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Hematopoietic Stem CellsHematopoietic Stem Cell TransplantationStem CellsHematopoiesisStem Cell TransplantationStem Cell NicheCell DifferentiationHematopoietic Stem Cell MobilizationTransplantation, HomologousCell LineageAntigens, CD34Adult Stem CellsEmbryonic Stem CellsBone Marrow CellsColony-Forming Units AssayStem Cell FactorPluripotent Stem CellsMice, Inbred C57BLMultipotent Stem CellsGraft vs Host DiseaseFlow CytometryTransplantation ConditioningBone MarrowFetal BloodCells, CulturedBone Marrow TransplantationCell ProliferationTransplantation, AutologousCell SeparationHematopoietic SystemHematologic NeoplasmsMesonephrosInduced Pluripotent Stem CellsMesenchymal Stromal CellsNeural Stem CellsMesenchymal Stem Cell TransplantationMice, SCIDFetal Stem CellsTransplantation ChimeraProto-Oncogene Proteins c-kitCord Blood Stem Cell TransplantationMice, Inbred NODWhole-Body IrradiationGraft SurvivalLentivirusCell Culture TechniquesTransduction, GeneticChimerismLeukemiaMyeloid Progenitor CellsGranulocyte Colony-Stimulating FactorPeripheral Blood Stem Cell TransplantationTissue DonorsRetroviridaeLymphopoiesisGenetic VectorsAntigens, CDMice, KnockoutHematopoiesis, ExtramedullaryNeoplastic Stem CellsMice, TransgenicRadiation ChimeraTime FactorsCell DivisionStromal CellsSignal TransductionAntigens, CD38Antigens, LyThrombopoietinRegenerationBusulfanEmbryo, MammalianGenetic TherapyHistocompatibility TestingStem Cell ResearchHistocompatibilityLymphoid Progenitor CellsCoculture TechniquesReverse Transcriptase Polymerase Chain ReactionAntigens, Thy-1Hematologic DiseasesPlant StemsTreatment OutcomeMyeloid CellsYolk SacChemokine CXCL12Green Fluorescent ProteinsMyelopoiesisPhenotypeCell CountSevere Combined ImmunodeficiencyMice, CongenicCell SurvivalBlood CellsMyelodysplastic SyndromesGene Expression Regulation, DevelopmentalCell CycleLeukemia, Myeloid, AcuteGonadsGene Expression Regulation
TransplantationHSCsProgenitorsEngraftmentHSCTDonorProgenitor cellDifferentiationReconstitutionTransplantsMyeloidBlood cell lineagesMiceHematopoiesisProliferationPatientsTissueCD34Peripheral blood stem cellsCord bloodRegenerationReplenishTransplant recipientsHSPCs2022LymphocytesTissuesFatesSickle cell diClinicalUmbilicalMarrowPopulationsVitroCitationEndothelial cellsAdultSCIDGraftHeterogeneityBiologyInfusionAssaysMarkersCancerGene
Transplantation59
HSCs32
  • Hematopoietic stem cells (HSCs) are the stem cells that give rise to other blood cells. (wikipedia.org)
  • In vertebrates, the very first definitive HSCs arise from the ventral endothelial wall of the embryonic aorta within the (midgestational) aorta-gonad-mesonephros region, through a process known as endothelial-to-hematopoietic transition. (wikipedia.org)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
  • Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
  • We found that HSCs from Lnk-/- mice have an increased quiescent fraction, decelerated cell cycle kinetics, and enhanced resistance to repeat treatments with cytoablative 5-fluorouracil in vivo compared with WT HSCs. (jci.org)
  • Lnk -/- LSK HSCs show decelerated cell cycle kinetics. (jci.org)
  • Haematopoietic stem cells (HSCs) are a self-renewing population of cells that continuously replenish all blood and immune cells during the lifetime of an individual. (ca.gov)
  • HSCs are used clinically to treat a wide array of diseases, including acute leukaemias and congenital blood disorders, but obtaining suitable numbers of cells and finding immune-compatible donors remain serious problems. (ca.gov)
  • These difficulties have led to an interest in the conversion of embryonic stem cells or induced pluripotent stem cells into HSCs, which is not possible using current methodologies. (ca.gov)
  • Notch signalling downstream of Dlc and Dld is earlier than, and distinct from, known cell-autonomous requirements for Notch, strongly suggesting that novel Notch-dependent relay signal(s) induce the first HSCs in parallel to other established pathways. (ca.gov)
  • Bone marrow hematopoietic stem cells (HSCs) are vital for the maintenance of hematopoiesis. (irbbarcelona.org)
  • In inbred mice housed in gnotobiotic facilities, the top of the hematopoietic hierarchy is occupied by dormant HSCs, which reversibly exit quiescence during stress. (irbbarcelona.org)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
  • To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
  • Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. (duke.edu)
  • Steady-state hematopoietic stem cells' (HSCs) self-renewal and differentiation toward their mature progeny in the adult bone marrow is tightly regulated by cues from the microenvironment. (elsevierpure.com)
