Cell nucleus adult mammalian cells. Medical search. Frequent questions

However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
Our goals in studying mammalian iron-sulfur biogenesis are to understand how iron-sulfur prosthetic groups are assembled and delivered to target proteins in the various compartments of mammalian cells, including mitochondria, cytosol, and nucleus. (nih.gov)
The results show that the receptors were localised in the cytoplasm in myoblasts, in the nucleus in myotubes and in the extracellular matrix, in satellite cells and in the proximity of mitochondria in adult muscle fibres. (uea.ac.uk)
From these results we conclude that the non-genomic/non-canonical physiological functions of glucocorticoids, in adult skeletal muscle fibres are mediated by a glucocorticoid receptor localised in the extracellular matrix, in satellite cells and close to mitochondria and involve activation of the MAPK pathway. (uea.ac.uk)
[4] Mammalian erythrocytes also lose their other organelles such as their mitochondria . (wikidoc.org)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
This theory has its origins in the concept of "Wirkungssphäre" or "sphere of influence" proposed by Strassburger (1893) , in which he argued that a nucleus can only support a discrete volume of cytoplasm, thus defining the upper limits to cell size. (frontiersin.org)
We used isolated nuclei from virus infected cells supplemented with cytoplasm, and discovered that viral replicating chromosomes could continue replication in the absence of a nucleus. (nih.gov)
In Lowicryl-embedded sections of lung from adult rabbits, antisera (1:10,000) against the constitutive pulmonary microsomal cytochrome P-450 monooxygenase isozymes 2 and 5 and NADPH-cytochrome P-450 reductase (anti-2, anti-5 and anti-R) bound specifically to regions known to be rich in agranular endoplasmic reticulum (AER) in the cytoplasm of Clara cells. (aspetjournals.org)
Type I pneumocytes, ciliated cell cytoplasm, and nuclei were essentially unlabeled. (aspetjournals.org)
DNA viruses typically replicate in the host cell nucleus, and RNA viruses typically replicate in the cytoplasm. (msdmanuals.com)

Here we employ FISH methodology to track LINE-1 retrotransposition at the single nuclei level in chromosome spreads of HepG2 cell lines stably expressing synthetic LINE-1 . (jove.com)
The major advantage of this methodology is the fact that it allows the experimentalist to avoid over-or underestimation of rates and patterns of retrotransposition, and when complemented with other methodologies, allows for the study of L-1 retrotransposition at the single nuclei level. (jove.com)
Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nuclei profiling methods. (biomedcentral.com)

Viable offspring derived from fetal and adult mammalian cells. (springer.com)
Viable offspring derived from fetal and adult mam-malian cells. (chinagene.cn)
The birth of lambs from differentiated fetal and adult cells also reinforces previous speculation that by inducing donor cells to became quiescent it will be possible to obtain normal development from a wide variety of differentiated cells. (todayinsci.com)

Granulosa cells from adult sheep were heated to nonphysiological temperatures (55 degrees C or 75 degrees C) before their nuclei were injected into enucleated metaphase II oocytes. (cnrs.fr)
Furthermore, the nuclei of 90C95% granulosa cells of porcine MK-1775 manufacturer primordial, unilaminar, multilaminar, and antral follicles of different sizes stain positive for GATA4 [25]. (ap26113.com)
HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

One example of constitutive euchromatin that is 'always turned on' is housekeeping genes, which code for the proteins needed for basic functions of cell survival. (wikipedia.org)
Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
The very important question to be addressed at that time was whether all cell types in the body have the same set of genes. (biologists.com)
More than 30 mammalian genes comprise the TGFβ superfamily of growth factors. (nature.com)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
We evaluated the expression and activation of Notch pathway genes in the adult human and murine corneal epithelium during proliferation. (molvis.org)
In fact, amplification of genes by over replication of certain regions of DNA is one of the primary mechanisms by which cancer cells become resistant to drug therapy. (nih.gov)
It demonstrated that genes inactivated during tissue differentiation can be completely re-activated by a process called nuclear reprogramming: the reversion of a differentiated nucleus back to a totipotent status. (biomedcentral.com)
Dolly's creation showed that genes in the nucleus of a mature cell are still capable of reverting to an embryonic totipotent state - that is, the cell can divide to produce all of an animal's differential cells. (worldtimetodays.com)

Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
Fertilization of mammalian eggs is followed by successive cell divisions and progressive differentiation, first into the early embryo and subsequently into all of the cell types that make up the adult animal. (todayinsci.com)
Transfer of a single nucleus at a specific stage of development, to an enucleated unfertilized egg, provided an opportunity to investigate whether cellular differentiation to that stage involved irreversible genetic modification. (todayinsci.com)
The fact that a lamb was derived from an adult cell confirms that differentiation of that cell did not involve the irreversible modification of genetic material required far development to term. (todayinsci.com)
Enrichment of spermatogonial stem cells is important for studying their self-renewal and differentiation. (ap26113.com)
reported that GATA4 MK-1775 manufacturer localizes towards the coelomic epithelium of gonads also to the Sertoli and follicle cells before and after sex differentiation, [26] respectively. (ap26113.com)
In the process of erythroid differentiation, expressiosn�of both the transferring receptors CD71 and Ter119 were higher than that of adult blood cells, whereas, cytoskeletal-associated proteins (stathmin, septin8 and RBBP4) decreased gradually. (longdom.org)

It was clear that a definitive experiment required the replacement of a zygote nucleus by a somatic cell nucleus, asking whether the somatic nucleus could functionally replace the zygote nucleus by eliciting normal development of the enucleated recipient egg ( Fig. 1 )? (biologists.com)
I believe that the reprogramming errors are not the only cause of these low rates of cloning: the mammalian SCNT fails with a very high frequency mainly due to the damage that the technique itself inflicts in the egg and the somatic nucleus, and the very few successful cases occur only when the damage is not significant. (sibi.org)

Here we extend evidence in AS model mice ( Ube3a m -/ p + ) of paternal UBE3A expression within the suprachiasmatic nucleus (SCN), the master circadian pacemaker. (nature.com)
We previously observed persistent expression of UBE3A in the suprachiasmatic nucleus (SCN) of the hypothalamus, the master circadian regulatory region in the mammalian brain, of AS model mice 15 , thus identifying a novel site for relaxation of maternal expression bias of Ube3a in the adult brain. (nature.com)
Using the Cre-LoxP system, the mTOR gene was specifically knocked out in cells expressing Vip (vasoactive intestinal peptide), which represent a major population of interneurons widely distributed in the neocortex, suprachiasmatic nucleus (SCN), olfactory bulb (OB), and other brain regions. (concordia.ca)

The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (springer.com)
Briggs and King ( Briggs and King, 1952 ) had already succeeded in transplanting a blastula cell nucleus into an enucleated egg and obtaining normal tadpoles in the frog Rana pipiens . (biologists.com)
Even advanced donor cells from the endoderm of Xenopus tadpoles have nuclei that can sometimes yield normal individuals after nuclear transfer [data taken from Briggs and King ( Briggs and King, 1957 ) for Rana and from Gurdon ( Gurdon, 1962 ) for Xenopus ]. (biologists.com)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)

The latter findings point to the participation of Nogo-A/NgR1 signaling in the regulation of other aspects of growth, such as tissue expansion or turnover by cell proliferation. (jneurosci.org)
Failure of mammalian cells to regulate their proliferation cycle leads to cancer. (nih.gov)
Mammalian/mechanistic target of rapamycin (mTOR) signaling controls cell growth, proliferation, and metabolism in dividing cells. (concordia.ca)
2004) mTOR is essential for growth and proliferation in early mouse embryos and embryonic stem cells. (concordia.ca)
These total outcomes claim that GATA4-positive cells MK-1775 manufacturer support GDC development in two-dimensional tradition which, during tradition with GDCs, the GATA4-expressing FFCs most likely are likely involved similar compared to that of somatic cells in the testessupporting germ cell development and proliferation. (ap26113.com)

