Cells antigens cd11b. Medical search. Frequent questions

Interestingly, the most primordial defense responses employed by myeloid cells against pathogens, such as complement activation, antibody-dependent cell cytotoxicity and phagocytosis, actually seem to favor cancer progression. (frontiersin.org)
antibody responses to polysaccharide antigens are decreased and antibody responses to protein antigens are slightly reduced. (medscape.com)
dashed line histograms) or BD Horizon™ BV711 Rat Anti-CD11b antibody (Cat. (bdbiosciences.com)
Flow cytometry surface staining pattern of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC). (exbio.cz)
Separation of murine leukocytes (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC). (exbio.cz)
therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. (bdbiosciences.com)
Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. (bdbiosciences.com)
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
We can help you create your own custom cell separation kit or you can combine your own antibody with our EasySep™ indirect kits. (stemcell.com)
Combine your own mouse IgG1 antibody with our EasySep™ "Do-it-Yourself" Positive Selection Kits to isolate the cells you want. (stemcell.com)
The BM8.1 antibody is specific for mouse F4/80 antigen, a 125 kDa transmembrane protein widely expressed by members of the mononuclear phagocyte system and considered to be a key marker for mature macrophage cells. (cytekbio.com)
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)

The majority of blister-originated chemotactic signals were associated with preferential recruitment of CD45 + CXCR2 + and CD11b + CXCR2 + leukocytes. (biomedcentral.com)
We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)

However, these immunotherapeutic approaches are hampered by the tumor-promoting nature of myeloid cells, including monocytes, macrophages, and neutrophils. (frontiersin.org)
Myeloid innate immune cells, such as granulocytes, monocytes, macrophages and dendritic cells (DCs), play an important role in cancer-cell recognition, initiation of inflammation and antitumor responses ( 2 ). (frontiersin.org)
CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. (biolegend.com)
Granulocytes and macrophages can be identified by the intense brown labeling of their cell surface membranes (40x magnification). (bdbiosciences.com)
Mice with a conditional deletion of FURIN in their myeloid cells (LysMCre- fur (fl/fl) ) were healthy and showed unchanged proportions of neutrophils and macrophages. (oncotarget.com)
In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. (jci.org)
However, the role of recruited monocyte-derived macrophages, including TAM, as potential cross-presenting cells is not well understood. (jci.org)
MHC II-positive macrophages are a primary target of infection and a crucial effector cell controlling parasite growth, yet their function as antigen-presenting cells remains controversial. (silverchair.com)
Importantly, B cells, macrophages, and LCs are all MHC II-negative in these mice. (silverchair.com)
Leishmania major is an intracellular protozoan that infects and multiplies within macrophages, although it can also infect Langerhans cells (LCs), DCs, and neutrophils ( 1 , 2 ). (silverchair.com)
MHC II-positive B cells, macrophages, and DCs accumulate in draining LNs of resistant mice ( 3 ) and class II-deficient (A β b−/− ) mice cannot control the infection ( 4 ). (silverchair.com)
METHODOLOGY/PRINCIPAL FINDINGS: Using animal models of breast cancer metastasis, we show that a population of host macrophages displaying a distinct phenotype is recruited to extravasating pulmonary metastatic cells regardless of species of origin. (ox.ac.uk)
Furthermore, imaging of intact lungs revealed that macrophages are required for efficient tumor cell extravasation. (ox.ac.uk)
CONCLUSION/SIGNIFICANCE: These data indicate a direct enhancement of metastatic growth by macrophages through their effects on tumor cell extravasation, survival and subsequent growth and identifies these cells as a new therapeutic target for treatment of metastatic disease. (ox.ac.uk)
CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. (abeomics.com)
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. (abeomics.com)
Repetitive DE treatment for 3 weeks resulted in significant increases in CD11c(+)/CD11b(+) macrophages in whole lung-associated tissue. (cdc.gov)
These studies demonstrate that DE elicits activated CD11c(+)/CD11b(+) macrophages in the lung, which play a critical role in regulating the outcome of DE-induced airway inflammation. (cdc.gov)

