• Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. (hindawi.com)
  • Detection and quantification of cell viability and growth in two-dimensional (2D) and three-dimensional (3D) cell cultures commonly involve harvesting of cells and therefore requires a parallel set-up of several replicates for time-lapse or dose-response studies. (unboundmedicine.com)
  • Thus, developing a non-invasive and touch-free detection of cell growth in longitudinal studies of 3D tumor spheroid cultures or of stem cell regeneration remains a major unmet need. (unboundmedicine.com)
  • Taken together, these results demonstrate the use of SH-SAW device for detection and quantification of cell growth changes over time in 2D suspension cultures and in 3D cell culture models, which may have potential applications in both longitudinal 3D cell cultures in cancer biology and in regenerative medicine. (unboundmedicine.com)
  • This method enables detection and quantification of sub-nanomolar cytosolic siRNA release amounts from individual release events with measures of quantitation confidence for each event. (lu.se)
  • Procedures are provided for perturbation of cells using siRNA and reliable methodology for marker detection by indirect immunofluorescence in 96-well formats. (jove.com)
  • We describe here a new reversed-phase high-performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. (cas.cz)
  • Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
  • In this study, we present a novel nucleic acid-based method for the detection and possible quantification of CTCs utilizing quantitative TaqMan real time PCR. (iiarjournals.org)
  • However, they are present at nanomolar concentrations in biological samples, posing challenges for detection and quantification. (biorxiv.org)
  • This assay is based on a microbiological method conducted in petri dishes or multi-well cell culture plates. (wikipedia.org)
  • The focus forming assay (FFA) is a variation of the plaque assay, but instead of depending on cell lysis in order to detect plaque formation, the FFA employs immunostaining techniques using fluorescently labeled antibodies specific for a viral antigen to detect infected host cells and infectious virus particles before an actual plaque is formed. (wikipedia.org)
  • The FFA is particularly useful for quantifying classes of viruses that do not lyse the cell membranes, as these viruses would not be amenable to the plaque assay. (wikipedia.org)
  • Like the plaque assay, host cell monolayers are infected with various dilutions of the virus sample and allowed to incubate for a relatively brief incubation period (e.g., 24-72 hours) under a semisolid overlay medium that restricts the spread of infectious virus, creating localized clusters (foci) of infected cells. (wikipedia.org)
  • This endpoint dilution assay quantifies the amount of virus required to kill 50% of infected hosts or to produce a cytopathic effect in 50% of inoculated tissue culture cells. (wikipedia.org)
  • Here we report a direct fluorescence based DAPI assay system which removes the requirement for prior polyP extraction before quantification. (qub.ac.uk)
  • It provides a direct polyP assay which combines quantification capability with technical simplicity. (qub.ac.uk)
  • We describe herein a novel SARS-CoV-2 cell-cell transmission assay we developed which provides a very rapid and quantitative readout to assess SARS-CoV-2 Spike-hACE2 interaction in the absence of using pseudotyped particles or live virus. (mendeley.com)
  • The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. (ox.ac.uk)
  • Application of this new method will enable quantification of migration rates from in vitro scratch assay data that cannot be analysed using existing methods. (ox.ac.uk)
  • Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)
  • Presented is a flexible informatics workflow enabling multiplexed image-based analysis of fluorescently labeled cells. (jove.com)
  • The workflow described herein, incorporating STPT and automated quantification of fluorescently labeled features of interest, provides a framework by which one can objectively evaluate labeled neuronal or lymphocyte populations in healthy and injured brains. (frontiersin.org)
  • Guidelines for cell-type heterogeneity quantification based on a comparative analysis of reference-free DNA methylation deconvolution software. (ens-lyon.fr)
  • BACKGROUND: Cell-type heterogeneity of tumors is a key factor in tumor progression and response to chemotherapy. (ens-lyon.fr)
