• One example is adoptive cell therapy with T cells that were genetically modified with gamma-retroviral and lentiviral (LV) delivery vectors to express a CD19-specific chimeric antigen receptor (CAR) for cancer treatment. (uni-wuerzburg.de)
  • In addition, due to the great diversity of immune cell antigen receptor recombination breakpoints, only ~85% of all clonal T-cell populations will be identified with the primers utilized in this assay. (ohsu.edu)
  • Human T-cell antigen receptor gene T3-delta. (lu.se)
  • Epidermal growth factor receptor in non-small-cell lung carcinomas: correlation between gene copy number and protein expression and impact on prognosis. (nih.gov)
  • The epidermal growth factor receptor (EGFR) is frequently overexpressed in non-small-cell lung carcinoma (NSCLC), and EGFR inhibitors are promising new therapeutic agents. (nih.gov)
  • Activating mutations in downstream genes of the epidermal growth factor receptor (EGFR) pathway may cause anti-EGFR resistance in patients with colorectal cancers. (ecologicalsgardens.com)
  • The gene structure and expression of the non-specific cytotoxic cell receptor protein (NCCRP-1) in Atlantic cod (Gadus morhua L. (nofima.no)
  • Protein expression was assessed by immunohistochemistry on a scale from 0 to 400 (percentage of positive cells x staining intensity). (nih.gov)
  • It will be important to evaluate EGFR gene and EGFR protein status and signal protein expression to properly interpret future clinical trials using EGFR inhibitors. (nih.gov)
  • In the simplest receptors, polypeptide chains cross the lipid bilayer once, while others, such as the G-protein coupled receptors, cross as many as seven times. (wikipedia.org)
  • In some receptors, such as the nicotinic acetylcholine receptor, the transmembrane domain forms a protein pore through the membrane, or around the ion channel. (wikipedia.org)
  • Receptor protein: cells must have cell surface receptor proteins which bind to the signaling molecule and communicate inward into the cell. (wikipedia.org)
  • Binding of the signal molecule to the receptor protein will activate intracellular signaling proteins that initiate a signaling cascade. (wikipedia.org)
  • T-cell receptor (TCR)-induced IL-2 expression is suppressed at the level of mRNA and protein following chronic culture of T cells with tumour necrosis factor (TNF) at picomolar concentrations, an effect that is reversible upon removal of TNF. (biomedcentral.com)
  • Here we demonstrate that it binds Patched (vPtc), which has been identified as a tumour-suppressor protein in basal cell carcinoma, with high affinity. (nih.gov)
  • A collaboration of scientists from Earle A. Chiles Research Institute of the Providence Cancer Institute and the National Cancer Institute previously identified T-cell receptors that target KRAS G12D, a "hot spot" signaling protein mutation found in certain cancers. (cytologicsbio.com)
  • To engineer T cells targeting mutant KRAS protein, T-cell receptors were isolated from the previously treated patient with colorectal cancer, synthesized, and cloned for use to transduce the PBMCs. (cytologicsbio.com)
  • Our approach pairs sparse scATAC-seq data with robust detection of hundreds of cell surface and intracellular protein markers and optional capture of mitochondrial DNA for clonal tracking, capturing three distinct modalities in single cells. (nature.com)
  • ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
  • Furthermore, we analysed the 5'-flanking region of the human adipose differentiation-related protein ( adrp ) gene that responded to all subtypes of PPARs. (biomedcentral.com)
  • Overview of Genetics A gene, the basic unit of heredity, is a segment of DNA containing all the information necessary to synthesize a polypeptide (protein) or a functional RNA molecule. (msdmanuals.com)
  • When analysing separately subgroups, according to the oestrogen receptor (ER) status both approaches could predict clinical outcome more easily for the ER-positive than for the ER-negative cohort. (lu.se)
  • Kunnas and colleagues studied a dinucleotide repeat of the alpha oestrogen receptor gene in 119 Finnish men who died suddenly. (the-scientist.com)
  • The extent of this polymorphism of the alpha oestrogen receptor gene is associated with the. (the-scientist.com)
  • Configuration of immunoglobulin and T cell receptor beta and gamma genes in acute myeloid leukaemia: pitfalls in the analysis of 40 cases. (bmj.com)
  • Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. (ohsu.edu)
  • Rearrangement of the genes for the beta and gamma chains of the T cell receptor is rarely observed in adult B cell lymphoma and chronic lymphocytic leukemia. (jci.org)
  • In 7 of the 100 B cell specimens, a single nongermline band was detected after digestion with the restriction enzyme BamHI, but the rearrangement could be confirmed with a second restriction enzyme in only two. (jci.org)
