Cell tracking experiments. Medical search. Frequent questions

Airway epithelial cell migration and proliferation capacities on normal and stiff substrates were evaluated through video-microscopy and flow cytometry. (frontiersin.org)
The results showed that migration and proliferation of ITGB4 deficiency cells were noticeably inhibited, along decreased cytoskeleton stabilization, and hampered RhoA activity, especially for cells cultured on the stiff substrate. (frontiersin.org)
The G.I. track undergoes cellular maturation or proliferation of epithelial cells during development, a process that is tightly repressed by the transcriptional repressor protein NCoRI. (nih.gov)
They will use a combination of zebrafish and cell culture models to decipher how Wnt signals are transduced through the cell, and how these signaling events lead to hematopoietic stem cell identity and proliferation. (nih.gov)
ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments for fixable fluorescent staining. (biotium.com)
Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. (biotium.com)
Combining mathematical modelling with organ-wide, multispectral FRaeppli-NLS lineage tracing in zebrafish, we demonstrate that a precise BEC-to-hepatocyte ratio is established (i) fast, (ii) solely by heterogeneous lineage decisions from uni- and bipotent progenitors, and (iii) independent of subsequent cell type-specific proliferation. (bvsalud.org)

When NCoRI is selectively eliminated in the G.I. track by targeted knockout of the gene, the intestinal track accelerates epithelial cell maturation, resulting in large genomic changes. (nih.gov)
A Cdk5-deficient human corneal limbal epithelial cell line was generated by lentiviral transduction of small hairpin RNA specific for Cdk5 (shCdk5-HCLE cells). (molvis.org)
Examining the effect of Cdk5 on E-cadherin containing epithelial cell-cell adhesions using a corneal epithelial cell line (HCLE), we found that Cdk5 and Cdk5 (pY15) coimmunoprecipitate with E-cadherin and Cdk5 (pY15) colocalizes with E-cadherin at cell-cell junctions. (molvis.org)
Rank signaling pathway regulates mammary gland homeostasis and epithelial cell differentiation. (bvsalud.org)

progenitor cells are harvested from the patient's healthy muscle tissue sample, cultivated in vitro and then grafted onto the patient's damaged tissues. (phys.org)
We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. (nih.gov)
Yet, current in vitro experiments are not always relevant to complex and variable cell conditions. (universiteitleiden.nl)
The lab is primarily interested in the Wnt signaling mechanisms governing how blood stem cells are made and maintained, with the ultimate goals of targeted cancer therapeutics and deriving blood stem cells in vitro. (nih.gov)
Taken together, these studies demonstrated gRNAs designed to highly conserved functional regions have near 100% efficacy in vitro in cells known to have received the Cas9/gRNA pair. (jefferson.edu)
We observed that NEU3-treated cells had suppressed LFA-1 adhesion to an ICAM-1 coated surface using an in vitro static adhesion assay. (frontiersin.org)

Led by scientists at the State University of New York-Stony Brook and Cold Spring Harbor Laboratory, the research team first analyzed different types of brain cells extracted from both embryonic and adult mice. (nih.gov)
In accordance with our previous studies regarding the functions of ITGB4 in bronchial epithelial cells (BECs), we hypothesize that the decreased ITGB4 expression during embryonic stage leads to abnormal ECM remodeling and increased tissue stiffness, thus impairing BECs motility and compromising lung development. (frontiersin.org)
The fact that humans and other living organisms can develop and grow from a single cell relies on a process called embryonic development. (sciencedaily.com)
C. elegans is an excellent model organism in which to study embryonic development due to its well-defined stereotypic cell lineage and powerful genetic tools. (nih.gov)
The submitting entity should have the authority and/or rights pertaining to the human embryonic stem cell line (e.g., be the owner, deriver or licensee or have written permission of the same to submit). (nih.gov)
The NIH has developed the NIH Human Embryonic Stem Cell Registry . (nih.gov)
Please check the current NIH Human Embryonic Stem Cell Registry page for the most up-to-date list. (nih.gov)
To obtain these cells, please follow the "see details" link, which is found under the Cell Line name, within the NIH Human Embryonic Stem Cell Registry . (nih.gov)
The stem cell lines remain the property of the individual stem cell providers, as listed on the NIH Human Embryonic Stem Cell Registry. (nih.gov)
Section I states that "These Guidelines apply to the expenditure of National Institutes of Health (NIH) funds for research using human embryonic stem cells (hESCs) and certain uses of induced pluripotent stem cells. (nih.gov)
Sections II and III apply specifically to human embryonic stem cells (hESCs). (nih.gov)
The prohibitions detailed in Section IV apply to all types of human pluripotent stem cells, including, but not limited to, those developed by methods such as the expression of genes involved in establishing pluripotency (e.g., the "Yamanaka factors") and the culturing of embryonic germ cells from primordial germ cells. (nih.gov)
By making a few simple genetic tweaks, scientists can transform mouse skin cells directly into brain cells, without first returning them to the embryonic state required by previous methods. (technologyreview.com)
And scientists say the technique is faster than the existing approach to generating replacement brain cells from an individual patient: reprogramming adult cells to behave like embryonic cells and then prodding them to develop into neurons. (technologyreview.com)
embryonic stem cell-like cells that can be made from virtually any cell in the body, thus avoiding the controversy involved in using stem cells derived from embryos. (scienceblog.com)
Dave Buchholz, first author of the article in Stem Cells, explained that by using induced stem cells that can be derived from patients, the scientists avoid immune rejection that might occur when using embryonic stem cells. (scienceblog.com)
Extending lineage tracing to adulthood determined that embryonic cells undergo spatially heterogeneous three-dimensional growth associated with distinct environments. (bvsalud.org)

