Cell transcription factors. Medical search. Frequent questions

Real-time qRT-PCR results showed that the local delivery of SATB2-overexpressing BMSCs enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins in the implantation sites. (nih.gov)
Membrane proteins implicated in long-chain fatty acid uptake by mammalian cells: CD36, FATP and FABPm. (springer.com)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
Transcription factors are proteins that regulate the transcription of genes, or the production of mRNA from DNA. (biolegend.com)
GS-002 also induced endoplasmic reticular (ER) stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153), phosphorylated eukaryotic initiation factor 2 α (eIF2 α ), phosphorylated protein endoplasmic-reticular-resident kinase (PERK), and ATF-3. (hindawi.com)
Stroboscopic photo-activation (SPA) coupled with single particle tracking (SPT) constitutes a powerful method to probe the intracellular journey of proteins in a living cell with single molecule resolution. (monash.edu)
Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
Essential FA, mainly polyunsaturated fatty acids (PUFA), may modulate gene expression in diverse biological processes thought regulating transcription factors (TF), including peroxisome proliferator receptors (PPAR) , liver X receptors (LXR) , and sterol regulatory element-binding proteins ( SREBP ) 3 . (nature.com)
MIXTA/MIXTA-like proteins, initially described in snapdragon (Antirrhinum majus) petals, are known regulators of epidermal cell differentiation. (fmach.it)
functional" genes encoding proteins such as late embryogenesis-associated proteins, detoxification enzymes, heat shock proteins and molecular chaperones, which directly protect plants from abiotic stress, and "regulatory" genes encoding proteins such as protein kinases and transcription factors (TFs), which have roles in the perception and transduction of stress signals. (biomedcentral.com)
The NAC domain was deduced from three previously characterized proteins, petunia NAM (no apical meristem), Arabidopsis thaliana ATAF1/2 ( Arabidopsis thaliana activation factor 1/2) and CUC2 (cup-shaped cotyledon) [ 13 , 14 ]. (biomedcentral.com)
We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
Amongst other factors, one group of proteins called transcription factors (TFs) is crucial for regulating whether a cell will develop into, for example, a liver or a nerve cell. (mdc-berlin.de)
When STAT proteins are activated, they can increase the transcription of several target genes , which leads to unchecked cellular proliferation , anti-apoptotic reactions, and angiogenesis. (bvsalud.org)
Menin interacts with many other proteins, including several transcription factors. (medlineplus.gov)
Modulation of Basic Helix-Loop-Helix Transcription Complex Formation by Id Proteins during Neuronal Differentiation. (lu.se)
It is assumed that the Id helix-loop-helix (HLH) proteins act by associating with ubiquitously expressed basic HLH (bHLH) transcription factors, such as E47 and E2-2, which prevents these factors from forming functional hetero- or homodimeric DNA binding complexes. (lu.se)
We found that the three Id proteins expressed in neuroblastoma cells (Id1, Id2, and Id3) were down-regulated during induced differentiation, indicating that Id proteins help keep the tumor cells in an undifferentiated state. (lu.se)
The ability of Id proteins to affect HES-1 activity is of particular interest in neuronal cells, where regulation of HES-1 is essential for the timing of neuronal differentiation. (lu.se)