  • Tumor cell-type heterogeneity, definedas the proportion of the various cell-types in a tumor, can be inferred from DNAmethylation of surgical specimens. (ens-lyon.fr)
  • Gene expression heterogeneity is a key driver for microbial adaptation to fluctuating environmental conditions, cell differentiation and the evolution of species. (bath.ac.uk)
  • Melanoma tumour cells exhibit pronounced heterogeneity within a single tumour, amongst concurrent tumours within the same patient and amongst tumours from different patients. (nature.com)
  • Circulating tumour cells (CTCs), which are responsible for haematogenous spread of tumours, also reflect this heterogeneity. (nature.com)
  • Such heterogeneity could originate from extrinsic factors, such as cell-to-cell signalling and surrounding temperature and pressure, but also from the intrinsic noise generated by having few copies of molecules involved in transcription and translation. (lu.se)
  • Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. (iiarjournals.org)
  • Even then he presumed that these cells, today known as circulating tumour cells (CTCs), could play a role in the development of distant metastases. (iiarjournals.org)
  • Nowadays, a huge body of literature describes CTCs as cells that, after detaching from the primary tumour and circulating through the blood or lymphatic system, are the main cause of the occurrence of metastases in patients with epithelial cancer ( 2 - 4 ). (iiarjournals.org)
  • Currently, only one methodology for the discovery and enumeration of CTCs is approved, at least for the metastatic setting by the US Food and Drug Administration (FDA), namely the so-called Cell Search® System ( 10 ). (iiarjournals.org)
  • This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)
  • Circulating tumour cells (CTCs) can be assessed through a minimally invasive blood sample with potential utility as a predictive, prognostic and pharmacodynamic biomarker. (nature.com)
  • The heterogeneous expression of markers, together with their rare frequency (1-10 CTCs in 8 mL of blood) are the main intrinsic factors hampering CTC isolation and limiting in-depth study of these cells in patients with melanoma. (nature.com)
  • In this study, we compared the recovery and purity of melanoma cells isolated through different microfluidic devices, Parsortix (Angle plc, Surrey, United Kingdom) and ClearCell® FX1 system (Biolidics, Singapore), which enrich CTCs based on their differential cell size, density and deformability. (nature.com)
  • Patients with AR-V7-positive circulating tumor cells (CTCs) have greater benefit of taxane chemotherapy compared with novel hormonal therapies, indicating a treatment-selection biomarker. (eur.nl)
  • CONCLUSIONS: Padlock probe technology enables quantification of AR-V7, AR-FL, PSA, and KRAS mut/wt transcripts in CTCs. (eur.nl)
  • 100 predicted genomic off-target loci in 293T and 293FT cells. (cdc.gov)
  • Accurate quantification of cytosolic siRNA, and the establishment of the intracellular dose-response relationships, will aid the development and characterization of novel delivery strategies for nucleic acid therapeutics. (lu.se)
  • However, confounding factors known to associate with methylation values, such as age and sex, complicate accurate inference of cell-type proportions. (ens-lyon.fr)
  • A robust and accurate algorithm to acquire the 3D positions of the cells would help to reveal the mechanisms of embryogenesis. (nature.com)
  • This method achieves rapid and accurate quantification of total urinary arsenic. (cdc.gov)
  • We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
  • Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
  • Hans Anderson, Richard Weir, and John Caldwell of the University of Colorado have developed a completely automated method for the quantification of protein expression in immuno-labeled cells. (technologypublisher.com)
  • Stimulation of postsynaptic GABA B receptors generally triggers inhibition of adenylate cyclase and activation of G protein-gated inwardly rectifying K + (GIRK/Kir3) channels, leading to cell hyperpolarisation (Kaupmann et al. (springer.com)
  • To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
  • Bronchoalveolar lavage (BAL) was analysed for total and differential cell count, cell viability, and lipid and protein content. (cdc.gov)
  • No statistically significant differences in total cell counts, differential counts, viability, protein and lipid measurements, or lung histology were recognized among the 4 groups. (cdc.gov)
  • Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)
  • A lot happens in a cell when information is transferred from the genes' coding to a protein", explains Ákos Végvári, a researcher at the Division of Electrical Measurements at Lund University. (lu.se)
  • The same protein can exist in different forms and different concentrations in different cells. (lu.se)
  • Single-cell kinetics of siRNA-mediated knockdown in cells expressing destabilized eGFP unveiled a dose-response relationship with respect to knockdown induction, depth and duration in the range from several hundred to thousands of cytosolic siRNA molecules. (lu.se)
  • Stained endocrine cell nuclei are segmented by a sequential thresholding algorithm that uses a Support Vector Machine (SVM) type of artificial neural network [ 9 ]. (hindawi.com)
  • Detailed quantification of post-stroke neural connectivity and neuroinflammation indicates a role for remote brain regions in stroke pathology and recovery. (frontiersin.org)
  • The results provide region-specific quantification of neural connectivity and neuroinflammation, which could be a critical tool for investigating mechanisms of not only stroke recovery, but also a wide variety of brain injuries or diseases. (frontiersin.org)
  • To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)
  • Blood vessels (BVs) are considered an integral component of neural stem cells (NSCs) niches. (elifesciences.org)
  • Neural stem cells (NSCs) serve as a source for new neurons in both the developing and adult brain. (elifesciences.org)
  • Here we quantify radiation-induced changes in cell migration as part of the physics of cancer: a novel research frontier unraveling the roles of physical properties of cells in cancer. (aps.org)
  • The segmentation and quantification of cell nuclei are two very significant tasks in the analysis of histological images. (hindawi.com)
  • In this paper, an automated entropy-based thresholding system for segmentation and quantification of cell nuclei from histologically stained images has been presented. (hindawi.com)
  • Adapting to robust automated image processing techniques for primary tasks such as cell nuclei segmentation and quantification will not only prove to be time efficient for pathologists, but these techniques will also be capable of producing consistent results. (hindawi.com)
  • In recent years, numerous image processing techniques have been proposed for cell nuclei segmentation [ 2 ]. (hindawi.com)
  • While some techniques only perform the task of cell nuclei segmentation and quantification, techniques that are capable of further detecting and classifying abnormal tumors (cell nuclei) that cause various types of cancer have also been proposed. (hindawi.com)
  • A cell nuclei segmentation algorithm incorporating unsupervised color clustering, morphological operations, and local thresholding has been proposed to distinguish the cancerous and noncancerous areas in histologically stained images and then segment the clustered cell nuclei [ 3 ]. (hindawi.com)
  • K-means clustering is implemented as unsupervised color clustering technique for cell nuclei segmentation in [ 4 ]. (hindawi.com)
  • Entropic-based thresholding methods for cell nuclei segmentation are proposed by Wang and Gudla et al. (hindawi.com)
  • An adaptive-attention-window-(AAW-) based cell nuclei segmentation technique that exploits quadtree decomposition is proposed by Ko et al. (hindawi.com)
  • and 6) cell density-distribution quantification ( Figure F ). Segmentation performance was assessed by comparing with manual cell counts. (atlantis-press.com)
  • When applied to in vitro data, our method outperforms standard methods by detecting differences in the migration rates between different cell groups that the other methods could not detect. (ox.ac.uk)
  • HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
  • Yeast extract, or other varieties of yeast hydrolysate, have been used in combination with a lipid supplement to completely eliminate the need for fetal bovine serum during in vitro cultivations of insect cells. (uwaterloo.ca)
  • Such a method of live-cell lipid quantification has (i) experimental power in basic cell biology, (ii) practical utility for identifying developmental predictive biomarkers while advancing biology-based oocyte/embryo selection, and (iii) ability to yield rationally supporting technology for decision-making in rodents, domestic species, and human assisted reproduction and/or fertility preservation. (rsc.org)