A combination of CD34/CD117/CD13/CD16 staining showed that VSTM1-v1 expression in myeloid cells was positively correlated with cell maturation state. (biomedcentral.com)

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. (biolegend.com)
CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. (biolegend.com)

CD18 non-covalently associates with CD11a, CD11b or CD11c. (angioproteomie.com)
BM8.1 is widely used together with antibodies to CD115 (c-fms), CD11b and CD11c to identify myeloid / macrophage cells by flow cytometry. (cytekbio.com)
Three CD11 alpha chains and a common CD18 beta chain form heterodimer transmembrane complexes (CD11a/CD18, CD11b/CD18, CD11c/CD18). (medscape.com)
CD11a/CD18 is also known as leukocyte factor antigen-1 (LFA-1), CD11b/CD18 is known as CR3, and CD11c/CD18 is known as CR4. (medscape.com)
Ligands for CD11a/CD18 are intercellular adhesion molecules (ICAMs), ligands for CD11b/CD18 are complement C3bi and ICAMs, and ligands for CD11c/CD18 are C3bi and ICAMs. (medscape.com)
CD18 deficiency results in loss of expression of LFA-1, CR3 (CD11b/CD18), and CR4 (CD11c/CD18) (see Table 3). (medscape.com)
CD11c(+)/CD11b(+) cells are critical for organic dust-elicited murine lung inflammation. (cdc.gov)
Similar findings were observed with the CD11c(+)/CD11b(+) macrophage infiltrate after repetitive exposure to peptidoglycan, a major DE component. (cdc.gov)

Mucosal LCs can be segregated into CD103(+)CD11b(lo) (CD103(+)) and CD11b(+)CD103(-) (CD11b(+)) subsets. (nih.gov)
This includes the expression of distinct antigens on subsets of MSCs, which was utilized recently to define and separate functionally different subsets from bulk MSCs. (hindawi.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. (thermofisher.com)
We demonstrate that antigen presentation by these DC subsets is sufficient to control a subcutaneous L. major infection. (silverchair.com)
Other cell types shown to express this antigen include Langerhans cells, Kupffer cells and dendritic cell subsets. (cytekbio.com)
In the case of critical COVID-19, accelerating activation of some inflammatory processes contributes to exhaustion of specific lymphocyte subsets and promotes cell death pathways ( Paludan & Mogensen, 2022 ). (life-science-alliance.org)

[ 8 ] Deficiency of CD18 on phagocytic cells causes LAD type 1 (see Table 2). (medscape.com)
CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). (exbio.cz)
CD18 and associated a chains function in adhesion and signaling in hematopoietic cells. (angioproteomie.com)
Here, we show that the blood protein fibrinogen induces spine elimination and promotes cognitive deficits mediated by CD11b-CD18 microglia activation. (stanford.edu)
expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. (abeomics.com)
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Generation of recombinant fragments of CD11b expressing the functional β-glucan-binding lectin site of CR3 (CD11b/CD18). (southernbiotech.com)

These cells include dermal resident dendritic cells (DC), epidermal Langerhans cells, neutrophils and monocytes, which are released from the blood when the dermal capillary is disrupted and a reputed blood lake is formed [ 3 - 6 ]. (biomedcentral.com)
It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. (exbio.cz)
e.g., dendritic cells, monocytes and B lymphocytes) in minimally manipulated whole blood samples. (ox.ac.uk)
CONCLUSION: DPSS can induce apoptosis through cell cycle arrest, inhibit the proliferation of K562 cells, and induce K562 cells to differentiate into monocytes, which has a potential anti-leukemia effect. (bvsalud.org)
These fractions consisted of either mononuclear cells (MO-MDSCs), resembling inflammatory monocytes, or low-density polymorphonuclear cells (PMN-MDSCs), akin to immature neutrophils. (ashpublications.org)