  • Here, we review emerging evidence showing how HSCs and their progeny are regulated by an interdependent network of mesenchymal stromal cells, nerve fibers, the vasculature, and also other hematopoietic cells. (elsevierpure.com)
  • FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
  • Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
  • HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)
  • Moreover, we observed molecular changes in cell cycle, MYC and mTOR signaling in both HSCs, and progenitor subsets. (lu.se)
  • The finding that MYCT1 is downregulated in hematopoietic stem cells (HSCs) and required for their engraftment in immunodeficient mice, as determined by the Mikkola lab, raises a crucial question about how MYCT1 contributes to the "stemness" of HSCs. (ucla-mls.com)
  • Hematopoietic stem cells (HSCs), first produced in the developing vertebrate embryo, supply the lifelong foundation of the blood and immune systems. (grantome.com)
  • Likewise, while the number of transplanted HSCs is well known to directly impact engraftment efficiency, there are currently no established clinical protocols to successfully expand donor-harvested HSCs, nor to differentiate embryonic or induced pluripotent stem cells (iPSCs) into functional HSCs in vitro. (grantome.com)
  • In the developing embryo, definitive HSCs arise from a unique population of mesodermal precursors termed hemogenic endothelium (HEC) through a process known as endothelial-to-hematopoietic transition (EHT). (grantome.com)
  • Hematopoietic stem cells (HSCs), produced in the developing embryo, form the foundation of our blood system. (grantome.com)
Progenitors9
  • The hematopoietic tissue contains cells with long-term and short-term regeneration capacities and committed multipotent, oligopotent, and unipotent progenitors. (wikipedia.org)
  • Left panels show representative FACS plots of Lin - Kit hi BM cells gated for LSK and LK HSC progenitors. (jci.org)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • 02:14 progenitors that make red blood cells. (yale.edu)
  • They found that islet1 -expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche (Figure 1A). (go.jp)
  • In this study, we tried to elucidate signal transduction pathway in primitive hematopoietic progenitors of mice stimulated with interleukin-11(IL-11). (nii.ac.jp)
  • First, effects of calphostin C, a specific inhibitor for protein Kinase C(PKC), and genistein and herbimycin A, specific tyrosine kinase (TK) inhibitors on colony formation of 5-FU resistant, dormant, primitive hematopoietic progenitors supported by IL-6 which receptor has the same signal transducer gp 130 of IL-11 receptor and 12-o-tetradecanoyl phorbol-13-acetate(TPA). (nii.ac.jp)
  • These findings suggested that signal of IL-11 in colony formation of primitive hematopoietic progenitors was transduced through an activation of PKC and TK.Confirmation of these findings by immunocytochemical examination on activation of PKC and TK by IL-11 is on going. (nii.ac.jp)
Engraftment7
  • In contrast to the expected mixed chimerism that usually occurs in the absence of conditioning, we found in our patients 100% donor cell engraftment based on fluorescence in situ hybridization (FISH) and microsatellite techniques. (nih.gov)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • Incomplete engraftment (poor graft function) requires the patient to undergo more transfusions of red cells or platelets. (drugdiscoverynews.com)
  • One trial is testing whether MSCs with or without peripheral blood stem cells could treat poor graft function and delayed platelet engraftment. (drugdiscoverynews.com)
  • Early reports indicated cell engraftment from bone marrow transplants into non- hematopoietic tissues in mouse to mouse and in human allogeneic bone marrow transplants. (ucl.ac.uk)
  • The study, which involved 130 patients, also found that adding fludarabine to conditioning caused three-fold enhancement of hematopoietic cell engraftment. (medscape.com)
HSCT9
  • Cells for HSCT may be obtained from the patient himself or herself (autologous transplant) or from another person, such as a sibling or unrelated donor (allogeneic transplant) or an identical twin (syngeneic transplant). (medscape.com)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • Among the conditions HSCT can treat are: acute myeloid leukemia, acute lymphoblastic leukemia, chronic myeloid leukemia, chronic lymphocytic leukemia, myeloproliferative disorders, myelodysplastic syndromes, multiple myeloma, non-Hodgkin lymphoma, Hodgkin disease, aplastic anemia and pure red-cell aplasia-but this list is not exhaustive. (drugdiscoverynews.com)
