Cell subcellular fractions. Medical search. Frequent questions

Using the endothelin-1 (ET-1)-stimulated elevation in cGMP in LLC-PK1 cells as a biological detector system for the conversion of big ET-1 (bET-1) to ET-1, we detected bET-1-converting activities in subcellular fractions from bovine aortic cultured endothelial cells (BAE) and rat brain. (aspetjournals.org)

Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
Loading controls can be general whole cell proteins or specific markers for subcellular fractions (e.g. nucleus, mitochondria, membrane). (novusbio.com)
These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
Gimap proteins may therefore have similar function, but different subcellular locations. (hindawi.com)
Proteins that are found exclusively or predominantly in a sub-cellular location will be more enriched in a lysate of the sub-cellular fraction compared with whole cell or tissue lysates. (abcam.com)
This is a popular buffer for studying proteins that are cytoplasmic or membrane-bound, or for whole cell extracts. (abcam.com)
RIPA buffer is useful for whole cell extracts and membrane-bound proteins, and may be preferable to NP-40 or Triton X-100-only buffers for extracting nuclear proteins. (abcam.com)
However, the majority of studies that report absolute abundances of enzymes and transporter proteins do so in enriched membrane fractions, to increase the abundance per sample and thus the assay's sensitivity, rather than measuring the expected lower abundance in the more biologically meaningful whole cells or tissues. (aspetjournals.org)
We initially used maps statically to generate a database with localization and absolute copy number information for over 8700 proteins from HeLa cells, approaching comprehensive coverage. (elifesciences.org)
Proteins are the tools and machines of the cell, and each organelle has its own set of proteins that it requires to work correctly. (elifesciences.org)
Each cell contains ten or more organelles, and several thousand different types of proteins. (elifesciences.org)
The location of many proteins in a cell is unclear or simply not known. (elifesciences.org)
set out to develop a method that reveals the locations of all the proteins in a cell at any given time. (elifesciences.org)
The resulting technique maps the location of most of the proteins in a human cancer cell line and, in addition, determines how many copies of each protein there are. (elifesciences.org)
Moreover, although the abundance of most protein groups reflected that of related bacterial populations, we found a specific independent regulation of bacteria-derived cell envelope proteins. (bmj.com)
10] This is the most toxic component due to high reduction-oxidation (redox) potential that generates oxygen-free radicals such as superoxide anion in the cells, which damages DNA, proteins, and membrane lipids in the cell. (medscape.com)

The objective of this study was to identify the PDE10A isoforms expressed in the testis and germ cells, and to determine the presence and localization of PDE10A in mature spermatozoa. (plos.org)
The investigation of the subcellular localization of apyrases showed that some fraction of apyrase was localized on cell periphery. (utexas.edu)
Subcellular localization critically influences protein function, and cells control protein localization to regulate biological processes. (elifesciences.org)
Protein function is critically determined by subcellular localization, as organelles offer different chemical environments and interaction partners. (elifesciences.org)
In order to regulate protein activity, many biological processes involve changes in protein subcellular localization. (elifesciences.org)

Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
We specialize in control and disease-state biospecimens including human and animal tissues, cell products, blood and other biofluids. (massbio.org)
Western blot sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration. (abcam.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
This communication discusses the balance between protein enrichment and potential loss during the preparation of membrane fractions from whole cells or tissues. (aspetjournals.org)
Used for the discharge across the cell membrane, into the extracellular space or ducts, of endogenous substances resulting from the activity of intact cells of glands, tissues, or organs. (bvsalud.org)
Animal, plant, microbial and human tissues are extracted using appropriate protocols, e.g. for adherent cells with our rapid solvent quenching, followed by solvent partitioning methods to simultaneously obtain polar, non-polar, and protein fractions .This method is optimized for metabolite coverage (including relatively labile components), reproducible recovery, a wide range of sample sizes (µg to g weight), and compatibility with both NMR and MS. (uky.edu)
As such they are principal sinks and sources of carbon, nitrogen (and phosphorus) in cells and tissues. (uky.edu)
COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)
In some patients, noticeably those with thalassemia major, sickle cell disease, myelodysplastic syndrome, aplastic anemia, hemolytic anemia, and refractory sideroblastic anemias, who may become transfusion-dependent and receive excess iron with each transfusion (that the body has no means to excrete), iron gradually accumulates in various tissues, causing morbidity and mortality. (medscape.com)
12] Unlike ferritin, it does not circulate in blood but is deposited in tissues and is unavailable when cells need iron. (medscape.com)

