Cell responses vitro and cell. Medical search. Frequent questions

Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. (cdc.gov)
In vitro differentiation of small intestinal epithelium that approaches physiologic functionality of the in vivo host may allow for the development of a pathogenesis model for norovirus. (cdc.gov)
The purpose of the present study was to establish in vitro models to predict the in vivo function of P-gp. (nih.gov)
A good correlation was observed between the in vitro flux ratio across the monolayer and in vivo P-gp function for 12 compounds. (nih.gov)
Collectively, the in vitro flux ratio across monolayers of P-gp-expressing cells may be used to predict in vivo P-gp function. (nih.gov)
The extent of ATP-hydrolysis in vitro may also be a useful parameter for in vivo prediction, particularly for eliminating P-gp substrates in high-throughput screening procedures. (nih.gov)
The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
and the physical, chemical, and physiological characteristics of the components of the immune system in vitro, in situ, and in vivo. (wikipedia.org)
We singled out gut transit time in an unprecedented in vitro approach by subjecting faecal microbial communities from six individuals with either short, medium or long in vivo transit times, to three different colonic transit times of 21, 32 and 63 h in the validated human gut in vitro model, SHIME. (biomedcentral.com)
Given the in vivo transit time variation within and between individuals, the personalisation of in vitro transit time based on in vivo data is required to accurately study intra- and inter-individual differences in gut microbiome structure, functionality and interactions with host and environmental modulators. (biomedcentral.com)
Melero-Martin JM , Khan ZA , Picard A , In vivo vasculogenic potential of human blood-derived endothelial progenitor cells . (avma.org)
These in vitro data are relevant with those found in vivo in insulin-resistant patients. (jci.org)
Therefore, understanding the cellular response to changes in soluble cues through their biomaterial microenvironment, such as oxygen concentration, is crucial for engineering materials that can more closely mimic conditions in vivo and that can potentially lead for improving methods for control over cell behavior in tissue engineering. (aiche.org)
The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)
seem to sprout in response to local delivery of exogenous NGF, demonstrating that axon branching can be directed by NGF in vivo . (jneurosci.org)

These microspheres containing rAg85B were assessed for their ability to deliver antigen to macrophages for subsequent processing and presentation to the specific CD4 T-hybridoma cells DB-1. (rti.org)
Treatment with the anti-AREG antibody prevented resolution of DE exposure-induced airway influx of total cells, neutrophils, and macrophages and increased levels of TNF-a, IL-6, and CXCL1. (cdc.gov)
Monocytes and their derivatives, including macrophages and dendritic cells, play diverse roles in the response to fungal pathogens. (frontiersin.org)
Upon entering affected tissues, classical monocytes can further differentiate into macrophages and monocyte-derived dendritic cells (MoDCs). (frontiersin.org)
Macrophages that differentiate from monocytes in the adult bone marrow are distinguished from tissue resident macrophages (e.g., alveolar macrophages, glial cells) that originally derive from fetal yolk sac progenitor cells or monocytes from the fetal liver ( Hoeffel and Ginhoux, 2018 ). (frontiersin.org)
Classical "inflammatory" monocytes can give rise to non-classical "patrolling" monocytes or further differentiate into macrophages or monocyte-derived dendritic cells (MoDCs). (frontiersin.org)
SL is an antigen-dependent process and requires the collaboration of antigen presenting cells (dendritic cells and macrophages), CD4 + T lymphocytes, and different cytokines. (medscape.com)

Changes in density of viable cells after exposure were measured using the MTT assay. (niom.no)
HPMA exposure resulted in reduced SDH activity (MTT assay), altered cell growth pattern and increased cell death in a dose dependent manner in all investigated cell lines. (niom.no)
Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NO release). (bvsalud.org)
BILTHOVEN, Netherlands, Sept. 19, 2023 (GLOBE NEWSWIRE) -- Intravacc, a Leading Contract Development and manufacturing Organisation for translational research and development of preventive and therapeutic vaccines together with MAT services at Sanquin Diagnostic Services, today announced the publication of a scientific article of an in vitro alternative assay to the replace rabbit pyrogen test for vaccines. (kfor.com)
The MAT is an in vitro assay that can replace the rabbit pyrogen test. (kfor.com)
Using this assay, we will be able to specifically determine the effect of our targeted Tregs on suppressing the autoreactive response. (lupusresearch.org)

