• The purpose of this study was to investigate cartilage repair of in vitro lesion models using human bone marrow mesenchymal stromal cells (hBMSCs) with different collagen (Col) scaffolds. (medsci.org)
  • Díaz-Prado, S.M. Human Cartilage Engineering in an In Vitro Repair Model Using Collagen Scaffolds and Mesenchymal Stromal Cells. (medsci.org)
  • Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
  • Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). (biomedcentral.com)
  • Thus far, most of the procedures in human trials have involved autologous transfers of adipose stem/stromal cells (ASC). (pulmonologyadvisor.com)
  • Researchers at the Harvard Wyss Institute have developed a biomaterial scaffold that mimics the actions of antigen-presenting cells (APCs) in stimulating T cell growth and survival. (medgadget.com)
  • The researchers designed a biomaterial scaffold that provides pro-survival and pro-growth biological cues to T cells, just like an APC in the body. (medgadget.com)
  • Within her interdisciplinary research laboratory, Dr Willerth and her team investigate how to engineer neural tissue by combining pluripotent stem cells, controlled drug delivery, and biomaterial scaffolds. (stembook.org)
  • In the analysis of CPRP as a scaffold, it was verified that the cells adhered and spread over the biomaterial. (springer.com)
  • Chapter 5 utilizes a series of CG scaffolds that were inspired by elements of distinct components of orthopedic interfaces (cartilage, tendon/ligament, and bone) to examine the role of biomaterial physical properties (relative density, mineral content) on biasing MSC phenotype in the presence of mixed soluble signals to drive osteogenesis or chondrogenesis. (illinois.edu)
  • Tissue engineering and the use of nanofibrous biomaterial scaffolds offer a unique perspective for studying cancer development in vitro. (chalmers.se)
  • Traditional cell delivery methods involve direct seeding and attachment of MSC on biomaterial scaffolds before in vivo transplantation [ 26 ]. (biomedcentral.com)
  • A major challenge with this technique lies in achieving sufficient T cell growth and survival in vitro to obtain a sufficient dose of reactive T cells. (medgadget.com)
  • Since there has been no in vivo application of graphene in neuronal regenerative medicine, we hope that this review can excite further and concentrated investigations on in vivo (and even in vitro ) neural proliferation, stimulation and differentiation of stem cells on biocompatible graphene scaffolds having the potential of degradability for the generation of implantable neuronal networks. (rsc.org)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • Current in vitro models of tumorigenesis are limited by the use of static, two-dimensional (2D) cell culture monolayers that lack the structural architecture necessary for cell-cell interaction and three-dimensional (3D) scaffolds that are too simplistic for studying basic pathological mechanisms. (chalmers.se)
  • In this study, two nanofibrous biomaterials that mimic the structure of the extracellular matrix, bacterial cellulose and electrospun polycaprolactone (PCL)/collagen I, were investigated as potential 3D scaffolds for an in vitro cancer model. (chalmers.se)
  • This initial analysis has demonstrated the potential of electrospun PCL/collagen I scaffolds toward the development of an improved 3D in vitro cancer model. (chalmers.se)
  • MSCs have great therapeutic potential in tissue engineering, they can be expanded in vitro, and combined with scaffolds they can be inserted into wounds to promote healing and tissue replacement. (bvsalud.org)
  • Researchers have explored combinations of biomaterials, cells, and bioactive factors, using them to generate new cartilage and bone tissue in vitro and in vivo . (corning.com)
  • A team of researchers from Massachusetts General Hospital, Florida State University, and University of Massachusetts has developed a new design of microchip that can retrieve microfluidically attached cancer cells for serial in vitro or in vivo analysis by via integrating a 3D hydrogel scaffold into a fluidic device. (cytofluidix.com)
  • The cell-scaffold scaffold substrate was easily retrieved from the device and cancer cells were shown to be viable for in vitro growth promotion assays. (cytofluidix.com)
  • The in vitro results asserted that the modular scaffold enhanced cell-material interactions by almost five times of those observed for the scaffold alone. (kth.se)
  • When using collagen scaffolds, both their capacity to induce tissue regeneration and their biocompatibility are advantageous characteristics to render them apt for tissue engineering. (nih.gov)
  • In this study, equine collagen scaffolds (TissueFleece) were modified by coating fetal bovine serum proteins, before human bladder smooth muscle cells were seeded. (nih.gov)
  • Cell growth was evaluated by WST-1 proliferation assay and improved when using modified collagen scaffolds. (nih.gov)
  • hBMSCs were seeded on different types of collagen scaffolds. (medsci.org)