  • A rearrangement of the T gamma gene was never seen in a B cell sample. (jci.org)
  • In contrast, T cell specimens usually rearranged both alleles of T beta (15 of 18), the rearrangement could be confirmed with a second restriction enzyme (17 of 18), both alleles of the first constant region gene segment of T beta always underwent either rearrangement or deletion, and the T gamma gene was also rearranged or deleted (17 of 18). (jci.org)
  • We conclude that ordered rearrangement of the T cell receptor is a rare event in B cell lymphoma and B-CLL. (jci.org)
  • We studied the configuration and expression of the gene encoding the beta chain of the T cell receptor (TCR beta) in cell lines and primary tumor cells infected by the human T cell leukemia/lymphoma (lymphotrophic) virus type I (HTLV-I). Most of the cell lines and all the primary tumor cells showed rearrangement of the TCR beta gene, and in each case the rearrangement was distinct. (rupress.org)
  • The majority of cases examined were clonal with respect to a particular TCR beta gene rearrangement. (rupress.org)
  • This suggests that the chromosomal abnormality, and possibly infection by HTLV-I, occurred before TCR beta gene rearrangement. (rupress.org)
  • The heterodimeric T-cell surface receptors, either alpha/beta (90% -95% of T cells) or gamma/delta (5% - 10% of T cells) are produced following somatic rearrangement of the T-cell receptor (TCR) genes (alpha, beta, delta, and gamma). (ohsu.edu)
  • In conjunction with morphologic evaluation of lymph nodes, bone marrow and other tissue types the detection of a clonal T-cell gene rearrangement by polymerase chain reaction (PCR) can be used to aid a diagnosis of malignant T-cell lymphoma. (ohsu.edu)
  • Loss of IL-7R function is also associated with the loss of a T-cell receptor (TCR) rearrangement. (medscape.com)
  • In the second part of the study, we ablated expression of PD-1, a checkpoint and negative regulator of T cell function to improve the therapeutic index of CAR T cells. (uni-wuerzburg.de)
  • Configuration and expression of the T cell receptor beta chain gene in human T-lymphotrophic virus I-infected cells. (rupress.org)
  • Levels of expression of the TCR beta gene at the mRNA level and surface expression of the T3 complex were also not significantly altered, suggesting that changes in immune function cannot be attributed to quantitative changes in the TCR molecule. (rupress.org)
  • How does tumour necrosis factor uncouple T-cell receptor-induced IL-2 gene expression in murine T cells? (biomedcentral.com)
  • We have found that, although chronic TNF attenuates TCRζ expression and TCR-proximal signalling, IL-2 expression is suppressed regardless of whether cells are stimulated via the TCR, or with phorbol ester and calcium ionophore to activate the MAPK and NFAT pathways directly. (biomedcentral.com)
  • However, the initial rate of decay was similar in control and TNF-treated cells, suggesting that reduced expression in TNF-treated cells was not due to decreased stability of IL-2 mRNA. (biomedcentral.com)
  • c-Rel, IκBβ and IκBε were regulated differently in control and TNF-treated cells: expression of IκBε was comparatively enhanced, while that of c-Rel and IκBβ was attenuated in TNF-treated cells. (biomedcentral.com)
  • However, altered regulation of NF-κB expression and activity in TNF-treated cells may contribute to poor inducibility of IL-2 through effects on stimulus-induced changes in chromatin conformation across pIL-2. (biomedcentral.com)
  • Isomäki P, Panesar M, Annenkov A, Clark JM, Foxwell BM, Chernajovsky Y, Cope AP: Prolonged exposure of T cells to TNF down-regulates TCR zeta and expression of the TCR/CD3 complex at the cell surface. (biomedcentral.com)
  • Data are available at the Gene Expression Omnibus under accession number GSE156478 . (nature.com)
  • The aim of this thesis was to examine the changes in gene expression during foam cell formation and to study the HDL-receptor scavenger receptor class B type I (SR-BI), which may be of importance for reverse cholesterol transport.To search for genes that may be of importance for foam cell formation, expression profiling of human macrophages exposed to oxLDL was performed. (gu.se)
  • OxLDL increased expression of genes involved in uptake of oxLDL, storage of lipids and cholesterol efflux whereas expression of genes involved in cholesterol synthesis and LDL-uptake was decreased. (gu.se)
  • OxLDL also induced a cellular stress response, with increased expression of genes encoding heat shock proteins, metallothioneins and enzymes in the glutathione and the thioredoxin system. (gu.se)
  • In conclusion, expression profiling of macrophages revealed changes in the expression of several genes and may provide clues to the mechanisms behind foam cell formation. (gu.se)
  • Whole blood cells, aorta and lung were harvested for global gene expression analysis with subsequent Ingenuity Pathway Analysis and confirmatory qRT-PCR. (cdc.gov)
