• Polypyrimidine tract-binding protein 1 (PTBP1) is a RNA-binding protein (RBP) expressed throughout B cell development. (ox.ac.uk)
  • Deletion of Ptbp1 in mouse pro-B cells results in upregulation of PTBP2 and normal B cell development. (ox.ac.uk)
  • We show that PTBP2 compensates for PTBP1 in B cell ontogeny as deletion of both Ptbp1 and Ptbp2 results in a complete block at the pro-B cell stage and a lack of mature B cells. (ox.ac.uk)
  • In pro-B cells PTBP1 ensures precise synchronisation of the activity of cyclin dependent kinases at distinct stages of the cell cycle, suppresses S-phase entry and promotes progression into mitosis. (ox.ac.uk)
  • PTBP1 controls mRNA abundance and alternative splicing of important cell cycle regulators including CYCLIN-D2, c-MYC, p107 and CDC25B. (ox.ac.uk)
  • The RNA-binding protein, polypyrimidine tract-binding protein 1 (PTBP1), also named hnRNP I, acts as a critical mediator of insulin biosynthesis through binding to the pyrimidine-rich region in the 3'-untranslated region (UTR) of insulin mRNA. (molcells.org)
  • Here, we report that glucose induces the expression of PTBP1 via the insulin receptor (IR) signaling pathway in β cells. (molcells.org)
  • PTBP1 is present in β cells of both mouse and monkey, where its levels are increased by glucose and insulin, but not by insulin-like growth factor 1. (molcells.org)
  • PTBP1 levels in immortalized β cells established from wild-type (βIRWT) mice are higher than levels in β cells established from IR-null (βIRKO) mice, and ectopic re-expression of IR-WT in βIRKO cells restored PTBP1 levels. (molcells.org)
  • However, PTBP1 levels were not altered in βIRKO cells transfected with IR-3YA, in which the Tyr1158/1162/1163 residues are substituted with Ala. Consistently, treatment with glucose or insulin elevated PTBP1 levels in βIRWT cells, but not in βIRKO cells. (molcells.org)
  • Thus, our results identify insulin as a pivotal mediator of glucose-induced PTBP1 expression in pancreatic β cells. (molcells.org)
  • The researchers showed that as the cell senses low oxygen conditions, HIF-1 α begins regulating the RNA splicing factor, Polypyrimidine tract-binding protein 1 (Ptbp1), that in turn directs the splicing of the CEACAM1 gene, leading to the protective CEACAM1-S version that reduces the liver injury that accompanies transplantation. (technologynetworks.com)
  • Polypyrimidine tract-binding protein 1 (PTBP1) plays an essential role in splicing and is expressed in almost all cell types in humans, unlike the other proteins of the PTBP family. (bvsalud.org)
  • PTBP1 mediates several cellular processes in certain types of cells, including the growth and differentiation of neuronal cells and activation of immune cells. (bvsalud.org)
  • In this review, we summarize recent findings related to PTBP1 and discuss how it regulates the development of cancer cells. (bvsalud.org)
  • We investigated whether polypyrimidine tract-binding protein 1 (PTBP1), a novel regulator of cell proliferation and differentiation, is implicated in VSMC proliferation and neointima hyperplasia responding to injury. (bvsalud.org)
  • Two recent papers from Qian et al (4) and Zhou et al (5) demonstrate that reduced expression of the RNA binding protein PTB (encoded by Ptbp1-polypyrimidine tract-binding protein) allows the direct conversion of glial cells to neurons. (vectorbuilder.jp)
  • The primary microRNA (Pri-miRNA) is produced in the cell nucleus through the transcription of a DNA strand mediated by RNA polymerase II [ 2 ]. (archivesofmedicalscience.com)
  • A microRNA strand is transferred to the Argonaute complex (AGO), forming an RNA-induced silencing complex (RISC), and guides it to pair with the target mRNA through binding of the microRNA seed sequence with the microRNA recognition site on the mRNA. (archivesofmedicalscience.com)
  • However, the underlying mechanism that regulates its expression in β cells is unclear. (molcells.org)
  • 2006). Polypyrimidine tract binding protein regulates IRES-mediated gene expression during apoptosis. (nature.com)
  • Cul4a targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis. (biomedcentral.com)
  • These results suggest that GAPDH exerts other functions beyond glycolysis, and that oxidatively modified GAPDH regulates its cellular functions by changing its interacting proteins, i.e. the RNA splicing by interacting with the p54nrb-PSF complex. (elsevierpure.com)
  • SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling. (cas.cn)
  • Our results reveal a previously unrecognised mechanism mediated by a RBP that is essential for B cell ontogeny and integrates transcriptional and post-translational determinants of progression through the cell cycle. (ox.ac.uk)
  • In pancreatic β cells, glucose stimulates the biosynthesis of insulin at transcriptional and post-transcriptional levels. (molcells.org)
  • The regulation of expression of X-box-binding protein-1 (XBP1), a transcriptional factor, involves an unconventional mRNA splicing that removes the 26 nucleotides intron. (mdpi.com)
  • RNA-binding proteins (RBPs) mediate pivotal cellular functions such as RNA transport, degradation or translation and represent key effectors of post-transcriptional gene regulation. (nature.com)
  • It is an important way in post transcriptional regulation in eukaryotic cells. (chinbullbotany.com)
  • In this scholarly study, we discovered with a cell tradition program that DJ-1 stimulates manifestation from the gene in the transcriptional level by association with RREB1 which the decreased CCK level in the serum from DJ-1-knockout mice in comparison to that from wild-type mice was noticed. (biodiversityhotspot.org)
  • RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)
  • Accumulating evidence highlights emerging functions of RBPs in the post-transcriptional regulation of inner ear development and hair cell function. (encyclopedia.pub)
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • Polypyrimidine tract binding protein and associated splicing factor (PSF) has diverse functions inside the cell such as transcriptional regulation, DNA repair, acts as nucleic acids binding protein and regulate replication and infectivity of different viruses. (biomedcentral.com)
  • Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
  • An extensive bioinformatics analysis of the coronavirus replicase proteins by Snijder et al. (pdfroom.com)
  • The coronavirus replication cycle begins with the translation of the 29-kb positive-strand genomic RNA to produce two large polyprotein species (pp1a and pp1ab), which are subse- quently cleaved to produce 15 or possibly 16 nonstructural proteins (nsp's) (11). (pdfroom.com)
  • According to the Jacob-Monod-Lwoff paradigm, established in bacteria and logically extended to eukaryotes, what made a cell a cell was the combinatorial turning on and off of genes. (biologists.com)
  • The SNL 76/7 cell line is derived from a mouse fibroblast STO cell line transformed with neomycin resistance and murine LIF genes. (appliedstemcell.com)
  • The expressions of key genes and proteins of PI3K/Akt/Beclin1 signaling pathway in brain tissue were detected by qRT-PCR and Western blot respectively. (bvsalud.org)
  • We previously sought out genes whose manifestation was transformed in DJ-1-knockdown cells in comparison to that in parental cells with a DNA microarray, and we determined many applicant genes, like the cholecystokinin (CCK) gene [17]. (biodiversityhotspot.org)
  • The transcriptomic and epigenomic regulatory landscape of the MAC genome, which has 80% protein-coding genes and short intergenic regions, is poorly understood. (uni-frankfurt.de)
  • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. (biomedcentral.com)
  • Chemical shift changes upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a positively charged patch on the protein surface as the binding site responsible for the previously reported affinity for nucleic acids. (pdfroom.com)
  • Polypyrimidine tract-binding protein induces p19(ink4d) expression and inhibits the proliferation of h1299 cells. (biomedcentral.com)
  • In order to obtain more detailed information regarding the interaction between host proteins and the defined viral nucleic acid and/or proteins, we investigated the host factors associating with the main replicase enzyme, NS5, in the JEV-infected cells using yeast-two hybrid screening. (biomedcentral.com)
  • The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. (biomedcentral.com)
  • The integration process is catalyzed by viral protein integrase which utilizes different host proteins for integration of viral DNA [ 1 ]. (biomedcentral.com)
  • RNA-binding proteins (RBPs) have essential roles in RNA-mediated gene regulation, and preliminary data suggest that they might regulate ES cell fate. (bvsalud.org)
