Cell penetrating dendrimers. Medical search. Frequent questions

Furthermore, cell-penetrating peptides (CPP) have been known as attractive carrier having applications in drug delivery, gene transfer and DNA vaccination. (scienceopen.com)
Active targeting involves use of targeting ligands such as antibodies, peptides (e.g cell penetrating peptides) which bind specifically to receptors, epitopes over-expressed on target site. (trialx.com)

Among them, nanoparticles (NPs) such as dendrimers, polymeric NPs, metallic NPs, magnetic NPs and quantum dots have emerged as effective vaccine adjuvants for infectious diseases and cancer therapy. (scienceopen.com)
Cultures were exposed 48 h to 6 and 12 ng ml -1 of gold nanoparticles coated with mannose generation 0 polyamidoamine dendrimer. (elsevierpure.com)
We conclude that nanoparticles' deteriorating effect in algae is caused by interactions with the cell wall, causing an aggregation of cell culture, and not by nanoparticle penetration inside the cytoplasm. (elsevierpure.com)
One of the most important applications of nanotechnology in medicine involves use of nanoparticles to deliver drugs, and other therapeutic substances to specific types of cells (such as cancer cells). (trialx.com)
Due to their small size, nanoparticles can also penetrate the blood-brain barrier which is impervious to most therapeutic and imaging agents. (trialx.com)
The small size of nanoparticles allows them to interact readily with biomolecules on the cell surface (receptors) and within the cell, and thus allows better understanding of complex processes that govern the behavior of cells in their normal state and during the diseased state. (trialx.com)
The aim of this study was to evaluate the effect of shear stress on cellular behaviors of biocompatible and biodegradable nanoparticles to cancer cells (A549 cell lines) in a biomimetic microfluidic system. (preprints.org)
The cellular interactions between nanoparticles and cells in drug delivery should be carefully examined according to the physicochemical properties of nanoparticles such as the type of materials, size and mainly surface charge in a biomimetic microfluidic condition. (preprints.org)

Interfaces 2021 Oct 13;13(40):47313-47326, Dual-Functional Dendrimer Micelles with Glycyrrhizic Acid for Anti-Inflammatory Therapy of Acute Lung Injury. (bioimagingsystem.com)

Sticky water surfaces: Helix-coil transitions suppressed in a cell-penetrating peptide at the air-water interface. (mpg.de)
16: 5437-5449, Development of AE147 Peptide-Conjugated Nanocarriers for Targeting uPAR-Overexpressing Cancer Cells. (bioimagingsystem.com)

Transfection can be carried out using calcium phosphate (i.e. tricalcium phosphate), by electroporation, by cell squeezing, or by mixing a cationic lipid with the material to produce liposomes that fuse with the cell membrane and deposit their cargo inside. (wikipedia.org)

Using a parallel artificial membrane permeability assay, it was found that RMG8-8 is likely unable to penetrate the blood brain barrier. (mtsu.edu)
However, RMG9-11 demonstrated good permeability, indicating that it may be able to penetrate the blood brain barrier to treat dangerous neurological infections of fungi. (mtsu.edu)
The development of other blood-brain barrier (BBB)-penetrating prodrugs is expected to be explored. (encyclopedia.pub)

In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)

The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)
While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)
Efforts have been made to increase drug carrier targeting efficiency by attaching cell receptor-targeted ligands to the particle surface [ 7 ]. (biomedcentral.com)

Besides stimulating ECM production, IGF-1 can stimulate the proliferation and chondrogenic differentiation of mesenchymal stem cells (MSCs) [ 7 ]. (biomedcentral.com)

This result was attributed to the higher localization of Ce6 at the tumor sites to induce cancer cell apoptosis. (bvsalud.org)
however, the study of ultrafine Pt NCs is at the beginning stage and there are still plenty of challenges like synthesis of near-infrared (NIR) fluorescent Pt NCs, the explicit signal pathway of cell apoptosis, and attempt in diverse biological applications that need to be urgently tackled in future. (hindawi.com)
Using a genome-wide screening approach, we set to identify genes involved in dsRNA entry, sensing, and apoptosis induction in human cells. (cnrs.fr)
The cell viability, reactive oxygen species (ROS) levels, ratios of oxidized nicotinamide adenine dinucleotide to its reduced form (NAD + /NADH), apoptosis assays, and cellular uptake of E/PCF-NPs were determined on 4T1 cells. (dovepress.com)
Treatment with E/PCF-NPs under irradiation induced 4T1 cell apoptosis in approximately 93.6% cells. (dovepress.com)
The long-circulating and higher tumor accumulation of E/PCF-NPs resulted in complete ablation of breast tumor tissue through the enhanced photothermal effect by NIR laser irradiation-mediated cell apoptosis. (dovepress.com)

