• Cellular senescence-inhibited gene (CSIG), also named as ribosomal_L1 domain-containing 1 (RSL1D1), is implicated in various processes including cell cycle regulation, cellular senescence, apoptosis, and tumor metastasis. (transhumanist.ru)
  • ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
  • We also found that P. paniceum treatment increased apoptosis and arrested of the cell cycle at G0/G1 remarkably when compared with the control group. (wjon.org)
  • P. paniceum did not possess any toxicity (cytotoxic and mutagenic) but has the potential for anticancer activity against human colorectal cells by increasing apoptosis, which leads to the suppression of cell proliferation. (wjon.org)
  • Gain of function and loss of function experiments showed that PDCD4 induced turnover (proliferation and apoptosis) of HUVECs. (plos.org)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. (plos.org)
  • SPIN1 ablation activates p53, suppresses cell growth, reduces clonogenic ability, and induces apoptosis of human cancer cells. (elifesciences.org)
  • Histone deacetylases (HDACs) play critical roles in apoptosis and contribute to the proliferation of cancer cells. (oncotarget.com)
  • Treatment with AR-42 decreased HCC cell growth and increased caspase-dependent apoptosis, and this was rescued by HDAC5 overexpression. (oncotarget.com)
  • Taken together, these results demonstrate for the first time that AR-42 targets HDAC5 and induces apoptosis in human hepatocellular carcinoma cells. (oncotarget.com)
  • However, in the presence of Aza despite increased ERV-K113 env expression, an inhibition of HEK293 proliferation and a further restriction of invasion was found. (frontiersin.org)
  • TP53 inhibition allows LINE-1 + cells to grow, and genome-wide-knockout screens show that these cells require replication-coupled DNA-repair pathways, replication-stress signaling and replication-fork restart factors. (nih.gov)
  • Using our cell-penetrating peptides (CPPs) from the IDDT platform, we identified RMNE1 and its derivative RMNE3, "DualPep-Shine", which showed levels of α-Melanocyte stimulating hormone (α-MSH)-induced melanin inhibition comparable to the conventional tyrosinase inhibitor, Kojic acid. (bvsalud.org)
  • Here we show that both the pan-BET inhibitor (+)-JQ1 as well as a newly developed specific isoxazole inhibitor, PNZ5, showed potent inhibition of gastric cancer cell growth. (ox.ac.uk)
  • Intriguingly, we found differences in the antiproliferative response between gastric cancer cells tested derived from Brazilian patients as compared to those from Asian patients, the latter being largely resistant to BET inhibition. (ox.ac.uk)
  • By contrast, the combination of UHRF1 depletion and histone deacetylase (HDAC) inhibition reactivated the silenced genes and strongly suppressed CRC cell proliferation. (biomedcentral.com)
  • The combination of UHRF1 depletion and HDAC inhibition also induced marked changes in the gene expression profiles such that cell cycle-related genes were strikingly downregulated. (biomedcentral.com)
  • Surprisingly, neither pharmacological inhibition of VRAC by carbenoxolone, 4-[(2-Butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]butanoic acid (DCPIB) or 5-nitro-2-(3-phenylpropyl-amino)benzoic acid (NPPB), nor siRNA-mediated knockdown or disruption of the essential VRAC subunit LRRC8A affected cell proliferation and migration in any of the investigated cell lines. (fu-berlin.de)
  • I also found that inhibition of VRAC with DCPIB or siRNA against LRRC8A has no effect on PI3K/Akt signaling in glioblastoma cells. (fu-berlin.de)
  • For many years it was assumed that HEK 293 cells were generated by transformation of either a fibroblastic, endothelial or epithelial cell, all of which are abundant in kidneys. (wikipedia.org)
  • TNFSF18 is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. (genscript.com)
  • TNFSF18 / GITR Ligand is important for interactions between activated T-lymphocytes and endothelial cells.Recombinant Human GITR Ligand Fc Chimera produced in HEK293 cells is a polypeptide chain containing 359 amino acids with the C-termimal human IgG1 Fc fragment. (genscript.com)
  • Interleukin-1β (IL-1β) is a non-secreted pro-inflammatory cytokine produced primarily by activated macrophages as well as neutrophils, epithelial cells and endothelial cells. (abmole.com)
