Cell viability in hela cells. Medical search. Frequent questions

Here we show that methanolic extracts of Sapota fruit (MESF) induces cytotoxicity in a dose-dependent manner in cancer cell lines. (nature.com)
In a preliminary study it has been shown that phenolic antioxidants such as methyl 4- O -galloylchlorogenate and 4- O -galloylchlorogenic acid derived from Sapota fruits can induce cytotoxicity in colon cancer cells 23 . (nature.com)
37.7 g/ml over the viability of HeLa cells using cytotoxicity MTT assay. (columbiagypsy.net)
The components were tested just at two concentrations, 20 and 200 g/ml, for his or her inhibitory influence on the monophenolase and diphenolase triggered types of tyrosinase (F.E) exhibited the best cytotoxicity aftereffect of Hela cells set alongside the additional components. (columbiagypsy.net)
Moreover, no cytotoxicity towards mammalian and red blood cells at the concentrations tested was observed for any of the compounds under investigation. (ibecbarcelona.eu)
The results also indicated that the environment (i.e. phosphate buffer or culture medium) used to disperse the nanomaterials prior to performing the viability tests can have a significant impact on their cytotoxicity. (infim.ro)
Confluent monolayers of cells were used to test the cytotoxicity ethanolic extracts. (jptcp.com)
After adding vitamin C reagent to plated HeLa cells, we performed cytotoxicity assays to measure cell viability. (csp.edu)
To evaluate the cytotoxicity of these substances, we treated uninfected cells for 48 h with the maximum doses as described. (cdc.gov)

Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
Assay advancement and marketing for HTS Assay advancement and optimization had been conducted in normal or specialized (fluorescence or luminescence) 96-well flat-bottom plates into which 100 μL of cells was seeded per well. (exposed-skin-care.net)
Endometrium-derived epithelial cells (Ishikawa) showed no response to the natural compound by using a cell viability assay (MTT). (nutrimedical.com)
In vitro antiproliferative potential was determined by MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] on HeLa cell lines. (jptcp.com)
The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. (ox.ac.uk)
Cell viability was determined with Trypan Blue or MTT tests, or by a DNA-fragmentation (comet) assay. (nwu.ac.za)
Before freezing and 24 h after thawing, cell viability was assessed using live-dead assay. (huji.ac.il)
The effect of compounds 1 and 2 on cell viability of HeLa and HepG2 cells was investigated with the MTT assay. (unibas.ch)
Cell membrane integrity and cell viability were evaluated by measuring trypan blue exclusion assay and reduction of the tetrazolium-blue compound, respectively. (ac.ir)
We treated Huh7 cells with recombinant human IFN-α2a (PBL Assay Science, https://www.pblassaysci.com ) or recombinant human IFN-γ (R&D Systems, https://www.rndsystems.com ) 16 h before and 2 h after infection. (cdc.gov)

In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines. (biomedcentral.com)
In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines. (biomedcentral.com)
Therefore, the aim of this study was to evaluate the cytotoxic and anti-proliferative properties of Cannabis sativa and its isolate, cannabidiol in cervical cancer cell lines. (biomedcentral.com)

It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Previous findings show that six of these toxins have the ability to kill cancer cells: Parasporin-1 (HeLa), Parasporin-2 (HepG2 and MOLT-4), Parasporin-3 (HL60 and HepG2), Parasporin-4 (Caco-2, Sawano, and MOLT-4), Parasporin-5 (no specificity), and Parasporin-6 (HepG2, HeLa, and normal). (tnstate.edu)

The 0.01) inhibition on cell viability/proliferation on the concentrations which were used. (columbiagypsy.net)
Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured. (nutrimedical.com)
Ellagic acid significantly reduced HOS cell proliferation, and induced apoptosis evidenced by chromosomal DNA degradation and apoptotic body appearance. (nutrimedical.com)
The Effect of Vitamin C on HeLa Cell Proliferation" by James D. Dinger, Allysa C. Steinhaus et al. (csp.edu)
Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. (biomedcentral.com)
These compounds are almost structurally similar or possess identical pharmacological activities and offer various potential applications including the ability to inhibit cell growth, proliferation and inflammation [ 22 ]. (biomedcentral.com)
These results indicated that A3G did not significantly affect the cell line proliferation, but a comparison of IC50 and CC50 of aviprin revealed that, in the concentrations of CC50 aviprin, at least 42.7% of LNCaP cells were not dead by necrosis. (ac.ir)

