• They include endothelial cells, fibroblasts, and keratinocytes. (frontiersin.org)
  • Conditioned medium from adipose derived stem cells (ADSC-CM) stimulates both collagen synthesis and migration of fibroblasts, and accelerates wound healing in vivo. (indexindex.com)
  • After ADSC-CM addition, collagen type I, type III, HAS and hyaluronic acid (HA) expressions on human dermal fibroblasts (HDFs) were evaluated. (indexindex.com)
  • FBN2 silencing recapitulates hypoxic conditions and induces elastic fiber impairment in human dermal fibroblasts. (mcgill.ca)
  • Using Foxn1-/- and Foxn1+/+ mice, we demonstrated that epidermally expressed Foxn1 regulates dWAT development and defines the adipogenic capacity of dermal fibroblasts. (bvsalud.org)
  • Furthermore, Foxn1 activity strengthens adipogenic processes through Bmp2 and Igf2 signaling and regulates lipid metabolism in differentiated dermal fibroblasts. (bvsalud.org)
  • Aggressive, transformed or transfected mesenchymal cells containing proto-oncogenes can act in the absence of lymphocytes, but whether these cells represent regressed fibroblasts, chondrocytes or bone marrow stem cells is unclear. (biomedcentral.com)
  • Subsequently, the scaffold was bioconjugated with apelin-13 using the EDC-NHS linker and acellular scaffolds were recellularized using fibroblasts, endothelial cells, and smooth muscle cells. (biomedcentral.com)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • Applying MPTR to dermal fibroblasts from middle-aged donors, we found that cells temporarily lose and then reacquire their fibroblast identity, possibly as a result of epigenetic memory at enhancers and/or persistent expression of some fibroblast genes. (elifesciences.org)
  • In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
  • Generally, wound healing in the oral cavity is known to occur more quickly and scar less than dermal tissue, which may be due to the elements in saliva and unique phenotype of oral fibroblasts ( 14 - 16 ). (spandidos-publications.com)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. (molcells.org)
  • Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. (molcells.org)
  • Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing. (molcells.org)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • Furthermore, oral fibroblasts generally required more attempts for successful iPS generation as opposed to dermal fibroblasts. (biomedcentral.com)
  • The oral fibroblasts generally proved to be more difficult to reprogram than dermal fibroblasts. (biomedcentral.com)
  • Xeno-free dermal, buccal and gingival fibroblasts can successfully generate iPS with a comparable genotype/phenotype to their xenogenic counterparts. (biomedcentral.com)
  • Fibroblasts are key mediators in wound healing and tissues repair. (biomedcentral.com)
  • In this study, we used PDGFR-specific neutralising antibodies to dissect out receptor-specific signalling events in fibroblasts in vitro , to further understand key cellular processes involved in wound healing and tissue repair. (biomedcentral.com)
  • Neutralising antibodies against PDGFRs were shown to block signalling through PDGFRα and PDGFRβ receptors, reduce human PDGF-AA and PDGF-BB-induced collagen gel remodelling in dermal fibroblasts, and reduce migration stimulated by all PDGF ligands in human dermal and lung fibroblasts. (biomedcentral.com)
  • Platelet-derived growth factors (PDGFs) acting via their tyrosine kinase receptors are major mitogens for many cell types of mesenchymal origin, including fibroblasts and vascular smooth muscle cells (VSMCs)[ 1 - 4 ]. (biomedcentral.com)
  • The prenatal population of dermal macrophages is gradually replaced by the recruitment and differentiation of circulating progenitors in adulthood. (wikipedia.org)
  • In this process, cytokines like CSF1 are essential in facilitating the differentiation of monocytes into tissue-resident dermal macrophages and their survival. (wikipedia.org)
  • Therefore, the differentiation of monocytes to dermal macrophages is CSF-1/CSF1R dependent. (wikipedia.org)
  • Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
  • MSCs were characterized for plastic adhesion, surface markers, proliferation and differentiation capacity. (biomedcentral.com)
  • These multipotent cells with innate self-renewal capacity can be in vitro expanded without losing their differentiation potential. (biomedcentral.com)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • Although most cells have vitamin D receptors, several key genes that encode proteins and peptides are modulated in part by vitamin D and thus affect musculoskeletal functions, mitochondrial respiration, cell growth, proliferation, differentiation, and apoptosis [13]. (vitamindwiki.com)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
  • Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
  • The in vitro effects of MSC-Exo on immune cell migration and responder T cell proliferation were examined by chemotactic assays and lymphocyte proliferation assays, respectively. (nature.com)
