Cell spliceosome. Medical search. Frequent questions

This approach reveals many previously undetected BAP1 and spliceosome mutations, and uncovers strong evidence that the canonical genomic aberrations in UM usually arise in an early, punctuated burst followed by clonal stasis. (nature.com)
We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
While in agreement with previous studies, little or no evidence for clonal ( SF3B1 mutation) involvement could be found in mature B cells, consistent involvement at the pro-B-cell progenitor stage was established, providing definitive evidence for SF3B1 mutations targeting lymphomyeloid HSCs and compatible with mutated SF3B1 negatively affecting lymphoid development. (nih.gov)
Studies conducted on PRPF31 mRNA levels in lymphoblast cell lines isolated from family members with either deletion or splice site mutations indicate that penetrance requires the co-inheritance of a low expressing wild-type (WT) allele alongside the mutant allele [ 13 , 14 ], whereas the presence of a high expressing WT allele is protective. (molvis.org)
However, mutations in several genes involved in spliceosome formation or function have been shown to cause other conditions with abnormalities affecting the head and face (craniofacial malformations), so craniofacial development is thought to be particularly sensitive to spliceosome problems. (medlineplus.gov)
which means both copies of the gene in each cell have mutations. (medlineplus.gov)
Mutations in minor spliceosome components such as U12 snRNA (cerebellar ataxia) and U4atac snRNA (microcephalic osteodysplastic primordial dwarfism type 1 (MOPD1)) result in tissue-specific symptoms. (biomedcentral.com)
Several years ago, spliceosome mutations in the SF3B1 gene were found to be present in 75-80% of all patients with sideroblastic anemia. (keyence.com)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)
Impact of single versus multiple spliceosome mutations on myelodysplastic syndrome. (cdc.gov)

SMN1 has been linked to pre-mRNA splicing, spliceosome biogenesis, and the nucleolar protein fibrillarin. (medscape.com)
We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
Splicing is a complex, multistep process involving hundreds of proteins: some are responsible for recognising the splice sites, while others stabilise the binding of the spliceosome to the mRNA or cut out the introns and join the exons. (imb.de)
Spliceosomes help process messenger RNA (mRNA), which is a chemical cousin of DNA that serves as a genetic blueprint for making proteins. (medlineplus.gov)
Spliceosomes recognize and then remove regions called introns from immature mRNA molecules to help produce mature mRNA. (medlineplus.gov)
Research suggests that reduced quantities of this spliceosome subunit affect the assembly of the major spliceosome and change the production of a particular group of mRNA molecules. (medlineplus.gov)
Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
Consequently, NUAK1 inhibition in MYC-transformed cells induces global accumulation of RNAPII both at the pause site and at the first exon-intron boundary but does not increase mRNA synthesis. (mdc-berlin.de)
The first step of this process is transcription, through which a piece of DNA in the cell's nucleus is read and copied into a molecule (called mRNA) that can leave the nucleus and travel to the rest of the cell. (uvm.edu)

The minor spliceosome is a ribonucleoprotein complex that catalyses the removal (splicing) of an atypical class of spliceosomal introns (U12-type) from messenger RNAs in some clades of eukaryotes. (wikipedia.org)
U12-type introns represent less than 1% of all introns in human cells. (wikipedia.org)
Additionally, FUBP1 helps promote pairing of splice sites across introns, thereby encouraging spliceosome formation. (imb.de)
It seemed weird and wasteful that introns were being cut out of transcripts by the spliceosome. (evolutionnews.org)
The spliceosome has to recognize the boundaries between introns and exons in the target RNA and remove the right sequence. (mdc-berlin.de)
Here we show that minor intron splicing and AS across minor introns is a highly organised process that might be regulated in coordination with the major spliceosome in a tissue-specific manner. (biomedcentral.com)
In these minor intron-containing genes (MIGs), the majority of introns are usually spliced by the major spliceosome, with only one or two introns being spliced by the minor spliceosome. (biomedcentral.com)
Regardless, mis-splicing of these few minor introns can have detrimental consequences, as highlighted by diseases caused by mutation in minor spliceosome components. (biomedcentral.com)