In bax − / − bak − / − cells, introduction of Bak mutants selectively targeted to either mitochondria or the ER can induce apoptosis. (rupress.org)
The present invention provides stem cells enriched with healthy functional mitochondria, and therapeutic methods utilizing such cells for the alleviation of debilitating conditions, including aging, and age-related diseases as well as the debilitating effects of anti-cancer therapies in subjects in need thereof. (justia.com)
The present invention relates to stem cells enriched with functional mitochondria, and therapeutic methods utilizing such cells to diminish the debilitating effects of various conditions, including aging and age-related diseases as well as the debilitating effects of anti-cancer therapy treatments. (justia.com)
Mitochondria are found in nearly all eukaryotic cells and vary in number and location depending on the cell type. (justia.com)
Mitochondria perform numerous essential tasks in the eukaryotic cell such as pyruvate oxidation, the Krebs cycle and metabolism of amino acids, fatty acids and steroids. (justia.com)
Additional processes in which mitochondria are involved include heat production, storage of calcium ions, calcium signaling, programmed cell death (apoptosis) and cellular proliferation. (justia.com)
The majority of this ATP production by non-photosynthetic eukaryotes takes place in the mitochondria, which can make up nearly 25% of the total volume of a typical cell. (justia.com)
WO 2016/135723 to the present inventors discloses mammalian bone marrow cells enriched with mitochondria for treatment of mitochondrial diseases. (justia.com)
The method involves microinjecting heterologous mitochondria into an oocyte or embryonic cell wherein the heterologous mitochondria are capable of achieving at least normal levels of mitochondrial membrane potential in the oocyte or embryonic cell. (justia.com)
WO 2013/002880 describes compositions and methods comprising bio-energetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures. (justia.com)
US 20130022666 provides compositions comprising a lipid carrier and mitochondria as well as methods of delivering exogenous mitochondria to a cell and methods of treating or reversing progression of a disorder associated with mitochondrial dysfunction in a mammalian subject in need thereof. (justia.com)

In: 'Control of Proliferation in Animal Cells', Cold Spring Harbor Laboratory, pp. 393-410, 1974. (uni-mainz.de)
NQ interacted with HepG2 cell DNA and reduced histone deacetylases to control cell proliferation and arrest the cell cycle at the sub-G stage. (molcells.org)
NQ induced apoptosis in HepG2 cells by activating p53-ROS crosstalk and induces epigenetic modifications leading to inhibited proliferation and cell cycle arrest. (molcells.org)
Suppressing cell cycle and proliferation rates depends on different parameters, such as DNA structural changes and inhibiting the activities of histone deacetylases (HDACs). (molcells.org)
5-ethynyl-2'-deoxyuridine (EdU) assay and colony formation assay were conducted for cell proliferation ability. (biomedcentral.com)
Circ_0084615 knockdown inhibited CRC cell proliferation, migration, invasion and angiogenesis in vitro and hampered tumorigenesis in vivo . (biomedcentral.com)
One cut homeobox 2 (ONECUT2, also named OC2) is related to the proliferation, motility and differentiation of tumor cells [ 17 ]. (biomedcentral.com)

We explored whether LPL affects (a) lipoprotein transport across bovine aortic endothelial cells or (b) lipoprotein binding to subendothelial cell matrix (retention). (jci.org)

Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al. (chunyangtech.com)
First, we identified that the addition of exogenous recombinant MTase (methylation accel), a natural catalyst, enhances the generation of the corresponding propargylated product even in the cell lysate. (bvsalud.org)
By adding ProSeAM and a recombinant MTase to the lysates derived from KO cells, we successfully characterized unprecedented nonhistone substrates of several MTases. (bvsalud.org)