  • Taking together, the StereoCount method is an efficient approach for direct cell quantification in 3D collagen scaffolds. (ista.ac.at)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Although graphene/stem cell-based tissue engineering has recently emerged and has promisingly and progressively been utilized for developing one of the most effective regenerative nanomedicines, it suffers from low differentiation efficiency, low hybridization after transplantation and lack of appropriate scaffolds required in implantations without any degrading in functionality of the cells. (rsc.org)
  • Among various stem cells, neural stem cells (NSCs) and their neural differentiation on graphene have attracted a lot of interest, because graphene-based neuronal tissue engineering can promisingly realize the regenerative therapy of various incurable neurological diseases/disorders and the fabrication of neuronal networks. (rsc.org)
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • They have a higher odontogenic differentiation capacity compared to other cell groups due to their content of specific progenitor cells. (scielo.br)
  • Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly (lactic acid) substrates. (springer.com)
  • The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)
  • Chapter 4 uses selective modification of our standard CG scaffold to probe the combined influences of structural and biochemical instructive cues to drive human bone marrow-derived mesenchymal stem cell (MSC) differentiation down tenogenic, osteogenic, and chondrogenic lineages respectively. (illinois.edu)
  • 3D scaffolds with coincident gradients of pore anisotropy and mineral content to drive spatially-graded MSC differentiation were fabricated for the first time. (illinois.edu)
  • Also, stem cell therapy accompanied with scaffolds, is a promising treatment in neural tissue engineering to induce neural differentiation in damaged tissue of brain. (alliedacademies.org)
  • We explored how basic properties of neural cells, nuclei polarization, phenotypic differentiation and distribution/migration, were affected by the culture at poly-L-lactic acid (PLLA) fibrous scaffolds, using a multipotent mitogen-expanded human neural progenitor cell (HNPC) line. (diva-portal.org)
  • We demonstrated that the topography of in vivo-resembling PLLA scaffolds significantly influences HNPC behavior, proven by different migration behavior, phenotypic differentiation potential and nuclei polarization. (diva-portal.org)
  • An optimal paper type is found to provide structural, physical, and mechanical cues to enhance the osteogenic differentiation of human adipose-derived stem cells (hADSCs). (neoscate.com)
  • The results showed that the glass transition temperature modulated the mechanical properties of the scaffolds, affecting stem cell proliferation and adipogenic differentiation. (kth.se)
  • Based on printability and cell-scaffolds interaction results, poly (L-lactide-co-trimethylene carbonate) was used to print 3D scaffolds in different soft and pliable designs that promoted adipogenic differentiation. (kth.se)
  • Collagen-PCL Sheath-Core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-Derived Stem Cells. (ncsu.edu)
  • Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. (ncsu.edu)
  • This is the first study to examine the differentiation behavior of hASCs on collagen–PCL sheath–core bicomponent nanofiber scaffolds developed using a co-axial electrospinning technique. (ncsu.edu)
  • We exploit this method to spectroscopically observe chemical changes that occur during the adipogenic differentiation of human-adipose-derived stem cells over a period of 22 days. (ncsu.edu)
  • After 22 days of differentiation, the cells show visible and spectroscopic indications of completed adipogenesis yet still share spectral features common to the progenitor stem cells. (ncsu.edu)
  • DNA, proteins) for enhancing therapeutic applications, and ability to control material and mechanical properties via the electrospinning process - all advantageous parameters that contribute to the promise of utilizing electrospun scaffolds in tissue repair. (asme.org)
  • Cancer cell adhesion and growth were limited on bacterial cellulose, while all cellular processes were enhanced on the electrospun scaffolds. (chalmers.se)
  • This knowledge is useful in future exploration of in vivo-resembling neural cell system using electrospun scaffolds. (diva-portal.org)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • The studies here use collagen-glycosaminoglycan (CG) scaffolds, regulatory compliant analogs of the native ECM that have been applied to the regeneration of dermis, peripheral nerve, and osteochondral tissue. (illinois.edu)
  • Scaffolds for tissue regeneration must present a viable microenvironment for the living cells to adhere, proliferate, and exhibit the necessary tissue function. (asme.org)
  • These stem cells have renowned ability for use in cell therapy and organ regeneration. (asme.org)
  • Such studies will enable us to understand the effects of drugs such as valproic acid on stem cells and will aid in designing scaffolds for applications in nerve regeneration. (asme.org)
  • Biological scaffolding, either alone or in combination with cells for tissue regeneration, has the potential to revolutionize the repair of injured tissues and to replace tissues lost through disease and injury. (genengnews.com)
  • The aim of this study was to compare the potential of human BMSC cultured as 2D monolayers or 3D spheroids encapsulated in constructs of 3D-printed poly-L-lactide-co-trimethylene carbonate scaffolds and modified human platelet lysate hydrogels (PLATMC-HPLG) for bone regeneration. (biomedcentral.com)