  • In addition, pulmonary expression of interferon alpha and beta and Irf7 specific pattern recognition receptors (PRR) and signaling molecules (Ddx58, Ifih1, Dhx58, ISGF3) were induced, an effect that showed specificity when compared to other inflammatory exposures. (cdc.gov)
  • In vivo, poly(I:C) treatment increased intratumoral chemokine expression, NK-cell activation and tumor infiltration, and proliferation of tumor-infiltrating T and NK cells. (nih.gov)
  • TLR3 expression in patient samples correlated with NK-cell activation, NK- and T-cell tumor infiltration, and inversely correlated with tumor parenchyma cell viability. (nih.gov)
  • We have studied the effects of excitatory amino acids on the expression of the c-fos and c-jun mRNA in rat C6 glioma cells. (kyobobook.co.kr)
  • We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
  • Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
  • We compared the power of gene expression measurements with that of conventional prognostic markers, i.e., clinical, histo- pathological, and cell biological parameters, for predicting distant metastases in breast cancer patients using both established prognostic indices (e.g., the Nottingham Prognostic Index (NPI)) and novel combinations of conventional markers. (lu.se)
  • The gene expression profiler did not perform noticeably better than indices con- structed from the clinical variables, e.g., the well established NPI. (lu.se)
  • In recent exploratory regional therapy alone or by the addition of systemical studies, markers from microarray gene expression medical therapy with increased side effects and costs. (lu.se)
  • These gene expression results have into different risk group in order to help in making been compared with data obtained from using conven- treatment decisions. (lu.se)
  • Although these comparisons favoured the gene expression markers, this was usually in comparison against single factors and the new tech- q Sources of support: Swedish Research Council, Swedish Founda- nology has not been tested against a combined index, tion for Strategic Research, Lund University Medical Faculty and the such as the Nottingham Prognostic Index (NPI). (lu.se)
  • After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
  • GCs, such as for example dexamethasone (dex), induce cell loss of life through the GC receptor (GR), a ligand-activated transcription aspect whose transcriptional activity is necessary for GC cytotoxicity.1 GR regulates gene expression by binding DNA and nucleating the set up of regulatory cofactors. (rawveronica.com)
  • Expression of wild type GR in COR L24 cells restored glucocorticoid signaling, with marked induction of GMCO reporter gene expression by dexamethasone (9,100±910%, n = 3), and an estimated EC50 of 10 nM. (manchester.ac.uk)
  • The study was conducted to establish the differences in the expression pattern of different cell surface toll-like-receptors ( TLRs) between indigenous Osmanabadi and Salem Black goats subjected to summer season induced heat stress. (malque.pub)
  • The results indicated that the expression of TLR1, TLR4, TLR5, TLR6, and TLR10 genes in both the breeds showed different expression pattern. (malque.pub)
  • 0.05) expression of these genes were reported in heat stress group (SBHS) as compared to control group (SBC) in Salem Black breed. (malque.pub)
  • The higher expression of most of the cell surface TLRs in the SBHS group as compared to OHS group indicates the superior resilient capacity of Salem Black goats to maintain immune status even during exposure to adverse environmental condition. (malque.pub)
  • Bharati J, Dangi SS, Mishra SR, Chouhan VS, Verma V, Shankar O, Bharti MK, Paul A, Mahato DK, Rajesh G (2017) Expression analysis of Toll like receptors and interleukins in Tharparkar cattle during acclimation to heat stress exposure. (malque.pub)
  • To identify human PPARs-responsive genes, we established tetracycline-regulated human hepatoblastoma cell lines that can be induced to express each human PPAR and investigated the gene expression profiles of these cells. (biomedcentral.com)
  • The expression of each introduced PPAR gene was investigated using the various concentrations of doxycycline in the culture media. (biomedcentral.com)
  • We found that the expression of each PPAR subtype was tightly controlled by the concentration of doxycycline in these established cell lines. (biomedcentral.com)
  • Interestingly, it was noted that while ligand-activated PPARδ induced target gene expression, unliganded PPARδ repressed these genes. (biomedcentral.com)
  • The real-time RT-PCR was used to verify the altered expression of selected genes by PPARs and we found that these genes were induced to express in the same pattern as detected in the microarray analyses. (biomedcentral.com)
