• Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
  • To evaluate the effect of Exendine-4 (EX-4), a Glucagon-like peptide 1 (GLP-1) receptor agonist, on the differentiation of insulin-secreting cells (IPCs) from rat adipose-derived mesenchymal stem cells(ADMSCs). (celljournal.org)
  • The aim of this study was to differentiate canine adipose-derived mesenchymal stem cells (ADMSCs) into insulin-producing cells by using culture media with different compositions to determine the most efficient media. (sdlifesciences.com)
  • Adipose-derived mesenchymal stem cells from groups 1 to 5 gradually became round in shape and gathered in clusters. (sdlifesciences.com)
  • Insulin-producing cells (IPCs) differentiated from mesenchymal stem cells (MSCs) hold great promise in the treatment of DM. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) have gained interest because of its potential application in regeneration medicine and cytotherapy. (biomedcentral.com)
  • Mesenchymal Stem Cells (MSCs) can be isolated from several body tissues, including dental tissues. (bvsalud.org)
  • The ability to restore cells and tissue function without the need of immunosuppressive drugs and without the concern for tissue compatibility makes Mesenchymal Stem Cells (MSCs, usual acronym) a strong promise for the future. (bvsalud.org)
  • Researchers at the Center for iPS Cell Research and Application at Kyoto University in Japan presented data at a meeting of the American Society of Hematology showing they were able to create the cells in the laboratory and confirm they had the same life span as normal human platelets when infused in mice. (ibtimes.com)
  • The Japanese researchers set out to create an immortalized cell line with a large number of high-quality megakaryocytes -- precursor cells that develop into platelets -- from stem cells that can be grown indefinitely and differentiate into a variety of cell types in the body. (ibtimes.com)
  • Researchers previously had been able to produce neurons - the impulse-conducting cells in the brain and spinal cord - from human embryonic stem cells. (sciencedaily.com)
  • These features allowed the researchers to exploit an endogenous DNA repair system in the cell called microhomology-mediated end joining (MMEJ) in order to precisely remove the reporter gene. (medindia.net)
  • Researchers identified that an abundance of fungi in the gut, particularly strains of Candida albicans yeast, could trigger an increase in immune cells, which could worsen lung damage. (medicaldaily.com)
  • Friday, U.S. and British researchers reported that diabetes could be treated by using stem cells taken from the umbilical cords of newborns. (cellmedicine.com)
  • Researchers were able to take stem cells, expand them into a large number, and direct them to be similar to the insulin producing cells. (cellmedicine.com)
  • Researchers have created sterile animals capable of producing the fertile sperm of a different species through a technique called blastocyst complementation. (iflscience.com)
  • Stem cell research harnesses the versatility of cell types to produce tissue and organs but creating gametes such as sperm and egg have proven difficult to achieve (though researchers did recently make a mouse embryo from stem cells ). (iflscience.com)
  • The researchers, based at Tsinghua University, Beijing, China, and Drexel University, Philadelphia, USA, used extrusion-based 3-D printing to produce a grid-like 3-D structure to grow embryoid body that demonstrated cell viability and rapid self-renewal for 7 days while maintaining high pluripotentcy. (phys.org)
  • The researchers hope that this technique can be developed to produce embryoid body at a high-throughput, providing the basic building blocks for other researchers to perform experiments on tissue regeneration and/or for drug screening studies. (phys.org)
  • To address the problem, the researchers took a stepwise approach to create beta cells. (sciencedaily.com)
  • The researchers will continue to study this technique in the lab to further optimize the production of beta cells. (sciencedaily.com)
  • Researchers have constructed a laboratory model for a unique neurological disorder by transforming patients' own cells using stem cell technology. (lifeboat.com)
  • In this experiment, the researchers grew a human embryonic stem cell line and 2 human-induced pluripotent stem cell lines in a culture system that allowed them to produce large numbers of cells. (pharmaceuticalintelligence.com)
  • The researchers tested more than 150 combinations of over 70 compounds to figure out a method to produce functional human beta cells from the cultured stem cells which when added in exact combinations over a period of several weeks, they transformed human pluripotent stem cells into beta cells that functioned similarly to normal adult beta cells. (pharmaceuticalintelligence.com)
  • A distinctive feature of this report is an end-user survey of 273 researchers (131 U.S. / 143 International) that identify as having induced pluripotent stem cells as a research focus. (prnewswire.com)
  • Pluripotent stem cells are an attractive option for researchers from a therapeutic standpoint because they can self-renew in lab cultures and can differentiate into a variety of cell types. (medicalnewstoday.com)
  • Researchers have previously used hPSCs to create insulin-producing beta cells. (medicalnewstoday.com)
  • Targeting this structure allows the researchers to create fewer irrelevant cells and better functioning beta cells that helped control blood sugar. (medicalnewstoday.com)
  • It did so by promoting the proliferation of brain stem cells and their development into neutrons, the researchers noted. (express.co.uk)
  • At a median follow-up of 4 years, the researchers have seen no safety signals related to transplantation of RPE cells. (medscape.com)