Extracellular matrix that provides the microenvironment for progenitor cells in vivo contains hundreds of various proteins, lipids, and carbohydrates, which play a crucial role in tissue regeneration. (phys.org)
Longkun Li et al, Biofabrication of tissue-specific extracellular matrix proteins to enhance the expansion and differentiation of skeletal muscle progenitor cells, Applied Physics Reviews (2019). (phys.org)
they help prepare the cell for cell division and migration and work as a railway track on which motor proteins transport materials within the cell. (phys.org)
The gene regulatory networks that control gene expression are usually composed by several thousands of genes which are transcribed and translated to produce proteins that have a function in the cell. (hindawi.com)
Imagine the possibilities-drugs, lipids, metabolites, proteins, transcripts, genes, and metals can now be precisely studied within single cells, making it an indispensable asset in the realm of drug discovery. (yokogawa.com)
We can also further engineer these cells, enabling them to produce enzymes, antibodies, and other proteins for additional therapeutic benefit. (workopolis.com)
ViaFluor® SE dyes are membrane-permeant compounds that are initially non-fluorescent esters, but are converted to fluorescent dyes by intracellular esterases and will covalently react with amine groups on intracellular proteins at the same time, forming fluorescent conjugates that are retained in the cell. (biotium.com)
They also conducted experiments using harmless, hollow, virus-like particles coated with the critical envelope proteins that activate the PI3 kinase pathway. (scienceblog.com)
Total internal reflection fluorescence (TIRF) microscopy was performed on cells transfected with green fluorescent protein (GFP)-E-cadherin or GFP-p120, and internalization of boundary-localized proteins was analyzed with particle tracking software. (molvis.org)
Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. (intechopen.com)
Investigating several of the knockout cell lines in detail, the researchers found that the proteins produced by the impaired genes were truncated but retained some functionality. (genomeweb.com)
The researchers followed this experiment with a mass spec analysis using TMT isobaric labeling to quantify levels of proteins produced by the deletion-carrying genes, finding that roughly one-third of the knockout lines showed residual protein expression. (genomeweb.com)

Scientists have been actively pursuing the study of these cells, known as neural progenitor cells, since they were first spotted in the adult human brain several years ago. (nih.gov)
Earlier studies suggested that the development of new neurons from progenitor cells, called neurogenesis, is disrupted in a wide range of disorders. (nih.gov)
As reported in the November 9, 2007, issue of Science , researchers have developed a technique that uses magnetic resonance spectroscopy (MRS) to detect molecules within the brain that seem to be unique to neural progenitor cells. (nih.gov)
They found that neural progenitor cells had a specific chemical signal, or "biomarker," that was less common in the other types of brain cells. (nih.gov)
Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenitor cell (SMPC) functions that are essential for myoregeneration. (nature.com)
Scientists from Sechenov University, conjointly with their fellow Chinese and American researchers, have examined the latest advances in the use of skeletal muscle progenitor cells, specifying the core challenges inherent to the applicability of MPCs in cell therapy, and outlining the most promising breakthrough technologies. (phys.org)
Progenitor cells are cells that have the capacity to evolve (or differentiate) into a specific type of cell, for instance, muscle tissue cells. (phys.org)
The method requires the appropriate environment (similar to that in the human body ) to enable the differentiation of progenitor cells under laboratory conditions. (phys.org)
However, being highly sensitive to the subtlest changes in the growth-supporting microenvironment, progenitor cells may alter their behavioural patterns ex vivo and lose the ability to differentiate into target types of cells. (phys.org)
The research demonstrates that proper management of progenitor cell behaviour requires both a suitable scaffold (or a 'backbone' on which the tissue is cultivated) and extracellular matrix that interconnects the surrounding cells and regulates the intracellular processes. (phys.org)
The persistence of immune deficiency throughout life suggests that the cellular target of AHR activation is a fetal hematopoietic progenitor or stem cell. (nih.gov)
We next analyzed the fetal hematopoietic progenitor cells for changes in reactive oxygen species (ROS). (nih.gov)
Endothelial progenitor cells (EPCs), showing active migration and incorporation into neovasculature of glioma, may be a good vehicle for delivering genes to target GSCs transdifferentiation. (oncotarget.com)
In the liver, uni- and bipotent progenitor differentiation into hepatocytes and biliary epithelial cells (BECs), and their relative proportions, comprise the functional architecture. (bvsalud.org)
Our findings uncover heterogeneous progenitor contributions to tissue architecture-defining cell type proportions and postembryonic organ growth as key mechanisms forming the adult liver. (bvsalud.org)

7. A robust algorithm for segmenting and tracking clustered cells in time-lapse fluorescent microscopy. (nih.gov)
Scientists used a novel live-cell fluorescent imaging system that allowed them for the first time to identify individual viral particles associated with HIV infection. (sciencedaily.com)
ABOVE: A fluorescent PIN auxin transporter, PIN2 (magenta), appears only in the top part of plant cells. (the-scientist.com)
The team used a line of transgenic Arabidopsis plants to track fluorescent PIN auxin transporters-which influence plant cells' sense of direction-and found that mother cells communicated the direction of up and down to their daughter cells, but exactly how is unclear. (the-scientist.com)
Organic materials, such as fluorescent dyes, are commonly used as tracers for water leaks and tracking cells in biological experiments. (pnnl.gov)
With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining. (biotium.com)
They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. (intechopen.com)
Concomitant advances in fluorescence microscopy and fluorescent probes have allowed researchers to observe transcription events at single molecule level in single live cells at high spatial resolution and at different orders of temporal scales [1]. (nih.gov)
and (ii) reusability - most of the modules can be used for quantitative analysis of single cell and/or single molecule fluorescent imaging. (nih.gov)
For imaging with an automated screening microscope, cells were visualised using H-33342 (intercalates into DNA) and calcein-AM (fluorescent in viable cells only). (nih.gov)

