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  • genes
  • Dynamic repositioning of genes in the nucleus of lymphocytes preparing for cell division // Mol. (nsc.ru)
  • Association of transcriptionally silent genes with Ikaros complexes at cen-tromeric heterochromatin // Cell. (nsc.ru)
  • By molecular separation of transposons, from a cell nucleus, the cloning is enabled for genes which contain the transposons. (wikipedia.org)
  • Hundreds and thousands of genes are misregulated in cloned animals," Dr. Jaenisch said. (toledoblade.com)
  • The stem cell field's conventional wisdom suggests that it should have been some other signalling genes instead," said corresponding author of the research, Dr Ng Shyh-Chang. (innovations-report.com)
  • Eggan's research goals at Harvard were to understand how nuclear transplantation works, and to make stem cells that carry genes for specific diseases such as Parkinson's disease, amyotrophic lateral sclerosis (Lou Gehrig's disease), and Alzheimer's. (wikipedia.org)
  • Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. (wikipedia.org)
  • Herbert Boyer and Stanley Cohen selectively clone genes in bacteria, using bacterial plasmids cut by specific endonucleases (1975). (wikipedia.org)
  • A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
  • world's
  • On November 2015, a Chinese biotech company Boyalife Group announced that it will partner with Hwang's laboratory, Sooam Biotech, to open the world's largest animal cloning factory in Tianjin as early as 2016. (wikipedia.org)
  • pigs
  • Aberrant methylation of porcine OPN was frequently found in different tissues of somatic nuclear transferred cloning pigs, and bisulfite sequence data suggested that the OPN promoter region −2615 to −2239 nucleotides (nt) may be a crucial regulation DNA element. (hindawi.com)
  • These data suggested that methylation in the OPN promoter plays a crucial role in the regulation of OPN expression that we found in cloned pigs genome. (hindawi.com)
  • It also considered the extent to which current knowledge on the cloning of cattle and pigs could be applied to sheep, goats and chickens. (europa.eu)
  • biology
  • We now believe that the potential to make a normal human baby with cloning is almost zero," said Dr. Rudolf Jaenisch, professor of biology at the Massachusetts Institute of Technology, at a genetics conference at Maine's Jackson Laboratory. (toledoblade.com)
  • The word "clone" has several different meanings in biology. (encyclopedia.com)
  • 2007. Interspecies nuclear transfer: implications for embryonic stem cell biology. (koreascience.or.kr)
  • Kevin Eggan (born 1974 in Normal, Illinois) is a Professor of Stem Cell and Regenerative Biology at Harvard University, known for his work in stem cell research (also known as "therapeutic cloning"), and as a spokesperson for stem cell research in the United States. (wikipedia.org)
  • In August 2004, Eggan moved to Harvard University in Cambridge, Massachusetts, as a junior fellow, becoming an assistant professor of Molecular & Cellular Biology at their Stem Cell Institute ("HSCI") in 2005. (wikipedia.org)
  • However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single stranded RNA or DNA production and a host of other molecular biology tools. (wikipedia.org)
  • In 1989, he was a founding member of the Wellcome/CRC Institute for Cell Biology and Cancer (later Wellcome/CR UK) in Cambridge, and was its Chair until 2001. (wikipedia.org)
  • In 2004, the Wellcome Trust/Cancer Research UK Institute for Cell Biology and Cancer was renamed the Gurdon Institute in his honour. (wikipedia.org)
  • genetic
  • In genetic engineering, transposon tagging is a process where transposons (transposable elements) are amplified inside a biological cell by a tagging technique. (wikipedia.org)
  • The evidence from cloned animals shows that even apparently normal clones have subtle genetic abnormalities. (toledoblade.com)
  • The mother eventually bears offspring that is a genetic carbon copy of the adult who donated the cell. (toledoblade.com)
  • He described studies, done with scientists from the University of Hawaii, in which cloned mice were screened for genetic abnormalities. (toledoblade.com)
  • The clone is then transplanted into the nucleus of a cell from which genetic material has been removed. (thefreedictionary.com)
  • Linus Pauling and colleagues show in "Sickle Cell Anemia, a Molecular Disease" that a human genetic disease, sickle cell anemia, is caused by a molecular change in a specific protein, hemoglobin (1949). (wikipedia.org)
  • A nucleus is the organelle that holds a cell's genetic material (its DNA). (wikipedia.org)
  • Roslin's claimed clones are exact genetic copies of patent ineligible subject matter. (wikipedia.org)
  • particular phenotype
  • A gene responsible for a particular phenotype can be cloned within a given species, when movement is accompanied by the presence of a mutant phenotype. (wikipedia.org)
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. (wikipedia.org)
  • Genome