  • The plasma membranes of lens fibre cells are one of the most cholesterol rich membranes in the human body, forming lipid rafts and contributing to the biophysical properties of lens fibre plasma membrane. (aston.ac.uk)
  • In situ hybridization and immunohistochemical studies have shown that Purkinje cells (PCs), the output neurons of the cerebellar cortex, are the neuron type with the highest levels of GABA B receptors (Bowery et al. (springer.com)
  • Research into this novel virus will be facilitated by the availability of clearly described and effective protocols that enable the propagation and quantification of infectious virus. (cdc.gov)
  • In addition, results showed that SAW measurements per se did not affect viability of cells. (unboundmedicine.com)
  • Tomographic images were analyzed to count the AC cells, and to calculate to absolute measurements of aqueous signal intensity. (unimi.it)
  • Conclusions: Anterior segment SS-OCT could be used for a comprehensive assessment of AC inflammation, providing objective measurements of inflammatory cells and aqueous flare. (unimi.it)
  • T-cell numbers were derived from three measurements deter- attributable to the decrease in the number of T cells that bear mined with two different instruments, a hematology analyzer the CD4 receptor ( 5-9 ). (cdc.gov)
  • BZK induced a significant increase in the severity of the clinical score, infiltration of inflammatory cells, local expression of inflammatory cytokines in subcutaneous tissue, and total serum immunoglobulin (Ig) E. PVP-I increased the clinical score, number of mast cells, and production of inflammatory cytokines, and total serum IgE. (medsci.org)
  • Et-OH increased the clinical score and number of inflammatory cells, but showed no effect on serum IgE levels. (medsci.org)
  • Methods: Participants underwent an AC inflammation evaluation including: clinical cell and flare grading and laser flare photometry (LFP). (unimi.it)
  • Main Outcome Measures: Correlation between OCT-derived AC inflammatory indexes (ARI index and AC cells on OCT) and standard clinical techniques (LFP, clinical cell grading). (unimi.it)
  • Purpose: To assess the ability of swept-source (SS) optical coherence tomography (OCT) of the anterior segment (AS) to measure anterior chamber (AC) inflammation (both flare and cells) objectively. (unimi.it)
  • Single-cell kinetics of siRNA-mediated knockdown in cells expressing destabilized eGFP unveiled a. (lu.se)
  • The primary tasks involved in the analysis of histologically stained tissue sections are cell nuclei counting, detecting abnormal cell nuclei, and the presence of antigens within the target cells. (hindawi.com)
  • For example, the production of virus-based vaccines, recombinant proteins using viral vectors, and viral antigens all require virus quantification to continually monitor and/or modify the process in order to optimize product quality and production yields and to respond to ever changing demands and applications. (wikipedia.org)
  • A 3D COMSOL model was built to simulate the mass loading response of increasing concentrations of cells in suspension in the polydimethylsiloxane (PDMS) well in order to predict the characteristics and optimize the design of the SH-SAW biosensor. (unboundmedicine.com)
  • These controls are prepared as whole cell or nucleic acid mixtures comprising three genetically engineered bacterial strains (derived from Escherichia coli , Staphylococcus aureus , and Clostridium perfringens ), each containing a unique synthetic DNA tag that can be detected and quantified in routine 16S rRNA gene amplicon and shotgun sequencing assays. (atcc.org)
  • The simulated relative frequency shift from the two oscillatory circuit systems (one of which functions as control) were found to be concordant to experimental data generated with RAW264.7 macrophage and A549 cancer cells. (unboundmedicine.com)
  • Current quantification methods of scratch assays deal poorly with irregular cell-free areas and crooked leading edges which are features typically present in the experimental data. (ox.ac.uk)
  • We introduce a new migration quantification method, called 'monolayer edge velocimetry', that permits analysis of low-quality experimental data and better statistical classification of migration rates than standard quantification methods. (ox.ac.uk)
  • Transcriptome sequencing-based classification and quantification of IKZF1 transcript isoforms in B-cell acute lymphoblastic leukemia]. (bvsalud.org)