  • Pretreatment with a stem-cell-activating protein significantly enhances healing in mice, Stanford researchers say. (stanford.edu)
  • These cells entered what the researchers called an "alert" phase of the cell cycle that is distinct from either fully resting or fully active stem cells. (stanford.edu)
  • After 2.5 days, the researchers found that muscle stem cells from the recipient animals were in an alert state and completed their first cell division much more quickly than occurred in animals that had received blood serum from uninjured mice. (stanford.edu)
  • Researchers at Georgetown University Medical Center have taken tissue from human testicles that produce sperm, grafted them onto diabetic mice and showed that blood sugar levels can be controlled for up to a week because they produce insulin. (sciencemediacentre.org)
  • Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)
  • As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
  • Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)
  • By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
  • But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
  • Because cancer cells divide faster than normal cells, they are more affected by chemotherapy compared to healthy cells", explains Helena Ã…gerstam, cancer researchers at Lund University. (lu.se)
  • Dr. Takehiko Kaneko, MD is CMO and head of R&D of Heartseed Inc. Before joining Heartseed, he served on the leadership team at SanBio Co. Ltd., where he was responsible for the development and regulatory standards of cell therapy products. (fiercebiotech.com)
  • The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
  • While the individual unit operations for making a cell therapy product are always the same, the tools and technologies utilized in those unit operations are still evolving and adapting to industry needs. (genengnews.com)
  • To try and address this, Cellares developed what it claims is the first industrial-scale cell therapy manufacturing solution. (genengnews.com)
  • The technology, called Cell Shuttle, is described as a cell therapy production factory-in-a-box that uses automation in a closed system. (genengnews.com)
  • In contrast to any other cell therapy manufacturing solution on the market today, it has the ability to produce up to 10 patient doses simultaneously," Gerlinghaus continues. (genengnews.com)
  • Tools like AI and machine learning will have a positive impact on cell therapy manufacturing. (genengnews.com)
  • In fact, we are incorporating these technologies into the Cell Shuttle's software to mine and analyze the vast amounts of in-process data using machine learning algorithms to help improve the cell therapy manufacturing processes moving forward. (genengnews.com)
  • The cell therapy sector is in dire need of better, more efficient higher capacity manufacturing technologies, Gerlinghaus says. (genengnews.com)
  • Today the pioneering cell therapy companies struggle to treat just over 1,000 patients per year. (genengnews.com)
  • The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
  • Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
  • Research published in the journal of Stem Cell Research and Therapy in 2014, found that ar-turmerone contributed to the development of new neurons. (express.co.uk)
  • But this is just a first step in the long road towards making regenerative cell therapy a reality in macular and retinal degeneration. (medscape.com)
  • Patients who have had stem cell or exosome therapy should discuss any concerns and abnormal reactions with their healthcare provider. (cdc.gov)
  • An investigational stem cell-based therapy for the treatment of Parkinson's Disease, STEM-PD, has been given regulatory approval for a Phase I/IIa clinical trial. (lu.se)
  • WCMM members Agnete Kirkeby and Gesine Paul-Visse are part of the team behind STEM-PD - a new kind of dopaminergic nerve cell therapy designed to replace cells lost in Parkinson's disease, the first of its kind in Europe! (lu.se)
  • As a result of being capable of differentiating into a variety of cell types, it can be presumed that stem cell therapy has an advantage when compared to other tissue repair methods. (bvsalud.org)
  • The high regeneration potential has aroused a great interest in the scientific community 3 , due to its many clinical applications in cell therapy or tissue engineering. (bvsalud.org)
  • Cell therapy is a therapy where cellular material is injected systemically or directly into the injured tissue, to promote local repair or to restore systemic health. (bvsalud.org)
  • Reuters) - Scientists have for the first time created blood platelet cells by reprogramming stem cells derived from adult cells, offering the potential for a renewable supply of the fragile blood component. (ibtimes.com)
  • They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
  • The scientists of the BioRescue consortium have already produced northern white rhino embryos by in vitro fertilisation of oocytes with sperm. (izw-berlin.de)
  • citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
  • Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
  • In 2007, when scientists first reported they had reprogrammed skin cells into stem cells, it was hailed as an alternative to getting stem cells from embryos , which are then destroyed. (foxnews.com)
  • Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)
  • Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)
  • Stem cells are classified into two main: embryonic stem cells, which are found in the embryos and adult stem cells, found in adult tissues. (bvsalud.org)
  • These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
  • The problem, Gerlinghaus points out, is that producing autologous cell therapies is laborious, failure-prone, and extremely difficult to scale. (genengnews.com)
  • What's the difference between allogeneic and autologous stem cell transplantations? (clevelandclinic.org)