  • Genome browser BLAST Expression Search and Clone Search (search by gene symbol, gene name, or Affymetrix id) Gene nomenclature guidelines Literature search: Textpresso- uses an algorithm to match your search to specific criteria or section of a paper. (wikipedia.org)
  • Since the nucleus of virtually every animal cell contains the entire genome of the animal, it might seem easy enough to clone an animal by placing the nucleus in an egg cell from which the nucleus has been removed. (encyclopedia.com)
  • fertilisation
  • These new insights could boost efficacy of the alternative, non-oocyte-based iPSC techniques for stem cell banking, organ and tissue regeneration, as well as further our understanding of how cellular metabolism rejuvenates after egg-sperm fertilisation. (innovations-report.com)
  • frog
  • Anatomy and Development: images, fate maps, and videos Community Link --- jobs, labs which study Xenopus Protocol List- identify clones, antibodies, procedures Stock Centre- The National Xenopus Resource (maintains frog stocks, offers advanced research training) The Nobel Prize for Medicine or Physiology was awarded to John B. Gurdon and Shinya Yamanaka on October 8, 2012. (wikipedia.org)
  • Experiment: Gurdon used a technique known as nuclear transfer to replace the killed-off nucleus of a frog (Xenopus) egg with a nucleus from a mature cell (intestinal epithelial). (wikipedia.org)
  • tissues
  • The heart achieves its form through the specification, proliferation and organization of cardiac muscle cells and their supporting tissues. (biologists.org)
  • This nuclear body is distinct from the promyelocytic leukemia body (PML-NB), which is a subnuclear structure present in most cell types and tissues ( Salomoni and Pandolfi, 2002 ). (rupress.org)
  • In cloned pig study, discrepant expression pattern was identified in several cloned pig tissues, especially in brain, heart, and ear. (hindawi.com)
  • the gene is expressed in many tissues and exhibits an intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). (wikipedia.org)
  • 1839 - Theodor Schwann proposed that all animal tissues are composed of cells. (wikipedia.org)
  • Xenopus
  • The tadpoles resulting from these eggs did not survive long (past the gastrulation stage), however, further transformation of the nuclei from these Xenopus eggs to a second set of Xenopus eggs resulted in fully developed tadpoles. (wikipedia.org)
  • bacterial
  • Cloning into the bacterial virus bacteriophage λ allows use of fragments up to about 20 kb. (encyclopedia.com)
  • Bacterial artificial chromosomes (BACs) can contain up to 300 kb, and yeast artificial chromosomes (YACs), grown in yeast cells, can handle up to 2,000 kb, or 2 megabases. (encyclopedia.com)
  • sheep
  • In addition EFSA is to provide an update on the extent the current knowledge would apply to cloning of sheep, goats and chicken. (europa.eu)
  • EFSA is also seeking information in relation to the extent the current knowledge would apply to cloning of sheep, goats and chicken. (europa.eu)
  • clone a sheep. (thefreedictionary.com)
  • technique
  • While the Nobel Prize winner Dr Shinya Yamanaka had invented a technology called induced pluripotent stem cell (iPSC) reprogramming to replace the ethically controversial oocyte-based reprogramming technique, oocyte-based reprogramming was still deemed superior in complete cellular reprogramming efficiency. (innovations-report.com)
  • Several modifications of this technique allow cloning of even larger DNA fragments. (encyclopedia.com)
  • Mice cloned by the Honolulu technique were displayed at the Bishop Museum in Honolulu, Hawaiʻi, and the Museum of Science and Industry in Chicago, Illinois. (wikipedia.org)
  • They are the first cloned primates produced by this technique. (wikipedia.org)
  • Eggan's work as of 2007[update] has succeeded in developing a technique of merging stem and skin cells that has obtained considerable public attention as a possible avenue to avoid moral objections regarding stem cell research in the context of serious illness. (wikipedia.org)
  • It is a technique for cloning in which the nucleus of a somatic cell is transferred to the cytoplasm of an enucleated egg. (wikipedia.org)
  • In the United States, scientists at the Harvard Stem Cell Institute, the University of California San Francisco, the Oregon Health & Science University, Stemagen (La Jolla, CA) and possibly Advanced Cell Technology are currently researching a technique to use somatic cell nuclear transfer to produce embryonic stem cells. (wikipedia.org)
  • embryonic stem cel
  • In 2000, the NIH, under the administration of President Bill Clinton, issued "guidelines that allow federal funding of embryonic stem-cell research. (wikipedia.org)
  • In April 2004, 206 members of Congress, including many moderate Republicans, signed a letter urging President Bush to expand federal funding of embryonic stem cell research beyond what Bush had already supported. (wikipedia.org)