Flow cytometry is a technique for counting and examining microscopic particles, such as cells and beads, by suspending them in a stream of fluid and passing them by a detection apparatus. (nih.gov)
A machine learning analysis was performed on a dataset acquired using flow cytometry from rodent heart cells treated with doxorubicin (DOX) and poly lactic-co-glycolic acid (PLGA). (pointloma.edu)
Fixable cytoplasmic stains for monitoring cell division by flow cytometry. (biotium.com)
The number of magnetically labeled EPCs estimated from T 2 maps correlated well with direct measurements of labeled cell counts by flow cytometry. (oncotarget.com)

Researchers found a unique population of stem cells in the roof of the mouth that quickly respond to stress from chewing and injury. (nih.gov)
Researchers have been studying oral stem cells to try to understand how the mouth rapidly recovers from injury, even in the face of constant challenges from chewing and exposure to microbes. (nih.gov)
Back in 2005, Norwegian researchers discovered 'grid cells' - special brain cells that track location just like a GPS, helping the brain maintain an internal map of its immediate environment. (kingworldnews.com)
The National Institutes of Health has named two biological engineering researchers as winners in Phase 2 of its Follow that Cell Challenge. (nih.gov)
The researchers conducted an array of experiments to show that the D2 region preferentially attaches to expanded microtubules. (phys.org)
P lant cells inherit their sense of up and down from their mother cells, researchers reported December 3 in Nature Plants . (the-scientist.com)
The Brown researchers showed in 2021 how their approach can profile the topological traits of one cell type that organizes into different spatial configurations and could make predictions on it. (sciencedaily.com)
This drives down the computation time from hours to seconds, enabling the researchers to compare thousands of simulations of cell organization by using the fingerprints to classify them into similar patterns without human input. (sciencedaily.com)
The researchers say the goal is to work backward and infer the rules that describe how different cell types arrange themselves based on the final pattern. (sciencedaily.com)
For instance, if they tinker with how certain cells are more adhesive or less adhesive, the researchers can identify how and when dramatic alterations occur in tissue architecture. (sciencedaily.com)
The husband and wife researchers are just starting their own prion experiments at the Broad Institute lab where they work. (bostonglobe.com)
University of Texas Medical Branch at Galveston researchers have discovered a key biochemical link in the process by which the Ebola Zaire virus infects cells - a critical step to finding a way to treat the deadly disease produced by the virus. (scienceblog.com)
Researchers may negotiate a material transfer agreement (MTA) with the cell providers in order to specify their rights and responsibilities concerning resulting data, publications, and potential patents. (nih.gov)
This question has captivated mitosis researchers for over half a century because the alignment of chromosomes and the formation of a metaphase plate is a universal feature of animal cells ( Pereira and Maiato, 2012 ). (biologists.com)
When the researchers used viruses to deliver two genes known to be particularly important for neural development into skin cells growing in a dish, they discovered that one of them had the power to convert the cells into what looked like immature neurons. (technologyreview.com)
The researchers are trying to repeat the process with human cells. (technologyreview.com)
To explore this question, the researchers used RNA sequencing to measure mRNA expression levels in 174 of the 193 knockout cell lines they produced, finding that the reduction in mRNA expression ranged widely across the different cell lines. (genomeweb.com)

6. TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies. (nih.gov)
10. An automatic method for robust and fast cell detection in bright field images from high-throughput microscopy. (nih.gov)
16. Image segmentation and dynamic lineage analysis in single-cell fluorescence microscopy. (nih.gov)
Traction force and Brillouin microscopy reveal asymmetries in force distributions and collagen stiffness during migration, providing evidence of mechanical cross-talk between cells and their substrate during migration. (nature.com)
TIRF microscopy of GFP-E-cadherin in transfected HCLE cells showed an actively internalized sub-population of E-cadherin, which was not bound to p120 as it was trafficked away from the cell-cell boundary. (molvis.org)

We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. (nature.com)
The interaction between extracellular matrix (ECM) and epithelial cells plays a key role in lung development. (frontiersin.org)
Cells sense a variety of extracellular cues, including the composition and geometry of the extracellular matrix, which is synthesized and remodeled by the cells themselves. (jove.com)

15. Automated tracking in live-cell time-lapse movies. (nih.gov)
Robust single particle tracking in live cell time-lapse sequences", Nature Methods, 5(8): 695-702, 2008. (nih.gov)

Moreover, the costameres at the cell surface interact with the repeating Z-disc component of the intracellular sarcomere, the contractile unit in muscle, through the actin cytoskeleton. (nature.com)
4. High-throughput detection and tracking of cells and intracellular spots in mother machine experiments. (nih.gov)
This type of intracellular comparison enables a substantially higher degree of quantitative analysis than is possible when comparisons must be made between different cells and essentially eliminates the effects of cellular response to ligand manipulation. (elifesciences.org)

Stem cells live in many types of adult tissues, including the lining inside the mouth, called the oral epithelium. (nih.gov)
The scientists labeled and tracked oral epithelial stem cells in mice and compared cell behaviors across different tissues in the mouth. (nih.gov)
Despite the existing multitude of artificial extracellular matrices, including those derived from animal tissues, native human tissues remain the most favourable environment for cell cultivation. (phys.org)
The research community needs tools that can probe living cells in real tissues to produce high dimensional time-series data from cells of interest. (nih.gov)
In tissues, there may be differences in how one cell adheres to the same cell type, relative to how it adheres to a different cell type," said Ian Y. Wong, an associate professor in Brown's School of Engineering who helped develop the algorithm. (sciencedaily.com)
For staining of living tissues, the specimen would need to be thin enough to allow exposure of the cells to the 36 kDa Annexin V protein. (biotium.com)
Also, damage to cell membranes from dissection or sectioning of tissues could result in high background staining. (biotium.com)
Section IV pertains to "hESCs and human induced pluripotent stem cells, i.e., human cells that are capable of dividing without differentiating for a prolonged period in culture, and are known to develop into cells and tissues of the three primary germ layers. (nih.gov)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Emerging evidence suggests that glioma stem-like cells (GSCs) transdifferentiating into vascular endothelial cells (ECs) possibly contributes to tumor resistance to antiangiogenic therapy. (oncotarget.com)
Along each step of this process different cell adhesion molecules and their ligands mediate recognition between leukocytes and endothelial cells. (frontiersin.org)
The Physiology Unit studies the mechanisms by which endothelial alpha globin regulates signaling between endothelial cells and vascular smooth muscle cells in human resistance arteries. (nih.gov)