Studies of viral attachment to cultured gastrointestinal epithelial cells (Caco-2) using recombinant virus-like particles or infectious noroviruses indicate that specific histo-blood group antigens play a key role in the attachment of the virus to the host cells ( 13 - 17 ). (cdc.gov)
In vitro , recombinant agrin promotes the division of cardiomyocytes that are derived from mouse and human induced pluripotent stem cells through a mechanism that involves the disassembly of the dystrophin-glycoprotein complex, and Yap- and ERK-mediated signalling. (nature.com)

To set up and validate the use of the Vitrocell® Spiking System (VSS), a novel gas mixing system generating low doses of VOCs, coupled to a system of exposure in air/liquid interface, to estimate the VOCs impact on the inflammatory response of airways cells. (vitrocell.com)
Collectively, these studies demonstrate that inhibition of AREG reduced, whereas AREG supplementation promoted, the airway inflammatory recovery response following environmental bioaerosol exposure, and AREG enhanced fibroblast function, suggesting that AREG could be targeted in agricultur al workers repetitively exposed to organic dust environments to potentially prevent and/ or reduce disease. (cdc.gov)
The exposure of mesothelial cells to sclerosants decreased the concentration of IL-6 and IL-8 protein. (medscimonit.com)
The exposure of lung fibroblasts to supernatant from mesothelial cell (with or without adenocarcinoma) did not affect collagen secretion. (medscimonit.com)
Excess mortality from lung cancer was reported among workers employed in a US cadmium recovery plant, and a dose-response relationship was demonstrated between estimated cumulative exposure to cadmium and lung cancer risk. (inchem.org)
Preincubation by an 18-h exposure of Hep G2 cells to 10(-7) M insulin aimed at reducing the number of insulin receptors, was followed by a subsequent 24-h stimulation with 10(-9) M insulin. (jci.org)
In this study, we present the characterization of the two transfected cell lines by demonstrating that differential expression of measurable quantities of the EGFP-HIF-1alpha fusion is achieved with prolonged exposure to hypoxia. (aiche.org)
To inves- inflammatory cytokines and the timing of their production support tigate the interplay of proinflammatory and anti-inflammatory cyto- an immunologic response appropriate to the amount of Ag and kines in human granulomatous disease, we evaluated the ability of duration of exposure. (cdc.gov)
Memory cells travel to the primary follicle, where, after exposure to dendritic cells, they differentiate into centroblasts (immunoglobulin class-switch). (medscape.com)

Transcription factor Sp2 promotes TGFB-mediated interstitial cell osteogenic differentiation in bicuspid aortic valves through a SMAD-dependent pathway. (nih.gov)
Activation of mTOR-dependent pathways regulates the specification and differentiation of CD4 + T effector cell subsets. (jci.org)
Low-level laser therapy has been investigated as a possible stimulus for enhancement of proliferation and differentiation of various cell types, but few reports relate undifferentiated mouse pulp cells (OD-21) response to irradiation in in vitro models. (bvsalud.org)

The majority of infected individuals successfully confine the mycobacterial organisms and remain asymptotic unless immune responses are perturbed. (frontiersin.org)
We further show that bergenin induces Th1 immune responses and potently inhibits bacillary growth in a murine model of Mycobacterium tuberculosis infection. (frontiersin.org)
It is now getting clear that the immune responses contribute to the development of many common disorders not traditionally viewed as immunologic, including metabolic, cardiovascular, cancer, and neurodegenerative conditions like Alzheimer's disease. (wikipedia.org)
IFN-I have potent direct antiviral activities, also mediate a multiplicity of immunoregulatory functions, which can either promote or dampen antiviral adaptive immune responses. (mdpi.com)
Monocytes can also present fungal antigens to elicit adaptive immune responses. (frontiersin.org)
My research is focused on understanding HIV host interactions and the impact these have on disease progression, by deciphering mechanisms behind protective and pathogenic immune responses in HIV-1 and HIV-2 infections. (lu.se)