  • This article aims to understand how adherence to biotechnology of stem cell storage of umbilical cord for autologous use produces the adoption of practices, in the present, that aim to prevent risks in the name of biological security in the future. (bvsalud.org)
  • If you're studying a disease found in a certain part of the brain, you should use a human embryonic stem cell line that produces the neurons from that region of the brain to get the most accurate results from your study. (sciencedaily.com)
  • Sun said there are ways to prod an embryonic stem cell line biased to become neurons found in the rear brain to become neurons found in the forebrain. (sciencedaily.com)
  • Novavax to Use Vivalis' Duck Embryonic Stem Cell Line for Vaccine. (genengnews.com)
  • 4) Generated a motor neuron reporter human embryonic stem cell line. (ca.gov)
  • Induced pluripotent stem cells, or iPS cells, are produced by manipulating ordinary human skin or blood cells back to a state in which they are able to differentiate into a number of different cell types. (ibtimes.com)
  • Sun's method prodded human embryonic stem cells to differentiate into neural stem cells, the cells that give rise to neurons. (sciencedaily.com)
  • Using the same techniques to prod the two embryonic stem cells lines to differentiate, Sun found that one line had a bias to become neurons that are found in the forebrain. (sciencedaily.com)
  • These cells can grow indefinitely in the laboratory and can differentiate, into any cell type found in the body. (pharmaceuticalintelligence.com)
  • In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
  • While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)
  • Neural stem cells (NSCs), found in the brain and spinal cord, are special cells that can differentiate into different cell types in the central nervous system, such as neurons. (express.co.uk)
  • In the other location-called the hair germ area-McSCs can differentiate to form melanocytes that produce melanin pigment for hair. (medicalnewstoday.com)
  • In other words, during each growth cycle, the McSCs can differentiate into a pigment-producing partially differentiated state and then revert back to an undifferentiated state. (medicalnewstoday.com)
  • Stem cells in the body are responsible for the regeneration of tissue in the body and can differentiate to form an array of specialized cells. (medicalnewstoday.com)
  • Specifically, the division of a stem cell can result in the formation of identical daughter stem cells and/or cells that can differentiate to assume different fates. (medicalnewstoday.com)
  • While some patients in her study have gained vision, Ninel Z. Gregori, MD, from Bascom Palmer Eye Institute, Miami, Florida, said, "These trials show long term safety and possible biologic activity of pluripotent stem-cell derived progeny, but they were not powered to show efficacy. (medscape.com)
  • The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
  • It is also clear that pro-mitotic factors such as C-MYC/L-MYC or repression of cell cycle checkpoints, such as p53, are conduits to creating a compliant cellular state for iPSC reprogramming. (wikipedia.org)
  • Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)
  • Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)
  • Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
  • The transplant procedure requires the harvesting of hematopoietic stem cells from a donor. (medscape.com)
  • Hematopoietic stem cells (HSCs) are produced by a small cohort of hemogenic endothelial cells (ECs) during development through the formation of intra-aortic hematopoietic cell (HC) clusters (HCs). (researchdataservice.com)
  • Aortic hematopoiesis is normally characterized by the creation of little groupings of hematopoietic cells (HCs) that accumulate in the lumen, carefully linked with the endothelial flooring (Dieterlen-Livre et al. (researchdataservice.com)
  • 2011). Live image resolution methods demonstrated that embryonic control cells produced ECs which, in convert, created hematopoietic cells (Eilken et al. (researchdataservice.com)
  • 2002) and Golvatinib appears to end up being needed for the first stages of hematopoietic cell development from the endothelium, but dispensable for the afterwards types (Chen et al. (researchdataservice.com)
  • Taking into consideration that aortic hematopoiesis originates from hemogenic ECs, it can end up being seen as a cell destiny transformation in which ECs loose their features and acquire hematopoietic-specific indicators (Jaffredo et al. (researchdataservice.com)
  • Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
  • We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. (nih.gov)
  • Stage (S) 7 cells expressed key markers of mature pancreatic beta cells, including MAFA, and displayed glucose-stimulated insulin secretion similar to that of human islets during static incubations in vitro. (nih.gov)
  • Additional characterization using single-cell imaging and dynamic glucose stimulation assays revealed similarities but also notable differences between S7 insulin-secreting cells and primary human beta cells. (nih.gov)
  • Therefore, although S7 cells are not fully equivalent to mature beta cells, their capacity for glucose-responsive insulin secretion and rapid reversal of diabetes in vivo makes them a promising alternative to pancreatic progenitor cells or cadaveric islets for the treatment of diabetes. (nih.gov)
  • The cells could be engineered to produce insulin. (cellmedicine.com)
  • Insulin-producing cells in the pancreas are damaged by diabetes. (cellmedicine.com)
  • They were used for stimulation of mesenchymal stem cell differentiation into insulin-producing cells. (ualr.edu)
  • Based on our in vitro studies, we have concluded that both the POMs tested had significant biological activity acting as active stimuli for differentiation of stem cells into insulin-producing cells. (ualr.edu)