Overall although some studies reported several flavones to possess capability to inhibit PARP-1 in individual pulmonary epithelial and vascular endothelial cells (Geraets et al . (exposed-skin-care.net)
In the current study, we exposed cultured human epithelial (HeLa) cells acutely and repeatedly to ozone and investigated the effects thereof on cell viability. (nwu.ac.za)
We cultivated human lung epithelial A549 cells (ATCC CCL-185), human hepatoma Huh7 cells ( 13 ), human cervix carcinoma HeLa cells (ATCC CCL-2), African green monkey kidney Vero cells (ATCC CCL-81), and transformed human dermal fibroblast cultures ( 14 ) in Dulbecco modified eagle medium supplemented with 10% fetal calf serum at 37°C and 5% CO 2 . (cdc.gov)

The survival rates of tumor patients are decreasing because of tumor cell resistance to anticancer chemotherapies [ 1 ]. (oncotarget.com)
This is due to acute alterations in the tissues surrounding the tumor and in the environment as a result of treatment with anticancer therapeutics, which facilitate the drug resistance of tumor cells. (oncotarget.com)
Comparative anticancer activities of Ficus carica and Ficus salicifolia latex in MDA-MB-231 cells. (jptcp.com)
We also report the mechanism of action of compound 2 as a potential anticancer agent in HeLa cells. (unibas.ch)

In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell. (biomedcentral.com)
Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
Flower and bulb extracts exhibited dose-dependent antiproliferative effects on non-transformed and transformed cells, with greater growth inhibition in A549 lung cancer cells at lower concentrations. (ijpsonline.com)
40%) inhibition from any of the delta-endotoxins on HeLa cells. (tnstate.edu)
It was found that the delta-endotoxins in high concentrations 75µg/µl and 87.5µg/µl from Bt 5 and Bt 6 respectively, show 96% and 82% inhibition of growth against MCF-7 cells. (tnstate.edu)
However, further studies must investigate purified delta-endotoxins and their mechanism of action on various cell lines and determine the minimum concentration for growth inhibition. (tnstate.edu)
Cannabis sativa in particular cannabidiol, we propose it plays important role in helping the body fight cancer through inhibition of pain and cell growth. (biomedcentral.com)
These data point to the greater sensitivity of HeLa cell line than the LNCaP cell line, and therefore, their inhibition by necrosis could be anticipated. (ac.ir)
The study of DNA fragmentation provided further evidence for the efficient inhibition of the LNCaP cell line via apoptosis induction. (ac.ir)

The cytotoxic activities of the extracts were determined using cell viability, deoxyribonucleic acid fragmentation and caspase-3 activity tests. (ijpsonline.com)
cSCK led to greater silencing efficiency than Lipofectamine 2000 in HeLa cells as determined by the viability following transfection with cytotoxic and non-cytotoxic siRNAs, as well in 293T and HEK cells, and was comparable in BEAS-2B and MCF10a cells. (wustl.edu)