Both mats supported cell adhesion, proliferation, cellular phenotype and spreading. (unipd.it)
Endoglins are glycoproteins that are involved in various physiological processes such as cell proliferation, extracellular matrix synthesis, immune response or angiogenesis. (biomedcentral.com)
Transcription Factor SP2 Regulates Ski-mediated Astrocyte Proliferation In Vitro. (nih.gov)
HEMA reduces cell proliferation and induces apoptosis in vitro. (niom.no)
We hypothesized that, in CBD, failure of IL-10 to modulate the beryllium-specific, cell-mediated immune response would result in persistent, maximal cytokine production and T lymphocyte proliferation, thus contributing to the development of granulomatous lung disease. (cdc.gov)
bronchoalveolar lavage cells from control and CBD subjects to evaluate the beryllium salt-specific production of endogenous IL-10 and the effects of exogenous human rIL-10 (rhIL-10) on HLA expression, on the production of IL-2, IFN- , and TNF- , and on T lymphocyte proliferation. (cdc.gov)
We of both mitogen- and Ag-induced accessory cell-dependent T lym- phocyte proliferation. (cdc.gov)
Irradiation time of group I influenced positively the proliferation and viability of OD-21 cells in late cell culture period. (bvsalud.org)
B-cell disorders are divided into defects of B-cell development/immunoglobulin production ( immunodeficiencies ) and excessive/uncontrolled proliferation ( lymphomas , leukemias ). (medscape.com)
These pathways converge toward the activation of transcription factors, resulting in B-cell activation and proliferation. (medscape.com)

Primary human monocytes were supplemented with either a 0.01mg/ml or 1mg/ml Sceletium extract dose, with or without E. coli LPS stimulation in vitro, for 24h. (nih.gov)
The acute inflammatory response to LPS stimulation was not negatively affected. (nih.gov)
An addition of soluble factors produced by adenocarcinoma altered the inflammatory response of the pleural mesothelial cells after stimulation with sclerosing agents. (medscimonit.com)
Spry1, Spry2, and Spry4 but not Spry3 are induced transcriptionally and limit the duration and intensity mainly of ERK phosphorylation in response to growth factor (GF) stimulation (with the exception of EGF signaling). (springer.com)
Sulphonylurea-sensitive channels and NO-cGMP pathway modulate the heart rate response to vagal nerve stimulation in vitro. (ox.ac.uk)
Our aim was to establish the functional significance of this and to test whether this modulation could interact with stimulation of the NO-cGMP pathway that facilitates the decrease in heart rate (HR) in response to vagal nerve stimulation (VNS). (ox.ac.uk)

Natural-killer (NK) cells are specialized lymphocytes that have cytotoxic properties in addition to their ability to produce cytokines that assist in the orchestration of adaptive immunity. (medscape.com)
[ 1 ] Neutrophils move to the site of invasion by means of chemotaxis, which occurs in response to microbial products, activated complement proteins, and cytokines. (medscape.com)
ance in the timing or production of these cytokines, resulting in IL-10 is a key inhibitory cytokine in the inflammatory response tissue damage and disease. (cdc.gov)

We have created two cell lines transfected to express an EGFP-HIF-1alpha fusion: 4T1 murine mammary carcinoma and C8161 human subcutaneous melanoma cells. (aiche.org)
Immortalized murine pulp cells (OD-21)6 are considered as the major action mechanism of those lasers.11 undifferentiated mesenchymal stem cells derived from fetal Although this mechanism has not been fully clarified, the most molar papillae7. (bvsalud.org)

CDC activated its emergency outbreak response on May 23, 2022, and the outbreak was declared a Public Health Emergency of International Concern on July 23, 2022, by the World Health Organization (WHO),* and a U.S. Public Health Emergency on August 4, 2022, by the U.S. Department of Health and Human Services. (cdc.gov)
Exp Cell Res, 2022 Feb 1. (nih.gov)

