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  • mouse
  • Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC. (merckmillipore.com)
  • Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. (gentaur.com)
  • Receptors
  • These memory T cells lack lymph node-homing receptors and are thus found in the peripheral circulation and tissues. (wikipedia.org)
  • ImmTACs (Immune mobilising monoclonal T-cell receptors Against Cancer) are a class of bispecific biological drug being investigated for the treatment of cancer and viral infections which combines engineered cancer-recognizing TCRs with immune activating complexes. (wikipedia.org)
  • Naturally occurring TCRs are low affinity (0.18-387 micromolar range) 2-chain membrane receptors expressed on the surface of T cells. (wikipedia.org)
  • Glucocorticoids also suppress the humoral immunity, causing B cells to express smaller amounts of IL-2 and IL-2 receptors. (wikipedia.org)
  • The sphingosine-1-phosphate receptors regulate fundamental biological processes such as cell proliferation, angiogenesis, migration, cytoskeleton organization, endothelial cell chemotaxis, immune cell trafficking and mitogenesis. (wikipedia.org)
  • Sphingosine-1-phosphate receptors are also involved in immune-modulation and directly involved in suppression of innate immune responses from T cells. (wikipedia.org)
  • protein
  • To address this, in the present study, two TCC cell lines, T24 and UM‑UC‑3, were treated with cholera toxin [protein kinase A (PKA) activator] and KT5720 (PKA inhibitor). (spandidos-publications.com)
  • Combined with D-type (D1, D2, D3) cyclin, Cdk4/6 is activated and phosphorylates the retinoblastoma protein (Rb), promoting the escape of E2F from phosphorylated Rb, and therefore inducing cell transit from the G1 phase into the S phase. (spandidos-publications.com)
  • It has been demonstrated that the mitogen-activated protein kinase (MAPK) cascade (Ras/Raf/Mek/Erk), which induces cyclin and Cdk gene expression and promotes cell cycle progression by activating upstream transcription factors ( 7 ), is responsible for TCC oncogenesis and tumor growth ( 8 ). (spandidos-publications.com)
  • IMP321 is a 200 kDA soluble dimeric recombinant fusion protein of the extracellular portion of LAG3 with immunoglobulin, designed to activate antigen presenting cells. (wikipedia.org)
  • had successfully produced a soluble fusion protein of LAG3 and immunoglobulin around 1995 and had initially envisaged its use as an immunosuppressant after evidence that its interaction with MHC class II molecules leads to the down-regulation of CD4+ antigen-specific T cell clone proliferation and cytokine secretion, and that it inhibited the alloresponses of naive peripheral blood lymphocytes. (wikipedia.org)
  • From many investigations, regucalcin has been shown to play a multifunctional role in many cell types as a regulatory protein in the intracellular signaling system. (wikipedia.org)
  • Regucalcin is greatly expressed in the liver of rats, although the protein is found in small amounts in other tissues and cells. (wikipedia.org)
  • Also, regucalcin has been demonstrated to transport into the nucleus of cells, and it can inhibit nuclear protein kinase, protein phosphatase, and deoxyribonucleic acid and ribonucleic acid synthesis. (wikipedia.org)
  • Moreover, regucalcin has been shown to have an inhibitory effect on aminoacyl t-RNA synthetase, a rate limiting enzyme at translational process of protein synthesis and an activatory effect on cystein protease and superoxide dismutase in liver and kidney cells. (wikipedia.org)
  • Regucalcin plays a pivotal role as a suppressor protein for cell signaling systems in many cell types. (wikipedia.org)
  • Plasma cell dyscrasias (also termed plasma cell disorders and plasma cell proliferative diseases) are a spectrum of progressively more severe monoclonal gammopathies in which a clone or multiple clones of pre-malignant or malignant plasma cells (sometimes in association with lymphoplasmacytoid cells or B lymphocytes) over-produce and secrete into the blood stream a myeloma protein, i.e. an abnormal monoclonal antibody or portion thereof. (wikipedia.org)
  • At the other end of this spectrum, detection of the myeloid protein indicates the presence of a hematological malignancy, i.e. multiple myeloma, Waldenström's macroglobulinemia, or other B cell-associated neoplasm, that derives stepwise from its MGUS precursors. (wikipedia.org)
  • This suggests that cell division may be regulated in part by dilution of Wee1 protein in cells as they grow larger. (wikipedia.org)