  • To classify and quantify IKZF1 mutant transcripts in B- cell acute lymphoblastic leukemia (B-ALL) by RNA sequencing ( RNA-seq ) and bioinformatics analysis . (bvsalud.org)
  • Despite significant advances in understanding nephron segment patterning, many questions remain about the underlying genes and signaling pathways that orchestrate renal progenitor cell fate choices and regulate differentiation. (mdpi.com)
  • A switch from glycolysis to mitochondrial respiration drives these cells toward differentiation, but the mechanisms that control this switch are poorly defined. (lww.com)
  • M, Peterson C. 2018 Haematopoietic stem suggests that during differentiation a stem cell approaches a stable equili- cells: entropic landscapes of differentiation. (lu.se)
  • Single cells in these populations exhibit different com- stem cell differentiation, Shannon information binations of regulator activity that suggest the presence of multiple theory, entropy configurations of a potential differentiation network as a result of multiple entry points into the committed state. (lu.se)
  • However, as the result of recent advancements in microscopic imaging technology and computational image processing techniques [ 1 ], there has been significant growth of research towards translational computational systems that can detect, analyze, classify, and quantify cell nuclei from microscopic images. (hindawi.com)
  • Naive e450 + -labeled splenic CD8 + cytotoxic T cells were intravenously injected at 7, 24, 48, and 72 h post-tMCAo. (frontiersin.org)
  • Nephron progenitors, the cell population that give rise to the functional unit of the kidney, are metabolically active and self-renew under glycolytic conditions. (lww.com)
  • Recent preclinical radio-manganese PET studies have shown promise for the non-invasive quantification of functional pancreatic β-cell mass (fBCM). (aapm.org)
  • This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)
  • We propose a method for automatic SMC quantification, and assessed its potential using a murine SMC apoptosis model. (atlantis-press.com)
  • Histogram of `A MAE` (`A MAE`: Mean Absolute Error on estimated A, the matrix of cell proportions) for 10 D matrices (mean value of 10 random noises applied on D) computed from 10 random A. Each color represents a different method. (biomedcentral.com)
  • The new method relies on quantifying the horizontal component of the cell monolayer velocity across the leading edge. (ox.ac.uk)
  • The method described in this manual assesses arsenic exposure by analyzing urine through the use of inductively coupled-plasma dynamic reaction cell-mass spectrometry (ICP-DRC-MS). Urine is analyzed because urinary excretion is the major pathway for eliminating arsenic from the mammalian body (Vahter ME, 1988). (cdc.gov)
  • The influx of macrophages and accumulation of T-cells and myofibroblast were quantified in orbital connective tissue. (frontiersin.org)
  • Yet despite the significant advances made in polyP research there is a lack of rapid and efficient analytical approaches for the quantification of polyP accumulation in microbial cultures and environmental samples. (qub.ac.uk)
  • While reference-free algorithms have been developed to infer cell-type proportions from DNA methylation, a comparative evaluation of the performance of these methods is still lacking. (ens-lyon.fr)
  • D) Coinciding nuclei, corresponding with the left orange nuclei in C, shown in grey, with cell cores shown in red and blue (step 4, Methods). (atlantis-press.com)
  • Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. (iiarjournals.org)
  • While advanced cell-labeling methods have accelerated the field of neuroscience, specifically in brain mapping, there remains a need to quantify and analyze the data. (eneuro.org)
  • Other examples of specific instances where viruses need to be quantified include clone screening, multiplicity of infection (MOI) optimization, and adaptation of methods to cell culture. (wikipedia.org)
  • There are many ways to categorize virus quantification methods. (wikipedia.org)
  • Although bench methods like flow cytometry and RNA-sequencing can characterize immune responses with single-cell resolution, whole murine colon specimens are processed at once. (biorxiv.org)
  • Robust and standardised methods for the quantification of cannabinoids within botanical and drug forms is a critical step forward for an emerging Cannabis-based pharmaceutical industry, which is poised for rapid expansion. (researchgate.net)