  • These results suggest that MSC-Exo effectively ameliorate EAU by inhibiting the migration of inflammatory cells, indicating a potential novel therapy of MSC-Exo for uveitis. (nature.com)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • Integrin-based effects on growth factor re-ceptors include enhancement of cell migration (11, 12), survival (13), and proliferation (14 -17). (123dok.org)
  • Cell viability, adhesion and migration of primary cultured RP cells were observed in the presence of Tβ4 at various concentrations ranging from 1 to 1,000 ng/ml. (spandidos-publications.com)
  • Tβ4 did not affect cell viability and adhesion, but RP cell migration was stimulated by Tβ4 at concentrations of 100 and 1,000 ng/ml. (spandidos-publications.com)
  • Enhancement of RP cell migration and angiogenesis is likely to be involved in the promotion of wound healing. (spandidos-publications.com)
  • Upregulation of Tβ 4 by cDNA-mediated transfection was found to cause actin depolymerization in fibroblast cells ( 1 ), which demonstrated that the modulation of Tβ 4 affected the polymerization state of actin cytoskeleton, as well as other cell processes associated with the organization of actin cytoskeleton, such as cell migration. (spandidos-publications.com)
  • Ito et al ( 2 ) showed that overexpression of Tβ 4 enhanced the formation of actin-based pseudopodia and cell motility in prostate cancer cells, providing direct evidence that Tβ 4 plays a role in cell migration. (spandidos-publications.com)
  • Colon cancer cells overexpressing Tβ 4 also exhibited enhanced cell migration, due in part to the activation of the Rac1 signaling pathway ( 3 ). (spandidos-publications.com)
  • In addition, extracellular, administered Tβ 4 promoted the cell migration of various types of cells including cardiomyocytes, human umbilical vein endothelial cells and conjunctival epithelial cells ( 4 - 6 ). (spandidos-publications.com)
  • The mechanism by which exogenous Tβ 4 influences cell migration remains to be elucidated. (spandidos-publications.com)
  • Besides the effect on cell migration, exogenous Tβ 4 was found to have multifunctional activities such as angiogenesis, anti-apoptosis, anti-oxidative stress and anti-inflammation ( 8 - 11 ), which emphasize its therapeutic potential in the repair of damaged tissues or wound healing. (spandidos-publications.com)
  • Hence, it is of great importance to promote the proliferation and migration of denatured dermis. (molcells.org)
  • The timeline for cell migration in a normal wound healing process is predictable. (medscape.com)
  • In vitro , PDGFR isoforms have been shown to be potent activators of fibroblast proliferation, migration and survival[ 10 ]. (biomedcentral.com)
  • We further discuss how injuries alter tissue environments and how this primes mononuclear phagocytes to enforce this particular environment, for example, to support host defense and pathogen clearance, to support the resolution of inflammation, to support epithelial and mesenchymal healing, and to support the resolution of fibrosis to the smallest possible scar. (hindawi.com)
  • These complex events involve three overlapping phases: inflammation, proliferation and remodelling [ 1 - 4 ]. (biomedcentral.com)
  • Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
  • After a short period, capillary vasodilatation occurs secondary to local histamine release, and the cells of inflammation are able to migrate to the wound bed. (medscape.com)
  • The fetal defects observed in congenital rubella syndrome are likely secondary to vasculitis resulting in tissue necrosis without inflammation. (medscape.com)
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • The mRNA and protein expression of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factor (VEGF) in Tβ4-treated RP cells was assessed by quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. (spandidos-publications.com)
  • 19 - 21 Other signals, such as those from endothelial cells (ECs) located at the DP, are also essential for HF maintenance. (benitonovas.com)
  • the endothelial cell is also an organizer and a regulator of healing. (docksci.com)
  • It is also thought to be involved in VSMC phenotypic modulation and is upregulated in endothelial cells exposed to atherosclerosis-prone flow patterns[ 18 ]. (biomedcentral.com)
  • Superficial lesions consist of dilated lymph vessels lined by flat endothelial cells in a discontinuous layer immediately subjacent to the oral epithelium. (medscape.com)
  • Negative WT1 staining implies a nonproliferative nature of the oral lymphangiomas, thus growth is most likely secondary to enlargement of lymphatic vessels and not neoplastic proliferation of lymphatic endothelial cells. (medscape.com)
  • The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
  • On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)
  • The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)
