Cell survival by cell viability. Medical search. Frequent questions

This study investigated the effect of acylated synthetic ghrelin (AG) on the survival and proliferation of human chemosensitive ovarian cancer cells (A2780) and explored some mechanisms of action with a focus on the p53 apoptotic pathway and PI3K/Akt and NF- κ B survival pathways. (hindawi.com)
However, while AG treatment had no effect on control cell viability, it significantly increased cell viability and proliferation and inhibited cell death in Cis-treated cells. (hindawi.com)
Among them and over decades of research, it was shown that the PI3K/Akt/mTOR pathway is one of the most frequently hyperactive pathways in OC as well as other types of solid tumors and is associated with increased cell growth, proliferation, migration, protein synthesis, survival, and chemotherapy resistance [ 7 - 9 ]. (hindawi.com)
Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)
MTT analysis, PI/Annexin V staining, transwell and metabolic flux assays were performed to examine the effects of C1QBP on proliferation, apoptosis, migration, invasion, and oxidative phosphorylation of HCC cells. (jcancer.org)
Loss-of-function assays showed that proliferation, migration and invasion of HCC cells were mitigated while cell apoptosis was augmented upon the loos of C1QBP. (jcancer.org)
Recent studies have revealed that AXL contributes to leukaemic phenotypes through activation of oncogenic signalling pathways that lead to increased cell migration and proliferation. (cancerindex.org)
Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
Metformin has been shown to have antiproliferative properties in several cancer cell lines, possibly related to its ability to inhibit cell proliferation pathways. (endocrine-abstracts.org)
Accordingly, we aimed to evaluate the effects of metformin in the survival and proliferation of PHEO cells. (endocrine-abstracts.org)
The effects of metformin on cell proliferation markers were analyzed by western blot. (endocrine-abstracts.org)
Moreover, metformin treatment reduced the activation of proteins of the AMPK/PTEN/AKT/mTOR pathway, which suggests growth and cell proliferation impairment. (endocrine-abstracts.org)
B ) Morphology of induced pluripotent stem cells (iPSC) colonies, ( C ) proliferation rate of iPSCs, and ( D ) photoreceptor primary cilium of organoids were compared between WT and rd16 . (elifesciences.org)
We have conducted extensive in vitro studies to confirm its efficacy in supporting stem cell viability, proliferation and new tissue deposition. (ca.gov)
These scaffolds combine the injectability and cell encapsulation of conventional hydrogels with macroporosity, which facilitates nutrient transfer, cell survival, proliferation, and tissue formation. (ca.gov)
These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. (scirp.org)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)

Conclusion: For the protection of stem cells against apoptosis, different pathways, namely Phosphoinositide 3-Kinase (PI3K/AKT), Hypoxia-Inducible Factor (HIF1), Mitogen-Activated Protein Kinases (MAPK) and Hippo, are discussed. (benthamscience.com)
The most studied form of PCD is apoptosis -- a process of cellular self-destruction, distinguished by fragmentation of nuclear DNA and cell shrinkage. (sciencedaily.com)
It was named necroptosis, as it shares some characteristics of apoptosis, such as rigorous gene-level regulation, and necrosis, an inflammatory cell death in which the cell membrane swells and later breaks, leaking its contents into the intercellular space. (sciencedaily.com)
In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
Complement C11 binding protein (C1QBP, also named p32 and HABP1) is a multifunctional protein which plays a pivotal role in diverse cellular processes such autophagy and cell apoptosis [ 7 , 8 ]. (jcancer.org)
Resveratrol could play a toxic role through inducing apoptosis of the cancer cell in a time- and concentration-dependent manner. (mdpi.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combina tion or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). (scirp.org)
After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. (scirp.org)
These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. (scirp.org)
In summary, cell viability was decreased and apoptosis (initial, mid and late) was in creased after hCG and/or Ang-(1 - 7) peptides treatments. (scirp.org)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)

