• The expression of c‑Myc and cyclin D1 downstream of the Wnt signaling pathway were detected using reverse transcription‑quantitative polymerase chain reaction analysis. (spandidos-publications.com)
  • Because the consensus motif for the TCF-4 binding site (A/TA/TCAAAG) is present in the promoter region (6 , 7 , 8 , 9 , 10) , c-myc, cyclin D1, c-jun, and MMP7 are considered targets for the Wnt signaling pathway. (aacrjournals.org)
  • In addition, NKP608 reduced expressions of Wnt-3a, β-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. (conicyt.cl)
  • In developing white matter, decreased total β-catenin, activated β-catenin, and cyclin D1 levels coincided with the peak of Sox17 expression, and immunoprecipitates showed a developmentally regulated interaction among Sox17, T-cell transcription factor 4, and β-catenin proteins. (jneurosci.org)
  • These results indicate that Sox17 suppresses cyclin D1 expression and cell proliferation by directly antagonizing β-catenin, whose activity in OPCs is stimulated not only by Wnt3a, but also by PDGF. (jneurosci.org)
  • Wnt (Wingless and integration site) factors are secreted glycoproteins Which direct cell proliferation, polarity, and fate decisions during embryonic development. (jneurosci.org)
  • This special issue is composed of six reviews and two original articles selected to highlight recent advances in the role of Wnt signaling in CNS embryonic development, in adult brain function, in neurodegenerative conditions such as Alzheimer's disease, schizophrenia, NeuroAIDS, and in gliomas. (springer.com)
  • Consistent with this, ARMC8α downregulation by siRNA knockdown inhibited growth, colony formation, and invasion in A549 lung cancer cells, while ARMC8α overexpression promoted growth, colony formation, and invasion in H1299 lung cancer cells. (springer.com)
  • In this study, the expression level of GSK-3β was measured in 4 human HCC cell lines, and the small interfering RNA (siRNA) vectors against or plasmids encoding GSK-3β were used to evaluate the responses of target cells to the knockdown or overexpression of this kinase, respectively. (jcancer.org)
  • The knockdown of GSK-3β by siRNA led to a decreased expression of the epithelial junction molecules (ZO-1, E-cadherin) and an increase in the expression of a mesenchymal cell marker (α-SMA) and a gene transcription factor (β-catenin), resulting in enhanced tumor cell dissemination. (jcancer.org)
  • In cells that usually do not get a Wnt transmission, cytoplasmic -catenin will a multi-protein -catenin damage complicated that contains many proteins including Axin, APC, and glycogen synthase kinase-3 (GSK3), which is constitutively phosphorylated at a cluster of Ser and Thr residues at its N-terminus by GSK3. (cancercurehere.com)
  • GSK3ß (glycogen synthase kinase 3ß), is a key mediator in the Wnt pathway and is involved in regulating ß catenin cytoplasmic and nuclear distribution. (ed.ac.uk)
  • In the absence of a Wnt signal, GSK3p, as a part of an axin and APC (adenomatous polyposis coli) complex, phosphorylates ß catenin and induces its degradation via the ubiquitin/ proteosome pathway. (ed.ac.uk)
  • Significantly, Dorsky and his colleagues also found that mice displayed a similar pattern of Wnt activity. (healthcanal.com)
  • Western blot revealed that transfection with CTHRC1 could significantly promote CTHRC1 expression in prostate cancer cells. (alliedacademies.org)
  • Clone formation assay revealed that transfection with CTHRC1 could significantly promote the proliferation of prostate cancer cells. (alliedacademies.org)
  • Wound healing assay and Transwell test revealed that transfection with CTHRC1 could significantly promote the migration of prostate cancer cells. (alliedacademies.org)
  • In this study, we found that lncRNA MEG3 was significantly downregulated in malignant glioma tissues and cell lines. (ovid.com)
  • Our results showed that GSK-3β expression was significantly lower in human HCC cell lines with high metastatic potential than that in HCC cell lines without metastatic characteristics or in a normal human liver cell line. (jcancer.org)
  • Sulindac significantly inhibited proliferation of HT-29 colon cancer cells in a dose- and time-dependent manner. (tumorijournal.com)
  • Higher concentrations of Axin and lower concentrations of APC are present in mammalian cells. (nih.gov)
  • Axin concentrations are greater than APC in kidney epithelial cells, whereas in intestinal epithelial cells the APC concentration is higher than Axin. (nih.gov)
