• Thus, agents that inhibit cell proliferation and restrain hepatic tumorigenesis through cell cycle regulation have a beneficial effect in the treatment of hepatocellular carcinogenesis. (greenmedinfo.com)
  • These sex hormones also inhibit cell proliferation once the target organs achieve full development (proliferative shutoff effect). (tufts.edu)
  • The demonstration by Upham et al 1 that human alveolar macrophages selectively inhibit proliferation of T cells by secretion of unidentified effector molecules raises the question as to whether pathological processes in the lung characterised by extensive macrophage recruitment or activation can have a systemic effect on T cell development. (bmj.com)
  • Incubation of RPCs with either high glucose or MGO reduced the activity of RPCs to inhibit activated T cell proliferation. (arvojournals.org)
  • Al‐Nasiry S, Geusens N, Hanssens M, Luyten C and Pijnefnborg R (2006) The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells. (els.net)
  • Restraint stress modifies Langerhans cell migration and lymph node cell proliferation. (cdc.gov)
  • LC morphology and migration were examined in epidermal sheets by counting the number of FITC conjugated lIa stained cells/mm2 at 0, 2 & 18 hours after chemical application. (cdc.gov)
  • These data suggest that restraint-induced decrease in T cell proliferation may be due, in part, to decreased LC migration to the local lymph nodes. (cdc.gov)
  • It is believed that it regulates the many downstream genes which produce the pathology of restenosis, namely cell migration and adhesion, collagen formation, secretion of extra-cellular matrix, and cell proliferation , among others. (thefreedictionary.com)
  • The present study aimed to analyze the effects of indirubin on cell proliferation, migration, invasion, and angiogenesis of tumor-derived endothelial cells (Td-EC). (dovepress.com)
  • Myeloma cell lines and primary samples were tested for the combined effects of IL-6 and HGF in inducing DNA synthesis and migration. (wiley.com)
  • The results indicate that besides from being a myeloma growth factor alone, HGF can also potentiate the effects of IL-6 in myeloma proliferation and migration. (wiley.com)
  • This text also deals with topics such as the use of cloned SV40 DNA fragments to examine signals for cell proliferation, expression of dihydrofolate reductase and thymidylate synthase genes in mammalian cells, and gene expression during the cell cycle of Chlamydomonas reinhardtii. (elsevier.com)
  • 9 LincRNAs are transcribed abundantly in mammalian cells and generally show developmental stage-, tissue- or disease-specific expression patterns. (nature.com)
  • Although the mechanisms and proteins of mitochondrial fusion are well known in yeast and mammalian cells, they remain poorly understood in plant cells. (biomedsearch.com)
  • The G1 checkpoint, also known as the restriction point in mammalian cells and the start point in yeast, is the point at which the cell becomes committed to entering the cell cycle. (wikipedia.org)
  • Many organisms have ≈24-h rhythms in metabolism, physiology, and behavior that are driven by cell autonomous circadian pacemakers ( 1 ). (pnas.org)
  • Alternatively, cell proliferation can also be analyzed with cell viability assays that measure the rate of cellular metabolism, such as MTT, MTS, resazurin and similar assays, mitochondrial membrane potential dependent dyes, cellular esterase cleaved dyes, ATP and ADP assays, and assays that measure glycolytic flux and oxygen consumption. (abcam.com)
  • Contrary to the common assumption that proliferating cells increase glycolysis at the expense of mitochondrial metabolism, their results show that glycolysis and mitochondrial respiration increase by equal amounts during proliferation. (isotope.com)
  • Rapidly proliferating cells reshape their metabolism to satisfy their ever-lasting need for cellular building blocks. (mcponline.org)
  • How cells reshape their metabolism is not fully understood. (mcponline.org)
  • Here we report that loss of cathepsin L (Cts L) is associated with a fast proliferation rate and enhanced glycolytic metabolism that depend on lactate dehydrogenase A (LDHA) activity. (mcponline.org)
  • Using mass spectrometry analysis of cells treated with a pan cathepsin inhibitor, we observed an increased abundance of proteins involved in central carbon metabolism. (mcponline.org)
  • Cells have the fundamental ability for metabolism remodeling in response to changing environments. (mcponline.org)
  • The latter has recently sparked continued interest since the rejuvenation of the lysosomes as a central hub for cell metabolism ( 4 ). (mcponline.org)
  • The lysosomes receive nutritional cues from various sources such as the extracellular environment or intracellular pools and dynamically regulate cell metabolism ( 5 ). (mcponline.org)