  • The protein kinase Cdr2 (which negatively regulates Wee1) and the Cdr2-related kinase Cdr1 (which directly phosphorylates and inhibits Wee1 in vitro) are localized to a band of cortical nodes in the middle of interphase cells. (wikipedia.org)
  • The cell polarity protein kinase Pom1, a member of the dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) family of kinases, localizes to cell ends. (wikipedia.org)
  • Specification of primordial germ cells in the laboratory mouse is initiated by high levels of bone morphogenetic protein (BMP) signaling, which activates expression of the transcription factors Blimp-1/Prdm1 and Prdm14. (wikipedia.org)
  • Following the identification of a Pur factor, which specifically bound a purine-rich sequence in the control region of the c-MYC gene, the gene, PURA, encoding the protein, Pur-alpha, was cloned and sequenced for both human and mouse (GenBank U02098.1). (wikipedia.org)
  • Several cell cycle regulatory functions may be mediated by Pur-alpha binding to Cyclin/Cdk protein kinases, which phosphorylate proteins regulating cell cycle transition points. (wikipedia.org)
  • These cell cycle effects are consistent with an interaction between Pur-alpha and CDK, cell cycle-dependent protein kinases. (wikipedia.org)
  • These genetic abnormalities effect the Wnt signaling pathway, regulation of the cell cycle, RNA metabolism, protein folding, and cadherin-related cell adherence to extracellular matrix. (wikipedia.org)
  • Overexpression of RGPR-p117 did not cause an alteration of cell proliferation and led to significant decreases in protein and DNA contents in cloned normal rat kidney proximal tubular epithelial NRK52E cells. (wikipedia.org)
  • proteins
  • Western blot analysis was used to detect the expression of proteins involved in cell cycle regulation. (spandidos-publications.com)
  • β-catenin and γ-catenin (plakoglobin) interact directly with E-cadherin's COOH-terminal domain in a mutually exclusive way, and both proteins associate with α-catenin, which links the cadherin complexes to the actin cytoskeleton and mediates stable cell adhesion. (rupress.org)
  • The most common cause of kidney failure in multiple myeloma is due to proteins secreted by the malignant cells. (wikipedia.org)
  • Myeloma cells produce monoclonal proteins of varying types, most commonly immunoglobulins (antibodies) and free light chains, resulting in abnormally high levels of these proteins in the blood. (wikipedia.org)
  • but, whereas antibodies are restricted to targeting cell surface or secreted proteins TCRs can recognise peptides derived from intracellular targets presented by human leukocyte antigen (HLA). (wikipedia.org)
  • The overproduced Ig's, termed myeloma proteins, commonly circulate in blood, may accumulate in urine, and are the hallmarks of plasma cell dyscrasias including their most malignant forms viz. (wikipedia.org)
  • Endodermal cells differentiate and together with Wunen proteins they induce the migration through the gut. (wikipedia.org)
  • Wunen proteins are chemorepellents that lead the germ cells away from the endoderm and into the mesoderm. (wikipedia.org)
  • Phosphorylated RGPR-p117 specifically binds to the TTGGC motif in the promoter region of various genes to enhance the gene expression of various proteins, and plays a crucial role as a transcription factor in the cells. (wikipedia.org)
  • epithelial cells
  • β-Catenin is essential for E-cadherin-mediated cell adhesion in epithelial cells, but it also forms nuclear complexes with high mobility group transcription factors. (rupress.org)
  • Using a mouse mammary epithelial cell system, we have shown previously that conversion of epithelial cells to a fibroblastoid phenotype (epithelial-mesenchymal transition) involves downregulation of E-cadherin and upregulation of β-catenin transcriptional activity. (rupress.org)
  • Regucalcin plays a role in the promotion of urinary calcium transport in the epithelial cells of kidney cortex. (wikipedia.org)
  • Voltage-gated hydrogen channel 1 is a voltage-gated proton channel that has been shown to allow proton transport into phagosomes and out of many types of cells including spermatozoa, electrically excitable cells and respiratory epithelial cells. (wikipedia.org)
  • Endoplasmic
  • Endoplasmic reticulum (ER) stress in insulinoma cells increased cytosolic calcium and the activity of tissue transglutaminase 2 (tTG2). (diabetesjournals.org)
  • Regucalcin plays a pivotal role in the keep of intracellular Ca2+ homeostasis due to activating Ca2+ pump enzymes in the plasma membrane (basolateral membrane), microsomes (endoplasmic reticulum) and mitochondria of many cells. (wikipedia.org)