The binding of αSyn to ClpP further promotes a distribution of ClpP from soluble to insoluble cellular fraction in vitro and in vivo, leading to reduced solubility of ClpP. (springer.com)
Transforming growth factor-alpha (TGF-alpha) is synthesized and transported to the cell surface as a membrane-anchored precursor (proTGF-alpha) that is converted to the soluble form by proteolytic cleavage. (nih.gov)
In the cell-free reconstitution system, the membrane-bound Suc-Ala-Ala-Ala-MCA-hydrolyzing enzyme from CHO cells was also activated by phorbol ester in the presence of the soluble fraction, ATP, and vanadate. (nih.gov)
He localized the biologic activity to a fraction of lipid soluble acids that he termed "prostaglandin," hypothesizing that these substances originate in the prostate gland. (medscape.com)
In contrast to soluble chemicals, cadmium oxide particles on a cm2/ml media basis, but are only ~50 particles can settle, diffuse, and aggregate differentially accord- times more potent when differences in delivery to adherent cells are ing to their size, density, and surface physicochemistry. (cdc.gov)

Following the OECD guideline 319B PRIMACYT now offers cryopreserved pools of liver subcellular S9 fractions from Common carp kept under controlled conditions for at least two weeks in continuous flow water. (primacyt.com)
Hepatocytes are the most prominent cells within the liver. (primacyt.com)
Subcellular liver fractions are a simple but easy to use model system to study Phase I and Phase II reactions, perfectly suited for in vitro metabolism and toxicity / detoxification studies prior to preclinical or clinical tests. (primacyt.com)
Subcellular liver fractions are a simple model system for the analysis of drug biotransformation reactions. (primacyt.com)
Sprague Dawley rat liver subcellular fractions are isolated from livers obtained from male or female SD rats. (primacyt.com)
PRIMACYT offers certified cryopreserved Sprague Dawley liver subcellular fractions (S9 and microsomes) from single and pooled donors. (primacyt.com)
In humans, malaria parasites grow and multiply first in the liver cells and later in the red blood cells. (massbio.org)

WB: HeLa cell lysate. (abcam.com)

NPEPPS has been proposed to function in a variety of processes, including metabolism of neuropeptidase, regulation of the cell cycle, and hydrolysis of proteasomal products to amino acids. (wikipedia.org)
And as the premier supplier of hepatic products, including hepatocytes and subcellular fractions, BioIVT enables scientists to better understand the pharmacokinetics and drug metabolism of newly discovered compounds and their effects on disease processes. (massbio.org)

In mammalian cells, the level of extracellular ATP (eATP) is regulated by ectoapyrases, which can hydrolyze extracellular ATP to ADP and ADP to AMP. (utexas.edu)
Scholars@Duke publication: Hierarchical regulation of mRNA partitioning between the cytoplasm and the endoplasmic reticulum of mammalian cells. (duke.edu)
The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. (chunyangtech.com)

Both vanadium solutions were primarily accumulated in the mitochondrial fraction (138 +/- 0 and 195 +/- 34 ng V/g dry tissue for metavanadate and decavanadate solutions, respectively, after 12 h exposure), rather than in cytosol. (ualg.pt)
It is concluded that, in fish cardiac muscle, the vanadium distribution is dependent on the administration of decameric vanadate, with vanadium being mainly distributed in plasma, before being accumulated into the mitochondrial fraction. (ualg.pt)

Crude extracts of biospecimens can be treated with pairs of non-encoded and encoded CS probes, each pair having a CS tag targeting different functional groups, expanding on a strategy we have shown for tagging C=O groups in A549 cell extracts using QDA and 13 CD 3 -QDA. (uky.edu)

An acute exposure of two vanadate solutions-metavanadate and decavanadate-containing different vanadate oligomers, induces different patterns of subcellular vanadium distribution in blood plasma, red blood cells (RBC), and cardiac muscle subcellular fractions of the fish Sparus aurata (gilthead seabream). (ualg.pt)

Subcellular Fractions" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (childrensmercy.org)