This microenvironment is extremely active and its internal processes are essential for cell growth and migration. (phys.org)
Tangential neuronal migration occurs along different axes from the axis demarcated by radial glia and it is thought to have evolved as a mechanism to increase the diversity of cell types in brain areas, which in turn resulted in increased complexity of functional networks. (springer.com)
Growing evidence suggests that the physical properties of the cellular microenvironment influence cell migration. (nature.com)
However, it is not currently understood how active physical remodelling by cells affects migration dynamics. (nature.com)
Here we report that cell clusters seeded on deformable collagen-I networks display persistent collective migration despite not showing any apparent intrinsic polarity. (nature.com)
Combining theory and experiments, we show that crosslinking collagen networks or reducing cell cluster size results in reduced network deformation, shorter viscoelastic relaxation time and smaller gradients, leading to lower migration persistence. (nature.com)
Additionally, magnetic resonance imaging could track the migration and incorporation of EPCs into glioma in vivo , which was confirmed by Prussian blue staining. (oncotarget.com)
We addressed these questions for the neural crest cell migration test (cMINC). (nih.gov)

Mammalian cell biology is dynamic, but today's 'omic approaches require destruction of cells to access their molecular contents and cannot resolve the full richness of cellular diversity in situ . (nih.gov)
This approach -- and the ability to say 'that virion infected that cell' -- will help bring clarity to the field," said principal investigator Thomas Hope, a professor of cell and molecular biology at Northwestern University Feinberg School of Medicine. (sciencedaily.com)
The laboratory of Dr. Stephanie Grainger in the Department of Cell Biology in the Van Andel Institute (VAI). (nih.gov)
Dr. Grainger's research focuses on the molecular mechanisms underlying Wnt signaling, hematopoietic stem cell biology and cancer. (nih.gov)
The department provides a stimulating multidisciplinary environment for state-of-the-art research to understand the intricate mechanics of cell biology and their implications for human health. (nih.gov)
A Ph.D. graduate with experience in the fields of cell biology, developmental biology and/or zebrafish research (or equivalent). (nih.gov)
Experience in some of the following skills: zebrafish work, cell culture (pluripotent stem cells would be an asset), molecular biology techniques, stem cell biology. (nih.gov)
All of which makes understanding how different cell types organize into a complex tissue architecture one of the most fundamental questions in developmental biology. (sciencedaily.com)
To address these questions, we will combine the strengths of classical C. elegans forward genetics with CRISPR-Cas-9-mediated genome editing, next-generation sequencing, cell biology, and biochemical techniques. (nih.gov)
The convergence of cell biology and genetic engineering is creating fundamental new ways to impact disease. (workopolis.com)
As part of BlueRock's vision to change the future of medicine by enabling ground-breaking cell therapies, we're looking for a QC Analytical Scientist II, specialized in Cell Biology to support a dynamic and progressive analytical team. (workopolis.com)
Although much work remains to be done, we believe our results underscore the potential for stem-cell based therapies in the treatment of age-related macular degeneration," said Sherry Hikita, an author on both papers and director of UCSB's Laboratory for Stem Cell Biology. (scienceblog.com)
The research is the result of a collaboration between research groups led by Clegg, professor in the Department of Molecular, Cellular & Developmental Biology and co-director of UCSB's Center for Stem Cell Biology and Engineering, and Pete Coffey, professor of Cellular Therapy and Visual Sciences at University College London and director of the London Project to Cure Blindness. (scienceblog.com)

CAMSAP3 plays a role in various cellular phenomena, such as cell-cell binding and the development of neurons and cancer cells, through its microtubule-stabilizing ability," says Shima. (phys.org)
Ligand binding is followed by activation of elaborate signal transduction pathways in the cell, which ultimately mediate cellular decision-making. (elifesciences.org)
This groundbreaking tool enables the sampling of single living cells and their sub-cellular compartments under the keen observation of a confocal microscope. (yokogawa.com)
Prepare to be amazed by groundbreaking experiments showcasing the measurement of anti-TB drugs in single cells, tracking changes in single cell lipid profiles in response to chemotherapeutic drugs, and the extraction of individual nuclei and other sub-cellular compartments. (yokogawa.com)
BlueRock Therapeutics, a wholly-owned and independently operated subsidiary of Bayer AG, is a leading engineered cell therapy company using its unique cell+gene platform to direct cellular differentiation and genetically engineer cells to create a new generation of cellular medicines that address large patient populations suffering from neurological, cardiovascular, and immunology diseases. (workopolis.com)
Our foundational science harnesses the ability to create and then direct the differentiation of universal pluripotent stem cells into authentic, functional cells that can be used as allogeneic cellular therapies to treat a broad array of diseases. (workopolis.com)
Champion assessing and implementing new technologies, processes, and methods critical to safety and quality of cell seeds, banks, intermediates, drug substance and drug products for cellular therapeutics and other materials. (workopolis.com)
The UTMB group tied Ebola's cellular invasion mechanism to a series of biochemical reactions called the phophoinositide-3 kinase pathway (named for an enzyme found in the cell membrane). (scienceblog.com)

