mouse trialing, is not working in many human diseases [ 2 ]. (omicsonline.org)
  • As human being we can never stop or slow down the growth of cell even cannot prevent to being aged. (omicsonline.org)
  • We used the human cervical cancer-derived fibroblastic cell line (HeLa), to examine the cytokine secretion profile following FW treatment in the present study. (medsci.org)
  • Cultured human umbilical vein endothelial cells (HUVECs) were exposed to H 2 O 2 as oxidative stress and a high glucose concentration with palmitate as a glucolipotoxic condition. (medsci.org)
  • Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment. (bvsalud.org)
  • Induced neural stem cells reprogrammed from human peripheral blood mononuclear cells , and/or thrombin plus fibrinogen , were transplanted into the lesion site of an immunosuppressed rat spinal cord injury model. (bvsalud.org)
  • Altogether, induced neural stem cells and human fibrin reduced the number of infiltrated immune cells , biased microglia towards a regenerative M2 phenotype , and changed the cytokine expression profile at the lesion site. (bvsalud.org)
  • Changes occur in all cells of the human body as we age. (lu.se)
  • Then they are coaxed into becoming mature cells, such as neurons. (firstthings.com)
  • Recent studies have mostly focused on engraftment of cells at the lesioned spinal cord , with the expectation that differentiated neurons facilitate recovery. (bvsalud.org)
  • These results offer a mechanistic explanation for the striking ability of papillomavirus VLP-based vaccines to induce potent T and B cell responses even in the absence of adjuvant. (nih.gov)
  • We aimed to examine whether EPA could protect vascular endothelial cells under stress conditions against stress-induced accelerated senescence (SIAS). (medsci.org)
  • Some types of mature, specialized adult cells can naturally revert to stem cells. (wikipedia.org)
  • The purpose of this study was to examine the effect of 9 flavors on vascular endothelial cell function. (nih.gov)
  • We conclude that a cross-talk exists between endothelial cell-derived humoral mediators and the intestinal mast cell system. (portlandpress.com)
  • Benn A, Bredow C, Casanova I, Vukičević S, Knaus P (2016) VE-cadherin facilitates BMP-induced endothelial cell permeability and signaling. (springer.com)
  • Harris ES, Nelson WJ (2010) VE-cadherin: at the front, center, and sides of endothelial cell organization and function. (springer.com)
  • Natural killer (NK) cells are cytotoxic cells that can kill aberrant cells without prior sensitization ( 7 ). (frontiersin.org)
  • Studying gene expression is essential for understanding how cells function and respond to different stimuli. (lu.se)
  • Today, performing gene expression analysis on young and aged hematopoietic stem cells is a common practice, used to understand the changes associated with aging. (lu.se)
  • Suspecting that experimental procedures used during gene expression analysis might be influencing the results, they examined how stress-related genes behaved in cells collected under different conditions. (lu.se)
  • The EGFR gene provides instructions for making a receptor protein called the epidermal growth factor receptor, which spans the cell membrane so that one end of the protein remains inside the cell and the other end projects from the outer surface of the cell. (medlineplus.gov)
  • However, the molecular mechanism underlying the malignant cell transformation following Cd exposure is yet to be determined, while the association between Cd and CaP in both pre-clinical and clinical models are well established. (nih.gov)
  • Our data indicate that HIV-cell binding triggers phosphatidylserine (PS) exposure at the cell surface. (nih.gov)
  • But if the stress continues, prolonged expression of a transcription factor called CHOP promotes cell death instead. (scienceblog.com)
  • AdV5-IL-12 imposed CCL5-induced type I conventional dendritic cell (cDC1) infiltration and thus increased DC-CD8 T cell interactions. (nih.gov)
  • To address this issue, we focused on the effect of noninfectious papillomavirus-like particles (VLPs) on dendritic cell (DC) activation. (nih.gov)
  • Meanwhile, acetaldehyde treatment caused an increase in the production of reactive oxygen species and elevated the oxidative stress in SH-SY5Y cells. (hindawi.com)
  • This positioning allows the receptor to attach (bind) to other proteins, called ligands, outside the cell and to receive signals that help the cell respond to its environment. (medlineplus.gov)