  • Other groups are also working on cell therapies for the treatment of poor graft function after HSCT. (drugdiscoverynews.com)
  • Their ongoing Phase 2 study is evaluating whether infusion of mesenchymal stem cells (MSCs) can treat steroid-resistant acute graft-versus-host disease (GVHD) or poor graft function after HSCT. (drugdiscoverynews.com)
  • Peptide mixes were utilised that cover the CMV antigens pp65 and IE1 to study the functionality of CD4+ and CD8+ T cells in patients post HSCT. (ucl.ac.uk)
  • The data presented here demonstrates the importance of a CD4+ T cell response to CMV in the HSCT setting and also highlights the benefit of both a CD8+ T cell responses to both IE1 and pp65. (ucl.ac.uk)
  • Thus, we studied the safety, efficacy, and feasibility of transfer of gene modified donor T-cells shortly after allo-HSCT in two clinical trials between 2002 and 2007 and here we compare the results to unmodified donor leukocyte infusion (DLI). (frontiersin.org)
  • The aim of these trials was to provide patients with the protection of T-cells after T-cell-depleted allo-HSCT in the matched or mismatched donor setting with an option to delete transduced T-cells, if severe aGvHD occurred within the trial period. (frontiersin.org)
Donor12
  • Subset analysis of the engrafted cells using a multiparametric system enabling a combined analysis of morphology, immunophenotyping and FISH showed that both T and B lymphocytes and myeloid cells were of donor origin in two patients, while T lymphocytes and myeloid cells were of donor origin in the third. (nih.gov)
  • Understanding how hematopoietic stem cells are made during embryonic development is important because it could teach us how to make such cells in the laboratory, and possibly allow circumvention of donor immune compatibility issues. (ca.gov)
  • We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
  • Majority of patients (97%) were taking newer tri-azole prophylaxis (posaconazole) as per standard centre protocol.Results: Pre-IFD cases were similar to those without pre-IFD in gender, age, disease type, stem cell source, conditioning regimen and donor source whereas conditioning intensity, donor matching and HCTCI scores were significantly different. (kcl.ac.uk)
  • In this procedure, stem cells from bone marrow, blood, or umbilical cord blood are taken from another person (donor) and implanted into the patient. (newbornscreening.info)
  • Donor-T-cells were transduced with the replication-deficient retrovirus SFCMM-3, expressing HSV-TK and the truncated ΔLNGFR for selection of transduced cells. (frontiersin.org)
  • Donor chimerism was stabilized after transfusion of the transduced cells in all patients treated. (frontiersin.org)
  • Thus, prophylactic transfusion of donor T-cells has been included in many protocols, despite the increased risk for acute GvHD ( Kolb, 2008 ). (frontiersin.org)
  • Stem cells are removed from another person, called a donor. (medlineplus.gov)
  • Leukapheresis -- First, the donor is given several days of shots to help stem cells move from the bone marrow into the blood. (medlineplus.gov)
  • The red blood cells are returned to the donor. (medlineplus.gov)
  • Here, we report transmission of dengue virus to a peripheral blood stem cell recipient by a donor who had recently traveled to an area to which the virus is endemic. (cdc.gov)
Progenitor cell4
  • We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
  • We observed enhanced progenitor cell activity within the CD34+ cell population treated with Delta1 in hypoxia, but the benefits provided by low-oxygen cultures were most notable in the primitive HSC compartment. (confex.com)
  • Scholars@Duke publication: Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. (duke.edu)
  • This review outlines current protocols and cell markers used in flow cytometric analysis of hematopoietic stem and progenitor cell populations . (bvsalud.org)
Differentiation10
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCultâ„¢ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • In hematopoietic tissues, a group of ECs are highly specialized and constitute the vascular HSPC niche to support HSPC maturation, expansion, and differentiation. (go.jp)
  • B lood cell differentiation begins with multipotent hematopoietic progenitor cells (HPCs), which are located in the marrow spaces of the bone. (nationalacademies.org)
  • These primitive cells undergo division and differentiation to form the various peripheral blood cells. (nationalacademies.org)
