Cells amelogenin. Medical search. Frequent questions

Enamelysin cuts (cleaves) other proteins involved in enamel formation, such as amelogenin and ameloblastin, into smaller pieces. (medlineplus.gov)
Ameloblast differentiation in these cultures was investigated by analysis of the mRNA and/or protein expression of ameloblastin and amelogenin. (elsevierpure.com)
The enamel protein matrix consists of unique enamel proteins such as amelogenin, ameloblastin, and enamelin, which are secreted by highly specialized cells called ameloblasts. (tamu.edu)

Amelogenin, X isoform is a protein that in humans is encoded by the AMELX gene. (wikipedia.org)
The AMELX gene encodes for the structural modeling protein, amelogenin, which works with other amelogenesis-related proteins to direct the mineralisation of enamel. (wikipedia.org)
Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. (sigmaaldrich.com)
Autosomal recessive inheritance means that two copies of the MMP20 gene in each cell are altered. (medlineplus.gov)
The MMP20 gene mutations involved in this condition prevent cells from producing functional enamelysin. (medlineplus.gov)
We also demonstrated that the −657 to +411 DCT promoter fragment efficiently directs RB cell-specific transcription of the luciferase reporter gene in cell lines. (molvis.org)
The regulatory elements required for this cell-specific gene expression are likely located within its proximal promoter. (molvis.org)
A preliminarily study of carcinogenesis and chemosensitivity suggests that this cell model carries a tumor suppressor gene mutation and is sensitive to chemotherapy drugs. (oncotarget.com)
On a family-relationship DNA test report, this is called the amelogenin gene. (dnacenter.com)
In humans, the amelogenin gene is present on both the X and the Y chromosomes. (celljournal.org)
The aim of the present study was to evaluate the application of the amelogenin gene for assessment of chimerism in PBL and/or BM samples of patients who received sex-mismatched BMT. (celljournal.org)
PCR techniques using a set of amelogenin gene primer were preformed on whole WBC and/or BMA samples, and in some cases on isolated T-cell and granulocytes subsets of recipient patients. (celljournal.org)
The nonphosphorylated cytoplasmic DMP1 protein translocates to the cell nucleus and initially acts as a transcriptional factor to enhance gene transcription of the osteoblast-specific genes such as alkaline phosphatase and osteocalcin and then moves out to the extracellular matrix during the osteoblast to osteocyte transition phase to promote mineralization and phosphate homeostasis. (benthamscience.com)
Alternative splicing of the amelogenin gene in a caudate amphibian Plethodon cinereus. (rochester.edu)
Conclusion The downregulation of EMMPRIN expression can significantly decrease the invasion and migration of tumor cells, which lays a foundation for the further study of gene therapy of tumor with EMMPRIN. (shsmu.edu.cn)
Since the one cell cloning became the hurdle of making gene concentrating on duplicate, 217087-09-7 manufacture we attempted Slc2a3 to derive the transgenic cell lines from satellite television cells transfected with pEGFP-N1 plasmid as a model of transgenic satellite television cell. (researchensemble.com)
Assignment of the fibroblast growth factor 10 (FGF10) gene to bovine chromosome 20q16→q17 by fluorescence in situ hybridization and somatic cell panel analysis. (fbn-dummerstorf.de)

Without this protein's function, amelogenin and other proteins are not cleaved during enamel formation. (medlineplus.gov)
This Ebook will examine the multifunctional nature of a group of proteins known as the amelogenins. (benthambooks.com)
Derived from well-differentiated keratinizing squamous cell carcinoma of the buccal mucosa the TR146 cell line represents an in vitro model to study permeability, absorption and metabolism of various enzymatically labile drugs and substances including epithelial-mesenchymal transition (EMT) markers, tight junction proteins and aquaporins. (cancertools.org)
The cells secrete a variety of major plasma proteins e.g.. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. (hsanmartino.it)

Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation. (sigmaaldrich.com)
Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. (sigmaaldrich.com)
Our results suggest that interaction of epithelial and mesenchymal cells via the extracellular matrix is important for tooth differentiation in vitro. (elsevierpure.com)
It is, therefore, not clear whether haploinsufficiency for Xp genes in XO cells or the overdose of MSY and PAR genes in Xi(Y) cells is the cause of abnormal sex determination and differentiation in these animals. (veteriankey.com)

Adding MMP20 to amelogenin-chitosan hydrogel improves biomimetic enamel repair. (iadr.org)
Enamel matrix subunit compartment assembly and expansion coincide with C-terminal cleavage by the MMP20 enamel protease and N-terminal amelogenin self-assembly. (tamu.edu)

Using an evolutionary biology approach, we are now studying the relationship between the amelogenin molecule and enamel mechanical properties (Lucas et al. (rochester.edu)
At this stage of development, and upon further amelogenin cleavage, central and polyproline-rich fragments of the amelogenin molecule associate with the growing mineral crystals through a process termed "shedding", while hexagonal apatite crystals fuse in longitudinal direction. (tamu.edu)

Each and every human cell may contain 10,000 genes made up of DNA, and if you unwound and tied together all the DNA in that cell, it would stretch 6 feet . (dnacenter.com)
2009). To understand amelogenin molecular evolution, we cloned and characterized a number of reptilian and amphibian amelogenin genes, including those of frogs, salamanders, and iguanas (Wang et al. (rochester.edu)

In-depth characterization analysis of Penl1 cell line revealed tumorigenicity in immunodeficient mice, negative human papilloma virus (HPV) and mycoplasma infection, TP53 mutations and sensitivity to cisplatin and epirubicin. (oncotarget.com)

ß-galactosidase activity was specific for ameloblasts in incisors and molars, and islands of cells along developing molar roots. (ozgene.com)

In addition, amelogenins organize enamel rods during tooth development, and also aid in the development of cementum by directing cells that form the cementum to the root surface of the teeth. (benthambooks.com)
Amelogenin self-assembly into nanospheres has been recognized as a key factor in controlling the oriented and elongated growth of carbonated apatite crystals during tooth enamel formation. (techconnect.org)
Pseudogenized Amelogenin Reveals Early Tooth Loss in True Toads ( Anura: Bufonidae ). (rochester.edu)
[ 6 ] EMD is an extract of enamel matrix and contains amelogenins of various molecular weights, involved in the formation of enamel and periodontal formation during tooth development. (encyclopedia.pub)

Control the cell morphology and confluency under the microscope. (cytion.com)
MG-63, a cell that has fibroblast morphology isolated from the bone of a White, 14-year-old male patient with osteosarcoma. (atcc.org)

The expanded amelogenin polyproline region preferentially binds to apatite versus carbonate and promotes apatite crystal elongation. (rochester.edu)

Cells Tissues Organs. (wikipedia.org)
Expression of DCT was absent or barely detected in retina tissues, various human ocular cells, and major organs. (molvis.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
We successfully established two PeCa cell lines from fresh tumor tissues from 21 cases. (oncotarget.com)
METHODS: The expression levels of SMYD5 and the mitochondrial transcriptional coactivator peroxisome proliferator-activated receptor Î³ coactivator-1α (PGC-1α) were examined by Western blot, immunofluorescence staining, and immunohistochemistry in intestinal epithelial cells (IECs) and in colon tissues from human IBD patients and colitic mice. (bvsalud.org)
Also, satellite television cells offer a steady model for tissues system research, such as those regarding the transplantation of muscle-derived satellite television cells for muscles tissues renovation [2]. (researchensemble.com)

Virology: EBV-negative cell line re-infected with recombinant EBV whose genome is KO for EBER1 and EBER2 and which was knocked-in for EBER1 only. (cellosaurus.org)
Transwell assay was employed to determine the effect of recombinant interference plasmid on invasion and migration of SGC-7901 cells. (shsmu.edu.cn)
Transwell assay revealed that the number of cells with invasion and migration significantly decreased in SGC-7901 cells after transfection with recombinant interference plasmid. (shsmu.edu.cn)