  • Extracellular matrix scaffolds derived from porcine small intestinal submucosa (SIS-ECM) have been shown to promote the formation of site-specific tissue in a number of preclinical animal studies. (researchgate.net)
  • However, efficient harvest and handling of cell sheets remain challenging, including insufficient extracellular matrix content and poor mechanical strength. (researchgate.net)
  • Mechanical loading has been widely used to enhance extracellular matrix production in a variety of cell types. (researchgate.net)
  • We found that the extracellular matrix content and thickness of cell sheet were markedly elevated upon appropriate mechanical conditioning. (researchgate.net)
  • The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
  • The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)
  • We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
  • The present study performed on abdominal human intact and scar skin focused on determining gender differences in extracellular matrix (ECM) composition, dermal white adipose tissue (dWAT) accumulation, and Foxn1 expression as a part of the skin response to injury. (bvsalud.org)
  • Our results proposed 16-h perfusion of 0.25% sodium dodecyl sulfate (SDS) + 0.5% Triton X-100 combination to the vessel as an optimal decellularization protocol in terms of cell elimination as well as extracellular matrix preservation. (biomedcentral.com)
  • 8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
  • Their role in enhancing migratory and proliferative responses and extracellular matrix (ECM) synthesis in these cells makes them key regulators of critical biological and pathological functions including tissue remodelling, scarring and fibrosis. (biomedcentral.com)
  • Details on the available acute oral and dermal toxicity studies are provided in Tables 7 and 8 . (canada.ca)
  • 2006). New studies have attempted cancer risk assessments based on exposure assessments via oral and dermal/inhalation routes (Liao et al. (who.int)
  • Our podiatrists approach these chronic wounds with a combination of two therapies: a bilayered living cellular construct (BLCC) and an acellular fetal bovine collagen dressing (FBCD). (footankleinstitute.com)
  • Therefore, we aimed to compare the efficiency of using humanized culture conditions, specifically human platelet lysate to fetal bovine serum, for iPS generation from different sources, and to evaluate their stemness. (biomedcentral.com)
  • Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
  • they may designate phases as the hemostasis phase, inflammatory phase, proliferation phase, and remodeling phase, or they may refer to the hemostasis phase, inflammatory phase, granulation phase, and maturation phase. (medscape.com)
  • [ 4 ] Therefore, certain phases have more than one name, such as remodeling or maturation and proliferation or granulation. (medscape.com)
  • By the fourth day, phagocytosis of contaminated tissues is well underway, and the processes of epithelization, collagen deposition, and maturation are occurring. (medscape.com)
  • Upon maturation, the cell sheets were harvested by lowering the temperature. (researchgate.net)
  • Recent work has demonstrated that the epigenome is already rejuvenated by the maturation phase of somatic cell reprogramming, which suggests full reprogramming is not required to reverse ageing of somatic cells. (elifesciences.org)
  • This study describes a novel "maturation phase transient reprogramming" (MPTR) method to restore the epigenome of cells to a more youthful state. (elifesciences.org)
  • Dermal macrophages are macrophages in the skin that facilitate skin homeostasis by mediating wound repair, hair growth, and salt balance. (wikipedia.org)
  • Wound healing is a complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. (medscape.com)
  • This process can go awry and produce an exuberance of fibroblastic proliferation with a resultant hypertrophic scar , which by definition is confined to the wound site. (medscape.com)
  • Although various categories of wound healing have been described, the ultimate outcome of any healing process is repair of a tissue defect. (medscape.com)
  • The subsequent wound healing processes aim to restore dermal and epidermal integrity and functionality. (biomedcentral.com)
  • In this context, regulatory T cells (Tregs) hold a key role in balancing immune homeostasis and mediating cutaneous wound healing. (biomedcentral.com)
  • The maintenance of tissue homeostasis and proper wound healing is, therefore, a critical process that can be disturbed by a plethora of external and internal factors. (biomedcentral.com)
  • Specifically, the timely orchestration of pro- and anti-inflammatory responses, based on the activation and inhibition of various cell types, such as tissue-resident cells and different types of immune cells, can significantly impact wound repair [ 7 ]. (biomedcentral.com)