  • cytokines
  • These cells can differentiate into one of several subtypes, including T H 1 , T H 2 , T H 3 , T H 17 , T H 9 , or T FH , which secrete different cytokines to facilitate different types of immune responses. (wikipedia.org)
  • An April 2006 paper in Cancer Research showed, in vitro, that IMP321 would induce an antigen-specific CD8+ T-cell response in human PBMCs, with the T cells thus created being cytotoxic and capable of producing Tc1 cytokines. (wikipedia.org)
  • monoclonal
  • Anti-gp340 Monoclonal Antibody Description: 100 g Research Focus: cancer & cell proliferation Storage: -20C Shipping Temperature: 4C. (bio-medicine.org)
  • Although the total immunoglobulin level is typically elevated in multiple myeloma, the majority of the antibodies are ineffective monoclonal antibodies from the clonal plasma cell. (wikipedia.org)
  • Secondary plasma cell leukemia (sPCL) results from the comparatively slow development of plasma cell/plasma cell precursor genetic abnormalities which initially create a clone of cells that cause the premalignant condition of monoclonal gammopathy of undetermined significance. (wikipedia.org)
  • cycle
  • It is known that patients with bladder cancer have mutations in cell cycle checkpoint genes that are associated with malignant growth and genetic instability. (spandidos-publications.com)
  • The alteration of multiple cell cycle regulators renders patients with high-grade TCC with a survival rate of only 8% ( 3 ). (spandidos-publications.com)
  • Cell cycle progression is governed by different cyclin-dependent kinases (Cdks) in a step-wise manner, including Cdk4/6 during the G1 phase and Cdk2 from the late G1 phase to the early S phase. (spandidos-publications.com)
  • Moreover, based on cell cycle phase distribution and growth curve, most A549 and H446 cells may not be stem cells, but they acquired stem cell characteristics when they were alone. (biomedsearch.com)
  • Furthermore, coexpression of β-catenin or lymphoid enhancer binding factor-1 or T cell factor 3 with E-cadherin rescued β-catenin transcriptional activity and counteracted E-cadherin-mediated cell cycle arrest. (rupress.org)
  • This is the first report of rescue of retinal proliferation in a microphthalmia model by deletion of a cell cycle regulatory gene. (biologists.org)
  • Work on the topic generally requires an organism whose cell cycle is well-characterized. (wikipedia.org)
  • The end-products of the germ cell cycle are the egg or sperm. (wikipedia.org)
  • dendritic
  • LAG3 is expressed on various cells in the immune system including activated T cells, Natural Killer cells (NK cells), B cells and dendritic cells, being a ligand for MHC class II molecules. (wikipedia.org)
  • However, on dendritic cells LAG-3 is an activator, causing increased antigen presentation to cytotoxic CD8+ T cells when it binds to MHC Class II. (wikipedia.org)
  • Soluble LAG3 was first established as a dendritic cell activator in the late 1990s. (wikipedia.org)
  • However, in March 1999 a key paper in the Journal of Immunology from scientists at the University of Montreal, on which Triebel was listed as a co-author, demonstrated soluble LAG3's role as a dendritic cell activator. (wikipedia.org)
  • A February 2003 paper in the journal Vaccine showed that, in human immature monocyte-derived dendritic cells, IMP321 could induce production of the chemokines that would direct the migration of maturing dendritic cells to lymph nodes. (wikipedia.org)
  • Notably, LAG-3-matured dendritic cells were upregulated for CCR7. (wikipedia.org)
  • Later, the same authors showed that soluble LAG-3 would reduce the differentiation of macrophages and dendritic cells from monocytes, suggested that the positive effect of LAG-3 as a dendritic cell activator related to existing dendritic cells. (wikipedia.org)
  • A March 2006 online paper in Vaccine showed, in animal models, that IMP321 could immunopotentiate therapeutic vaccines by inducing dendritic cell maturation. (wikipedia.org)
  • genes
  • Some features of the B cell repertoire remain stable with age, but elderly subjects show increased numbers of B cells with long CDR3 regions, a trend toward accumulation of more highly mutated IgM and IgG Ig genes, and persistent clonal B cell populations in the blood. (jimmunol.org)
  • Seropositivity for CMV or EBV infection alters B cell repertoires, regardless of the individual's age: EBV infection correlates with the presence of persistent clonal B cell expansions, whereas CMV infection correlates with the proportion of highly mutated Ab genes. (jimmunol.org)