By coupling efficient chemical synthesis routes to multiple upfront in parallel phenotypic screens, we identify that KBU2046 inhibits cell motility and cell invasion in vitro. (nature.com)
Efficacy of KBU2046 is demonstrated across several different in vitro models and across multiple murine models of human cancer metastasis, which includes decreased metastasis, decreased bone destruction, and prolonged survival. (nature.com)
Using our recently described assay for efficiently evaluating biomass production and formulation protocols, we demonstrate the effectiveness, in vitro, of UV protectant compounds lignin (PC 1307) and Blankophor BBH in reducing OH 131.1 morbidity when cells were exposed to UV light from artificial sunlight. (usda.gov)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
These results suggest that IRE1α activity is not essential for viability in most tumor cell lines, in vitro, and that interfering with the survival functions of the UPR may not be an effective strategy to block tumorigenesis. (rcsb.org)
Radiosensitisation caused by titanium dioxide nanoparticles (TiO 2 -NPs) is investigated using phantoms (PRESAGE ® dosimeters) and in vitro using two types of cell lines, cultured human keratinocyte (HaCaT) and prostate cancer (DU145) cells. (medsci.org)
Comparable levels of radiosensitisation were observed in both phantoms and cells at kilovoltage ranges of x-ray energies (slightly higher in vitro) . (medsci.org)
In a very interesting review, P. MARRACK & J. KAPPLER focus on naïve, activated, and memory T cells survival separately, and discuss experiments done in vitro and in mice lacking or containing normal populations of lymphocytes. (scielo.br)
Here, we combine in-vitro structural measurements, which used EPR spectroscopy and UV-Vis measurements, with cell experiments to explore the role of the various methionine residues in CusB. (biorxiv.org)
Using in vitro ibrutinib-resistant models and cells from patients with CLL, we show that pirtobrutinib potently inhibits BTK-mediated functions including B-cell receptor. (lu.se)
Using in vitro ibrutinib-resistant models and cells from patients with CLL, we show that pirtobrutinib potently inhibits BTK-mediated functions including B-cell receptor (BCR) signaling, cell viability, and CCL3/CCL4 chemokine production in both BTK wild-type and C481S mutant CLL cells. (lu.se)
The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)

MTT, Transwell, scratch, western blotting and reverse transcription‑quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR‑30a‑5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. (spandidos-publications.com)
Methods: Cell viability was evaluated by MTT and TRIPAN assays using the PC12-Adh PHEO cell line treated with metformin in increasing concentrations (0 30 mM). (endocrine-abstracts.org)
Clonogenic and cell viability assays were employed to determine cells survival curves from which the dose enhancement levels "radiosensitisation" are deduced. (medsci.org)
Cell culture infectivity assays and animal models were not available to determine the infectivity or viability of the oocysts. (cdc.gov)

It has been reported that miR-30a-5p expression is decreased in primary gallbladder cancer (GBC) lesions, and inhibition of its expression in GBC cells promoted migration and invasion ( 8 ). (spandidos-publications.com)
Authors showed that a fraction from D. tertiolecta CCMP364 containing 95% of violaxanthin displayed 50% growth inhibition on the MCF7 breast cancer cell line at a concentration of 11.7 μg/mL. (frontiersin.org)
Results and Discussion: Metformin at 20 mM induced an inhibition of 60% of cell viability after 48 h treatment, as compared to untreated controls, and increased cellular lipid peroxidation, while decreased O 2 consumption in PC12-Adh PHEO cells. (endocrine-abstracts.org)
Inhibition of PFKFB3 displays a cancer-specific synergy with platinum compounds in blocking cell viability and restores sensitivity in treatment-resistant models. (nki.nl)
At time of progression, these primary CLL cells show increasing resistance to pirtobrutinib in signaling inhibition, cell viability, and cytokine production. (lu.se)
CONCLUSIONS: We concluded that AS treatment suppresses EMT/metastasis and Warburg effects through Twist and HIF-1α inhibition in Twist-overexpressing HNSCC cells. (bvsalud.org)
KSP inhibition caused tumor regression in vivo and resulted in increased survival of multiple PDX models. (lu.se)

Glioblastoma multiforme (GBM) is the most prevalent primary malignant brain tumor and, remains one of the most lethal cancers, with a median survival of only 15 months upon diagnosis 1 . (nature.com)
Clear cell renal cell carcinoma (ccRCC) is the seventh most frequently diagnosed tumor in adults in Europe and represents approximately 2.5% of cancer deaths. (cancerindex.org)
The molecular biology underlying renal cell carcinoma (RCC) development and progression has been a key milestone in the management of this type of tumor. (cancerindex.org)
Calebin-A induced death of malignant peripheral nerve sheath tumor cells by activation of histone acetyltransferase. (cancerindex.org)
Screening of native tumor cell lines (>300) against selective IRE1α inhibitors failed to demonstrate any effect on cellular viability. (rcsb.org)
Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
11 Chemoresistance, either acquired or intrinsic, is associated with a complicated and multifactorial process, including decreases of intracellular drugs, changes of drug targets, aberrant regulation of cell survival or death, and crosstalks between tumor microenvironment and cancer cells. (dovepress.com)
For this work, I have used patient-derived xenograft mouse models and neuroblastoma cells grown as 3D tumor organoids. (lu.se)
In vivo, combination treatment resulted in slower tumor growth and increased survival of neuroblastoma PDX models. (lu.se)
In vivo, rigosertib treatment resulted in decreased tumor growth and increased survival of PDX mice. (lu.se)

a potent inhibitor of cell viability in breast carcinoma cells expressing PAR1. (tufts.edu)