  • The combination of inductive scaffold materials with stem cells might optimize the approaches for bone regeneration. (bvsalud.org)
  • Bioengineers developed biocompatible 3D cell scaffolds for future tissue modeling, regeneration, and drug testing at the cellular level. (nanoscribe.com)
  • A bioactive paper scaffold significantly enhances in vivo bone regeneration of hADSCs in a critical-sized calvarial bone defect. (neoscate.com)
  • This knowledge can be used to engineer 3D scaffolds with adequate physio-chemical and mechanical properties along with an appropriate design that augments adipose tissue regeneration. (kth.se)
  • Further evaluation of the molecular mechanisms responsible for this increased osteoinductivity at an early stage in the regeneration process indicated that the CHA+BMP-2/7 enhanced progenitor cell homing at the implantation site, upregulated the key transcriptomic determinants of bone formation, and increased the production of bone extracellular matrix components. (lu.se)
  • 1. Cell Therapy and Regenerative Medicine Unit, Rheumatology Group, Institute of Biomedical Research of A Coruña (INIBIC), University Hospital Complex A Coruña (CHUAC), Galician Health Service (SERGAS), Department of Medicine, Faculty of Health Sciences, University of A Coruña, A Coruña, Spain. (medsci.org)
  • At McGowan Institute's "Symposium on Biologic Scaffolds for Regenerative Medicine" held recently in Boulder, CO, academic and industry scientists discussed topics ranging from peptide-based scaffolds for nerve tissue to tissue engineering via self assembly. (genengnews.com)
  • Fiber-reinforced scaffolds for tissue engineering and regenerative medicine: use of traditional textile substrates to nanofibrous arrays. (ncsu.edu)
  • Scaffold based strategies for regenerative medicine have focused on the use of three dimensional, biocompatible, biodegradable structures to provide an adequate template for ex vivo cell expansion and maturation, native tissue ingrowth, and restoration of the original tissue qualities with respect to the tissue's biochemical constituents, morphology, form, and function. (ncsu.edu)
  • Fiber diameter and tensile properties of the scaffolds with and without valproic acid are also observed. (asme.org)
  • Salt-leaching 3D scaffolds were fabricated from various medical-grade polyesters, and cellular behavior was evaluated by correlating the physical, chemical, and mechanical properties of the scaffolds. (kth.se)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • The field of tissue engineering has also advanced significantly in harnessing the potential of pluripotent stem cells. (stembook.org)
  • Tissue engineering performs the culture of cells on scaffolds, aiming at the restoration of damaged tissue. (springer.com)
  • Biocompatible materials, such as polycaprolactone (PCL), have been used extensively to fabricate scaffolds using electrospinning technique, to study cell compatibility and to evaluate cell functionality for nerve tissue engineering applications. (asme.org)
  • Tissue engineers working at the laboratory for stem cells and tissue engineering at Columbia University's Fu Foundation School of Engineering are applying a biomimetic approach to developing tissue replacements. (genengnews.com)
  • Polymeric scaffolds comprising two size scales of microfibres and submicron fibres can better support 3D cell growth in tissue engineering, making them an important class of healthcare material. (ucl.ac.uk)
  • Combining expertise from academia and industry from the UK and USA, this study employs a new series of high‐yield, low‐cost scaffolds made by shear‐spinning for tissue engineering. (ucl.ac.uk)
  • This work applies high‐yield shear‐spun scaffolds in mammalian tissue engineering and brings practical, affordable applications of multi‐scale scaffolds closer to reality. (ucl.ac.uk)
  • In this context, tissue engineering becomes relevant for the treatment of large bone defects, where cells or growth factors are incorporated into a three-dimensional scaffold to mimic native tissue architecture and function in terms of osteoconduction, osteoinduction and osteointegration. (amrita.edu)
  • Regardless of monolayer or spheroid cell culture, PLATMC-HPLG constructs represent promising scaffolds for bone tissue engineering applications. (biomedcentral.com)
  • The aim of this paper is to provide a review about current and future materials for scaffolds to carry stem cells in tissue engineering in Dentistry, especially for bone tissue repair. (bvsalud.org)
  • Could tissue engineering and 3D cells create functional replacement tissue for joints damaged by osteoarthritis? (corning.com)
  • Pioneering tissue engineering studies used traditional 2D substrates, but in recent years, research has focused on the development of biomimetic 3D scaffolds and cell culture platforms to repair and regenerate osteoarthritis' osteochondral defects, according to Bio-Design and Manufacturing . (corning.com)
  • Osteochondral tissue engineering is the additive technology that 'makes it possible to spatially pattern cells, bioactive factors, and biomaterials in 3D,' Advanced Healthcare Materials asserts. (corning.com)