  • Specific to TRMs are genes involved in lipid metabolism, being highly active, roughly 20- to 30-fold more active than in other types of T-cells. (wikipedia.org)
  • The second way is found in birds and mammals, where germ cells are not specified by such determinants but by signals controlled by zygotic genes. (wikipedia.org)
  • The World Health Organization classifies these disorders into a) Myeloid and lymphoid neoplasms with eosinophilia and abnormalities of PDGFRA, PDGFRB, or FGFR1 (i.e. high eosinophil blood counts caused by mutations in the eosinophil cell line of one of these three genes), 'b) Chronic eosinophilic leukemia, and c) the Idiopathic hypereosinophiic syndrome. (wikipedia.org)
  • Clonal hypereosinophilia is hypereosinophilia caused by a pre-malignant or malignant clone of eosinophils that bear mutations in genes for PDGFRA, PDGFRB, or FGFR1 or, alternatively, a chromosome translocation that creates the PCM1-JAK2 fusion gene. (wikipedia.org)
  • survival
  • The fundamental driver of these hypereosinophilic (or uncommonly eosinophilic) disorders is the mutation which increases the proliferation, survival, and further mutation of cells descendant from the originally mutated cell. (wikipedia.org)
  • migration
  • After transport, involving passive movements and active migration, germ cells arrive at the developing gonads. (wikipedia.org)
  • Migration starts with 3-4 cells that undergo three rounds of cell division so that about 30 PGCs arrive at the gonads. (wikipedia.org)
  • plasma cell dyscrasia
  • Plasma cell leukemia (PCL) is a plasma cell dyscrasia, i.e. a disease involving the malignant degeneration of a subtype of white blood cells called plasma cells. (wikipedia.org)
  • PCL may present as primary plasma cell leukemia, i.e. in patients without prior history of a plasma cell dyscrasia or as secondary plasma cell dyscrasia, i.e. in patients previously diagnosed with a history of its predecessor dyscrasia, multiple myeloma. (wikipedia.org)
  • myeloma
  • Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized (BALB/c X C57BL/6)F1 (CbyB6F1) lymphokine-activated killer (LAK) cells and mouse myeloma cellline. (bio-medicine.org)
  • Multiple myeloma, also known as plasma cell myeloma, is a cancer of plasma cells, a type of white blood cell normally responsible for producing antibodies. (wikipedia.org)
  • In a very small percentage of these cases, the progressive development of further genetic abnormalities serially create a clone(s) of plasma cells that cause the more serious but still premalignant disorder of smoldering multiple myeloma, overt myeloma cancer, and ultimately sPCL. (wikipedia.org)
  • mechanism
  • Nevertheless, the underlying mechanism of CDA gene silencing in the biological processes of K562 cells remains to be clarified. (springer.com)
  • We have focused on the mechanism of proliferation control in the mammalian neural retina because cell proliferation is a primary determinant of retinal size and cell number. (biologists.org)
  • The underlying mechanism involves abnormal plasma cells producing abnormal antibodies which can cause kidney problems and overly thick blood. (wikipedia.org)
  • ImmTACs excert their activity through T cell redirection, a mechanism of action used by several other bi-specific biologics such as the Bi-specific T cell enagers (BiTEs). (wikipedia.org)
  • For some cells, there is a mechanism by which cell division is not initiated until a cell has reached a certain size. (wikipedia.org)
  • populations
  • Advanced age has been reported to lead to increased or decreased B cell counts in the peripheral blood, increased, decreased or unchanged proportions of naive B cells, and increased CD5 + B cell populations ( 3 , 5 , 9 - 13 ). (jimmunol.org)
  • Although most information is currently based on observations in the cytotoxic T cells (CD8-positive) subset, similar populations appear to exist for both the helper T cells (CD4-positive) and the cytotoxic T cells. (wikipedia.org)
  • Cell populations go through a particular type of exponential growth called doubling. (wikipedia.org)
  • After splitting into two populations, the germ cells continue migrating laterally and in parallel until they reach the gonads. (wikipedia.org)
  • The germ cells split into two populations and move to the paired gonadal ridges. (wikipedia.org)
  • peripheral
  • Patients with type 1 diabetes had elevated frequencies of T cells specific for these epitopes in their peripheral blood. (diabetesjournals.org)
  • Thus, although this population as a whole is abundant within the peripheral circulation, individual virtual memory T cell clones reside at relatively low frequencies. (wikipedia.org)