  • By combining bioinformatic analyses with functional screening, we identified seven RBPs played important roles for the exit from pluripotency of ES cells. (bvsalud.org)
  • These data uncover the critical role of RBPs in pluripotency exit and suggest the application of targeting RBPs in controlling ES cell fate. (bvsalud.org)
  • RNA-binding proteins (RBPs) exert a broad range of biological functions. (nature.com)
  • To explore the scope of RBPs across eukaryotic evolution, we determined the in vivo RBP repertoire of the yeast Saccharomyces cerevisiae and identified 678 RBPs from yeast and additionally 729 RBPs from human hepatocytic HuH-7 cells. (nature.com)
  • Only 60% of yeast and 73% of the human RBPs have functions assigned to RNA biology or structural motifs known to convey RNA binding, and many intensively studied proteins surprisingly emerge as RBPs (termed 'enigmRBPs'), including almost all glycolytic enzymes, pointing to emerging connections between gene regulation and metabolism. (nature.com)
  • Recently developed unbiased high content techniques to identify RBPs in vivo yielded information on differences in cell type-specific expression and/or RNA-binding activity of RBPs in mammalian cells 2 , 3 . (nature.com)
  • Here we identify a large set of RBPs that are conserved between yeast and human cells. (nature.com)
  • LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
  • There are three major isoforms of BAG-1 in mammalian cells, termed BAG-1L (p50), BAG-1M (p46) and BAG-1S (p36) that function as pro-survival proteins and are associated with tumorigenesis and chemoresistance. (nature.com)
  • viral proteins may decoy some host factors for assisting viral replication. (biomedcentral.com)
  • Objective: To study the expression and effect of small nuclear ribonucleoprotein-associated protein B (SNRPB) on proliferation and metastasis of liver cancer tissues and cells. (bvsalud.org)
  • In the model group, nerve cells of brain tissue were loosely packed, interstitial edema, triangular in shape, nuclear pyknosis and dark-blue staining were observed. (bvsalud.org)
  • Subcellular fractionation further indicated that GAPDH remained relatively constant in the cytosol, while increasing at 12 to 24 hours postinfection (hpi) and decreasing at 36 hpi in the nuclear fraction of infected cells. (biomedcentral.com)
  • A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
  • We identified the interacting proteins of oxidized inactive GAPDH as p54nrb (54 kDa nuclear RNA-binding protein) and PSF (polypyrimidine tract-binding protein-associated splicing factor), both of which are known to exist as heterodimers and bind to RNA and DNA. (elsevierpure.com)
  • Its function is regulated by various molecules, including microRNAs (miRNAs), long non-coding RNAs (lncRNAs), and RNA-binding proteins. (bvsalud.org)
  • To address the second, we investigated RNAs bound by the metabolic enzyme hydroxysteroid dehydrogenase 17-β 10 (HSD17B10). (nature.com)
  • After cell lysis, polyadenylated RNAs were captured on oligo d(T) beads followed by stringent washes to remove non-crosslinked proteins. (nature.com)
  • Total RNAs had been ready from cells and put through semi-quantitative RT-PCR analyses. (biodiversityhotspot.org)
  • instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of JEV by electrophoretic mobility shift assays. (biomedcentral.com)
  • Accordingly, GAPDH binds to the minus strand more efficiently than to the plus strand of JEV RNAs. (biomedcentral.com)
  • qRT-PCR and immunoblotting were used to determine the mRNA and protein expression levels, respectively. (bvsalud.org)
  • The expression of SNRPB mRNA and protein in liver cancer cell lines were analyzed by qRT-PCR and Western blot. (bvsalud.org)
  • siRNA-SNRPB had significantly inhibited the expression of SNRPB mRNA and protein in liver cancer cells. (bvsalud.org)
  • The response of cells to environmental signals, as well as their differentiation, death or malignant transformation, involves changes in gene expression. (biologists.com)
  • We conclude that reduced U2AF(65) binding is a molecular consequence of disease-relevant mutations, and that a structure-guided U2AF(65) variant is capable of manipulating gene expression in eukaryotic cells. (rcsb.org)
