Cells 3t3 cell line. Medical search. Frequent questions

3T3 cells are several cell lines of mouse embryonic fibroblasts. (wikipedia.org)
SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). (rndsystems.com)
Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts. (jci.org)
In contrast to renal epithelia, acid preincubation of NIH 3T3 fibroblasts led to suppression of Na/H antiporter activity. (jci.org)
RNA blots of 3T3 fibroblasts revealed the same size Na/H antiporter transcript as in MCT cells. (jci.org)
These studies demonstrate differential regulation of Na/H antiporter activity and mRNA abundance in renal epithelial cells and fibroblasts in response to an acidotic environment. (jci.org)
Below a geodesic path between two cells, an NIH 3T3 mouse fibroblasts cell and an A10 rat vascular smooth muscle cell is shown. (nist.gov)
Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity (XTT), membrane integrity (neutral red test), and total cell density (crystal violet dye exclusion test). (bvsalud.org)
This study shows that whole milk and HBSS are more efficient in maintaining the viability of 3T3 fibroblasts as demonstrated by three different cell viability tests. (bvsalud.org)
tooth avulsion, fibroblasts, cell viability. (bvsalud.org)
Cytocompatibility was assessed by a multiparametric assay employing 3T3 fibroblasts. (bvsalud.org)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Transforming and carcinogenic potential of cadmium chloride in BALB/c-3T3 cells. (cdc.gov)
Alterations of cancer-related genes in tumor cell lines derived from cadmium -transformed BALB/c-3T3 cells. (cdc.gov)

In vitro analysis with 3T3-F442A cells revealed anti-proliferative and anti-differentiation effects of OBEX. (nature.com)
In addition, its action on the 3T3-F442A cell line was tested in terms of regulation of proliferation capacity and differentiation into mature adipocytes as well as its effects on mature adipocytes. (nature.com)
In addition, on the 3T3-F442A cell line, OBEX inhibited proliferation of pre-adipocytes, blocked the differentiation of pre-adipocytes into new adipocytes, and decreased the lipid load in mature adipocytes. (nature.com)
A major limitation in approaching this problem has been the lack of suitable cell lines that exhibit normal, terminal, and synchronous differentiation in the absence of endogenous oncoproteins and in response to physiologic cytokines, and whose differentiation can be arrested by ectopically expressed human oncoproteins. (nih.gov)
Differentiation was normal and terminal as evidenced by morphology, cell surface markers, gene expression, and functional assays. (nih.gov)
The differentiation of the cells could be arrested by heterologous oncoproteins including AML1/ETO, PML/RARalpha, PLZF/RARalpha, Nup98/HoxA9, and other Hox proteins. (nih.gov)
These myeloid cell lines provide tools for examining the biochemical and genetic pathways that accompany normal differentiation as well as a system in which to dissect how other leukemic oncoproteins interfere with these pathways. (nih.gov)
The aim of this study was to investigate the effects of Ishige sinicola , a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. (degruyter.com)
A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. (degruyter.com)
Alteration by 2,3,7,8-Tetrachlorodibenzo-p-dioxin of CCAAT/enhancer binding protein correlates with suppression of adipocyte differentiation in 3T3-L1 cells. (aspetjournals.org)
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related compounds elicit multiple effects on the function of adipose tissue and adipogenic cell lines, including the suppression of adipocyte differentiation. (aspetjournals.org)
We began to examine the mechanism by which TCDD inhibits differentiation of the established preadipocyte cell line 3T3-L1. (aspetjournals.org)
Gel shift analysis of nuclear extract binding to an oligonucleotide containing a C/EBP DNA recognition sequence revealed no difference between extracts from control and TCDD-treated cells in the binding pattern at day 2 of differentiation. (aspetjournals.org)
These data indicate that suppression of differentiation in the 3T3-L1 preadipocyte cell line by TCDD occurs at a short but defined period, during the differentiation program, and involves altered regulation of C/EBP, including the inhibition of C/EBP alpha. (aspetjournals.org)
Analysis of transcription factor network underlying 3T3-L1 adipocyte differentiation. (tufts.edu)
The activation profiles of these TFs at different times following induction of adipogenic differentiation were quantified using 3T3-L1 reporter cell lines constructed to secrete the Gaussia luciferase enzyme upon binding of a TF to its DNA binding element. (tufts.edu)

Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
These sterile cell culture plates are great for the In-Cell Western Assay and a variety of other multiwell plate applications. (licor.com)
The simultaneous evaluation of different cell viability parameters, in a multiparametric assay, may identify more accurately any possible cytotoxic effects of storage media with immortalized cells intimately related to the in-vivo tissue. (bvsalud.org)

Increased Na/H antiporter activity has been demonstrated after in vivo chronic metabolic acidosis as well as in vitro acid preincubation of cultured rabbit renal tubule cells. (jci.org)

Here we identify CSNK1E , the gene encoding casein kinase 1 epsilon (CK1ε) as required specifically for the proliferation of breast cancer cells with activated β-catenin and confirm its role as a positive regulator of β-catenin-driven transcription. (plos.org)
The Wnt/β-catenin pathway plays a critical role in embryonic development, maintenance of multipotent progenitor cell populations and proliferation of many tissue types [1] , [2] . (plos.org)
We found that I. sinicola extract (ISE) increased cell proliferation in a dose-dependent manner. (degruyter.com)
To this end, the CP1 were fractioned by a series of chromatography procedures, and 51 peptides from the fraction possessing the most powerful cell proliferation ability were identified by LC-MS-MS. The peptides, GPAGPSGPAGK and GPPGSPGPR, were validated on a simultaneous basis as possessing enhanced bioactivity-inducing properties. (bvsalud.org)

Contact inhibited, a confluent monolayer of 3T3 cells yields approximately 40,000 cells per square centimeter. (fsu.edu)
Adherent cells grow as a monolayer. (wikipedia.org)
A confluent monolayer yields 40000 cells/cm2. (wikipedia.org)
RESEARCH particular, timing of return to roost, leaving for feeding, monolayer of Vero cells. (cdc.gov)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

IP: HeLa and NIH/3T3 whole cell lysates. (abcam.com)
Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. (rndsystems.com)
Simple Western™ analysis of lysates (0.1 mg/mL) from 3T3 cells using LC3A/B (D3U4C) XP ® Rabbit mAb #12741. (cellsignal.com)

Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank´s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. (bvsalud.org)

Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
Todaro and Green originally obtained their 3T3 cells from Swiss albino mouse embryo tissue. (wikipedia.org)

ICC/IF: HeLa cell line treated with TGF-beta. (abcam.com)
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg4A siRNA I #6427 (+), using Atg4A (D62C10) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). (cellsignal.com)
It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

In addition, OBEX induced a clear reduction of the lipid load in mature adipocytes obtained from 3T3-F442A cells. (nature.com)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)

Variants of the initial cell line have been tested and found negative for ectromelia virus (mousepox), but most are susceptible to polyoma and simian virus 40 ( SV40 ). (fsu.edu)
3T3 cells can be transformed with SV40 and some other polyomaviruses. (wikipedia.org)

The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. (wikipedia.org)

However, due to examination of 3T3 cells and subsequent research it has become widely accepted that for immortalization of cells to take place, telomere shortening, which can instigate chromosomal rearrangements, must be overcome, a process that is not necessarily related to a cell s ability to undergo oncogenic transformation. (fsu.edu)
We also find that expression of CK1ε is able to promote oncogenic transformation of human cells in a β-catenin-dependent manner. (plos.org)

One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

Photocytotoxicity is evaluated by the relative reduction in viability of cells exposed to the chemical in each of the two circumstances, which is measured by the degree to which they're able to absorb the neutral red dye. (peta.org)
When dental avulsion occurs, immediate replantation at the trauma site is the ideal procedure for maintaining the viability of periodontal ligament (PDL) cells. (bvsalud.org)
The viability of PDL cells relies on three critical factors: the duration of extra-alveolar time, preservation of the root and storage media of the tooth, all of which determine the prognosis for dental replantation 2-5 . (bvsalud.org)
Thus, to avoid further damage do the PDL cells, it is important to select an appropriate storage medium to keep their viability. (bvsalud.org)

MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines. (sigmaaldrich.com)

At the time of their establishment, 3T3 cells were different than most other cell lines in regard to the fact they did not induce tumors to develop when injected into murine species. (fsu.edu)
The cell lines were established by conditional immortalization of primary murine marrow progenitors with an estrogen-regulated E2a/Pbx1-estrogen receptor fusion protein. (nih.gov)

The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)
Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)
In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg4B siRNA I #6336 (+), using Atg4B (D1G2R) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.com)
Immunocytochemistry/ Immunofluorescence: FHL2 Antibody [NBP1-84978] - Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites. (novusbio.com)

In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. (sigmaaldrich.com)
Expression of the fusion protein cloned in pSNAP-CaaX can be achieved by transiently transfecting cells in culture with standard transfection protocols. (neb.com)
pSNAP-CaaX has performed well in stable and transient transfection of CHO-K1 and NIH 3T3 cells. (neb.com)
Twenty four to 48 hours after transfection begin selecting mammalian cultures in 600-1,200 µg/ml G418 (geneticin) depending on the cell line. (neb.com)
pCLIP-H2B performed well in stable and transient transfection of CHO-K1, COS-7, U-2 OS and NIH 3T3 cells. (neb.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)

Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3×105 cells per 20 cm2 dish (the second "3") continuously. (wikipedia.org)

At a time when the idea that cancer had a genetic basis was widely disbelieved, Nowell's results provided the first clear evidence that a particular genetic defect in a single chromosome can lead to a population or clone of identical cells that accumulate in numbers to form a deadly malignancy. (laskerfoundation.org)

Decreased apoptosis in tumor cells and transformed cells induced by cadmium chloride and beryllium sulphate. (cdc.gov)
Nowell collaborated with the late David Hungerford who, he says, "knew more about chromosomes than I did," and together they made the startling observation that individuals suffering from chronic myelogenous leukemia (CML) had an abnormally small chromosome in the tumor cells. (laskerfoundation.org)

The peptide PATGDLTDFLK showed excellent DPPH scavenging activity (39%) and a good cytoprotective effect on H2O2-induced oxidative stress damage in HepG2 cells with a 21.1% increase observed. (bvsalud.org)

Figure 1: Comparison of analyzers with respect to their ease of implementation in microbial fermentations and mammalian cell culture unit operations for various quality attributes: (a) misincorporation, (b) nutrients, (c) glycosylation, and (d) cell growth. (biopharminternational.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)

In Europe, the 3T3 neutral red uptake (NRU) phototoxicity test was developed and validated to show its applicability and relevance to humans and has since been accepted at the international level as a total replacement for animal-based phototoxicity studies. (peta.org)

We used three nonparametric tests, the Friedmann-Rafsky Minimum Spanning Tree test, the Schilling Nearest Neighbor test and an energy test to test the hypothesis that two cell populations are statistically equivalent. (nist.gov)

It is possible to use pools of stable cell populations for initial cell labeling to test for the presence of SNAP-tag expression. (neb.com)

Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
The certain peptide was capable of regulating the myogenic cell cycle and inhibiting myogenic cell apoptosis. (bvsalud.org)

Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)

One day while "diddling around with leukemic cells in culture," and rinsing them with tap water, Nowell noted that cells were dividing. (laskerfoundation.org)
I came to realize that there were many questions about chromosome changes in patients that would be rewarding to study," noted Rowley, and for the next decade she labored over the microscope looking at chromosomes in leukemic cells. (laskerfoundation.org)

