Cell's nucleusPulposusFound in eukaryotic cellsSingle-nucleiChromosomes inside the nucleusStructuresProteinsEukaryotic nucleusNeuronalSnRNA-seqBiologyInhibition2001LysisMicroscopic imagesEnters the nucleusProteinCellularMononuclearChromatinGenesMammalianMitochondriaTranscriptionalResearchersTumorsNeuronSegmentation algorithmEukaryotesLarge moleculesTissuesIsolationSomaticReagentsOrganismMoleculesTumorReplicateStem cellsDonorBiolBodiesGranuleReceptorsCerebellarHouses the genetic materialTypesOrganismsSuspensionsGene expressionNuclear pore complexesCytoplasm of the cellCorrelateMRNAViralStainHumanPeripheralEndoplasmic reticulumIntervertebral discDiploidSuspension cultureFractionMembrane-boundEpithelial cell
Cell's nucleus4
- These cells, in an image captured by scientist Chris Frick , Ph.D., are engineered with a label that marks the nuclear envelope, the thin membrane that surrounds the cell's nucleus. (alleninstitute.org)
- Researchers in the School of Computer Science's Computational Biology Department have developed a tool that aims to help scientists gain a fuller and deeper understanding of the structure and function of a cell's nucleus. (cmu.edu)
- Genomic data describes the makeup of the chromosomes inside a cell's nucleus and is typically represented as one- or two-dimensional data. (cmu.edu)
- Most DNA is inside a cell's nucleus. (medlineplus.gov)
Pulposus8
- The mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear. (medsci.org)
- Moreover, studies with human disc cells, especially focusing on nucleus pulposus (NP) cells, are seldom found in the literature. (medsci.org)
- A critical role of autophagy was identified in rat nucleus pulposus (NP) cells: Inhibition of autophagy suppresses, while activation of autophagy enhances, the catabolic effect of cytokines. (spandidos-publications.com)
- IVDs are composed of two distinct components: The inner gel-like core nucleus pulposus (NP) and the outer firm annulus fibrosus (AF). (spandidos-publications.com)
- Relatively little is known about cellular subpopulations in the mature nucleus pulposus (NP). (biomedcentral.com)
- The intervertebral disc (IVD) consists of a central gelatinous nucleus pulposus (NP) encircled by an elastic, ligamentous annulus fibrosus (AF) and is flanked superiorly and inferiorly by cartilaginous endplates. (biomedcentral.com)
- Delivering Growth Factors through a Polymeric Scaffold to Cell Cultures Containing both Nucleus Pulposus and Annulus Fibrosus. (bvsalud.org)
- and collagen type II (COL2A1) gene expressions were determined with quantitative real-time polymerase chain reaction (qRT- PCR ) analysis to show the effects of IGF-1 /BMP-2 administration on annulus fibrosus cell (AFC)/ nucleus pulposus cell (NPC) cultures . (bvsalud.org)
Found in eukaryotic cells2
- PL: nuclei) is a membrane-bound organelle found in eukaryotic cells. (wikipedia.org)
- Nucleus is specially found in eukaryotic cells. (topperone.com)
Single-nuclei1
- Multiple approaches for optimal sample dissociation and storage of single cells have been proposed as have single-nuclei profiling methods. (biomedcentral.com)
Chromosomes inside the nucleus1
- Each of our cells houses all its chromosomes inside the nucleus, as well as many specialized proteins that regulate how our genes switch on or off. (alleninstitute.org)
Structures8
- Inside the nucleus are forty-six threadlike structures known as chromosomes, and each one of these structures contains thousands of genes. (innerbody.com)
- The nuclear pore complex is one of the biggest protein structures in the cell,' explains Patrick Onck, professor of Micromechanics at the University of Groningen. (rug.nl)
- Biologists at the Allen Institute have further engineered these stem cells to tag certain proteins or cellular structures with glowing fluorescent labels. (alleninstitute.org)
- The movement of large molecules through the nuclear envelope and into the cell nucleus is regulated by large protein structures called nuclear pore complexes. (phys.org)
- The term nucleus is also used in zoology for a few other structures, such as the digestive organs of salpa. (sacklunch.net)
- Looking at the distribution of DNA in the nucleus, they discovered that much of the change in the viscoelastic nature of the nuclei is to do with the aggregation of chromatin, multi-component structures made of DNA and proteins. (scitechdaily.com)
- Structures that are part of or contained in the CELL NUCLEUS. (bvsalud.org)
- RESUME L'hématoxyline et l'éosine sont les colorations les plus couramment utilisées dans les laboratoires de pathologie pour la mise en évidence des structures cellulaires. (who.int)
Proteins12
- Although the interior of the nucleus does not contain any membrane-bound subcompartments, a number of nuclear bodies exist, made up of unique proteins, RNA molecules, and particular parts of the chromosomes. (wikipedia.org)
- 14 Embedded within the inner membrane, various proteins bind the intermediate filaments that give the nucleus its structure. (wikipedia.org)