Proximal tubule cells exposed to PMA for 2 h demonstrated a two- to threefold increase in Na/H antiporter mRNA (mRNANa/H) abundance 4 h later. (jci.org)
Accounting for losses, with recovery factors, throughout the fractionation procedure, provides a means to correct for procedural losses enabling the scaling of protein abundance from subcellular fractions to whole cell or organ abundances. (aspetjournals.org)
Combining spatial and abundance information yielded an unprecedented quantitative view of HeLa cell anatomy and organellar composition, at the protein level. (elifesciences.org)
A central ed at higher or lower density and higher or lower sedimen- objective is to continually repeat this monitoring process to tation coefficients by various subcellular organelles and determine which agents change in abundance over time, macromolecules. (cdc.gov)

Phosphoramidon-inhibitable converting activity was also detected in the cytosolic fraction of BAE. (aspetjournals.org)
In subgenomic and genomic analyses of subcellular mRNA partitioning, we report an overlapping subcellular distribution of cytosolic/nucleoplasmic and topogenic signal-encoding mRNAs, with mRNAs of both cohorts displaying noncanonical subcellular partitioning patterns. (duke.edu)

Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)
Mol Cell Biol. (childrensmercy.org)
J Cell Biol (2003) 162 (1): 59-69. (rupress.org)
J Cell Biol (2004) 164 (7): 1021-1032. (rupress.org)
Mol Biol Cell , 22 (14), 2646-2658. (duke.edu)
Mol Biol Cell 22, no. 14 (July 15, 2011): 2646-58. (duke.edu)

Chemotaxis , a kind of taxis , is the phenomenon in which bodily cells, bacteria , and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. (wikidoc.org)
Used for the anabolic formation of chemical substances in organisms, in living cells, or by subcellular fractions. (bvsalud.org)

Nuclear transfer efficiency is enhanced by introduction of compatible cytoplasm or mitochondrial DNA (same species or similar to donor cell or nucleus). (justia.com)

The carbohydrate composition of a myeloma protein from different subcellular fractions of plasma cells. (uni-mainz.de)

The interior of every cell is highly organised, and contains many compartments, called organelles, that are dedicated to specific roles. (elifesciences.org)
The hallmark of eukaryotic cells is their compartmentalization into distinct membrane-bound organelles. (elifesciences.org)
Most sub parts and organelles of a eukaryotic cell are per definition nanostructures, and it is presumed that nano-structures, both natural and artificial should have large impact on various cell functions. (lu.se)

Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (childrensmercy.org)
The new mapping method could be used in the future to analyse the anatomy of different cell types, such as nerve cells and cells of the immune system. (elifesciences.org)

Ectopic Mcl-1 expression attenuates Bak activation and apoptosis by ABT-737 + roscovitine, whereas cells overexpressing Bcl-2 or Bcl-xL remain fully sensitive. (aacrjournals.org)
HA14-1) that disable Bcl-2, resulting in induction of apoptosis in leukemia cell lines ( 8 ). (aacrjournals.org)
The mitochondrial membrane potential (MMP), reactive oxygen species (ROS), cell cycle, apoptosis, DNA damage, and caspase 3 activity were analyzed by flow cytometry, and the expression profiles of different anti- and pro-apoptotic as well as epigenetic signals were studied by immunoblotting. (molcells.org)

Address correspondence to C. Borner, Institute of Molecular Medicine and Cell Research, Center for Biochemistry and Molecular Cell Research, Albert-Ludwigs-University Freiburg, Stefan-Meier Strasse 17, D-79104 Freiburg, Germany. (rupress.org)
In the 1960s and 1970s, the revolution of modern cell biology and biochemistry provided a series of novel techniques which became available to investigate the migratory responder cells and subcellular fractions responsible for chemotactic activity. (wikidoc.org)

The mitochondrion is a membrane bound organelle found in most eukaryotic cells, ranging from 0.5 to 1.0 μm in diameter. (justia.com)