Neurotrophins also induce differentiation of progenitor cells, to form neurons. (wikipedia.org)
In the peripheral and central neurons, neurotrophins are important regulators for survival, differentiation, and maintenance of nerve cells. (wikipedia.org)
it helps to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses through axonal and dendritic sprouting. (wikipedia.org)
A ) Morphology of Nrl -GFP wild-type (WT) and rd16 retinal organoids differentiated from mouse-induced pluripotent stem cells (iPSC) at various differentiation time points. (elifesciences.org)

While many pathways have been shown to regulate cell motility, they constitute pathways whose regulatory effects are pleiotropic 5 . (nature.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
Copper is required for many biological pathways, but sometimes it can harm the cell. (biorxiv.org)

Aifantis, I. Regulation of stem cell function by protein ubiquitylation. (benthamscience.com)
Dr Suzuki proposes that one of the possible mechanisms triggering necroptosis is the suppression of degradation of messenger RNA (mRNA), which transports the encoded protein sequence (the message) from the cell nucleus to the ribosomes in the cytoplasm, where the proteins are actually synthesised. (sciencedaily.com)
Analysis of other components of the CCR4-NOT complex in CNOT3-deficient cells suggests that this protein plays a vital role in maintaining the structural and functional integrity of the entire complex and that it is crucial for cell viability. (sciencedaily.com)
moreover, only normal, intact CNOT3 protein can rescue dying cells' viability and growth. (sciencedaily.com)
A mutant form of the protein, missing its C-terminal amino acids -- a docking station for other proteins of the CCR4-NOT complex -- was unable to aid cell survival. (sciencedaily.com)
Nerve growth factor (NGF), the prototypical growth factor, is a protein secreted by a neuron's target cell. (wikipedia.org)
mRNA of ghrelin receptors(GHS-R1a and GHS-R1b), as well as, protein levels of GHS-R1a, were expressed abundantly in A2780 cells. (hindawi.com)
AG treatment did not affect the mRNA and protein levels of GHS-R1a and GHS-R1b in both control and Cis-treated cells. (hindawi.com)
In both control and Cis-treated cells, AG treatment significantly increased PI3K/Akt/mTOR signaling and enhanced the nuclear accumulation of NF- κ B. Concomitantly, in both control and Cis-treated cells, AG significantly lowered the protein levels of p53, p-p53 (Ser16), PUMA, cytochrome C, and cleaved caspase-3. (hindawi.com)
Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Hou G, Lu Z, Wang Z, Yang X. The Mitochondrial Protein C1QBP Promotes Hepatocellular Carcinoma Progression by Enhancing Cell Survival, Migration and Invasion. (jcancer.org)
"It can be expected that the protein matrix would have different cell release properties than the other ME methods (polymer or fat based)," said Champagne. (nutraingredients-usa.com)
Rosell Inc.), encapsulated in a whey protein isolate (WPI, Darisco Intl.). Beads were prepared by extruding the denatured WPI-concentrated bacteria solution, and 96 per cent of the probiotic cells were in the whey protein particles. (nutraingredients-usa.com)
Angiotensin II Activates Extracellular Signal Regulated Kinases via Protein Kinase C and Epidermal Growth Factor Receptor in Breast Cancer Cells," Journal of Cellular Physiology, Vol. 196, No. 2, 2003, pp. 370-377. (scirp.org)
AS exposure inhibited Twist and hypoxia-inducible factor-1α (HIF-1α) protein and/or mRNA expression in Twist-overexpressing OECM-1 and FaDu-Twist cells. (bvsalud.org)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

The role of the mTOR pathway in estrogen response was evaluated in aromatase-expressing MCF7/Aro breast cancer cells by immunoblotting. (aacrjournals.org)
Treatment of MCF7/Aro cells with estradiol or androstenedione caused modulation of the mTOR pathway, a phenomenon reversed by letrozole or RAD001. (aacrjournals.org)

Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)

Across three different murine models of human prostate and breast cancer, KBU2046 inhibits metastasis, decreases bone destruction, and prolongs survival at nanomolar blood concentrations after oral administration. (nature.com)

Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
However, the viability of leukocytes after cryopreservation varied significantly (p (cdc.gov)
AXL significantly promotes tumour cell migration, invasion and metastasis, as well as angiogenesis. (cancerindex.org)
Advancing your pluripotent stem cell therapy research to clinical applications requires careful material selection as the quality of starting materials significantly impacts the properties of your final stem cell therapy product. (thermofisher.com)
In this study, we demonstrated that NSCLC-derived CAFs were innately resistant to cisplatin treatment and CAFs-conditioned medium significantly promoted the survival rate of NSCLC cells after cisplatin treatment. (dovepress.com)
5 To date, platinum-based chemotherapy is the most effective therapy to treat advanced NSCLC patients, 6 with a significantly higher survival and response rate. (dovepress.com)