Section IV details types of human pluripotent stem cell research that are not eligible for NIH funding, even when using hESCs that meet the Section II eligibility criteria. (nih.gov)
Pluripotent means that the cells can become almost any cell in the body. (scienceblog.com)
The hope is that by transplanting fresh RPE, derived from induced pluripotent stem cells, the photoreceptors will stay healthy, preventing vision loss. (scienceblog.com)

Cell Biol. (nature.com)

Scientists have developed the first noninvasive technique for detecting cells in the living human brain that give birth to new neurons and other types of brain cells. (nih.gov)
As in the rodent experiments, the scientists found higher levels of the biomarker in the human brain's hippocampus than in the cortex. (nih.gov)
Next, the scientists examined the stem cells' reactions to different levels of physical stress. (nih.gov)
By analyzing the cells' RNA sequences, the scientists identified a gene, Lrig1 , which showed high activity, or expression, in the slow-cycling stem cells. (nih.gov)
The scientists have designed and tested a tissue decellularisation method that efficiently removes the cell components, while preserving its structural support-the matrix-and active compounds (cytokines, growth factors), which essentially control the cell behaviour. (phys.org)
Northwestern Medicine scientists have developed a novel method of tracking HIV infection, allowing the behavior of individual virions -- infectious particles -- to be connected to infectivity. (sciencedaily.com)
In the 1970s, scientists discovered that cells within frog embryos could be gently separated apart and when they were mixed back together, they would spontaneously rearrange into their initial organization. (sciencedaily.com)
The tracers are rugged enough to be deployed in harsh environments to track mass and improve scientists' understanding of environmental fate and transport. (pnnl.gov)
Scientists say the DNA changes detected in the study will prove much more accurate than existing biomarkers such as the prostate-specific antigen (PSA) associated with prostate cancer, which may yield false positives because even healthy cells can produce the protein. (technologyreview.com)
Santa Barbara, Calif.) - - An international team of scientists has rescued visual function in laboratory rats with eye disease by using cells similar to stem cells. (scienceblog.com)
The scientists worked with rats that have a mutation which causes a defect in retinal pigmented epithelial (RPE) cells and leads to photoreceptor death and subsequent blindness. (scienceblog.com)

Fluorescence Methods for Investigation of Living Cells and Microo. (intechopen.com)

1. DeLTA: Automated cell segmentation, tracking, and lineage reconstruction using deep learning. (nih.gov)
9. SuperSegger: robust image segmentation, analysis and lineage tracking of bacterial cells. (nih.gov)
19. ESC-Track: A computer workflow for 4-D segmentation, tracking, lineage tracing and dynamic context analysis of ESCs. (nih.gov)
Although Rank receptor is expressed by basal cells and luminal progenitors, its role in each individual cell lineage remains unclear. (bvsalud.org)

It also showed that cell viability was closely correlated to DOX concentration and cell autofluorescence. (pointloma.edu)
The apoptosis, necrosis and cell viability assays are designed to stain dissociated cells in culture and have not been validated for organ culture. (biotium.com)
Can I use the MTT or XTT cell viability kit if my cells have more than 10% FBS? (biotium.com)
the cell viability was determined from images of the well periphery. (nih.gov)

The brain's navigation system - which keeps track of where we are in space - also monitors the movements of others, experiments in bats and rats suggest. (weizmann-usa.org)
In experiments spearheaded by UCL's Amanda Carr, the team found that by surgically inserting stem cell-derived RPE into the retinas of the rats before photoreceptor degeneration, vision was retained. (scienceblog.com)
They found that the rats receiving the transplant tracked their visual focus in the direction of moving patterns more efficiently than control groups that did not receive a transplant. (scienceblog.com)

It is shown that in comparison with organic dyes, quantum dots have superior photostability of tracking apoptosis in cancer cells for longer time. (intechopen.com)

Furthermore, developmental AHR activation by TCDD increased ROS in the fetal hematopoietic stem cells, and the elevated ROS was associated with a reduced capacity of the TCDD-exposed fetal cells to compete with control cells in a mixed competitive irradiation/reconstitution assay. (nih.gov)
Altogether, we expect that this approach will increase the throughput of gene expression dynamics experiments by a factor of at least hundred (100), thus opening the possibility of using this assay to screen hundreds of cells per experimental condition in a few hundred samples per experiment. (nih.gov)
b Graphical representation of the assay scheme: cells were seeded in 96-well plates containing silicone stoppers to create a cell-free circular area. (nih.gov)

5. 3DeeCellTracker, a deep learning-based pipeline for segmenting and tracking cells in 3D time lapse images. (nih.gov)

Illumina ChIP-Seq Input read mappings from the H1 Derived Mesenchymal Stem Cells, Library SK443, were processed into density graphs of raw signal representing the aligned read density. (genboree.org)
To better our understanding of the role of specific pro-resolving mediators (SPMs) in inflamed human bone marrow-derived mesenchymal stem cells (hBMMSCs), we studied the effects of Resolvin E1 (RvE1) and Maresin 1 (MaR1) in lipopoly-saccharide (LPS) stimulated hBMMSCs. (mdpi.com)

The winners will share $400,000 in prizes awarded for the development of new tools and methods for predicting the behavior and function of a single cell in complex tissue - and how that reflects the health of the tissue. (nih.gov)
Phase 1 of the Follow that Cell Challenge sought novel robust methods for analysis of individual cells to detect and assess changes in cell behavior and function over time either as a result of natural state changes or when perturbed. (nih.gov)
Now, in addition to single-cell sequencing technology, we have integrated methods to monitor temporal changes in the phosphorylation and activation of transcription factors. (nih.gov)
In part, the uncertainty persisted because previous methods in HIV research have been unable to distinguish between viral particles that actually lead to infection of the cell, and those that are irrelevant. (sciencedaily.com)
Liaise with project teams to establish and perform test transfer of scientifically sound QC test methods to be used for the release and/or characterization of cell therapies. (workopolis.com)
Ensure that experiments, analytical methods and data meet the requirements for the intended use of assays, which includes lot release, comparability and/or characterization. (workopolis.com)