Transcription factor MEF2C influences neural stem/progenitor cell differentiation and maturation in vivo. (ca.gov)
Here, we show that conditional knockout of Mef2c in nestin-expressing neural stem/progenitor cells (NSCs) impaired neuronal differentiation in vivo, resulting in aberrant compaction and smaller somal size. (ca.gov)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
STAT3 protein belongs to a group of intracellular transcription factors that mediate a variety of functions such as cellular differentiation, proliferation, and apoptosis. (biolegend.com)
Here we report that simultaneous overexpression of two transcription factors (TFs) can direct the differentiation of human iPSCs to granulosa-like cells. (biorxiv.org)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repression and transcriptional activation. (lu.se)
However, the molecular mechanisms which regulate Th1 cell differentiation in CD mucosa are unknown. (bmj.com)
Secretory epithelial cells display lactogenic differentiation which is characterized by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
Transcription factors AP-2alpha and AP-2gamma are downregulated during lactation, and their overexpression in transgenic mice impaired the secretory differentiation of the mammary epithelium, resulting in lactation failure. (biomedcentral.com)
To explore whether the downregulation of AP-2alpha and AP-2gamma is of functional significance for lactogenic differentiation, we analyzed the expression of the AP-2 family members during the lactogenic differentiation of HC11 mammary epithelial cells in vitro. (biomedcentral.com)
Differentiation of HC11 cells was induced following established protocols by applying the lactogenic hormones prolactin, dexamethasone and insulin. (biomedcentral.com)
Using RT-PCR we could not detect a downregulation of any of these genes during the lactogenic differentiation of HC11 cells in vitro. (biomedcentral.com)
The extent of lactogenic differentiation of HC11 cells in vitro is limited compared to mammary epithelium undergoing secretory differentiation in vivo. (biomedcentral.com)
Downregulation of AP-2 transcription factor genes is not required for lactogenic differentiation of HC11 cells but may functionally be involved in aspects of lactogenic differentiation in vivo that are not reflected by the HC11 system. (biomedcentral.com)
The lactogenic differentiation of the secretory epithelial cells is characterized by the synthesis of lactose and milk fat and by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
HC11 cells can be induced to undergo lactogenic differentiation by applying a mixture of the lactogenic hormones dexamethasone, insulin and prolactin (a mixture termed DIP in the following sections). (biomedcentral.com)
In this report we analyzed AP-2 transcription factor expression in HC11 cells subjected to lactogenic differentiation conditions. (biomedcentral.com)
For induction of differentiation, cells were grown to confluency and then kept in medium without EGF for 48 hours to induce competence. (biomedcentral.com)
AP-1 transcription factor has been shown to have a hand in a wide range of cellular processes, including cell growth, differentiation, and apoptosis. (wikipedia.org)
Transcription factor Sp2 promotes TGFB-mediated interstitial cell osteogenic differentiation in bicuspid aortic valves through a SMAD-dependent pathway. (nih.gov)
FOXP2 mRNA and protein were absent in mononuclear cells from different anatomical sites, lineages and stages of differentiation. (ox.ac.uk)
To further analyze RhoA function in thyroid cell proliferation and differentiation patterns, we combined transient and stable transfection assays to express different mutant RhoA forms in FRTL-5 cells. (unina.it)
Interestingly, FRTL-5-RhoA QL cells express less Tg and TTF-1 than wild-type FRTL-5 (FRTL-5-vector) or FRTL-5-RhoA N19, suggesting a loss at the differentiation stage. (unina.it)
Taken together, these results show for the first time that active RhoA is able to transform FRTL-5 cells and that this effect is coupled to a loss of thyroid differentiation due to impaired TTF-1 activity. (unina.it)
While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)
Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
Small cell carcinoma of the prostate is a high-grade malignant neoplasm with neuroendocrine differentiation. (medscape.com)
that regulate the differentiation and activation of these cells. (cdc.gov)

The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
Differentiating HC11 cells displayed lower expression levels of milk protein genes than mammary glands of mid-pregnant or lactating mice. (biomedcentral.com)
FOXP2 represents the first transcription factor whose expression consistently differentiates normal and abnormal plasma cells and FOXP2 target genes are implicated in MM pathogenesis. (ox.ac.uk)
Highly specialized cells, the thyrocytes, express a thyroid-specific set of genes for thyroglobulin (Tg), thyroperoxidase, and the transcription factors TTF-1, TTF-2, and Pax-8. (unina.it)
The emphasis of these studies has been to model the relationships between transcription factors and their target genes. (biomedcentral.com)
Using chromatin immunoprecipitation in conjunction with microarrays, it is now possible to measure the binding of many transcription factors to the promoters of most genes, thus elucidating the underlying structure of the transcriptional regulatory network. (biomedcentral.com)
The simplest models identify transcription factor binding sites (or cis regulatory motifs) that are over-represented in the promoters of differentially-expressed genes [ 9 ]. (biomedcentral.com)
Duplication of genes and their association with different cis -regulatory regions may therefore result in the specification of novel plant cell types. (biologists.com)
They are caused by genetic translocations which fuse transcription factor genes, TFE3 or TFEB, with a promoter region leading to overexpression of the transcription factor. (dundee.ac.uk)
Our genome-wide analysis found 151 NAC transcription factor genes ( PgNAC s) in the pearl millet genome. (biomedcentral.com)
The pearl millet genome contains 151 NAC transcription factor genes that can be classified into 11 groups. (biomedcentral.com)
Genes to Cells , 12 (9), 983-996. (elsevierpure.com)
Many TFs act by docking onto certain sections in the DNA and regulating the expression (i.e. transcription) of nearby genes. (mdc-berlin.de)
Combinatorial regulation thereby allows hundreds of TFs to control the spatio-temporal transcription of tens of thousands of genes - a prerequisite for the proper development and existence of an organism. (mdc-berlin.de)
Transcription factors bind to specific areas of DNA and help control whether particular genes are turned on or off. (medlineplus.gov)
Some of these genes likely play a role in cell growth and division. (medlineplus.gov)