In spite of the big improvement in the clinical field for OC therapy, an overlall low survival and high recurrence rates still exist and were attributed to the poor screening approach, inadequate therapeutic options, and development of drug resistance [ 3 , 4 ]. (hindawi.com)
Although patients with HCC have been benefiting from the development of various effective therapeutic approaches, the five-year survival rate is still rather low (around 12% as reported in China) [ 2 ]. (jcancer.org)
We identify a unique cell motility regulatory mechanism and synthesize a targeted therapeutic, providing a platform to pursue studies in humans. (nature.com)
Processes that drive the development of increased cell motility represent high-value therapeutic targets. (nature.com)
We hypothesized that we could use them to identify novel and selective sites that regulate cancer cell motility and that such sites would constitute high-value therapeutic targets. (nature.com)
Our CTS products have been used in over 200 clinical trials as well as in commercially approved cell therapies including FDA-approved CAR-T therapies and the first FDA-approved therapeutic cancer vaccine. (thermofisher.com)
Impact Providing the immediate cell therapeutic candidate for clinical trial of diabetic patients. (ca.gov)
Research Objective The expected outcome is a therapeutic candidate, allogeneic HSC-engineered HLA-I/II-negative human iNKT cells, that can potentially be used as an off-the-shelf cellular therapy for treating cancer. (ca.gov)
The long-term goal of this grant is to accelerate clinical translation of stem cell-based therapy for regenerative medicine by enhancing cell delivery, survival, and integration, thus improving therapeutic outcomes, substantially reducing the number of cells needed for transplantation, and reducing the associated time and cost to produce these cells. (ca.gov)
The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
MATERIALS AND METHODS: MTT assay, Western blot, migration/invasion assay, immunofluorescence, glucose uptake assay, lactate assay, oxygen consumption rate (OCR)/Extracellular acidification rate (ECAR) assay, Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS), and qRT-PCR experimental techniques were used to evaluate the therapeutic potential of AS treatment in HNSCC cells. (bvsalud.org)
Despite methodological differences, findings evidence that although IBC presents particular features (lower survival rate and worse prognostics than most types of breast cancer), very few studies examine its epidemiology and specific risk factors in depth and use any other therapeutic approaches than those commonly used for other breast cancer subtypes. (bvsalud.org)

The efficiency of transplanted stem cells reduces due to conditions like hypoxia, inflammation, nutrient deprivation, immunogenicity, extracellular matrix loss on delivery and mechanical stress. (benthamscience.com)
Discussion: Activation of the PI3K/AKT pathway decreases the concentration of apoptotic factors, while the HIF pathway protects stem cells against the micro-environment of tissue (hypoxia). (benthamscience.com)
In their studies on genetically modified mice, the Bad Nauheim researchers also found that in animals in which miR-1/133a was switched off, the heart muscle cells coped much better with hypoxia conditions (oxygen depletion), such as those that exist after a heart attack. (mpg.de)
In the experiment, the heart muscle cells from miR-1/133a knockout animals showed higher viability and survival in hypoxia,' Valussi said. (mpg.de)
AS decreased the ECAR, and enhanced the OCR together with basal respiration, ATP production, maximal respiration, and spare respiratory capacity under normoxia and hypoxia (CoCl2) in OECM-1 cells. (bvsalud.org)

Methods: This article focuses on recent research development that addresses the viability issues of stem cells. (benthamscience.com)
Developing methods for producing cells of biocontrol agents that are effective and can be successfully incorporated into a biological control product requires considerable research effort. (usda.gov)
A variety of methods exist for collecting buccal cells from the oral cavity, including rinsing with saline, mouthwash, or scraping the oral cavity. (cdc.gov)
Attempts were made to assess the viability of the inoculum by observing sporozoite motility after excystation of the oocysts by different methods. (cdc.gov)
However, these methods did not yield a sufficient number of motile sporozoites to measure viability. (cdc.gov)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)

We found that miR-1/133a suppresses two receptors on heart muscle cells. (mpg.de)

Human livers declined for transplantation were perfused using a red blood cell-based perfusate under normothermic conditions (36 °C) and then split and simultaneously perfused on separate machines. (nih.gov)
Research Objective Determine optimal islet transplant conditions and systemic treatments that promote graft survival upon transplantation into immune-competent diabetic subjects. (ca.gov)
Impact Our proposal will optimize the generation and viability of an unlimited, reproducible source of human engineered islets for transplantation. (ca.gov)
However, the majority of transplanted cells die shortly after transplantation, which greatly diminishes the efficacy of stem cell-based therapies. (ca.gov)