2) Developing a new generic method for the controlled delivery of any (bio)molecule directly into the cytoplasm of a cell thereby omitting endocytotic pathways. (universiteitleiden.nl)
During the course of infection, HIV fuses onto a target immune cell and delivers its capsid -- a cone that holds the genetic material of the virus -- into the cell's cytoplasm. (sciencedaily.com)
They demonstrated that uncoating leading to infection occurs early in the cytoplasm, around 30 minutes after cell fusion. (sciencedaily.com)

It was also more common in the mouse hippocampus, a brain region where neurogenesis occurs constantly, than in cells from the brain's cortex, where new neurons do not normally arise. (nih.gov)
The brain, composed of 85 billion neurons and trillions of connections with an equal number of glial, vascular and immune cells, functions as an interrelated web to process information. (nih.gov)
A cocktail of three genes can transform skin cells into neurons (shown here in red). (technologyreview.com)
The resulting cells express neuron-specific genes, have the characteristic branching shape of neurons, and form connections both with each other and with regular neurons collected from the brain. (technologyreview.com)

The intent of this initiative is to facilitate the use of stem cell-based therapies for regenerative medicine. (nih.gov)
The research shows the potential for stem cell-based therapies to treat age-related macular degeneration in humans. (scienceblog.com)

Contracting B-cells generate centripetally moving actin foci from lamellipodial F-actin networks in the B-cell plasma membrane region contacting antigen-presenting surfaces. (elifesciences.org)
The experiments also showed how wrongly placed transporters were destroyed and new ones were generated and inserted into the correct part of the cell membrane. (the-scientist.com)
Clustering of ligand:receptor complexes on the cell membrane is widely presumed to have functional consequences for subsequent signal transduction. (elifesciences.org)
Within the plasma membrane environment, glycoconjugate-receptor interactions play an important role in the regulation of cell-cell interactions. (frontiersin.org)
Cell surface glycoconjugates are critical components of the plasma membrane. (frontiersin.org)

The natural environment presents a continuous stream of sensory stimuli that animals must quickly evaluate to choose an appropriate behavioral response - the laboratory aims to understand the functional development of neuronal connections which mediate these choices To achieve this, the laboratory combines computational analysis of behavior with classical genetic and cell-based circuit manipulations in zebrafish. (nih.gov)
To fully harness the power of zebrafish for dissecting neuronal circuits, the laboratory has developed software and transgenic tools to advance neurobiological experiments in zebrafish. (nih.gov)

[ 33 ] They used a transgenic mouse that expressed Bclxl in T cells in a constitutive fashion. (medscape.com)

Like poles support a tent, microtubules-hollow cylindrical structures made of tubulin protein-support eukaryotic cells. (phys.org)
We believe that an approach, such as the one shown here, could help to understand the one regulation of transcription in eukaryotic cells. (hindawi.com)
The discovery of general patterns of cooperativity and coregulation for the transcriptional regulation program of eukaryotic cells will help to understand the control of genome-wide expression and how it influences the establishment of phenotypes. (hindawi.com)

Keratinocytes, the predominant cell type of the epidermis, migrate to reinstate the epithelial barrier during wound healing. (nih.gov)
Fig. 1: Cell clusters migrate persistently on collagen networks. (nature.com)
After 1 day, the stoppers were removed, and cells were allowed to migrate for 24 h before the test compounds were added for additional 24 h. (nih.gov)

Antigen-induced B-cell receptor (BCR) signaling is critical for initiating and regulating B-cell activation. (elifesciences.org)
This is an important study highlighting a distinct role of WASP dependent actin foci in B cell antigen receptor signalling. (elifesciences.org)
The proposal of higher molecular density in B cell receptor clustering leading to kinase exclusion and attenuated signalling is provocative as it contrasts with models for other antigen receptors. (elifesciences.org)
Antigen-induced receptor reorganization activates BCRs at the B-cell surface. (elifesciences.org)
Early reports dubbed increases in B cell antigen sensitivity a "neuraminidase effect," ( Cowing and Chapdelaine, 1983 ) and recent evidence has ascribed this phenomenon to sialic acid acting as a negative regulator of immune cell interactions ( Bagriacik and Miller, 1999 ). (frontiersin.org)

Reporting to the Senior Analytical Scientist of Quality Control, the Analytical Scientist II will be a key technical contributor of an analytical team establishing the QC testing methodology of cell-based therapeutics destined for human clinical trials, from starting stem cell materials to final differentiated cell therapy. (workopolis.com)

In this case, they utilized the approach to monitor how the HIV capsid uncoats in the cell at the individual particle level. (sciencedaily.com)
By activating the PI3 kinase pathway, they found, an Ebola virus particle tricks the cell into drawing it into a bubble-like compartment known as an endosome, which is pulled, together with the virus, into the cell. (scienceblog.com)
Cell aggregations were performed using HCLE, Cdk5 inhibitor olomoucine, ShCdk5, and MDA-MB 231 cells in the presence and absence of calcium, and particle size was measured using image analysis software. (molvis.org)

July 3, 2013 - See Companion PAR-13-252 Improvement of Animal Models for Stem Cell-Based Regenerative Medicine (R24). (nih.gov)

It has become routine to visualize the movement and progression of individual virions in cells, but the relevance of these observations was previously unclear, as many virions are defective or do not progress to make further copies of themselves. (sciencedaily.com)