  • GM-CSF is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. (abmole.com)
  • Recombinant Human VEGF165 Protein (HEK293, C-His) is a powerful growth and angiogenic cytokine, it promotes the proliferation and survival of endothelial cells, promotes angiogenesis and vascular permeability. (abmole.com)
  • Attenuate hepatic lipogenesis and gluconeogenesis, regulate NO production in endothelial cells, inhibit proliferation of smooth muscle cells and prevent lipid accumulation of macrophage cells. (prospecbio.com)
  • We investigated whether atheroprotective unidirectional pulsatile shear stress affects the expression of PDCD4 in endothelial cells. (plos.org)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
  • However, the action of PDCD4 in endothelial cells is unclear. (plos.org)
  • Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • Choroidal endothelial cells (RF/6A) were maintained in a semiconfluent state and hypoxia was induced by exposing the cells to cobalt chloride for 24 h. (molvis.org)
  • However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
  • c KLHL18 protein co-immunoprecipitated with CUL3 protein in HEK-293 T cells. (biomedcentral.com)
  • In this research, we studied the ability of TC on antioxidative activity and the efficacy of TC on interleukin-17 (IL-17A)-induced intracellular oxidative stress, inflammation-relative, and proliferation-relative protein expression in human keratinocytes. (bvsalud.org)
  • Interferon α 2A (IFN-α 2A) belongs to the type I interferon protein family and is produced by many cell types in response to viral infection. (sigmaaldrich.com)
  • Recombinant Human PDGF-AA Protein (HEK293) is a potent mitogen for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. (abmole.com)
  • Recombinant Human IL-11 Protein (HEK293) is a multifunctional cytokine produced by stromal cells such as fibroblasts, epithelial cells and osteoclasts. (abmole.com)
  • Western blot analysis of whole cell lysates probed with Mouse anti Human proliferation associated protein 2G4 antibody ( VMA00111 ) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P ) and visualized on the ChemiDoc MP with 28 second exposure. (bio-rad-antibodies.com)
  • Arrow points to proliferation associated protein 2G4 (molecular weight 44 kDa). (bio-rad-antibodies.com)
  • The TSC2 gene encodes for the protein Tuberin, a tumour suppressor protein well known for it's ability to regulated cell growth and the cell cycle. (uwindsor.ca)
  • Having a better understanding of how specific changes in this large protein alters fundamental cell biology such as cell proliferation and cell size can ultimately help to effectively treat patients with these specific mutations. (uwindsor.ca)
  • binding research, purified GST fusion protein immobilized over the Sepharose beads had been incubated with His-tagged PIPKIi2 purified from bacterias or with cell lysates ready from HEK293 cells transfected with HA-PIPKIi2 at 4 C for 1 h Vandetanib accompanied by elution of destined protein with 2 test Vandetanib buffer for immunoblotting. (healthweeks.com)
  • In a human prostate carcinoma cell line, small interfering RNA (siRNA)-mediated knockdown of USP9X reduced IGF-IR as well as IRS-2 protein levels and increased their ubiquitination. (oncotarget.com)
  • The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. (cancerindex.org)
  • The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)
  • Recombinant Human TGF-β1 (HEK293) regulates cell proliferation, growth, differentiation and cells movement. (abmole.com)
  • Our results show that the Tuberin/CycB1 interaction regulates cell size and this regulation is nutrient dependent. (uwindsor.ca)
  • Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
  • MZ1257RC showed induction of ERV-K113 expression and significantly increased both proliferation and invasion in the presence or absence of Aza. (frontiersin.org)
  • HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
  • In addition, ERV-K env significantly regulates proliferation and invasion depending on p53 status and Aza treatment. (frontiersin.org)
  • DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