The results demonstrated that miR‑30a‑5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. (spandidos-publications.com)
Curcumol treatment was identified to increase miR‑30a‑5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. (spandidos-publications.com)
Overexpression of miR‑30a‑5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. (spandidos-publications.com)
In the present study, we found that matrix metalloprotease-1 (MMP-1) activates the PAR1-Akt survival pathway in breast carcinoma cells. (tufts.edu)

4 As the primary type of lung cancer, non-small cell lung cancer (NSCLC) exhibits multiple histological types, such as bronchoalveolar carcinoma, large cell carcinoma, squamous cell carcinoma, and adenocarcinoma. (dovepress.com)
AIM OF THE STUDY: In this study, we investigated the metabolic reprogramming and anti-metastasis/epithelial-mesenchymal transition (EMT) effects of AS exposure in Twist-overexpressing head and neck squamous cell carcinoma (HNSCC, OECM-1 and FaDu-Twist) cells. (bvsalud.org)
OBJECTIVES: The aim of this pilot study is to identify the genetic factors that contribute to the response of metronomic chemotherapy in head and neck squamous cell carcinoma (HNSCC) patients using whole-exome sequencing (WES). (bvsalud.org)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Although drugs, for example a MEK inhibitor Refametinib [ 5 ], targeting key driver genes involved in HCC progression are being utilized for the clinical intervene, chemotherapy-resistance and the following cancer recurrence or metastasis still hinder the improvement of patient survival rates. (jcancer.org)

This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. (cancerindex.org)
This effect is associated with enhanced lipid peroxidation and decreased O 2 consumption by those cells, which are both suggestive of increased cellular stress. (endocrine-abstracts.org)
provides an overview of the dynamic in vivo life of NK cells from their development in the bone marrow to their mature cellular responses in the periphery and their ultimate demise, with emphasis on mouse NK cells and viral infections. (scielo.br)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)

Ramalingam, M. Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine. (benthamscience.com)
Accordingly, in contrast to organs with a high regenerative capacity, no divisible, regenerative stem cells are found in the heart. (mpg.de)
Despite the great promise stem cells hold for regenerative medicine, the efficacy of stem cell-based therapies is greatly limited by poor cell engraftment and survival. (ca.gov)

Melton, D.A. "Stemness": Transcriptional profiling of embryonic and adult stem cells. (benthamscience.com)
In particular, the transcriptional coactivator YAP1 rescued cell viability in KRAS-dependent cells upon suppression of KRAS and was required for KRAS-induced cell transformation. (nih.gov)

C1QBP is correlated with a poor prognosis of HCC patients and promotes the survival, migration and invasion of HCC cells. (jcancer.org)
It has been reported that numerous miRNAs, including the miR-30 family, serve important roles in the regulation of cancer cell migration and invasion ( 5 - 7 ). (spandidos-publications.com)
We have observed that ectopic expression of PAR1 in MCF-7 breast carcinoma cells induces a fibroblast-like morphological change and a corresponding enhancement of migration and invasion. (tufts.edu)

Efficient recognition of foreign pathogens by T cells requires adhesive interactions between T cells and other cell types, such as endothelial cells and antigen-presenting cells (APCs), and with components of the extracellular matrix. (scielo.br)

Neurotrophins are a family of proteins that induce the survival, development, and function of neurons. (wikipedia.org)
It can be used alone to induce regeneration by stem cells from the body, or in combination with transplanted stem cells. (ca.gov)
Together, these observations suggest that PAR1 and EGFR cooperatively induce Epithelial-to-Mesenchymal Transition (EMT) in breast carcinoma cells. (tufts.edu)

Similar survival rates were found for leukocytes (88%) and epithelial cells (10%) after rinsing with Listerine mouthwash. (cdc.gov)

A recent paper published in Scientific Reports by researchers from Okinawa Institute of Science and Technology graduate University (OIST), establishes that in mouse cells, proteins comprising the CCR4-NOT complex are actively involved in regulation of a form of PCD called necroptosis. (sciencedaily.com)
Increased cancer cell motility constitutes a root cause of end organ destruction and mortality, but its complex regulation represents a barrier to precision targeting. (nature.com)
Here, we identify novel regulation of FA repair by the cancer-associated glycolytic enzyme PFKFB3 that has functional consequences for replication-associated ICL repair and cancer cell survival. (nki.nl)

Stem cells differentiate into different types of cells, such as neural, hematopoietic, adipose, etc. and are used for the treatment of various conditions like myocardial infarction, spinal cord injury, Parkinson's disease and diabetes. (benthamscience.com)
They belong to a class of growth factors, secreted proteins that can signal particular cells to survive, differentiate, or grow. (wikipedia.org)
In the B cell system protective memory is mediated by plasma cells that secrete antibodies, whereas reactive memory is mediated by memory B cells that proliferate and differentiate to plasma cells in response to secondary antigenic stimulation. (scielo.br)
MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)