Why Cloning in Non-Human Mammalians Fail? (sibi.org)
Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)
Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Although attempts have not yet been made to create a therapeutic transplant from embryonic stem cells, the methods have been developed to allow the creation of functional, mature cells using human cell cloning technology. (news-medical.net)
Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
Dolly was the only surviving lamb from 277 cloning attempts and was created from a milk cell from a six-year-old Finn Dorset sheep. (worldtimetodays.com)

Viable Offspring Derived from Petal and Adult Mammalian Cells', Nature (1997), 385 , 810. (todayinsci.com)
When Professor Wilmut introduced the sheep in 1997, it paved the way for potential stem cell treatments to treat conditions such as Parkinson's disease, a degenerative disease that affects more than 150,000 people in the UK. (worldtimetodays.com)

Using a combination of biochemical, behavioral, and imaging approaches, we found that mice lacking mTOR in VIP neurons displayed erratic circadian behavior and weakened synchronization among cells in the SCN, the master circadian pacemaker in mammals. (concordia.ca)

The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
Most of these efforts are focused on donor cells. (biomedcentral.com)

Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
As a brand new graduate student starting in October 1956, my supervisor Michail Fischberg, a lecturer in the department of Zoology at Oxford, suggested that I should try to make somatic cell nuclear transplantation work in the South African frog Xenopus laevis . (biologists.com)

In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond. (cnrs.fr)
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
Signalling then converges to nuclear accumulation of transcriptionally active SMAD complexes and gives rise to a plethora of specific functional responses in both embryos and adult organisms. (nature.com)
My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)

Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
Dermal fibroblasts were taken from a 35-year old male and a 75-year-old male and used to create embryonic stem cells. (news-medical.net)

This is consistent with a negative-feedback loop, in which newly generated neurons modulate cell division of SVZ stem cells. (jneurosci.org)
An early study in mice showed that expression of Ube3a is nearly exclusively maternal in neurons within the CA3 region of the hippocampus and in cerebellar Purkinje cells, with moderate maternal bias in the cerebral cortex 6 . (nature.com)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)
Less is known regarding its function in postmitotic neurons in the adult brain. (concordia.ca)
In addition to the olfactory neurons, the epithelium is composed of supporting cells, Bowman glands and ducts unique to the olfactory epithelium, and basal cells that allow for the regeneration of the epithelium, including the olfactory sensory neurons. (medscape.com)

The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
However, Briggs and King ( Briggs and King, 1957 ) had also found that the nucleus of an endoderm cell from a neurula embryo could no longer support normal development ( Fig. 2 ). (biologists.com)

However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
TEM of a thin section of mammalian pancreatic tissue. (genomeweb.com)
Here, we compare gene expression and cellular composition of single-cell suspensions prepared from adult mouse kidney using two tissue dissociation protocols. (biomedcentral.com)
Multiple tissue preparation protocols are compatible with Chromium, but the protocol of choice should ideally maintain RNA integrity and cell composition of the original tissue. (biomedcentral.com)
In this case, samples need to be preserved either as an intact tissue or in a dissociated form as a single-cell suspension. (biomedcentral.com)
However, analysis of ultracellular fractions, isolated cell preparations, or light microscopic immunohistochemical studies of tissue sections has permitted only limited resolution of the distribution of this enzyme system within the 40 or more cell types of the lung. (aspetjournals.org)
Peripheral nerves contain non-neuronal cells and connective tissue as well as axons. (lookformedical.com)
Goblet cells exist within the simple columnar epithelial tissue. (freezingblue.com)