The minor spliceosome consists of U11, U12, U4atac, and U6atac, together with U5 and an unknown number of non-snRNP proteins. (wikipedia.org)
citation needed] However, a single paper has claimed that the minor spliceosome is active in the cytosol. (wikipedia.org)
Like the major spliceosome, the minor spliceosome had an early origin: several of its characteristic constituents are present in representative organisms from all eukaryotic supergroups for which there is any substantial genome sequence information. (wikipedia.org)
Given that the minor spliceosome is ubiquitously expressed, we hypothesized that these restricted phenotypes might be caused by the tissue-specific regulation of the minor spliceosome targets, i.e. minor intron-containing genes (MIGs). (biomedcentral.com)
We have provided a framework to further study the impact of the minor spliceosome and the regulation of MIG expression. (biomedcentral.com)
These findings may shed light on the mechanism underlying tissue-specific phenotypes in diseases associated with minor spliceosome inactivation. (biomedcentral.com)
Since U4atac snRNA is ubiquitously expressed, mutation in this gene is expected to result in systemic inhibition of the minor spliceosome. (biomedcentral.com)
One way to explain this paradox of systemic minor spliceosome inhibition resulting in tissue-specific phenotypes would be that the MIGs are expressed, spliced and/or alternatively spliced in a tissue-specific manner. (biomedcentral.com)
As a consequence, certain tissues might be more susceptible to loss of minor spliceosome function. (biomedcentral.com)
Indeed, there is evidence that cell-types are differentially affected by loss of minor spliceosome. (biomedcentral.com)

First, FUBP1 stabilises the binding of other splicing proteins to the 3' splice site, helping the cell to recognise the correct exon-intron boundary. (imb.de)
The process of alternative splicing, which had first been observed 26 years before the Human Genome Project was finished, allows a cell to generate different RNAs, and ultimately different proteins, from the same gene . (evolutionnews.org)
Additionally, splicing reactions require the energetically expensive and complex machinery of the spliceosome, comprised of over 150 proteins and 5 snRNA units (Wahl et al. (bartleby.com)
This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
Answering such questions in the cell requires understanding how proteins fit together into a molecular machine such as the spliceosome and how and where they dock onto targets such as RNAs. (mdc-berlin.de)
Using RNAi screening in Drosophila cells, we identified many hnRNPs, SR proteins, core spliceosome, and transcription termination factors that control the outputs of reporter and endogenous genes. (bcm.edu)
Friedman has spent more than five years developing specialized light microscopes to watch single protein molecules, while Hoskins has been developing the methodology to study these proteins in the complex environments necessary for spliceosome function. (nanowerk.com)
RNA contains the chemical information that tells the cells what proteins to make -- for instance, muscle cells use the genes that tell the cell how to make the proteins that are important for muscle, and blood cells use the genes that tell the cell how to make proteins that are important for blood cells. (nanowerk.com)
Here we identified Nup2 in a pool of enriched proteins that co-purify with tagged Ndj1 from meiotic cell extracts. (biorxiv.org)
This essential process creates proteins to sustain advanced organisms, including human life, and the splicing is carried out by a cellular micro-machine called the spliceosome (see http://bit.ly/kokDPM ). (laserfocusworld.com)
Using an unbiased, SILAC MS-based proteomics assay, the major protein targets that are hyper-SUMOylated in cells treated with hinokiflavone were shown to include six proteins that are all components of the U2 snRNP spliceosome subunit that is required for A complex formation. (dundee.ac.uk)
This work was the first to show that three 'normal functions' of the cell - translation by ribosomes, splicing by spliceosomes, and trafficking of proteins around the cell - are really thrown into chaos by the virus. (uvm.edu)
The rest of the cell contains the machinery that creates the proteins (such as interferon) that carry out these instructions. (uvm.edu)
Some proteins are designed to move outside the cell of origin to transmit messages to other cells, like a warning about the presence of a viral infection. (uvm.edu)
it works like a transport system that helps proteins move from inside to outside a cell. (uvm.edu)
This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)