  • The adult bone marrow provides essential niches for hematopoietic stem cell (HSC) maintenance and differentiation, which are not fully understood. (elsevierpure.com)
  • Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
  • They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
  • Specific niches within the bone marrow are discussed, as are metabolic processes that contribute to stem cell self-renewal and differentiation, as well as the role of hematopoietic stem cells outside of the bone marrow at physiologic steady state . (bvsalud.org)
Reconstitution1
  • These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo. (duke.edu)
Transplants4
  • These rare cells are the important element of bone marrow transplants, which are used to treat a variety of conditions including many forms of blood cancer. (ca.gov)
  • There are many risks associated with stem cell transplants. (newbornscreening.info)
  • 0.001).DiscussionThe use of AHSCT in people with active SPMS is associated with a slowing of disability progression and a higher likelihood of disability improvement compared with standard immunotherapy.Classification of EvidenceThis study provides Class III evidence that autologous hematopoietic stem cell transplants prolonged the time to CDP compared with other DMTs. (mssm.edu)
  • Haematopoietic stem cell transplants have been performed in more than 1 500 000 patients (both autologous and allogeneic) to date.1 Although haematological cancers remain the main indication, haematopoietic stem cell transplants are increasingly considered in the treatment of non-malignant disorders and genetic diseases such as haemoglobinopathies (sickle cell anaemia, thalassaemia) that can benefit greatly from this type of transplant. (who.int)
Myeloid15
  • Hematopoietic stem cells give rise to different types of blood cells, in lines called myeloid and lymphoid. (wikipedia.org)
  • Myeloid and lymphoid lineages both are involved in dendritic cell formation. (wikipedia.org)
  • Hematopoietic stem cells constitute 1:10,000 of cells in myeloid tissue. (wikipedia.org)
  • All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
  • These factors may regulate myeloid versus lymphoid balance with age, and can potentially mitigate the long-term deleterious effects of inflammation that lead to hematopoietic pathologies. (biorxiv.org)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Physiologic aging in both humans and mice leads to permanent changes in LT-HSC function, such as myeloid-biased hematopoietic output ( Akunuru and Geiger, 2016 ). (biorxiv.org)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
  • Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • We develop a computational model for the hematopoietic erythroid-myeloid lineage decision, which is determined by a genetic switch involving the genes PU.1 and GATA-1. (lu.se)
  • Citation: Chickarmane V, Enver T, Peterson C (2009) Computational Modeling of the Hematopoietic Erythroid-Myeloid Switch Reveals Insights into Cooperativity, Priming, and Irreversibility. (lu.se)
  • However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)
Blood cell lineages2
  • HSPCs are multipotent cells that can self-renew and differentiate to give rise to all blood cell lineages. (go.jp)
  • Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
Mice3
  • Another CFU, the colony-forming unit-spleen (CFU-S), was the basis of an in vivo clonal colony formation, which depends on the ability of infused bone marrow cells to give rise to clones of maturing hematopoietic cells in the spleens of irradiated mice after 8 to 12 days. (wikipedia.org)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
  • BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. (duke.edu)
Hematopoiesis6
Proliferation2
  • While progenitor cells can divide and replicate, their proliferation potential is more restricted compared to stem cells. (thenoveldifference.com)
  • Figure 2: Manipulating stem cell osteogenesis and proliferation by controlling the elasticity of the bulk phase of void-forming hydrogels. (nature.com)
Patients8
  • Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
  • Pluristem Therapeutics, based in Haifa, Israel, is currently recruiting patients in the United States and Israel for its Phase 1 trial of PLX-R18 cells in this unmet need. (drugdiscoverynews.com)
  • The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. (hindawi.com)
  • There was no detectable CD4+ T cell response to CMV lysate in patients that had detectable CMV replication, additionally the CD8+ T cell responses against pp65 and IE1 was not adequate to prevent CMV replication in the absence of a CD4+ T cell response. (ucl.ac.uk)