  • But changes in cells can also occur whenneurotransmitters induce a general state of inflammation or alter blood flow, an indirect route of action for the ANS. (news-medical.net)
  • While isolated bouts of inflammation did not induce HR, overlap between inflammation-induced DNA damage and inflammation-induced cell proliferation induced HR significantly. (nih.gov)
  • We found that exposure to alkylation damage induces HR, and importantly, that inflammation-induced cell proliferation and alkylation induce HR in a synergistic fashion. (nih.gov)
  • hence, sex hormones would induce cell proliferation by neutralizing the effect of the plasma-borne inhibitors. (tufts.edu)
  • Estradiol (E2) was demonstrated to induce endometrial cell proliferation, whereas medroxyprogesterone (MPA) inhibited endometrial cell proliferation via antagonizing estrogenic effects. (clinicaltrials.gov)
  • Utilizes the most widely accepted detection reagents, tetrazolium salts, for the safe, accurate, and straightforward quantification of changes in cell proliferation. (atcc.org)
  • M2 macrophages isolated from the pancreas of mice subjected to PDL showed greater expression of TGFβ1 , which encodes a ligand for TGFβ receptors, and chemical inhibition of type I TGFβ receptors inhibited proliferation of β cells cocultured with M2 macrophages. (sciencemag.org)
  • In asmuch as they express prolactin receptors, respond to prolactin treatment, and synthesize their own prolactin ( 19 - 22 ), these cells are similar to B and T lymphocytes, whose proliferation has been shown to be stimulated by the hormone ( 23 , 24 ) and inhibited by cannabinoids, anandamide ( 10 ), and 2-arachidonoylglycerol ( 11 ). (pnas.org)
  • When neurotransmitters bind to receptors in the membranes of certain cells, they elicit a direct response within the cell. (news-medical.net)
  • They found not only that the stem cells did have receptors for ANS neurotransmitters, but also the neurotransmitters changed the behavior of the cells - just what they would expect to see for a direct relationship. (news-medical.net)
  • When we isolated the stem cells and found there were actually ANS neurotransmitter receptors, we found that missing piece,' Davis says. (news-medical.net)
  • T-cell clonotypes from different UA patients used antigen receptors with similar sequences. (ahajournals.org)
  • Our previous results revealed that the expression of killer inhibitory receptors on natural killer cells was decreased in eutopic endometrium in women with adenomyosis. (clinicaltrials.gov)
  • Endothelin-1 (ET-1) is a powerful vasoconstricting peptide produced predominantly by vascular endothelial cells, that exerts its effect through the interaction with specific receptors, ETA. (bioportfolio.com)
  • Recently, the angiotensin II receptors AT1 and AT2 were found to be expressed on rat neuronal stem cells. (nova.edu)
  • We analyze the effect of TGF- β 1 inLeydig cells depending on the type of receptors involved in the signaling pathway of TGF- β 1. (scirp.org)
  • On the basis of our studies and from those of other authors we conclude that the balance between the expression of TGF- β 1 receptors and co receptors is of relevance in Leydig cell physiology and pathophisiology. (scirp.org)
  • Cell proliferation assays are mainly designed based on three concepts, that is, (1) measuring rate of DNA replication, (2) analysis of metabolic activity and (3) cell surface antigen recognitions. (els.net)
  • Cell proliferation antigen‐based assay targets antigens present in proliferating cells such as markers like Ki‐67, topoisomerase IIB, phosphohistone H3 and PCNA. (els.net)
  • Scheme of antigen‐based cell proliferation assay. (els.net)
  • We hypothesize that these dichotomous effects of restraint would be reflected in changes in LC trafficking from the epidermis or alteration of their antigen presenting capabilities to T cells in the draining lymph nodes. (cdc.gov)
  • Multiple interactions also occur between activated immune cells that are critical for regulation of the immune response (for instance, T cell interactions among themselves and also with antigen-presenting cells). (sartorius.com)
  • Shared T-cell receptor sequences in clonotypes of different patients implicate chronic stimulation by a common antigen, for example, persistent infection. (ahajournals.org)
  • During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
  • Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
  • We show that inflammation induces DSBs (γH2AX foci) and that several days later there is an increase in cell proliferation. (nih.gov)
  • Taken together, these results show that, during an acute bout of inflammation, there is a kinetic barrier separating DNA damage from cell proliferation that protects against mutations, and that inflammation-induced cell proliferation greatly potentiates exposure-induced mutations. (nih.gov)