This correlation indicates that linked genes are likely part of a shared regulatory mechanism in the cell. (frontiersin.org)
These cells express two inducible reporter genes , allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively. (invivogen.com)
In addition, these cells feature stable knockout of either the RIG-I (Retinoic Acid Inducible protein 1), MDA5 (Melanoma Differentiation Associated gene 5), or MAVS (Mitochondrial antiviral-signaling protein) genes. (invivogen.com)
Such disruption of splicing can promote cancer growth by upregulating genes that increase cell survival, growth and resistance to anticancer treatments, as well as downregulating genes that promote apoptosis. (imb.de)
It could induce global chromatin change and affects transcription of 6,000 genes in the cell line we studied. (evolutionnews.org)
An intriguing aspect to the association of these genes with retinal disease is that splicing occurs in every cell of the body so the genes must have a general housekeeping function, yet the disease pathology is restricted to the rod photoreceptors of the retina. (molvis.org)
However, it remains unclear what features of these genes dictate dynamic changes in their recognition and stepwise processing by spliceosome components to regulate splice site choice and splicing outcome. (biomedcentral.com)
In this review, we showcase recent studies on the splicing and AS of key genes in metabolic pathways in the liver, the effect of metabolic signals on the spliceosome, and therapeutic intervention points based on RNA splicing. (imperial.ac.uk)
The HOX family of genes plays a major role in embryogenesis and cell differentiation, including differentiation of hematopoietic cell lines. (medscape.com)

The core spliceosome component PRPF8 is essential for spliceosome assembly through its participation in ribonucleoprotein (RNP) complexes for splice-site recognition, branch-point formation and catalysis. (biomedcentral.com)
The team began by investigating how the levels of non-mutated SF3B1, a core spliceosome component, contribute to the MDS disease. (lu.se)

Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)

Furthermore, disruption of this site affects Sm spliceosome activity, leading to intron retention. (rcsb.org)
This process is often disrupted in cancer cells, resulting in exon skipping and/or intron inclusion. (imb.de)
iCLIP of spliceosome components reveals that PRPF8 depletion decreases RNP complex formation at most splice sites in exon-intron junctions throughout the genome. (biomedcentral.com)
We propose that this is because cross-exon interactions are not easily replaced with cross-intron interactions, thereby causing spliceosomes to preferentially assemble across an exon and generate a circular RNA. (bcm.edu)

Localized Inhibition of Protein Phosphatase 1 by NUAK1 Promotes Spliceosome Activity and Reveals a MYC-Sensitive Feedback Control of Transcription. (rndsystems.com)

The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)

Merkel Cell Carcinoma Sensitivity to EZH2 Inhibition Is Mediated by SIX1 Derepression. (harvard.edu)
Splicing factor deficits render hematopoietic stem and progenitor cells sensitive to STAT3 inhibition. (stjude.org)
Inhibition of NUAK1 abolishes chromatin association of PNUTS, reduces spliceosome activity, and suppresses nascent RNA synthesis. (mdc-berlin.de)
Activation of MYC does not bypass the requirement for NUAK1 for spliceosome activity but significantly attenuates transcription inhibition. (mdc-berlin.de)
We suggest that NUAK1 inhibition in the presence of deregulated MYC traps non-productive RNAPII because of the absence of correctly assembled spliceosomes. (mdc-berlin.de)

The TXNL4A gene provides instructions for making one part (subunit) of a protein complex called the major spliceosome, which is the larger of two types of spliceosomes found in human cells. (medlineplus.gov)