Now, maturing sample preparation methods are delivering more reliable single-nucleus RNA sequencing (snRNA-seq), letting researchers analyze cells in previously intractable tissues. (genomeweb.com)
In addition to PBMCs and embryonic and adult mouse brain, 10x customers have published snRNA-seq protocols and studies for a wide range of sample types, including human brain, kidney, and heart, a company spokesperson said in an email, as well as several tumor types. (genomeweb.com)
Even Martelotto, one of the foremost advocates of snRNA-seq in his new position as scientific director of Harvard Medical School's single-cell core, doesn't recommend it for every study. (genomeweb.com)
A 2013 study from researchers at the J. Craig Venter Institute and the Salk Institute was the first to hint that snRNA-seq was feasible as an alternative to a whole cell. (genomeweb.com)
Single-cell methods took off in the next few years, but it took until 2017 for snRNA-seq to arrive. (genomeweb.com)

Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Skeletal muscle is fairly unique in that the mature cells are syncytial and can contain hundreds of nuclei. (frontiersin.org)
A number of studies have previously proposed the existence of glucocorticoid receptors on the plasma membrane of many cell types including skeletal muscle fibres. (uea.ac.uk)
In this study, we investigated the localisation and the mechanism(s) underlying the non-genomic physiological functions of these receptors in mouse skeletal muscle cells. (uea.ac.uk)

When investigating the causes of this failure via cellular and molecular analysis of 2-cell zygotes and the successive cell divisions (blastomeres), all kinds of abnormalities were found. (sibi.org)

Daily haemoglobin oxidation rhythms are observed in mouse and human RBCs cultured in vitro, or taken from humans in vivo, and are unaffected by mutations that affect circadian rhythms in nucleated cells. (bvsalud.org)

Red blood cells have nuclei during early phases of development, but extrude them as they mature in order to provide more space for hemoglobin . (wikidoc.org)
The results of real-time monitoring of enucleation indicated that it took about 7 to 8 hours to extrude the nuclei from karyopyknosis (polychromatic erythroblasts). (longdom.org)

These results indicate that the two intestinal secretory peptides antagonistically regulate adult lifespan and intestinal senescence through multiple pathways, irrespective of insulin, which implicates a complementary gradient distribution of each of the hormone-producing EEs, consistent with local requirements for cell activity along the posterior midgut. (bioone.org)
Figure 2: Core signalling in the mammalian TGFβ-SMAD pathways. (nature.com)
The unique properties of human stem cells have aroused considerable optimism about their potential as new pathways for alleviating human suffering caused by disease and injury. (edu.au)

10x Genomics had a so-called "demonstrated protocol" for isolating peripheral blood mononuclear cell nuclei, so he tried throwing his pancreas samples into it. (genomeweb.com)

체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)
Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
It was created in a laboratory in Edinburgh in 1996 using a technique called somatic cell nuclear transfer (SCNT). (worldtimetodays.com)

This question had been asked by embryologists since 1886 ( Rauber, 1886 ), and Spemann ( Spemann, 1938 ) had demonstrated by an egg ligation experiment that the nuclei of an eight-cell frog embryo are developmentally totipotent. (biologists.com)

But for tissues that are hard to dissociate, or for samples with an unknown preservation history, "what you do is hit it with lysis, get the nuclei, and away you go," he said. (genomeweb.com)
Here, we present a novel biochemical assay for haemoglobin (Hb) oxidation status which relies on a redox-sensitive covalent haem-Hb linkage that forms during SDS-mediated cell lysis. (bvsalud.org)

The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
In vitro, the expression of Hes1 was higher in confluent cells maintained under high calcium conditions. (molvis.org)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)

Current research is focused on the mechanisms that regulate SMAD activity to evoke cell-type-specific and context-dependent transcriptional programmes. (nature.com)

By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)

To approach questions about the physiology of iron metabolism, we generated loss-of-function mutations of IRP1 and IRP2 in mice through homologous recombination in embryonic cell lines. (nih.gov)
The adult-onset neurodegeneration of adult IRP2 −/− mice is exacerbated when one copy of IRP1 is also deleted. (nih.gov)
GATA4 can be an integral regulator of Sertoli cell function in adult mice [21]. (ap26113.com)
Arsenic and arsenic compounds as a contaminant of drinking-water adult offspring in C3H/HeNCr mice together with various metals, spe- (IARC, 2004 ). (who.int)
2012) . In adult strain A/J mice, oral cluded that either moiety alone or er, non-arsenic-based compounds sodium arsenate increased the size some combination of both could be (IARC, 2004, 2012 ). (who.int)