  • Discover the new BIO INX series with hydrogels and biodegradable bioresins specifically designed for Nanoscribe Photonic Professional systems as promising materials for live cell printing and tissue engineering. (nanoscribe.com)
  • As part of that mission, researchers from the University of Iowa put their efforts into the development of appropriate biomaterials for tissue engineering of retinal cells. (nanoscribe.com)
  • Our study suggests that paper possesses great potential as a bioactive, functional, and cost-effective scaffold platform for stem cell-mediated bone tissue engineering. (neoscate.com)
  • Mesenchymal stem cell medium was used in the control groups. (scielo.br)
  • To determine the effects of the presence of valproic acid (VA), Wharton's jelly mesenchymal stem cells (MSC) are seeded to the two scaffolds. (asme.org)
  • For their bone reconstruction model, the scientists generated anatomically shaped scaffolds in the exact shape of the human temperomandibular joint (TMJ) bone from decellularized trabecular bone using digitized clinical images, seeded with human mesenchymal stem cells, and cultured with interstitial flow of culture medium. (genengnews.com)
  • Mesenchymal Stem Cells (MSCs) can be isolated from several body tissues, including dental tissues. (bvsalud.org)
  • Mesenchymal stem cells (MSC), which are taken from the bone marrow of adults, provide homeostasis and can travel to repair injured tissue. (pulmonologyadvisor.com)
  • Mesenchymal stromal/stem cell (MSC) expansion in conventional monolayer culture on plastic dishes (2D) leads to progressive loss of functionality and thus challenges fundamental studies on the physiology of skeletal progenitors, as well as translational applications for cellular therapy and molecular medicine. (unibas.ch)
  • In vivo studies showed the presence of the fluorescent-labeled transplanted smooth muscle cells until day 3 and thereafter angiogenesis was induced and infiltration of mouse fibroblasts and polymorphonuclear cells were observed. (nih.gov)
  • However, this method may not be optimal for the delivery of cell spheroids where the 3D structure, essential to maximize their in vivo effects, is lost by direct seeding. (biomedcentral.com)
  • These materials need to match the biochemical and biomechanical properties of the in vivo retinal tissue to promote the cell response desired for disease treatment in the body. (nanoscribe.com)
  • Yet, microfluidic captured tumor cells are difficult to retrieve from devices without disrupting the cell's physiological state for downstream functional characterization that requires ex vivo expansion or in vivo xenograft formation. (cytofluidix.com)
  • Stacking the paper scaffolds with osteogenically differentiated hADSCs and human endothelial cells resulted in vascularized bone formation in vivo. (neoscate.com)
  • Studies of lung scaffolds may offer alternatives to patients awaiting transplant by providing transplantable ex vivo lung tissue and thus avoid the rejection of allograft lungs. (pulmonologyadvisor.com)
  • Enhanced Survival with Implantable Scaffolds That Capture Metastatic Breast Cancer Cells In Vivo. (bvsalud.org)
  • The process involves seeding cells onto biocompatible scaffolds that temporarily act as a supporting structure for cells to attach and grow. (asme.org)
  • The flexibility of this process allows for including a wide array of synthetic and natural biocompatible polymers in the scaffold composition, inclusion of bioactive molecules (e.g. (asme.org)
  • Here, we report a paper-based bioactive scaffold platform for stem cell culture and transplantation for bone reconstruction. (neoscate.com)
  • The use of human stem cell-based models, including human brain organoids and cell transplantation, will further the efforts of the scientific community to unravel the complexity of dopamine neurons, driving advancements in stem cell-based treatments in Parkinson´s disease," revealed Alessandro. (lu.se)
  • The objective of this study is to quantify the effects of the addition of valproic acid to PCL nanofiber scaffolds created through the electrospinning process with regards to cell proliferation. (asme.org)
  • This study tests the hypothesis that the presence of valproic acid in PCL nanofiber scaffolds will enhance cell proliferation. (asme.org)
  • PCL nanofiber scaffolds prepared using a conventional electrospinning technique served as controls. (ncsu.edu)
  • They loaded the rods with Interleukin-2, a protein produced by APCs that can enhance T cell survival, and coated them using a lipid layer, to mimic the APC cell membrane. (medgadget.com)
  • The technology can mimic cartilage's natural mechanical properties, and it can be bioprinted to engineer 3D structures with cells along gradients and more complex biological cues. (corning.com)
  • These microscaffolds were designed to mimic the natural photoreceptor cell environment and fabricated with Nanoscribe's Photonic Professional system using Two-Photon Polymerization. (nanoscribe.com)
  • Parkinsons Congress 2019: Derivation of dopaminergic neurons from embryonic stem cells using a silk nanofibrous scaffold- Maryam Nazm Bojnordi, Ebrahimi-Barough S, Vojoudi E and Ghasemi H- University of Medical Sciences, Iran. (alliedacademies.org)
  • These cell-scaffold constructs were further implanted in the dorsal subcutaneous space of athymic mice. (nih.gov)