Changes in dietary fatty acids, specifically the polyunsaturated fatty acids of the ω-3 and ω-6 families and some derived eicosanoids from lipoxygenases, cyclooxygenases, and cytochrome P-450, seem to control the activity of transcription factor families involved in cancer cell proliferation or cell death. (springer.com)
We report that haploinsufficiency for the forkhead transcription factor Foxo1 reverses beta cell failure in Irs2(-/-) mice through partial restoration of beta cell proliferation and increased expression of the pancreatic transcription factor pancreas/duodenum homeobox gene-1 (Pdx1). (nih.gov)
We propose that insulin/IGFs regulate beta cell proliferation by relieving Foxo1 inhibition of Pdx1 expression in a subset of cells embedded within pancreatic ducts. (nih.gov)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
The AP-1 transcription factor has been shown to play numerous roles in cell growth and proliferation. (wikipedia.org)
C-jun has been shown to be essential for fibroblast proliferation, and levels of both AP-1 subunits have been shown to be expressed above basal levels during cell division. (wikipedia.org)
The growth factors TGF alpha, TGF beta, and IL2 have all been shown to stimulate c-Fos, and thereby stimulate cellular proliferation via AP-1 activation. (wikipedia.org)
Transcription Factor SP2 Regulates Ski-mediated Astrocyte Proliferation In Vitro. (nih.gov)
FOXP2 mRNA was expressed in bone marrow samples from 96% of MM patients (24/25), 66.7% of patients with the pre-neoplastic plasma cell proliferation monoclonal gammopathy of undetermined significance (MGUS) (6/9), but not in reactive plasma cells. (ox.ac.uk)
The implication of the small GTPase RhoA in TSH-mediated proliferation of FRTL-5 rat thyroid cells has been previously demonstrated. (unina.it)
Signal transducer and activator of transcription (STAT) family of transcription factors are involved in lung cancer cell proliferation , metastasis , immunological control, and treatment resistance. (bvsalud.org)

The current study assessed the effects of bone marrow stromal cells (BMSCs) with forced expression of special AT-rich sequence-binding protein 2 (SATB2) on the osseointegration of titanium implants. (nih.gov)
Their regulation may be carried out either through direct binding to DNA as peroxisome proliferator-activated receptors or via modulation in an indirect manner of signaling pathway molecules (e.g., protein kinase C) and other transcription factors (nuclear factor kappa B and sterol regulatory element binding protein). (springer.com)
I will especially focus on new methods developed in my laboratory for the visualization of protein interactions in living cells. (hstalks.com)
Thus, cells respond to their environment by altering protein interactions. (hstalks.com)
Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
The encoded protein contains two different binding sites that are known to bind DNA and function as a regulator of gene transcription. (biolegend.com)
Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
Activator protein 1 (AP-1) is a transcription factor that regulates gene expression in response to a variety of stimuli, including cytokines, growth factors, stress, and bacterial and viral infections. (wikipedia.org)
PREDICTED: transcription factor-like 5 protein [Pan paniscus]. (bioinformatics.org)
FOXP2 mRNA and protein expression were characterised in normal human tissues, haematological cell lines and multiple myeloma (MM) patients' samples. (ox.ac.uk)
However, FOXP2 mRNA and protein was detected in several lymphoma (8/20) and all MM-derived cell lines (n = 4). (ox.ac.uk)
Protein expression was investigated by ELISA and immunofluorescence, transcription factor-DNA binding by ELISA, gene expression by real-time PCR, prostaglandin E 2 (PGE 2 ) by radioimmunoassay, and matrix metalloproteinase (MMP) activity and nitric oxide (NO) production by fluorometry. (karger.com)
Results: H 2 O 2 challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II, increased number of autophagosomes and autolysosomes while decreased protein abundance of p62. (illinois.edu)
First, the general TFs (GTFs), including preinitiation complex components TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and THIIH, are the primary protein factors that are required for the initiation of transcription from the TATA box (or TATA element), then elongation is executed by RNA polymerase II (RNA pol II) [ 1 ]. (intechopen.com)
This protein acts as a tumor suppressor, which means that it keeps cells from growing and dividing too fast or in an uncontrolled way. (medlineplus.gov)
The menin protein is present in the nucleus of many different types of cells and appears to be active in all stages of development. (medlineplus.gov)
As a result of these mutations, one copy of the MEN1 gene in each cell makes no functional protein. (medlineplus.gov)
[ 17 , 18 ] TCF7L2 protein is increased during adipogenesis in 3T3-L1 cells and in primary adipocyte stem cells. (medscape.com)
A cardiomelic developmental field has also been postulated to relate the genetic heterogeneity of HOS (and other similar syndromes) to a cascade of molecules, including the brachyury, sonic hedgehog, bone morphogenetic protein, retinoic acid receptor, and transforming growth factor beta families. (medscape.com)
Activation of the transcription factor nuclear factor KB (NF-KB) requires the NEMO protein. (medscape.com)