  • Scientists are developing a promising approach for treating type 1 diabetes by using stem cells to create insulin-producing cells (called beta cells) that could replace nonfunctional pancreatic cells. (sciencedaily.com)
  • While scientists have been able to change stem cells into insulin-producing cells, these cells don't have markers that indicate they are beta cells, and they aren't responsive to glucose. (pharmaceuticalintelligence.com)
  • When blood glucose levels rise, beta cells in the pancreas normally make the hormone insulin. (pharmaceuticalintelligence.com)
  • Insulin triggers cells throughout the body to take up sugar from the blood. (pharmaceuticalintelligence.com)
  • In type 2 diabetes, the most common form, tissues in the body lose their sensitivity to insulin, and pancreatic beta cells can't make enough insulin to keep glucose levels in check. (pharmaceuticalintelligence.com)
  • The cultured beta cells had specific markers that were found on normal beta cells which displayed changes in calcium levels when exposed to glucose and packaged insulin into granules. (pharmaceuticalintelligence.com)
  • However, when transplanted into mice these cells secreted insulin in response to glucose. (pharmaceuticalintelligence.com)
  • New research uses an innovative technique to convert human stem cells into insulin-producing beta cells much more effectively. (medicalnewstoday.com)
  • The insulin-producing cells created 'rapidly cured' type 1 diabetes in mice, and the benefits lasted for 9 months. (medicalnewstoday.com)
  • Human stem cells, shown here, can turn into insulin-producing beta cells. (medicalnewstoday.com)
  • In type 1 diabetes, a faulty autoimmune response causes the immune system to attack and destroy insulin-producing beta cells within the pancreas. (medicalnewstoday.com)
  • He says, "A common problem when you're trying to transform a human stem cell into an insulin-producing beta cell - or a neuron or a heart cell - is that you also produce other cells that you don't want. (medicalnewstoday.com)
  • Some of these cells are insulin-producing beta cells. (medicalnewstoday.com)
  • These mice had very severe diabetes with blood sugar readings of more than 500 milligrams per deciliter of blood (mg/dL)- levels that could be fatal for a person - and when we gave the mice the insulin-secreting cells, within 2 weeks their blood glucose levels had returned to normal and stayed that way for many months. (medicalnewstoday.com)
  • Expression of Pdx-1, Glut-2 and Insulin genes in EX-4 treated cells was significantly higher than the cells exposed to differentiation media without EX-4. (celljournal.org)
  • The percentage of insulin positive cells in the EX-4 treated group was ap- proximately 4-fold higher than in the EX-4 untreated ADMSCs. (celljournal.org)
  • We conclude that by manipulating the composition of the culture medium it is possible to induce canine ADMSCs into insulin-producing cells, and the 2-staged protocol that was used promoted the best differentiation. (sdlifesciences.com)
  • Blood glucose, insulin levels, and survival of rats were monitored periodically following intravenous injection of the tested cells. (biomedcentral.com)
  • Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
  • The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells. (bvsalud.org)
  • The aim of our study is to determine whether insulin -producing cells (IPCs) differentiated from adipose- tissue -derived stem cells (ADSCs) can be cryopreserved. (bvsalud.org)
  • Stem cells are an extremely promising approach for developing many cell therapies, including better treatments for type 1 diabetes," says Salk Professor Juan Carlos Izpisua Belmonte, the paper's senior author. (sciencedaily.com)
  • Products from Novartis and Gilead proved it was possible to win regulatory approval for chimeric antigen receptor T cell (CAR-T) based therapies in 2017. (genengnews.com)
  • Commercial scale manufacturing of CAR-T cell therapies is a new problem that really only exists since the approval of Kymriah and Yescarta in 2017. (genengnews.com)
  • This is an order of magnitude improvement in instrument throughput, which will make desperately needed cell therapies more widely available for patients. (genengnews.com)
  • As a result, thousands of patients who could benefit from cell therapies are dying unnecessarily," says Gerlinghaus. (genengnews.com)
  • If so, it would be the first practical method for generating iPS cells that could be used for transplant therapies, he said. (foxnews.com)
  • Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)
  • As stem cell-based therapies move towards clinical application, it is clear that more relevant animal models are required to demonstrate the safety and efficacy of stem cells for specific human diseases. (ca.gov)
  • These diseases are characterized by the loss of specific cell types and can be treated and potentially cured with stem cell-based therapies. (ca.gov)
  • These diseases can be potentially improved or even cured by cell replacement-based therapies. (ca.gov)
  • Some clinics may falsely advertise that it is not necessary for FDA to review and approve their stem cell therapies. (cdc.gov)
  • Stem cells from dental tissues have a real potential in Advanced Therapies. (bvsalud.org)
  • Previous research has pointed to human pluripotent stem cells (hPSCs) as a potential therapeutic avenue for type 1 diabetes. (medicalnewstoday.com)
  • Once generated, we will use these rat models to assess the safety and therapeutic potential of cells derived from human pluripotent stem cells. (ca.gov)
  • Cell replacement using human embryonic stem cells (hESCs) derivatives may offer new therapeutic opportunities to replace degenerated neurons and repair damaged circuits. (lu.se)