Cell cycle analysis suggested activation of apoptosis, without arresting cell cycle progression. (nature.com)
Annexin V-propidium iodide double-staining demonstrated that Sapota fruit extracts potentiate apoptosis rather than necrosis in cancer cells. (nature.com)
Since cells with uncompromised membranes may retain esterase activity and an early stage of apoptosis is cessation of metabolic activity, electrochemical metabolic activity measurements may be an early indicator of programmed cell death. (nist.gov)
Anti-CD95-stimulated cells show an increase in Annexin V-positive and BD Via-Probe™ Green-positive cells, indicating an increase in apoptosis and cell death. (bdbiosciences.com)
All three extracts induced apoptosis in HeLa cells. (biomedcentral.com)
Apoptosis an active physiological process resulting in cellular self-destruction of unwanted cells is absent in cancer cells. (biomedcentral.com)
CONCLUSION: Ellagic acid exhibited both antioxidant activity in V79-4 cells and apoptosis-inducing activity in HOS cells through the up-regulation of Bax and activation of caspase-3. (nutrimedical.com)
Chemical characterization of non‐psychoactive Cannabis sativa L. extracts, in vitro antiproliferative activity and induction of apoptosis in chronic myelogenous leukaemia cancer cells. (jptcp.com)
Taken together, these results suggest that treatment of HeLa cells with compound 2 can induce apoptosis by regulating Bcl-2 family members and by suppressing caspase cascade activation. (unibas.ch)

Dark field microscopy and CV-OCT are used to evaluate the specific adhesion of these functionalized GNSs to activated endothelial cells. (bvsalud.org)

We reckon that the data of activity for 4MF and DMF on the micromolar concentrations analyzed within this research is in keeping with reviews of several prior elegant studies executed in neuronal and non-neuronal cells or tissue for instance (Schroeter et al . (exposed-skin-care.net)
2012 with flavonoid activity against PARP in cell lifestyle versions having been more often than not reported at micromolar concentrations (Geraets et al . (exposed-skin-care.net)
Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
Finally, lipid accumulation in cells induced to undergo adipogenesis and treated with BPC decreased although without significant differences at different concentrations. (unav.edu)
0.05) in the cell viability for all groups treated with PDT, in all concentrations tested. (univap.br)
In the internalization cell study was possible to observe the internalization of phthalocyanine after one hour of incubation, at 37 ◦C, with the the accumulation of PS in the cytoplasm and inside the nucleus at both concentrations tested. (univap.br)
Low concentrations of 123I-labeled antisense PNA-YR9*ODN complexed with cSCK, showed significantly higher retention of radioactivity in iNOS-induced RAW 264.7 cells when compared to a mismatched PNA. (wustl.edu)
In this study, we investigated the impact of vitamin C on cervical cancer cells at varying concentrations. (csp.edu)
We hypothesized that higher concentrations of vitamin C would lead to lower cell viability, as vitamin C is predicted to cause cell death in cancer cells. (csp.edu)
Our results indicated that higher concentrations of vitamin C do not necessarily correlate with lower cell viability of cervical cancer cells. (csp.edu)

The purpose of this study was to investigate the antiproliferative effect of the methanol and ethyl acetate extracts of Ornithogalum balansae flower and bulb against normal ARPE-19 cell line and cancer cell lines, A549, CRL-2923, HT-29 and HeLa. (ijpsonline.com)
The IC50 values of the flower extracts against A549 cells were 1.5±0.153 and 2.2±0.503 µg/ml, and of the bulb extracts were 1.8±0.220 and 1.56±0.106 µg/ml, respectively. (ijpsonline.com)
Finding caspase-3 activity of the methanol flower extract in A549 indicated apoptotic effects in those cells. (ijpsonline.com)
These results showed that Ornithogalum balansae extracts could exhibit antitumor activity particularly on the A549 lung cancer cell line, a resistant cell line. (ijpsonline.com)
Further investigations of the composition of the extracts are required to identify the effective components with antiproliferative activity, particularly on A549, the human alveolar adenocarcinoma cancer cell line. (ijpsonline.com)
This study revealed that extracts of Ornithogalum balansae possessed considerable antiproliferative activity against A549 cells. (ijpsonline.com)