The objective of the current study was to explore possible cellular responses to the methacrylate monomer 2-hydroxy-propylmethacrylate (HPMA) in vitro . (niom.no)
Immunology rests on an understanding of the properties of these two biological entities and the cellular response to both. (wikipedia.org)
Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)
The results indicate that PAI-1 synthesis in presence of insulin is markedly increased in down-regulated cells, and that metformin inhibits this effect by acting at the cellular level. (jci.org)
Thus, HIF-1alpha is an ideal marker for correlating cellular response to oxygen concentration in engineered tissues. (aiche.org)
HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)

In LPS-stimulated human monocyte-derived dendritic cells and Langerhans-like cells, the peptides blocked IL-6 secretion, downregulated expression of maturation markers and inhibited dendritic cell migration. (nature.com)
Plasmacytoid dendritic cells (pDCs) are the professional producers of IFN-I in response to many viruses, including all of the herpesviruses tested. (mdpi.com)
MoDCs are also distinct in origin from conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs) that are derived from a common dendritic cell progenitor (CDP). (frontiersin.org)
B and T cells, type 2 dendritic cells, and natural killer (NK) cells share a common ancestor, ie, common lymphoid progenitor (CLP). (medscape.com)

Toxicity evaluation of root canal sealers in vitro. (niom.no)

The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)

Different cell cultures were exposed to different concentrations of HPMA (0-8 mM) for 24 hours. (niom.no)
Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed. (cdc.gov)
This work focuses on the evaluation of the potential use of electrospun poly(epsilon-caprolactone) (PCL) micrometric and/or sub-micrometric fibrous membranes for rat hippocampal astrocyte (HA) and rat cerebro-microvascular endothelial cell (CEC) cultures. (unipd.it)
The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. (biomedcentral.com)
The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. (biomedcentral.com)
Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. (biomedcentral.com)
The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro . (biomedcentral.com)
The study used mesothelial cell (MeT-5A) cultures stimulated with sclerosing agents (talc, doxycycline, iodopovidone, and TGF-β for 24 h) in the presence or absence of supernatants from adenocarcinoma cultures (HCC827). (medscimonit.com)
Further, lung fibroblasts were cultured with and without cell supernatants from previously established cell cultures for 24 h. (medscimonit.com)
To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)

The latter is further divided into humoral (or antibody) and cell-mediated components. (wikipedia.org)
The (antibody) response is defined as the interaction between antibodies and antigens. (wikipedia.org)
Antibodies are specific proteins released from a certain class of immune cells known as B lymphocytes, while antigens are defined as anything that elicits the generation of antibodies (antibody generators). (wikipedia.org)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)
The lower antibody response in parasite positive subjects may be explained by either antibody consumption or lower protection against malaria parasitemia in subjects with low concentrations of antibodies against the Pf155/RESA antigen. (ajtmh.org)
Centroblasts progress to centrocytes with high-affinity antibody production, and then they differentiate further to long-term memory cells and plasmablasts. (medscape.com)

However, we did not observe changes in cell cycle kinetics or apoptosis induction. (geneticsmr.org)

Mesothelial cells are considered to play a pivotal role in the response to different chemical compounds (sclerosants) used for pleurodesis. (medscimonit.com)

Mitochondrial viability, as an indirect measure of cytotoxicity, and cytokine release in response to the treatment intervention were assessed. (nih.gov)
Cell growth and viability were assessed after 7 and 10 days. (bvsalud.org)
001). Cell viability in all the groups was over 80%, except in group IV at day 7. (bvsalud.org)

Our results showed a dose dependent cytotoxic response to HPMA. (niom.no)
We assessed the cytotoxic, cytostatic, and genotoxic effects of pestheic acid in a gastric adenocarcinoma cell line (PG100). (geneticsmr.org)
Innate immunity resides in the skin, mucous membranes, polymorphonuclear (PMN) cells, complement system, and a select group of cells that possess cytotoxic capabilities. (medscape.com)

The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. (biomedcentral.com)

Microbial growth rates and nutrient utilisation efficiency mediate the species-specific gut microbiota response to in vitro transit time variation, which is the main driver of in vitro microbial load, metabolism and community composition. (biomedcentral.com)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)