- This size selectively allows the passage of small water-soluble molecules while preventing larger molecules, such as nucleic acids and larger proteins, from inappropriately entering or exiting the nucleus. (wikipedia.org)
- Histones are proteins that keep DNA strands curled up and packed into the cell nucleus. (sciencedaily.com)
- The pores are filled with special proteins, called nucleoporins, which select what can move in or out of the nucleus. (rug.nl)
- However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
- Both endogenous and enhanced green fluorescent protein (EGFP)-fused Gβ2 and Gγ2 proteins were detected in the nucleus at baseline, whereas a fraction of EGFP-Gβ2 and DsRed2-GR comigrated to the nucleus or the plasma membrane, depending on the exposure of cells to dexamethasone or somatostatin, respectively. (rupress.org)
- Even without stimulation, Forkhead is constantly moving into the nucleus, but is transported out again by export proteins. (broadinstitute.org)
- An array of holes, or pores, in the nuclear membrane allows for the selective passage of some certain molecules (such as proteins and nucleic acids) into and out of the nucleus. (topperone.com)
- It possesses DNA, which directs the formation of proteins and enzymes in the cell, it controls the gene expression and regulates other functions performed by the cell. (topperone.com)
- The nucleus is also responsible for the production of proteins, which are essential for a wide range of functions in the body. (the-one.world)
- Paternal UBE3A-positive cells in the SCN show partial colocalization with the neuropeptide arginine vasopressin (AVP) and clock proteins (PER2 and BMAL1), supporting that paternal UBE3A expression in the SCN is often of neuronal origin. (nature.com)
Eukaryotic nucleus3
- But they fail to portray the precise process underlying eukaryotic nucleus evolution. (sciencedaily.com)
- The origin of the eukaryotic nucleus must indeed be a milestone in the development of the cell itself, considering that it is the defining factor that sets eukaryotic cells apart from the other broad category of cells -- the prokaryotic cell. (sciencedaily.com)
- Bell proposed a viral origin for the eukaryotic nucleus back in 2001 and refreshed the theory in September. (uncommondescent.com)
Neuronal4
- Overall, snmC-seq allows for the large-scale multiplex sequencing of sorted neuronal nuclei that is needed to gain insight into the diverse cell types of the brain. (epigenie.com)
- SIGNIFICANCE STATEMENT A major hypothesis posits neuronal inhibitions in nucleus accumbens generate intense motivation. (jneurosci.org)
- Microinjections in nucleus accumbens of glutamate antagonist, DNQX, which might suppress local neuronal firing, generate either appetitive or defensive motivation, depending on site and environmental factors. (jneurosci.org)
- Is neuronal inhibition in nucleus accumbens required for such pharmacologically-induced motivations? (jneurosci.org)
SnRNA-seq4
- Now, maturing sample preparation methods are delivering more reliable single-nucleus RNA sequencing (snRNA-seq), letting researchers analyze cells in previously intractable tissues. (genomeweb.com)
- Even Martelotto, one of the foremost advocates of snRNA-seq in his new position as scientific director of Harvard Medical School's single-cell core, doesn't recommend it for every study. (genomeweb.com)
- A 2013 study from researchers at the J. Craig Venter Institute and the Salk Institute was the first to hint that snRNA-seq was feasible as an alternative to a whole cell. (genomeweb.com)
- Single-cell methods took off in the next few years, but it took until 2017 for snRNA-seq to arrive. (genomeweb.com)
Biology7
- In the recent decades, with exponential advancement in the fields of genomics, molecular biology, and virology, several scientists on this quest have taken to looking into the evolutionary twists and turns that have resulted in eukaryotic cells, the type of cell that makes up most life forms today. (sciencedaily.com)
- An adherent vessel preserves cell biology, but a cell suspension culture yields scale. (corning.com)
- S2 Genomics is developing integrated sample preparation systems for processing tissues into genomic samples for single-cell genomics and cell biology studies. (bucher.ch)
- It's a double-membraned organelle containing genetic material and other instructions required for celluar systems.Accordong to cell biology,the nucleus function is to act as the control center of the cell. (topperone.com)
- Cell biology breaks cell types into eukaryotic (those with a defined nucleus) and Prokaryotes(those with no defined nucleus). (topperone.com)
- Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
- Then our data becomes pestered with a lot of signals that have nothing to do with the biology at the time of harvesting those cells. (genomeweb.com)
Inhibition1
- Upon inhibition of nuclear export, FKHR accumulates in the nucleus. (broadinstitute.org)
20013