  • While researchers agree on the abundance of Tregs in distinct dermal layers, the exact involvement of Tregs in cutaneous wound healing remains to be elucidated [ 18 ]. (biomedcentral.com)
  • Mononuclear phagocytes contributions to tissue pathologies relate to their central roles in orchestrating all stages of host defense and wound healing, which often become maladaptive processes, especially in sterile and/or diffuse tissue injuries. (hindawi.com)
  • Advances in tissue engineering technology have led to the production of novel human skin equivalents and organoids that reproduce cell-cell interactions with tissue-scale tensional homeostasis, and enable us to evaluate skin tissue morphology, functionality, drug response and wound healing. (mdpi.com)
  • These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. (rbcp.org.br)
  • The aim of this pre-clinical study was to investigate the effects of intradermal injections of MSC in experimental cutaneous wound repair comparing allogeneic and autologous adipose stem cells (ASCs) and autologous bone marrow-mesenchymal stromal cells (BM-MSCs). (biomedcentral.com)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • The use of collagen dressings in conjunction with other treatment modalities like negative pressure wound therapy (NPWT) can help facilitate adherence of the tissue layers in the wound bed and improve healing. (footankleinstitute.com)
  • Exogenous Tβ 4 was also reported to accelerate the tissue repair of damaged cardiac, corneal and dermal tissues ( 4 , 6 , 13 ), which demonstrates potential for clinical applications in wound healing. (spandidos-publications.com)
  • Despite the relatively rapid wound healing, however, tissues damaged during periodontal and implant surgery are continuously challenged by bacterial infection in the oral cavity, necessitating meticulous maintenance of oral hygiene and additional plaque control. (spandidos-publications.com)
  • Department of Cell and Structural Biology, University of Manchester M I3 9PL, U.K. t Department of ,tmmunology, University of Liverpool, P.O. Box 147, L69 3BX, U.K. Abstraet--Angiogenesis is an essential component of wound healing. (docksci.com)
  • Wound healing cannot occur without angiogenesis, The vasculature comprises up to 60% of repair tissue (Dyson et aI. (docksci.com)
  • Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). (molcells.org)
  • Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
  • Understanding how the body repairs damaged tissue and what factors influence the wound healing process helps the surgeon ensure an acceptable outcome from surgery. (medscape.com)
  • Wound healing in any tissue follows a predictable sequence of events. (medscape.com)
  • Although seemingly basic in concept, advances in molecular science have allowed modern medicine to gain a true appreciation of the complex interplay between the cells involved in the phases of wound healing. (medscape.com)
  • As greater understanding of the growth factors involved in wound healing emerges, future patient care may include scarless wound healing and transplant of tissues engineered from stem cell progenitors. (medscape.com)
  • After injury to tissue occurs, the cell membranes, damaged from the wound formation, release thromboxane A2 and prostaglandin 2-alpha, potent vasoconstrictors. (medscape.com)
  • Cells involved in wound healing. (medscape.com)
  • The cells appearing in a wound are depicted in sequence from left to right, and the color bars represent the range of days each cell type is in the wound. (medscape.com)
  • 48,49 In addition, cyclic mechanical strain enhances the function and development of engineered tissues by improving the production of collagen and elastin. (researchgate.net)
  • Scar skin of men showed highly increased levels of COLLAGEN 1A1, COLLAGEN 6A3, and ELASTIN mRNA expression, the accumulation of thick collagen I-positive fibers, and the accumulation of α-SMA-positive cells in comparison to the scar skin of women. (bvsalud.org)
  • Other profibrotic, collagen synthesis stimulating cytokines include endothelin, interleukin (IL)-1 and mast cell tryptase. (biomedcentral.com)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • Y-27632 eye drops (or vehicle only) were administered to eyes after a superficial keratectomy, and the tissue was examined by immunohistochemistry for α-SMA, collagen types I, II, and III, and keratan sulfate. (molvis.org)
  • Interestingly, bundles of aligned and uniformly spaced collagen fibrils were more prevalent in keratocytes in Y-27632-treated corneas, which is reminiscent of fibripositor-like structures that have been proposed as a mechanism of matrix deposition in embryonic connective tissues. (molvis.org)
  • The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
  • Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
  • Mesenchymal stromal cells (MSCs) expanded in vitro have been proposed as a potential therapy for congenital or acquired skin defects in pediatrics. (biomedcentral.com)
  • Mesenchymal stromal cells were in vitro expanded from adipose and BM tissues of young female New Zealand rabbits. (biomedcentral.com)
