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  • vitro
  • Moreover, the cytotoxicity and cellular uptake of compounds 5 - 9 have been analyzed in vitro at different concentrations of B (5, 50, and 100 μg B/mL) using HeLa cells. (icmab.es)
  • The exceptional cellular uptake and intracellular boron release, together with the fluorescent and biocompatibility properties, make compounds 7 and 8 good candidates for in vitro cell tracking. (icmab.es)
  • Such dynamically tunable hydrogels can be utilized as in vitro cellular micro-environments that can exert time-dependent stimuli to mechanically regulate target cells. (rsc.org)
  • We analyzed viability and functionality of stained cells in vitro and investigated their ability to migrate to sites of inflammation in vivo in a dermatitis mouse model via fluorescence reflectance imaging (FRI). (bmj.com)
  • people's
  • Washington, D.C.-The Electronic Frontier Foundation (EFF) urged the U.S. Supreme Court to review a troubling ruling that allows police to obtain-without a warrant-location data from people's cell phones to track them in real time. (eff.org)
  • Jersey and Delaware must show probable cause before tracking people's cell phone locations. (commondreams.org)
  • therapies
  • Cell-based therapies may gain future importance in defeating different kinds of diseases, including cancer, immunological disorders, neurodegenerative diseases, cardiac infarction and stroke. (springer.com)
  • person's
  • Today the Supreme Court announced it will review United States v. Carpenter , a case involving long-term, retrospective tracking of a person's movements using information generated by his cell phone. (eff.org)
  • Massachusetts police must now get a search warrant before they can track a person's past movements through their cell phone in an important new decision that has implications beyond just cell tracking in the Bay State. (eff.org)
  • The state appealed to the Massachusetts Supreme Judicial Court, and with the help of Kit Walsh of the Berkman Center for Internet and Society at Harvard Law School, we filed an amicus brief arguing that the trial judge got it right, and that a search warrant is needed before police can track a person's every movement for an extended period of time. (eff.org)
  • Particle
  • The labeling protocol is rapid, simple, and applicable to essentially any membrane-containing cell or particle ( Figure S2 *), but obtaining bright, uniform, and reproducible labeling requires attention to different variables than when staining with antibodies or other equilibrium binding reagents 1-3 . (sigmaaldrich.com)
  • heterogeneity
  • Due to genetic heterogeneity, some cells cross faster. (medicineatmichigan.org)
  • Most mitochondrial assays require either isolating the organelle, thus disrupting the intracellular signaling or using intact cells with average measurements for the entire population neglecting the ability to distinguish cell heterogeneity. (ahajournals.org)
  • warrant to track
  • The ruling came in a public records lawsuit brought by the American Civil Liberties Union, which had requested information on federal cases in which law enforcement had obtained cellphone tracking data without a warrant to track a user's whereabouts. (yahoo.com)
  • Thankfully, the high court agreed with us, becoming the second state supreme court-after New Jersey -to rule police need to get a warrant to track. (eff.org)
  • myeloid
  • Methods Immortalized murine myeloid progenitor ER-HoxB8 cells obtained from wildtype as well as S100A8/S100A9 -/- mice were differentiated to neutrophils or monocytes/macrophages 1 . (bmj.com)
  • zebrafish
  • Zebrafish have become a powerful vertebrate model organism for drug discovery, cancer and stem cell research. (spie.org)
  • The system is designed specifically for non-invasive tracking of both stationary and circulating cells in adult zebrafish casper, under physiological conditions in the same fish over time. (spie.org)
  • probable cause
  • Can the government use your cell phone records to track your physical location without first obtaining a warrant based on probable cause? (eff.org)
  • The Justice Department, in responding to the ACLU lawsuit, identified 229 prosecutions since 2001 in which a judge had approved the government's request to obtain cellphone tracking data without making a finding of probable cause. (yahoo.com)
  • NEW YORK - If you are a U.S. resident who owns a cell phone, you should care about the outcome of a court case that "could well decide whether the government can use your cell phone to track you - even if it hasn't shown probable cause to believe it will turn up evidence of a crime. (commondreams.org)
  • Civil liberties groups are asking the court to require that the government show probable cause before it can track your whereabouts. (commondreams.org)
  • Back in 2007, the U.S. government applied for court permission to obtain information about the location of an individual's cell phone, without showing probable cause that tracking the individual would turn up evidence of a crime. (commondreams.org)