  • synthesis
  • We show that TCR ligation by conventional peptide Ag is coupled to a burst of PI synthesis in nontransformed T cells, which may represent a rate-limiting step for downstream PI hydrolysis. (jimmunol.org)
  • By contrast, TCR ligation of the same T cell clone by retroviral superantigen leads to similar levels of T cell proliferation without detectable PI synthesis. (jimmunol.org)
  • This diminishes both B cell clone expansion and antibody synthesis. (wikipedia.org)
  • They induce the lipocortin-1 (annexin-1) synthesis, which then binds to cell membranes preventing the phospholipase A2 from coming into contact with its substrate arachidonic acid. (wikipedia.org)
  • differentiate
  • As RPCs become postmitotic and differentiate, Chx10 expression is terminated in all cell types, except bipolar interneurons, which are the last neuronal class to be generated during retinal histogenesis. (biologists.org)
  • This means that they can differentiate in any cell type in the body and thus germ cells. (wikipedia.org)
  • adhesion
  • Since proliferating cells had a higher β-catenin activity than G1 phase-arrested or contact-inhibited cells, we conclude that β-catenin transcriptional activity is essential for cell proliferation and can be controlled by E-cadherin in a cell adhesion-independent manner. (rupress.org)
  • lymphocytes
  • By preventing the clonal expansion of lymphocytes in the induction phase of the immune response, it affects both the cell and the humoral immunity. (wikipedia.org)
  • precursor
  • Primordial germ cells, germ cells that still have to reach the gonads, also known as PGCs, precursor germ cells or gonocytes, divide repeatedly on their migratory route through the gut and into the developing gonads. (wikipedia.org)
  • PCL is caused by the development of an excessively high number of genetic abnormalities in plasma cells or, more particularly, their precursor B cells and plasmablasts (see plasma cells). (wikipedia.org)
  • mammalian
  • In cultured mammalian cells, such a strong enhancement of actin polymerization is induced by overproduction of LIMKs or TESKs (Niwa, 2002 and references therein). (sdbonline.org)
  • Like other areas of the CNS, however, the mammalian neural retina undergoes a massive expansion in cell number by proliferation. (biologists.org)
  • Mammalian eggs are somewhat symmetrical and after the first divisions of the fertilized egg, the produced cells are all totipotent. (wikipedia.org)
  • Differentiation
  • Not only must sufficient numbers of progenitor cells be generated for neuronal and glial differentiation, but the correct cell number ratios among different CNS tissues must also be attained. (biologists.org)
  • significantly
  • The results revealed that cholera toxin significantly induced G1 arrest and downregulated the expression of cyclin D1 and cyclin‑dependent kinase 4/6 in the TCC cell lines, and this was rescued by KT5720. (spandidos-publications.com)
  • Young, 1984 ) do not contribute significantly to the size of the total cell population during retinal development. (biologists.org)
  • Primary hypereosinophilia is due to the development of a clone of eosinophils, i.e. a group of genetically identical eosinophils derived from a significantly mutated ancestor cell. (wikipedia.org)
  • pathogens
  • Humoral responses are critical for responding to pathogens such as Streptococcus pneumoniae and influenza viruses that cause increased morbidity and mortality in the elderly, but age-related changes in human B cells and Ig repertoires are only beginning to be understood ( 6 - 8 ). (jimmunol.org)
  • These cells are thought to play a major role in protective immunity against pathogens. (wikipedia.org)
  • Molecular
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • In the 1990s, Wong-Staal's research focused on gene therapy, using a ribozyme "molecular knife" to repress HIV in stem cells. (wikipedia.org)
  • In the field of molecular biology, nuclear receptors are a class of proteins found within cells that are responsible for sensing steroid and thyroid hormones and certain other molecules. (wikipedia.org)
  • stem
  • Time-lapse phase-contrast (left) and fluorescence (right) movies of human ES cells (H9) grown in Essential 8 medium on vitronectin and transfected with 1 µL Lipofectamine Stem reagent complexed with a GFP-expression construct (500 ng total plasmid DNA). (thermofisher.com)
  • Experience superior transfection in stem cells for yourself. (thermofisher.com)