PRR on myeloid cells can be triggered by pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs), but also indirectly by secondary mechanisms such as complement activation and circulating antibodies (Abs), resulting in cytolytic and phagocytic effector mechanisms. (frontiersin.org)
Simultaneous detection of up to eight colors is enabled by careful selection and testing of cell-subset-defining monoclonal antibodies (anchor markers) in the appropriate fluorochrome combinations, in order to show the quantification of surface expression levels of molecules involved in chemotaxis (e.g. (ox.ac.uk)
Leverage our more than 20 years of cell separation expertise , extensive range of antibodies, and variety of cell isolation methods to create tailor-made products to isolate your cell type of interest. (stemcell.com)
Use your own conjugated antibodies with EasySep™ Release kits or EasySep Biotin, FITC, PE or APC selection kits to isolate your cell of interest. (stemcell.com)

CR2 associates with CD19 forming a CR2-CD19 complex when stimulated by C3d-bearing antigen engaging CR2. (medscape.com)

Leukocyte differentiation antigens often represent important markers for the diagnosis, classification, prognosis, and therapeutic targeting of myeloid leukemia. (biomedcentral.com)
Herein, we report a potential leukocyte differentiation antigen gene VSTM1 ( V-set and transmembrane domain-containing 1 ) that was downregulated in bone marrow cells from leukemia patients and exhibited a higher degree of promoter methylation. (biomedcentral.com)
Therefore, VSTM1-v1 might represent an important myeloid leukocyte differentiation antigen and provide a potential target for the diagnosis and treatment of leukemia. (biomedcentral.com)
VSTM1 ( V-set and transmembrane domain-containing 1 ) encodes a potential leukocyte differentiation antigen that is highly expressed in myeloid cells, but silenced in multiple leukemia cell lines [ 1 ]. (biomedcentral.com)
1997. The Leukocyte Antigen FactsBook Academic Press. (elabscience.com)
These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)

There was no consistent decrease in the migration of CD11b + F4/80 + (macrophage) or SSC hi GR-1 + (neutrophil) populations. (biomedcentral.com)
Clearance of L. major in resistant C57Bl/6 mice requires IL-12-driven Th1 CD4 + T cells and macrophage activation for the killing of intracellular amastigotes ( 2 ). (silverchair.com)
A distinct macrophage population mediates metastatic breast cancer cell extravasation, establishment and growth. (ox.ac.uk)
These defects lead to abnormal neutrophil, macrophage, and T-cell and B-cell adhesion to vascular endothelium and subsequent migration into infectious sites. (medscape.com)
Macrophage infiltrates are increased in the lungs after organic dust exposures, yet the phenotype and functional importance of these cells remain unclear. (cdc.gov)
8. Ritelli M, Amadori M, Tagliabue S, Pacciarini ML. Use of a macrophage cell line for rapid detection of Mycobacterium bovis in diagnostic samples. (southernbiotech.com)

Cell differentiation antigen, CD33 and CD11b, were positively expressed after treated with DPSS. (bvsalud.org)

Furthermore, closed head injury was associated with a delayed increase in the expression of the immune cell surface glycoprotein Mac-1 (CD11B antigen) at the injury site that was decreased in NAP-treated mice. (nih.gov)

Multiparameter flow cytometric analysis of CD11b expression on mouse bone-marrow cells. (bdbiosciences.com)

We further demonstrated that similar to non-lymphoid dendritic cells (DCs), CD103(+) LCs originate from pre-DCs, whereas CD11b(+) LCs differentiate from both pre-DCs and monocytic precursors. (nih.gov)
Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)
This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)