The first human, trophoblastic endocrine cell type to be maintained in continuous culture. (sigmaaldrich.com)
Typically, human beings have 23 pairs of chromosomes in each cell. (dnacenter.com)
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran. (cellosaurus.org)
Antitumor effect of the novel sphingosine kinase 2 inhibitor ABC294640 is enhanced by inhibition of autophagy and by sorafenib in human cholangiocarcinoma cells. (cellosaurus.org)
Closely resembling normal human buccal epithelium, the cell line functions as a reliable oral mucosa model. (cancertools.org)
The cells will respond to stimulation with human growth hormone. (hsanmartino.it)
G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. (bcrj.org.br)
Objective To construct the short hairpin RNA (shRNA) of extracellular matrix metalloproteinase inducer (EMMPRIN), and investigate its influence on invasion and migration of human gastric cancer SGC-7901 cells. (shsmu.edu.cn)
It played a vital role in the development of the first polio vaccine and the discovery of telomerase in human cells. (the-dna-universe.com)
Fig 1: Difference between human primary cell culture and cell line (Oyeleye, O. et al . (the-dna-universe.com)
This has been observed with the human prostatic cancer cell lines JCA-1 and ALVA-31, as well as several thymic cell lines. (the-dna-universe.com)
The American Type Culture Collection (ATCC) and American National Standards Institute (ANSI) have documented (ASN-0002) the whys and hows of human cell line identification. (the-dna-universe.com)
STR profiling establishes a very unique fingerprint of every human cell line (or any other species). (the-dna-universe.com)
The ANSI/ATCC standard ASN-0002 has mentioned STR profiling as the preferred method for human cell line authentication. (the-dna-universe.com)

So Analysis of donor chimerism has become a routine method for documentation of engraftment after allogeneic stem cell transplantation. (celljournal.org)
We have investigated 30 allogeneic stem cell transplantation suffering from different types of leukemia (n=18) or non-malignant hematologic disorders (n=12) by close molecular monitoring during 1-12 months after transplantation. (celljournal.org)

This is based on amplification of highly repetitive yet stably inherited loci (short tandem repeats, STR) as well as the amelogenin locus for sex determination and separation of PCR products by capillary electrophoresis. (elsevierpure.com)

G uided bone regeneration (GBR) in the oral cavity is a procedure that attempts to restore bone tissue in a specific area, by the use of a cell occlusive membrane. (encyclopedia.pub)
The PEG membrane fulfils the main criteria for a resorbable GBR membrane having a cell-occlusive function, [ 2 ] slow reabsorption time, [ 3 ] and the ability to allow bone regeneration. (encyclopedia.pub)
RGD is a tripeptide (Arg-Gly-Asp) that by binding with integrins (transmembrane receptors that bind cells to the extracellular matrix) enhances bone regeneration. (encyclopedia.pub)

Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). (cytion.com)
Briefly rinse the cell layer with PBS without calcium and magnesium to remove all traces of serum which contains trypsin inhibitor. (bcrj.org.br)
Mapping amorphous calcium phosphate transformation into crystalline mineral from the cell to the bone in zebrafish fin rays. (mpg.de)

In elderly persons, squamous metaplasia of adenohypophyseal cells of the pituitary stalk or gland has been postulated as a possible origin for the papillary variant of craniopharyngioma. (medscape.com)
One cell line named Penl1 was isolated from a lymph node metastasis (LNM) of penile squamous cell carcinoma (PeSCC), usual type and comprehensively characterized here. (oncotarget.com)
Histology: well-differentiated keratinizing squamous cell carcinoma. (cancertools.org)
Impact of Ras association domain family 5 on cell migration and invasion of head and neck squamous cell carcinoma [J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2021, 41(6): 717-723. (shsmu.edu.cn)