Taken together, the data support a model whereby CBC promotes premRNA splicing through a network of interactions with and among spliceosomal snRNPs during cotranscriptional spliceosome assembly. (iucc.ac.il)
Furthermore, it can lead to the design of new and improved anticancer treatments to target disrupted splicing in cancer cells. (imb.de)
Studies show that up to 30% of cancers have more alternative splicing than normal cells. (imb.de)
To better understand how mRNAs are spliced in normal cells and how this is disrupted in cancer, Julian and his colleagues Katja and Michael set out to study FUBP1 (far upstream binding protein 1), which is known to be frequently mutated in gliomas, and to determine whether it has a role in splicing. (imb.de)
Blocks spliceosome-mediated splicing process at the pre-spliceosome/A complex stage. (tocris.com)
One of the most important "doctors" that operates on RNA transcripts is the spliceosome, says a review article in The Scientist about alternative splicing. (evolutionnews.org)
Splicing activity was assayed using an in vivo assay in transfected mammalian cells with rhodopsin ( RHO ) and transducin ( GNAT1 ) splicing templates. (molvis.org)
Together, these findings indicate that lncRNA ABHD11-AS1 interacts with SART3 and regulates CD44 RNA alternative splicing to promote cell malignant transformation and lung carcinogenesis. (cdc.gov)
Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
This process of splicing is carried out by a cellular micro-machine called the spliceosome. (nanowerk.com)
Splicing allows you to decompress the genetic information so the cell can read it before a particular protein is made. (nanowerk.com)
To view the spliceosome in action -- how it assembles to actually do the splicing-the single yeast components are tagged with florescent dyes then the sample is placed into the microscope. (nanowerk.com)
The researchers' paper, "Ordered and Dynamic Assembly of Single Spliceosomes," reports on a five-year-long collaboration of three research laboratories with diverse expertise to study the splicing process. (laserfocusworld.com)
This study identifies hinokiflavone as a potential novel cancer therapeutic and points to a role for protein SUMOylation in regulating spliceosome formation and alternative splicing. (dundee.ac.uk)
Until recently, however, it seemed impossible that such a complex process as splicing and the molecular machine that drives it, the spliceosome, could yield its secrets to structural biologists. (chemistryworld.com)
Mechanistically dissect key RNA metabolism changes causing neural cell dysfunction: Our priorities are to understand the cause and consequence of dysregulated splicing events, and to identify intrinsic master regulators driving transcriptome-wide signatures of neurological disease. (ed.ac.uk)
A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)

Stitching is also useful for evaluating in vitro cultures in bone marrow progenitor cells, where for example we study the mitochondria of erythroblasts as the target structure. (keyence.com)

Trends in Cell Biology, 2021. (bu.edu)

Human and mammalian cells have built-in defense mechanisms to deal with viral infections: when viral genetic material is detected, this triggers a cascade of events - including the release of interferon - designed to shut down the infection and notify neighboring cells of the threat. (uvm.edu)

Chromatin immunoprecipitation and live-cell imaging assays revealed decreased cotranscriptional accumulation of U4/U6·U5 snRNPs on active transcription units, consistent with a requirement for CBC in cotranscriptional spliceosome assembly. (iucc.ac.il)
With the methods to study the splicesome now at their fingertips, the Gelles lab is also researching the process by which the RNA copy is made, called transcription, and processes by which cells change their shape and move. (laserfocusworld.com)
Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
Integration of high-resolution promoter profiling assays reveals novel, cell type-specific transcription start sites across 115 human cell and tissue types. (umassmed.edu)

We can determine whether it happens in the same order on each molecule, or if some spliceosomes assemble differently than others. (nanowerk.com)

Inhibits proliferation of MOLT4 cells in vitro . (rndsystems.com)

inhibits major and minor spliceosomes. (tocris.com)
Inhibits tumor cell invasion. (tocris.com)

The job is handled by an assembly of molecules called the spliceosome , which often removes some of the exons as well. (mdc-berlin.de)
Understanding how these micro-machines function inside the cell is important for many reasons," says Aaron A. Hoskins, a post-doctoral fellow who is a visiting scientist at Brandeis and first author of the paper "Ordered and Dynamic Assembly of Single Spliceosomes," which appears in the March 11, 2011 issue of Science . (nanowerk.com)
If we have one component of the spliceosome that has a green dye on it and one that has a red dye on it, then we see a green spot and a red spot coming together on the RNA, we know that we are studying part of that assembly process," says Gelles. (nanowerk.com)
The entire cell can be viewed as a factory that contains an elaborate network of interlocking assembly lines, each of which is composed of a set of large protein machines. (caseyluskin.com)