Results showed that with the exposure concentration increase, ROS and CCP in Hela cells presented a significant increase but GSH contents in Hela cells and cell viability showed a significant decrease respectively compared with the control. (scirp.org)
4C5] The sample extracts were dissolved in 10% dimethyl sulfoxide (DMSO) inside a sterile Middlebrook 7H9 broth bottom, to secure a stock options concentration FTY720 cell signaling of 50.0 mg/ml. (columbiagypsy.net)
Dilute ER Flipper-TR® DMSO stock solution to the desired concentration (start with 1 µM) in cell culture medium shortly before applying to the cells (Apply quickly, max 5 minutes, the staining solution to the cells of which the growth medium was removed). (cytoskeleton.com)
A dose dependent curve was obtained by plotting concentration of dose and cell viability. (jptcp.com)
Treating the LNCaP cell line with various concentration of the furanocoumarins showed the IC50 of aviprin and A3G 0.4 and 6.6 mg/ml, whereas their CC50 were 0.7 and 11 mg/ml, respectively. (ac.ir)

Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
Spectacular increase in transfection efficiency and dramatic decrease in cell toxicity. (thermofisher.com)
In addition, TSA-mediated cell death was reduced by the overexpression of HIF-1α but it was rescued by transfection with a HIF-1α mutant (K674R). (oncotarget.com)
We detail reverse transfection of cells with siRNAs, followed by ionizing radiation, fixing, and staining of the plates for automated colony counting. (ox.ac.uk)
Herein, cSCK showed good siRNA binding and facilitated efficient siRNA transfection in HeLa, a mouse macrophage cell line and other human cell lines. (wustl.edu)
cSCK also led to greater silencing of iNOS expression than Lipofectamine 2000 in a mouse macrophage cell line, and provided greater protection from serum degradation, demonstrating its potential usefulness as an siRNA transfection agent. (wustl.edu)

Thus, the toxins from these strains do not belong to the parasporins groups that inhibit HeLa cells. (tnstate.edu)

Methanolic, n-Hexane and chloroform extracts of Nigella sativa seedz effectively killed HeLa cells. (biomedcentral.com)
Ethanolic extracts were used in 3T3-L1 cell culture. (unav.edu)
Against ARPE-19 cell line the IC50 values were 22.5±6.466 and 25±2.887 µg/ml for flower and 14±4.726 and 12.5±3.182 µg/ml for bulb extracts, respectively. (ijpsonline.com)
Antisense binding sites on the iNOS mRNA were first mapped by a procedure for rapidly generating a library of antisense accessible sites on native mRNAs (MASL) which involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific PCR. (wustl.edu)

This compound efficiently entered cells through organic anion-transporting polypeptides to accumulate in the cellular mitochondria. (worldmedicinefoundation.com)
HeLa cells treated with compound 2 were assayed for the cleavage of poly-(ADP-ribose)-polymerase and DNA fragmentation resulting in nuclear shrinkage. (unibas.ch)

This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libraries in HeLa cervical cancer cells in 96-well format. (ox.ac.uk)

The biological and antiproliferative activity of the furanocoumarins were examined using human cervical carcinoma HeLa cell line and LNCaP prostatic cell line. (ac.ir)
A morphological analysis of aviprin treated cells revealed that LNCaP cells became fragmented, whereas HeLa cells became condensed. (ac.ir)

When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)

spread of metastatic cells [3]. (researchgate.net)
An aggressive HeLa, a metastatic ME-180 and a primary SiHa cell lines were purchased from ATCC (USA, MD). Camptothecin was supplied by Calbiochem® and cannabidiol was purchased from Sigma-Aldrich and used as a standard reference. (biomedcentral.com)

Cells from the human HeLa (Cervical adenocarcinoma, ATCC CCL 2) cell line were heat-killed by incubation at 60°C (45 min), cooled to room temperature, and mixed with an equal number of untreated viable HeLa cells. (bdbiosciences.com)
We will provide viable cells that proliferate on revival. (abcam.com)

Experiments with endothelial cells fortified with the isolated fraction from native honey enriched in antioxidants, exposed to peroxyl radicals from 1,1-diphenyl-2-picrylhydrazyl (AAPH, 10 mM) and to hydrogen peroxide (H2O2, 50-100 microM), indicated that phenolic acids and flavonoids were the main causes of the protective effect. (researchgate.net)

The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). (knoblauchpublishing.com)

Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)

The results indicated that porous spherical CaCO 3 microparticles may induce oxidative damage to Hela cells. (scirp.org)
The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)

Biocompatibility of composites was tested on HELF and HeLa cells and revealed no toxic effects. (nature.com)
These biological nanopores have been designed to protect the interior of the bacterial cell from leakage of toxic compounds while selectively allowing the entry of the molecules needed for cell growth and function. (ibecbarcelona.eu)
It was hypothesized delta-endotoxin would be highly toxic to HeLa (cervical cancer) cells compared to MCF-7 (breast cancer) cells and served as a good anti-cancer agent. (tnstate.edu)

Acute ozone exposure compromised cell membrane integrity severely, while adaptation to reverse an initial reduction in mitochondrial activity was observed. (nwu.ac.za)

For example, air pollutants can damage the pollen cell wall and facilitate the release of allergenic proteins and other cytoplasmic substances into the environment ( 16 - 20 ). (frontiersin.org)
On the other hand, DYNLT1knockdown experiments 5-ht5 Receptors Inhibitors targets showed a dramatic enhance in sensitivity to hypoxia with a concomitant reduction in cell viability and MMP and mPT damage (Figure 7). (lckinhibitor.com)
After sonoactivation, the cyaninplatin drug showed strengthened mitochondrial DNA damage as a marker of cell killing efficiency. (worldmedicinefoundation.com)

The reactive oxygen species (ROS), glutathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. (scirp.org)
In vivo studies further confirmed the inhibitory effect of celastrol on the expression of HIF-1α proteins, leading to a decreased growth of Hep3B cells in a xenograft tumor model. (spandidos-publications.com)
Virus release from BSR cells is solely dependent on NS3 expression, whereas virus release from KC cells is strongly reduced by either lack of NS3, NS3a or both proteins. (adenosine-receptor.com)
Since virus release from insect cells occurs by budding, these results suggest that both proteins are FCCP site involved in budding, and thus also suggests a specific role for NS3a. (adenosine-receptor.com)
Further research is needed to unravel the specific role of each of these non-structural proteins in virus release from insect cells. (adenosine-receptor.com)

Cell lysates were fractionated and probed for RIPK3 or RAGE. (bioz.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
MAP4 overexpression contributes to cellular viability (measured by MTT) and power metabolism upkeep (measured by ATP) throughout hypoxia. (lckinhibitor.com)

furthermore, their cavitational effect facilitates the release of extractable compounds and enhances the mass transport by disrupting the plant cell walls [ 7 ]. (biomedcentral.com)

To answer this question, we first investigated the ability of ellagic acid to influence the activity of the estrogen receptor subtypes ERalpha and ERbeta in HeLa cells. (nutrimedical.com)

There was even a reduction in cell death. (thermofisher.com)

Subsequently, F.E was fractionated using phase-partitioning with using different cancers cell lines. (columbiagypsy.net)
Note: This protocol was optimized using HeLa cells adhering to coverslips and has been confirmed in other common cell lines. (cytoskeleton.com)
Celastrol showed a potent inhibitory activity against HIF-1 activation induced by hypoxia in various human cancer cell lines. (spandidos-publications.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
In vitro antiproliferative activity of selected medicinal plants from the North-West of Morocco on several cancer cell lines. (jptcp.com)
However, this disturbed virus release is not a complete blockade, since mutant viruses were propagated, and successfully passed in normal cell lines. (adenosine-receptor.com)
Also, PIM cultured in some common cell lines to isolate other viruses of interest may unintentionally amplify poliovirus. (cdc.gov)
Respiratory or enteric viral isolates obtained from PIM specimens using poliovirus-permissive cell lines are also considered PIM. (cdc.gov)

0.01) without toxicity to normal cells. (biomedcentral.com)

Fluorescent carbon dots (CDs) are potential tools for the labeling of cells with many advantages such as photostability, multicolor emission, small size, rapid uptake, biocompatibility, and easy preparation. (knoblauchpublishing.com)