  • A similar observation was made for other IFN-γ-inducing therapies such as Programmed cell death 1 (PD-1) blockade. (nih.gov)
  • These disorders may benefit from cell-based therapies that prevent further demyelination or are able to restore lost myelin. (nih.gov)
  • Differentiated airway epithelial cells can revert into stable and functional stem cells in vivo. (wikipedia.org)
  • Epithelial stem cells (EpSCs) in the hair follicle bulge are required for hair follicle growth and cycling. (nature.com)
  • In the skin, epithelial stem cells (EpSCs) in the hair follicle contribute not only to the generation of a new hair follicle with each hair cycle but also to the repair of the epidermis during wound healing 3 , 4 . (nature.com)
  • This wound-induced unjamming transition is found to occur generically in epithelial, endothelial and cancer cells. (rsc.org)
  • Infection of epithelial cells and lymphocytes play an important role. (cdc.gov)
  • We previously demonstrated in an autopsy case that alveo- middle lung fi elds and a masslike infi ltration at the right lar epithelial cells are the major target cell type of this virus middle lung fi eld. (cdc.gov)
  • For reverse transcrip- epithelial cells (Figure 1). (cdc.gov)
  • Asano, M. EMMPRIN Inhibits bFGF-Induced IL-6 Secretion in an Osteoblastic Cell Line, MC3T3-E1. (medsci.org)
  • Here, we demonstrate that therapeutic targeting of the interferon-γ (IFN-γ)-interleukin-12 (IL-12) pathway relies on the ability of a population of natural killer (NK) cells with tissue-resident traits to orchestrate an antitumor microenvironment. (nih.gov)
  • Thus, therapeutic efficacy depends on the abundance of NK cells with tissue-resident traits and, specifically, their capacity to produce the DC chemoattractant CCL5. (nih.gov)
  • In newts, muscle tissue is regenerated from specialized muscle cells that dedifferentiate and forget the type of cell they had been. (wikipedia.org)
  • First, the research team generated liver cells from patients' skin tissue in an elaborate process. (medicalxpress.com)
  • Microfluidic devices are compartmentalised chips consisting of different chambers, sometimes called ´lab on a chip´ or 'tissue chips', allowing cell-to-cell contact via a series of connecting channels. (prleap.com)
  • The effects of endothelin-1 (ET-1) on mucosal mast cells are of special interest, since they may be an important component of the tissue response that occurs during ischaemic preconditioning or ischaemia/re-oxygenation injuries. (portlandpress.com)
  • With systematically collected and fully available data, Lee and his colleagues report that carefully replicating the original acid-treatment method does not induce pluripotency in two types of mouse somatic cells, including those used in the original study. (medindia.net)
  • The mouse liver hepatoma cell line Hepa1-6 was treated with two doses of adjuvanted (aluminium hydroxide) hepatitis B vaccine (0.5 and 1 μg protein per ml) and cell integrity was measured after 24, 48 and 72 h. (greenmedinfo.com)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)
  • In a next step, the researchers developed a process in which the formation of aquaporin 9 is induced in the stem cells . (medicalxpress.com)
  • Lund University researchers find that individual blood stem cells (depicted as snowflakes in this image) express stress-related transcripts when subjected to experimental procedures involving cell incubation at elevated temperatures. (lu.se)
  • One promising treatment for MS is HDIT/HCT (high-dose immunosuppressive therapy with autologous hematopoietic cell transplant). (nih.gov)
  • The treatment involves first collecting a patient's hematopoietic stem cells (HSCs)-precursor cells that develop into blood cells. (nih.gov)
  • Hematopoietic stem cells, known as blood stem cells, are crucial for producing all types of blood cells throughout a person's life. (lu.se)
  • Despite this wealth of knowledge, Anna Konturek-Ciesla noted that there is still no consensus about the molecular profile of aged hematopoietic stem cells. (lu.se)
  • ERO1-alpha is a transcriptional target of CHOP, and its reexpression in cells lacking CHOP restored the cell death pathway. (scienceblog.com)
  • A CRISPR screen identifies a pathway required for paraquat-induced cell death. (broadinstitute.org)
  • Using the MDA-MB231 breast cancer cell line as an experimental model, additional analyses supported the hypothesis that the mitochondrial membrane potential depolarization pathway was induced by BJE. (spandidos-publications.com)