  • The data presented here shows that these patients have a high number of T cells that respond to the CMV antigens IE1 and pp65, indicating that these patients do experience CMV reactivations resulting in persistent antigenic stimulation. (ucl.ac.uk)
  • Under immunosuppression conditions, all infectious foci can be activated and, for this reason, all active infections in hematopoietic stem cell pre-transplant patients must be eradicated prior to transplant so as to prevent or reduce the risk of systemic complications in these patients. (bvsalud.org)
  • Hematopoietic cell transplant (HCT) can cause significant distress in patients and their informal caregivers. (open.ac.uk)
  • A study by Tang et al determined that long-term TKI therapy may reduce the abundance of leukemic stem cells in select patients. (medscape.com)
Tissue10
  • An infectious retrovirus vector has been used to transfer a bacterial gene encoding resistance to the neomycin analogue G418 into pluripotent haematopoietic stem cells present in explanted murine bone marrow tissue. (nature.com)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • They also serve as specialized vascular niches that provide instructive signals to tissue-specific stem cells or parenchymal cells for tissue formation and repair. (go.jp)
  • When these islet1 -lineage ECs were specifically ablated, most of the HSPCs were lost from the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs in zebrafish (Figure 1B). (go.jp)
  • islet1 + -derived ECs (green) are mainly located in the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs. (go.jp)
  • To achieve true transdifferentiation a different, more chronic type of tissue damage, or a better defined stem cell population might be required. (ucl.ac.uk)
  • Progenitor cells play a crucial role in tissue repair and regeneration. (thenoveldifference.com)
  • When specific tissue or organ damage occurs, these cells can rapidly generate new cells of the desired type to facilitate the healing process. (thenoveldifference.com)
  • Progenitor cells are being explored for various therapeutic applications, including tissue engineering and regenerative medicine. (thenoveldifference.com)
  • They are committed to a specific cell lineage but retain the ability to generate a limited range of related cell types, making them essential for tissue repair, regeneration, and potential medical interventions. (thenoveldifference.com)
CD345
  • citation needed] Hematopoietic stem cells can be identified or isolated by the use of flow cytometry where the combination of several different cell surface markers (particularly CD34) are used to separate the rare Hematopoietic stem cells from the surrounding blood cells. (wikipedia.org)
  • They received megadoses of stem cells (18-23 x 10(6)CD34/kg). (nih.gov)
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • CD34-selection of stem cells reduces the risk of aGvHD, but also leads to increased infectious complications and relapse. (frontiersin.org)
Peripheral blood stem cells1
Cord blood5
  • To investigate the developmental potential of human stem cells I used xenotransplantation of stem cells purified from cord blood into a mouse model. (ucl.ac.uk)
  • Umbilical cord blood is a rich source of these committed progenitor cells and, presumably, multipotent HPCs (Knudtzon, 1974). (nationalacademies.org)
  • Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments. (wakehealth.edu)
  • One concern about the procedure is that immune cells in cord blood do not have experience with the viruses responsible for latent infections, leading to a higher percentage of naive T cells and thus increased vulnerability to reactivation of cytomegalovirus or Epstein-Barr virus infection. (msdmanuals.com)
  • Umbilical cord blood cells are very immature so there is less of a need for perfect matching. (medlineplus.gov)
Regeneration2
Replenish2
  • Hematopoietic stem cells can replenish all blood cell types (i.e., are multipotent) and self-renew. (wikipedia.org)
  • Here, they reside in specialized niches where they remain quiescent until needed to replenish and repair the blood cell population. (thenoveldifference.com)
Transplant recipients1
HSPCs3
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
  • Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (go.jp)
20222
Lymphocytes2
  • In shape, hematopoietic stem cells resemble lymphocytes. (wikipedia.org)
  • The cell mediated immune response notably that mediated through cytotoxic T lymphocytes (CTL) is essential in the maintenance of viral latency and the resolution of primary infections. (ucl.ac.uk)
Tissues3