Knockout Validated Western Blot showing lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine. (novusbio.com)
Then, we applied the principle to isotope label-free quantification with HEK293T cell lysates. (bvsalud.org)
In particular, we would like to emphasize that the use of cell lysates derived from disease-associated MTase knockouts (KOs) holds vast potential to discover functionally unknown but biologically important methylation events. (bvsalud.org)

This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

Hepatic subcellular fractions were prepared, and the protein concentration of each fraction was determined. (cdc.gov)

During this time, thanks to an interdicipline project in axonal guidance on nanoimprinted patterns, I came in contact with professor Martin Kanje at the Department of Cell and Organism Biology. (lu.se)

However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)

Pulsed electromagnetic fields enhance the induction of cytokines by peripheral blood mononuclear cells challenged with phytohemagglutinin [med. (emf-portal.org)

ICC/IF: HeLa cells. (abcam.com)
A specific band was detected for LC3B at a molecular weight of approximately 15 kDa in the parental HeLa cell line, but is not detectable in the knockout cell line. (novusbio.com)

Pooled Fish S9 fractions - a new in-vitro model metabolic System. (primacyt.com)
Static magnetic fields generated by a 0.5 T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC) [med. (emf-portal.org)
Here we develop and apply the principles of their evolution from systems principally for evaluating mechanism dosimetry in vitro and outline an approach for simulation of of toxicity to high-throughput systems for rapid and cost-effective nanoparticle particokinetics in cell culture systems. (cdc.gov)

Dynamic Organellar Maps enable the proteome-wide analysis of physiological protein movements, without requiring any reagents specific to the investigated process, and will thus be widely applicable in cell biology. (elifesciences.org)
After one year as research engineer at the department of Cell and Organism Biology I started my PhD with professor Kanje as supervisor. (lu.se)

As in somatic cells, sperm intracellular cAMP concentration is regulated by the opposite action of two enzymes: the adenylyl cyclases (AC), which synthesize cAMP from ATP, and the phosphodiesterases (PDE), which break the phosphodiester bond of cAMP to form 5'-adenosine monophosphate (AMP). (plos.org)
The ATP concentration inside the cell is typically 1-10 mM ATP can be produced by redox reactions using simple and complex sugars (carbohydrates) or lipids as an energy source. (justia.com)

Numerous enzyme activities are associated with the microsomal fraction. (wakehealth.edu)

Within the rat brain, phosphoramidon-inhibitable conversion of bET-1 to ET-1 was detected principally in the cytoskeletal fraction, i.e., that fraction from the membrane that was not solubilized by detergent treatment. (aspetjournals.org)

Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)
8. Melchers, F.: Biosynthesis, transport and secretion of immunoglobulin in plasma cells. (uni-mainz.de)
11. Melchers, F.: Synthesis, transport and secretion of immunoglobulin in lymphoid cells. (uni-mainz.de)

In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)
In endothelial and epithelial cells the tight junctional complex safeguards barrier function. (meduniwien.ac.at)
Characterization of endothelin-converting enzyme from endothelial cells and rat brain: detection of the formation of biologically active endothelin-1 by rapid bioassay. (aspetjournals.org)

After a semester at Texas Tech University, USA where I studied atmospheric and geoscience, I worked as upper-secondary teacher for three years before turning back to the university in 2002 to get a master degree in Biophysics, thereby turning from large systems i.e. universe and the atmosphere, towards systems on the micro-nano scale i.e. cells and subcellular structures. (lu.se)
My research interest falls into the broad field of regenerative medicine, with emphasis on the way nerve cells and other cell types are affected by small, typically submicron artificial structures. (lu.se)
Although many biomedical applications may come from these studies, the basic science behind this fascinating interaction between cells and manmade structures is the foundation of my research. (lu.se)

Both GIMAP4 and GIMAP7 from human Jurkat cells [ 3 ] localize to the endoplasmic reticulum and Golgi apparatus while mouse Gimap3 from murine IL-3-dependent 32D myeloid precursor cells was expressed at the outer mitochondrial membrane [ 15 ]. (hindawi.com)
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. (wakehealth.edu)