  • The goal of this study was to illustrate the versatility of Invitrogen Lipofectamine Stem Transfection Reagent, which was developed specifically for stem cells, to co-deliver multiple payloads in addition to large plasmid constructs with high transfection efficiency. (thermofisher.com)
  • Stem cell transfection is a key technique for many researchers, but the sensitivity of stem cells has made them difficult to transfect efficiently using reagent-based protocols. (thermofisher.com)
  • Designed for dramatically improved transfection efficiency, Invitrogen Lipofectamine Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA, RNA and RNP such as Cas9/gRNA complexes into a wide range of stem cells, including pluripotent stem cells, neural stem cells and mesenchymal stem cells (Figure 2) . (thermofisher.com)
  • Lipofectamine Stem reagent offers expanded capabilities for stem cell transfection, making it possible to deliver DNA plasmids in sizes up to 11 kb. (thermofisher.com)
  • All of these attributes make this versatile reagent an ideal addition to your stem cell research tool kit and a readily accessible alternative to electroporation. (thermofisher.com)
  • Lipofectamine Stem Reagent outperforms FuGENE HD Reagent in pluripotent stem cell transfection, delivering both small and large DNA plasmids, mRNA, and Cas9 protein complexes. (thermofisher.com)
  • (A) Human ES (H9) cells or Human Episomal iPSCs were transfected with Lipofectamine Stem Reagent or FuGENE HD Regent according to the accompanying protocol and either a 5 kb or 11 kb DNA plasmid expressing GFP and examined for GFP expression 24 hours posttransfection. (thermofisher.com)
  • In the bottom panel NCRM-iPS cells transfected using Lipofectamine Stem Reagent with a ~10.5 kb plasmid expressing Cas9 and GFP followed by detection via immunocytochemistry. (thermofisher.com)
  • (B) Human ES (H9) cells were transfected with Lipofectamine Stem Reagent or FuGENE HD Reagent according to the protocol with an mRNA expressing GFP. (thermofisher.com)
  • C, left) Lipofectamine Stem Reagent was used to transfect cells with Cas9 mRNA (modified)/ gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified) or Cas9 protein/gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified). (thermofisher.com)
  • It's about stem cells, cloning, bioethics and genetics. (blogspot.se)
  • EF-1 alpha promoter versions of the Tet-On 3G Tetracycline Inducible Expression System provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells. (clontech.com)
  • Other studies showed that lack of Daxx gene caused a higher apoptotic rate in embryonic stem cells. (wikipedia.org)
  • It may also mediate the attachment of stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • i.e., they are multipotential hemopoietic stem cells. (wikipedia.org)
  • Beyond unicellular organisms) Telomerase is active in normal stem cells and most cancer cells, but is normally absent from, or at very low levels in, most somatic cells. (wikipedia.org)
  • Embryonic stem cells express telomerase, which allows them to divide repeatedly and form the individual. (wikipedia.org)
  • In adults, telomerase is highly expressed only in cells that need to divide regularly especially in male sperm cells[citation needed] but also in epidermal cells, in activated T cell and B cell lymphocytes, as well as in certain adult stem cells, but in the great majority of cases somatic cells do not express telomerase. (wikipedia.org)
  • cellular
  • Another cellular enzyme, RNAse L-also induced by interferon action-destroys RNA within the cells to further reduce protein synthesis of both viral and host genes. (wikipedia.org)
  • Cellular senescence is the phenomenon by which normal diploid cells cease to divide. (wikipedia.org)
  • As such, cellular senescence represents a change in "cell state" rather than a cell becoming "aged" as the name confusingly suggests. (wikipedia.org)
  • Consistent with this, telomerase-immortalised cells continued to age (according to the epigenetic clock) without having been treated with any senescence inducers or DNA-damaging agents, re-affirming the independence of the process of epigenetic ageing from telomeres, cellular senescence, and the DNA damage response pathway. (wikipedia.org)
  • Wong-Staal used a type of cellular analysis known as radioimmunoprecipitation in order to detect the presence of KS lesions in cells with varying amounts of the tat protein. (wikipedia.org)
  • The growth factors TGF alpha, TGF beta, and IL2 have all been shown to stimulate c-Fos, and thereby stimulate cellular proliferation via AP-1 activation. (wikipedia.org)
  • mRNA
  • Transforming growth factor-ß regulates steady state PTH/PTHrP receptor mRNA levels and PTHrP binding in ROS 17/2.8 osteosarcoma cells. (dentisty.org)