  • (B) qRT-PCR analysis of 11 EBM epithelial cell RNA samples showing high levels of ESR1 expression normalised against ER-pos MCF7 and ER-neg MDA-MB-231 cell lines, (range 2.2- 19-fold higher). (biomedcentral.com)
  • fBB 4 -R expression in cells widely used for other Bn receptor subtypes was unsuccessful as was expression in two frog cell lines. (acs.org)
  • While the percentages of CD56 (dim) cells and their CD16 expression were lower in the chronic group, this was not statistically significant. (who.int)
  • The single-nucleotide polymorphism rs1582763 modified expression of the MS4A4A and MS4A6A genes in multiple tissues, suggesting that one or both of these genes are important for modulating sTREM2 production. (lu.se)
  • This ulcerated nodule of localized primary cutaneous CD30+ anaplastic large cell lymphoma is relatively nonspecific in appearance. (medscape.com)
  • Scholars@Duke publication: Regulation of T cell receptor-alpha gene recombination by transcription. (duke.edu)
  • Tissue distribution and regulation of SR-BI indicate that it may function both as a scavenger receptor and as a receptor for HDL-cholesterol. (gu.se)
  • The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
  • Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors and commonly play an important role in the regulation of lipid homeostasis. (biomedcentral.com)
  • and positive regulation of natural killer cell mediated cytotoxicity. (mcw.edu)
  • Inhibition of phosphatidylinositol 3-kinase (PI3K), a linchpin in the pre-B-cell receptor and interleukin 7 receptor signaling pathways essential to B-cell advancement (with CAL-101 [idelalisib]), interrupts a double-negative opinions loop, improving GC-regulated transcription to synergistically destroy even extremely resistant B-ALL with varied hereditary backgrounds. (rawveronica.com)
  • A novel pathogenic mutation on the interleukin-7 receptor has been described in a newborn. (medscape.com)
  • The advances in genetic engineering have enabled us to confer T cells new desired functions or delete their specific undesired endogenous properties for improving their antitumor function. (uni-wuerzburg.de)
  • One example is adoptive cell therapy with T cells that were genetically modified with gamma-retroviral and lentiviral (LV) delivery vectors to express a CD19-specific chimeric The advances in genetic engineering have enabled us to confer T cells new desired functions or delete their specific undesired endogenous properties for improving their antitumor function. (uni-wuerzburg.de)
  • In conclusion, we provide a novel platform for virus-free genetic engineering of CAR T cells that can be broadly applied in T-cell cancer therapy. (uni-wuerzburg.de)
  • These findings, combined with available genetic evidence from Drosophila, support the hypothesis that Ptc is a receptor for Shh, and that vSmo could be a signalling component that is linked to Ptc. (nih.gov)
  • Explore the normal functions of human genes and the health implications of genetic changes. (medlineplus.gov)
  • Nam, A. S., Chaligne, R. & Landau, D. A. Integrating genetic and non-genetic determinants of cancer evolution by single-cell multi-omics. (nature.com)
  • Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
  • With the advances in BMT and gene therapy, patients now have a better likelihood of developing a functional immune system in a previously lethal genetic disease. (medscape.com)
  • The Online Mendelian Inheritance in Man ( OMIM ) database is a searchable catalog of human genes and genetic disorders. (msdmanuals.com)
  • They are specialized integral membrane proteins that allow communication between the cell and the extracellular space. (wikipedia.org)
  • Many membrane receptors are transmembrane proteins. (wikipedia.org)
  • The rotation alters which parts of the receptor are exposed on the intracellular side of the membrane, altering how the receptor can interact with other proteins within the cell. (wikipedia.org)
  • Two most abundant classes of transmembrane receptors are GPCR and single-pass transmembrane proteins. (wikipedia.org)
  • Intracellular signaling proteins: these pass the signal to the organelles of the cell. (wikipedia.org)
  • Target proteins: the conformations or other properties of the target proteins are altered when a signaling pathway is active and changes the behavior of the cell. (wikipedia.org)
  • Whole cell lysates were probed for NF-κB and IκB family proteins by immunoblot. (biomedcentral.com)
  • This cell-based gene-vector approach is ideal for delivering proteins to the CNS and has particular relevance to the control of inflammatory CNS disease. (ox.ac.uk)
  • The finding that pancreatic cancers also have KRAS mutations led the research group to explore whether T-cell receptor gene therapy that targets mutant KRAS proteins (neoantigens), could provide a positive therapeutic response for patients with pancreatic cancer as well. (cytologicsbio.com)
  • Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells. (nature.com)