  • While many types of matrices have been successfully applied in the clinic, fundamental questions remain about how to combine and manipulate cells with scaffolding to generate functional tissue constructs that avoid immunogenicity. (genengnews.com)
  • cell-free constructs served as controls. (biomedcentral.com)
  • abstract = "We demonstrate a novel application of TiO2 photocatalysis for modifying the cell affinity of a scaffold surface in a cell-culture environment. (elsevierpure.com)
  • The biomaterials have improved along with drug delivery systems for promoting the desired stem cell behavior, along with the methods for confirming tissue function. (stembook.org)
  • Human iPS derived progenitors bioengineered into liver organoids using an inverted colloidal crystal poly (ethylene glycol) scaffold. (ca.gov)
  • To fabricate even softer, and more hydrophilic 3D scaffolds, poly (ɛ-caprolactone-co-p-dioxanone) and a unique scaffold design were utilized within the research group. (kth.se)
  • Esprit BTK is a drug-eluting resorbable scaffold consisting of a temporary scaffold backbone of poly(L-lactide) and a strut thickness of 99 μm. (medscape.com)
  • Bioscaffolding materials may originate from multiple animal, tissue, and cell sources, as well as from synthetic polymers. (genengnews.com)
  • The researchers were able to produce cell scaffolds with a resolution of approximately one micrometer from dissolved proteins and polymers. (thekurzweillibrary.com)
  • The same sets of polymers were further used in melt extrusion-based 3D printer and printability was established for the fabrication of customized 3D scaffolds. (kth.se)
  • The attachment of extracellular matrix or serum proteins to their surfaces does further improve these characteristics, mimicking a close to natural cell environment. (nih.gov)
  • Upon irradiation of ultraviolet (UV) light, OTS molecules were decomposed and became permissive to the adhesion of PC12 cells via adsorption of an extracellular matrix protein, collagen. (elsevierpure.com)
  • B cell scaffold protein with ankyrin repeats 1 is a protein that in humans is encoded by the BANK1 gene. (wikipedia.org)
  • The protein encoded by this gene is a B-cell-specific scaffold protein that functions in B-cell receptor-induced calcium mobilization from intracellular stores. (wikipedia.org)
  • Moreover, a negative regulation of PodJ phase separation by the old-cell-pole scaffold protein SpmX was observed. (nature.com)
  • This protein acts as a tumor suppressor, which means that it keeps cells from growing and dividing too fast or in an uncontrolled way. (medlineplus.gov)
  • The menin protein is present in the nucleus of many different types of cells and appears to be active in all stages of development. (medlineplus.gov)
  • As a result of these mutations, one copy of the MEN1 gene in each cell makes no functional protein. (medlineplus.gov)
  • Incorporation of hydroxyapatite (HA) in a collagen scaffold dramatically improves bone morphogenic protein (BMP) sequestration via biophysical interactions with BMP, thereby providing more controlled BMP release compared with pristine collagen. (lu.se)
  • Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
  • Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. (cdc.gov)
  • 5-times higher on bicomponent collagen–PCL sheath–core scaffolds compared to PCL controls, indicating collagen–PCL bicomponent scaffolds promoted greater hASC osteogenesis after two weeks of culture in osteogenic medium. (ncsu.edu)
  • Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
  • Scaffold proteins are known to physically tether client proteins to specific cellular areas, functioning in spatial regulation of biological processes including signaling transduction, cytokinesis, morphogenesis, and ACD. (nature.com)
  • ideally, the scaffold eventually is replaced by biological tissue. (corning.com)
  • Mechanobiology aims to discover how the mechanical environment affects the biological activity of cells and how cells’ ability to sense these mechanical cues is converted into elicited cellular responses. (ncsu.edu)
  • They are reported to be interesting synthons to synthesize various useful scaffolds of interesting biological activities. (iaamonline.org)
  • Several congenital and acquired diseases of the human genito-urinary tract may need, due to lack or destruction of functional tissues, mechanically stable biomaterials as cell carriers for the engineering of these tissues. (nih.gov)
  • Another exciting area is the development of novel bio-inks for 3D printing stem cell-derived tissues. (stembook.org)
  • The ability to make universal induced pluripotent stem cell lines for engineering replacement tissues would be a game changer as it would make it easier to deliver therapies in rapid manner. (stembook.org)
  • My lab has expanded from engineering neural tissue from stem cells to include directly reprogramming somatic cells into neural tissues, recently published in Frontiers (see here ). (stembook.org)
  • We also recently published a set of papers detailing how we can print functional neural tissues from human induced pluripotent stem cells using the RX1 bioprinter from Aspect Biosystems (see here and here ). (stembook.org)