  • Overexpression of p120 leads to malignant transformation of 3T3 cells while treatment with antisense p120 mRNA causes the transformed cells to revert to their original non-malignant phenotype. (wikipedia.org)
  • tumors
  • this disorder is a form of plasma cell dyscrasia in which no myeloma protein is detected in serum or urine (at least as determined by conventional laboratory methods) of individuals who have clear evidence of an increase in clonal bone marrow plasma cells and/or evidence of clonal plasma cell-mediated tissue injury (e.g. plasmacytoma tumors). (wikipedia.org)
  • gene
  • Customers obtain a clonal HAP1 cell line, bearing a frameshift mutation in an exon of the gene of interest. (horizondiscovery.com)
  • However, the tax gene transcript could not be detected in fresh ATL cells regardless of hyperacetylated histone H3 in 5'-LTR. (biomedcentral.com)
  • These results showed that epigenetic changes of provirus facilitated ATL cells to evade host immune system by suppressing viral gene transcription. (biomedcentral.com)
  • This discovery changed the current thinking about cancer from a model wherein cancer is caused by a foreign substance (a viral gene) to one where a gene that is normally present in the cell can cause cancer. (wikipedia.org)
  • The protein encoded by this gene is a nucleolar antigen expressed in proliferating cells. (wikipedia.org)
  • Hematopoietic progenitor cell antigen CD34 also known as CD34 antigen is a protein that in humans is encoded by the CD34 gene. (wikipedia.org)
  • Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. (wikipedia.org)
  • Mutations of the GLI2 gene are associated with several phenotypes including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. In human keratinocytes Gli2 activation upregulates a number of genes involved in cell cycle progression including E2F1, CCND1, CDC2 and CDC45L. (wikipedia.org)
  • As they are stimulated to become plasma cells, B cells refashion parts of their genome in efforts to create a new gene that encodes a functional antibody. (wikipedia.org)
  • If these gene alterations are successful in coding for a functional antibody (termed Ig for immunoglobulin), the maturing B cells and to a greater extent their plasma cell descendants make and secrete an intact antibody, initially IgM but after class switch recombinations, either IgG, IgA, IgE, or IgD. (wikipedia.org)
  • However, the cited gene changes can go awry in plasma cells by, for example, placing a gene that ordinarily controls cell growth adjacent to the normally highly active antibody gene promotor thereby creating a cancer-causing oncogene or, more commonly, by forming extra chromosomes (see trisomy) or chromosomes that have deleted or repetitive sections, any of which such changes may promote malignancy in more complex and less well understood ways. (wikipedia.org)
  • Components
  • Components and strategies Cell lifestyle MCF-7 cells had been grown up in DMEM moderate filled with 10% fetal leg serum and 1% penicillin/streptomycin serum as defined (9). (cancercurehere.com)
  • The role of telomeres and telomerase in cell aging and cancer was established by scientists at biotechnology company Geron with the cloning of the RNA and catalytic components of human telomerase and the development of a polymerase chain reaction (PCR) based assay for telomerase activity called the TRAP assay, which surveys telomerase activity in multiple types of cancer. (wikipedia.org)
  • Human
  • We conclude that millicurrent stimulation of human osteoarthritic chondrocytes cultivated in a 3 D collagen gel and of osteochondral explants directly influences cell metabolism. (biomedcentral.com)
  • McCauley LK , Rosol TJ, Merryman JI, Capen CC. Parathyroid hormone-related protein binding to human T cell lymphotropic virus type I-infected lymphocytes. (dentisty.org)
  • Effects of hormones and cytokines on stimulation of adenylate cyclase and intracellular calcium concentration in human and canine periodontal ligament cells. (dentisty.org)
  • Dexamethasone enhances the effects of parathyroid hormone (PTH) on human periodontal ligament (PDL) cells in vitro . (dentisty.org)
  • Human T-cell leukemia virus type I (HTLV-I) causes adult T-cell leukemia (ATL) after a long latent period. (biomedcentral.com)
  • CFL1 Full-Length MS Protein Standard (NP_005498), Labeled with [U- 13C6, 15NL-Arginine and [U- 13C6, 15NL-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. (creative-proteomics.com)
  • The retina is protected by the blood-retinal barrier (BRB) which is composed of human retinal microvascular endothelial cells (HRMECs) and retinal pigment epithelium (RPE). (hindawi.com)