Knowledge of the mechanisms by which fatty acids control specific gene expression may identify important risk factors for cancer and provide insight into the development of new therapeutic strategies for a better management of whole body lipid metabolism. (springer.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. (biolegend.com)
The identified transcription factors influence both the global and specific gene expression of the BCLs and have possible implications for diagnosis and treatment. (lu.se)
This one of a kind research infrastructure would enable scientists in Australia to image for the first time the biophysical mechanism by which a transcription factor navigates the nuclear architecture of a living cell and the kinetics of transcription factor interaction with a DNA target sequence that lead to a specific gene expression program. (monash.edu)
One of the great missing pieces in our understanding of gene expression is how transcription factors regulate and control it in an orchestrated fashion. (genomeweb.com)
AP-1 transcription is deeply involved in the modulation of gene expression. (wikipedia.org)
However, it is likely that transcription factor binding affects gene expression in a nonlinear fashion, e.g., below some level it has no effect and above some higher level the effect might saturate. (biomedcentral.com)
Combinatorially with other TFs, they control which information is expressed and since different cells contain different sets of TFs, they also feature different gene expression programs. (mdc-berlin.de)

Here we show that the T-box transcription factor Brachyury induces in tumor cells epithelial-mesenchymal transition (EMT), an important step in the progression of primary tumors toward metastasis. (jci.org)
We have shown that AUF1 induces EMT and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation. (nature.com)
The signal transduction network induces transcription factor interactions, both off DNA and upon assembly at specific regulatory elements. (hstalks.com)
Transforming growth factor-beta (TGF-beta) signaling in naive T cells induces expression of the transcription factor Foxp3, a 'master' regulator of regulatory T cells (T(reg) cells). (nih.gov)

This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. (nih.gov)

Foxo1 and Pdx1 exhibit mutually exclusive patterns of nuclear localization in beta cells, and constitutive nuclear expression of a mutant Foxo1 is associated with lack of Pdx1 expression. (nih.gov)
This nuclear transcription factor plays an important role in embryologic development of the lung, as well as carcinogenesis of lung cancer. (iiarjournals.org)
Tumor cells were considered positive only if distinct nuclear staining was identified ( 5 , 8 ). (iiarjournals.org)
According to a previous report, the tumor samples were considered TTF1-positive if at least 5% of the tumor cells exhibited nuclear expression ( 5 ). (iiarjournals.org)
Molecular mechanisms of the initiation of transcription from TATA box have been well known as the most essential nuclear events in mammalian cells. (intechopen.com)
In 2000, the International Incontinentia Pigmenti Consortium reported that incontinentia pigmenti is caused by a genomic rearrangement of the gene for NEMO, or nuclear factor kappa B essential modulator (IKBKG-IKK gamma). (medscape.com)
Cell reprogramming can be achieved experimentally in different ways, including nuclear transfer, cell fusion or expression of transcription factors. (lu.se)
The morphologic features characteristic of the disease's pathology include small tumor cells with minimal cytoplasm, nuclear molding, fine chromatin pattern, extensive tumor necrosis/apoptosis, and a brisk mitotic rate. (medscape.com)

The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. (bdbiosciences.com)
RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
In the skin, NF-KB regulates cell growth in the stratified epithelium and apoptosis. (medscape.com)

Several studies have clearly demonstrated that distinct cytokine activated signalling and transcription factors regulate the commitment of a naïve T cell along the Th1 or T helper cell type 2 (Th2) phenotype, as well as maintenance of the polarised phenotype. (bmj.com)
In contrast, here we focus on reverse-engineering the network of relationships among transcription factors that regulate the cell cycle in S. cerevisiae . (biomedcentral.com)
We have developed a technique to reverse-engineer networks of the time-dependent activities of transcription factors that regulate the cell cycle in S. cerevisiae . (biomedcentral.com)
Does the binding of a specific transcription factor to the promoter of a gene regulate its expression? (biomedcentral.com)

abstract = "Translocation renal cell carcinomas (tRCC) are a novel, rare, and distinct clinicopathologic entity first described in 2001. (dundee.ac.uk)