  • Downregulation of Herg1 suppresses osteosarcoma proliferation and invasion by targeting Hippo signaling pathway]. (cancerindex.org)
  • P. paniceum also inhibits cell migration and exerts antimutagenicity, thereby suggesting that P. paniceum might be useful for colorectal cancer treatment. (wjon.org)
  • Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
  • We therefore undertook a preliminary screening study of the three IFBTS seed oils in vitro using human colon adenocarcinoma cells (Caco-2), a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. (iiarjournals.org)
  • Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
  • 2016 ). This study was conducted to analyze the anticancer effects of green-synthesized AgNPs using Juniperus polycarpos on A549 (adenocarcinomic human alveolar basal epithelial cells) cell lines, as well as comparing it with Cisplatin commercial drug. (springeropen.com)
  • It presents a functional unit of a physical barrier consisting of a mucus layer and a monolayer of epithelial cells and of a mucosal lymphoid system that together efficiently discriminate between pathogenic and commensal microorganisms 16 . (nature.com)
  • De novo formation of an adherens junction (AJ) between single epithelial cells requires coordinated, spatial actin dynamics, but the mechanisms steering nascent actin polymerization for cell-cell adhesion initiation are not well understood. (rupress.org)
  • Here we investigated real-time actin assembly during daughter cell-cell adhesion formation in human breast epithelial cells in 3D environments. (rupress.org)
  • We identify formin-like 2 (FMNL2) as being specifically required for actin assembly and turnover at newly formed cell-cell contacts as well as for human epithelial lumen formation. (rupress.org)
  • Optogenetic control of Rac1 in living cells rapidly drove FMNL2 to epithelial cell-cell contact zones. (rupress.org)
  • Human colon adenocarcinoma (Caco-2) and human embryonic kidney (HEK-293) cells were maintained and treated with various concentrations (0, 20, 40, 80, 100 and 120 mg/l) of K. africana, M. zehyeri and X. caffra seed oil. (iiarjournals.org)
  • In the present study human embryonic kidney (HEK 293) cells were used to study the growth after repeated exposure to SNP. (scirp.org)
  • Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973. (wikipedia.org)
  • HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
  • Given the location of the adrenal gland (adrenal means "next to the kidney"), a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. (wikipedia.org)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • HEK293 (Human embryonic kidney cell line). (prospecbio.com)
  • In addition, the HEK293 human embryonic kidney stem cell line was also used. (healthykidneyclub.com)
  • For evaluating the phosphatidylinositol 4,5-biphosphate distribution in the PIPKIi2 or PIPKI knockdown cells, MDA-MB-231 cells had been transfected with siRNA as defined above. (healthweeks.com)
  • IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells. (abmole.com)
  • We show that Arg2 is the dominant enzyme in dendritic cells (DCs) and is repressed by microRNA-155 (miR155) during their maturation. (unige.ch)
  • The last mentioned has become specifically essential in the framework of illnesses like tumor where common 4-Aminohippuric Acid treatments (e.g., rays and chemotherapy) derive from the substantial induction of tumor cell loss of life. (treatmentforprostatecancer.info)
  • Contemporary anti-cancer therapies purpose at inducing immunogenic tumor cell loss of life. (treatmentforprostatecancer.info)
  • Animal-derived oils and purified fatty acids, but not indigenous fruit-tree-derived seed oils, have been used to study cell growth and differentiation. (iiarjournals.org)
  • The trypan blue dye exclusion method was used to determine cell growth 48-hours after oil treatment. (iiarjournals.org)
  • All three tree seed oils suppressed both Caco-2 and HEK-293 cell growth in a dose-dependent manner. (iiarjournals.org)
  • K. africana oil significantly suppressed Caco-2 cell growth compared to HEK-293 cell growth at all oil concentrations, whereas M. zeyheri and X. caffra seed oils significantly suppressed HEK-293 and Caco-2 cell growth, only at a concentration of 80 mg/l. (iiarjournals.org)
  • HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. (wikipedia.org)
  • We identified a colorectal cancer wherein a fast-growing tumor subclone downregulated LINE-1, prompting us to examine how LINE-1 expression affects cell growth. (nih.gov)
  • We find that nontransformed cells undergo a TP53-dependent growth arrest and activate interferon signaling in response to LINE-1. (nih.gov)
  • Glycosylation contributes to stability in cell growth media and other applications. (sigmaaldrich.com)
  • Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family. (abmole.com)
  • The tunability of the hydrogel gives the ability to create an optimized environment for cell growth. (thewellbio.com)
  • Here, I systematically examined the role of VRAC during cell growth and motility for various cell types, including C2C12 myoblasts, HCT116 cells, HEK-293 cells and U251 and U87 glioblastoma cells. (fu-berlin.de)
  • Insulin-like growth factors (IGFs) have been shown to induce proliferation of many types of cells. (oncotarget.com)
  • Knockdown of USP9X significantly reduced anchorage-independent cell growth of prostate carcinoma cell line. (oncotarget.com)
  • Our study describes RNF8 as a co-activator of ERalpha increases ERalpha stability via post-transcriptional pathway, and provides a new insight into mechanisms for RNF8 to promote cell growth of ERalpha-positive breast cancer. (polyplus-sartorius.com)
  • This study shows that expression profiling can be used to identify genes that are transcriptionally or post-transcriptionally modified following CSIG knockdown and to reveal the molecular mechanism of cell proliferation and senescence regulated by CSIG. (transhumanist.ru)
  • Stably introduced into cancer cell lines, the system reports on elevated endogenous ADAR1 editing activity induced by interferon as well as knockdown of ADAR1 and ADAR2. (cancerindex.org)
  • Although PDCD4 in general suppresses the development and progression of tumors, its specific biological functions differ by cell type [8] . (plos.org)
  • Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)
  • Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
  • Hypoxia of CEC was induced by exposing the cells to cobalt chloride (Sigma-Aldrich, St. Louis MO) in serum media, as described previously [ 12 , 13 ], and the induction was confirmed by cytotoxicity and cell cycle analysis. (molvis.org)
  • In all experiments, cells were seeded 24 h before induction. (healthykidneyclub.com)
  • Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
  • Furthermore, we investigated the correlated expression patterns of Cdc14B, ESCO1 , KPNA5 , MAP3K3 , and CSIG during cell cycle and senescence progression, which imply the important pathways CSIG regulating cell cycle and senescence. (transhumanist.ru)
  • The aim of our study was to examine the suppressive proliferation, anti-migration and mutagenic/antimutagenic properties of P. paniceum . (wjon.org)
  • Moreover, P. paniceum could decrease the migration of HCT116 and HT29 cancer cells. (wjon.org)
  • Although VRAC's potential physiological functions and activation mechanism were studied extensively, conflicting results were obtained regarding the role of VRAC in cell proliferation and migration, as well as its activation mechanism. (fu-berlin.de)
  • These results indicate that, contrasting the prevailing assumption in the literature, VRAC is dispensable for cell proliferation and migration. (fu-berlin.de)
  • DMH-1 blocks BMP4-induced phosphorylation of Smads 1, 5 and 8 in HEK293 cells. (tocris.com)
  • Src phosphorylation of tyrosine residues on the C terminus of PIPKIi2 (Tyr residues in Fig. 1indicate Src phosphorylation … To research the role of every PIPKI splice variant, PIPKI variations had been portrayed into MDA-MB-231 cells ectopically, which express a minimal degree of PIPKI/PIPKIi2 weighed against other breast cancers cell lines analyzed (not proven). (healthweeks.com)
  • IFN-α 2A enhances the expression of MHC proteins by increasing the presentation of antigen peptides and activates cytotoxic T cells. (sigmaaldrich.com)
  • these T cell responses targeted multiple proteins and were capable of recognizing heterologous strains (genotypes 1A and 3A). (ox.ac.uk)
  • Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. (medchemexpress.com)