This effect on longevity is apparently correlated with the midgut senescence phenotypes as a result of direct hormone action through both hormone receptors expressed in the enteroblasts or other midgut cell types. (bioone.org)
However, gut senescence does not appear to be the direct cause for longevity regulation, as knockdown of both hormone receptors did not affect adult lifespan. (bioone.org)

Phe is also found adsorbed to particulate matter and in the gas phase of air pollution, but to date, no studies have investigated the impact of Phe on mammalian cardiac function. (bvsalud.org)
OBJECTIVES: Our objectives were to determine the arrhythmogenic potential of acute Phe exposure on mammalian cardiac function and define the underlying mechanisms to provide insight into the toxicity risk to humans. (bvsalud.org)
DISCUSSION: To the best of our knowledge, this is the first evidence of direct inhibitory effects of Phe on mammalian cardiac electrical activity at both the whole-heart and cell levels. (bvsalud.org)

Firstly, the mammalian enzyme responsible for the synthesis of this linear biopolymer is unknown. (silverchair.com)
However, in contrast with what was previously demonstrated [ 9 ], we recently reported that D. discoideum cells lacking DdPpk1 have no detectable polyP levels demonstrating that this is the sole enzyme responsible for polyP synthesis in social amoeba [ 10 ]. (silverchair.com)

Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
Dolly was the first successfully created clone from an adult mammalian cell. (worldtimetodays.com)

But in some cases, especially cryopreserved or post-mortem tissues where some cells die or burst before others, analyzing nuclei leads to a less biased experiment, offering a more complete picture of the cell population at the time of sample collection. (genomeweb.com)
Even with tissues that can be prepared for single-cell sequencing with enzymes, "everything the cell feels is going to be represented in the transcriptome which is what we're using as cell readout," Martelotto said. (genomeweb.com)
Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. (biomedcentral.com)
Solid tissues need to be dissociated to release individual cells suitable for 10x Genomics Chromium scRNA-seq. (biomedcentral.com)
Red blood cells are the most common type of blood cell and the vertebrate body 's principal means of delivering oxygen from the lungs or gills to body tissues via the blood . (wikidoc.org)
In neonates, this area is a dense neural sheet, but, in children and adults, the respiratory and olfactory tissues interdigitate. (medscape.com)

And incubate for 90 minutes to arrest cells at metaphase. (jove.com)

These cells classified into several subtypes based on the hormones they produce in both mammals and insects. (bioone.org)
Erythrocytes in mammals are anucleate when mature, meaning that they lack a cell nucleus and as a result, have no DNA . (wikidoc.org)
In some other mammals such as dogs and horses, the spleen sequesters large numbers of red blood cells which are dumped into the blood during times of exertion stress, yielding a higher oxygen transport capacity. (wikidoc.org)
Unfortunately, the complete lack of information on the biosynthetic machinery of mammalian polyP has hampered our understanding of the physiological relevance of this polymer in mammals. (silverchair.com)

Using the same procedure, we now report the birth of live lambs from three new cell populations established from adult mammary gland, fetus and embryo. (todayinsci.com)

And startups such as Dolomite Bio and LevitasBio are keying in on nuclei isolation or cleanup as applications for their hardware. (genomeweb.com)

Oxygen can easily diffuse through the red blood cell's cell membrane . (wikidoc.org)
when lysed by pathogens such as bacteria, their hemoglobin releases free radicals that break down the pathogen's cell wall and membrane, killing it. (wikidoc.org)
The plasma membranes of bronchiolar Clara cells, the tips of microvillae of ciliated cells, secretory granules of goblet cells, and the cell membrane and pinocytotic vesicles of endothelial cells were all intensely labeled with anti-2 and anti-5 but not with anti-R, even at a 10-fold higher concentration. (aspetjournals.org)
all cells come in contact with the basement membrane (like alligator teeth). (freezingblue.com)