  • In this review, we discuss the potential influence of stromal vascular fraction from adipose tissue in the management of alopecia as well as its involvement in preclinical and clinical trials. (jcadonline.com)
  • however, a variety of other cell types are found as well, collectively labeled as stromal vascular fraction (SVF). (jcadonline.com)
  • Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
  • Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)
  • Current research have demonstrated that maternal anti-CD36 antibodies signify a frequent explanation for fetal/neonatal alloimmune thrombocytopenia (FNAIT) in Asian and African populations. (aabioetica.org)
  • Intradermal adipocytes form dermal white adipose tissue (dWAT), a unique fat depot localized in the lower layer of the dermis. (bvsalud.org)
  • Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)
  • 26,27 Dermal adipocytes are organized in small clusters under the reticular dermis and interact with the hair follicular cells regulating the hair cycle. (jcadonline.com)
  • Dermal macrophages' ability to acquire pro-inflammatory properties also potentiates them in cancer defence. (wikipedia.org)
  • Dermal macrophages are native immune cells mainly located in the dermis of the skin. (wikipedia.org)
  • Dermal macrophages belong to the mononuclear phagocyte system that serves a vital role in the innate immunity of the skin. (wikipedia.org)
  • They are also a type of antigen-presenting cells (APCs) that can mediate the infiltration of immune cells during an immune response, suggesting dermal macrophages' influence on both the innate and adaptive immune systems. (wikipedia.org)
  • Dermal macrophages are either from embryonic progenitors or circulating progenitors. (wikipedia.org)
  • Numerous dermal macrophages are present in the skin at birth due to the infiltration of yolk-sac derived macrophages and haematopoietic stem cell (HSC)-derived monocytes. (wikipedia.org)
  • Prenatal dermal macrophages are not to be confused with Langerhans cells as they uniquely express CD14 and FXIIIa and lack CD1a. (wikipedia.org)
  • The prenatal portion of tissue-resident dermal macrophages is produced from yolk-sac derived precursors. (wikipedia.org)
  • The generation of dermal macrophages results from primitive haematopoiesis or definitive haematopoiesis. (wikipedia.org)
  • Primitive haematopoiesis allows the generation of yolk-sac derived macrophages and subsequent release into the foetal bloodstream for tissue infiltration and colonisation. (wikipedia.org)
  • The level of dermal macrophages from prenatal development remains detectible through constant, slow proliferation. (wikipedia.org)
  • The postnatal population of dermal macrophages is achieved by the infiltration of circulating monocytes given proper CCR2 signalling, a pathway responding to chemokines. (wikipedia.org)
  • The infiltration of bone-marrow-derived monocytes generated postnatally creates a distinct population of dermal macrophages. (wikipedia.org)
  • The generation of dermal macrophages is highlighted by the expression of cell markers such as CD64, MERTK and the downregulation of CCR2. (wikipedia.org)
  • Dermal macrophages can phagocytose and digest foreign substances similar to other cell types in the mononuclear phagocyte system. (wikipedia.org)
  • Dermal macrophages have a distinct expression of genes to facilitate their specialisation in removing macromolecules and foreign pathogens. (wikipedia.org)
  • Dermal macrophages' functions suggest their importance in the skin's innate immunity. (wikipedia.org)
  • Dermal macrophages, Langerhans cells and dendritic cells are the main types of antigen-presenting cells (APCs) in the skin. (wikipedia.org)
  • Foreign materials are walled off by macrophages that may metamorphose into epithelioid cells, which are encircled by mononuclear leukocytes, forming granulomas. (medscape.com)
  • Most tissues harbor resident mononuclear phagocytes, that is, dendritic cells and macrophages. (hindawi.com)
  • Mononuclear phagocytes are a group of phenotypic distinct members, often referred to as either macrophages or dendritic cells (DC), that derive from myeloid precursors and that contribute to the functions of peripheral tissues [ 1 ]. (hindawi.com)
  • In this paper we apply the tissue-centric perspective to discuss the role of resident and infiltrating macrophages and dendritic cells in different organs. (hindawi.com)
  • All solid organs and most other tissues harbor a network of DC or macrophages (Table 1 ). (hindawi.com)
  • The latter refers to a special kind of inflamma tion that's characterised by the presence of activated macrophages (epithelioid cells) [url=https://archives.gov.pk/rx-pharmacy/order-dapsone-online-no-rx/] skin care 101 tips dapsone 100mg cheap mastercard[/url]. (ehd.org)