Another well-known cell suicidal programme is autophagy, in which a cell cannibalises itself by degrading its proteins into amino acids. (sciencedaily.com)
In the CNOT3-depleted cells the stability and expression of mRNAs encoding RIPK1 and RIPK3 proteins, which are directly associated with necroptosis, are increased. (sciencedaily.com)
This leads to build-up of death-triggering proteins and cell demise. (sciencedaily.com)
They are small proteins that secrete into the nervous system to help keep nerve cells alive. (wikipedia.org)

Bach, E.A. JAK/STAT signaling in stem cells and regeneration: From drosophila to vertebrates. (benthamscience.com)
The research in this program includes developmental biology studies on tissue formation, particularly the development and regeneration of pancreatic islets, and basic biology studies on hormone synthesis and secretion from pancreatic endocrine cells (alpha, beta, delta, etc.) within the islet. (nih.gov)
In the future, miR-1 and miR-133 could be used to specifically switch on cell division in the heart, for example to stimulate heart muscle regeneration after a heart attack. (mpg.de)
Clearly, the absence of miR-1/133a had a positive effect on myocardial cell survival and regeneration. (mpg.de)
Meanwhile, we will also start assessing the efficacy of µRB-based scaffolds for supporting stem cell engraftment and bone regeneration in a murine cranial defect model. (ca.gov)
Stem cell-based therapies hold great promise for treating tissue loss and enhancing tissue regeneration, often via direct injection of cells at the target site. (ca.gov)

Research Objective We propose to generate NK cells with enhanced immunity from gene-edited human PSCs and use the resultant NK cells to kill SARS-CoV-2-infected cells to combat against COVID-19. (ca.gov)
Research Objective Universal Pluripotent Liver Failure Therapy (UPLiFT) is composed of 2 lines- UPLiFT0 ( from LiPSC-GR1.1) and UPLiFT1 which will be derived from gene edited universal human pluripotent stem cells. (ca.gov)
Research Objective We will produce a universal donor cell (UDC) line by gene editing an embryonic stem cell line. (ca.gov)

Despite the fact that most follicular lymphomas are advanced at the time of diagnosis, many patients may not require treatment for prolonged periods of time, and the median survival of patients with follicular lymphomas has historically been approximately 8-10 years. (medscape.com)

This proposed mechanism is supported by empirical evidence from the NCI-60 panel of breast carcinoma cells, which demonstrate 100% correlation of PAR1 expression with EGFR expression and EMT status. (tufts.edu)
We demonstrate that primary CLL cells from responding patients on the pirtobrutinib trial show reduced BCR signaling, cell survival, and CCL3/CCL4 chemokine secretion. (lu.se)

Zhang, Y. Integrin β1 increases stem cell survival and cardiac function after myocardial infarction. (benthamscience.com)

Prognostic value of EVDR of ascites samples in PFS (Progression-free survival) prediction [Time Frame: From diagnosis until month 6 (12) or relapse/ EoS, whatever comes first. (who.int)

They possess intricate molecular mechanisms that promote either suicide or survival. (sciencedaily.com)
It is one step toward deciphering the mechanisms of cell death," says Dr Toru Suzuki, group leader at the Cell Signal Unit, led by Prof. Tadashi Yamamoto. (sciencedaily.com)

In conclusion, the findings of this study show that AG promotes cell survival of the OC cells and renders them resistat to Cis therapy, an effect that is mediated by the activation of PI3K/Akt/mTOR and activation of NF- κ B, and requires GHS-R1a. (hindawi.com)

Current machine perfusion technology permits livers to be preserved ex situ for short periods to assess viability prior to transplant. (nih.gov)

Results: To increase the viability of stem cells, techniques like scaffolds of stem cells with hydrogel or alginate, pre-conditioning, different routes of administration and encapsulation, are implemented. (benthamscience.com)
To overcome this major bottleneck, the goal of this proposal is to validate the efficacy of novel microribbon (µRB)-based scaffolds for cell delivery. (ca.gov)
In preliminary studies, our µRB-based scaffolds markedly enhanced the survival of human stem cells and accelerated bone repair in vivo. (ca.gov)

NGF is released from the target cells, binds to and activates its high affinity receptor TrkA on the neuron, and is internalized into the responsive neuron. (wikipedia.org)
In some cases, cisplatin+AG-treated cells were pre-incubated either with [D-Lys3]-GHRP-6, a ghrelin receptor antagonist, or with LY294002, a PI3K inhibitor. (hindawi.com)
The two molecules, oncostatin M receptor and FGF receptor 1, control the division activity of heart muscle cells in the adult heart,' says Thomas Böttger, one of the lead authors of the study. (mpg.de)
Indeed, the expression of the genes for the oncostatin M receptor and the FGF receptor 1 increased in these cells, and with it the number of receptor molecules. (mpg.de)