  • E-cadherin, APC, R-spondin and Axin mutations have also been documented in tumor samples, underscoring the deregulation of this pathway in cancer. (cellsignal.com)
  • Taken together, our results demonstrated that NKP608 inhibited colorectal cancer cell proliferation, migration and invasion via suppressing the Wnt/β-catenin signaling pathway. (conicyt.cl)
  • The DLD1 cancer of the colon cells express crazy type -catenin at abnormally high amounts due to lack of APC. (cancercurehere.com)
  • Amazingly, conditional manifestation of TrCP-E-cadherin beneath the control of a tetracycline-repressive promoter in DLD1 cells selectively knocked down the cytosolic, however, not membrane-associated subpopulation of -catenin. (cancercurehere.com)
  • Our outcomes suggest that a higher focus of cytoplasmic -catenin is crucial for the development of colorectal tumor cells. (cancercurehere.com)
  • History Wnt signaling performs diverse functions at many phases of advancement by regulating the balance of -catenin . (cancercurehere.com)
  • Membrane-associated -catenin takes on an important part in cell-cell adhesion. (cancercurehere.com)
  • Meanwhile, over-expression of miR1-2 in mouse BMSCs enhanced the expression of wnt11, JNK, β-catenin and TCF in the Wnt/β-catenin signaling pathway. (biomedcentral.com)
  • The availability of ß-catenin at the cell membrane, in the cytoplasm and in the nucleus plays a key part in the process of mesenchymal condensation during chondrogenesis, which is regulated by Wnt signalling and interaction with other signalling pathways. (ed.ac.uk)
  • Using RT-PCR, cloning, immunofluorescence and confocal microscopy it was for the first time demonstrated that the Wnt signalling components,Wnt-l, Fz-2, Fzrp and ß catenin were expressed in human chondrocyte cell lines. (ed.ac.uk)
  • Furthermore, our findings showed that highly expressed MEG3 could weaken Wnt/β-catenin signaling in glioma. (ovid.com)
  • Collectively, our findings revealed that downregulated lncRNA MEG3 could promote glioma cell proliferation through targeting Wnt/β-catenin signaling, which mainly influenced cell cycle. (ovid.com)
  • In conclusion, GSK-3β might play a vital role in suppressing HCC dissociation by preventing the disassembly of cancer cell epithelial junctional complex via the GSK-3β/β-catenin pathway. (jcancer.org)
  • Whether the Wnt/β-catenin pathway is activated or not depends on the stability of β-catenin in the cytoplasm. (biomedcentral.com)
  • In the absence of Wnt stimulation, β-catenin is phosphorylated by the complex and degraded by the ubiquitination/proteasome pathway. (biomedcentral.com)
  • Finally, inhibiting Wnt/β‐catenin signaling pathway attenuated the effect of exogenous MALAT1. (ndsl.kr)
  • The focus of this special issue is on Wnt/β-catenin and its diverse physiological cell signaling pathways in neurodegenerative and neuropsychiatric disorders. (springer.com)
  • Al-Harthi L (2012) Interplay between Wnt/β-catenin signaling and HIV: Virologic and biologic consequences in the CNS. (springer.com)
  • After the embryo is formed, some neural stem cells lie dormant in the brain and spinal cord until they are activated to serve as a repair system. (healthcanal.com)
  • The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. (conicyt.cl)
  • Over-expression of CTHRC1 can increase the invasion ability of colorectal cancer cells [ 15 ]. (alliedacademies.org)
  • These parameters provide the basis for formulating Wnt signalling models for kidney/intestinal epithelial mammalian cells. (nih.gov)
  • CTHRC1 can promote tissue repair and cell migration by limiting deposition of collagen matrix [ 7 ]. (alliedacademies.org)
  • Association of Notch ligands with Notch receptors on neighboring cells leads to cleavage of Notch receptors by metalloprotease and γ-secretase to induce nuclear translocation of Notch intracellular domain (NICD). (spandidos-publications.com)
  • However, the induction ofGSK3ß activity following mechanical stimulation was mediated by a PI3K dependent pathway rather than a Wnt pathway. (ed.ac.uk)
  • In our earlier work, we discovered that Wnt signaling was required for neurogenesis in the embryonic zebrafish hypothalamus," says Richard Dorsky, Ph.D., associate professor of neurobiology and anatomy at the University of Utah School of Medicine and senior author on the study. (healthcanal.com)
  • We also found that, in zebrafish, both Wnt signaling and hypothalamic neurogenesis continue into adulthood. (healthcanal.com)
  • From a functional standpoint, it is not yet clear why the ability to continuously produce hypothalamic nerve cells is important in adult zebrafish," says Dorsky. (healthcanal.com)