  • A number of civil liberties groups, on behalf of plaintiffs in the case, filed a friend-of-the-court brief in support of the district court decision, arguing that district courts must require the government to show probable cause before permitting the government to obtain information about the location of a cell phone. (commondreams.org)
  • Imaging
  • Ahrens ET, Flores R, Xu H et al (2005) In vivo imaging platform for tracking immunotherapeutic cells. (springer.com)
  • Anderson SA, Shukaliak-Quandt J, Jordan EK et al (2004) Magnetic resonance imaging of labeled T-cells in a mouse model of multiple sclerosis. (springer.com)
  • Billotey C, Wilhelm C, Devaud M et al (2003) Cell internalization of anionic maghemite nanoparticles: quantitative effect on magnetic resonance imaging. (springer.com)
  • Cellular imaging using magnetodendrimers: application to human stem cells and neoplastic cells in vivo. (springer.com)
  • Longitudinal MR tracking of mesioangioblasts was achieved following SPIO labeling of cells followed by high-resolution imaging. (ismrm.org)
  • The study has determined GNTs are effective in helping doctors track stems cells through the body by making them 40 times better than standard contrast agents used in magnetic resonance imaging. (innovations-report.com)
  • Tests show GNTs are highly effective for tagging and tracking stem cells through magnetic resonance imaging. (innovations-report.com)
  • The confocal imaging part in this system serves the dual purposes of imaging fish tissue microstructure and a 3D navigation tool to locate a suitable vessel for circulating cell counting. (spie.org)
  • With the aid of stimulus-responsive hydrogel substrates composed of ABA triblock copolymer micelles, we monitored the morphological dynamics of myoblast (C2C12) cells in response to an abrupt change in the substrate elasticity by live cell imaging. (rsc.org)
  • bone marrow
  • Researchers at Rice and the Texas Heart Institute at St. Luke's Episcopal Hospital in Houston reported in the journal Biomaterials that mesenchymal stem cells drawn from pig bone marrow labeled with GNTs are easily spotted under MRI. (innovations-report.com)
  • simulators
  • From cell-site simulators in New York to facial recognition devices in San Diego , law enforcement surveillance technologies are spreading across the country like an infectious disease. (eff.org)
  • Illinois has joined the growing ranks of states limiting how police may use cell-site simulators , invasive technology devices that masquerade as cell phone towers and turn our mobile phones into surveillance devices. (eff.org)
  • The Desert Sun of Palm Springs made a similar request to the San Bernardino County Sheriff's Department, which said it had to maintain secrecy even though the newspaper found information online about cell site simulators. (washingtonexaminer.com)
  • Therapeutics
  • While we are still emerging from the foundational stages of this new field, we are already using engineered cells to discover underlying biological mechanisms, develop new therapeutics, and produce natural products. (harvard.edu)
  • viability
  • In our experience, a preliminary titration at constant cell concentration is the easiest way to select an optimized dye concentration that gives acceptable post-labeling viability, recovery, and fluorescence intensity as well as unaltered cell function 2,3 . (sigmaaldrich.com)
  • However, lack of physiologic salts can impair cell viability over prolonged periods, and the dye aggregates will gradually form even in the absence of salts 1-3 . (sigmaaldrich.com)
  • proliferation
  • The purpose of this pilot phase II trial is to identify the molecular and genetic mechanisms by which statins influence breast cancer cell proliferation. (bioportfolio.com)
  • mice
  • Multicolored intestine tissue in genetically modified mice allow scientists to track which cells give rise to tumors. (scitechdaily.com)
  • In their latest study , his team engineered mice to carry a gene for a drug-inducible marker that once activated caused labeled cells to make molecules that fluoresce one of four colors. (scitechdaily.com)
  • enforcement
  • Law enforcement officers in Washington, D.C. violated the Fourth Amendment when they used a cell site simulator to locate a suspect without a warrant, a D.C. appeals court ruled on Thursday. (eff.org)
  • That was the warning issued to the public by several major civil liberties organizations as they appeared in federal court in Philadelphia to argue for more privacy protections in the use of cell phones as tracking devices by law enforcement agents. (commondreams.org)
  • Before GPS started being deployed in the majority of mobile devices sold on the market, the only method available to track a cell phone was triangulating the cell phone's position using one of several techniques available to law enforcement and other governmental agencies. (tech-faq.com)
  • dyes
