• Now it is possible to isolate intact exosomes from cell culture media or any body fluid using the flexible and scalable Total Exosome Isolation reagents. (thermofisher.com)
  • Total exosomes enriched from cell culture (by using the Total Exosome Isolation reagents or ultracentrifugation) can be further purified into specific subpopulations by immunomagnetic capture. (thermofisher.com)
  • In this review article we aimed to emphasize the importance of single-cell analyses by presenting tumor heterogeneity and the limitations of conventional investigational approaches, followed by an overview of the whole single-cell analytic workflow from sample isolation to amplification, sequencing and bioinformatic analysis and a review of recent literature regarding the single-cell analysis of colorectal cancers. (por-journal.com)
  • In this review article, we aimed to present contemporary methods and techniques for the sampling, isolation, and analysis of single cells and to give an overview of the current scientific literature about CRC at the single-cell level. (por-journal.com)
  • CTCs are present in the bloodstream at a very low concentration, thus their detection and characterization require highly sensitive and specific methods, which consist of a combination of enrichment (isolation) and detection (identification) strategies ( Table 1 ) and both steps are essential components of the identification process ( 19 ). (amegroups.org)
  • Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
  • Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
  • After isolation, the source and genetic make-up of CTCs are characterized using nucleic acid-based methods, such as quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), or cytometric-based methods, such as flow cytometry and enzyme-linked immunospot assay technology ( 4 ). (iiarjournals.org)
  • Single-cell sequencing of microbial genomes entails technical challenges relating to the various steps of the required workflow: isolation of individual cells, whole-genome amplification, DNA sequencing, and sequence analysis (Fig. 1 ). (biomedcentral.com)
  • These methods permit targeted isolation of a cell from mixed populations in liquid medium, but isolating microbial cells from primary samples such as swabs and biopsies remains challenging, especially from solid tissues requiring homogenization. (biomedcentral.com)
  • Experimental steps include ( upper half ) isolation and lysis of single cells with subsequent amplification of their genomes, followed by ( lower half ) high-throughput sequencing, genome assembly and genome analysis. (biomedcentral.com)
  • in this scenario, careful consideration must be given to the isolation approach, whereas methods that discriminate against certain subpopulations may result in the exclusion of CTCs that carry biological relevance. (oaepublish.com)
  • Immunomagnetic Isolation of HER2-Positive Breast Cancer Cells Using a Microfluidic Device. (acs.org)
  • The Magnetic Separation Rack is designed for quick and easy smallscale isolation of immunocomplexes from chromatin immunoprecipitations (ChIP assays) using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 or ChIPGrade Protein G Magnetic Beads #9006. (casalomer.es)
  • They are utilized in the magnetic separation and isolation of mouse antibodies from serum, cell culture supernatants, ascites, or mouse antibodylabeled components. (casalomer.es)
  • In general, cell isolation plays a major role in a variety of biological and biomedical applications including disease management. (casalomer.es)
  • Thus, these methodologies are extremely useful to obtain large batches of fairly pure EV samples from cell cultures and enable unbiased characterization of EV subpopulations. (biomedcentral.com)
  • Dendritic cells (DC) for cancer immunotherapy protocols are generated most commonly by in vitro differentiation of monocytes with exogenous cytokines (Mo-DC). (nih.gov)
  • In addition, we demonstrate that using hydrogel-based platforms for the targeted delivery of immune cells, such as NK cells, T cells, and dendritic cells (DCs), has remarkable potential in cancer therapy. (bvsalud.org)
  • We have previously reported that dendritic cells (DCs) might be involved in the immune escape mechanisms for SARS-CoV. (biomedcentral.com)
  • To this end, technologies with the requisite sensitivity and reproducibility to detect CTCs in patients with metastatic disease have recently been developed. (wikipedia.org)
  • On the other hand, CTCs are very rare, often present as only a few cells per milliliter of blood, which makes their detection challenging. (wikipedia.org)
  • Cytokeratin-negative CTCs are characterised by the lack of EpCAM or cytokeratins, which may indicate an undifferentiated phenotype (circulating cancer stem cells) or the acquisition of a mesenchymal phenotype (known as epithelial-mesenchymal transition or EMT). (wikipedia.org)
  • Apoptotic CTCs are traditional CTCs that are undergoing apoptosis (programmed cell death). (wikipedia.org)