This suggests that unique tissue-specific factors (including local lipid antigens, cytokines and/or hormones) may shape the population of iNKT cells resident in those tissues, ultimately regulating local immune responses. (elifesciences.org)
Despite this prototypical TCR repertoire gene usage, in recent years it has become apparent that there are variations within the iNKT cell repertoire that ultimately impact the antigen recognition capacity and consequently the functional outcomes during an immune response. (elifesciences.org)
Over the past decade, cancer immunotherapy has been steering immune responses toward cancer cell eradication. (frontiersin.org)
Immune cells abundantly infiltrate tumors, creating a complex environment mediated by repetitive cycles of antitumor responses and immune evasion ( 1 ). (frontiersin.org)
Hence, myeloid cells play a dual role in cancer as they can initiate antitumor responses and communicate with cells of the adaptive immune system, but also promote local inflammation leading to chronic cancer-associated inflammation ( 5 , 6 ). (frontiersin.org)
The innate immune response by myeloid cells occurs as a succession of events starting at signaling through cytosolic or surface PRRs, followed by effector responses including the release of cytokines, reactive oxygen species (ROS), reactive nitrogen species (RNS), antibacterial peptides and degranulation ( Figure 1 ). (frontiersin.org)
In rats orally exposed for one week to E171 at human relevant levels, titanium was detected in the immune cells of Peyer's patches (PP) as observed with the TiO 2 -NP model NM-105. (nature.com)
In all TiO 2 -treated rats, stimulation of immune cells isolated from PP showed a decrease in Thelper (Th)-1 IFN-γ secretion, while splenic Th1/Th17 inflammatory responses sharply increased. (nature.com)
Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)
In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
Together, iMC expand independent of the type of anti-tumor response and are not immune-suppressive in a cell-autonomous fashion. (mdc-berlin.de)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
FURIN is upregulated in activated immune cells and it regulates T-cell dependent peripheral tolerance and the Th1/Th2 balance. (oncotarget.com)
Acquired immune responses are initiated by activation of CD4 + helper T (Th) cells via recognition of antigens presented by conventional dendritic cells (cDCs). (life-science-alliance.org)
DCs instruct Th-cell polarization program into specific effector Th subset, which will dictate the type of immune responses. (life-science-alliance.org)
These findings reveal that Runx/Cbfβ complexes play crucial and complementary roles in cDCs and Th cells to shape converging type 3 immune responses. (life-science-alliance.org)
Conventional dendritic cells (cDCs) are specialized antigen-presenting cells of the immune system. (life-science-alliance.org)
Thus, fibrinogen-induced spine elimination and cognitive decline via CD11b link cerebrovascular damage with immune-mediated neurodegeneration and may have important implications in AD and related conditions. (stanford.edu)
The induction of CD11b + Gr-1 + myeloid-derived suppressor cells (MDSCs) is an important immune-evading mechanism used by tumors. (ashpublications.org)
Inflammasome signaling is a reaction cascade that influences immune response and cell death. (thno.org)
This was accompanied by comparable profiles of infiltrating immune cells into the lungs of infected mice. (life-science-alliance.org)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

Also, XCR1+ DCs are related to CD103+CD11b- DCs. (wikipedia.org)
Here, we show that loss of Runx/Cbfβ transcription factors complexes during DC development leads to loss of CD103 + CD11b + cDC2s and alters characteristics of CD103 − CD11b + cDCs in the intestine, which was accompanied with impaired differentiation of Rorγt + Th17 cells and type 3 Rorγt + regulatory T cells. (life-science-alliance.org)
However, gut CD103 + DCs are now subdivided into CD103 + CD11b + cDC2 and CD103 + CD11b − cDC1. (life-science-alliance.org)

To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)

C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6c (GR-1) Pacific Blue™ and CD11b (clone M1/70) Brilliant Violet 750™ (left) or Ly-6G/Ly-6C (Gr-1) Pacific Blue™ only (right). (biolegend.com)
Then the section was stained with 5 µg/ml of CD11b (clone M1/70) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective. (biolegend.com)
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 594 (filled histogram). (biolegend.com)