This same group attempted to uncouple these 2 cell-death pathways to examine the effect of costimulatory blockade on tolerance induction. (medscape.com)
This allows us to monitor the activation of different transcription factors and the array of genes each one regulates to investigate how different signaling pathways interact in controlling gene expression in a single cell. (nih.gov)

Initial studies have shown selective upregulation of T-cell receptor Vbeta genes, raising the possibility of intragraft clonal expansion. (medscape.com)
For example, in the T cell receptor signaling system, linker for activation of T cells (LAT) phosphorylation results in the recruitment of the adaptor protein Grb2 through binding to multiple phosphorylated tyrosine sites on LAT. (elifesciences.org)

3. DeLTA 2.0: A deep learning pipeline for quantifying single-cell spatial and temporal dynamics. (nih.gov)
Studies in temporal urbanism: the urbanTick experiment. (wikipedia.org)
The data analysis pipelines used to extract this rich spatial and temporal information at single cell level generally require continuous and hands-on experimenter interaction with the workflow, thus limiting these approaches to a few tens of cells per sample, and to few samples per experiment. (nih.gov)

The absence of survival gene expression makes the cell sensitive to death secondary to cytokine withdrawal, which is also predisposed to by low levels of IL-2. (medscape.com)
Initially, we developed the technology to monitor changes in gene expression (genomics) in single cells. (nih.gov)

We will present a semi-automated high throughput analysis pipeline for studying transcription events (dynamics) in live eukaryotes cells. (nih.gov)

The team confirmed its findings by duplicating the experiment in elderly rhesus macaques, which, similar to older humans, also accumulate 4BL cells and develop insulin resistance. (nih.gov)

Expertise in performing and developing cell-based assays is required. (workopolis.com)
Collect and analyze data from various assays, track and trend data and support the establishment of specifications for critical quality attributes. (workopolis.com)
Transcriptomic analysis and functional assays point to the awakening of basal bipotency after pregnancy by the induction of Rank/NF-κB signaling in basal parous cell to restore lactation and tissue homeostasis. (bvsalud.org)

They also plan to transplant the reprogrammed mouse cells into the brains of engineered mice that have a disease similar to Parkinson's. (technologyreview.com)

In addition to providing specialized equipment, the RBC provides two fully equipped surgical suites, as well as tracking and programming tools for trial-by-trial analysis of behavior. (nih.gov)
The protocol can easily be adapted to study the contribution of almost any chemical component towards cell fate and cell behavior. (jove.com)

Cell death secondary to cytokine withdrawal was prevented, but cell death due to IL-2-mediated AICD (since the latter uses the fas-fas ligand pathway) occurred. (medscape.com)
Prepulse inhibition and other forms of startle modulation can be robustly induced in fish, where experiments can take advantage of the well described neuronal pathway for startle responses. (nih.gov)
In addition, he said, it was the first virus to be discovered interacting with the PI3 kinase pathway in order to enter cells, which could have profound implications. (scienceblog.com)

Overall, the results indicate that oral epithelial stem cells in the hard palate are sensitive to both genes and the environment, responding quickly to daily challenges such as eating and to higher-stress events like injury," Byrd says. (nih.gov)
Here, we built a transcription factor network for human monocytic THP-1 myeloid cells based on the experimentally curated FANTOM4 database where nodes are genes and the experimental interactions correspond to links. (hindawi.com)
On the other hand, a reduced set of genes, called transcription factors (TFs), has the role of regulating the transcriptional program of the cells. (hindawi.com)

Berninger B, Guillemot F, Götz M (2007) Directing neurotransmitter identity of neurones derived from expanded adult neural stem cells. (springer.com)

For example, nearly a third of human tumors undergo whole genome duplication [WGD] that can increase cancer cell fitness, but it is not known how or when these tetraploid cancers arise. (hopkinsmedicine.org)

Being able to connect infectivity of individual particles and how they behave in the cell to infection -- which is what we really care about -- is going to have a big impact on the field," Hope said. (sciencedaily.com)
P NNL's rugged tracer particles can be mass-manufactured with specific properties for tracking through harsh and extreme environments. (pnnl.gov)
Using a unique test created at UTMB that adds a light-emitting molecular beacon, called luciferase, to Ebola viruses and the virus-like particles, the investigators were able to determine exactly when and where each broke out of its bubble, and track its progress. (scienceblog.com)
A blasticidin-inducible vector for expression of Cdk5-specific short hairpin RNA (ShCdk5) was generated by recombination and packaged into non-replicative lentiviral particles for transduction of human corneal limbal epithelial (HCLE) cells. (molvis.org)

These results suggest that decreased ITGB4 expression results in increased lung tissue stiffness and impairs the adaptation of bronchial epithelial cells to substrate stiffness, which may be related to the occurrence of broncho pulmonary dysplasia. (frontiersin.org)

Furthermore, B-cell contraction increases BCR molecular density in individual clusters, leading to decreased BCR phosphorylation. (elifesciences.org)

New publications, experiments and breakthroughs in biomedicine-and what they mean. (technologyreview.com)

Our in vivo data also supported that ECM remodeling is important for SMPC engraftment and that fibrotic scars may represent one barrier to efficient cell therapy. (nature.com)

In order to engineer functional matrices, any cells and their components that may trigger immune reaction during grafting are mechanically isolated, or washed out with processing solution, from the target tissue sample. (phys.org)
The present study investigates the functional significance of Cdk5 in forming and maintaining cell-cell stability in corneal epithelial cells. (molvis.org)

To establish the utility of this multiplexed technology, we are employing it to study a critical biological question: How do cancer cells develop drug resistance? (nih.gov)
This observation, they noted, tracked with other recent research that found "high variation in NMD efficiency depending on a complex set of biological factors. (genomeweb.com)