  • In addition, repair or replacement cells or tissues should not provoke immune reactions in the host. (genengnews.com)
  • Stem cells from dental tissues have a real potential in Advanced Therapies. (bvsalud.org)
  • Bioactive, functional scaffolds are required to improve the regenerative potential of stem cells for tissue reconstruction and functional recovery of damaged tissues. (neoscate.com)
  • How do cells within these mechanically active tissues interpret external loads imposed on their extracellular environment, and, how are cell–substrate interactions converted into biochemical signals? (ncsu.edu)
  • Sonoyama W, Yamaza T, Gronthos S, Shi S. Multipotent stem cells in dental pulp. (scielo.br)
  • Wharton's jelly MSC are multipotent adult stem cells present in the umbilical cord and drawn from their matrix [1,2,3]. (asme.org)
  • BTE aims to combine the cellular ( osteogenic cells), extracellular ( osteoconductive scaffolds) and/or molecular elements ( osteoinductive growth factors) required for bone healing [ 2 ]. (biomedcentral.com)
  • The scaffold allowed the researchers to significantly expand T cell numbers in a dish, compared with existing culture methods, and could bring T cell therapies, such as anti-cancer treatments, closer to clinical reality. (medgadget.com)
  • Researchers at the Harvard Wyss Institute took inspiration from another immune cell type, the APC, which stimulates T cells to grow and survive during an immune response. (medgadget.com)
  • Using the scaffolds, the researchers were able to expand T cells from mice and humans much faster than existing culture techniques, and they confirmed that the expanded cells have clinical potential in a mouse lymphoma model. (medgadget.com)
  • Many different osteochondral scaffolds have been developed by researchers, and some are commercially available. (corning.com)
  • For this, the researchers used Nanoscribe's additive manufacturing technology to create biopolymer retinal cell scaffolds that closely resemble actual characteristics of human retinal tissue. (nanoscribe.com)
  • The researchers developed a process for producing biomimetic scaffolds that closely emulate endogenous (within the body) tissue. (thekurzweillibrary.com)
  • Eight researchers from Lund Stem Cell Center at Lund University have been awarded 41.4 million SEK in the latest announcements from the Swedish Research Council. (lu.se)
  • In their latest grant announcements, eight researchers from Lund Stem Cell Center at Lund University were awarded 41.4 million SEK, giving significant funding support to ground-breaking research in the areas of medicine and health, and natural and engineering sciences. (lu.se)
  • Requirements for scaffolding include biocompatibility, hemocompatability, and the use of nontoxic materials that are durable, functional, and able to support cell growth. (genengnews.com)
  • The main approach for this method is the fabrication and use of scaffold materials to act as a surrogate framework and promote cell-seeded populations to develop into a mature and functional tissue. (ncsu.edu)
  • If the second copy of the MEN1 gene is also altered, the cell has no working copies of the gene and does not produce any functional menin. (medlineplus.gov)
  • This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)
  • Also, the structure is more porous than other models and even regular meniscus tissue, which allows new cells to move into it more easily to integrate with damaged tissue. (ptproductsonline.com)
  • Here we demonstrate that 2D MSC expansion can be entirely bypassed by culturing freshly isolated bone marrow nucleated cells within 3D porous scaffolds in a perfusion-based bioreactor system. (unibas.ch)
  • Three model human cancer cell lines (HEK293, A549 and MCF‐7) with stable expression of GFP were cultured in the scaffolds and found to exhibit efficient cell attachment and sustained 3D growth and proliferation for 30 days. (ucl.ac.uk)
  • The paper scaffolds are surface-engineered by an initiated chemical vapor deposition process for serial coating of a waterrepellent and cell-adhesive polymer film, which ensures the long-term stability in cell culture medium and induces efficient cell attachment. (neoscate.com)
  • Structural and morphological characterization of the scaffolds is also carried out. (asme.org)
  • The chemi- appear to interact with the structural elements of the cell, cal analysis was assessed at DATA CHEM Laboratories using plasma- with apparent binding to the cytoskeleton [Porter et al. (cdc.gov)
  • Research scientists at the Fraunhofer Institute for Laser Technology ILT have succeeded in producing hybrid biomimetic matrices that can serve as a basis for scaffold and implant structures on which cells can grow effectively. (thekurzweillibrary.com)
  • The limited capcacity of the central nervous system in repairmen of neuronal population such as dopaminergic neuron cells suggests stem cell therapy for neurogenesis in Parkinson? (alliedacademies.org)
  • As a result of being capable of differentiating into a variety of cell types, it can be presumed that stem cell therapy has an advantage when compared to other tissue repair methods. (bvsalud.org)
  • The funding received from the Swedish Research Council will go towards his research, 'mapping human dopamine neuron diversity at single-cell resolution for improved stem cell therapy in Parkinson's disease. (lu.se)