Overexpression of Brachyury in human carcinoma cells induced changes characteristic of EMT, including upregulation of mesenchymal markers, downregulation of epithelial markers, and an increase in cell migration and invasion. (jci.org)
Since epithelial-to-mesenchymal transition (EMT) and the acquisition of cancer stem cell traits are important for cancer onset and progression, we sought to determine the role of AUF1 in these two important processes. (nature.com)
Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
These data prompted us to ask whether AUF1 upregulation could promote EMT as well as stemness in mammary epithelial cells. (nature.com)
Background: Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy. (illinois.edu)
However, effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain unknown. (illinois.edu)
Therefore, the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative stress. (illinois.edu)
Other particles may be engulfed by epithelial cells, primarily in the vicinity of the bronchial-alveolar duct junctions, and retained for much longer periods, with gradual removal to lymph nodes. (cdc.gov)
http://dx.doi.org/10.1016/j.jaci.2014.02.015 epithelial cells and airway smooth muscle cells. (cdc.gov)

An extended transcriptional network for pluripotency of embryonic stem cells. (bdbiosciences.com)
Nanog and transcriptional networks in embryonic stem cell pluripotency. (bdbiosciences.com)
We validate our approach in two ways: by demonstrating that it accurately models expression data and by demonstrating that our reconstructed model is similar to previously-published models of transcriptional regulation of the cell cycle. (biomedcentral.com)
Our regression-based approach allows us to build a general model of transcriptional regulation of the yeast cell cycle that includes additional factors and couplings not reported in previously-published models. (biomedcentral.com)
A variety of methods have been developed that utilize both expression data and transcription factor binding data to model transcriptional regulation. (biomedcentral.com)
To this end, we overexpressed key transcriptional regulators of astroglial development in human GBM and GBM stem cell lines. (lu.se)
Given that OKSM (Yamanaka) factors convert somatic cells into induced pluripotent stem (iPS) cells, alterations in transcriptional state could affect destiny of the cells. (intechopen.com)
In differentiating neuroblastoma cells, HASH-1 is down-regulated, and there is coincident up-regulation of the transcriptional repressor HES-1, which is known to bind the HASH-1 promoter. (lu.se)

Furthermore, we found Brachyury to be overexpressed in various human tumor tissues and tumor cell lines compared with normal tissues. (jci.org)
However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
Aim: To investigate the impact of tumor-cell expression of thyroid transcription factor 1 (TTF1) on outcomes after irradiation of limited-disease small cell lung cancer (LD-SCLC). (iiarjournals.org)
Particularly in patients presenting with adenocarcinoma, tumor-cell expression of TTF1 was associated with improved overall survival ( 6 ). (iiarjournals.org)

For instance, a previous study by the authors validated that USF1 binds to the core promoter of APOBEC3G and increases its transcription level in hepatocytes ( 7 ). (spandidos-publications.com)
To study the functional properties of hematopoietic cells expressing SCL, we have generated mutant mice (SCLlacZ/w) in which the Escherichia coli lacZ reporter gene has been "knocked in" to the SCL locus, thereby linking beta-galactosidase expression to transcription from the SCL promoter. (ozgene.com)
AP-1 was first discovered as a TPA-activated transcription factor that bound to a cis-regulatory element of the human metallothionein IIa (hMTIIa) promoter and SV40. (wikipedia.org)
Downregulation of NFAT3 expression in T cells is mediated by lower chromatin accessibility and enhancer activity in its promoter in comparison with aortic smooth muscle cells expressing endogenous NFAT3. (aai.org)
The binding sites of T-box transcription factor TBX5 and NK-2 transcription factor-related locus 5 Nkx2.5, which were expressed at higher levels in aortic smooth muscle cells than in T cells, were located within the −387 to +97 NFAT3 promoter region, exhibiting the maximum enhancer activity. (aai.org)

Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. (bdbiosciences.com)

Ovarian development requires the combination of germ cells and their supporting somatic cells, known as granulosa cells. (biorxiv.org)
The emergent ability to directly reprogram somatic cells into desired hematopoietic cell-types is opening avenues to the discovery of new therapies for immune and blood diseases. (lu.se)

Aberrant expression of the neuronal transcription factor FOXP2 in neoplastic plasma cells. (ox.ac.uk)

