• Human cells cultured in vitro have thus proven to be extremely useful to scientists studying the molecular and biochemical aspects of human carcinogenesis. (springer.com)
  • These advances have created experimental approaches to answering critical questions in human cell carcinogenesis 1,2 . (springer.com)
  • Sex-determining region Y-box 2 (SOX2) is reported to be up-regulated in other kinds of cancer cells and involved in carcinogenesis and malignancy. (cancerindex.org)
  • While the high effectiveness in cell killing provides the rationale for heavy ion cancer therapy (hadron therapy), residual damage in surviving cells is of concern for carcinogenesis. (wikipedia.org)
  • The mechanisms by which CD44 plays a role in tumor progression are not yet clear although they do seem to be to implicated in cell survival signaling as well as regulation of cellular invasion and metastasis ( 1 , 3 ). (aacrjournals.org)
  • Dysfunction of cell adhesion (metastasis) is characteristic for tumor cells and is regarded as a result of alternation in E-cadherin expression (encoded by CDH1). (termedia.pl)
  • Chemically defined media have been developed for culturing many of these tissues and cells from normal organs, including those with a high rate of cancer in humans. (springer.com)
  • This suggests a cell type-specific reliance on Wip1 for tumorigenesis, because alveolar progenitor cells are the likely target for transformation in the MMTV(mouse mammary tumor virus)-neu but not MMTV-wnt1 breast cancer model. (nih.gov)
  • In the MMTV-neu model, HER2/neu activates STAT5 in alveolar progenitor cells independent of Wip1, but HER2/neu does not override the defect in STAT5 activation in Wip1-deficient hormone-sensing cells, and paracrine stimulation remains attenuated. (nih.gov)
  • Transformation assay of established cell lines : mechanisms and application, proceedings of a workshop organized by IARC in collaboration with the US National Cancer Institute and the US Environmental Protection Agency, held in Lyon, 15-17 February 1984 / editors, T. Kakunaga, H. Yamasaki. (who.int)
  • Polyploid giant cancer cells (PGCCs) are mononucleated or multinucleated cells with multiple genome copies that can be observed in cancer but that can also play an important role in many normal physiologic and nonmalignant pathologic processes [ 10 - 12 ]. (pubmedcentralcanada.ca)
  • In these transformed cells, CD44 was found to undergo a sequential metalloprotease and γ-secretase cleavage, resulting in an increase in expression of CD44-ICD. (aacrjournals.org)
  • Immunofluorescent staining revealed expression of stem cell factors OCT4, Nanog, and SOX-2 in spheroid, whereas expression of embryonic stem cell marker SSEA1 was increased in HRAS -transformed cells compared with their immortalized isogenic counterparts. (pubmedcentralcanada.ca)
  • Some of the previous studies demonstrated the ability of ascites to modulate the gene expression, which is associated with changes in the cellular behavior of ovarian cancer cell [5, (termedia.pl)
  • Through microarray analysis, gene expression of transformed cells were compared against control cells. (omictools.com)
  • It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. (cancerindex.org)
  • The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. (cancerindex.org)
  • AHCC was applied to KLM1-R cells and expression of SOX2 was analyzed by western blotting. (cancerindex.org)
  • Detailed studies reveal that incubation of the irradiated cells during the entire 21-day expression period at pH 6.7-6.8 versus pH 7.0-7.2 significantly suppressed the transformation frequency after 7 Gy, from 4.4 × 10 -4 to 4.6 × 10 -5 (accumulated data). (elsevier.com)
  • In this study, we used the Wip1-knockout mouse to identify the cell types that are dependent on Wip1 expression and therefore may be involved in the early stages of HER2/neu-induced tumorigenesis. (nih.gov)
  • Using a transgenic Drosophila melanogaster model, we show that CagA expression in the simple model epithelium of the larval wing imaginal disc causes dramatic tissue perturbations and apoptosis when CagA-expressing and non-expressing cells are juxtaposed. (nih.gov)
  • CagA-induced apoptosis was enhanced by loss of nTSGs or ectopic expression of the small GTPase Rho1 in the CagA-expressing cells and loss of the TNF homolog Egr in non-expressing cells (Figure 6A). (nih.gov)
  • Here, we took advantage of this reversible transformation system of immortalized rodent fibroblastic cells and found in a restricted proteomic analysis that standard CD44 is one of the main up-regulated protein associated with detergent-resistant membranes (DRM) during the transformation process. (aacrjournals.org)
  • The complexity and heterogeneity of cancer cells are under-represented by the current experimental models which revolve around primary tumor cells, cancer cell lines, and rodent models. (omictools.com)
  • In addition, activator protein-1 activity in H3 WT-overexpressing cells was markedly up-regulated by EGF in contrast to the H3 mutant S10A or S28A cells. (aacrjournals.org)