Transfection of animal cells typically involves opening transient pores or "holes" in the cell membrane to allow the uptake of material. (wikipedia.org)
Compared to Ce6, Ce6-incorporated AuNPs exhibited higher cellular uptake and cytotoxicity against cancer cells in an irradiation time-dependent manner. (bvsalud.org)
By actively targeting nanoscale drug delivery systems it is possible to minimize the uptake of anticancer agent by normal cells thus minimizing the side effects of therapy. (trialx.com)
Different nanoparticle properties as well as cell type, interaction with other cells and cellular environments also show significant effect on nanoparticle uptake and drug efficacy. (preprints.org)
The cellular uptake of C-GONs by A549 cells in the absence of shear stress revealed that the mean fluorescence intensity was slightly decreased compared to that in the presence of shear stress. (preprints.org)

iv) Production and Characterization of 3D cell culture models aimed for drug screening. (cienciavitae.pt)

We have used the information gathered from application of these tools to design nano-based therapeutics that can achieve region- and cell-specific targeting in the brain for improved neuroprotection in a variety of brain injury models. (uw.edu)

IL-2 and IFN-γ), immune checkpoint blockade (ICB) therapy (e.g., anti-PD-1/PD-L1 antibodies), and adoptive T-cell transfer (e.g., chimeric antigen receptor (CAR) T-cell therapy) [ 3 - 5 ]. (thno.org)

Some excipients increase drug permeability and residence time in ocular tissues by enhancing corneal permeability, either by modifying the continuity of the epithelium, changing cell-to-cell junctions or altering the lipid/protein components of cell membranes. (reviewofophthalmology.com)
In the case of genes, these can be delivered to cells, absorbed and replicated by the cell to produce the protein that they code for. (anat.org.au)

Other physical methods use different means to poke holes in the cell membrane: Sonoporation uses high-intensity ultrasound (attributed mainly to the cavitation of gas bubbles interacting with nearby cell membranes), optical transfection uses a highly focused laser to form a ~1 µm diameter hole. (wikipedia.org)
Nanoscale drug delivery systems have ability to cross cell membranes, thus drug can be delivered to specific organelles inside the cell. (trialx.com)

Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
Treatment with a single dose of CPC-IGF-1 effectively suppressed IL-1α-induced GAG loss and nitrite release and rescued cell metabolism and viability throughout the 16-day culture period, while free IGF at the equivalent dose was not effective. (biomedcentral.com)

The most widely used physical method is electroporation, where short electrical pulses disrupt the cell membrane, allowing the transfected nucleic acids to enter the cell. (wikipedia.org)
Even after arriving at their sites of action, prior to the release of their cargo and clearance, NPs must effectively penetrate through the cell membrane and navigate intracellular compartment networks to get to their subcellular mark. (biomedcentral.com)

to tumor cells. (scirp.org)
Most of them generally follow the same pathway to make immune-activated cytotoxic T lymphocytes (CTLs) abundantly infiltrate into tumor tissues and destroy tumor cells [ 6 ]. (thno.org)
Of note, the TME is rich in immunosuppressive cells (e.g., tumor-associated macrophages (TAMs), regulatory T cells (Tregs), immature dendritic cells (iDCs) and myeloid-derived suppressor cells (MDSCs)), that can suppress antitumor immunity as a result of secretion of various cytokines and chemokines, which enables tumor escape from immune surveillance [ 8 ]. (thno.org)
Among most immunosuppressive cells, TAMs, as crucial drivers of immunosuppressive TME, account for the largest proportion of immune cells in the TME (approximately 50% of tumor mass) [ 9 , 10 ], which possess highly heterogeneity and play a complex regulatory role in tumor immunity and immunotherapy due to helping tumor evade immune surveillance [ 11 ]. (thno.org)

Immunotherapy is currently considered as a promising next-generation therapeutic strategy for various cancers thanks to its ability to modulate cell-specific immune responses toward tumors [ 1 ], which has gradually remodeled the landscape of clinical anticancer modality [ 2 ]. (thno.org)
15 as well as anticancer agents into cells. (hifsignaling.com)

This allowed us to determine that the heparan sulfate pathway and the conserved oligomeric Golgi complex are key determinants allowing entry of both dsRNA and viral nucleic acid leading to cell death. (cnrs.fr)