  • Measuring the ratio of traditional CTC to apoptotic CTCs-from baseline to therapy-provides clues to treatment efficacy in targeting and killing cancer cells. (wikipedia.org)
  • Small CTCs are cytokeratin-positive and CD45-negative, but with sizes and shapes similar to white blood cells. (wikipedia.org)
  • Small CTCs have been implicated in progressive disease and differentiation into small cell carcinomas, which often require a different therapeutic course. (wikipedia.org)
  • The enumeration of circulating tumor cells (CTCs) represents an effective prognostic and predictive biomarker, which is able to monitor efficacy of adjuvant therapies, detect early development of (micro)metastases and at last, assess therapeutic responses of advanced disease earlier than traditional imaging methods. (amegroups.org)
  • In the last decade, the detection of disseminated tumor cells (DTCs) in the bone marrow and circulating tumor cells (CTCs) in the blood have demonstrated to provide useful information for the clinical management of BC by predicting treatment benefit earlier than traditional imaging methods. (amegroups.org)
  • An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
  • CTCs are tumor cells originating from primary sites or metastases that circulate in the patients' bloodstream and are very rarely found in healthy individuals ( 9 , 10 ) ( Figure 1 ). (iiarjournals.org)
  • Ensemble-decision aliquot position (eDAR) is a sensitive and high-throughput method to analyze circulating tumor cells (CTCs) from peripheral blood. (conferencedequebec.org)
  • The ultimate challenge is based on the need for downstream analyses of the isolated CTCs providing cellular and molecular information at the single-cell level which may be more important than simple enumeration. (conferencedequebec.org)
  • CTCs were captured onto a very small area (1 mm2) with a high enrichment ratio.27 Although eDAR was more sensitive than the CellSearch method in detecting CTCs from metastatic breast cancer patients 27 the original version of eDAR still has several factors that may limit its clinical application. (conferencedequebec.org)
  • The use of circulating tumor cells (CTCs) as a "liquid biopsy" to interrogate the molecular profile of single patients is extremely encouraging, as these cells originate from primary tumor and metastatic sites, providing a comprehensive fingerprint of a given tumor at any time during the course of the disease with a simple blood draw [ 7 ] . (oaepublish.com)
  • Treatment of ground beef enrichment samples with 80% B-PER, a commercially available bacterial cell lysis reagent, and 0.5% sodium azide decreased the culturable bacteria in the samples below detectable levels, increased the sensitivity of the ECL detection 4-5 fold, and decreased the assay time by eliminating the need for heat treatment and filtration steps. (usda.gov)
  • This assay can be recommended as an excellent E. coli O157:H7 screening method for use by the food industry. (usda.gov)
  • In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
  • The changes of immune cells and cytokines in vitro and in vivo were detected by flow cytometry and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
  • In order to accelerate Salmonella diagnosis, we used the immunomagnetic separation assay associated with bacteriophage P22 for the rapid detection of the following Salmonella serovars in chicken rinses of drumsticks, artificially contaminated with 5, 10, and 100 CFU/25mL of bacteria: Salmonella enterica subsp. (pvb.com.br)
  • You can analyze subsets from total exosomes enriched from cell culture using Invitrogen CD9 , CD63 , CD81 , or EpCam -specific reagent, or use the Invitrogen Streptavidin reagent in combination with your choice of biotinylated antibody. (thermofisher.com)
  • If you are looking to compare multiple protein samples on the same gel and want to keep exosomal protein complexes intact, you should check out the fast and gentle Dynabeads-based immunoprecipitation method using Invitrogen Protein A or Protein G in combination with your primary antibody of choice. (thermofisher.com)
  • Immunoassay refers to a class of analytical methods based on antigen-antibody-specific interactions. (peertechzpublications.com)
  • Techniques for analysis of single cell genomics, as well as protein expression on a single cell basis provide scientists with the capacity to understand cancer cell populations as a collection of individual cells, rather than as an average of all cells. (amegroups.org)
  • Now, single-cell genomics of microbial cells has become possible in recent years and offers a solution to this limitation. (biomedcentral.com)
  • Otherwise, their morphology, gene expression and genomics are similar to those of other cancer cells. (wikipedia.org)
  • In this work, a number of cell-lysis reagents and biocides were examined as alternatives to the heat treatment step to decrease bacterial viability in enrichment sample as well as to improve the IM-ECL detection of Escherichia coli O157:H7. (usda.gov)