Dendritic cell frequency increased in PP regardless of the TiO 2 treatment, while regulatory T cells involved in dampening inflammatory responses decreased with E171 only, an effect still observed after 100 days of treatment. (nature.com)
Tumors frequently induce immature myeloid cells (iMC), which suppress specific and unrelated cytotoxic T lymphocyte (CTL) responses and are termed myeloid derived suppressor cells (MDSC). (mdc-berlin.de)
Regardless of the model, tumors produced IL-6 and VEGF but not GM-CSF and induced iMC (CD11b(+)Gr-1(int)) that suppressed CTL responses in vitro. (mdc-berlin.de)
None of the iMC from the different tumor models suppressed CTL responses in adoptive cell transfer experiments, unless GM-CSF was provided in vivo. (mdc-berlin.de)
His work covers various topics, including B cell responses to viral infection and vaccination, human primary immunodeficiency, and biology of lymphocyte development and function and to elucidate etiology of immunological disorders. (stanford.edu)
Although the exact roles of Rorγt + type 3 Treg cells have not yet been unraveled, they are involved in suppressing exaggerated Th2 responses ( Ohnmacht et al, 2015 ), Th17 and Th1 responses ( Sefik et al, 2015 ). (life-science-alliance.org)
Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T cell responses is crucial for antitumor immunity. (jci.org)
Similarly, infected Langerhans cells (LCs) can prime interferon (IFN)γ-producing Th1 CD4 + T cells, but whether they are required for Th1 responses is unknown. (silverchair.com)
Trained immunity of natural killer (NK) cells has shown great potential in the treatment of cancers by eliciting enhanced effector responses to restimulation by cytokines or cancer cells for long time periods after preactivation. (cancerbiomed.org)
Interestingly, both MO-MDSCs and PMN-MDSCs suppressed antigen-specific T-cell responses, albeit using distinct effector molecules and signaling pathways. (ashpublications.org)

However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
Overview of studies published regarding MSCs using the term "stem cell" or "stromal cell" in the last 20 years accessed by a web search in July 2015 (Google Scholar). (hindawi.com)
It seems that the term "stem cell" became more popular although the "stemness" was only shown in a more strict sense for MSCs involved in osteogenesis and bone repair. (hindawi.com)
When the biological properties of MSCs were explored in more detail, questions arose whether these cells met the criterion of a true stem cell [ 8 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
Over the past few years, MSCs researches have achieved some inspiring results and some of which moved up to clinic period from preclinical phases, resulting in the marketing approval of a few cell-based therapy products (CTPs) by different national regulatory authorities [18]. (researchsquare.com)
We established a Good Manufacturing Practice (GMP) grade cell facility to produce clinic-grade human umbilical cord derived MSCs (HUCMSCs) for treating premature ovarian failure (POF) and recurrent uterine adhesion [18, 20]. (researchsquare.com)
MSCs played a crucial role in regenerative therapy and have been introduced as an interdisciplinary field between cell biology and material science. (techscience.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)

Tissue homeostasis is critically dependent on the function of tissue-resident lymphocytes, including lipid-reactive invariant natural killer T (iNKT) cells. (elifesciences.org)

Despite the arsenal of defense strategies against foreign invaders, myeloid cells succumb to the instructions of an established tumor. (frontiersin.org)
In this review, we discuss how rudimentary defense mechanisms deployed by myeloid cells can promote tumor progression. (frontiersin.org)
Nevertheless, the majority of scientific discoveries points toward a more tumor-supporting role for myeloid cells in the TME. (frontiersin.org)
The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of lymphoid (ie, low-to-moderate side light-scatter-gated events, Left Panel) and myeloid cells (ie, moderate-to-high side light-scatter-gated events, Right Panel). (bdbiosciences.com)
Therefore, we speculated that VSTM1-v1 expression might be associated with the maturity of myeloid cells. (biomedcentral.com)

Langerhans cells (LCs) populate the mucosal epithelium, a major entry portal for pathogens, yet their ontogeny remains unclear. (nih.gov)