  • Despite a growing body of analytical methods for the quantification of cannabinoids, few have been validated using internationally accredited guidelines. (researchgate.net)
  • Validation parameters for establishing robust standardised methods for cannabinoid quantification within Cannabis-based drug forms are critically discussed. (researchgate.net)
  • The "In vivo Brain Circuit Analysis" platform is a research infrastructure devoted to integrating methods and techniques to interrogate neuronal circuits in health and disease using advanced methods for the perturbation and recording of cell activity. (lu.se)
  • Lungs were examined microscopically for quantification of severity of injury. (cdc.gov)
  • 30 nm in diameter, establish direct membrane-to-membrane contact with endothelial cells in specialized areas of irregular endothelial basement membrane and enriched with vesicular activity. (elifesciences.org)
  • Cell migration is a crucial step in cancer metastasis, the complex process which accounts for over 90% of cancer-related deaths. (aps.org)
  • There is emerging evidence that radiotherapeutic doses meant to kill cancer cells can promote metastasis by enhancing cell migration. (aps.org)
  • To assess cell migration post-irradiation, we used a commercially available device, ECIS Z-Theta, which non-invasively measures and converts complex cell-substrate electric impedance into series resistance and capacitance in real time. (aps.org)
  • 0.01) enhanced migration compared to non-irradiated cells, within the first 40 hours following irradiation with 20 Gy. (aps.org)
  • Our results suggest cell migration as a new therapeutic target in anti-metastasis strategies for improved radiotherapy outcomes. (aps.org)
  • On the other hand, summarizing current findings also shows that both single cells and entire cell sheets, tissues or organisms respond to curvature by modulating their shape and their migration behavior. (ox.ac.uk)
  • Surface curvature both emerges from, and influences the behavior of, living objects at length scales ranging from cell membranes to single cells to tissues and organs. (ox.ac.uk)
  • Macrophage influx was positively linked to the extent of T cell influx and fibroblast activation within GO-affected orbital tissues. (frontiersin.org)
  • Its metastatic potential depends on the phenotype of the tumor cells, and it frequently disseminates before diagnosis. (medscape.com)
  • Tumor-induced glaucoma may be produced by obstruction of outflow pathways by pigment cells (pigment dispersion syndrome), melanin-laden macrophages (melanomalytic glaucoma), or tumor cells. (medscape.com)
  • Since surface acoustic waves (SAWs) permit mass loading-based biosensing and have been touted due to their many advantages including low cost, small size and ease of assembly, we examined the potential of SAW-biosensing to detect and quantify cell growth. (unboundmedicine.com)
  • CONCLUSION: Based on the lessons learned, to facilitate pipeline validation and catalyze further pipeline improvement by the community, we develop a benchmark pipeline for inference of cell-type proportions and implement it in the R package medepir. (ens-lyon.fr)
  • An integrated bioinformatics pipeline was developed to adapt the classification and quantification of IKZF1 transcripts from RNA-seq and was applied to sequencing data of these cases. (bvsalud.org)
  • We have established two well-behaved stable cell lines which have demonstrated a remarkable correlation with standard cell-free viral pseudotyping for inhibition by convalescent sera, post-vaccine sera, small molecule drugs, and murine anti-spike monoclonal antibodies. (mendeley.com)
  • It can be effectively used or modified for high throughput screening for compounds and biologics that interfere with virus-cell binding and entry to complement other neutralization assays currently in use. (mendeley.com)
  • In many diseases, retrospective analysis for determining the presence of mast cells has been difficult because of their loss of metachromatic staining properties once tissue has undergone formalin fixation. (nih.gov)
  • We quantified mast cells in peribronchiolar tissue of idiopathic pulmonary fibrosis (IPF) and in normal human lung by using rabbit antiserum to human mast cell tryptase. (nih.gov)
  • In addition, mast cells in cases of IPF had an altered appearance--irregularity of the plasma membrane and release of extracellular tryptase. (nih.gov)