  • Ideal tracking dyes are passed to daughter cells for multiple generations but do not transfer to neighboring cells on contact. (thermofisher.com)
  • Invitrogen CellTracker dyes exhibit ideal properties-they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. (thermofisher.com)
  • Table S1 * summarizes non-perturbing staining conditions reported for several membrane dyes, but concentrations found in the literature should always be tested on each laboratory's cell type(s) of interest. (sigmaaldrich.com)
  • Diluent C is an aqueous staining vehicle designed to keep cells viable and membrane dyes in solution for long enough to complete the brief labeling step. (sigmaaldrich.com)
  • Cells were labeled with the membrane-selective fluorescent dyes DIR, DID and VivoTrack680 (IVT), respectively 2 . (bmj.com)
  • Results ER-HoxB8 cells could be effectively stained with all three fluorescent dyes. (bmj.com)
  • localization
  • The oldest and only clinically established method is leukocyte scintigraphy, which enables a (semi)quantitative assessment of cell assemblies and, thus, the localization of inflammation foci. (springer.com)
  • The critical role that acto-myosin complexes play in the morphological transition was verified by the treatment of cells with myosin II inhibitor (blebbistatin) and the fluorescence localization of focal adhesion contacts. (rsc.org)
  • tumors
  • The finding holds particularly interesting possibilities for malignant brain cancers whose cells aggressively spread throughout normal brain tissue seeding new tumors, a characteristic that makes treatment almost impossible. (genomenewsnetwork.org)
  • After two weeks, tumors that were treated with the therapeutic stem cells had shrunk by 80 percent. (genomenewsnetwork.org)
  • What underlies this scenario is that many tumors might be fueled by cancer stem cells, which produce other types of cancer cells, just as ordinary stem cells produce normal tissue. (scitechdaily.com)
  • They discovered that all tumors contain at least a few labeled cells, presumably the cancer's stem cells. (scitechdaily.com)
  • Tumors also contained many unlabeled cells, which could be killed off with standard chemotherapy, but the tumors quickly returned. (scitechdaily.com)
  • Once chemotherapy was paired with a genetic therapy to suppress the labeled cells, the tumors shrank back into residual vestiges. (scitechdaily.com)
  • Previously, Clevers and his team had shown that a genetic marker that labels healthy gut stem cells also labels stem cells in benign intestinal tumors, which are precursors of cancer. (scitechdaily.com)
  • As the tumors became more aggressive, they were more likely to produce more stem cells that can divide indefinitely and less likely to produce differentiated cells, which can only divide a limited number of times. (scitechdaily.com)
  • site simulator
  • In one of the rare court cases involving the device, the FBI acknowledged in 2011 that so-called cell site simulator technology affects innocent users in the area where it's operated, not just a suspect police are seeking. (washingtonexaminer.com)
  • methods
  • We analyze the results using performance measures for segmentation and tracking that rank all participating methods. (muni.cz)
  • visualize
  • However, in order to gain an acceptable signal-to-noise ratio, at a sufficiently high spatial resolution of the MR sequence to visualize a small amount of cells, experimental MR scanners working at high magnetic fields are usually required. (springer.com)
  • In this study, we use the dual-wavelength OR-PAM with CS to visualize T cell trafficking in a 3D culture system with higher temporal resolution. (spie.org)
  • processes
  • The simplest processes for monitoring cell movement and location use tracking probes that pass through the membrane into the cell and become membrane-impermeant after loading. (thermofisher.com)
  • This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism. (jove.com)
  • Cellular
  • This tracking has given us detailed information for the commonly used endpoints of 5, 7, and 14 days that is unclouded by cellular aggregation. (bioportfolio.com)
  • phone
  • Whenever you use a cell phone. (eff.org)
  • Police nationwide keep quiet about cell-tracking technology Police across the country may be intercepting phone calls or text messages to find suspects using a technology tool known as Stingray. (thenewsstar.com)
  • This Oct. 24, 2013 file photo shows a youth checking his smartphone in Glenview, Ill. Local police may be tracking your cell phone. (thenewsstar.com)
  • Noting that a cell phone is an "indispensable" part of modern life and are ubiquitous as almost a "permanent attachment" to our body, the court ruled that people have a right to be free from invasive, long term tracking of their movements. (eff.org)
  • While the state quibbled with whether cell tracking is as precise or accurate as GPS tracking of a car, the high court believed cell tracking implicated even greater privacy concerns than GPS tracking because unlike a device attached to a car, a cell phone is carried almost everywhere a person goes. (eff.org)