  • In contrast, the redistribution patterns of GAPDH were not observed in the uninfected cells. (biomedcentral.com)
  • GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is known as a major target protein in oxidative stresses and becomes thiolated in its active site. (elsevierpure.com)
  • The C-terminal of p54nrb binds to GAPDH in the cytosol in amanner dependent on the dose of hydrogen peroxide. (elsevierpure.com)
  • The GAPDH-p54nrb complex enhances the intrinsic topoisomerase I activation by p54nrb-PSF binding. (elsevierpure.com)
  • Mutations in cis-acting splicing elements or changes in the activity of constitutive or alternative splicing could have a profound regulatory proteins that compromise the accuracy of either impact on human pathogenesis, in particular in tumor development and progression. (biologists.com)
  • The regulatory circuitry underlying embryonic stem (ES) cell self-renewal is well defined, but how this circuitry is disintegrated to enable lineage specification is unclear. (bvsalud.org)
  • Müller-McNicoll M, Rossbach O, Hui J , Medenbach J. Auto-regulatory feedback by RNA-binding proteins. (cas.cn)
  • Opposing roles of miR-294 and MBNL1/2 in shaping the gene regulatory network of embryonic stem cells. (cas.cn)
  • hnRNPLL depletion thus leads to sustained expression of ES cell-preferred isoforms, resulting in a differentiation deficiency that causes developmental defects and growth impairment in hnRNPLL-KO mice. (bvsalud.org)
  • RNA isoforms influence cell identity and function. (biorxiv.org)
  • Using enhanced long-read single-cell isoform sequencing, we comprehensively analyze RNA isoforms in multiple mouse brain regions, cell subtypes, and developmental timepoints from postnatal day 14 (P14) to adult (P56). (biorxiv.org)
  • However, human brains have evolved additional cell-type specificity in splicing, suggesting gain-of-function isoforms. (biorxiv.org)
  • They play major roles in the tissue- and stage-specific expression of protein isoforms as well as in the maintenance of protein homeostasis. (encyclopedia.pub)
  • We show that this conserved RNA interactome harbours many proteins without previously assigned roles in RNA biology (enigmRBPs), including surprisingly many metabolic enzymes. (nature.com)
  • It is estimated that microRNAs regulate production of approximately 60% of all human proteins and enzymes that are responsible for major physiological processes. (archivesofmedicalscience.com)
  • We characterized hnRNPLL, which mainly functions as a global regulator of alternative splicing in ES cells. (bvsalud.org)
  • Further, it has been shown that polypyrimidine tract binding (PTB) protein acts as an important regulator of p53 IRES activities. (iisc.ac.in)
  • PNC's are present in ovarian cancer cells and treatment with ML246 decreases invasion in vitro and tumor growth and spread in vivo. (biomedcentral.com)
  • Different splice variants of a given protein can display different and even antagonistic biological functions. (biologists.com)
  • Purine interruptions of polypyrimidine (Py) tract splice site signals contribute to human genetic diseases. (rcsb.org)
  • The essential splicing factor U2AF(65) normally recognizes a Py tract consensus sequence preceding the major class of 3' splice sites. (rcsb.org)
  • It binds the 3′ AG splice acceptor dinucleotide of the pre-mRNA target intron, and forms a heterodimer with U2AF2 , which binds the adjacent polypyrimidine tract. (massgenomics.org)
  • Normally, cyclin interacts with cyclin-dependent kinase (CDK) to form a cyclin-CDK complex, which promotes cell cycle progression, whereas cyclin-dependent kinase inhibitor (CDKI) molecules inhibit the formation of cyclin-CDK complex, arresting cell cycle. (biomedcentral.com)
  • Inhibition of notch signaling by gamma secretase inhibitor engages the rb pathway and elicits cell cycle exit in t-cell acute lymphoblastic leukemia cells. (biomedcentral.com)
  • ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. (thermofisher.com)
  • Initiation of BAG-1 protein synthesis can occur by both cap-dependent and cap-independent mechanisms and it has been shown that synthesis of BAG-1S is dependent upon the presence of an internal ribosome entry segment (IRES) in the 5′-UTR of BAG-1 mRNA. (nature.com)
  • We have shown previously that BAG-1 IRES-meditated initiation of translation requires two trans -acting factors poly (rC) binding protein 1 (PCBP1) and polypyrimidine tract binding protein (PTB) for function. (nature.com)