  • However, little is known about EC heterogeneity in hematopoietic tissues. (go.jp)
  • Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
  • They offer the potential to replace damaged or dysfunctional cells in specific tissues or organs. (thenoveldifference.com)
Fates2
  • Genetic dissection of mesodermal commitment to the hematopoietic fates. (ca.gov)
  • Homozygous genetic deletion of Taspase1 disrupted embryonic hematopoietic stem cell self-renewal and quiescence states and axial skeleton fates. (jci.org)
Sickle cell di1
  • Despite advances in supportive therapy to prevent complications of sickle cell disease (SCD), access to care is not universal. (duke.edu)
Clinical1
  • Each of those cell sources has specific advantages and disadvantages, and each has found particular clinical applications. (medscape.com)
Umbilical2
  • The very first hematopoietic stem cells during (mouse and human) embryonic development are found in aorta-gonad-mesonephros region and the vitelline and umbilical arteries. (wikipedia.org)
  • Stem cells are removed from a newborn baby's umbilical cord right after birth. (medlineplus.gov)
Marrow16
Populations1
  • Like all stem cell populations, they become increasingly dysfunctional with age , however. (fightaging.org)
Vitro5
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • 02:47 and red blood cells in vitro. (yale.edu)
  • Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. (nature.com)
  • Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
  • Figure 3: Controlling cell deployment kinetics from void-forming hydrogels in vitro and in vivo . (nature.com)
Citation1
  • It was used extensively in early studies, but is now considered to measure more mature progenitor or transit-amplifying cells rather than stem cells[citation needed]. (wikipedia.org)
Endothelial cells4
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
  • The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
  • Researchers identify the origin of endothelial cells that constitute the vascular niche for hematopoietic stem and progenitor cells in zebrafish. (go.jp)
Adult2
  • At present the research community is progressing towards this goal one stem cell population at a time, but it seems plausible that some discoveries will be broadly applicable to all stem cells in the adult body. (fightaging.org)
  • Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
SCID1
  • Developmental potential of human haematopoietic stem cells in the NOD/Scid xenotransplantation model. (ucl.ac.uk)
Graft1
  • Although T-cell depletion (TCD) reduces the risk of GVHD after a myeloablative conditioning regimen, it is associated with an increased risk of graft failure. (qub.ac.uk)
Heterogeneity1
  • Here we review the currently known cellular and molecular components of the niche for hematopoietic stem cells and their progeny, highlight their heterogeneity and interdependency, and discuss some open questions in the field. (elsevierpure.com)
Biology1
  • 2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
Infusion1
  • Stem cell infusion is a relatively simple process that is performed at the bedside. (medscape.com)
Assays1
  • In this webinar, CFC assay expert Dr. Jackie Damen will describe how customized hematopoietic CFC assays can be applied to assist you in the successful development of your small molecule compounds and biologics. (stemcell.com)
Markers3
  • Lack of expression of lineage markers is used in combination with detection of several positive cell-surface markers to isolate hematopoietic stem cells. (wikipedia.org)
  • However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
  • Instead human cells most probably of haematopoietic origin fuse with resident hepatocytes to give rise to mixed heterokaryons expressing some, but not all markers of human hepatocytes. (ucl.ac.uk)
Cancer3
  • Simpson RJ, Bigley AB, Agha N, Hanley PJ, Bollard CM. Mobilizing Immune Cells With Exercise for Cancer Immunotherapy. (ctsicn.org)
  • Ablative (myeloablative) treatment -- High-dose chemotherapy, radiation, or both are given to kill any cancer cells. (medlineplus.gov)
  • For many cancers, the donor's white blood cells may attack any remaining cancer cells, which are seen as foreign, similar to when white cells attack bacteria or viruses when fighting an infection. (medlineplus.gov)
Gene3
  • To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
  • To allow better identification of human cells transduction with a lentivirus carrying the GFP gene was used. (ucl.ac.uk)
  • Functionality of HSV-TK gene expressing T-cells was shown by loss of bcr-able gene expression as well as by control of cytomegalovirus-reactivation. (frontiersin.org)