  • If the location information police can obtain from a cell phone wasn't precise, there'd be no reason for the government to request it. (eff.org)
  • Plus it noted users don't voluntarily turn their location information over to the cell phone provider in a meaningful way. (eff.org)
  • There's no question that cell phones and cell-phone records can be useful for police officers who need to track the movements of those they believe to be breaking the law," the group added. (commondreams.org)
  • The plaintiffs in the court case hope the court will "send a message that merely carrying a cell phone should not make people more susceptible to government surveillance. (commondreams.org)
  • Today, most cell phone tracking applications work by accessing the GPS location of the cell phone and providing feedback to the end-user in the form of an app or software program. (tech-faq.com)
  • In order to understand how cell phone tracking works, it becomes necessary to look at each major model of cell phone and the available options for locating the desired mobile phone. (tech-faq.com)
  • Some atmospheric facts can influence the accuracy of a GPS device, so when attempting to use a cell phone tracking application based on GPS, the locating data may be off as far as 50 feet from the exact location of the phone (a bigger deal for those who have misplaced a cell phone in their home as compared to trying to find out the geographic location of a phone). (tech-faq.com)
  • Before getting into detail on the various GPS tracking applications for cell phones, it's first worth reviewing some of the factors that can degrade the signal accuracy throwing off the location of the phone. (tech-faq.com)
  • progression
  • The role of tissue-resident memory T (T) cells in breast cancer progression has been difficult to study. (bioportfolio.com)
  • LGR5 acts as a target of miR-340-5p in the suppression of cell progression and drug resistance in breast cancer via Wnt/β-catenin pathway. (bioportfolio.com)
  • confocal microscopy
  • Cervical lymph nodes were then separately dissected as submandibular (SM) and superficial cervical (SC) lymph node (LN). Labeled DC were detected by confocal microscopy on LN sections or FACS analysis of LN cells at 24h and 48h after injection. (arvojournals.org)
  • towers
  • But even older phones that don't have chips can be tracked by knowing the location of the cell towers they use to connect to a network," the group said. (commondreams.org)
  • location
  • Often with EFF input as a friend of the court, the vast majority of judges issuing public opinions on the matter are saying "no", and rejecting government applications for cell site location tracking information made without showing sufficient need for this kind of sensitive information. (eff.org)
  • Cell movement and location studies require specialized probes that are nontoxic to living cells and are available in a range of fluorescent colors to match instrument lasers and filters and to accommodate co-staining with antibodies or other cell analysis probes. (thermofisher.com)
  • Longer-term tracking of cell movement and location requires probes that are more resistant to photobleaching and are retained through more cycles of cell division. (thermofisher.com)
  • membrane
  • U2-OS cells were transduced with CellLight Plasma Membrane-GFP (green), followed by labeling with Qtracker 655 Cell Labeling Kit (magenta), and HCS NuclearMask Blue (blue). (thermofisher.com)
  • The amount of dye required for bright, homogeneous labeling increases as total number and/or size of cells to be stained increases (i.e., as total amount of membrane increases). (sigmaaldrich.com)
  • It minimizes membrane dye aggregation and maximizes staining efficiency by avoiding salts, while minimizing cell toxicity by maintaining osmotic pressure and avoiding organic solvents. (sigmaaldrich.com)
  • Since labeling occurs by partitioning rather than equilibrium binding, dye uptake into membrane continues for as long as cells are in contact with dye, eventually compromising membrane integrity and function. (sigmaaldrich.com)
  • Changes in membrane potential, oxidative stress and intracellular calcium were simultaneously assessed for every single cell during these time points. (ahajournals.org)
  • tissue
  • Ultimately, the team hopes the magnetic properties of tagged stem cells will allow doctors to manipulate them in vivo and direct cells to specific locations -- in the heart, for instance -- where they can heal damaged tissue. (innovations-report.com)
  • assay
  • However, cellassociated dye aggregates are not efficiently removed by washing and act as reservoirs of unbound dye that can transfer to unlabeled cells present in an assay. (sigmaaldrich.com)
  • assay has become widely employed to quantify stem-like cells in a population. (bioportfolio.com)
  • This approach removes cell aggregation and potentially shortens a 5- to 14-day assay to a 24 hours. (bioportfolio.com)