  • 1999). Cellular stress in Xenopus kidney cells enhances the phoshorylation of eukaryotic initiation factor eIF(4E) and the association of eIF4F with poly(A)-binding protein. (nature.com)
  • One of the important mechanisms used for protein synthesis during these conditions is internal initiation. (iisc.ac.in)
  • Development , Journal of Cell Science , Journal of Experimental Biology , Disease Models & Mechanisms and Biology Open . (biologists.com)
  • Glial cells represent a model target since they are plentiful, proliferate in response to injury and most importantly, are highly plastic. (vectorbuilder.jp)
  • However, while the conversion of glial cells to functional neurons represents an attractive therapeutic approach for a number of neurodegenerative disorders, there has so far been limited success (3). (vectorbuilder.jp)
  • We found that, in the erythropoietin-induced, CD34-positive hematopoietic stem cell (HSC) differentiation system, knockdown of p19 INK4d delays terminal erythroid differentiation, inhibits erythroblast growth due to increased apoptosis, and leads to the generation of abnormally nucleated late-stage erythroblasts. (biomedcentral.com)
  • Specifically, hnRNPLL promotes multiple ES cell-preferred exon skipping events during the onset of ES cell differentiation. (bvsalud.org)
  • In the cochlea of mouse embryos, it is highly expressed in prosensory cells and down-regulated at the onset of hair cell differentiation. (encyclopedia.pub)
  • Prolonged expression of Lin28B delays prosensory cell cycle exit and prevents hair cell differentiation, suggesting that it functions to increase hair cell production [ 2 ] . (encyclopedia.pub)
  • Therefore, the antagonistic actions of Lin28B and let7 miRNA coordinate the timing of prosensory cell cycle withdrawal for hair cell differentiation. (encyclopedia.pub)
  • In neonatal murine cochlear organoids and explants, Lin28B antagonizes the activity of let7 miRNA and increases Akt-mTORC1 signaling to promote hair cell regeneration from immature supporting cells by inducing their de-differentiation and proliferation as well as by directly converting them into hair cells [ 3 ] . (encyclopedia.pub)
  • Thus, Lin28B functions in hair cell regeneration through mitotic and non-mitotic mechanisms, which are dependent on mitotic division or trans-differentiation of supporting cells into hair cells, respectively. (encyclopedia.pub)
  • To date, RUNX2 has been involved in diverse physiological processes, including osteogenic differentiation of mesenchymal stem/stromal cells, chondrocyte hypertrophy, immunomodulation, vascular invasion and endothelial cell migration via modulating a variety of signaling cascades (e.g. (spandidos-publications.com)
  • Conversely, we identified a preferred binding site of U2AF(65) for purine substitutions in the 3' regions of Py tracts. (rcsb.org)
  • Based on a comparison of new U2AF(65) structures bound to either A- or G-containing Py tracts with previously identified pyrimidine-containing structures, we expected to find that a D231V amino acid change in U2AF(65) would specify U over other nucleotides. (rcsb.org)
  • The most frequent site of alkylation in DNA is the O 6 position of guanine, which forms cross-links between adjacent strands of DNA, leading to cell death. (aacrjournals.org)
  • Unexpectedly, knockdown of p19 INK4d did not affect cell cycle, and these functions caused by p19 INK4d knockdown were via decreasing levels of GATA-binding protein 1 (GATA1). (biomedcentral.com)
  • However, as shown in our study, p19 INK4d played important roles independent of cell cycle regulation, and the lack of cell cycle change was probably due to the compensatory up-regulation of p18 INK4c following p19 INK4d knockdown. (biomedcentral.com)
  • Establishment of DJ-1-knockdown NIH3T3 (D2) and SH-SY5Y (KD4) cells was referred to previously [24], [25]. (biodiversityhotspot.org)
  • Knockdown of RREB1 was completed after transfection of RREB1 siRNA into NIH3T3 cells in 6-well plates by Lipofectamine 2000 (Invitrogen, Carlsbad, CA), as well as the CCK mRNA level was dependant on real-time PCR at 48 hrs after transfection. (biodiversityhotspot.org)
  • The siRNA knockdown (KD) of PSF resulted in increased viral replication in TZM-bl cells, suggesting PSF has negative influence on viral replication. (biomedcentral.com)