Antibodies do not directly kill pathogens, but instead, identify antigens as targets for destruction by other immune cells such as phagocytes or NK cells. (wikipedia.org)
Antigens are foreign substances that evoke an immune response. (medscape.com)
Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
The OMV carrier has been optimized to induce a more effective immune response against these newly introduced antigens. (kfor.com)
We investigated the seroreactivity and T cell reactivity against the Plasmodium falciparum antigen Pf155/RESA, different oligopeptides from the 3′ and 5′ repeat regions of the Pf155/RESA antigen, and crude Plasmodium falciparum antigens in 164 adult Liberians. (ajtmh.org)

Angiogenesis involves a finely regulated sequence of morphogenetic changes that mainly affect endothelial cells. (biomedcentral.com)
One of the main proangiogenic factors is VEGF-A [ 4 ] which is one of the five isoforms of VEGF, a homodimeric glycoprotein with mitogenic action of endothelial cells. (biomedcentral.com)
Edwards N , Langford-Smith AWW , Wilkinson FL , Endothelial progenitor cells: new targets for therapeutics for inflammatory conditions with high cardiovascular risk . (avma.org)

T cell proliferative responses to Chlamydia trachomatis antigen in vitro in patients with a history of gynaecological chlamydia infection. (bmj.com)
Different stages of pathogenic infection were investigated: (i) colonization of the mucus layer, and (ii) adhesion to and (iii) invasion of the epithelial cells. (cranberryinstitute.org)
During inflammation or infection, classical monocytes are mobilized from bone marrow reserves in response to chemokines that bind to the CCR2 receptor, such as CCL2 and CCL7 ( Shi and Pamer, 2011 ). (frontiersin.org)
Das D, Sarkar N, Sengupta I, Pal A, Saha D, Bandopadhyay M, Das C, Narayan J, Singh SP, Chakravarty R. Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study. (wjgnet.com)
A primary cell-based in vitro model of the human small intestine reveals host olfactomedin 4 induction in response to Salmonella Typhimurium infection. (uni-wuerzburg.de)
Development of a multicellular in vitro model of the meningeal blood-CSF barrier to study Neisseria meningitidis infection. (uni-wuerzburg.de)
Regulatory T cells are immune cells that in healthy people stop the immune system's response once an infection has cleared and prevent the immune system from attacking the body's own tissues and cells. (lupusresearch.org)
I was previously focused on HIV-1 and HIV-2 dual infection, HIV-2 mono-infection and in vitro characteruization of HIV-2 Protease. (lu.se)

It was also shown that IDPs from 45S5.2B BG could enhance in vitro HUVEC tubule formation and secretion of interleukin 6 (IL6) and the basic fibroblast growth factor (bFGF). (rsc.org)

The aim of this study was to analyze the insulin-mediated response of PAI-1 and lipid synthesis in Hep G2 cells after producing an insulin-resistant state by decreasing insulin receptor numbers. (jci.org)
Then, expression of the T cell receptor (TCR) is achieved through transduction with a custom lentiviral vector. (lupusresearch.org)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
Following B-cell receptor activation, 2 waves of tyrosine kinase phosphorylation occur. (medscape.com)
The B-cell receptor is formed from the noncovalent association between surface IgM or IgD and 2 transmembrane proteins, IgA and immunoglobulin B. The presence of CD22 and CD19/CD21 on the cell surface, playing the role of coreceptorlike molecules, is necessary for the activation of the receptor. (medscape.com)

This in vitro study evaluated the impact of a cranberry concentrate Type M (CTM) on adherent-invasive Escherichia coli (AIEC) LF82, a pathobiont associated with CD. (cranberryinstitute.org)
Altogether, this exploratory in vitro study demonstrates the prebiotic potential of CTM and supports its antipathogenic effects through direct and/or indirect modulation of the gut microbiome. (cranberryinstitute.org)
Hep G2 cells may be a suitable model to study PAI-1 regulation in response to hyperinsulinemia. (jci.org)
Findings from this study will provide a foundation for clinical studies testing a potentially first-in-class therapy for lupus nephritis that uses specialized regulatory T cells to treat and even possibly cure this disease. (lupusresearch.org)
Human Stem Cells and Tissue Responses to Xenogeneic Bone Block: An In Vitro and Histologic Study. (bvsalud.org)
Chronic beryllium disease (CBD) provides a human disorder in which to study the delayed type IV hypersensitivity response to persistent Ag that leads to noncaseating pulmonary granuloma formation. (cdc.gov)
matory response, IL-10 has been shown to inhibit macrophage and Chronic beryllium disease (CBD) provides a human disorder in T lymphocyte cytokine synthesis and MHC class II and B7 ex- which to study the delayed type hypersensitivity response to persistent pression. (cdc.gov)
The aim of this study was to analyze the influence of low-level laser therapy (=660 nm), with three different irradiation times, on the behavior of OD-21 cell line. (bvsalud.org)