  • We have developed a DC enrichment platform, which uses the CMRF-44 (IgM) or CMRF-56 (IgG) monoclonal antibodies (mAb) to select BDC that express these antigens after a short overnight incubation. (nih.gov)
  • The field of circulating tumor cell (CTC) enrichment has seen many emerging technologies in recent years, which have resulted in the identification and monitoring of clinically relevant, CTC-based biomarkers that can be analyzed routinely without invasive procedures. (oaepublish.com)
  • Kits for positive selection and enrichment of specific cell types using magnetic particles. (casalomer.es)
  • The CliniMACS Cell Selection System, based upon MACS Technology, takes magnetic cell separation one step further, providing a closed, sterile system for the enrichment of specific cell populations that can subsequently be used in clinical research applications. (casalomer.es)
  • The results confirm the utility of the qPCR method without the pre-enrichment step in tetrathionate as a rapid test for Salmonella spp. (pvb.com.br)
  • Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
  • IHs delivering natural killer (NK) cells, T cells, and antigen-presenting cells (APCs) offer a viable method for treating cancer. (bvsalud.org)
  • These may be used to monitor treatment response, as done experimentally by the Epic Sciences method, which identifies nuclear fragmentation or cytoplasmic blebbing associated with apoptosis. (wikipedia.org)
  • An early approach was to identify the presence of free nucleic acids released from cancer cells into the blood by cancer cell apoptosis and necrosis, as well as by active secretion ( 8 , 9 ). (amegroups.org)
  • Characterization Eumycetoma of the result of INA 6 cells to JAK inhibition revealed effects on intracellular signaling pathways, growth, and apoptosis, each occurring within the same relative concentration array of INCB16562. (igfprotein.com)
  • Currently, there is one FDA-approved method for CTC detection, CellSearch, which is used to diagnose breast, colorectal and prostate cancer. (wikipedia.org)
  • The presence of high expressing epithelial cell adhesion molecule (EpCAM high ) circulating tumor cells (CTC) enumerated by CellSearch ® in blood of cancer patients is strongly associated with poor prognosis. (oncotarget.com)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • To improve the detection of E. coli O157:H7 in ground beef, a more rapid and sensitive method was developed for the analysis of E. coli O157:H7 in ground beef using an immunomagnetic separation (IMS)/ electrochemiluminescent (ECL) procedure. (usda.gov)
  • Once the cell has been isolated, the cell envelope is broken using a procedure that is rigorous enough to rupture recalcitrant taxa, but delicate enough to limit chromosomal break-points that will not be covered in the final sequence. (biomedcentral.com)
  • With customizable protocols, RoboSep® can be optimized for any cell separation, allowing automation to become a part of even the most specialized cell separation procedure. (casalomer.es)
  • FTY720 is a novel drug which induces lymphopenia due its ability to sequester T and B cells into peripheral and mesenteric lymph nodes by a procedure involving sphingosine 1 phosphate receptor on lymphocytes. (igfprotein.com)
  • The purpose of this review is to provide a complete update of the various analytical methods for virus detection, such as molecular, immunological, sensor-based and other detection assays, along with the advantages and limitations of these strategies. (frontiersin.org)
  • A simple fluorometric immunological method in combination with a magnetic concentration step was developed for rapid detection of target bacteria with high sensitivity and specificity in less than 2 h without enumeration. (docksci.com)
  • Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • LTα knockout mice used here were designed to avoid the artifact involving TNF gene downregulation in myeloid cells, which occurred in the conventional LTα knockout mice. (rusimmun.ru)
  • Calvin has a strong interest in process technologies having developed and integrated numerous systems during his time at Juno, including cell separation, gene editing, and cell washing technologies. (allo-searchlight.com)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • The concurrent advance in bioinformatic methods, as well as in high throughput technologies for the analysis of gene expression profiles, allowed deepening the knowledge of genome structure and function. (biomedcentral.com)
  • For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
  • Comparisons of the gene expression profiles of the expanded NK cells and their unstimulated or IL-2-stimulated counterparts showed marked differences. (aacrjournals.org)
  • Several molecular platforms have been used to investigate the molecular profile of the disease, from high throughput gene expression analyses down to single cell biological dissection. (oaepublish.com)