  • The team of researchers performed tests on a variety of cells that carried the tat protein and observed the rate of cell proliferation in cells infected by HIV-1 and the control, a culture of healthy human endothelial cells. (wikipedia.org)
  • invasion
  • of TRPC decreased the cell routine S stage and cell migration, implicating an operating function for TRP-mediated Ca2+ entrance in cell proliferation and invasion. (cancercurehere.com)
  • Additionally, our outcomes also claim that TRPC3 shows up as a fresh mediator of breasts cancer tumor cell migration/invasion and represents a potential focus on for a fresh course of anticancer agent. (cancercurehere.com)
  • Ca2+ entrance via turned on TRPC was improved when PUFA had been absent, recommending a double-gating system for Danshensu TRPC which may be involved with MCF breasts cancer tumor cell proliferation and invasion. (cancercurehere.com)
  • processes
  • It has been associated with heterochromatin and PML-NBs (Promyelocytic Leukaemia nuclear bodies) and has been implicated in many nuclear processes including transcription and cell cycle regulation. (wikipedia.org)
  • vitro
  • Osteochondral explants and cell-seeded gel samples were cultivated in-vitro for three weeks. (biomedcentral.com)
  • McCauley LK , Rosol TJ, Capen CC. Effects of cyclosporin A on rat osteoblasts (ROS 17/2.8 cells) in vitro . (dentisty.org)
  • Merryman JI, Rosol TJ, McCauley LK , Werkmeister JR, Suter MM , Capen CC. Regulation of parathyroid hormone-related protein production by a squamous carcinoma cell line in vitro . (dentisty.org)
  • Secretion of parathyroid hormone-related protein by bovine mammary cells in vitro . (dentisty.org)
  • The transcription rapidly recovered after in vitro culture in such ATL cells. (biomedcentral.com)
  • Bone
  • Methods and Results- Immediately after hindlimb ischemia was created, rats were randomized to receive AM infusion plus bone marrow-derived mononuclear cell (MNC) transplantation (AM+MNC group), AM infusion alone (AM group), MNC transplantation alone (MNC group), or vehicle infusion (control group). (ahajournals.org)
  • fluorescence
  • Adjustments in the Ca2+ focus had been indicated as F/F, where F was the fluorescence strength when cells had been at rest, and F was the modification in fluorescence during excitement. (cancercurehere.com)
  • subunits
  • C-jun has been shown to be essential for fibroblast proliferation, and levels of both AP-1 subunits have been shown to be expressed above basal levels during cell division. (wikipedia.org)
  • transfection
  • MCF-7 cells had Danshensu been passaged onto coverslips in 500 l Opti-MEM (Invitrogen) 1 day before transfection and reached about 40C50% confluence during transfection. (cancercurehere.com)
  • serum
  • The heterodimer also stimulated cAMP production and thymidine incorporation by cultured thyroid cells and increased serum thyroxine levels in TSH-suppressed rats in vivo. (jci.org)
  • HAP1 cells are grown in Iscove's Modified Dulbecco's Medium (IMDM) in the presence of 10% fetal calf serum and penicillin/ streptomycin. (horizondiscovery.com)
  • malignant
  • The overproduced Ig's, termed myeloma proteins, commonly circulate in blood, may accumulate in urine, and are the hallmarks of plasma cell dyscrasias including their most malignant forms viz. (wikipedia.org)
  • basal
  • Transgenic mice over-expressing the transcription factor Gli2 under the K5 promoter in cutaneous keratinocytes develop multiple skin tumours on the ears, tail, trunk and dorsal aspect of the paw, resembling those of basal cell carcinoma (BCC). (wikipedia.org)
  • oncogenes
  • Cells can also be induced to senesce via DNA damage in response to elevated reactive oxygen species (ROS), activation of oncogenes and cell-cell fusion, independent of telomere length. (wikipedia.org)
  • Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. (wikipedia.org)
  • metabolism
  • Like the nine other distinct FABPs that have been identified, H-FABP is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. (wikipedia.org)
  • types
  • Src, Fyn and Yes are expressed ubiquitously in all cell types while the others are generally found in hematopoietic cells. (wikipedia.org)
  • However, loss of Gli3 leads to abnormal patterning and loss of Gli2 affects the development of ventral cell types, most significantly in the floor plate. (wikipedia.org)
  • reagent
  • Cell routine evaluation After removal of methanol, cells had been treated having a Coulter DNA-Prep reagent package (Beckman-Coulter, France). (cancercurehere.com)