  • Multiplexed quantification of proteins and transcripts in single cells. (nature.com)
  • These findings suggest that, in C6 glioma cells, c-fos mRNA induction induced by EAAs is not mediated by phosphorylation of ERK and CREB proteins. (kyobobook.co.kr)
  • Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. (duke.edu)
  • An increase in the threshold cycle (C T ) of a target sequence PCR product from activated cells was indicative of stimulation-induced chromatin remodelling. (biomedcentral.com)
  • Rao S, Gerondakis S, Woltring D, Shannon MF: c-Rel is required for chromatin remodeling across the IL-2 gene promoter. (biomedcentral.com)
  • Recent technological advances have enabled massively parallel chromatin profiling with scATAC-seq (single-cell assay for transposase accessible chromatin by sequencing). (nature.com)
  • Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
  • Additional strategies Chromatin immunoprecipitation accompanied by deep sequencing (ChIP-seq),1 viral planning,1 brief hairpin RNA (shRNA) testing,2 cloning of specific shRNAs and knockdown,6 quantitative polymerase string reaction,7 traditional western blotting,8 cell viability,9 and patient-derived xenograft versions10,11 had been performed mainly as previously referred to with additional information offered in supplemental Strategies (on the web page). (rawveronica.com)
  • risk of esophageal squamous cell carcinoma. (who.int)
  • PMID:18786442 mutations and the risk of esophageal squamous cell carcinoma. (who.int)
  • Arbyn M, Bergeron C, Klinkhamer P, Martin-Hirsch squamous cell carcinoma in a high-risk region in Iran. (who.int)
  • To identify genes, signaling pathways and networks that are controlled by SRC-3 and potentially important for hormone-dependent decidualization, we performed RNA-sequencing on HESCs in which SRC-3 levels were significantly reduced at the time of administering the deciduogenic stimulus. (frontiersin.org)
  • To characterize the biosafety profile of the CAR T cell products, we did the most comprehensive genomic insertion site analysis performed so far in T cells modified with SB. (uni-wuerzburg.de)
  • For T-cell receptor gamma targets, extracted genomic DNA from blood, lymph node, bone marrow, or other tissue types are PCR amplified and subjected to next generation sequencing (NGS) library preparation with a combination of heterogeneous TCR-gamma V(variable) region and J-(joining) region primers to generate TCR-gamma receptor VJ-rearranged templates, which are then subjected to next-generation sequencing on a MiSeq instrument. (ohsu.edu)
  • We determined the configuration of the genes for the beta (T beta) and gamma (T gamma) chains of the T cell receptor in DNA from 100 consecutive cases of B cell lymphoma and B cell chronic lymphocytic leukemia (B-CLL), and compared the findings with those in 18 T cell neoplasms. (jci.org)
  • Importantly, CD19-CAR T cells modified by MC-based SB transposition were equally effective as LV transduced CD19-CAR T cells in vitro and in a murine xenograft model (NSG/Raji-ffLuc), where a single administration of CD8+ and CD4+ CAR T cells led to complete eradication of lymphoma and memory formation of CAR T cells after lymphoma clearance. (uni-wuerzburg.de)
  • Lymphoma is a cancer that starts in cells that are part of the body's immune system. (invivoscribe.com)
  • PCR-based detection of rearranged T-cell receptor genes can be used to help establish a diagnosis of T-cell lymphoma, monitor for treatment response, and/or measure minimal residual disease (MRD). (ohsu.edu)
  • In the ovary, SR-BI was expressed in steroidogenically active theca cells in healthy follicles and in corpus luteum, where it may enhance cholesterol uptake as a substrate for steroid hormone production. (gu.se)
  • Sex steroid receptors (SSRs), which are involved in BC etiology and progression, promote BCSC proliferation, dedifferentiation and migration. (wjgnet.com)
  • ER) and progesterone receptor (PgR) parameters [1]. (lu.se)
  • Ingenuity Pathway Evaluation software program (Qiagen) was utilized to execute pathway and gene ontology evaluation of differentially controlled genes. (rawveronica.com)
  • Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy. (nih.gov)
  • The high level of gene transfer rate and efficient genome editing, superior safety profile as well as ease-of-handling and production of non-viral MC vectors and Cas9 RNP position our developed non-viral strategies to become preferred approaches in advanced cellular and gene-therapy. (uni-wuerzburg.de)
  • A single receptor may also be differently distributed at different membrane positions, depending on the sort of membrane and cellular function. (wikipedia.org)
  • We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
  • These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
  • Hepatitis C virus (HCV) infection has impaired cellular immune response and circulating NK cells, NK subsets (in- the ability to establish chronic infection virus persistence [8]. (who.int)