  • As Mcl 1 is a documented STAT3 target gene and an important regulator of cell survival, we surmise this effect plays a part in the observed caspase dependent cell death. (igfprotein.com)
  • In contrast, the presence Bax inhibitor of neutralizing antibodies to AAV2 didn't stop local FIX gene transfer and transgene expression following IM injection of AAV2 development human FIX in human subjects with hemophilia B. The use of drugs targeting B cells prior Lymph node to vector delivery to subjects with high titer antibodies to the vector hasn't been tested yet. (igfprotein.com)
  • Exosomes are small (30-150 nm) vesicles containing sophisticated RNA and protein cargo, that are constantly secreted by all cells in vitro and in vivo . (thermofisher.com)
  • In vivo and vitro, Niraparib can also increase the proportion of T cells and combined with PD-L1 blockade could further enhance the effect. (biomedcentral.com)
  • The expanded NK cells were significantly more potent than unstimulated or IL-2-stimulated NK cells against acute myeloid leukemia cells in vitro . (aacrjournals.org)
  • This review provides an overview of the recent advancements in cancer immunotherapy using IHs for delivering NK cells, T cells, APCs, chemoimmunotherapy, radio-immunotherapy, and photothermal-immunotherapy. (bvsalud.org)
  • Infusions of natural killer (NK) cells are an emerging tool for cancer immunotherapy. (aacrjournals.org)
  • The first report of a CTC came in 1869 from Thomas Ashworth, who described the presence of tumor-derived epithelial cells in the blood compartment of a patient with end stage metastatic cancer, and postulated that this circulation of cells may account for the presence of multiple tumor metastases throughout the body of the patient ( 6 ). (amegroups.org)
  • Diabetes is known to impair the number and function of endothelial progenitor cells in the circulation, causing structural and functional alterations in the micro- and macro-vasculature. (biomedcentral.com)
  • Based on review of current literature, this study examined the expression level of 35 genes that are known to be involved in endothelial progenitor cell migration and function in magnetically sorted Lin - /VEGF-R2 + endothelial progenitor cells obtained from the bone marrow of Akita mice in the early stages of diabetes (18 weeks) using RT-PCR and Western blotting. (biomedcentral.com)
  • We observed a significant increase in the number of Lin - /VEGF-R2 + endothelial progenitor cells within the bone marrow in diabetic mice compared with non-diabetic mice. (biomedcentral.com)
  • Two genes, SDF-1 and SELE, were significantly differentially expressed in diabetic Lin - /VEGF-R2 + endothelial progenitor cells and six other genes, CAV1, eNOS, CLDN5, NANOG, OCLN and BDNF, showed very low levels of expression in diabetic Lin - /VEGF-R2 + progenitor cells. (biomedcentral.com)
  • Low SDF-1 expression may contribute to the dysfunctional mobilization of bone marrow Lin - /VEGF-R2 + endothelial progenitor cells, which may contribute to microvascular injury in early diabetes. (biomedcentral.com)
  • BCR-ABL oncogene activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, contributing to abnormal proliferation of clonal cells. (oncotarget.com)
  • From this perspective, the aim of this study was to analyze the expression and activation profile of STP involved in the mechanisms of cell proliferation/quiescence and survival of the progenitor CD34+ cells from chronic phase (CP) CML. (oncotarget.com)
  • In sum, we report that CD34+ cells from CP-CML are characterized by a proteomic and phospho-proteomic profile that promotes quiescence through the inhibition of proliferation and the promotion of survival. (oncotarget.com)
  • This method demonstrates great potential for detecting other biomarkers and in biochemical applications. (biomedcentral.com)
  • The EasySep™ Release Human PE Positive Selection Kit is designed to isolate cells that are labeled with PE-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection. (stemcell.com)
  • Indeed, it can bypass the extracellular matrix and gradually release small molecules or cells into the tumor microenvironment, thereby boosting immune responses against cancer cells. (bvsalud.org)
  • Nowadays, with single-cell approaches, tumor heterogeneity, the tumor microenvironment, and the interplay between tumor cells and their surroundings can be described in unprecedented detail. (por-journal.com)
  • Many tumors have been described to have marked immune cell infiltration. (por-journal.com)
  • Besides, Niraparib activated the cGAS-STING pathway, increasing the levels of cytokines such as CCL5 and CXCL10, which played a vital role in augmenting the infiltration and activation of cytotoxic T cells. (biomedcentral.com)
  • Immunomagnetic capture combined with electrochemiluminescent detection (IM-ECL) is a rapid and sensitive method for the detection of pathogenic microorganisms from food. (usda.gov)
  • This review summarizes the currently available methods employed for the rapid detection of ZIKV and suggests an innovative approach involving the application of a liposome-based hypothesis for the development of new strategies for ZIKV detection and their use as effective biomedicinal tools. (frontiersin.org)