  • The quantitative PCR of virus infected PSF knockdown TZM-bl cells showed more integrated DNA and viral cDNA as compared to control cells. (biomedcentral.com)
  • This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • In conclusion, our study revealed the cell cycle-independent roles of p19 INK4d in human terminal erythropoiesis via a novel PEBP1- p ERK-HSP70-GATA1 pathway. (biomedcentral.com)
  • Interestingly, Lin28B inhibits the processing of mature let7 miRNA, which functions to induce cell cycle exit in progenitor cells [ 2 ] . (encyclopedia.pub)
  • Studies using a zebrafish model further illustrate an important role of Lin28A in the recovery of progenitor cells. (encyclopedia.pub)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a hallmark of vascular restenosis. (bvsalud.org)
  • P19ink4d is a tumor suppressor and controls pituitary anterior lobe cell proliferation. (biomedcentral.com)
  • The proliferation effect on hepatocellular carcinoma cells was observed by MTT assay. (bvsalud.org)
  • Conclusion: SNRPB expression is significantly increased in liver cancer tissues and cells, and it is involved in regulating the proliferation, metastasis and EMT of liver cancer cells. (bvsalud.org)
  • Alternative splicing is a crucial mechanism for generating protein diversity. (biologists.com)
  • The precise mechanism by which Lin28B and let7 miRNA regulate mTORC1 activity in cochlear epithelial cells awaits further investigation. (encyclopedia.pub)
  • This suggests that coactivation of Lin28B and Follistatin may represent an endogenous mechanism mediating reprogramming of supporting cells for hair cell regeneration. (encyclopedia.pub)
  • Identification of these interacting host cellular protein provide better understanding of mechanism of viral replication and subsequently development of new therapeutic approaches. (biomedcentral.com)
  • The probable mechanism of action of PTB protein has also been investigated. (iisc.ac.in)
  • Specifically targeting certain somatic cells circumvents immune recognition and bypasses the need to revert back to an embryonic state thus avoiding the loss of essential age-related and epigenetic factors. (vectorbuilder.jp)
  • Furthermore, we found that p19 INK4d modulates GATA1 protein levels through a novel pathway, the phosphatidylethanolamine-binding protein 1 (PEBP1)-phosphorylated extracellular signal-regulated kinase ( p ERK)-heat shock 70 kDa protein (HSP70)-GATA1 pathway [ 3 ]. (biomedcentral.com)
  • In addition, Lin28B functions to enhance the regenerative competence of maturing supporting cells in the cochlea through cooperation with Follistatin, which inhibits Lin28B-induced TGF-ß signaling that can trigger proliferative quiescence [ 5 ] . (encyclopedia.pub)
  • Proteins shown in red (FDR 0.01) represent the mRNA interactome. (nature.com)
  • 0.01) in liver cancer cells. (bvsalud.org)
  • Western blot was used to detect the changes of epithelial mesenchymal transition (EMT) markers in liver cancer cells after down-regulation of SNRPB expression. (bvsalud.org)
  • Western blot showed that the expression level of epithelial phenotype marker E-cadherin was decreased after down-regulation of SNRPB, while the expression levels of mesenchymal phenotype markers N-cadherin and vimentin was increased, suggesting that down-regulation of SNRPB inhibited EMT in liver cancer cells. (bvsalud.org)
  • ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
  • By means of oligonucleotide microarray and RNA interference, we reveal that the sensitizing effect of IFN-β was possibly due to attenuation of MGMT expression via induction of the protein p53. (aacrjournals.org)
  • d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
  • RNA interference technique (siRNA) was used to determine SNRPB protein expression down-regulation. (bvsalud.org)
  • Objective: To investigate the effects of Zhongfeng capsule on the autophagy-related proteins expression in rats with cerebral ischemia/reperfusion injury (CI/ RI), and to explore its neural protection mechanisms of the decoction. (bvsalud.org)
  • Interestingly, targeting WDR5 by siRNA and OICR-9429 could block IFN-γ-induced PD-L1 expression in PCa cells. (thno.org)
  • SKOV3 cells were xenografted orthotopically under the ovarian bursa of immunocompromised mice. (biomedcentral.com)