Our findings exemplify the regulatory potential of changes in the core spliceosome machinery, which may be relevant to slow-onset human genetic diseases linked to PRPF8 deficiency. (biomedcentral.com)
Cells swim using machines, copy themselves with machinery, ingest food with machinery. (caseyluskin.com)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
A virus like SARS-CoV-2 breaks into a human cell and hijacks the cell's resources and machinery to spread. (uvm.edu)

Nat Rev Mol Cell Biol , Art. (mpi-cbg.de)
Nat Cell Biol Oct;24(10):1475-1486, 2022. (stjude.org)

The location of spliceosomal activity for the minor class spliceosome is regarded by most experts to be in the nucleus. (wikipedia.org)

The U11, U12 and U4atac/U6atac snRNPs are functional analogs of the U1, U2 and U4/U6 snRNPs in the major spliceosome. (wikipedia.org)

YAP1 maintains active chromatin state in head and neck squamous cell carcinomas that promotes tumorigenesis through cooperation with BRD4. (rndsystems.com)

It was further determined that up-regulation of ABHD11-AS1 expression plays an important role in chronic Cr(VI) exposure-induced cell malignant transformation and tumorigenesis, and the stemness of human lung cancer cells. (cdc.gov)
As main findings, 23 miRNAs were already identified as being involved in genetic regulation of PCa cell response to RT. (frontiersin.org)
KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
Furthermore, KIN17 is associated with cancer cell proliferation, migration, invasion and cell cycle regulation by regulating pathways including the p38 MAPK, NF‑κB‑Snail and TGF‑β/Smad2 signaling pathways. (spandidos-publications.com)

Prefoldins contribute to maintaining the levels of the spliceosome LSM2-8 complex through Hsp90 in Arabidopsis. (bu.edu)
As a paper in Nature Reviews Molecular Cell Biology states, "Today biology is revealing the importance of 'molecular machines' and of other highly organized molecular structures that carry out the complex physico-chemical processes on which life is based. (caseyluskin.com)
In eukaryotes - that is, all organisms with cells containing nuclei, from amoebae and worms to humans - the same central dogma masks a much more complex process. (chemistryworld.com)
And one structural biologist will always be associated with the complex and elegant mechanism of this molecular machine: Kiyoshi Nagai, who led the spliceosome group at the LMB. (chemistryworld.com)

Since a certain form of MDS in bone marrow is characterized by pronounced iron overload in the mitochondria of erythroblasts (maturing progenitor cells of red blood cells), he is also interested in specific issues relating to iron metabolism. (keyence.com)
My lab integrates traditional and single-nuclei RNA sequencing to elucidate transcriptome-wide changes to RNA metabolism in clinically relevant post-mortem brain tissue and human induced pluripotent stem cell (hiPSC) models of neurological disease including ALS and autism. (ed.ac.uk)
Define RNA metabolism changes in direct context of human neuropathology: Molecules and networks are characterised at region, cell and pseudotemporal resolution. (ed.ac.uk)

We structurally resolve the interface with PRMT5 and show via genetic perturbation that it is required for methylation of adaptor-recruited substrates including the spliceosome, histones, and ribosomal complexes. (rcsb.org)

[ 12 ] is a juvenile multisystem disorder caused by deletions in mitochondrial DNA (mtDNA) and manifested as severe, refractory sideroblastic anemia, neutropenia, vacuolated cells in bone-marrow precursors, exocrine pancreas insufficiency, malabsorption, and growth failure. (medscape.com)

presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease. (genomicglossaries.com)
Our study thus demonstrates the feasibility of performing gene co-expression network analysis from cell-free RNA data and its utility in studying hepatocellular carcinoma. (frontiersin.org)
In the absence of the gene, programmed cell death persists. (medscape.com)
More even lucky to join Mike Blower's lab at MGH in 2016, learning and growing as a scientist using Xenopus and human cells to understand how gene expression is regulated as cells transit through mitosis. (bu.edu)

Transcriptome-wide Interrogation of the Functional Intronome by Spliceosome Profiling. (umassmed.edu)

Kiyoshi Nagai, who died in 2019, studied the spliceosome at Cambridge's LMB. (chemistryworld.com)