  • DNA microarray analyses using these cell lines were performed with or without adding each subtype ligand and provided much important information on the PPAR target genes involved in lipid metabolism, transport, storage and other activities. (biomedcentral.com)
  • Cells were grown in the presence or absence of 2.5 ng/ml murine TNF for 8 days. (biomedcentral.com)
  • Chronic culture of murine T cells in TNF does not alter the stability of IL-2 mRNA induced via TCR, or by PMA and ionomycin, in those cells. (biomedcentral.com)
  • In a murine relapsing experimental allergic encephalomyelitis (EAE) model, gene therapy to block TNF was investigated with the use of a retroviral dimeric p75 TNF receptor (dTNFR) construct. (ox.ac.uk)
  • Lymphocyte function should be assessed by measuring responses to phytohemagglutinin, a nonspecific stimulant of T-cell proliferation, concanavalin A directed at T-cell proliferation, and pokeweed mitogen directed at T-cell and B-cell proliferation. (medscape.com)
  • This process is vital to proliferation of T-cells in normal immune function, but can be exploited to aid in the distinction between reactive (benign) versus neoplastic processes of T-cell proliferation. (ohsu.edu)
  • A reactive, benign T-cell proliferation is characterized by polyclonal expansion of T-cells whereas a malignant process is characterized by clonal expansion of one T-cell population. (ohsu.edu)
  • Proliferation of tumor parenchyma cells was decreased. (nih.gov)
  • These molecular defects interfere with lymphocyte development and function, blocking the differentiation and proliferation of T cells and, in some types, of B cells and NK cells. (medscape.com)
  • In X-linked SCID, loss of IL-2 receptor (IL-2R) function leads to the loss of a lymphocyte proliferation signal. (medscape.com)
  • Consequently, there is a strong desire to make CAR T cell therapy scalable and globally available to patients. (uni-wuerzburg.de)
  • this response aids in defining the type of SCID but also is relevant to determining the need for immunosuppressive therapy in preparation for stem cell reconstitution. (medscape.com)
  • Gene therapy for chronic relapsing experimental allergic encephalomyelitis using cells expressing a novel soluble p75 dimeric TNF receptor. (ox.ac.uk)
  • Although the patient experienced short-lived toxic events associated with the preconditioning therapy that was provided days before the T-cell infusion, no adverse effects were seen with the engineered T cells. (cytologicsbio.com)
  • The research data obtained in these one-patient clinical trials warrant additional studies with more participants to determine the efficacy and safety of neoantigen T-cell receptor gene therapy for pancreatic and other mutant KRAS-expressing cancers. (cytologicsbio.com)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)
  • Using microarrays, a technology that allows thousands of genes to be examined in a single experiment, she hopes to identify genes that are regulated by the androgen receptor and other genes that progress to androgen-independence. (healthresearchbc.ca)
  • two with scant tumor cells one referral case with no tumor cells on the accompanying hematoxylin & eosin (H&E) slide and one with the assay being successfully conducted in the primary tumor. (ecologicalsgardens.com)
  • Locus controlling Bordetella pertussis-induced histamine sensitization (Bphs), an autoimmune disease-susceptibility gene, maps distal to T-cell receptor beta-chain gene on mouse chromosome 6. (ox.ac.uk)
  • In the mouse brain, we found that the plasticity inhibitors Nogo-A and Nogo receptor 1 (NgR1) are differentially expressed in the SVZ-OB system, in which Nogo-A identifies immature neuroblasts and NgR1 germinal astrocytes. (jneurosci.org)
  • The configuration of the TCR beta gene in primary tumor cells infected by HTLV-I was compared with that in the derived cell lines. (rupress.org)
  • In all pairs examined, the configuration in the primary tumor cells was different from that in the cell lines, strongly suggesting that the cells that grow in culture are not the original neoplastic cells. (rupress.org)
  • With the help of TCRs, the cells recognize foreign invaders and tumor cells. (ki.se)
  • Immunotherapy for patients with cancer involves the use of immune cells, often the patient's own cells, to develop a treatment to target tumor cells. (cytologicsbio.com)
  • Clark JM, Annenkov AE, Panesar M, Isomäki P, Chernajovsky Y, Cope AP: T cell receptor zeta reconstitution fails to restore responses of T cells rendered hyporesponsive by tumor necrosis factor alpha. (biomedcentral.com)
  • SCID is considered a pediatric emergency because survival depends on expeditious stem cell reconstitution, usually by bone marrow transplantation (BMT). (medscape.com)
  • T cell receptor gene studies allow B and T cell lymphomas to be distinguished from each other and from common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia. (jci.org)