Because the term transformation had another sense in animal cell biology (a genetic change allowing long-term propagation in culture, or acquisition of properties typical of cancer cells), the term transfection acquired, for animal cells, its present meaning of a change in cell properties caused by introduction of DNA. (wikipedia.org)
Drug targeting is important in chemotherapy, a drug delivery system should target only the cancer cells while shielding the healthy cells from the drug. (trialx.com)
29 the cancer cells. (hifsignaling.com)
Nanomaterials for targeted drug delivery to cancer stem cells. (docksci.com)
Recent developments in cancer biology have identified the existence of a sub-poplulation of cells - cancer stem cells (CSC) that are resistant to most traditional therapies (e.g. chemotherapy and radiotherapy) and have the ability to repair their damaged DNA. (docksci.com)
One of the possible reasons for the failure of the present therapeutic regimens is the existence of a relatively rare cell population known as cancer stem cells (CSCs) that are resistant to or not targeted by present therapeutic modalities. (docksci.com)
Additionally, it is a possible carcinogenic, which triggers the growth of cancer cells. (athomespaday.com)

There are many different methods of gene delivery developed for various types of cells and tissues, from bacterial to mammalian. (wikipedia.org)
In contrast, near-infrared (NIR) light (650-950 nm) displays negligible phototoxicity and can penetrate more deeply into biological tissues (1-3.5 mm) [ 37 ]. (biomedcentral.com)
The holy grail of nano medicine would be to engineer the rebuilding of damaged cells and tissues at an atomic and molecular level, for example, for bone and neural reengineering, or the growth of organs for implantation. (anat.org.au)

Nanosized structures and devices are smaller than human cells which are around 10,000 nm in diameter and similar in size to biomolecules such as enzymes, proteins (hemoglobin is 5 nm in diameter). (trialx.com)
Rotaxane dendrimers with hyperbranched macromolecular interlocked structures and size modulation capacity demonstrate drug binding and release ability upon external stimuli. (edu.hk)
3D bioprinting is the process of manipulating cell-laden bioinks to fabricate living structures. (preprints.org)

Particular importance here is the biological applications of Pt NCs such as the bioimaging of various cells as a preferred fluorophore in contrast to traditional fluorescent markers (e.g., organic dye, semiconductor quantum dots, and fluorescent proteins), the usage of Pt NCs-based antitumour drugs as a new class chemotherapeutics for malignant tumour therapy, and the utilization of antibacteria as an alternative of Ag-based antibacterial agent. (hindawi.com)

and magnetofection, where nucleic acids are attached to magnetic iron oxide particles and driven into the target cells by magnets. (wikipedia.org)

It gives a full rundown of the current NP-based vaccines, their potential as adjuvants, and the ways they can be delivered to cells. (researchgate.net)

The emergence of multifunctional nanomaterials can expectedly endow these therapeutic cargoes with high solubility, favorable pharmacokinetic distribution, cell-specific delivery, and controlled release. (thno.org)

Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. (scienceopen.com)
22 have a relatively low stability and delivery efficacy compared to polyethyleneimine (PEI),dendrimer, and liposome. (hifsignaling.com)

In this study, we employed a genome-wide CRISPR-Cas9 loss-of-function screen based on cell survival in order to identify genes implicated in the host response to dsRNA. (cnrs.fr)

Interaction of a Patterned Amphiphilic Polyphenylene Dendrimer with a Lipid Mono layer: Electrostatic Interactions Dominate. (mpg.de)

25 the CD44 cell surface receptor. (hifsignaling.com)
50. Gornalusse G., Zhang M., Wang R., Rwigamba E., Kirby A.C., Fialkow M., Nance E., Hladik F. and Vojtech L. HSV-2 infection enhances Zika virus infection of primary genital epithelial cells independently of the known ZIKV receptor AXL. (uw.edu)

Among those molecular cues, double-stranded RNA (dsRNA) is a very potent inducer of different reactions at the cellular level that can ultimately lead to cell death. (cnrs.fr)

CCC-003 suppressed cell proliferation in ALK-mutated neuroblastoma cells. (bvsalud.org)

Alternative therapies, including autologous chondrocyte implantation (ACI), matrix-induced ACI (MACI), and stem cell transplantation, are also available commercially or are in the clinical study phase. (biomedcentral.com)
Stem Cell Res Ther. (jefferson.edu)

The MSI analysis not only demonstrated its ability to image select rotaxane dendrimers in a rapid and efficient manner but also provided tremendous assistance on the applications of the further treatment of cancerous tissue as safe drug carriers. (edu.hk)
It is a thin layer of tissue that lines the back of the eye and contains millions of photoreceptor cells that convert light into electrical signals. (tohealthbridge.com)
He is lecturing Molecular Cell Biology, Biomaterials and Tissue Engineering subjects to the 1st and 2nd cycles of Biomedical Sciences, Biochemistry, Bioengineering, Biotechnology as well as to the Pharmaceutical Sciences MSc. (cienciavitae.pt)