Bioinformatic approaches were employed for checking the expression of C1QBP in HCC patient samples and the association between C1QBP mRNA expression and survival rates of patients with HCC or the promoter methylation of C1QBP . (jcancer.org)

In the current study, the antiproliferative activity of raw extracts and fractions of D. tertiolecta (clone CCMP 1320) was evaluated against four different human cancer cell lines: melanoma, hepatocellular liver carcinoma, and two lung adenocarcinoma cell lines. (frontiersin.org)

Extensive research has shown that OC cells undergo significant metabolic alterations during tumorigenesis. (stanford.edu)
This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
RESULTS: This study showed that AS exhibits anti-EMT and anti-metastatic effects as well as metabolic reprogramming in Twist-overexpressing HNSCC cells. (bvsalud.org)

Background: Stem cells are of two types: embryonic and adult stem cells and they act as a repair system by replenishing body tissue. (benthamscience.com)

Such cells control their degree of innervation (the number of axon connections) by the secretion of various specific neurotrophic factors that are essential for neuron survival. (wikipedia.org)

Human A2780 ovarian cancer cells were cultured with or without AG treatment in the presence or absence of cisplatin. (hindawi.com)
Cancer cells that express oncogenic alleles of RAS typically require sustained expression of the mutant allele for survival, but the molecular basis of this oncogene dependency remains incompletely understood. (nih.gov)
Tumour radiosensitivity can be modified by agents, which enhance the radiation effects in the tumour, with the objective of delivering efficient radiation doses that can eradicate cancer cells without exceeding normal tissue tolerances [ 5 ]. (medsci.org)
Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)

however, processes aiding cell survival have not been well understood. (sciencedaily.com)
"The WPI gel has the potential to increase the survival rate of the microorganism to food processes and during storage and could be used to add probiotic cultures in a wide range of functional food applications," wrote lead author A. Ainsley Reid. (nutraingredients-usa.com)
This triggered intracellular processes, at the end of which cell division was initiated in the cardiac muscle cells. (mpg.de)

The authors also argue that memory B and T cell responses are long-lived in the absence of antigen, but they are modulated and influenced by other infections and that memory B and T cells can either provide protective immunity or contribute to immunopathology on infection with homologous or heterologous viruses. (scielo.br)
discuss the role of integrins in T cell- mediated immunity, with a focus on how these receptors participate in lymphocyte recirculation and T cell activation, how antigen stimulation regulates integrin activity, and how integrins define functionally unique subsets of T cells and APCs. (scielo.br)

The knockdown of ATG9B expression in GBM cells suppressed TMZ-induced autophagy as well as TMZ-resistance. (nature.com)

This work will provide researchers in the field of formulating microbial cells with new ideas for conducting such work. (usda.gov)
In order to obtain a bacteria population of 10 million cells per gram, the researchers added 1.1 per cent weight for weight to the foods. (nutraingredients-usa.com)
The researchers prepared semisweet biscuits using the microencapsulated probiotic, and also compared the viability of the bacteria in refrigerated vegetable juice and a frozen cranberry juice. (nutraingredients-usa.com)
However, the researchers report that only four per cent of the ME cells were viable in the biscuits after 24 hours storage at 23 degrees. (nutraingredients-usa.com)
The researchers also state that theirs is the first report of viability levels in frozen cranberry juice. (nutraingredients-usa.com)
More encouraging results were obtained when the entrapped probiotics were added to vegetable juice, said the researchers, with 33.4 per cent of the cells still viable after a two-week storage period, compared to only 6.6 per cent of non-encapsulated cells. (nutraingredients-usa.com)
To investigate the background in more detail, the Max Planck researchers used heart muscle cells in which miR-1/133a could be temporarily inactivated by genetic engineering. (mpg.de)

Growth factors such as neurotrophins that promote the survival of neurons are known as neurotrophic factors. (wikipedia.org)
The aim of this work was to study the growth and survival of ATR L. monocytogenes strain Scott A in media acidified with malic acid, lactic acid, or blueberry extract. (iospress.com)

We use the unique characteristics of small molecules to probe and selectively modulate cell motility. (nature.com)
It has therefore not been possible to identify regulators of cell motility possessing the selective capacity to support targeted manipulation. (nature.com)