  • Compared with traditional methods, the team's novel matrix can reduce the risk of carcinogenesis or inflammation in stem cell therapy - a treatment that offers hope of a cure for incurable diseases such as neurodegenerative diseases, chronic systemic diseases and degenerative joint diseases. (news-medical.net)
  • Professor Ken Yung said, 'The novel matrix enables scientists to cultivate NSCs by adopting the usual method, however with the added advantage of organic compounds (like Polylysine and Polyornithine) being excluded from the process, thereby reducing the potential risk of carcinogenesis or inflammation in stem cell therapy. (news-medical.net)
  • The initial trapping of T cells in transit is due, at least in part, to local endothelial expression of inflammation associated molecules such as ICAM-1. (bmj.com)
  • An overview of the biochemical aspects of cell proliferation and the genes and gene products that are necessary and specific for cell proliferation concludes the book. (elsevier.com)
  • In addition, they express higher levels of CXCL13 and CCL19 chemokines, as well as MAdCAM-1 and VCAM-1 cell-adhesion molecules. (jimmunol.org)
  • TIP47 protects mitochondrial membrane integrity and inhibits oxidative-stress-induced cell death. (biomedsearch.com)
  • We found that overexpression of tail interacting protein of 47 kDa (TIP47), but not its truncated form (t-TIP47) protected NIH3T3 cells from hydrogen-peroxide-induced cell death, prevented the hydrogen-peroxide-induced mitochondrial depolarization determined by 5,50,6,60-tetrachloro-1,10,3,30-tetraethyl-benzimidazolylcarbocyanine iodide (JC1), while suppression of TIP47 in HeLa cells facilitated oxidative-stress-induced cell death. (biomedsearch.com)
  • Here, we quantified the copy numbers of mitochondrial DNA (mtDNA) in the gametic cells of angiosperm species. (biomedsearch.com)
  • To divide, cells must duplicate a copy of their DNA, increase mitochondrial density, and assemble/synthesize microtubules during interphase. (biolegend.com)
  • The colorimetric assay is based on the cleavage of the tetrazolium salt WST-1 to a formazan-class dye by mitochondrial succinate-tetrazolium reductase in viable cells. (clontech.com)
  • Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. (biovision.com)
  • Conversely, mice with β cell-specific Notch gain of function (β-NICD) had a progressive loss of β cell maturity, due to proteasomal degradation of MafA, leading to impaired glucose-stimulated insulin secretion and glucose intolerance with aging or obesity. (jci.org)
  • We demonstrate here that (a) TGF-c ~ production and secretion by mammary cells is downregulated by the basement membrane-dependent alveolar structure, and (b) compared with/3-casein, WAP expression is preferentially inhibited both in culture and in transgenic mice when TGF-ot is added or overexpressed. (psu.edu)
  • Foremost, it inhibits gastric secretion and enhances insulin release by pancreatic β islet cells in the presence of glucose (for review, see Yip and Wolfe, 2000 ). (jneurosci.org)
  • Preclinical and clinical data to date suggests superior safety and tolerability, as well as increased potency versus Methotrexate (MTX), currently the leading antifolate treatment and standard of care for a broad range of abnormal cell proliferation diseases. (thefreedictionary.com)
  • Abnormal cell proliferation has been generally found in the tumorigenesis, including the formation of endometriosis. (clinicaltrials.gov)
  • Adenomyosis is considered to have a similar pathophysiology with endometriosis, and it must be interesting to examine whether there is abnormal cell proliferation in the eutopic endometrium of adenomyosis. (clinicaltrials.gov)
  • Therefore, whether abnormal cell proliferation occurs under the effects of LPS and IFN-g in the eutopic endometrium of adenomyosis needs further clarification. (clinicaltrials.gov)
  • Induction of beta-cell proliferation by either 5-IT or harmine, another natural-product DYRK1A inhibitor, was suppressed by co-incubation with the calcineurin inhibitor FK506, suggesting involvement of DYRK1A and NFAT signaling. (diabetesjournals.org)
  • These results point to inhibition of DYRK1A as a therapeutic strategy to increase human beta-cell proliferation. (diabetesjournals.org)
  • Prior to Davis's study, which is published in Physiological Reports , scientists had suspected the ANS was involved in stem cell proliferation, but they didn't know if the relationship was direct or indirect. (news-medical.net)
  • To demonstrate that stem cell behavior was changing as a result of ANS stimulation, the researchers grew intestinal epithelial cells in the lab and exposed them to high levels of two neurotransmitters, norepinephrine and acetylcholine. (news-medical.net)