However, despite encouraging results, these trials revealed that the majority of curative effects resulted from the secretion of various paracrine factors elicited by transplanted cells rather than from donation of the therapeutic protein to the affected cutaneous tissue [ 11 ]. (biomedcentral.com)
Inflammasomes refer to the cytoplasmic multimeric protein complexes that sense pathogen- or danger-associated molecular patterns (PAMPs or DAMPs, respectively) to mediate the inflammatory response and induce programmed cell death known as pyroptosis [ 1 ]. (thno.org)
SARS-CoV-2 enters into cells using the spike protein (S), which is cleaved into the S1 and S2 subunits, by TMPRSS2 or furin-like enzymes, depending on the sequence of spike at the S1/S2 junction. (life-science-alliance.org)
it occurs when mannose-binding lectin (MBL), a serum protein, binds to mannose, fucose, or N -acetylglucosamine groups on bacterial cell walls, yeast walls, or viruses. (msdmanuals.com)

Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). (thermofisher.com)
Control of the intracellular protozoan, Leishmania major , requires major histocompatibility complex class II (MHC II)-dependent antigen presentation and CD4 + T cell T helper cell 1 (Th1) differentiation. (silverchair.com)

In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
CD8α + and CD11b + DCs can be infected in vitro ( 6 ), and T cell priming to an immunodominant L. major peptide is mediated by CD11b + DCs ( 7 ). (silverchair.com)

Although tumor-promoting functions have been attributed to other granulocytes, like eosinophils ( 17 ), basophils ( 18 ) and mast cells ( 19 ), further research is required to fully elucidate their role in cancer, as antitumoral roles have also been described ( 20 , 21 ). (frontiersin.org)
However, the tumor microenvironment (TME) imposes different mechanisms that facilitate the impairment of DC functions, such as inefficient antigen presentation or polarization into immunosuppressive DCs. (mdpi.com)
In the first but not the two latter models, tumors induced CTL hypo-responsiveness to tumor unrelated antigens. (mdc-berlin.de)
To confirm the presence of cross-presenting TAM in vivo, we performed phenotypic and functional analysis of TAM from B16-F10 and CT26 syngeneic tumor models and have identified CD11b+F4/80hiCD206+ TAM to effectively cross-present TAA. (jci.org)
We show that CD11b+CD206+ TAM represent the dominant tumor-infiltrating myeloid cell population, expressing a unique cell surface repertoire, promoting Ag cross-presentation and Ag-specific CD8+ T cell activation comparable with cross-presenting CLEC9A+ DCs (cDC1). (jci.org)
5. Force WR, Glass AA, Benedict CA, Cheung TC, Lama J, Ware CF. Discrete signaling regions in the lymphotoxin-β receptor for tumor necrosis factor receptor-associated factor binding, subcellular localization, and activation of cell death and NF-κB pathways. (southernbiotech.com)

At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
Hence, it is important to unravel how differentiation and/or activation of DC are linked with Th-cell-intrinsic mechanism that directs differentiation toward a specific effector Th subset. (life-science-alliance.org)
However, the class II-positive APCs required for CD4 + T cell priming, effector differentiation, and parasite control have not been identified. (silverchair.com)

When mice lose the ability to express XCL1, they are deficient in accumulation of tDCs and producing naturally occurring regulatory T cells (nT reg cells). (wikipedia.org)
Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. (thermofisher.com)

Naive CD8+ T cells are prepared when tumors form by cross-presentation via XCR1+ DCs and as a result will require a lower threshold to respond to antigen. (wikipedia.org)
In addition to TAMs, solid tumors are also infiltrated by immunosuppressive, immature myeloid progenitor cells, commonly referred to as monocytic or polymorphonuclear myeloid-derived suppressor cells (M/PMN-MDSC) ( 11 - 13 ). (frontiersin.org)
Effective immunotherapy of stromal-rich tumors requires simultaneous targeting of cancer cells and immunosuppressive elements of the microenvironment. (aacrjournals.org)

Pathogen clearance is mediated by mechanisms such as phagocytosis, respiratory burst with the production of ROS and RNS and release of bacteriostatic peptides, but also through the cell-extrinsic initiation of inflammation via the release of proinflammatory cytokines and chemokines ( 24 ). (frontiersin.org)
F4/80 is differentially expressed during myeloid cell development, and may be regulated by certain cytokines within the tissue microenvironment. (cytekbio.com)

This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)