  • Over time, the percentage of RFP positive cells expressing mature neuronal markers increased in AAV-shPTB injected mice thus demonstrating astrocyte-to-neuron conversion. (vectorbuilder.jp)
  • In contrast to control treated mice, treatment of mice with AAV-shPTB was able to restore the number of neuronal cell bodies together with a robust restoration of striatal dopamine and activity-induced dopamine release. (vectorbuilder.jp)
  • Consistent with this activity, functional analyses in mice suggest that Lin28B plays an important role in hair cell development and regeneration. (encyclopedia.pub)
  • We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
  • This pathway, called alternative splicing, is an adaptation that cells use to boost their protein diversity in times of danger, inflammation, and injury. (technologynetworks.com)
  • As expected, splicing varies strongly between cell types. (biorxiv.org)
  • In an attempt to study the host interacting protein of IN, we have identified a new interacting host protein PSF which is a splicing factor and elucidated its role in integration and viral replication. (biomedcentral.com)
  • In the present study, we have identified a new HIV-1 integrase interacting protein, polypyrimidine tract binding protein and associated splicing factor (PSF) through pull down assay and co immunoprecipitation of IN with mammalian cell protein. (biomedcentral.com)
  • This binding site is similar to the ssRNA-binding site of the sterile alpha motif domain of the Saccharomyces cerevisiae Vts1p protein, although the two proteins do not share a common globular fold. (pdfroom.com)
  • For instance, a recent study by our group reported the facilitating effect of RUNX2 during aggressiveness and chemoresistance of TNBC cells via activating MMP1, which was significantly associated with poor prognosis ( 21 ). (spandidos-publications.com)
  • Its prevalence was high, particularly in metastatic tumors and metastatically transformed cancer cell lines, making it a potential pan-marker for metastatic progression. (biomedcentral.com)
  • For instance, RUNX2 is involved in the progression of various tumor types, such as osteosarcoma, renal cell carcinoma, gastric cancer and breast cancer ( 15 - 20 ). (spandidos-publications.com)
  • Of note, other studies have also indicated the involvement of RUNX2 in breast cancer stem cells (BCSCs) and breast cancer progression ( 22 , 23 ). (spandidos-publications.com)
  • Various reports implicated host cellular proteins as a key factor that either interact directly with HIV-1 integrase (IN) or get involved in the integration process of virus resulting in the modulation of integration step. (biomedcentral.com)
  • It is possible to convert one somatic cell type to another with the best-known example being the reprogramming of fibroblasts to induced pluripotent stem (iPS) cells by "Yamanaka factors" (1). (vectorbuilder.jp)
  • It is relatively well documented that transcription factors and signaling pathways are critically involved in the formation of inner ear structures and in the development of hair cells. (encyclopedia.pub)
  • For example, a word in an open reading frame (ORF) has a direct influence on the primary amino acid sequence of a protein and hence is under strong selective pressure. (biomedcentral.com)
  • Orthotopic implantation of SKOV3 cells resulted in growth of the tumor on the ovary as well as spread of tumor tissues outside of the primary site on organs into the abdominal cavity. (biomedcentral.com)
  • The recurrence and drug resistance of breast cancer are intractable due to the presence of breast cancer stem cells (BCSCs), which are adequate to initiate tumor formation and refractory to conventional remedies. (spandidos-publications.com)
  • Mutant allele frequencies were at 40-50% , suggesting that the mutation was present in most or all tumor cells. (massgenomics.org)
  • Metastasis: Standard-risk patients usually do not have evidence of metastasis, which means that the cancer cells have not spread from the primary tumor site in the cerebellum to other parts of the central nervous system (CNS) or outside the CNS. (neurosurgery.directory)
  • This means that the cancer cells have spread from the primary tumor site in the cerebellum to other areas within the CNS or even outside the CNS, such as the spinal cord or other parts of the body. (neurosurgery.directory)