  • creation price = 1.37 10?2 RLU/min/cell) and DUOX2/DUOXA2 overexpression steady HEK293 cells (baseline H2O2 production price = 1.0 10?4 RFU/min/cell), respectively, treated with 521 for 30 min. (likemap.me)
  • The viability of the HEK-293 normal cells was found to be significantly higher than the viability of the cancerous cells at all of the tested concentrations, thus suggesting that all the compounds have better inhibitory activity on the cancer cell line. (mendeley.com)
  • To investigate P. paniceum 's anticancer ability, HCT116 and HT29 cell lines were treated with a water extract containing P. paniceum , and then the cell viability was examined using the trypan blue exclusion method which were compared to HEK293 (non-cancerous cells). (wjon.org)
  • Treatment with P. paniceum caused a loss of cell viability in HCT116 and HT29 cells (not found in HEK293), which had an IC 50 (half-maximal inhibitory concentration) of 1,156.2 and 1,207.0 µg/mL, respectively. (wjon.org)
  • TNFSF18 Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. (genscript.com)
  • Cancer cells are characterized by dual DNA methylation-related changes: global hypomethylation, which can induce chromosomal instability and oncogene activation, and regional hypermethylation, which is associated with transcriptional silencing. (biomedcentral.com)
  • These results indicate that miR155-induced repression of Arg2 expression is critical for the ability of DCs to drive T cell activation by controlling arginine availability in the extracellular environment. (unige.ch)
  • Novel adenovirus-based vaccines induce broad and sustained T cell responses to HCV in man. (ox.ac.uk)
  • These data indicate that an adenoviral vector strategy can induce sustained T cell responses of a magnitude and quality associated with protective immunity and open the way for studies of prophylactic and therapeutic vaccines for HCV. (ox.ac.uk)
  • miR155 is known to be strongly induced in various mouse and human DC subsets in response to diverse maturation signals, and miR155-deficient DCs exhibit an impaired ability to induce Ag-specific T cell responses. (unige.ch)
  • Because the suggestions for drug screening process in antineoplastic remedies need evaluation of individual tumors xenotransplanted into immune-compromised 4-Aminohippuric Acid mice (5), the function from the immune system continues to be neglected (6), producing studies centered on the interplay between disease fighting capability and dying cells required. (treatmentforprostatecancer.info)
  • This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments. (cancerindex.org)
  • Cell adhesion molecule-1 (CADM1) is a member of the immunoglobulin superfamily that functions as a tumor suppressor of lung tumors. (cancerindex.org)
  • Repair of mitomycin-C-induced chromosomal aberrations was impaired in RAD51B/Rad51b-c.92delT human and mouse somatic cells in vitro and in explanted mouse bone marrow cells. (nature.com)
  • From 3D cell culture, 2D cell coating to animal injection, VitroGel makes it possible to bridge the in vitro and in viv o studies with the same platform system. (thewellbio.com)
  • Finally, in vitro and in vivo functional assays using DCs exhibiting deregulated Arg2 expression indicated that Arg2-mediated arginine depletion in the extracellular milieu impairs T cell proliferation. (unige.ch)
  • The suppression of Caco-2 and HEK-293 cell proliferation by K. africana, M. zeyheri and X. caffra seed oils suggest a potential antiproliferative effect of these tree seed oils on the two cell lines. (iiarjournals.org)
  • Cyclic GMP was able to mimic the antiproliferative effect of SNP on HEK293 cells. (scirp.org)
  • The antiproliferative effect of cGMP increased from 30% to 89% and the cGMP in increased from 240 to 480 pmol/10 7 cells. (scirp.org)
  • This study shows that the antiproliferative effects of SNP on HEK293 cells are mediated through cGMP - dependent and cGMP-independent mecha nisms. (scirp.org)
  • The anticancer activity of these compounds was evaluated in colon cancer, HCT-116, and noncancerous, HEK-293, cell lines using the MTT antiproliferative assays. (mendeley.com)