THP-1 macrophage-like cells exposed with PLGA-rAg85B microspheres induced the DB-1 cells to produce IL-2 at a level that was two orders of magnitude larger than the response elicited by soluble rAg85B. (rti.org)

Immunological research continues to become more specialized, pursuing non-classical models of immunity and functions of cells, organs and systems not previously associated with the immune system (Yemeserach 2010). (wikipedia.org)
This review will highlight the roles of monocytes in the immune response to some of the major fungi that cause invasive human disease, including Aspergillus, Cryptococcus, Candida, Histoplasma, Blastomyces , and Coccidioides , and discuss potential strategies to manipulate monocyte responses in order to enhance anti-fungal immunity in susceptible hosts. (frontiersin.org)

The cells involved in recognizing the antigen are Lymphocytes. (wikipedia.org)
THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)

In a mouse model of lupus nephritis, Dr. Ooi found that the specialized regulatory T cells can suppress the misguided immune response that leads to lupus nephritis. (lupusresearch.org)

DB-1 cells recognize the Ag85B(97-112) epitope presented in the context of MHC class II and secrete IL-2 as the cytokine marker. (rti.org)
Cytokine assessment indicated that Sceletium possesses mild anti-inflammatory properties, but does not hinder the mounting of an adequate immune response to acute immune challenge. (nih.gov)
AREG intranasal treatment for 3 days during recovery phase reduced repetitive DE-induced airway inflammatory cell influx and cytokine release. (cdc.gov)
Our data demonstrate that beryllium-stimulated bronchoalveolar lavage cells produce IL-10, and the neutralization of endogenous IL-10 does not increase significantly cytokine production, HLA expression, or T lymphocyte pro- liferation. (cdc.gov)

Recently, the first in vitro norovirus cell culture model was reported for a virus that infects mice ( 18 , 19 ). (cdc.gov)
Representative models of differentiated human intestinal epithelium can be established by growing cells in 3 dimensions (3-D) on collagen-I-coated porous microcarrier beads in rotating-wall vessel (RWV) bioreactors that model the physiologic fluid-shear environment in their respective organs ( 21 - 24 ). (cdc.gov)

Angiogenesis consists of the development of new vessels from pre-existing vascular structures, naturally occurring during embryonic growth and in pathological situations, in response to different stimuli including hypoxia, inflammation or tissue injuries [ 1 ]. (biomedcentral.com)
For this purpose, we have developed oxygen-sensing microparticles that are suspendable through the volume of any transparent biomaterial scaffold used in cell culture and tissue engineering. (aiche.org)
Secondary lymphopoiesis (SL) begins when mature B cells enter the extrafollicular area of lymphoid tissue and differentiate into short-lived plasma cells and memory cells after first being stimulated by antigen-presenting cells. (medscape.com)

0.05) with clinical response to primary therapy and also with response to the DST-selected chemotherapy regimen, but were not associated with survival (P = 0.24). (elsevierpure.com)

Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
Asthma results from complex interactions among inflammatory cells, their mediators, airway epithelium and smooth muscle, and the nervous system. (medscape.com)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
There were also significant differences in microbial composition in dust samples and in innate immune cells between the two groups of children. (medscape.com)
Investigating the innate immune response to HIV-1 and HIV-2 in vitro . (lu.se)

Comparing the results with previous studies of other methacrylate monomers indicate that the response is different from all monomers except the structurally similar 2-hydroxy-ethylmethacrylate (HEMA). (niom.no)

The correlation between in vitro and clinical response was not significant for any individual drug or for all drugs considered together, illustrating the poor predictive value of in vitro testing with currently available chemotherapy in NSCLC. (elsevierpure.com)