No layer of initial enamel ribbons formed in the AmbnlacZ/lacZ mice, but pockets of amelogenin accumulated on the dentin surface along the ameloblast distal membrane and within the enamel organ epithelia (EOE). (ozgene.com)
Tumorigenic in nude mice, RT1112/84 cells possess a low plating efficiency requiring 400 cells/dish to form any colonies. (cancertools.org)

This cell line is a suitable transfection host. (bcrj.org.br)
After transfection, the expression of EMMPRIN mRNA and protein in SGC-7901 cells significantly decreased. (shsmu.edu.cn)

This plug acts as a lattice for the aggregation of platelets, the most common and "signature" cell type of the early inflammatory phase. (medscape.com)
Amelogenin nanoparticles in suspension: deviations from spherical shape and pH-dependent aggregation. (mpg.de)
The onset of amelogenin nanosphere aggregation studied by small-angle X-ray scattering and dynamic light scattering. (mpg.de)

In vitro cell culture model. (cytion.com)
Two types of collagen membrane-based in vitro culture systems were constructed in which dental epithelial and dental follicle cell lines were cultured. (elsevierpure.com)
However, for rapid and efficient screening of drug permeability, a cell line generally holds advantages over in vitro models of excised tissue. (cancertools.org)

Amelogenin cross-amplification in bovine species, goat and sheep and its application for sex determination. (fbn-dummerstorf.de)

Round and refractile cells in suspension. (sigmaaldrich.com)
Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). (cytion.com)
Add appropriate aliquots of the cell suspension to new culture vessels. (bcrj.org.br)

A conclusion We successfully cultured and isolated lamb principal satellite television cells via mechanical and enzymatic disaggregation. (researchensemble.com)

There is an increasing requirement for genetic analysis of individual cells from tissue sections. (elsevierpure.com)
This protocol describes a strategy to isolate cells using laser microdissection and to run a 17plex PCR to discriminate between cells of haplo-identical origin (i.e., fetal and maternal cells) in pancreas tissue but other robust DNA tests could be used. (elsevierpure.com)
Target cells are harvested from the tissue sections by laser microdissection and pressure catapulting (LMPC) prior to DNA profiling. (elsevierpure.com)
Figure 4.1: Histology and illustration of compact bone tissue, highlighting cells and layers of ECM. (pressbooks.pub)
In contrast, bone tissue is deposited in a circular layer upon circular layer, each cell working only in one layer. (pressbooks.pub)
Later, various other cell lines were developed from different tissue sources. (the-dna-universe.com)
Soft tissue regeneration and remodeling is fundamental in periodontal surgery, thus we investigated the angiogenic response elicited in the subcutaneous tissue of rats by a proprietary, polyethylene glycol hydrogel formulation (PEG) alone or conjugated with specific amelogenins (EMD) or nanobioglass particles (NBG). (encyclopedia.pub)

IL-1 stimulates the proliferation of inflammatory cells and promotes angiogenesis through endothelial cell replication. (medscape.com)

Effects of the bovine DGAT1 K232A and promoter VNTR loci on Somatic cell score and relative breeding value milk production in the German Holstein population. (fbn-dummerstorf.de)

Monitoring the engraftment of donor cell after allogeneic blood stem cell transplantation is important for the early diagnosis of graft failure or relapse of disease. (celljournal.org)

These cells-or more accurately, their cell bodies- are found within the pulp cavity, immediately adjacent to the dentin. (pressbooks.pub)

Cell culture was established from the left tumor which showed fetal rhabdomyomatous histology as described by Royer-Pokora 2010. (cytion.com)
Latest research have got demonstrated that fetal skeletal muscles satellite television cells possess a versatile potential to end up being utilized for transgenic pet creation by somatic cell nuclear transfer technique because these cells are muscle-derived control cells that can possibly expand and differentiate. (researchensemble.com)
To usage of fetal transgenic satellite television cells for nuclear transfer Prior, sex recognition of transgenic cell lines singled out from one cell cloning is certainly required because the gender of transgenic 217087-09-7 manufacture embryo can end up being motivated by sex recognition of nuclear donor cells. (researchensemble.com)