  • All the compounds exhibited antiproliferative activity against the HCT-116 cell line with IC50 values at 33 ± 1.25, 36 ± 2.25, 34 ± 2.15, 32 ± 2.35, 34 ± 2.65, and 36 ± 1.95 μg/mL for compounds 1 to 6, respectively. (mendeley.com)
  • c-FLIP increased Zellmotilit ht t r A further the importance of c-FLIP is its involvement in motility of cancer cells obtained ht. (syksignaling.com)
  • Erythropoietin (EPO) is a glycoprotein hormone known primarily for its role in erythropoiesis, which is responsible for stimulating the proliferation and differentiation of erythroid progenitor cells. (abmole.com)
  • DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
  • Using site-directed mutagenesis we constructed six TSC2 mutants to study the phenotypes in HEK293 and NIH3T3 cells. (uwindsor.ca)
  • Transfecting these cell lines with a codon optimized ERV-K113 env overexpressing CMV vector was performed with or without 5'-Aza-2'-deoxycytidine (Aza) treatment to sustain promoter de-methylation. (frontiersin.org)
  • In mammals, functional analysis of the individual RAD51 paralogues in cell lines has shown similar but non-redundant contributions in DNA repair processes such as HR efficiency, RAD51 nuclear focus formation, sensitization to mitomycin C (MMC) and protection of perturbed replications forks [ 11 ]. (nature.com)
  • d , e KLHL18 inhibited the expression of PI3Kp85α via the Rbx1-CUL3-KLHL18 complex in d NCI-A549 and (E) NCI-H1299 cell lines. (biomedcentral.com)
  • The expression of caspase-3 and caspase-9 genes increased in HCT-116 cell lines after 48 h of treatment, suggesting cell death by the apoptotic pathways. (mendeley.com)
  • In order to explore new treatment options in this target area we have screened a library of epigenetic inhibitors against gastric cancer cell lines and identified inhibitors for the BET family of bromodomains as potent inhibitors of gastric cancer cell proliferations. (ox.ac.uk)
  • 2018 ). It was also confirmed that AgNPs synthesized by the Juniperus chinensis leaf showed the potent anticancer effect on the human adenocarcinoma gastric (AGS) cell lines (Al-Dhafri and Ching 2019 ). (springeropen.com)
  • CRC cell lines were transiently transfected with siRNAs targeting UHRF1 , after which DNA methylation was analyzed using dot blots, bisulfite pyrosequencing, and Infinium HumanMethylation450 BeadChip assays. (biomedcentral.com)
  • A team of researchers from Weill Cornell Medicine and Mount Sinai Hospital developed two novel models of humanized mice using five human embryonic stem cell lines. (healthykidneyclub.com)
  • Four human embyonic stem cell lines were used in the experiment. (healthykidneyclub.com)
  • As the transgenes are integrated at the same chromosomal site, cell lines were prepared by pooling individual colonies. (healthykidneyclub.com)
  • Cell lines with less than 5% blue cells were used for further experiments. (healthykidneyclub.com)
  • There are multiple aborted fetal cell lines currently in use, and each one can be traced back to a preborn baby. (healthykidneyclub.com)
  • Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
  • Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
  • The cell lines in our cell bank have been successfully used in gene-editing cell line generation, applicable for all kinds of gene-editing experiments. (ubigene.us)
  • AR-42 is a novel Class I and II HDAC inhibitor that shows cytotoxicity against various human cancer cell lines. (oncotarget.com)
  • Depletion of UHRF1 rapidly induced genome-wide DNA demethylation in CRC cells. (biomedcentral.com)
  • Importantly, we are inserting the Tuberin-C698Y mutation into the HEK293 cells genome through the CRISPR-Cas9 system to determine the endogenous significance of this specific change. (uwindsor.ca)
  • Garg, U.C. and Hassid, A. (1989) Nitric Oxide-Generating Vasodilators and 8-Bromo-cyclic Guanosine Monophosphate Inhibit Mitogenesis and Proliferation of Cultured Rat Vascular Smooth Muscle Cells. (scirp.org)
  • ED50= 3-8.5 μg/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF. (prospecbio.com)
  • The acute and chronic effects of sodium nitroprusside (SNP) are well characterized for vascular smooth muscle cells (VSMC). (scirp.org)
  • However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
  • The HEK-293 cell line has been used extensively ( 16 - 17 ) as a laboratory work-horse in several cell culture studies. (iiarjournals.org)