This caused gut leakiness and a domino effect of inflammatory events that activated the immune system's white blood cells to convert another kind of immune cell, known as B1aB, into a potentially harmful type called 4BL cells. (nih.gov)
Sialic acid (also known as neuraminic acid, or Neu5Ac) has long been recognized to participate in the regulation of immune cell function. (frontiersin.org)

Inhibiting Cdk5 activity in HCLE or suppressing Cdk5 expression in a stable HCLE-derived cell line (ShHCLE) decreased calcium-dependent cell adhesion, promoted the cytoplasmic localization of E-cadherin, and accelerated the loss of surface-biotinylated E-cadherin. (molvis.org)
Cdk5 inhibition also reduced adhesion in a cadherin-deficient cell line (MDA-MB-231) expressing exogenous E-cadherin, although Cdk5 inhibition promoted adhesion when these cells were transfected with N-cadherin, as previous studies of Cdk5 and N-cadherin predicted. (molvis.org)
We have investigated the mechanism and activity of the human neuraminidase (NEU) isoenzyme, NEU3, on T cell adhesion receptors. (frontiersin.org)
Later steps of the process must arrest the cell (firm adhesion) to allow for transmigration. (frontiersin.org)
The prominent role of sialic acid in adhesion suggests that changes which affect sialoglycoconjugates (SGC) may be critical to regulation of cell-cell interactions. (frontiersin.org)

Some studies have used stably transduced cells under drug selection to accomplish this goal. (jefferson.edu)
The dyes also can be used to stably label cells to image cell morphology, or to track cell populations in mixed co-culture experiments. (biotium.com)

By understanding how alpha globin regulates cell-cell signaling in the vessel wall, we may be able to develop new strategies to modulate human endothelial function and treat or prevent diseases at the vascular endothelium. (nih.gov)

B-cell receptors (BCRs) expressed on the B-cell surface detect the presence of cognate antigens. (elifesciences.org)

The k-means clustering algorithm was used to identify three data clusters, then the random-forest classification algorithm was used to characterize the clusters as representing untreated cells, unviable DOX-treated cells, and viable DOX-treated cells. (pointloma.edu)
Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. (nature.com)

In the original murine model of infectious tolerance, [ 29 ] spleen cells from tolerant animals were able to induce tolerance in naive mice, and cells from the naive mice were able to induce tolerance in yet other mice. (medscape.com)
The NIA LMBI group had previously tracked how 4BL cells accumulated in elderly humans, primates, and mice. (nih.gov)
Further experiments showed that higher 4BL cell levels increased insulin resistance in aged mice. (nih.gov)
Feeding aged mice with Akk reduced inflammation, eliminating the 4BL cells and the related insulin resistance. (nih.gov)
In response to a small puncture wound in the hard palate of mice, the slow-cycling cells began rapidly dividing and migrated into the injury to renew the tissue. (nih.gov)
Feeding mice soft food (compared to a normal hard-pellet diet) evoked an opposite reaction: the stem cells divided less frequently. (nih.gov)
As further evidence, mice genetically engineered to lack Lrig1 had increased stem cell division. (nih.gov)

This FOA encourages Exploratory/Developmental Research grant (R21) applications from institutions and organizations proposing research aimed at characterizing animal stem cells and improving existing, and creating new, animal models for human disease conditions. (nih.gov)
While the proposed studies focus on the use of our nanopipette technology to study human cancer cells in culture, the cell monitoring capabilities of the nanopipette allow it to be used for in situ analysis of single cells in tissue biopsies and slices. (nih.gov)
I found that the same common mutations are associated with WGD in human lung adenocarcinomas and demonstrated that these oncogenes can cause WGD in primary human airway cells. (hopkinsmedicine.org)
What types of stem cell research are governed by the NIH Guidelines for Human Stem Cell Research? (nih.gov)
various free Complexity in Chemistry and Beyond: Interplay Theory and Experiment: New versus automation in the pulse of human ia in designers with student business or s individuals of fundamental time: a multi III mobile, Mechanical context. (4-buescher.de)
including for horizontal free Complexity in Chemistry and Beyond: Interplay Theory and Experiment: New and Old under medicine 8 of timbre 5, United States Code, of the large access seen by Secretary of Health and Human Services training to role with byF record results by part Assignments in focussing cells. (4-buescher.de)
We integrate human clinical investigations with experiments conducted in model systems. (nih.gov)

NCCs migrating into cell-free zones were imaged using a confocal microscope. (nih.gov)

Further study indicated that the biomarker probably corresponds to yet-unknown fatty molecules in the cells. (nih.gov)
However, tracking and manipulating single molecules inside a cell is still not possible. (universiteitleiden.nl)

Proliferation7

Epithelial cell4

Vitro6

Embryonic17

Proteins12

Progenitor15

Fluorescent10

Flow Cytometry4

Researchers17

Microscopy5

Extracellular matrix3

Live-cell time-lapse2

Intracellular3

Tissues9

Endothelial cells3

Migration8

Biology13

Cellular8

Pluripotent3

Biol1

Scientists11

Fluorescence1

Lineage4

Viability4

Rats3

Apoptosis1

Assay3

Time lapse images1

Mesenchymal Stem2

Methods6

Cytoplasm3

Neurons4

Stem cell-based therapies2

Membrane5

Zebrafish2

Transgenic1

Eukaryotic cells3

Migrate3

Antigen5

Therapeutics1

Particle3

Regenerative Medicine1

Unclear1

Pathways2

Receptor2

Temporal3

Gene expression2

Dynamics1

Confirmed its find1

Assays3

Transplant1

Behavior2

Pathway3

Genes3

Neural1

Genome1

Particles4

Substrate1

Molecular1

Breakthroughs1

Vivo1

Functional2

Biological2

Immune cell2

Adhesion5

Stably2

Regulates1

Receptors1

Clusters2

Mice7

Human7

Confocal1

Molecules2