Department of Periodontics and Department of Cell Systems & Anatomy, School of Dentistry, University of Texas Health Science Center at San Antonio, San Antonio, Texas. (ozgene.com)

One co-culture method involved inoculation of one cell line into one side of the collagen membrane, and the other cell line into the opposite side of the membrane (sandwich co-culture). (elsevierpure.com)
As a control, the second method involved culture of one of the cell lines on a culture dish and the second cell line on a collagen membrane, facing away from the first cell line (separate co-culture). (elsevierpure.com)
The HAT- 7 cells were also grown as a monolayer culture on collagen. (elsevierpure.com)

Amelogenin is an extracellular matrix protein involved in the process of amelogenesis, the formation of enamel on teeth. (wikipedia.org)

Comparative histomorphology, genetic characteristics, and protein expression patterns revealed essential similarities between the cell line and its corresponding LNM. (oncotarget.com)
Name(s)=MYC-IGH (from parent cell line). (cellosaurus.org)
Zygosity=Heterozygous (from parent cell line). (cellosaurus.org)
This cell line is licensed to CLS by a university or institute. (cytion.com)
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). (cellosaurus.org)
Part of: JFCR39 cancer cell line panel. (cellosaurus.org)
The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (hsanmartino.it)
Cell line available for distribution. (hsanmartino.it)
5. Incubate the culture in a appropriate atmosphere and temperature (see "Culture Conditions" for this cell line). (bcrj.org.br)
Find out why you need to authenticate your cell line! (the-dna-universe.com)
In 1951, the widely known HeLa cell line was isolated from the cervical cancer biopsy of Henrietta Lacks. (the-dna-universe.com)
This immortalised cell line was the first one to grow exceptionally well in the laboratory. (the-dna-universe.com)
So are you sure that your cell line is what it is supposed to be? (the-dna-universe.com)
Cell line authentication - Needed for publishing, but how to do it? (the-dna-universe.com)
Many leading peer reviewed journals such as Nature, AACR Journals, Cell Biochemistry and Biophysics, etc. now require proof of cell line authentication before the submission of cell culture-based research articles. (the-dna-universe.com)
When to authenticate your cell line? (the-dna-universe.com)
In the past, techniques like isoenzyme analysis and karyotyping were used for cell line identification. (the-dna-universe.com)
STR profiling, however, is a reliable, low cost and easy method of cell line authentication. (the-dna-universe.com)
When a lab receives a cell line, relevant data regarding its origin, growth characteristics and culture conditions should be obtained (Reid et al . (the-dna-universe.com)
This cell line was deposited by A Billiau. (atcc.org)

As a result, we researched lifestyle and cell cloning of lamb satellite television cells to create a lamb cell series and to develop an principal satellite television cells sexing assay that was accurate, inexpensive and fast relatively. (researchensemble.com)

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). (bcrj.org.br)