  • Skin healing results after implantation of adult stem cells derived from adipose tissue were satisfactory. (rbcp.org.br)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • The research themes rank around molecular mechanisms matrix proteins and fibers exert in disorders affecting blood vessels, bone, skin and adipose tissue. (mcgill.ca)
  • Despite improved surgical techniques, the tendon-to-bone healing rate is unsatisfactory due to difficulties in restoring the delicate transitional tissue between bone and tendon. (wjgnet.com)
  • MSCs have been isolated from various tissues such as bone marrow (BM), umbilical cord blood, skeletal muscle and brain. (biomedcentral.com)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
  • Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
  • Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
  • An immunologically identical cardiac tissue can be produced by direct reprogramming from the patients' tissue without the potential risk of tumorigenesis that occurs with induced pluripotent stem cells [ 2 ] and the ethical issue using embryonic stem cells. (thno.org)
  • Embryonic stem cells (ESC) can be expanded indefinitely without undergoing replicative senescence or aging due to their high telomerase expression. (biomedcentral.com)
  • The blood and lymphatic vascular systems are essential for the efficient transport of oxygen, nutrients, signaling molecules, and leukocytes to and from peripheral tissues, the removal of waste products, and the preservation of fluid homeostasis. (nature.com)
  • 2022). Fibrillin-1 regulates white adipose tissue development, homeostasis, and function. (mcgill.ca)
  • A classification that sufficiently covers their phenotypic heterogeneity and plasticity during homeostasis and disease does not yet exist because cell culture-based phenotypes often do not match those found in vivo . (hindawi.com)
  • A tissue-centric view assumes that changing tissue microenvironments shape the phenotypes of their resident and infiltrating mononuclear phagocytes to fulfill the tissue's need to maintain or regain homeostasis. (hindawi.com)
  • Thus, organ- and disease phase-specific microenvironments determine macrophage and dendritic cell heterogeneity in a temporal and spatial manner, which assures their support to maintain and regain homeostasis in whatever condition. (hindawi.com)
  • A more tissue-centric view of these processes, claiming that the tissues define phenotype and function of resident and infiltrating immune cells to meet tissues needs during homeostasis and disease, seems provocative [ 4 , 5 ]. (hindawi.com)
  • We examine tissue needs to maintain homeostasis and how to regain homeostasis upon tissue injury. (hindawi.com)
  • These cells provide several important physiological functions during homeostasis (Figure 1 ). (hindawi.com)
  • Diseases associated with defects in adipocyte homeostasis, such as lipodystrophy and focal dermal hypoplasia, lead to alopecia. (jcadonline.com)
  • Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)
  • The culmination of these biological processes results in the replacement of normal skin structures with fibroblastic mediated scar tissue. (medscape.com)
  • In conclusion, the present data confirm and extend (dWAT layer) the data related to the presence of differences between men and women in the skin, particularly in scar tissues, which may contribute to the more effective and gender-tailored improvement of skin care interventions. (bvsalud.org)
  • However, the presence of excess numbers of myofibroblasts in wounded tissue is undesirable because of the risk of fibrotic scar formation. (molvis.org)
  • Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
  • This paper provides insight and an overview of the QA/QC aspect during MyDerm ® manufacturing in a GMP-compliant facility in the Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Universiti Kebangsaan Malaysia. (biomedcentral.com)
  • Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
  • In this review, we analyze the therapeutic agents that have been used for AGA and emphasize the potential of new therapies based on advances in stem cell technologies and regenerative medicine. (benitonovas.com)
  • Adipose-derived stem cells (ASCs) from human and animal fat have emerged as therapeutic alternatives for damaged tissues. (bvsalud.org)
  • The aim of this paper is to discuss the general overview of the quality assurance and quality control implemented in the manufacturing of cell and tissue product, with emphasis on our experience in the manufacturing of MyDerm ® , an autologous bilayered human skin substitute. (biomedcentral.com)
  • 11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)
  • 24 - 26 ECs may provide signals for BMP receptor activation in DPCs similar to those signals that promote survival of MSCs in human embryoid bodies composed of multipotent cells. (benitonovas.com)
  • Here, we developed CellAgeClock, a new epigenetic clock that measures subtle ageing changes in primary human cells in vitro . (biorxiv.org)