  • Conclusions: The data support the long-term therapeutic potential of ESC-derived human striatal progenitor grafts for the replacement of degenerated striatal neurons in HD and suggest that EE can effectively accelerate the maturation and promote the integration of human striatal cells. (lu.se)
  • 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
  • The potential therapeutic use of stem cells has been broadly researched in recent years. (bvsalud.org)
  • The stem cells from exfoliated deciduous teeth (SHEDs) have a great potential for therapeutic use because of its differentiation capability and its easy access, since the collection is performed at the physiological exfoliation stage of the deciduous teeth. (bvsalud.org)
  • In this way, cells from tissues such as the brain, heart, or pancreas can be created and observed in the laboratory, enabling safe testing for new disease treatments before starting clinical trials. (medindia.net)
  • These cells - capable of generating all cell types in the body - could be used as the 'lego bricks' to build tissue constructs, larger structures of tissues, and potentially even micro-organs. (phys.org)
  • These cells, which can be derived from adult tissues (most often the skin), have the potential to become any kind of cell found in the adult body. (sciencedaily.com)
  • Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
  • To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
  • The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
  • Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
  • 8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. (hindawi.com)
  • Until now, several progenitor cells derived from dental tissues have been isolated and characterized ( table I ). (bvsalud.org)
  • Adult stem cells are found in differentiated tissues and are able to generate specialized cells in some types of tissues (Multipotent Stem Cells). (bvsalud.org)
  • With stem cell associated techniques (SCAT) the BioRescue scientists aim to overcome this bottleneck: By using stored and preserved tissue of rhino skin it should be possible in principle to create induced pluripotent stem cells (iPCSs), primordial germ cells and finally artificial gametes. (izw-berlin.de)
  • They established culture systems for the southern white rhino, for which embryonic stem cells are available, and the northern white rhino, for which they used induced pluripotent stem cells derived from tissue samples. (izw-berlin.de)
  • However, obtaining primary neuronal cells from adult tissue is difficult and faces major ethical issues in clinical practice. (hindawi.com)
  • Normally, adult, tissue-specific stem cells are held in a kind of cellular deep freeze called quiescence to avoid unnecessary cell division in the absence of injury. (stanford.edu)
  • Rodgers and his colleagues found that a protein called hepatocyte growth factor, which exists in a latent form in the spaces between muscle cells and tissue, can activate a critical signaling pathway in the cells by binding to their surfaces. (stanford.edu)
  • That method would introduce a risk of cancer to any patient who got transplanted tissue derived from the iPS cells . (foxnews.com)
  • Kathrin Plath of the University of California, Los Angeles, called the work "very impressive" and said it appears to show the best approach so far for making such cells for transplant tissue. (foxnews.com)
  • In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
  • Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
  • Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
  • Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
  • For stem cells to be used in tissue engineering a scaffold is essential to provide the necessary support for the transport of nutrients, oxygen and the elimination of metabolic waste 30 , promoting a conducive environment for cell growth and differentiation. (bvsalud.org)
  • Scientists were able to produce from human embryonic stem cells a highly pure, large quantity of functioning neurons that will allow them to create models of and study diseases such as Alzheimer's, Parkinson's, prefrontal dementia and schizophrenia. (sciencedaily.com)
  • However, the percentage of neurons in the cell culture was not high and the neurons were difficult to isolate from the other cells. (sciencedaily.com)
  • UCLA's Yi Sun, an associate professor of psychiatry and biobehavioral sciences, and Howard Hughes Medical Institute investigator Thomas Südhof at the University of Texas Southwestern Medical Center were able to produce 70 to 80 percent of neurons in cell culture. (sciencedaily.com)
  • We're excited because we have been able to purify so many more neurons out of the cell culture and they were, surprisingly, healthy enough to form synapses. (sciencedaily.com)
  • When the time was right, Sun's team added protein growth factors into the cell culture that stopped the neural stem cells from self-renewing and prodded them into differentiating into neurons. (sciencedaily.com)
  • The neurons could then be separated from the neural stem cells that had not yet differentiated, a sort of chemical round-up that isolated the neurons. (sciencedaily.com)
  • The cells were then put into a cell strainer that allowed passage through of the isolated neurons. (sciencedaily.com)
  • The large number of pure neurons produced will allow Sun and her team to study their biological form and structure, the genes they express, the development of synapses and the electric and chemical communication activities within the synapse network. (sciencedaily.com)
  • Huntington's disease, for example, is a forebrain disease, so the neurons should be differentiated from a cell line that is biased to produce neurons from the forebrain. (sciencedaily.com)
  • Sun and her team confirmed that the two embryonic stem cell lines were different through gene expression analysis -- neurons that perform different functions in different parts of the brain express different genes. (sciencedaily.com)
  • Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
  • The brain tends to produce new neurons during the developmental years to help build neural circuits. (express.co.uk)
  • Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
  • Reprogrammed cells could then develop into a multitude of cell types, including the neurons found in the brain and spinal cord. (livescience.com)
  • This cell line will be used for the generation and purification of motor neurons. (ca.gov)
  • Nevertheless, S7 cells rapidly reversed diabetes in mice within 40 days, roughly four times faster than pancreatic progenitors. (nih.gov)
  • By that definition, the study created mouse-rat chimeras by introducing cells made up of rat genetic material inside the testes of mice. (iflscience.com)
  • In addition, one still needs to showcase the production of female reproductive cells (i.e., eggs) in female sterile mice, especially if we envision utilizing this technology for species conservation efforts. (iflscience.com)
  • In sexually reproducing organisms such as humans, rhinos or mice, primordial germ cells (PGCs) are embryonic precursors of sperm and eggs that pass on genetic and epigenetic information from one generation to the next. (izw-berlin.de)
  • The scientists relied on knowledge from the mouse model: In 2016, Katsuhiko Hayashi and his team managed to create primordial germ cell-like cells and finally germ cells from mice that were fertilised in the lab and resulted in healthy offspring being born. (izw-berlin.de)
  • After the cells were created, they were transplanted into a mouse model of type 1 diabetes, The model mice had a modified immune system that would not reject transplanted human cells. (sciencedaily.com)
  • Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
  • Two research teams cook up recipe to make long-sought cells in mice and people. (lifeboat.com)
  • However, when the cells were transplanted into diabetic mice, abnormally high blood glucose levels lowered. (pharmaceuticalintelligence.com)
  • Millman and team transplanted "islet-sized aggregates" of beta cells differentiated from hPSC into mice with type 1 diabetes. (medicalnewstoday.com)
  • The new stem cell protocol "can rapidly cure preexisting diabetes in mice," they emphasize later on. (medicalnewstoday.com)
  • We were able to make more beta cells, and those cells functioned better in the mice, some of which remained cured for more than a year. (medicalnewstoday.com)
  • Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells. (nature.com)
  • Androgenetic haploid embryonic stem cells produce live transgenic mice. (nature.com)
  • In a 2014 paper published in Nature , Rodgers and Rando showed in laboratory mice that an injury to the muscle of one leg caused a change in the muscle stem cells of the other leg. (stanford.edu)
  • A new study in mice shows that these melanocyte stem cells migrate between two sites in the hair follicle during each cycle of hair growth and shedding- from a site where they produce the pigment for hair color to another where they produce stem cells. (medicalnewstoday.com)
  • In this fashion, mice or other laboratory animals that exhibit particular traits can be created for specialized studies, or herds of farm animals (such as goats, sheep or cows) can be created that produce pharmaceutically useful proteins in their milk. (who.int)
  • The cells could potentially be used to fill the void left in the pancreas due to the damage that is caused by diabetes. (cellmedicine.com)
  • In the case of beta cells, we might get other types of pancreas cells or liver cells. (medicalnewstoday.com)
  • But if a quarter of the cells you make are actually liver cells or other pancreas cells, instead of needing a billion cells, you'll need 1.25 billion cells. (medicalnewstoday.com)
  • Pancreatic islets are groups of cells located in the pancreas. (medicalnewstoday.com)
  • In order to understand the role of SNPs in hereditary disease, scientists at Kyoto University's Center for iPS cell Research and Application (CiRA) create induced pluripotent stem cells from patient donors. (medindia.net)
  • The reprogramming produces what scientists call iPS cells, for induced pluripotent stem cells . (foxnews.com)
  • Now scientists have harvested such cells from the scalps and brain linings of human corpses and reprogrammed them into stem cells. (livescience.com)
  • Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
  • Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
  • Scientists are developing ways of enabling (inducing) other cells (such as a blood or skin cell) to act as stem cells. (msdmanuals.com)
  • The realization that not all human embryonic stem cell lines are born equal is critical," Sun said. (sciencedaily.com)
  • In the current work, the investigators started with human pluripotent stem cells (hPSCs). (sciencedaily.com)
  • Growing the cells in this way creates more shared surface area between the cells and allows them to influence each other, just as they would during human development. (sciencedaily.com)
  • Although human HSCs as vehicles to treat metachromatic leukodystrophy (MLD) has been used to treat patients with early onset MLD in a phase I/II trial, the HSCs give rise to all different blood cell lineages, such as the myeloid and lymphoid cell lineages [ 11 ]. (hindawi.com)
  • Transplanted human pancreatic cells from deceased donors have been successfully used to treat people with type 1 diabetes. (pharmaceuticalintelligence.com)
  • NEW ORLEANS - Retinal pigment epithelial (RPE) cells derived from human embryonic stem cells can be safely transplanted into the eyes of patients with retinal degeneration, with early signs of vision gain, according to pioneers in the field. (medscape.com)