By analysing iNKT cells from lymphoid tissues of mice and humans we demonstrate that their T cell receptor (TCR) repertoire is highly diverse and is distinct for cells from various tissues resulting in differential lipid-antigen recognition. (elifesciences.org)
Evidence suggests that C1q binds a receptor present on phagocytic cells, termed C1qRP. (medscape.com)

[ 3 ] Thus, it enhances and prolongs antigen signaling on B cells. (medscape.com)
The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. (exbio.cz)
CD40 ligand trimer enhances the response of CD8 + T cells to Mycobacterium tuberculosis. (southernbiotech.com)

We also show that a Runx-binding enhancer in the Rorc gene is essential for T cells to integrate cDC-derived signals to induce Rorγt expression. (life-science-alliance.org)

Only some of these CD8+ murine cells expressed XCR1 receptors. (wikipedia.org)
Cross presentation of XCR1+ CD8+ and XCR1+ CD8- cells was strongest, as is expected since they have XCR1 receptors. (wikipedia.org)
These receptors are present on phagocytic cells, mast cells, and lung epithelial and smooth muscle cells. (medscape.com)

Yet, if and how the tissue environment shapes the antigen specificity of iNKT cells remains unknown. (elifesciences.org)

The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
Fibrinogen-induced spine elimination was prevented by inhibiting reactive oxygen species (ROS) generation or genetic ablation of CD11b. (stanford.edu)

They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
The key mechanism that regulates recruitment of leukocytes and progenitor stem cells to distal anatomical tissues affected with disease is chemotaxis, which depends on the signaling molecules, chemokines, and acts primarily as part of the host defense and repair mechanism. (biomedcentral.com)

Within peripheral tissues iNKT cell recent thymic emigrants exhibit a different TCR repertoire than mature cells, suggesting that the iNKT population is shaped after arrival to the periphery. (elifesciences.org)
Since iNKT cell activation can prevent or promote immunopathology in diverse disease contexts, the strict control of peripheral iNKT cell homeostasis is vital to regulate local immunity. (elifesciences.org)
Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. (justia.com)
In particular, embodiments of the method involve inhibition of both Rac1 and Rac2 GTPases to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
The researchers used whole-exome sequencing to detect the presence of clonal hematopoiesis of indeterminate potential in peripheral blood cells and associated that discovery with coronary heart disease using samples from 4 case-control studies that together enrolled 4,726 participants with coronary heart disease and 3,529 controls. (ascopost.com)
Figure 1: Surface staining of PMA-activated human peripheral blood cells with anti-human CD11b activation epitope (CBRM1/5) PE. (abeomics.com)

Here we report application of human umbilical cord mesenchymal stem cell (HUCMSC)-derived therapy for pulmonary arterial hypertension (PAH). (nature.com)
A comparative analysis of single-cell RNA sequencing data collected from three HUCMSCs and two human umbilical vein endothelial cell (HUVEC) controls identified eight common cell clusters, all of which indicated regenerative potential specific for HUCMSCs. (nature.com)
Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry. (ox.ac.uk)
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10 6 cells in a suspension. (abeomics.com)
However, the human NK cells responsible for the generation and maintenance of trained immunity are largely unknown. (cancerbiomed.org)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
Postinternalization inhibition of adenovirus gene expression and infectious virus production in human T-cell lines. (southernbiotech.com)
10. Mongini PK, Inman JK, Han H, Kalled SL, Fattah RJ, McCormick S. Innate immunity and human B cell clonal expansion: effects on the recirculating B2 subpopulation. (southernbiotech.com)