  • On July 29, 2005, Senate Majority Leader William H. Frist (R-TN), announced that he too favored loosening restrictions on federal funding of embryonic stem cell research. (wikipedia.org)
  • animal
  • this is the first instance of cloning of a vertebrate animal. (wikipedia.org)
  • The request is related to the recommendations included in the Opinion of the Scientific Committee of July 2008, in particularly those on the health and welfare aspects of animal clones. (europa.eu)
  • Safety of consumption of animal clones and their products (meat, milk products, eggs), including compositional and nutritional characteristics. (europa.eu)
  • The cells of an individual plant or animal are clones because they all descend from a single fertilized cell. (thefreedictionary.com)
  • So-called "testis" in an adult animal is a receptacle of cells from larval males. (wikipedia.org)
  • Animal cloning is about producing an animal that is essentially a copy of the original. (europa.eu)
  • EFSA's scientific advice will help inform risk managers in considering any future EU measures in relation to animal clones, their offspring and their products, such as meat and milk. (europa.eu)
  • EFSA adopted a scientific opinion on the implications of animal cloning on food safety, animal health and welfare and the environment in July 2008. (europa.eu)
  • EFSA received 128 submissions (285 including repetitive comments) during the public consultation on the implications of animal cloning on food safety, animal health and welfare and the environment, from 64 interested parties (including individuals, non-governmental organisations, industry organisations and national assessment bodies). (europa.eu)
  • In June 2009, EFSA published a statement that provided further scientific advice on animal cloning. (europa.eu)
  • Although Hwang had already established himself as an expert in animal cloning and secured celebrity status in South Korea in the late 90s, his alleged sudden success came as a surprise because this was the first reported success in human somatic cell cloning. (wikipedia.org)
  • The patent application claims the cloned animal. (wikipedia.org)
  • chromatin
  • 11. Chubb J.R., Boyle S., Perry P., Bickmore W.A. Chromatin motion is constrained by association with nuclear com-partments in human cell // Curr. (nsc.ru)
  • defects
  • The defects may explain why some cloned animals die younger, he said, noting that cloned mice have a life expectancy one-third shorter than naturally conceived mice. (toledoblade.com)
  • Eggan began to explore both this process and also the reasons that cloned animals often appeared to develop abnormally, with organ defects and immunological problems - his first contact with stem cell research. (wikipedia.org)
  • chloride
  • Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. (wikipedia.org)
  • the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. (wikipedia.org)
  • stem cell res
  • He was a professor of theriogenology and biotechnology at Seoul National University (dismissed on March 20, 2006) who became infamous for fabricating a series of experiments, which appeared in high-profile journals, in the field of stem cell research. (wikipedia.org)
  • On May 12, 2006, Hwang was charged with embezzlement and bioethics law violations after it emerged much of his stem cell research had been faked. (wikipedia.org)
  • The Korea Times reported on June 10, 2007, that Seoul National University fired him, and the South Korean government canceled his financial support and barred him from engaging in stem cell research. (wikipedia.org)
  • In February 2011, Hwang visited Libya as part of a $133 million project in the North African country to build a stem cell research center and transfer relevant technology. (wikipedia.org)
  • Federal funding for stem cell research had recently been removed, and part of his role was to obtain private funding to replace it. (wikipedia.org)
  • No federal law ever did ban stem cell research in the United States, but only placed restrictions on funding and use, under Congress's power to spend. (wikipedia.org)
  • On July 18, 2006, the Senate passed three different bills concerning stem cell research. (wikipedia.org)
  • progeny
  • There is no indication that differences exist in terms of food safety for meat and milk of clones and their progeny compared with those from conventionally bred animals. (europa.eu)
  • health and wel
  • Based on the request of the European Commission, EFSA investigated the health and welfare of clones during their life span and the causes of disease and mortality during the gestation period and early stages of life. (europa.eu)
  • 2001
  • In February 2001, George W. Bush requested a review of the NIH's guidelines, and after a policy discussion within his circle of supporters, implemented a policy in August of that year to limit the number of embryonic stem cell lines that could be used for research. (wikipedia.org)
  • artificial
  • She is the first "cloned" primate by artificial twinning, which is a much less complex procedure than the DNA transfer used for the creation of Zhong Zhong and Hua Hua. (wikipedia.org)