Both continue their development in the bone marrow through an antigen-independent process called primary lymphopoiesis (PL). Recognized stages of PL are pro-B cell, pre-B cell, immature B cell, and mature B cell. (medscape.com)

Plasminogen activator inhibitor-1 synthesis in the human hepatoma cell line Hep G2. (jci.org)

The skin comprises various cell types providing the potential to react to bacterial pathogenicity factors and can thus initiate inflammatory responses if exposed to these factors 5 . (nature.com)
Autoimmune diseases, like lupus, are caused by the immune system mistakenly mounting pro-inflammatory responses against specific autoantigens. (lupusresearch.org)

Clinical protocols for small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) were devised to prospectively select individualized chemotherapy based on in vitro drug sensitivity testing (DST) of cell lines derived from the patient's SCLC tumor cell lines or the patient's fresh NSCLC tumor. (elsevierpure.com)
In SCLC, the DST-selected chemotherapy was administered either during weeks 13-24 following 12 weeks of etoposide/cisplatin, or at relapse after complete response to etoposide/cisplatin. (elsevierpure.com)
Five patients treated with their DST-selected chemotherapy attained a complete or partial response, compared to 5 of 68 who received an empiric regimen (P = 0.057). (elsevierpure.com)
14%) in the absence of in vitro results to direct chemotherapy (P = 0.73). (elsevierpure.com)

The absence of repair checkpoints that we observed is probably due to the fact that the PG100 cell line lacks the TP53 gene, which is common in gastric cancers. (geneticsmr.org)
Non-XLA is the result of mu heavy-chain gene deficiency that leads to abortive production of IgM and failure of B-cell development. (medscape.com)

Monocytes can develop from either a granulocyte-monocyte progenitor (GMP) or a monocyte-dendritic cell progenitor (MDP). (frontiersin.org)
Common myeloid progenitor (CMP), granulocyte progenitor (GP), monocyte-committed progenitor (MP), common monocyte progenitor (cMoP), common dendritic cell progenitor (CDP), conventional dendritic cell (cDC), plasmacytoid dendritic cell (pDC). (frontiersin.org)

Oxygen diffusion through tissues is a critical factor in maintaining healthy engineered tissues in vitro. (aiche.org)
Tissues and Cells. (lu.se)

Bacteria can release endotoxins like lipopolysaccharide (LPS) or other pathogenicity factors such as lipopoteins/peptides (LP) from their cell envelope, even due to treatment with conventional antibiotics, being able to activate Toll-like receptors (TLRs) and induce a strong inflammatory response. (nature.com)
THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. (sigmaaldrich.com)

The response pattern varied depending on time and type of cell line used for analysis, although the results indicate a higher cytotoxicity for EPH in short-term tests. (bvsalud.org)
Based on our results, the proper fiber architecture can be regarded as a crucial issue to be considered in order to deal with suitable polymeric mats tailored for specific in vitro application. (unipd.it)
These results are consistent with the hypothesis that inhibition of sulphonylurea-sensitive channels can increase the HR response to VNS by a pre-synaptic mechanism, and that this modulation may be independent of activation of the NO-cGMP pathway. (ox.ac.uk)

Anatomy31

Organisms24

Diseases20

Chemicals and Drugs109

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes43

Information Science2

Health Care2

Vivo15

Macrophages7

Assay6

Recombinant2

Exposure9

Differentiation3

Immune responses6

Proliferation10

Stimulation6

Cytokines3

Murine2

20222

Cellular6

Dendritic cells4

Root canal sealers1

Adaptive immune2

Cultures10

Antibody6

Apoptosis1

Chemical Compounds1

Viability3

Cytotoxic3

Angiogenic1

Metabolism2

Antigens5

Endothelial3

Infection8

Secretion1

Receptor5

Study8

Soluble1

Immunity2

Lymphocytes2

Suppress1

Cytokine4

Model2

Tissue3

0.051

Epithelium2

Innate immune3

HEMA1

Correlation1

Bone marrow1

Human hepatoma1

Inflammatory responses2

Chemotherapy4

Gene2

Progenitor2

Tissues2

Receptors2

Results3