  • Cell lines infected by HTLV-I express levels of TCR beta mRNA similar to PHA stimulated lymphocytes, suggesting that this gene is not transcriptionally activated as a result of infection by HTLV-I. Cloned T cells of known antigen specificity that are infected by HTLV-I in vitro show impairment of immune function, including loss of antigen-specific responsiveness and the acquisition of alloreactivity. (rupress.org)
  • T-cells that are part of our immune system are central in the protection against infections and cancer. (ki.se)
  • As these genes are among the most variable in our genome, the results also provide new information about how our immune system has developed over the course of history, says Martin Corcoran. (ki.se)
  • Normal plasma cells are found in the bone marrow and are an important part of the immune system. (invivoscribe.com)
  • HCC cell death, and natural killer (NK) cell activation and cytotoxicity were assessed in vitro after treatment with the TLR3 ligand poly(I:C). The effect of TLR3 on the tumor parenchyma and infiltrating immune cells was investigated in a spontaneous liver tumor mouse model and a transplanted tumor mouse model (n = 3-9 mice per group). (nih.gov)
  • In order to study the possible role over Ficoll-Hypaque (density 1.077 production, and are the key players in that NK cells might play in the patho- g/L) (Amersham Biosciences) and immune responses to viruses [7]. (who.int)
  • Molecular characterization of two novel alternative spliced variants of the KLRF1 gene and subcellular distribution of KLRF1 isoforms. (nih.gov)
  • The methods described in the study provide new opportunities, not the least in the cancer field where T-cells are central to several promising forms of immunotherapy", says Gunilla Karlsson Hedestam. (ki.se)
  • Peripheral blood mononuclear cells (PBMCs) were collected from the patient as the first step in creating the immunotherapy. (cytologicsbio.com)
  • We have previously described an in vitro model using human SCLC cell lines that express POMC and are resistant to glucocorticoids. (manchester.ac.uk)
  • The Association of Clinical Pathologists Molecular Pathology and Diagnostics Group in the United Kingdom also recommends that RAS analysis should include at least codons 12 13 59 61 117 and 146 of the KRAS gene and codons 12 13 59 and 61 of the gene.12 Massively parallel sequencing or next-generation sequencing technology has led to a revolution in genome discovery. (ecologicalsgardens.com)
  • It is the prototype of the primary immunodeficiency diseases and is caused by numerous molecular defects that lead to severe compromise in the number and function of T cells, B cells, and occasionally natural killer (NK) cells. (medscape.com)
  • In this study, we further characterize the properties of these T cells by analyzing their epitope specificity and T cell receptor (TCR) gene usage. (uni-luebeck.de)
  • abstract = "K- sam bek, N- sam flg and FGFR3 sam3 establish gene family of the receptors for heparin-binding growth factors (HBGFs) or FGFs. (elsevierpure.com)
  • this appears to have resulted in the presence of three alleles of the TCR beta gene, each of which is arranged differently. (rupress.org)
  • Men with more repeats within the regulatory region of the gene on both alleles had a significantly greater number of severely narrowed coronary arteries ( P = 0.009), larger areas of complicated lesions ( P = 0.008), and more calcifications of the coronary arteries ( P = 0.01) than men who had fewer repeats. (the-scientist.com)
  • In the process of signal transduction, ligand binding affects a cascading chemical change through the cell membrane. (wikipedia.org)
  • Dimerization: The dimerization model suggests that prior to ligand binding, receptors exist in a monomeric form. (wikipedia.org)
  • Rotation: Ligand binding to the extracellular part of the receptor induces a rotation (conformational change) of part of the receptor's transmembrane helices. (wikipedia.org)
  • For example, a neurotransmitter, hormone, or atomic ions may each bind to the extracellular domain as a ligand coupled to receptor. (wikipedia.org)
  • Klotho is an enzyme which effects a receptor to recognize the ligand (FGF23). (wikipedia.org)
  • In some receptors, such as members of the 7TM superfamily, the transmembrane domain includes a ligand binding pocket. (wikipedia.org)
  • Signal transduction processes through membrane receptors involve the external reactions, in which the ligand binds to a membrane receptor, and the internal reactions, in which intracellular response is triggered. (wikipedia.org)
  • human neuronal tissue.3C5 All three TRK receptors include an extracellular ligand-binding domain, a transmembrane region and an intracellular adenosine triphosphate-binding domain.2 6 TRK receptors are activated when neurotrophin ligands bind towards the extracellular site from the receptor (figure 1A). (researchtoactionforum.org)