For example, neurodegeneration results from too many cells being culled by PCD and leads to incurable diseases such as Huntington's, Alzheimer's, and Parkinson's. (sciencedaily.com)
These results suggest that some human inflammatory diseases associated with necrotic cell death, such as rheumatoid arthritis and psoriasis, may be caused by deregulation of the CCR4-NOT complex. (sciencedaily.com)
Our results suggest that metformin has a moderate inhibitory effect on the viability of PC12-Adh PHEO cells. (endocrine-abstracts.org)
This results in an incapacity to replicate cells to progress through S-phase, an accumulation of DNA damage in replicating cells, and fork collapse. (nki.nl)
Taken together, these results suggest that CAFs-derived exosomes confer cisplatin resistance of NSCLC cells through transferring miRNA-130a and that PUM2 is a critical factor for packaging miRNA-130a into exosomes. (dovepress.com)

Conversely, overexpression of miR-1/133a caused the cells to terminate any cell division activity if we had previously induced it by administering the binding molecules of the two receptors, i.e. oncostatin and FGF,' explains Melissa Valussi, first author of the study. (mpg.de)
In this case, the heart muscle cells lost the ability to contract due to the unchecked activity of the two receptors and the animals suffered heart failure. (mpg.de)

Scientists from the Max Planck Institute for Heart and Lung Research in Bad Nauheim have now discovered that the two micro RNA molecules miR-1 and miR-133 in the adult heart inactivate two genes that normally force cell division. (mpg.de)
To identify genes that can functionally substitute for oncogenic RAS, we systematically expressed 15,294 open reading frames in a human KRAS-dependent colon cancer cell line engineered to express an inducible KRAS-specific shRNA. (nih.gov)
We found 147 genes that promoted survival upon KRAS suppression. (nih.gov)

Similar drops in viability of the cells was observed, as with the biscuits, despite the encapsulated cells having higher survival than free cells. (nutraingredients-usa.com)

Although NK cells play important roles in host defense against various infectious agents, they are critical in host defense against viral infections. (scielo.br)

Recent basic and clinical research has revealed that hydrogen is an important physiological regulatory factor with antioxidant, anti-inflammatory and anti-apoptotic protective effects on cells and organs. (researchgate.net)

Research Objective These studies will produce a new CAR-targeted iPSC-derived macrophage-based cell therapy product for treatment of refractory malignancies such as ovarian cancer. (ca.gov)

Despite the apparent survival advantage conferred by PSA screening, the US Preventive Services Task Force (USPSTF) recommends against screening for prostate cancer in men aged 75 years or older. (medscape.com)

Stem Cell Rev. Rep. , 2019 , 16 (1), 103-117. (benthamscience.com)
At Thermo Fisher Scientific, we have developed Gibco Cell Therapy Systems (CTS) products across the pluripotent stem cell therapy workflow to ease the transition from discovery to commercial manufacturing. (thermofisher.com)
Based on the widely cited Essential 8 Medium, Gibco CTS Essential 8 Medium is the first globally available human pluripotent stem cell (hPSC) culture medium with components not directly derived from animals. (thermofisher.com)
Karyotypes of induced pluripotent stem cell lines. (elifesciences.org)
In the next report period, we will focus on measuring the functional improvement by monitoring stem cell survival using BLI and fat volume retention using micro-CT imaging over time. (ca.gov)

The present study revealed that miR‑30a‑5p expression was downregulated in human CRC tissues and cells. (spandidos-publications.com)
Buccal cells are becoming a widely used tissue source for monitoring human exposure to occupational and environmental genotoxicants. (cdc.gov)
A novel human anti-AXL monoclonal antibody attenuates tumour cell migration. (cancerindex.org)
Research Objective Functional human islet like organoids differentiated from human pluripotent stem cells. (ca.gov)
Although homologs of CopA and CueO also exist in the human cells ATP7b and SOD, respectively, the CusCFBA and CueR systems are unique to prokaryotic systems. (biorxiv.org)

Anatomy20

Organisms11

Diseases25

Chemicals and Drugs93

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes63

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science4

Health Care16

Proliferation18

Apoptosis14

Vitro12

Assays4

Inhibition7

Tumor10

Inhibitor of cell1

Differentiation4

Pathways3

Protein17

MCF72

Cycle arrest2

Inhibits1

Significantly6

Therapeutic13

Hypoxia5

Methods5

Adhesion1

Suppresses1

Transplantation4

Pathway4

Squamous cell carc3

Metastasis2

Cellular4

Regenerative3

Transcriptional2

Invasion3

Endothelial cells1

Induce3

Epithelial cells1

Regulation3

Differentiate4

Proteins4

Regeneration6

Gene3

Median survival1

Demonstrate2

Myocardial1

Progression1

Mechanisms2

MTOR1

Assess1

Scaffolds3

Receptor4

Approaches1

Hepatocellular1

Metabolic4

Embryonic1

Secretion1

Cancer cells6

Processes3

Antigen2

Autophagy1

Researchers7

Growth2

Motility2

Results5

Receptors2

Genes3

Higher survival1

Play important roles in host1

Apoptotic1

Ovarian cancer1

Prostate1

Stem cell5

Human5