  • Considering the calamity of ZIKV infection, there is an urgent need to develop rapid detection methods for ZIKV along with DENV, which shares common clinical symptoms with ZIKV. (frontiersin.org)
  • The developed in-situ fluorescent immunomagnetic sensor approach offers distinct advantages because it is rapid, highly sensitive, and easy to use and could therefore be potentially used as a pathogen screening tool. (docksci.com)
  • Currently, detection of breast cancer metastasis relies on clinical manifestations of the spread to distant organs, biopsies of affected organs, radiological evaluations, imaging methods and serum tumor markers ( 3 , 4 ). (iiarjournals.org)
  • Although mammographic screening has reduced the mortality rate associated with metastasis as a result of early diagnosis ( 2 ), the methods described above are frequently inept at detecting metastasis at the earliest stage and at accurately predicting the clinical outcome of the disease ( 3 , 4 ). (iiarjournals.org)
  • In clinical patient samples, Niraparib augmented cytotoxic CD8 + T cell proportion and function. (biomedcentral.com)
  • Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
  • We therefore adapted the K562-mb15-41BBL stimulation method to large-scale clinical-grade conditions, generating large numbers of highly cytotoxic NK cells. (aacrjournals.org)
  • The results that we report here provide rationale and practical platform for clinical testing of expanded and activated NK cells for cell therapy of cancer. (aacrjournals.org)
  • Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
  • With Thermo Scientific™ Culti-Loops™ and Thermo Scientific™ Quanti-Cult™, you can count on trustworthy quality control methods and materials to ensure reproducible clinical test results and consistent microbiology testing for pharmaceutical and food products. (labmal.com)
  • Invariably, the development of new mutations, changes in cancer cell populations, and other resistance mechanisms lead to therapeutic resistance. (amegroups.org)
  • Additionally, a deeper understanding of the metastatic cascade in breast cancer will be critical for developing therapeutic interventions to combat breast cancer metastasis. (iiarjournals.org)
  • Adult stem cells remain of particular interest as therapeutic agents for cardiac regeneration in ischemic heart disease [ 1 - 4 ]. (biomedcentral.com)
  • Additionally, our data highlight the potential exploitation of T cell autophagy as a biomarker of disease activity and provide new ground to verify the therapeutic implications of autophagy as an innovative drug target in pSS. (biomedcentral.com)
  • By using this novel instrument, we investigated the role of JAK1/2 signaling in myeloma cell growth, survival, and resistance to therapeutic treatment. (igfprotein.com)
  • The E. coli O157 isolates were identified by standard biochemical methods and serologic tests by using specific O157 antiserum (INPB-ANLIS "Dr. Carlos G. Malbrán") and sent to the Servicio Fisiopatogenia as National Reference Laboratory (NRL) for further characterization. (cdc.gov)
  • For a long time, food analysis has always been based on physical, chemical, physicochemical, and biochemical methods, however, these are techniques that sometimes require a lot of time and have a low degree of precision. (peertechzpublications.com)
  • comprehensive pathogen detection methods will enable implementation of effective strategies to avoid, or when needed intervene in, the distribution of foods that have been contaminated with foodborne pathogens. (docksci.com)
  • Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
  • In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
  • In our study, we investigated the dynamic changes and responses of immune cells and cytokines during Niraparib treatment. (biomedcentral.com)
  • Interestingly, the SARS-CoV infected DCs showed low expression of antiviral cytokines (IFN-α, IFN-β, IFN-γ and IL-12p40), moderate upregulation of proinflammatory cytokines (TNF-α and IL-6) but significant upregulation of inflammatory chemokines (macrophage inflammatory protein (MIP)-1α/CCL3, regulated upon activation, normal T cell expressed and secreted (RANTES)/CCL-5, interferon-inducible protein of 10 kD (IP-10)/CXCL10 and monocyte chemotactic protein (MCP)-1/CCL2. (biomedcentral.com)
  • Both inhibitors show a for JAK2 over JAK1, JAK3, and Tyk2, but their ability to effectively prevent JAK signaling by cytokines such as IL 6 in myeloma cells could be hampered by Topoisomerase their lack of JAK1 action. (igfprotein.com)
  • Injectable hydrogels (IHs) have emerged as versatile platforms for encapsulating and controlling the release of small molecules and cells, drawing significant attention for their potential to enhance antitumor immune responses while inhibiting metastasis and recurrence. (bvsalud.org)