Visualizing Structural Features of the Golgi Complex and Nucleus in Swiss Mouse Embryo Cells - In order to examine structural features of the Golgi complex and nucleus at relatively high magnification, a log-phase culture of 3T3 cells was fixed, permeabilized, blocked with normal goat serum, and then treated with rabbit anti-giantin (Golgi protein) primary antibodies followed by goat anti-rabbit secondary antibodies (IgG) conjugated to Alexa Fluor 568. (fsu.edu)
Later, as a principal investigator position at the National Cancer Institute in Bethesda, Maryland, Todaro repeated the isolation procedure from the NIH Swiss mouse embryo with his students and established NIH-3T3 cell line. (wikipedia.org)
3T3 mouse cells are hypertriploid. (wikipedia.org)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
Na/H antiporter activity was increased by 60% in a mouse renal cortical tubule cell line (MCT), and by 90% in an opossum kidney cell line (OKP) after 24 h of preincubation in acid (low [HCO3]) media. (jci.org)
Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. (novusbio.com)

Note that the intensity of the fluorescence may vary depending on cell line and labeling substrate used. (neb.com)
Labeling of transfected cells with a fluorescent SNAP-Cell substrate should show strong inner cell membrane fluorescence. (neb.com)
Labeling of transfected cells with a fluorescent CLIP-Cell substrate should show strong nuclear fluorescence. (neb.com)
This observation suggests that the outcome of a probing action and not substrate properties alone can explain the guidance behavior of 3T3 cells. (northwestern.edu)

Macrophages are important immune cells that participate in both innate and adaptive immune responses, such as phagocytosis, recognition of molecular patterns, and activation of the immune response. (frontiersin.org)
Macrophages are immune cells widely distributed throughout the body and are components of innate immunity. (frontiersin.org)
Glycans are one of the four fundamental macromolecular components of all cells and are highly regulated in immune cells, including macrophages. (frontiersin.org)

Understanding cellular responses to stimuli, such as pharmaceuticals or environmental toxins and discovering correlations between cellular responses and the cell phenotype are applications motivating this work. (nist.gov)
Also, using image analysis and visualization software for tracking live cells developed at by the Cell Systems Science Group, we have begun using our results to track, over time, the relationship between cellular characteristics (expressed by GFP intensity) and cell shapes changes. (nist.gov)
Regarding the phenomenon of cellular guidance itself, 3T3 cells faced with a choice between guiding lines toward different directions seem to explore various options before following one. (northwestern.edu)

previous to studies of the 3T3 line, it was believed that these characteristics were necessarily synonymous with one another. (fsu.edu)

Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Immunoblot analysis with specific antibodies to each C/EBP isoform demonstrated that the levels of C/EBP delta and C/EBP beta protein were rapidly induced (by day 1) and then abrogated by day 4 and 8, respectively, in solvent-treated (control) cells. (aspetjournals.org)
A critical evaluation of suitability of each analytical method as a PAT tool in terms of sampling (in-line, at-line, or on-line), sample preparation, duration of analysis, and its industrial application is performed. (biopharminternational.com)
Western Blot: FHL2 Antibody [NBP1-84978] - Analysis in human cell line U-2 OS. (novusbio.com)

Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)

This statistical application involves determining the similarity of cell populations based on comparing cell shapes. (nist.gov)
The process starts with fluorescence microscope images of cell populations. (nist.gov)
For two populations of cells, dissimilarity matrices are created whose entries are geodesic distances between cell shape pairs. (nist.gov)
Often cell populations contain hundreds of cells and a dissimilarity matrix may have ten thousand or more entries. (nist.gov)

The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3×105 cells. (wikipedia.org)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)

Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)

Flow Cyt (intra): C2C12 cells. (abcam.com)

This report describes clonal, granulocyte-macrophage colony-stimulating factor-dependent myeloid cell lines that exhibit these properties. (nih.gov)
The tiny Philadelphia chromosome became a clear and consistent marker of CML, a cancer of the myeloid or bone marrow cells, with broad implications for diagnosis and prognosis of disease. (laskerfoundation.org)

The modal chromosome number is 68, which occurs in 30% of cells. (wikipedia.org)

In addition clonal cell lines can be isolated and characterized if desired. (neb.com)

The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3' cells. (wikipedia.org)
Freshney's Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. (wikipedia.org)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
His current research is on wound healing, anti-cancer, anti-obesity and anti-diabetes study of several medicinal plants using animal cell culture. (iium.edu.my)

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