To establish penile cancer (PeCa) cell lines for the study of molecular mechanisms of carcinogenesis and testing therapeutic reagents. (oncotarget.com)
STR DNA typing did not match any cell lines within three international cell banks. (oncotarget.com)
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
Use only Express Delivery (overnight) for cell lines. (addexbio.com)
Part of: COSMIC cell lines project. (cellosaurus.org)
Primary cholangiocellular carcinoma cell lines. (cellosaurus.org)
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A. An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines. (cellosaurus.org)
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling. (cellosaurus.org)
Part of: MD Anderson Cell Lines Project. (cellosaurus.org)
This is one of a number of cell lines derived from malignant gliomas. (bcrj.org.br)
Incubate cultures at 37°C. NOTE: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 6th edition, published by Alan R. Liss, N.Y., 2010. (bcrj.org.br)
Cell lines are essential for research and development! (the-dna-universe.com)
From the early 20th century, animal cell lines have been used for studies in medicine, cell biology, genetics, cancer research and drug development. (the-dna-universe.com)
Cell lines that were obtained from major cell culture collections or fellow researchers have since been used for the development of vaccines, drugs, reconstructive medicine and HIV treatment and testing. (the-dna-universe.com)
It was during the year 1968 when researchers first identified cross-contamination of cell lines. (the-dna-universe.com)
The researchers noted that overall characteristics and behaviour of their cell lines changed over time Eventually, they determined that contaminating agents such as adventitious microbes or other cell lines caused the changes. (the-dna-universe.com)
It has been estimated that a large proportion of the utilised cell lines are affected by the persistent problem of contamination or are even misidentified. (the-dna-universe.com)
2016). Additionally, reported studies carried out with misidentified or contaminated cell lines lead to questionable subsequent studies (Almeida et al . (the-dna-universe.com)
Here are the facts: About 80,000 cell lines have been developed and, 0.6% (488) of these was reported as misidentified. (the-dna-universe.com)
However, the estimate of misidentified cell lines in use is much higher. (the-dna-universe.com)
Researchers keep on using misidentified cell lines even after they are unmasked. (the-dna-universe.com)
Already in 1981, a report suggested that a large number of cell lines are contaminated with HeLa (more than 190 entries) followed by T-24 (20 entries) and HT-29 (18 entries) (Horbach and Halffman, 2017). (the-dna-universe.com)
Many protocols possess been set up for sexing the cell and embryos lines in farm pets. (researchensemble.com)

Amelogenin cross-amplification in the family Bovidae and its application for sex determination. (fbn-dummerstorf.de)

To download a certificate of origin for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
The certificate of origin for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)

11 Department of Molecular and Cell Biology, Verna and Marrs McLean Department of Biochemistry, Alkek Center for Molecular Discovery, Baylor College of Medicine, Houston, Texas. (aacrjournals.org)

A cell-based protein-protein interaction method using a permuted luciferase reporter. (leibniz-fli.de)

Expression of lnc-MTBP-5 in colorectal cancer and its effect on cell invasion [J]. JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY (MEDICAL SCIENCE), 2020, 40(09): 1193-1201. (shsmu.edu.cn)

Nanomaterials substrates are suitable for functioning as cell scaffolds. (techconnect.org)

Background This study was performed to explain the basic methods to isolate and culture of primary satellite cells (PSCs) obtained from 50 to 60-day-old sheep fetuses, single cell cloning of transfected sexing and PSCs of PSCs based on the ZFY/ZFX, amelogenin and high-motility-group (HMG) box sequences. (researchensemble.com)

IL-2 promotes further T cell proliferation to augment the immunogenic response to injury. (medscape.com)

Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. (cytion.com)
Resuspend the cells carefully. (cytion.com)
Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. (cytion.com)
If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. (cytion.com)
4.Discard the supernatant and Resuspend cell pellet with the recommended complete medium (see the specific batch information for the culture recommended dilution ratio). (bcrj.org.br)

Anatomy7

Organisms1

Diseases2

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

Technology, Industry, Agriculture2

Ameloblastin3

Gene17

Proteins4

Differentiation5

MMP202

Molecule2

Genes2

Mutations1

Ameloblasts1

Tooth4

Morphology2

Apatite1

Tissues6

Recombinant3

Human14

Allogeneic stem cell trans2

Locus1

Bone Regeneration3

Calcium3

Squamous4

Mice2

Transfection2

Aggregation3

Vitro3

Species1

Suspension3

Mechanical1

Tissue7

Endothelial cell1

Loci1

Stem cell transplant1

Dentin1

Fetal3

School of Dentis1

Collagen3

Formation1

Line20

Assay1

Trypsin1

Lines23

Determination1

Origin2

Biology1

Interaction1

Expression1

Suitable1

Methods1

Proliferation1

Resuspend5