  • Emerging techniques using the analysis of circulating tumor cells show promising results in predicting and identifying the early stages of breast cancer metastasis in patients. (iiarjournals.org)
  • In this review, the current and novel methods for detection of breast cancer metastasis, as well as the mechanisms involved in metastasis and the treatment of breast cancer metastasis, are discussed. (iiarjournals.org)
  • A circulating tumor cell (CTC) is a cell that has shed into the vasculature or lymphatics from a primary tumor and is carried around the body in the blood circulation. (wikipedia.org)
  • Recent developments in liquid biopsy have increasingly focused on the expanding role of the circulating tumor cell (CTC). (amegroups.org)
  • Subsequently, in 1948, Mandel and Métais found cell-free nucleic acid circulating in the human blood ( 7 ). (amegroups.org)
  • Circulating tumor cells in the blood stream. (iiarjournals.org)
  • On average, 0.88 × 10 6 viable CD133 + cells with a mean log 10 depletion of 3.23 ± 0.19 of non-target cells were isolated. (biomedcentral.com)
  • The dissemination of tumor cells from the primary tumor to distant sites through bloodstream cannot be early detected by standard imaging methods. (amegroups.org)
  • Flow cytometry -Immunomagnetic capture of exosomes on the surface of the Dynabeads allow for a clear and defined FFC/SSC detection typically in less than one hour. (thermofisher.com)
  • Several approaches have been developed to isolate single cells by using either serial dilution, microfluidics, flow cytometry, micromanipulation, or encapsulation in droplets [ 1 ]. (biomedcentral.com)
  • Programmed death ligand 1 (PD-L1) expression in human ovarian cancer cells after PARP inhibitors treatment was examined by western blotting (WB) and flow cytometry. (biomedcentral.com)
  • Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)
  • Electron microscopy (EM) -One of the advantages of using Invitrogen Dynabeads magnetic separation technology to pull out pure exosomal sub-populations is that you can directly perform the EM analysis. (thermofisher.com)
  • This method has many potential advantages which were reported in previous studies [ 17 ], such as (1) the amount of proteins immobilized on the particles is consistent in the same batch, which can be used for performing several reactions. (biomedcentral.com)
  • As miniaturized and simplified stem cell-derived 3D organ-like structures, organoids are rapidly emerging as powerful tools for biomedical applications. (bvsalud.org)
  • Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). (oncotarget.com)
  • There are two not mutually exclusive models explaining tumor heterogeneity: the cancer stem cell and the clonal evolution model. (por-journal.com)
  • This differential signaling activation network may be addressed by novel targeted therapies aimed at eradicating CML stem cells. (oncotarget.com)
  • Interestingly, Niraparib enhanced the proportion and function of T cells via activation of the innate immune response pathway. (biomedcentral.com)
  • Although the aberrant activation of the T cell response observed in pSS has partially been explained by the interaction of T lymphocytes both with the ductal epithelium and the infiltrating B cells via costimulatory molecules (i.e. (biomedcentral.com)
  • First, we introduce IHs as a delivery matrix, then summarize their applications for the local delivery of small molecules and immune cells to elicit robust anticancer immune responses. (bvsalud.org)
  • Exosomes have been shown to transport a range of molecules between different cell types. (thermofisher.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Prior to joining Juno, he developed immunomagnetic cell separation products at STEMCELL Technologies and researched viral transduction enhancing molecules while studying Chemical and Biological Engineering at the University of British Columbia in Vancouver, Canada. (allo-searchlight.com)
  • In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. (scirp.org)
  • Instrumental analyses, commonly used in physical-chemical methods, present systematic errors related to the equipment and reagents used, as well as errors in reading, and weighing by the operator, and require several hours before the results are obtained. (peertechzpublications.com)
  • Magnetic Separation Reagents DeadCell Removal Kits Viahance™ Viahance deadcell removal kit enchances the viability ratio of live to dead cells in cell culture through removal of dead cells and cellular debris using magnetic negative selection. (casalomer.es)
  • Some of these cells have antitumoral behavior (NK cells, CD8 + T cells, CD4 + Th1 cells, and APCs), while others can promote tumor progression (CD4 + Th2 cells, regulatory T cells, and tumor-associated macrophages) [ 13 ]. (por-journal.com)
  • CD133 + stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. (biomedcentral.com)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • The aim of this study was to identify early diabetes-related changes in the expression of genes that have been reported to be closely involved in endothelial progenitor cell migration and function. (biomedcentral.com)