  • We conclude that the number of mast cells is increased in IPF and that the altered appearance of the mast cells suggests that they are activated and undergoing degranulation. (nih.gov)
  • The increased intestinal permeability was accompanied by augmented expression of genes marking immune cell infiltration and encoding key proteins for immune cell adhesion, antigen presentation, and cytokine signaling in the orbital tissue. (frontiersin.org)
  • We used a standard laboratory irradiator to irradiate both non-cancer (HCN2 neurons) and cancer cells (T98G glioblastoma) with 2 Gy, 10 Gy and 20 Gy of X-rays. (aps.org)
  • 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunode- ficiency virus [HIV]. (cdc.gov)
  • In this regard, we used blood samples from a healthy donor mixed with different numbers of established breast cancer cells (CAMA-1, MCF7, ZR-75-1) and carried out subsequent gene expression analysis for cytokeratins 8, 18 and 19. (iiarjournals.org)
  • Slice Histology Alignment, Registration, and Probe-Track analysis), we introduce a post-imaging analysis tool to map histological images to established mouse brain atlases called SHARCQ (Slice Histology Alignment, Registration, and Cell Quantification). (eneuro.org)
  • In summary, SHARCQ was designed as a neuroscience post-imaging analysis tool for cell-to-brain registration and quantification with a simple, accessible interface. (eneuro.org)
  • Advancements in genetically distinct cell-labeling have begun to outpace the analysis required to quantify it. (eneuro.org)
  • A major drawback is the need to extract polyP from cells prior to analysis. (qub.ac.uk)
  • Here, we show that quantification of 'Involved' and 'Uninvolved' image patch classes in swiss rolls of colonic specimens can be utilized to train a linear determinant analysis classifier to distinguish between mouse models. (biorxiv.org)
  • This averaging effect hampered the analysis of finer regulatory mechanisms at the single-cell level, the fundamental unit for any fate decision process. (lu.se)
  • Specifically, a confluent monolayer of host cells is infected by applying a sample containing the virus at varying dilutions and then covered with a semi-solid medium, such as agar or carboxymethyl cellulose, to prevent the virus infection from spreading indiscriminately, as would occur in a liquid medium. (wikipedia.org)
  • A viral plaque is formed after a virus infects a cell within the fixed cell monolayer. (wikipedia.org)
  • By embryonic day 15.5, kidneys of nephron progenitor cell-specific VHL knockout mice begin to exhibit reduced maturation of nephron progenitors. (lww.com)
  • RUNX1-positive leukemia cells in preclinical models. (lu.se)
  • Tools for refined cell-specific targeting have significantly contributed to understanding the characteristics and dynamics of distinct cellular populations by brain region. (eneuro.org)
  • SHARCQ produces quantitative and qualitative data, including counts of brain-wide region populations and a 3D model of registered cells within the atlas space. (eneuro.org)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • Further, SH-SAW biosensing was applied to A549 cells cultured on a 3D electrospun nanofiber scaffold that generate tumor spheroids (tumoroids) and the results showed the device's ability to detect changes in tumor spheroid growth over the course of eight days. (unboundmedicine.com)
  • Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. (plos.org)
  • Here, we describe protocols for the propagation of SARS-CoV-2 in Vero E6 cells as well as two human cells lines, the intestinal epithelial Caco-2 cell line and the respiratory epithelial Calu-3 cell line. (cdc.gov)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • The melanoma cell lines A2058 and SK-MEL-5 were used in spiking experiments due to their differential cell size. (nature.com)
  • Because the cells in embryos are considerably crowded, an algorithm to segment individual cells in detail and accurately is needed. (nature.com)
  • In early embryos, cells are loosely connected to each other. (nature.com)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
  • Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
  • The program requires MATLAB, histological images, and either a manual or automatic cell count of the unprocessed images. (eneuro.org)
  • As early as 1869, cells with the morphology of cancer cells had been found by Thomas Ashworth in the blood of a person who died from metastatic cancer ( 1 ). (iiarjournals.org)