- In a way, Prof Takemura's hypothesis has its roots in 2001 when, along with PJ Bell, he made the revolutionary proposal that large DNA viruses, like the poxvirus, had something to do with the rise of the eukaryotic cell nucleus. (sciencedaily.com)
- The 2001 hypothesis by Prof Takemura and PJ Bell is based on striking similarities between the eukaryotic cell nucleus and poxviruses: in particular, the property of keeping the genome separate in a compartment. (sciencedaily.com)
- 5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
Lysis3
- Huang, S-H, Hung, L-Y & Lee, G-B 2016, ' Continuous nucleus extraction by optically-induced cell lysis on a batch-type microfluidic platform ', Lab on a Chip , vol. 16, no. 8, pp. 1447-1456. (edu.hk)
- But for tissues that are hard to dissociate, or for samples with an unknown preservation history, "what you do is hit it with lysis, get the nuclei, and away you go," he said. (genomeweb.com)
- In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)
Microscopic images2
- However, as the result of recent advancements in microscopic imaging technology and computational image processing techniques [ 1 ], there has been significant growth of research towards translational computational systems that can detect, analyze, classify, and quantify cell nuclei from microscopic images. (hindawi.com)
- To analyse the time-series 3D microscopic images of developing embryos with fluorescently labelled nuclei, these studies used image segmentation. (nature.com)
Enters the nucleus1
Protein12
- The protein labeled in these cells, known as lamin B1, is a key part of the nuclear envelope. (alleninstitute.org)
- Upon phosphorylation, R-SMADs recruit the co-SMAD SMAD-4 to form protein complexes that translocate into the nucleus and exhibit transcriptional activity. (springer.com)
- In addition, nuclear factor κB (NF‑κB), c‑Jun N‑terminal kinase (JNK), extracellular signal‑regulated kinases and p38 mitogen‑activated protein kinase inhibitors and TNF‑α were used to determine the molecular mechanism of autophagy during the inflammatory conditions, and only the NF‑κB and JNK inhibitor were found to enhance the autophagy of rat NP cells. (spandidos-publications.com)
- Here, we report that the guanine nucleotide-binding protein (G protein)-coupled receptor-activated WD-repeat Gβ interacts with the glucocorticoid receptor (GR), comigrates with it into the nucleus and suppresses GR-induced transactivation of the glucocorticoid-responsive genes. (rupress.org)
- This 96-well plate has images of cytoplasm to nucleus translocation of the Forkhead (FKHR-EGFP) fusion protein in stably transfected human osteosarcoma cells, U2OS. (broadinstitute.org)
- From the intricate mechanisms of DNA to the orchestration of protein synthesis, the animal cell with a nucleus is a testament to the remarkable achievements and progress of life. (the-one.world)
- Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
- A misfolded and aggregated isoform of a cell-surface protein termed cellular prion protein (PrP Sc ) is the main, if not the sole, component of prions ( 1 ). (cdc.gov)
- On a portion of thyroid cell facing the blood stream there is a protein called sodium iodine symporter that transports iodine into the thyroid cell. (cdc.gov)
- Phospholipid content was determined by extraction of cells with chloroform and methanol and protein and DNA were measured. (cdc.gov)
- Both messenger RNA (mRNA) and protein subunit COVID-19 vaccines work by delivering instructions (genetic material) to your cells to start building protection against the virus that causes COVID-19. (cdc.gov)
- Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. (lu.se)
Cellular8
- Accurate results of cell nuclei segmentation are often adapted to a variety of applications such as the detection of cancerous cell nuclei and the observation of overlapping cellular events occurring during wound healing process in the human body. (hindawi.com)
- We can see where cellular components are in the nucleus and how they interact with what's around them spatially. (cmu.edu)
- This triggered an increase in paracellular permeability via cellular redistribution of claudin 3 (CLDN3) from TJs into cell nuclei followed by disruption of epithelial integrity and formation of epithelial lesions. (springer.com)
- Single-cell RNA sequencing has been widely adopted to estimate the cellular composition of heterogeneous tissues and obtain transcriptional profiles of individual cells. (biomedcentral.com)
- Here, we compare gene expression and cellular composition of single-cell suspensions prepared from adult mouse kidney using two tissue dissociation protocols. (biomedcentral.com)
- Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
- Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
- Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
Mononuclear2
- 10x Genomics had a so-called "demonstrated protocol" for isolating peripheral blood mononuclear cell nuclei, so he tried throwing his pancreas samples into it. (genomeweb.com)