The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)

Cytoplasmic impact on cross-genus cloned fish derived from transgenic common carp ( Cyprinus carpio ) nuclei and gold-fish ( Carassius auratus ) enucleated eggs. (chinagene.cn)

Pluripotent teratocarcinoma-thymus somatic cell hybrids. (springer.com)
The resulting cells were pluripotent and could be differentiated into insulin-producing beta cells to restore the function of the pancreas in the donor. (news-medical.net)

English embryologist who in 1996 supervised the team of scientists that produced a lamb named Dolly, the first mammal cloned from a cell from an adult. (todayinsci.com)
He was a giant of the scientific world and led the Roslin Institute team that cloned Dolly the sheep - the first mammal cloned from an adult cell - which changed scientific thinking at the time. (worldtimetodays.com)

12] Lee KY, Huang HG, Ju BS, Yang ZG, Lin S. Cloned ze-brafish by nuclear transfer from long-term-cultured cells. (chinagene.cn)
The first offspring to develop from a differentiated cell were born after nuclear transfer from an embryo-derived cell line that had been induced to became quiescent. (todayinsci.com)

The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)

They have important roles in the regulation of cellular functions both in the adult organism and during embryogenesis. (nature.com)

However, their exact localisation and the cellular signalling pathway(s) they utilise to communicate with the rest of the cell are still poorly understood. (uea.ac.uk)
The intensity of labeling of AER in Clara cells with anti-R and anti-2, but not anti-5, appeared to correlate positively with the cellular content of secretory granules. (aspetjournals.org)
Once the provirus is integrated into the host cell DNA, it is transcribed using typical cellular mechanisms to produce viral proteins and genetic material. (msdmanuals.com)

Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
Cell Stem Cell. (springer.com)
2004) Disruption of the mouse mTOR gene leads to early postimplantation lethality and prohibits embryonic stem cell development. (concordia.ca)
Stem cell technology is the latest development in this controversial branch of science. (edu.au)
Embryonic stem cell technology is still at a preliminary research stage and announcements about its potential may be premature. (edu.au)
The ethical and legal controversies that were aroused in the ART debates during the 1980s have been re-ignited with the development of stem cell technology. (edu.au)
Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)
Stem cell technology in humans derives from earlier and complementary work in animal studies. (edu.au)
The subcultured GDCs in each passage experienced germ and stem cell characteristics, and circulation cytometric analyses exposed that ~60% of these cells were GFRPGP 9.5GFR-1NANOGwere used as shown in our earlier study [8]. (ap26113.com)
It has been reported that Nanog, Oct4, and Sox2, often used as stem cell markers, function cooperatively in the regulatory network of self-renewal and pluripotency [16]. (ap26113.com)
Based on these reports, it seems that germ and stem cell markers are expressed in cultured germ cells of domestic animals. (ap26113.com)
In this study, cells of the colonies at each passage were found to be positive for GFR em /em -1 and PGP 9.5, as well as for stem cell markers, in RT-PCR, immunocytochemistry, MK-1775 manufacturer and flow cytometry analyses. (ap26113.com)
These results suggest that the GDCs stably maintain germ and stem cell characteristics during subculture. (ap26113.com)

have shown that injection of kisspeptin antisera into the vicinity of the GnRH neuron cell bodies prevents the LH surge in both proestrous and ovariectomized estrogen-treated (OVX+E) rats. (jneurosci.org)
Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body. (lookformedical.com)

Consequently, we looked into the association between salivary degrees of dental histatin 5 and carriage prices by comparing kids and adults with DS and regular settings without DS to comprehend the physiological part of histatin 5 in age group- and gender-matched healthful individuals. (exposed-skin-care.net)
Rhythms are present in red blood cells (RBCs), the most abundant cell type in the body, but their physiological function is poorly understood. (bvsalud.org)

Enteroendocrine cells (EEs) are evolutionarily conserved gastrointestinal secretory cells that show scattered distribution in the intestinal epithelium. (bioone.org)