  • Patients had the dry form of age-related macular degeneration (AMD) or Stargardt disease and received injections of human embryonic stem-cell (hESC)-derived RPE cells. (medscape.com)
  • In both studies presented here, human embryonic stem cells were turned into RPE cells and injected into the subretinal space of patients with retinal degeneration at a dose of 50,000 to 200,000 cells. (medscape.com)
  • Human embryonic stem cells have an endless capacity to divide, offer an unlimited source of cells, are capable of becoming any type of cell, and can be differentiated in the laboratory. (medscape.com)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • High-glucose Dulbecco's modified Eagle medium ( HG - DMEM ) supplemented with 20% fetal bovine serum followed by HG - DMEM with 2% horse serum is found to enable proliferation of both C2C12 muscle precursor cells and TE85 human osteosarcoma cells, fusion of C2C12s into myotubes, as well as an upregulation of RUNX2/CBFa1 in TE85s. (sdlifesciences.com)
  • The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
  • The main goal of this project is to develop rat models for assessing the safety and efficacy of human stem cell grafts. (ca.gov)
  • We proposed to use the classical embryonic stem cell-based gene-targeting technology to generate rat models mimicking human heart failure, diabetes and neurodegenerative diseases. (ca.gov)
  • Before human stem cells can be used in clinical trials, however, their safety and efficacy need to be tested in animal models. (ca.gov)
  • I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)
  • iPS cells retain the genetic makeup of the donor and can be converted into any cell type in the body. (medindia.net)
  • CRISPR/Cas9 has been heralded as a major breakthrough in genetic engineering to precisely make specific alterations to the DNA in cells and experimental animals. (medindia.net)
  • As such, the mouse host environment, which was sterile due to a genetic mutation, was still able to support efficient sperm cell production from a different animal species. (iflscience.com)
  • Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
  • Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
  • If it turns out to be a very efficient way of generating iPS cells without any genetic modification, then it would be a big advance," Wernig said. (foxnews.com)
  • Moreover, several studies have shown that cancer cells have genetic instability, epigenetic changes, and an accumulation of mutations, suggesting that cancer is a genetic disease [ 16 ]. (hindawi.com)
  • First, we introduced targeted genetic mutations into rat embryonic stem cells. (ca.gov)
  • Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
  • if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
  • However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
  • 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
  • The new approach is more efficient than earlier efforts and avoids tampering with DNA, said Derrick Rossi of Children's Hospital Boston and the Harvard Stem Cell Institute . (foxnews.com)
  • We're thrilled to have Chad Cowan, an associate professor at Harvard University who is at the Harvard Stem Cell Institute. (medscape.com)
  • This receptor, called (IL1RAP), cannot be found on healthy blood stem cells, which opens up the possibility for a selective attack on the diseased stem cells using antibodies. (lu.se)
  • IPCs induced in presence of EX-4 were morphologically similar to pancre- atic islet-like cells. (celljournal.org)
  • Ethical approval of the trial has already been obtained from the Swedish Ethics Review Authority, and the STEM-PD team, led from Lund University in Sweden, is thereby ready to proceed with the trial. (lu.se)
  • Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
  • Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
  • Ablative (myeloablative) treatment -- High-dose chemotherapy, radiation, or both are given to kill any cancer cells. (medlineplus.gov)
  • Stem cells are removed from you before you receive high-dose chemotherapy or radiation treatment. (medlineplus.gov)
  • After high-dose chemotherapy or radiation treatments, your stems cells are put back in your body to make normal blood cells. (medlineplus.gov)
  • Six patients demonstrated preretinal pigmented cells near the site of injection. (medscape.com)
  • They were able to produce a cell line that turned off certain genes to generate functional platelets. (ibtimes.com)
  • iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
  • While this combination is most conventional in producing iPSCs, each of the factors can be functionally replaced by related transcription factors, miRNAs, small molecules, or even non-related genes such as lineage specifiers. (wikipedia.org)
  • Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • Their technique treats skin cells with modified forms of RNA, a chemical cousin of DNA that normally transmits instructions from genes to the protein-making machinery of the cell. (foxnews.com)
  • This is a group of rare disorders caused by mutations in different genes involved in the development and function of infection-fighting immune cells. (clevelandclinic.org)
  • The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
  • One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
  • Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
  • The limitation in using stem cells to produce platelets has been the ability to find a method that creates a large number of high-quality, functional platelets. (ibtimes.com)
  • In order for them to be developed from stem cells, they need a very specific environment in which signals from hormones or proteins trigger the required morphological and functional transformation. (izw-berlin.de)
  • Because PGCs are the founder population for gametes, this accomplishment paves a way to produce functional gametes from induced pluripotent stem cells from northern white rhinos which will contribute to the effort to rewind their extinction. (izw-berlin.de)