Oxidative damage in microbial cells occurs during exposure to the toxic metal chromium, but it is not certain whether such oxidation accounts for the toxicity of Cr. (microbiologyresearch.org)
To help identify the macromolecular target(s) of Cr-dependent oxidative damage, cells deficient for the reduction of phospholipid hydroperoxides ( gpx3 Δ and gpx1 Δ/ gpx2 Δ/ gpx3 Δ) and for the repair of DNA oxidation ( ogg1 Δ and rad30 Δ/ ogg1 Δ) were tested, but were found not to be Cr-sensitive. (microbiologyresearch.org)

The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)

This enzyme degrades enkephalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. (wikipedia.org)
The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
Using the fluorogenic substrate succinyl-Ala-Ala-Ala-4-methylcoumaryl-7-amide (Suc-Ala-Ala-Ala-MCA), containing the sequence similar to the cleavage sites for proTGF-alpha processing, we identified a putative candidate proTGF-alpha-converting enzyme in the membrane fractions of Chinese hamster ovary (CHO) cells. (nih.gov)
The enzyme activity was about 5-6-fold increased by exposure of the cells with phorbol ester or with calcium ionophore. (nih.gov)
The enzyme was partially purified from membranes of phorbol ester-treated CHO cells. (nih.gov)
Inhibitor profile of the enzyme, including inhibition by serine protease inhibitors and no inhibition by elastatinal, coincides with that previously reported for proTGF-alpha cleavage activity on CHO cells. (nih.gov)

NPEPPS is also reported to play a role in creating and destroying MHC class I-presented peptides and in limiting MHC class I Ag presentation in dendritic cells. (wikipedia.org)

Diabetes causes retinal microvasculopathy associated with pericyte cell death, microaneurysms, abnormal vascular permeability, and macular edema. (molvis.org)

To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
Studies with cells expressing a Sod1p variant (Sod1 H46C ) showed that the superoxide dismutase activity rather than the metal-binding function of Sod1p was required for Cr resistance. (microbiologyresearch.org)
In contrast, S. cerevisiae msra Δ ( mxr1 Δ) and msrb Δ ( ycl033c Δ) mutants defective for peptide methionine sulfoxide reductase (MSR) activity exhibited a Cr sensitivity phenotype, and cells overexpressing these enzymes were Cr-resistant. (microbiologyresearch.org)
hence, the activity probably locates on the outer surface of the cell membrane. (nih.gov)
We loaded gold-quercetin into poly(DL-lactide-co-glycolide) nanoparticles (NQ) and tested the biological activity of NQ on HepG2 hepatocarcinoma cells to acquire the sustained release property. (molcells.org)

Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
Surface functionalization of gold nanoparticles (AuNPs) is essential for biomedical applications that target them to specific disease areas and allow them to selectively interact with cells or biomolecules. (molcells.org)

In 2008 I finished my PhD with the thesis "Controlled axonal outgrowth and cell reactions to nanostructures" and in 2009 I was appointed a position as Assistant Senior Lecturer. (lu.se)

Anatomy51

Organisms12

Diseases1

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment38

Phenomena and Processes39

Disciplines and Occupations5

Technology, Industry, Agriculture2

Information Science3

Health Care2

Activities in subcellular fractions1

Proteins16

Localization5

Tissues12

Mitochondria11

Proliferation7

Bovine aortic1

Recombinant3

Soluble5

Liver7

Lysate1

Metabolism2

Mammalian cells3

Mitochondrial fraction2

Biospecimens1

Induces1

Headings1

Abundance4

Cytosolic2

Biol6

Organisms2

Nucleus1

Myeloma1

Organelles3

Anatomy2

Apoptosis3

Biochemistry2

Membrane-bound1

Lysates3

Tissue2

Hepatic1

Patterns1

Mitotic cell cycle1

Cytokines1

HeLa2

Vitro3

Biology2

Concentration2

Microsomal1

Cytoskeletal1

Secretion3

Endothelial3

Structures3

Endoplasmic reticulum2

Oxidative damage2

Protein function1

Enzyme6

Dendritic1

Abnormal1

Activity5

Selectively2

Reactions1