As shown in Figure 1B, MAP4 overexpression can constitutively upregulate tubulin, and, intriguingly, also heightens DYNLT1 expression in CMs and HeLa cells (Figure 4A). (lckinhibitor.com)

Cells exhibiting both positive and negative feedback fluoresced green (live) upon exposure to calcein am, which indicates esterase activity in living cells. (nist.gov)
Cells were exposed to an ozone-saturated physiological solution using various dosing regimens, including acute exposure and various repetitive exposures. (nwu.ac.za)

Obviously, the main mechanism of egress of non-enveloped viruses is cell lysis, therewith most likely killing the infected mammalian cell. (adenosine-receptor.com)
For arthropod borne orbiviruses, cell lysis has not been observed in insect cells in contrast to cell lysis of mammalian cells. (adenosine-receptor.com)
Obviously, NS3 plays an important role in BTV release from mammalian cells, whereas both NS3 and NS3a are important for release from KC cells. (adenosine-receptor.com)

Morphologically live (red solid line histogram) and dead (blue dashed line histogram) HeLa cells were gated based on their distinct forward and side light-scattering characteristics and can be clearly distinguished based on staining intensity of BD Via-Probe™ Green. (bdbiosciences.com)
Human wild-type HeLa cell line ( ab255928 ). (abcam.com)
Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
Moreover, ellagic acid induced nodule mineralization in an osteoblastic cell line (KS483), an effect that was abolished by the estrogen antagonist. (nutrimedical.com)

ER Flipper-TR® is a live cell fluorescent probe that specifically targets the endoplasmic reticulum (ER) membrane of cells and reports membrane tension changes through its fluorescence lifetime changes. (cytoskeleton.com)
ER Flipper-TR® spontaneously localizes to the ER membrane of cells and is only fluorescent when inserted in the lipid membrane. (cytoskeleton.com)
Finally, and with the aim of demonstrating the suitability of this system to be used in pretargeted strategies, functionalized fluorescent NPs were used to label H358 cells with a clickable anti-PD-L1 Ab, applying the reaction between Cl-Syd and DBCO as corresponding clickable groups. (bvsalud.org)

By locally oxidizing ferrocenemethanol (FcCH2OH) at a microelectrode, we probed the metabolic activity of individual HeLa cells using a scanning electrochemical microscope. (nist.gov)

For BSR cells, a lower virus titer in the supernatant and cell-associated fraction was also associated with a significantly delayed but clear CPE and reduced plaque size. (adenosine-receptor.com)

No. R6513) and acquired by flow cytometry at a low flow rate using a BD LSRFortessa™ Cell Analyzer System. (bdbiosciences.com)

Growth analysis was determined using a cell viability indicator, Alamar Blue, and a florescent plate reader. (tnstate.edu)

Tests were carried out in 12-well NUNC plates previously covered with polyornithine (0.1 mg·mL?1) to improve cell attachment. (exposed-skin-care.net)
Within randomly orientated PCL-PLA fibers, HeLa cells exhibited typical shape and attachment with higher than 90% viability prior to freezing. (huji.ac.il)

Anatomy23

Organisms15

Diseases2

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes83

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science3

Health Care3

Cytotoxicity9

Assay10

CERVICAL CANCER CELL LINES3

HepG23

Proliferation8

Epithelial3

Anticancer4

Inhibition9

Cytotoxic2

Apoptosis9

Microscopy1

Concentrations10

A5496

Concentration5

Transfection12

Inhibit1

Extracts4

Compound2

SiRNA1

LNCaP2

Reagent2

Metastatic2

Viable2

Endothelial cells1

Carcinoma1

Adenocarcinoma1

Oxidative2

Toxic3

Membrane integrity1

Damage3

Proteins5

Lysates1

Cellular2

Compounds1

Subtypes1

Reduction in cell1

Lines8

0.011

Biocompatibility1

Overexpression1

Exposure2

Mammalian3

Line4

Fluorescent3

Metabolic1

Fraction1

Flow cytometry1

Indicator1

Attachment2