  • This method for manufacturing large numbers of safe and functional beta cells is an important step forward. (sciencedaily.com)
  • The transplanted hPSC-derived beta cells were biologically functional. (sciencedaily.com)
  • Rando, who also directs Stanford's Glenn Center for the Biology of Aging , is the senior author of the study, published April 18 in Cell Reports . (stanford.edu)
  • Published in American Society for Cell Biology. (sciencemediacentre.org)
  • Diabetes clinical trials using embryonic stem cells are currently underway, but chronic immune suppression is required. (medindia.net)
  • More work is needed to develop these cells for clinical use. (pharmaceuticalintelligence.com)
  • Conditions such as Huntington's disease, Parkinson's disease, and spinal cord injuries are examples of clinical applications in which stem cells could offer benefits in halting or even reversing damage. (prnewswire.com)
  • In the late 1990s, sex-mismatched transplants and experiments with rodents revealed apparent transgressions of embryonic cell fates. (the-scientist.com)
  • Additionally, when these beta cells were tested in a mouse model of type 1 diabetes, the animals' blood sugar was brought under control within about two weeks. (sciencedaily.com)
  • Dr. Douglas Melton team from Harvard University funded in part by NIH's National Institute of Diabetes and Digestive and Kidney Diseases set out to transform stem cells into beta cells that have the potential to replace damaged beta cells. (pharmaceuticalintelligence.com)
  • In type 1 diabetes, the body's own immune system attacks and destroys beta cells. (pharmaceuticalintelligence.com)
  • One approach to treat diabetes is to replace destroyed beta cells. (pharmaceuticalintelligence.com)
  • Transplanting billions of such cells may soon cure type 1 diabetes. (medicalnewstoday.com)
  • You need about a billion beta cells to cure a person of diabetes. (medicalnewstoday.com)
  • The main goal of this project was to produce rat models of heart failure, diabetes, and amyotrophic lateral sclerosis. (ca.gov)
  • Using the small animal model embryonic stem cell-based gene-targeting technology that we developed, we will create small animal models for heart failure, diabetes and neurodegenerative diseases. (ca.gov)
  • Because stem cells multiply slowly, chemotherapy does not have a sufficient impact on them, which explains why two-thirds of these patients relapse. (lu.se)
  • In order for beta cell-based treatments to eventually become a viable option for patients, it's important to make these cells easier to manufacture," says co-first author Haisong Liu, a former member of the Belmonte lab. (sciencedaily.com)
  • While all medical treatments have benefits and risks, unapproved stem cell and exosome products expose patients to potential risks without a clear benefit. (cdc.gov)
  • Currently, the only stem cell treatments approved by the Food and Drug Administration (FDA) are products that treat certain cancers and disorders of the blood and immune system. (cdc.gov)
  • If you are considering stem cell treatments, check to make sure the product you are considering is on the FDA's approved list of stem cell treatments external icon . (cdc.gov)
  • Crucial to embryo production is the availability of oocytes (egg cells) and sperm. (izw-berlin.de)
  • However, since the generation of embryonic stem cells involves destruction (or at least manipulation) of the pre-implantation stage embryo, there has been much controversy surrounding their use. (wikipedia.org)
  • Polarization of hematopoiesis to the charter boat flooring in the bird embryo was proven to rely on the substitute of the preliminary aortic roofing by somite-derived endothelial cells (ECs) (Pardanaud et al. (researchdataservice.com)
  • The issue lies mainly in the specifics that: 1) credited to particular embryological restrictions in the mouse embryo, endothelium and sub-aortic mesenchyme are not really open to physical break up, and 2) both endothelium and sub-aortic mesenchyme are reported to exhibit the essential transcription aspect Runx1, producing the circumstance tough to evaluate (Azcoitia et al. (researchdataservice.com)
  • The sperm fertilizes the egg and the resulting cell divides, forming an embryo. (msdmanuals.com)
  • In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. (hindawi.com)
  • This was indicated by increased expression of stem cell markers (e.g. (cdc.gov)
  • CD44 and ABCG2) and fibrogenic markers (e.g., collagen and a-SMA) in CNT-exposed cells. (cdc.gov)
  • These animals, by large, appeared healthy and developed normally, although they carried both mouse and rat cells in a chimeric animal. (iflscience.com)
  • dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
  • Stem cell research and experimentation have been in process for well over five decades, as stem cells have the unique ability to divide and replicate repeatedly. (prnewswire.com)
  • The cytoskeleton is a structure that helps cells keep their shape and offers the mechanical support that allows cells to move, divide, and multiply. (medicalnewstoday.com)
  • Stem cells divide, producing more stem cells, until they are triggered to specialize. (msdmanuals.com)
  • Then as they continue to divide, they become more and more specialized until they lose the ability to be anything but one kind of cell. (msdmanuals.com)
  • Our previous study was the first to demonstrate 8-nitroguanine formation in the cancer cells of NPC patients via iNOS activation [ 14 ], showing that inflammation is an important risk factor for NPC development. (hindawi.com)
  • For many cancers, the donor's white blood cells may attack any remaining cancer cells, which are seen as foreign, similar to when white cells attack bacteria or viruses when fighting an infection. (medlineplus.gov)