• Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
  • First, the fin margin is covered by a wound epithelium, and next a proliferative blastema arises by dedifferentiation of stump tissues near the amputation. (unifr.ch)
  • Just as plant cells respond to injury by undergoing callus formation via dedifferentiation, some animal models dedifferentiate their cells to form blastema, which are analogous to plant calluses, after limb amputation. (wikipedia.org)
  • N1 transgenic hindlimbs, which do not regenerate, do not form an apical epithelial cap or cone shaped blastema following amputation. (biomedcentral.com)
  • Furthermore, we identified that chromatin modification is critical for redifferentiation of blastema cells. (unifr.ch)
  • Balanced cell proliferation and redifferentiation along with cell movements then progressively reestablish patterned tissues and restore the fin to its original size and shape. (bvsalud.org)
  • The loss of specialization observed in dedifferentiation can be noted through changes in gene expression, physiology, function witin the organism, proliferative activity, or morphology. (wikipedia.org)
  • In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
  • Which biological mechanisms guide the mature cells through the regeneration process? (unifr.ch)
  • We are currently investigating which adhesion mechanisms organize the different cells types during fin regeneration. (unifr.ch)
  • The pathways discussed below are found to be closely related to dedifferentiation and regeneration in some species. (wikipedia.org)
  • Because not one pathway has been elucidated as necessary for all dedifferentiation and regeneration, it is possible that the mechanism functions differently in different species. (wikipedia.org)
  • The BMP pathway has been shown to be necessary for dedifferentiation and regeneration in tadpoles. (wikipedia.org)
  • Lowered Notch1 expression resulted in no tadpole tail regeneration, and induced Notch1 expression was able to partially rescue tail regeneration in the form of notochord and spinal cord (but very little musculature. (wikipedia.org)
  • Epimorphic regeneration is the process by which complete regeneration of a complex structure such as a limb occurs through production of a proliferating blastema. (biomedcentral.com)
  • Furthermore, the underlying mesenchyme remains rounded and does not expand to form a cone shaped blastema, a normal feature of successful regeneration. (biomedcentral.com)
  • Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
  • We have developed a heat shock inducible transgenic line ( N1 ) of Xenopus in which the BMP antagonist Noggin can be induced at a specific time during either development or regeneration, repressing BMP signalling [ 10 , 12 ]. (biomedcentral.com)
  • We have used this line to show that BMP function is not only required for appendage regeneration but that it is specifically needed to generate a proliferating blastema while being dispensable for wound healing [ 12 ]. (biomedcentral.com)
  • In this study, the level of proenkephalin-A (PENK-A), expressed by renal proximal tubular epithelial cells, decreases significantly with the loss of renal proximal tubules and increased at the termination phase of zebrafish kidney regeneration. (bvsalud.org)
  • To determine fibroblast plasticity in vivo, we develop a laser-induced tendon ablation and regeneration model. (bvsalud.org)
  • Active Hedgehog (Hh) signaling is required for the proliferation of activated fibroblasts to ensure efficient tenocyte regeneration. (bvsalud.org)
  • CKIα ablation induces p53 activation, and CKIα degradation underlies the therapeutic effect of lenalidomide in a pre-leukemia syndrome. (regenerativemedicine.net)
  • While Manning's research was in reference to plants, it helped establish the foundation for our modern day understanding of dedifferentiation and cell plasticity. (wikipedia.org)
  • Our experiments revealed that the myocardium adjacent to the injury undergoes dedifferentiation, during which embryonic cardiac programs become reactivated to give rise to new tissue. (unifr.ch)
  • Several years ago, our laboratory established a cryoinjury-induced myocardial infarction model in zebrafish, whereby a freezing-thawing procedure destroys approx. (unifr.ch)
  • Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
  • Using single-cell RNA sequencing, we uncover distinct sclerotome-derived fibroblast populations in zebrafish, including progenitor-like perivascular/interstitial fibroblasts, and specialized fibroblasts such as tenocytes. (bvsalud.org)
  • While it can be induced in a laboratory setting through processes like direct reprogramming and the production of induced pluripotent stem cells, endogenous dedifferentiation processes also exist as a component of wound healing mechanisms. (wikipedia.org)
  • Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
  • Fin injury triggers wound epidermis formation and the dedifferentiation of injury-adjacent mature cells to establish an organized blastema of progenitor cells. (bvsalud.org)
  • Our laboratory identified signalling pathways that orchestrate proliferation, migration and patterning of different tissues in the regenerating fin. (unifr.ch)
  • Dedifferentiation would be represented by the marble moving uphill through the pathways it has already taken, until it settles somewhere above the most downhill location. (wikipedia.org)
  • Some of the pathways that have shown interaction in dedifferentiation are MSX1, Notch 1, BMP, and Wnt/β-Catenin. (wikipedia.org)
  • Our fate mapping experiments demonstrated that the activated mesenchyme of the stump gives rise to the blastema. (unifr.ch)
  • Furthermore, H2O2 influences the expression of tcf21, a vital factor in the formation of renal progenitor cell aggregates, by remodeling H3K4me3 in renal cells. (bvsalud.org)
  • This is in contrast to differentiation, where differences in gene expression, morphology, or physiology arise in a cell, making its function increasingly specialized. (wikipedia.org)
  • The mechanism by which dedifferentiation occurs has not been completely illuminated. (wikipedia.org)
  • Dedifferentiation (pronounced dē-ˌdi-fə-ˌren-chē-ˈā-shən) is a transient process by which cells become less specialized and return to an earlier cell state within the same lineage. (wikipedia.org)
  • Some claim that dedifferentiation is strictly limited to the same cell lineage from which it is derived. (wikipedia.org)
  • This property involves activation of the remaining tissue at the site of injury to promote cell division, cell migration and replacement of the missing structures. (unifr.ch)
  • However, the markers selected to represent dedifferentiation can differ according to tissue and cell types that are being studied. (wikipedia.org)
  • mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
  • Her lab established the cryoinjury model system for inducing myocardial infarction in adult fish. (unifr.ch)
  • We are currently investigating the regenerative capacity of specific cardiac cell populations and comparing the restorative programs of different fish species. (unifr.ch)
  • By combining single-cell clonal analysis and live imaging, we demonstrate that perivascular/interstitial fibroblasts actively migrate to the injury site, where they proliferate and give rise to new tenocytes. (bvsalud.org)
  • In our modern day understanding of dedifferentiation, some controversies remain when defining the boundaries of its definition. (wikipedia.org)
  • We identified multiple newly gained SEs in primary mouse acute myeloid leukemia (AML) cells and demonstrate that the inhibitors abolish many SEs and preferentially suppress the transcription elongation of SE-driven oncogenes. (regenerativemedicine.net)
  • This repressor would be able to keep cells undifferentiated during development. (wikipedia.org)
  • Here we describe the development of CKIα inhibitors, which co-target the transcriptional kinases CDK7 and CDK9, thereby augmenting CKIα-induced p53 activation and its anti-leukemic activity. (regenerativemedicine.net)
  • A mechanistic understanding of these coordinated cell behaviors and transitions requires direct knowledge of proteins in their physiological context, including expression, subcellular localization, and activity. (bvsalud.org)
  • This suggests an increase in cell potency, meaning that, following dedifferentiation, a cell may possess the ability to re-differentiate into more cell types than it did prior to dedifferentiation. (wikipedia.org)
  • In the 1940s C. H. Waddington created the "Epigenetic Landscape", a diagrammatic representation of cell fate from less differentiated to more differentiated cell types. (wikipedia.org)