Brachyury overexpression also repressed E-cadherin transcription, an effect partially mediated by Slug. (jci.org)
We elucidate the regulatory effects of several granulosa-related TFs, and establish that overexpression of NR5A1 and either RUNX1 or RUNX2 is necessary and sufficient to generate granulosa-like cells. (biorxiv.org)
In human CD4 + T cells, overexpression of NFAT1 and NFAT3 enhanced and suppressed IL-2 expression, respectively. (aai.org)
Other tumors also driven by overexpression of members of the MiT family include alveolar soft part sarcoma, clear cell sarcoma and 30%-40% of melanomas. (dundee.ac.uk)

BMC Cell Biology, 9 , 45. (springer.com)
Nature Cell Biology. (bdbiosciences.com)
1 Division of Cell Biology, La Jolla Institute for Allergy and Immunology, La Jolla, California 92037, USA. (nih.gov)
Group Leader at Department of Cell and Regenerative Biology at the University of Wisconsin-Madison, School of Medicine and Public Health, USA. (igbmc.fr)

Boiani M, Schöler HR. Regulatory networks in embryo-derived pluripotent stem cells. (bdbiosciences.com)
Of these approaches, several have focused on the reconstruction of the cell cycle regulatory network of Saccharomyces cerevisiae . (biomedcentral.com)
As part of a larger regulatory network of epidermal cell patterning and L1-layer identity, we found that SlMIXTA-like acts downstream of SlSHINE3 and possibly cooperates with homeodomain Leu zipper IV transcription factors. (fmach.it)
Fine-tuning of transcription, such as when exactly, where exactly, and how strongly a gene is expressed, is determined to a large extent via the exact interactions of TFs with the nearby DNA elements they bind to - the cis -regulatory elements (CRMs). (mdc-berlin.de)

Our data also suggested that expression of SCL was transient and confined to hematopoietic stem and/or progenitor cells, because the differentiated progeny of most lineages (except the erythroid) were beta-galactosidase-negative. (ozgene.com)
it is involved in the development of a wide variety of cell lineages and organs. (medscape.com)

CgNLP1 was shown to target a R2R3 type transcription factor HbMYB8-like in rubber tree, which localized on nucleus and induced necrosis in N. benthamiana . (biorxiv.org)
The centrosome normally stayed at the most apical side, apart from the nucleus, in the neuroepithelial cell during the S to G2 phase, while the Pax6 mutant showed unstable movement of the centrosome associated with an abnormal INM. (elsevierpure.com)
ISGF3 is assembled in the CYTOPLASM and translocated to the CELL NUCLEUS in response to INTERFERON signaling. (bvsalud.org)

In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

Seven and twenty-one days after implantation, the femora were isolated for immunohistochemical (IHC) staining, haematoxylin eosin (H&E) staining, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), and micro-computed tomography (μCT) analysis. (nih.gov)
Interferon γ (IFN-γ) and interleukin (IL)-4 RNA expression was examined in T lamina propria lymphocytes by quantitative reverse transcription-polymerase chain reaction. (bmj.com)

Huh7 cells that overexpress USF1 were used with whole transcriptome profiling through RNA sequencing and chromatin immunoprecipitation (ChIP) sequencing methods to investigate the downstream targets of USF1. (spandidos-publications.com)
Researchers have discovered that chromatin accessibility is heavily influenced by a key transcription factor that is linked to cancer. (frontlinegenomics.com)

Reverse transcription‑quantitative PCR was then used to validate the downstream targets. (spandidos-publications.com)
The expression of 36 randomly selected PgNAC s were examined by quantitative reverse transcription-PCR. (biomedcentral.com)

Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
Inhibition of autophagy via bafilomycin A1 aggravated H 2 O 2 -induced reactive oxygen species (ROS) accumulation and apoptosis in MAC-T cells. (illinois.edu)
For example, it may play a role in copying and repairing DNA and regulating the controlled self-destruction of cells (apoptosis). (medlineplus.gov)
[ 6 ] NF-KB protects cells from apoptosis in response to tumor necrosis factor-alpha (TNF-alpha). (medscape.com)

RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
These data provide a functional correlation between SCL expression and colony-forming ability in immature hematopoietic cells. (ozgene.com)
Second-line chemotherapy regimens vary, depending on whether hematopoietic stem cell transplantation (HSCT) is being considered. (medscape.com)
The focus of our laboratory is to understand the molecular determinants underlying cell reprogramming and hematopoietic specification. (lu.se)
Our approach focuses on Hematopoietic Stem Cells (HSCs) for their remarkable regenerative potential and Dendritic Cells (DCs) as key orchestrators of immunity. (lu.se)