  • Immunohistochemically, a few spindle cells surrounding the ductal structures showed immunopositivity for alpha-smooth muscle actin and S-100 protein. (biomedsearch.com)
  • Basaloid carcinoma showed a gradual transition to chondrosarcomatous cells producing the matrix, which had both immunopositivities for S-100 protein and cytokeratin. (biomedsearch.com)
  • One group of tumors, designated type I, is composed of low-grade serous, low-grade endometrioid, clear cell, mucinous and transitional (Brenner) carcinomas. (nih.gov)
  • Endometrioid and clear cell tumors have been associated with endometriosis that is regarded as the precursor of these tumors. (nih.gov)
  • Normal and neoplastic hematopoiesis : proceedings of the UCLA Symposium held at Steamboat Springs, Colorado, March 27-April 1, 1983 / editors, David W. Golde, Paul A. Marks. (who.int)
  • Hormone-sensing cells require Wip1 for paracrine stimulation in normal and premalignant mammary epithelium. (nih.gov)
  • The molecular circuitry of different cell types dictates their normal function as well as their response to oncogene activation. (nih.gov)
  • A significant increase in the density of mast cells has been seen in cherry hemangiomas compared with normal skin. (wikipedia.org)
  • The instructive role of hormone-sensing cells in premalignant development suggests targeting Wip1 or prolactin signaling as an orthogonal strategy for inhibiting breast cancer development or relapse. (nih.gov)
  • Because mitosis is the phase of the cell cycle most susceptible to various chemical and physical agents, the consistent observation of histone H3 phosphorylation at Ser 10 and/or overexpression in human tumor cell lines ( 20 ) suggested that regulation of mitosis by phosphorylation of histone H3 at Ser 10 may be a possible target for cancer treatment. (aacrjournals.org)
  • This result suggests that the target of AHCC in expressing therapeutic efficacy was not the pluripotent cells such as cancer stem cells (CSCs) but SOX2-specific. (cancerindex.org)
  • This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. (cancerindex.org)
  • Furthermore, transformed cells exhibited increased telomerase activity that was not accountable by hTERT overexpression alone. (omictools.com)
  • Indications are that: for high-LET radiation, where complex DSBs occur with high frequency, little repair occurs, leading to cell death or the mis-rejoining of unrepairable ends with other radiation-induced DSB leads to large DNA deletions and chromosome aberrations. (wikipedia.org)
  • When H3 WT was overexpressed, EGF induction of c- fos and c- jun promoter activity was significantly increased compared with control cells but not in the H3 mutant S10A or S28A cells. (aacrjournals.org)
  • Furthermore, our findings highlight that hormone-sensing cells convert not only estrogen and progesterone but also prolactin signals into paracrine instructions for mammary gland development. (nih.gov)
  • Electron microscopy revealed that the spindle cells had tonofilament and pinocytic vesicles in the cytoplasm, and basal lamina adjacent to the cytoplasmic membrane. (biomedsearch.com)
  • Vice versa, cell surface molecules are suggested to mediate extracellular signals to the genome. (biomedsearch.com)
  • We discuss possible mechanisms for the observed suppression, in terms of the influence of low extracellular pH on cell turnover, repair of radiation damage, cell toxicity, and activity of cellular proteases. (elsevier.com)
  • Mendonca, M , Sun, C & Redpath, JL 1990, ' Suppression of radiation-induced neoplastic transformation of human cell hybrids by long term incubation at low extracellular pH ', Cancer Research , vol. 50, no. 7, pp. 2123-2127. (elsevier.com)
  • Moreover, ERK (extracellular signal-regulated kinase) activation by HER2/neu in hormone-sensing cells is also Wip1 dependent. (nih.gov)
  • F. L. Graham, J. Smiley, W. C. Russell and R. Nairn, Characteristics of a human cell line trasformed by DNA from human adenovirus type 5, J. Gen. Virol. (springer.com)
  • Unexpectedly, Wip1 allows steroid hormone-receptor-positive cells but not alveolar progenitors to activate STAT5 (signal transducer and activator of transcription 5) in the virgin state. (nih.gov)
  • Tools from evolutionary theory have the potential to parse those mutations that are selected within cells on the path to cancer from those mutations that arise incidentally during the somatic evolution of cancer. (yale.edu)
  • We used the transformed cell model in our studies. (omictools.com)
  • Collectively, these data imply that IMR90 transformed cell model is an invaluable tool for cancer studies. (omictools.com)
  • In fact, only a small fraction of the initial damage is transduction of late chromosomal damage has also been measured in the progeny of human lymphocytes that were exposed with much higher frequency in the progeny of cells that were exposed to heavy ions compared to gamma rays. (wikipedia.org)