  • Cells from the native tissue appear to be naturally attracted to the scaffold - they want to move into it," McNulty states. (ptproductsonline.com)
  • Scientists have tried developing scaffolds or structures from various materials, including plastic and textile fibers, to lay a foundation for new cells. (ptproductsonline.com)
  • Matrigel), immortalized cell lines and resultant structures that are difficult to control or scale. (ca.gov)
  • Anti-cancer T cell therapies involve culturing and sometimes modifying patient-specific T cells in a dish before administering them to a cancer patient, where they can attack and kill cancer cells. (medgadget.com)
  • In the right SEM image, T cells (in blue) bind to a section of a completed antigen-presenting cell-mimetic scaffold (in brown), where they are instructed to multiply and are kept alive for future use in T cell therapies. (medgadget.com)
  • This step is important not only to enhance the efficacy of MSC expansion but also to facilitate clinical translation of cell therapies according to current regulations [ 11 ]. (biomedcentral.com)
  • In addition to measuring cell numbers, circulating tumor cells represent a unique window into understanding a patient's tumor status and developing personalized therapies. (cytofluidix.com)
  • Engineering cartilage has typically involved 'encapsulating chondrocytes, or stem cells which can be differentiated along a chondrogenic linage, in a supportive matrix such as a hydrogel or scaffold,' Advanced Healthcare Materials explains. (corning.com)
  • An integrated microfluidic chip design with an embedded 3-D hydrogel scaffold provides a transfer-rable microenvironment for the recovery and longitudinal study of captured circulating tumor cells. (cytofluidix.com)
  • Their paper describes a simple and effective strategy to integrate a 3D hydrogel substrate into a microfluidic device that can be retrieved after the capture of tumor cells. (cytofluidix.com)
  • A dry hydrogel scaffold was inserted into a capture chamber within a fluidic device and then rehydrated to fill the void volume of the capture chamber. (cytofluidix.com)
  • A cell suspension containing a prostate tumor cell line was used to verify that cancer cells would attach to the hydrogel matrix. (cytofluidix.com)
  • Together, these studies present the framework for building instructive biomaterials to regulate stem cell fate in the context of musculoskeletal tissue repair. (illinois.edu)
  • This review outlines many of the main mechanotransduction mechanisms known to date, and describes recent literature examining effects of both external forces and cell–substrate interactions on musculoskeletal cells. (ncsu.edu)
  • Whether via application of external forces and/or cell–substrate interactions, our understanding and regulation of musculoskeletal mechanobiology can benefit by expanding upon traditional models, and shedding new light through novel investigative approaches. (ncsu.edu)
  • Approaches to scaffolding technology that were discussed at the meeting ranged in complexity from tissue repair products consisting of bovine type 1 collagen knee implants to the highly complex such as the use of whole acellular lung matrix (ACM) to support the development of engineered lung tissue from embryonic stem cells. (genengnews.com)
  • Emerging evidence from preclinical and clinical trials has demonstrated the potential of stem cells to repair lung tissue in chronic obstructive pulmonary disease (COPD). (pulmonologyadvisor.com)
  • The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)
  • Scientifically, she highlighted her work on lung decellularization (removing the cells but leaving the scaffold intact), where hers is one of the leading groups in the field. (lu.se)
  • This is exactly what the Wagner lab is working on by creating cell-free lung scaffolds, which should be less immunogenic. (lu.se)
  • Creating artificial human lung scaffolds from computer models (left), to 3D bioprinting (center), Darcy's team created tubes and branches out of decellularized tissue. (lu.se)
  • Stem cells are the building blocks of modern medicine and hold the key to tackling some of the most complex diseases of our time - from lung disease to neurological disorders to cancer. (lu.se)
  • Multiple cancer cell lines were cultured on each scaffold material and monitored for cell viability, proliferation, adhesion, infiltration, and morphology. (chalmers.se)
  • Advances in 3D bioprinting mean that patients can receive individualized scaffolds and implants. (corning.com)
  • DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP). (scielo.br)
  • In this study, we compared the osteoinductive potential of BMP-2 homodimer with a heterodimer of BMP-2/7, both delivered via a collagen-hydroxyapatite (CHA) scaffold delivery system, with the aim to reduce the overall therapeutic BMP doses and the associated side-effects. (lu.se)
  • My PhD was in an area that looked at the tissue and cell responses to biomaterials. (abc.net.au)
  • The superior osteoinductive properties of BMP-2/7 are a consequence of its direct positive effect on progenitor cell homing at the implantation site, which consequently leads to upregulation of cartilage and bone related genes and biochemical markers. (lu.se)