  • DPhe 6 ,βAla 11 ,Phe 13 ,Nle 14 ]Bn(6−14) was found to have high affinity ( K i = 0.4 nM) for the fBB 4 receptor and 125 I-[DTyr 6 ,βala 11 ,Phe 13 ,Nle 14 ]Bn(6−14) to be an excellent ligand for this receptor. (acs.org)
  • Overall, our data suggest that polymorphisms in the host NKG2D and NKG2A genes have a protective role in SARS-CoV-2 infection, although the functional impact of these polymorphisms on control of SARS-CoV-2 infection remains unknown. (bvsalud.org)
  • gene polymorphisms and symptomatic differences in patients with narcolepsy type 1. (cdc.gov)
  • they react with the receptor to induce changes in the metabolism and activity of a cell. (wikipedia.org)
  • It was previously unknown how variable human TCR genes are", says Gunilla Karlsson Hedestam , professor at the department of microbiology, tumor and cell biology at Karolinska Institutet and the study's lead author. (ki.se)
  • Schier, A. F. Single-cell biology: beyond the sum of its parts. (nature.com)
  • Its pharmacology and cell biology are largely unknown, and no known natural cell lines or tissues possess sufficient numbers of fBB 4 -R's to allow either of these to be determined. (acs.org)
  • NKp80 is a marker of natural killer (NK) cell maturity in secondary lymphoid tissues and supports NK cell development through a stage 4a intermediate with ILC3-associated features. (nih.gov)
  • Draw lymphocyte markers at the same time as the CBC to obtain percentages and absolute counts of CD3 + T cells, CD4 + T cells, CD8 + T cells, CD19 + B cells, and natural killer (NK) cell markers (CD16 and CD56). (medscape.com)
  • The percentage of CD56(bright) cells was significantly higher than the control group ( P = 0.04). (who.int)
  • The frequency of CD3+CD56- T cells was significantly lower in both the chronic and resolved groups compared to the control group ( P = 0.04). (who.int)
  • The elimination or persistence (NKRs) and mediate functions of both cells and CD56+NT cells was charac- of the infection depends on a balance T cells and NK cells [13]. (who.int)
  • Chen L, Yu J (2016) Modulation of Toll-like receptor signaling in innate immunity by natural products. (malque.pub)
  • NK cells, a component of innate immunity, are variably affected. (medscape.com)
  • A complete absence of T-cell function by mitogen tests can occur in association with a normal lymphocyte count for age in some forms of SCID, including X-linked SCID (XL-SCID), in which all the lymphocytes are B cells. (medscape.com)
  • The B cell fragments were small in size and of limited size diversity when compared with the T cell cases, and germline bands of equal intensity were present. (jci.org)
  • There was a close correlation between agonist the receptor occupation and the receptor activation. (acs.org)
  • Some human small cell lung carcinomas (SCLC) secrete proopiomelanocortin (POMC) derived peptides, but in contrast to the pituitary, glucocorticoids fail to inhibit this hormone production. (manchester.ac.uk)
  • Cell surface receptors (membrane receptors, transmembrane receptors) are receptors that are embedded in the plasma membrane of cells. (wikipedia.org)
  • Transmembrane receptors in plasma membrane can usually be divided into three parts. (wikipedia.org)
  • We are studying the mechanisms by which chronic TNF suppresses T-cell responses in the CD4 + mouse T-cell hybridoma clone, 11A2. (biomedcentral.com)
  • Clinical and cell-based studies as well as investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological and pathophysiological contexts, underscoring their functional pleiotropy. (frontiersin.org)
  • Simultaneous epitope and transcriptome measurement in single cells. (nature.com)
  • While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
  • A high frequency of gene fusions have been identified in mammary analogue secretory carcinomas (90%C100%)11 and secretory breast carcinomas ( 90%)12 in adult patients, and in infantile fibrosarcomas (91%C100%),34 other mesenchymal tumours (100%)41 and congenital mesoblastic nephromas (83%)42 in paediatric patients. (researchtoactionforum.org)
  • In other receptors, the transmembrane domains undergo a conformational change upon binding, which affects intracellular conditions. (wikipedia.org)
  • The intracellular (or cytoplasmic) domain of the receptor interacts with the interior of the cell or organelle, relaying the signal. (wikipedia.org)
  • With enzyme-linked receptors, the intracellular domain has enzymatic activity. (wikipedia.org)
  • T-cell lymphomas account for approximately 15% of non-Hodgkin lymphomas and may pose a diagnostic challenge on the basis of histopathology alone and particularly in early stages of disease. (ohsu.edu)
  • The approach could also be adapted to engineer cells targeting other tumor specific neoantigens that may be more important in a given cancer. (cytologicsbio.com)