  • The CellAgeClock outperforms other epigenetic clocks in measuring subtle ageing changes in primary human cells in culture. (biorxiv.org)
  • Our method expands the scope of CpG methylation profiling from measuring human chronological and biological age from human samples in years, to accurately and rapidly detecting anti-ageing potential of drugs using human cells in vitro , providing a novel accelerated discovery platform to test sought after geroprotectors. (biorxiv.org)
  • Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
  • Senescent cells accumulate progressively through life in a variety of mammalian species [ 15 ], and premature senescence is a hallmark of many human progeroid syndromes. (beds.ac.uk)
  • Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells. (thermofisher.com)
  • Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)
  • They can acquire an M1 or M2 phenotype to promote or suppress an inflammatory response, thereby influencing other cells' activity via the production of pro-inflammatory or anti-inflammatory cytokines. (wikipedia.org)
  • [ 2 ] which has also been denoted as inflammatory, proliferation, and remodeling-and this is maintained by some authors. (medscape.com)
  • We found that MSC-Exo greatly reduced the intensity of ongoing EAU as their parent cells by reducing the infiltration of T cell subsets, and other inflammatory cells, in the eyes. (nature.com)
  • It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
  • Herein, the growth and proliferation, gene expression, multipotency and phenotype of cultured hBMSCs were compared with those incubated with CTX. (ijbs.com)
  • Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. (thermofisher.com)
  • Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
  • Rabbit ASCs were isolated and expanded in vitro with relative abundance, cells expressed typical surface markers (CD49e, CD90 and CD29). (biomedcentral.com)
  • These epidermal and connective tissue layers protect against mechanical and chemical harm and repel pathogenic invaders [ 19 ]. (biomedcentral.com)
  • Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
  • These lesions often replace the dermal papillae, but they can also extend into the deeper connective tissue. (medscape.com)
  • The critical effects of acute duration iodomethane exposure are: (1) fetal losses in rabbits, (2) lesions in rat nasal epithelium, and (3) transient neurotoxicity in rats. (cdc.gov)
  • Platelet-derived growth factor (PDGF) signalling is essential for many key cellular processes in mesenchymal cells. (biomedcentral.com)
  • 1991), and the original name for the temporary organ of repair, 'granulation tissue' (Hunter, 1787), is derived from the prominence of its vessels. (docksci.com)
  • However, their antigen-presenting ability is relatively lower than dendritic cells (DCs) and Langerhans cells (LCs) in the skin. (wikipedia.org)
  • The mammalian skin consists of three main layers: the epidermis, the dermis, and the subcutaneous tissue, including the fascia (Fig. 1 ). (biomedcentral.com)
  • Adipose cells organized in small clusters under the reticular dermis closely interact with hair follicular cells and regulate the hair cycle. (jcadonline.com)
  • Denatured dermis caused by deep skin burns is strongly associated with functional impairment, cell metabolism disorders and pathologically morphological changes. (molcells.org)
  • Our results highlight the remarkablepotential use of BC-Ppy scaffolds as a cardiac patch in tissue regenerativetherapies. (ibecbarcelona.eu)
  • Nano-thin and nano-porous membranes and heart like electric stimulus were used in the cardiac-mimetic cell-culture system. (thno.org)
  • 27 Together, dermal blood vessels and DPCs orchestrate a suitable microenvironment for the growth and survival of HFSCs. (benitonovas.com)
  • Mesenchymal stem cell (MSC)-based therapy for the treatment of autoimmune diseases has demonstrated a particular promise after its successful applications in both animal models and patients. (nature.com)
  • In recent years, the development in cell therapy and stem cell research has led scientist to engage in the production of cells and tissue product that is of clinical grade. (biomedcentral.com)
  • Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
  • Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
  • Stem Cell Res. (sciendo.com)
  • check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)
  • Ageing is the gradual decline in organismal fitness that occurs over time leading to tissue dysfunction and disease. (elifesciences.org)
  • The accumulation of senescent cells also occurs in vivo with advancing age and causes much degenerative age-related pathology. (beds.ac.uk)
  • Fetal infection occurs transplacentally during the maternal viremic phase, but the mechanisms by which rubella virus causes fetal damage are poorly understood. (medscape.com)