  • The copolymer 3D scaffolds were further combined with knitted mesh and electrospun nanofibers to develop scaffolds with multilayer architecture, modular scaffolds. (kth.se)
  • editors Culture of human stem cells. (scielo.br)
  • We are beginning to collaborate with Dr. Nygaard who runs the UBCH Clinic for Alzheimer's Disease and Related Disorders , and we plan to bioprint neural tissue models using patient derived human induced pluripotent stem cells for drug screening applications. (stembook.org)
  • Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
  • Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
  • The device is anatomically shaped like a human meniscus and is intended, when sutured to the meniscus rim, to act as a tissue scaffold and fill the void left after meniscus loss. (genengnews.com)
  • Evaluation of the biocompatibility of human induced pluripotent stem cells and rat postnatal retinal cells confirmed cell viability on the 3D printed microscaffolds over several days. (nanoscribe.com)
  • Its major benefit is that automated StereoCount could accelerate research using 3D scaffolds focused on drug discovery for a wide variety of human diseases. (ista.ac.at)
  • Generation of "mini" organs-in-a-dish (organoids) from human pluripotent stem cells represents a powerful new approach to study organ development and model disease. (ca.gov)
  • Generation of human organoids from induced pluripotent stem cells (iPSCs) offers exciting possibilities for developmental biology, disease modelling and cell therapy. (ca.gov)
  • In Situ Monitoring of Adipogenesis with Human-Adipose-Derived Stem Cells Using Surface-Enhanced Raman Spectroscopy. (ncsu.edu)
  • Methods capable of nondestructively collecting high-quality, real-time chemical information from living human stem cells are of increasing importance given the escalating relevance of stem cells in therapeutic and regenerative medicines. (ncsu.edu)
  • This report uses 40-nm colloidal gold nanoparticles to create volumes of surface-enhanced Raman scattering (SERS) within living human-adipose-derived adult stem cells enabling molecular information to be monitored. (ncsu.edu)
  • To achieve this, the use of fiber and/or textile substrates have been used as either the underlying skeleton or reinforcing agents with or without three-dimensional matrices to provide scaffolds that exhibit suitable mechanical properties, high cellularity, and better mimicry of the natural tissue organization and its resulting composition. (ncsu.edu)
  • In the pre-division cell stage, the polar localization of two distinct membraneless signaling complexes, particularly the phosphatase PleC and the kinase DivJ, coordinates to modulate the phosphorylation levels of a set of downstream signaling proteins (including the master regulator CtrA) and determinate the cell fate of C. crescentus . (nature.com)
  • An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO 2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsorption of other proteins and cells. (elsevierpure.com)
  • An as-deposited octadecyltrichlorosilane self-assembled monolayer (OTS SAM) on TiO2 was found to be hydrophobic and stably adsorbed serum albumins that blocked subsequent adsorption of other proteins and cells. (elsevierpure.com)
  • This thesis focuses on assessing cell-material interactions between degradable 3D polymer scaffolds with different designs and adipose tissue-derived stem cells. (kth.se)
  • Drug-Receptor Interactions of New Isoxazole Scaffold against Breast and Skin Cancer Cell Lines', Advanced Materials Proceedings , 4(3), pp. 119-121. (iaamonline.org)
  • Intensive study has demonstrated interactions with host-cell signal transduction and possibly eukaryotic transcription. (cdc.gov)
  • In her research she studies how certain inflammatory mediators such as growth factors and prostaglandins may affect cell-matrix interactions and their role in ongoing remodelling processes and regenerative capacity. (lu.se)
  • This process allows the fabrication of specialized model systems for the study of 3-D cell growth. (thekurzweillibrary.com)
  • Cellular reprogramming also has tremendous promise in terms of cell therapy. (stembook.org)
  • By polarizing different cell fate determinants at opposite cell poles, asymmetric cell division that produces distinct daughter cells is an evolutionarily conserved mechanism to generate cellular diversity in both eukaryotes and prokaryotes. (nature.com)
  • In the case of autologous adipose tissue transfers, many procedures harvest cells from the bone marrow via puncture of the iliac crest, which can increase the risk for infection and considerable pain. (pulmonologyadvisor.com)
  • In addition, subcutaneous adipose tissue yields 500 times the amount of cells that bone marrow does. (pulmonologyadvisor.com)
  • The effects of quantum dots (QD) on cell viability have gained increasing interest due to many recent developments utilizing QD for pharmaceutical and biomedical applications. (ncsu.edu)
  • The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
  • After 4 h of cyclic strain, the cells were examined for cell viability, QD uptake, and cytokine production. (ncsu.edu)
  • The results indicate that addition of strain results in an increase in cytokine production and QD uptake, resulting in irritation and a negative impact on cell viability. (ncsu.edu)