A MYB-related transcription factor (MIXTA) that controls development of conical cell form is expressed only in the inner epidermis of Antirrhinum petals. (biologists.com)

The selective expression of Brachyury in tumor cells and its role in EMT and cancer progression suggest that Brachyury may be an attractive target for antitumor therapies. (jci.org)
Importantly, breast cancer cells that ectopically express AUF1 were more efficient in forming orthotopic tumor xenografts in nude mice than their corresponding controls with limiting cell inocula. (nature.com)
On the other hand, AUF1 downregulation with specific siRNA inhibited EMT and reduced the stemness features in breast cancer cells. (nature.com)
Moreover, AUF1 knockdown sensitized breast cancer cells to the killing effect of cisplatin. (nature.com)
Together, these findings provide clear evidence that AUF1 is an important inducer of the EMT process through stabilization of SNAIL1 and TWIST1 and the consequent promotion of breast cancer stem cells. (nature.com)
In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
Small cell lung cancer (SCLC) represents 10% to 25% of all lung cancer and is characterized by rapid growth, early locoregional recurrence and early distant metastases ( 1 - 3 ). (iiarjournals.org)
Recent studies investigated the prognostic value of thyroid transcription factor 1 (TTF1) expression in non-small cell lung cancer (NSCLC) ( 4 - 7 ). (iiarjournals.org)
FOXP2 mutation causes a severe inherited speech and language defect, while the related transcription factors FOXP1, FOXP3 and FOXP4 are implicated in cancer. (ox.ac.uk)
Objective: The cancer stem-like cells (CSC) CSC markers and the role derived from papillary thyroid carcinoma (PTC) in the pathogenesis is unclear. (oct-4.com)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)
Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
Lung cancer treatment potential and limits associated with the STAT family of transcription factors. (bvsalud.org)
Other lifestyle factors, such as sexual practices and occupation, are currently not considered important with regard to the development of prostate cancer. (medscape.com)

Emerging evidence suggests that myocyte enhancer factor 2 (MEF2) transcription factors act as effectors of neurogenesis in the brain, with MEF2C the predominant isoform in developing cerebrocortex. (ca.gov)
Once the transcription factor binds to an enhancer region, this can cause stimulation or repression of gene transcription. (biolegend.com)

This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

These data indicate that conical cells and trichomes are produced by a common developmental pathway. (biologists.com)

Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)

To better understand the role of dietary FA on metabolic diseases, for the first time, a study to identify key transcription factors (TF) involved in lipid metabolism and inflammatory response by transcriptome analysis from liver samples of animal models was performed. (nature.com)

We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)

Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)

Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. (bdbiosciences.com)

Hence, SlMIXTA-like is a positive regulator of both cuticle and conical epidermal cell formation in tomato fruit, acting as a mediator of the tight association between fruit cutin polymer formation, cuticle assembly, and epidermal cell patterning. (fmach.it)

Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei. (rupress.org)
We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. (rupress.org)

To analyse expression of transcription factors which control the Th1 cell commitment in CD. (bmj.com)

The E3 ubiquitin ligase Itch associated with and promoted conjugation of ubiquitin to the transcription factor TIEG1. (nih.gov)

As a transcription factor, Pax6 acts at the molecular level in the signaling and formation of the central nervous system 11 . (biolegend.com)
Upstream transcription factor 1 (USF1) is a canonical transcription factor (TF) and is associated with the pathogenesis of several cancers, but its biological functions and molecular targets in HCC remain unclear. (spandidos-publications.com)

Mice lacking insulin receptor substrate-2 (Irs2) develop beta cell failure, suggesting that insulin signaling is required to maintain an adequate beta cell mass. (nih.gov)
Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZhigh, lacZint, and lacZneg). (ozgene.com)
Functionally, 'TGF-beta-converted' T(reg) cells generated from TIEG1-deficient mice were unable to suppress airway inflammation in vivo. (nih.gov)

Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. (bdbiosciences.com)
Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)

Pax6 is a transcription factor present during embryonic development. (biolegend.com)
How do cells decide what they are going to be over the course of embryonic development, and how do they get there? (mdc-berlin.de)
The skin manifestations occur along the lines of Blaschko, which represent the routes of embryonic cell migration. (medscape.com)

Upstream transcription factor (USF) 1 belongs to the basic helix-loop-helix leucine zipper family and serves as a cellular transcription factor (TF). (spandidos-publications.com)
AP-1 transcription factor is assembled through the dimerization of a characteristic bZIP domain (basic region leucine zipper) in the Fos and Jun subunits. (wikipedia.org)