  • Collectively, BJE-induced cancer cell death proceeds through a mitochondrial dependent pathway associated with caspase 3 activation. (spandidos-publications.com)
  • Cellular senescence is a process in which various stressors permanently stop cell division, resulting in cell changes that appear as senescent-associated secretory phenotype (SASP) ( 2 ). (medsci.org)
  • On direct questioning about her history of blood disorders, the patient recalled being told that she had sickle cell trait, but denied ever having a sickle cell crisis. (medscape.com)
  • TY - JOUR T1 - A study of induced hyponatremia in the prevention and treatment of sickle-cell crisis. (unboundmedicine.com)
  • Neutrophils are potent effector cells and contain a battery of harmful substances and degrading enzymes. (frontiersin.org)
  • In response to migration-related stimuli, CLIC1 recruited PIP5K1A and PIP5K1C from the cytoplasm to the leading edge of the plasma membrane, where PIP5Ks generate a phosphatidylinositol 4,5-bisphosphate-rich (PIP2-rich) microdomain to induce the formation of integrin-mediated cell-matrix adhesions and the signaling for cytoskeleon extension. (jci.org)
  • The transmigration process per se is dependent on degranulation, which enables attachment to endothelial cells, and subsequently chemotaxis-driven migration ( 2 ). (frontiersin.org)
  • Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
  • The destruction of the blood-brain barrier (BBB) contributes to a spectrum of neurological diseases such as stroke, and the hyperpermeability of endothelial cells is one of the characters of stroke, which is possibly exacerbated after reperfusion. (springer.com)
  • Cutaneous squamous cell carcinoma (SCC) is one of the most frequent malignancies in humans and academia as well as public authorities expect a further increase of its incidence in the next years. (frontiersin.org)
  • Cell monolayers provide an interesting example of active matter, exhibiting a phase transition from flowing to jammed states as they age. (rsc.org)
  • Its means the cells of their body will never turns to senescence or the aged cells turn again into juvenile stage. (omicsonline.org)
  • Also we cannot return the aged cell into young cell, but we can introduce some way that the senescence cell are somehow replace by young cell time to time. (omicsonline.org)
  • Scientists reason that they would have a better chance of replicating the disorder with her cells, rather than the cells of someone with a more sporadic form of the disease. (firstthings.com)
  • The goal of this application is to investigate the underlying mechanism of how Cd causes malignant cell transformation (from normal to transformed cells) and on the development of tumorigenesis by the Cd-transformed cells (transformed cells to tumorigenesis). (nih.gov)
  • Because the source of paraquat-induced ROS production remains unknown, we conducted a CRISPR-based positive-selection screen to identify metabolic genes essential for paraquat-induced cell death. (broadinstitute.org)
  • Our screen uncovered three genes, POR (cytochrome P450 oxidoreductase), ATP7A (copper transporter), and SLC45A4 (sucrose transporter), required for paraquat-induced cell death. (broadinstitute.org)
  • Similarly, we will activate naive CD8+ T cells in IL-2-containing culture media so that they differentiate to become cytolytic effector T cells. (nih.gov)
  • While patients would have to take immune suppression to prevent rejection, the cost of making the cells and distributing them would be cheap when done on a mass scale. (parentsguidecordblood.org)
  • In three patients with sickle-cell anemia who had been disabled by recurrent painful crises, sustained dilutional hyponatremia was induced by 1-desamino-8-D-arginine vasopressin (DDAVP) in combination with a high fluid intake. (unboundmedicine.com)
  • And it is impossible to extract cells from deep inside the brains of patients. (firstthings.com)
  • By the time patients die, the brain cells are dead, too. (firstthings.com)
  • Here, we aimed to investigate the role of microenvironment modulation by cell graft on functional recovery after spinal cord injury . (bvsalud.org)
  • Prockop, D.J. (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues. (scirp.org)
  • A new study finds that the genetic material of reprogrammed cells may in fact be compromised, and suggests that extensive genetic screening of hiPSCs become standard practice. (sciencedaily.com)