In animal cells, transfection is the preferred term as transformation is also used to refer to progression to a cancerous state (carcinogenesis) in these cells. (wikipedia.org)
Successful, non-invasive delivery of biodegradable and non-toxic combination therapies that can target existing cancerous cells and prevent the development of new tumors all in one dose is ideal for clinical treatment. (ntno.org)

Two basic delivery approaches including physical delivery to achieve higher levels of antigen production and formulation with microparticles to target antigen-presenting cells (APCs) have demonstrated to be effective in animal models. (scienceopen.com)
Nanovaccines can improve antigen presentation, targeted delivery, stimulation of the body's innate immune system, and a strong T-cell response without putting people at risk. (researchgate.net)

Biological transfection is typically mediated by viruses, utilizing the ability of a virus to inject its DNA inside a host cell. (wikipedia.org)

For work with bacterial and archaeal cells transfection retains its original meaning as a special case of transformation. (wikipedia.org)

CPC-IGF-1 rapidly penetrated through the full thickness of cartilage creating a drug depot owing to electrostatic interactions with negatively charged aggrecan-glycosaminoglycans (GAGs). (biomedcentral.com)
However, most of the administered drug is rapidly cleared out through the lymphatics and synovium vasculature preventing them from reaching their cell and matrix target sites in cartilage [ 5 ]. (biomedcentral.com)

Chlorin-e6 (Ce6) incorporated onto the surface-engineered AuNPs exhibited higher cell incorporation efficiency than bare AuNPs. (bvsalud.org)
We showed that COG4 knockout led to a decrease of extracellular HS that specifically affected dsRNA transfection efficiency and reduced viral production, which explains the increased cell survival of these mutants.IMPORTANCE When facing a viral infection, the organism has to put in place a number of defense mechanisms in order to clear the pathogen from the cell. (cnrs.fr)

Compared to chemotherapy or photothermal treatment alone, the combined treatment in vitro significantly inhibited the survival rate of 4T1 cells to 17.7%, induced ROS generation, and reduced NAD + /NADH significantly. (dovepress.com)

Synthetic dsRNA is also able to mimic viral-induced activation of innate immune response and cell death. (cnrs.fr)
By challenging HCT116 human cells with either synthetic dsRNA or Sindbis virus (SINV), we identified the heparan sulfate (HS) pathway as a crucial factor for dsRNA entry, and we validated SINV dependency on HS. (cnrs.fr)

The exact cause of RP is unknown, but it is believed to be caused by genetic mutations that affect the production of proteins essential for photoreceptor cell function. (tohealthbridge.com)

Transduction is often used to describe virus-mediated gene transfer into eukaryotic cells. (wikipedia.org)

In this report, their interactions with algae were investigated using two strains of Chlamydomonas reinhardtii, a wild type having cell wall and a cell wall-deficient mutant. (elsevierpure.com)
Culture aggregation was found only for wild type cells, probably because of interactions between mannose and cell wall glycoproteins. (elsevierpure.com)

Hierarchical organization of the hemostatic response to penetrating injuries in the mouse macrovasculature. (jefferson.edu)

It was found that the identified type III RDs G1 and G2 penetrated through the tunica serosa and were predominantly localized in red pulp regions of spleens. (edu.hk)

The original meaning of transfection was "infection by transformation", i.e., introduction of genetic material, DNA or RNA, from a prokaryote-infecting virus or bacteriophage into cells, resulting in an infection. (wikipedia.org)

Transfection can result in unexpected morphologies and abnormalities in target cells. (wikipedia.org)

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes1

Disciplines and Occupations1

Technology, Industry, Agriculture7

Peptides2

Nanoparticles8

Micelles1

Peptide2

Liposomes1

Blood brain b3

Polyamidoamine1

Ligands3

Mesenchymal stem1

Apoptosis6

Uptake5

Characterization1

Therapeutics1

Antibodies1

Protein2

Membranes2

Viability2

Membrane2

Tumor4

Anticancer2

Nucleic acid1

Cancer7

Tissues3

Structures3

Therapy1

Magnetic1

Adjuvants1

Pharmacokinetic1

Stability2

Genes1

Interaction1

Receptor2

Cellular1

Proliferation1

Stem Cell2

Tissue3

Cancerous2

Delivery2

Biological1

Bacterial1

Drug2

Efficiency2

Generation1

Synthetic2

Proteins1

Gene1

Interactions2

Response1

Found1

Infection1

Target1