  • When we simulated activation of either of those systems, we saw a decrease in stem cell proliferation,' Dailey says. (news-medical.net)
  • Although the research focused on the intestinal epithelium, Davis and Dailey suspect the ANS is directly controlling stem cell proliferation in other parts of the body, as well. (news-medical.net)
  • Derivation and characterization of haploid embryonic stem cell cultures in medaka fish. (biomedsearch.com)
  • Megan Dailey (left) and Elizabeth Davis (right), both from the University of Illinois' animal sciences department, have discovered that the autonomic nervous system exerts direct control over stem cell proliferation. (eurekalert.org)
  • Recently, scientists have turned their focus on cell replacement therapies, including stem cell therapy, which have shown huge promise in treating neurodegenerative diseases. (news-medical.net)
  • Stem cell therapy is a treatment using stem cells to cultivate new and normal cells, tissues or organs and then transplanted to people to restore physiological function by replacing damaged or dead cells. (news-medical.net)
  • After growth and cell differentiation, it is hoped that the mature cell can turn into a therapeutic agent for stem cell therapy. (news-medical.net)
  • It could provide a safe platform for research into stem cell therapies using the latest, novel nanotechnology, and also help boost the development of regenerative medicine. (news-medical.net)
  • Alterations in neural stem cell niches have been associated with spatial learning and memory and cognition. (nova.edu)
  • These findings may demonstrate a role for theangiotensin system as a novel target to regulate neural stem cell function with implications for enhancing age-related detriments in learning and memory, regeneration following injury, and therapeutic intervention across a broad spectrum of neurodegenerative diseases. (nova.edu)
  • In the present study, the effects of tehranolide on CaM, PDE1, and the cAMP/PKA signaling pathway to define the mechanism of cell growth inhibition were investigated. (springer.com)
  • Notch signaling regulates differentiation of the pancreatic endocrine lineage during embryogenesis, but the role of Notch in mature β cells is unclear. (jci.org)
  • These findings suggest that HIF2A is a pivotal mediator of hypoxia signaling in satellite cells and may be therapeutically targeted to improve muscle regeneration. (jci.org)
  • In addition, the inhibitor of PI3K/Akt signaling pathway, LY294002, dramatically inhibited the growth of Ubc9 overexpressing cells. (mdpi.com)
  • IL-6-treatment increased the expression of c-Met making cells HGF responsive, and IL-6 was dependent on c-Met signaling in activating both Ras and p44/42 MAPK by a mechanism involving the tyrosine phosphatase Shp2. (wiley.com)
  • They also discovered that ONA inhibited pro-tumor activities of myeloid-derived suppressor cells (MDSC), which the researchers say are linked with the suppression of the anti-tumor immune response of host lymphocytes. (medicalnewstoday.com)
  • Ahmed SA, Gogal RM and Walsh JE (1994) A new rapid and simple nonradioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assays. (els.net)
  • The unstable plaque but not the stable plaque is invaded by clonally expanded T cells, suggesting a direct involvement of these lymphocytes in plaque disruption. (ahajournals.org)
  • A key to developing effective immunotherapeutics is an understanding of the mechanisms of immune cell activation, proliferation, and regulation. (sartorius.com)
  • 3 8 9 Stimulation of inflammatory mechanisms in ACS is not, however, limited to the microenvironment of the plaque, because it also involves circulating cell populations. (ahajournals.org)
  • 17 An understanding of the pathophysiology of CD4 + CD28 null T cells and the mechanisms leading to their expansion may provide insights into the cause of ACS and into the roles played by T cells and macrophages in plaque rupture. (ahajournals.org)
  • 1-1 1-3 1-5 While the precise mechanisms employed by the macrophages to modulate T cells are incompletely understood, it is clear that their overall efficiency in this regard is a reflection of their maturation/activation status. (bmj.com)
  • To investigate the effect of Luteolin on proliferation and cell death of human multiple myeloma cell line RPMI-8226 and its mechanisms. (greenmedinfo.com)
  • Cell cycle checkpoints are control mechanisms in eukaryotic cells which ensure proper division of the cell. (wikipedia.org)
  • When DNA damage occurs, or when the cell detects any defects which necessitate it to delay or halt the cell cycle in G1, arrest occurs through several mechanisms. (wikipedia.org)