  • In conclusion, our results provide a platform in which xenograft vessels are decellularized properly in a short time, and the recellularization process is significantly improved after the bioconjugation of the acellular scaffold with apelin-13 in terms of cell adhesion and viability within the scaffold. (biomedcentral.com)
  • CD326 mediates calcium-independent, homophilic, cell-cell adhesion and may function as a growth factor receptor. (thermofisher.com)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • The main property that sets ESC apart from other cells is their pluripotent nature, meaning they can give rise/differentiate to cells of the three primary germ layers (mesoderm, endoderm and ectoderm) [ 13 ]. (biomedcentral.com)
  • Biologic factors that include signaling from stem cells, dermal papilla cells, and platelet-rich plasma are some of the current therapeutic agents being studied for hair restoration with milder side effects. (benitonovas.com)
  • This slightly different perspective may somewhat shape our understanding of macrophage heterogeneity and tissue pathology but certainly also raise new questions for future research. (hindawi.com)
  • Due to their considerable plasticity and heterogeneity, the tissue-based DC and macrophage populations have been defined as mononuclear phagocytes [ 1 , 6 , 7 ]. (hindawi.com)
  • To this end, multiple tissue-resident cells and recruited immune cells cooperate to efficiently repair the injured tissue. (biomedcentral.com)
  • This function of the cutaneous system is mediated by a myriad of highly specialized immune cells. (biomedcentral.com)
  • In interaction with other non-immune cells, such immune cell subsets ensure the functionality and integrity of the skin layers [ 1 ]. (biomedcentral.com)
  • To this end and within their broad functionality and operationality, Tregs are able to adopt unique features of skin cells and contribute to the skin's role as an immune barrier. (biomedcentral.com)
  • An immune-centric view assumes that a particular priming of phagocytes then causes a particular type of pathology in target tissues, conceptually similar to antigen-specific T-cell priming. (hindawi.com)
  • It functions by slowing or blocking cell growth and compromising the immune system's response. (ijbs.com)
  • Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
  • Metabolism of tumor cells is characterised by irregular enhance in anaerobic glycolysis, no matter excessive oxygen focus, leading to a major accumulation of power from glucose sources. (aabioetica.org)
  • Motor neuron-like NSC-34 cells as a new model for the study of vitamin D metabolism in the brain. (uu.se)
  • SEM images and characterization methods were also used to evaluate the effect of apelin-13 attachment to the acellular scaffold on tissue recellularization. (biomedcentral.com)
  • We also developed a novel strategy to eliminate the remnant detergents from the scaffold and increase cell viability by incubating acellular scaffolds with Bio-Beads SM-2 resin. (biomedcentral.com)
  • It is highly recommended to perform cell manufacturing for therapeutic use in a well-designed GMP-compliant cleanroom facility. (biomedcentral.com)
  • Histopathology of peripheral nerve and sympathetic ganglion from a patient with autonomic failure, oat-cell carcinoma of the lung, and positive anti-HU antibody titer. (medscape.com)
  • The TGF-β family forms an important group of growth factors, consisting of three isoforms in man, and is important for matrix deposition because it modulates fibroblast recruitment and proliferation. (biomedcentral.com)
  • This growth factor also stimulates production of collagens, proteoglycans, elastin, fibronectin, tenascin and thrombospondin, diminishes production of extracellularly active neutral endoproteinases belonging to the matrix metalloproteinase (MMP) and serine proteinase families, and stimulates production of endogenous MMP inhibitors (tissue inhibitor of metallo-proteinase [TIMP]) and serpins (plasminogen activator inhibitor-1). (biomedcentral.com)
  • We previously demonstrated that mesenchymal stem cells (MSCs) ameliorated experimental autoimmune uveoretinitis (EAU) in rats. (nature.com)
  • Our group recently reported that CD73 on the cell membrane also contributes to the immunomodulatory capacity of MSCs 3 , suggesting that MSCs exert their immunomodulatory properties in a multifactorial manner. (nature.com)
  • When an adequate number of cells (ASCs 10 × 10 6 and BM-MSCs 3 × 10 6 , because of their low rate of proliferation) was reached, two skin wounds were surgically induced in each animal. (biomedcentral.com)
  • 4 HFSCs are located in the bulge region of the follicle and they interact with mesenchymal SCs (MSCs) located in the dermal papilla (DP). (benitonovas.com)
  • Prevention of bacterial contamination is even more important in the case of gingival graft surgery because a significant amount of tissue is lost at a palatal donor site. (spandidos-publications.com)
  • Autogenous gingival grafts are often accompanied by discomfort, pain and retarded tissue repair depending on a patient's condition. (spandidos-publications.com)