A nucleus contains only about 1 percent of total RNA inside a cell, but single-nucleus transcriptomics works because that 1 percent is enough to detect the majority of cell populations in a sample. (genomeweb.com)
It also reveals cell types more abundant either in the cold or warm dissociations that may represent populations that require gentler or harsher conditions to be released intact. (biomedcentral.com)

The canonical TGFβ signalling pathway involves ligand-dependent assembly of a heteromeric receptor complex, receptor-kinase activation and subsequent phosphorylation and activation of SMAD proteins, which are transcriptional regulators that consequently accumulate in the nucleus. (nature.com)
Paternal UBE3A-positive cells in the SCN show partial colocalization with the neuropeptide arginine vasopressin (AVP) and clock proteins (PER2 and BMAL1), supporting that paternal UBE3A expression in the SCN is often of neuronal origin. (nature.com)
In iron-depleted cells, the proteins bind to RNA stem-loops in transcripts known as iron-responsive elements (IRE). (nih.gov)

In addition to Martelotto's Frankenstein protocol, that year researchers at the Broad Institute from the lab of Aviv Regev, now head of Genentech Research, published DroNc-seq , a modified version of Drop-seq for nuclei. (genomeweb.com)
Researchers now say around 18% of healthy adults above the age of 50 face accelerated aging in at least one of their organs. (medicaldaily.com)
Researchers identified that an abundance of fungi in the gut, particularly strains of Candida albicans yeast, could trigger an increase in immune cells, which could worsen lung damage. (medicaldaily.com)

It is thought that the cell uses transformation from euchromatin into heterochromatin as a method of controlling gene expression and replication, since such processes behave differently on densely compacted chromatin. (wikipedia.org)

Chinese hamster ovary) cell suspension culture and purified by chromatography including specific viral inactivation and removal procedures. (who.int)

Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
So far the reprogramming of somatic cells shows very low rates of efficiency (~0.0006-1%) that have not improved in the last two decades of continuous research. (sibi.org)
Despite immense promise, somatic cell reprogramming still faces a critical challenge. (sibi.org)
It was believed that the epigenetic signature and age-related changes such as shortened telomeres and oxidative DNA damage might hinder reprogramming of mature adult nuclei. (news-medical.net)
These observations suggest that further studies on nuclear reprogramming are needed in order to understand the underlying mechanisms of reprogramming and significantly improve the ability of the differentiated somatic nuclei to be reprogrammed. (biomedcentral.com)

Cell Therapy for Parkinson's Disease: What Next? (lu.se)
These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)

In multiple studies in rodents, in- adults, but not in a dose-related um. (who.int)

The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)

Anatomy83

Organisms20

Diseases1

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes68

Disciplines and Occupations1

Information Science3

Health Care1

Mitochondria5

Cytoplasm6

Single nuclei3

Fetal and adult3

Granulosa Cells4

Genes10

Differentiation7

Somatic nucleus2

Suprachiasmatic nucleus3

Tadpoles4

Proliferation5

Cloning16

19972

Master circadian pacemaker1

Donor cells3

Transplantation2

Embryos9

Embryonic stem2

Neurons6

Endoderm2

Tissue8

SnRNA-seq5

Skeletal muscle4

Successive cell divisions1

Circadian1

Extrude2

Pathways3

Mononuclear1

SCNT5

Totipotent1

Lysis2

Vitro3

Regulate1

Genetically identical1

Mice5

Receptors2

Cardiac3

Enzyme2

Clone2

Tissues6

Metaphase1

Mammals4

Mammary1

Isolation1

Membrane4

Oocytes1

Eggs1

Pluripotent2

Mammal cloned2

Nuclear transfer2

Frogs1

Organism1

Cellular3

Stem Cell16

Neuron cell2

Physiological2

Intestinal epithelium1

Populations2

Proteins3

Researchers3

Chromatin1

Suspension1

Reprogramming5

Parkinson's2

Rodents1

Removed and transferred1