  • 293T (or HEK 293T) is a derivative human cell line that expresses a mutant version of the SV40 large T antigen. (wikipedia.org)
  • After presumably adapting to tissue culture, cells from this clone developed into the relatively stable HEK 293 line. (wikipedia.org)
  • An embryonic adrenal precursor cell therefore seems the most likely origin cell of the HEK 293 line. (wikipedia.org)
  • The first reference to the cell line as "293T" may be its use to create the BOSC23 packaging cell line for producing retroviral particles. (wikipedia.org)
  • All the compounds produced a significant reduction in HCT-116 cell line proliferation, except compounds 2 and 6. (mendeley.com)
  • Overall, the results revealed that the green-synthetized AgNPs had anti-metastasis and anti-proliferation effects on lung cancer cells in comparison to cisplatin with lower side effects on the normal cell line. (springeropen.com)
  • The cell line was developed by J.P. Jacobs in September 1966 after an abortion was committed on a physically healthy 27-year-old woman for psychiatric reasons. (healthykidneyclub.com)
  • To find the mechanism that exerts a suppressive proliferation effect on P. paniceum , we determined the DNA fragmentation and cell cycle distribution. (wjon.org)
  • We found that P. paniceum significantly inhibited the proliferative function of HCT116 and HT29 cells. (wjon.org)
  • When cGMP (1000 μM) was added to the cell culture medium for 5 days the cell densities were reduced with 37% below baseline and cGMP in increased from 5.3 to 195 pmol/10 7 cells. (scirp.org)
  • HumanKine IFN-α 2A, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 16 kDa. (sigmaaldrich.com)
  • Included in these are intrinsic cell immunogenicity, the type of the original loss of life stimulus, the sort of harm linked molecular patterns (DAMPs) released, the clearance capability from the affected tissues for useless and dying cells, as well as the particular loss of life pathway. (treatmentforprostatecancer.info)
  • Further, this study elucidated the cellular and molecular mechanisms of nanoparticles for anti-proliferative and apoptotic effects on human lung cancer cells (A549) and compared them with commercial drug cisplatin. (springeropen.com)
  • AgNPs are utilized for treating different types of cancerous cells due to their extraordinary biomedical features (anti-cancer, anti-bacterial, anti-viral, and anti-angiogenic properties) as well as surface-specific characteristics (Burdușel et al. (springeropen.com)
  • Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries. (cancerindex.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Here, we have explored RNF8 as an associated partner of ERalpha in breast cancer cells, and co-activates ERalpha-mediated transactivation. (polyplus-sartorius.com)
  • Good for adhesion cells or cells requiring stronger cell-matrix interactions. (thewellbio.com)
  • Open up in another screen Fig. 6 Substance 521The inhibitory ramifications of 521 on HT-29 cell development, whole-cell ROS creation, mobile respiration, and extracellular ROS creation were evaluated using the same strategies defined above for the various other DPI analogs. (likemap.me)
  • The compound can also be used in protocols for the chemical reprogramming of somatic cells to iPSCs. (tocris.com)
  • BALB/c mice immunized with allogeneic dying melanoma cells expressing revCasp-3 or CpnTCTD demonstrated strong rejection from the allogeneic problem. (treatmentforprostatecancer.info)
  • On the other hand, mice immunized with cells dying either after appearance of tBid or irradiation with UVB didn't, recommending an silent cell death immunologically. (treatmentforprostatecancer.info)
  • In this scholarly study, we demonstrated how different kinds and systems of cell loss of life, induced by different stimuli, influence the results of allogeneic tumor transplants in BALB/c immune-competent mice. (treatmentforprostatecancer.info)
  • Antigen standard for annexin A4 (ANXA4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Many studies suggest that host T cell responses are critical for spontaneous resolution of disease, and preclinical studies have indicated a requirement for T cells in protection against challenge. (ox.ac.uk)