Nat Rev Mol Cell Biol. (bdbiosciences.com)
J Cell Biol (1996) 133 (5): 929-941. (rupress.org)
Mol Cell Biol. (harvard.edu)

Conversely, inhibition of Brachyury resulted in downregulation of mesenchymal markers and loss of cell migration and invasion and diminished the ability of human tumor cells to form lung metastases in a xenograft model. (jci.org)
NFAT3 is rarely expressed in T cells, and the mechanisms and significance of the specific NFAT3 downregulation in T cells have been unknown. (aai.org)
NFAT3 downregulation in Jurkat cells using RNA interference technology augmented IL-2 expression, whereas a knockdown of NFAT1, NFAT2, and NFAT4 suppressed it. (aai.org)
TBX5 deficiency-mediated downregulation of NFAT3 is crucial for the high cytokine-producing activity of T cells. (aai.org)

Here we show that Itch-/- T cells were resistant to TGF-beta treatment and had less Foxp3 expression. (nih.gov)

This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. (nih.gov)
The responsible gene has been mapped to band 12q24.1, which encodes the human transcription factor TBX5. (medscape.com)

Fatty acids are the main compound of lipids, which are a class of molecules present in animals and vegetal cell types. (nature.com)
Primary source (p.2251 left column 2nd paragraph) gives range of 3,000 to 5,000 molecules/cell. (harvard.edu)

Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
Cellular lysosomal degradative activity is key for healthy cell maintenance. (frontlinegenomics.com)
As soon as transcription starts in the fruit fly embryo, the process of cellular specification commences and within only a few hours a pool of pluripotent cells develops into differentiated components of the nervous system. (mdc-berlin.de)

These findings suggest a functional role of AP-2 transcription factors in preventing lactogenesis. (biomedcentral.com)
If the second copy of the MEN1 gene is also altered, the cell has no working copies of the gene and does not produce any functional menin. (medlineplus.gov)

We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. (rupress.org)

The diagnosis of diffuse large cell lymphoma is usually confirmed after positive findings are obtained from a lymph node biopsy specimen. (medscape.com)

The present study was performed to investigate the effect of TTF1 expression in tumor cells on locoregional recurrence-free survival (LRFS), metastasis-free survival (MFS), and overall survival (OS) in patients irradiated for limited disease SCLC (LD-SCLC). (iiarjournals.org)

The NFAT family transcription factors play crucial roles in immunological and other biological activities. (aai.org)
An exciting finding is that in approximately half of the cases, there are recurrent chromosomal rearrangements resulting in the fusion of transmembrane protease serine 2 (TMPRSS2 ) and a member of the ETS family transcription factors , most commonly ERG . (medscape.com)

In NSCLC, the expression of TTF1 has been shown to inhibit cell migration and invasion ( 7 ). (iiarjournals.org)

To examine the relationship between epidermal cell development and cuticle assembly in the context of fruit surface, we investigated the tomato (Solanum lycopersicum) MIXTA-like gene. (fmach.it)
The understanding of epidermal cell determination is greatest for trichomes (hairs) in Arabidopsis . (biologists.com)
These consist of a single epidermal cell which branches towards its apex. (biologists.com)

Anatomy18

Organisms8

Chemicals and Drugs136

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes70

Disciplines and Occupations1

Information Science3

Proteins19

Differentiation30

Genes19

Proliferation12

Protein23

Gene expression9

Induces4

Basic Helix-Loop-Helix Transcrip1

Nuclear8

Regulates3

Regulate4

Abstract1

Epithelial9

Transcriptional8

Tumor cell4

Promoter5

Differentially expressed1

Somatic cells2

Neuronal1

Overexpression4

Biology4

Regulatory4

Lineages2

Nucleus3

Vitro4

Reverse transcri2

Chromatin2

Quantitative2

Apoptosis5

Hematopoietic5

Conical cell1

Cancer15

Enhancer2

Diffuse large B-1

Developmental1

Mesenchymal Stem1

Metabolism1

Progenitor cell1

Human Embryonic Stem1

Regulation1

Regulator1

MRNA2

Expression of transcription factors1

Ubiquitin1

Molecular2

Mice3

Stem cells2

Embryonic3

Leucine zipper2

Biol3

Downregulation4

FOXP31

Encodes2

Molecules2

Cellular3

Functional2

Inhibition1

Lymphoma1

Metastasis1

Family transcription factors2

Migration and invasion1

Epidermal cell3