Genetic disruption of the PRMT5-substrate adaptor interface impairs growth of MTAP-null tumor cells and is thus a site for development of therapeutic inhibitors of PRMT5. (rcsb.org)
Surprisingly, many of the genetic changes that occur in cancer cells to make them very aggressive also provide certain vulnerabilities that can be exploited once they are understood. (chop.edu)
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (bvsalud.org)

Surprisingly, when spliceosome components were depleted or inhibited pharmacologically, the steady-state levels of circular RNAs increased while expression of their associated linear mRNAs concomitantly decreased. (bcm.edu)

Targeting SYK signaling in myeloid cells protects against liver fibrosis and hepatocarcinogenesis. (harvard.edu)

Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
It was found that the lncRNA ABHD11-AS1 expression levels are up-regulated in chronic Cr(VI) exposure-transformed human bronchial epithelial cells, chronically Cr(VI)-exposed mouse lung tissues, and human lung cancer cells as well. (cdc.gov)
Bioinformatics analysis revealed that ABHD11-AS1 levels are up-regulated in lung adenocarcinomas (LUADs) tissues and associated with worse overall survival of LUAD patients but not in lung squamous cell carcinomas. (cdc.gov)
As a result, cells build versions of a protein with different modules and functions that are specific to certain tissues. (mdc-berlin.de)
The spliceosome is built from different components in various tissues, to handle their particular needs. (mdc-berlin.de)
Neuroblasts are the immature cells that will eventually develop into the adrenal glands and peripheral nerve tissues of a child. (chop.edu)
While the contribution of these mechanisms to cell and tissue identity is widely accepted, their role in physiological and pathological contexts within tissues is just beginning to be appreciated. (imperial.ac.uk)
The main goal of RT consists in delivering a precise dose of radiation in a target volume, such as tumor, promoting the tumor cells eradication with as minimal damage as possible in surrounding normal tissues ( 13 ). (frontiersin.org)

Division of Hematology and Hematopoietic Stem Cell Transplantation Unit, Ospedale Policlinico San Martino, Genoa, Italy. (bvsalud.org)
In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)

The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. (imperial.ac.uk)

One involved "gluing" the protein onto RNA molecules using a method called PAR-CLIP, provided by Markus Landthaler, extracting RBM20 from cells, and identifying the RNAs attached to it by sequencing them. (mdc-berlin.de)
Molecular machines haul cargo from one place in the cell to another along "highways" made of other molecules, while still others act as cables, ropes, and pulleys to hold the cell in shape. (caseyluskin.com)

Mechanistically, it was found that ABHD11-AS1 directly binds SART3 (spliceosome associated factor 3, U4/U6 recycling protein). (cdc.gov)

A major advantage of the BZ fluorescence microscope for this type of preparation is of course the microscope's inverted beam path, which allows easy positioning and evaluation of the cell cultures. (keyence.com)

The main difference between each of these cells is that a eukaryotic cell has a nucleus and a membrane bound section in which the cell holds the main DNA which are building blocks of life. (bartleby.com)
When abundant, these deposits form a ring around the nucleus, and the cells become ring sideroblasts (see the image below). (medscape.com)

In addition, knockdown of KIN17 was found to enhance the sensitivity of tumor cells to chemotherapeutic agents. (spandidos-publications.com)
The above biological processes have important roles in tumorigenesis, cancer development and chemoresistance in tumor cells. (spandidos-publications.com)

Meiosis is a specialized cellular program required to create haploid gametes from diploid parent cells. (biorxiv.org)
Machines turn cellular switches on and off, sometimes killing the cell or causing it to grow. (caseyluskin.com)

Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)

Sideroblastic anemia is a special form of MDS which involves a massive iron overload of the mitochondria in the erythropoiesis progenitor cells. (keyence.com)

Comparable survival outcomes with haploidentical stem cell transplantation and unrelated bone marrow transplantation. (cdc.gov)

Anatomy6

Organisms6

Diseases1

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes46

Information Science3

Health Care2

Mutations12

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Introns8

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Proteins16

Genes9

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Intron4

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Inhibition5

Subunit1

Splicing18

Evaluating in vitro1

20211

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Inhibits2

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20191

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