  • Magnetic Cell Separation: Principles and Techniques. (casalomer.es)
  • Safe use of protective clothing and equipment requires specific skills developed through training and experience. (cdc.gov)
  • This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
  • Furthermore, it can define the metabolic features and pathogenic potential of specific bacterial cells and can indicate whether they contain phage and plasmids that facilitate horizontal transfer of genes for clinically relevant traits, such as antibiotic resistance. (biomedcentral.com)
  • Allogeneic Cell Therapy forums provide industry-specific environments to challenge traditional thinking around how to build better companies. (allo-searchlight.com)
  • My motivation for this translational approach is further strengthened by the need for gender-specific knowledge to address the "female way" of developing cardiovascular diseases, and the up to eight-fold increase in risk for later-life cardiovascular disease following a preeclamptic pregnancy. (ntnu.edu)
  • This variation has been useful experimentally to identify the structurally heterogeneous populations of receptors produced in neurons and has raised the possibility that selective drugs could be developed to target specific GABAR isoforms. (aspetjournals.org)
  • Cancerous cells are embedded into diverse tissues consisting of cancer-associated fibroblasts, extracellular matrix, vascular and lymphatic networks, and immune cells, among others. (por-journal.com)
  • Our results showed that Niraparib could upregulate PD-L1 expression, but did not impair the function of immune cells, especially CD8 + T cells. (biomedcentral.com)
  • Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
  • Additionally, imaging methods such as bone scintigraphy, liver echography and chest X-ray are to be performed bianually. (iiarjournals.org)
  • MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
  • The entire manufacturing process, the examination of cell number and viability of the final CP as well as the depletion of non-target cells were part of the validation process, which was performed in compliance with EU guidelines for GMP. (biomedcentral.com)
  • Natural killer (NK) cells can kill cancer cells in the absence of prior stimulation and hold considerable potential for cell-based therapies targeting human malignancies ( 1 - 4 ). (aacrjournals.org)
  • Our results showed that CP-CML CD34+ progenitors were characterized by significant lower phosphorylation of proteins involved in the regulation of growth and cell survival, such as tyrosine kinases of the Src family and members of STAT family, and by a significant higher phosphorylation of p53 (Ser15), compared to normal CD34+ cells from healthy donors. (oncotarget.com)
  • Analysis of expression profile on proteins involved in the apoptotic machinery revealed that, in addition, CD34+ cells from CP-CML were characterized by a significant lower expression of catalase and higher expression of HSP27 and FADD. (oncotarget.com)
  • Ricin causes toxicity by inhibiting the formation (synthesis) of proteins in the cells of the exposed individual. (cdc.gov)
  • Autophagy is a lysosome-mediated catabolic process that allows cells to degrade damaged organelles and misfolded proteins, and to recycle nutrients [ 8 ]. (biomedcentral.com)
  • therefore, there is a need to develop alternative methods to accelerate the detection. (pvb.com.br)
  • However, these conventional methods are time-consuming, laborious, and/or lack in sensitivity in part due to the requirement of cultivation steps. (docksci.com)
  • Conventional genome-assembly algorithms often have problems with single-cell data because they assume that chimeras are rare and genome coverage is Poisson distributed. (biomedcentral.com)
  • The concept of heterogeneity has been around for several decades and gained attention in the 1990s when cancer stem cells were identified in acute myeloid leukemia [ 2 ]. (por-journal.com)
  • In-situ fluorescent immunomagnetic multiplex detection of foodborne pathogens in very low numbers. (docksci.com)
  • Furthermore, the method was highly specific in detecting the target pathogens in a multiplex format. (docksci.com)
  • This regulation requires enhanced detection of pathogens in both imported and domestically produced foods coupled with effective intervention methods to achieve food safety. (docksci.com)
  • Past efforts to develop and implement indicators of waterborne pathogens have often given little or no consideration to the role of evolution in the ecology and natural history of waterborne pathogens of public health concern. (nationalacademies.org)
  • To develop new and more effective indicators of waterborne pathogens it is important to better understand how both evolution and ecology interact with the genomes and natural history of waterborne pathogens and their indicators, if different from themselves. (nationalacademies.org)