CD4+ and CD8+ may become outdated terms, since the activity of the cell appears to be primarily dependent upon the expression of XCR1, which will make a population far more similar than the expression of CD4 or CD8. (wikipedia.org)
iNKT cells have been traditionally defined by the expression of an invariant TCR α-chain (Vα14-Jα18 in mice or Vα24-Jα18 in humans) and their capacity to recognise the glycolipid antigen α-galactosylceramide (αGalCer) presented on CD1d. (elifesciences.org)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
The expression level of its predominant encoded product, VSTM1-v1, was positively correlated with myeloid cell maturation state. (biomedcentral.com)
Restoration of VSTM1-v1 expression inhibited myeloid leukemia cells' growth. (biomedcentral.com)
To determine whether it plays a role in leukemogenesis, we characterized its expression pattern and function in bone marrow cells from AML/CML patients and myeloid leukemia cell lines. (biomedcentral.com)
We measured VSTM1 expression in leukemia cell lines and bone marrow biopsies from leukemia patients using qRT-PCR. (biomedcentral.com)
We explored the antigen-presenting cell requirement during primary L. major infection using a mouse model in which MHC II, I-A β b , expression is restricted to CD11b + and CD8α + dendritic cells (DCs). (silverchair.com)
These cells displayed increased costimulatory molecule (CD80 and CD86) expression, enhanced phagocytic ability, and an increased production of IL-6, CXCL1, and CXCL2. (cdc.gov)

BALB/c mouse bone-marrow cells were stained with either BD Horizon™ BV711 Rat IgG2b, κ Isotype Control (Cat. (bdbiosciences.com)

Such changes in dendritic cell migration may be associated with immunological events that maintain inflammation at the sites of infection. (biomedcentral.com)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
In many cases, successfully mobilization is not effected in the patient and inadequate numbers of hematopoietic stem cells are harvested from these patients using current methods. (justia.com)
Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
The molecular mechanisms that govern directional migration of the adult stem cells, allowing their efficient and controlled homing to the skin affected with DEB, are poorly understood. (biomedcentral.com)
Systemic transplantation of an enriched CXCR2 population of mouse adipose-derived stem cells (mADSC) into DEB-affected mice demonstrated effective recruitment of cells to the blistering skin under the influence of blister-derived ligands and deposition of therapeutic type VII collagen. (biomedcentral.com)
Collectively, these studies demonstrate that recruitment of mADSC into DEB skin is tightly controlled by disease-site chemotactic activities and suggest a potential mechanism for effective application of therapeutic stem cells for DEB. (biomedcentral.com)
Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. (bvsalud.org)
Results: there are five main sources of stem cells of dental origin: stem cells from dental pulp of permanent teeth and deciduous teeth, apical papilla, periodontal ligament and dental follicle. (bvsalud.org)
Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)

Cross-presenting dendritic cells (DCs) in the spleen develop into XCR1+ DCs in the small intestine, T cell zones of Peyer's patches, and T cell zones and sinuses of mesenteric lymph nodes. (wikipedia.org)

When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
The properties of HUCMSCs were validated by untargeted label-free quantitation of the cell and CM proteome, suggesting increased activity of regeneration, autophagy and anti-inflammation pathways and mitochondrial function. (nature.com)

XCL1 contributes to chemotaxis only in CD8+ murine cells, but not other DC types, B cells, T cells, or NK cells. (wikipedia.org)

Here, using immunocytochemistry, we localized the increase in ADNP to microglia and astrocyte-like cells. (nih.gov)

Anatomy32

Organisms9

Diseases4

Chemicals and Drugs157

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes31

Disciplines and Occupations1

Information Science3

Health Care1

Antibody14

CD452

Macrophages18

CD341

Integrin2

CD11c8

Subsets7

CD188

Monocytes5

Antibodies4

CD191

Leukocyte6

Macrophage6

Differentiation antigen1

Glycoprotein1

Flow cytometric analysis1

Differentiate6

Immune23

CD1033

Mesenchymal stem7

Clone3

Responses10

MSCs22

Lymphocytes1

Myeloid cells5

Langerhans1

Protein4

Intracellular2

Vitro2

Tumor6

Effector3

Regulatory2

Tumors3

Cytokines2

Endothelial cells1

Receptor2

Enhances3

Induce1

Receptors3

Specificity1

Ablation2

Progenitor8

Peripheral7

Human9

Expression9

Isotype1

Dendritic cell1

Stem cells20

Lymph nodes1

Inflammation2

Murine1

Microglia1