- Encephalitis: Infiltration with mononuclear cells, pycnosis of nuclei, ring infiltrates (nb mice). (cdc.gov)
Chromatin2
- In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond. (cnrs.fr)
- Megaloblasts are large nucleated red blood cell (RBC) precursors with noncondensed chromatin due to impaired DNA synthesis. (msdmanuals.com)
Genes8
- The genes within these chromosomes are structured in such a way to promote cell function. (wikipedia.org)
- The nucleus maintains the integrity of genes and controls the activities of the cell by regulating gene expression. (wikipedia.org)
- Independent phylogenetic analyses suggested that genes had been transferred between these viruses and eukaryotic cells as they interacted at various points down the evolutionary road, in a process called "lateral gene transfer. (sciencedaily.com)
- Further, unlike other viruses, it does not construct its own enclosed "viral factory" in the cytoplasm of the cell within which to replicate its DNA and contains none of the genes required to carry out the replication process. (sciencedaily.com)
- Its ability to perform cold dissociation minimizes the expression of stress-related genes in cells and helps preserve RNA quality in nuclei. (bucher.ch)
- It contains all of our genetic information and only genes that are necessary for skin cells are expressed.Thus, producing the unique cell type needed for the epidermis. (topperone.com)
- Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
- The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
Mammalian3
- Eukaryotic cells usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a few others including osteoclasts have many. (wikipedia.org)
- To our knowledge, this is the first report of cloned mammalian offspring originating from nonviable cells. (cnrs.fr)
- We previously observed persistent expression of UBE3A in the suprachiasmatic nucleus (SCN) of the hypothalamus, the master circadian regulatory region in the mammalian brain, of AS model mice 15 , thus identifying a novel site for relaxation of maternal expression bias of Ube3a in the adult brain. (nature.com)
Mitochondria2
- Growing evidence has persuaded some researchers, however, that the nucleus might have arisen through a symbiotic partnership much like the one believed to have produced mitochondria. (uncommondescent.com)
- However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
Transcriptional2
- Systematic comparison of recovered cell types and their transcriptional profiles across the workflows has highlighted protocol-specific biases and thus enables researchers starting single-cell experiments to make an informed choice. (biomedcentral.com)
- Each of the approaches mentioned above introduces specific biases and artifacts that can manifest themselves in altered transcriptional profiles or altered representation of cell types. (biomedcentral.com)
Researchers8
- The study, HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells , was the result of a collaboration of researchers from Touro University Nevada College of Osteopathic Medicine, Touro College of Osteopathic Medicine in New York, researchers from the Biotechnology Center (BIOTEC) of TUD Dresden University of Technology in Germany, and researchers from Italy. (israelnationalnews.com)
- Allen Institute for Cell Science researchers photograph thousands and thousands of different cells under the microscope. (alleninstitute.org)
- Researchers can get a more unbiased and complete view of how the cell nucleus is structured," Ma said. (cmu.edu)
- Researchers from Tokyo Metropolitan University have discovered how the sponginess and stickiness of stem cell nuclei controls how they "differentiate" into specialized cells. (scitechdaily.com)
- Researchers can now easily obtain suspensions of nuclei or high-viability cells for a wide range of single-cell analyses. (bucher.ch)
- The Singulator™ 100 enables rapid and hands-off tissue dissociations, making it easy for researchers to reproducibly prepare suspensions of nuclei or highly viable cells from small samples in high yield, for a wide range of single-cell analyses. (bucher.ch)
- For the first time, researchers have mapped the cell nucleus in 3D, revealing the packaging and organization of a cell's DNA in unprecedented detail. (lifeboat.com)
- In addition to Martelotto's Frankenstein protocol, that year researchers at the Broad Institute from the lab of Aviv Regev, now head of Genentech Research, published DroNc-seq , a modified version of Drop-seq for nuclei. (genomeweb.com)
Tumors3
- While some techniques only perform the task of cell nuclei segmentation and quantification, techniques that are capable of further detecting and classifying abnormal tumors (cell nuclei) that cause various types of cancer have also been proposed. (hindawi.com)
- S2 Genomics provides a selection of pre-set protocols and pre-formulated reagents for cell isolations for an expanding set of mouse, rat, and human tissues, including tumors. (bucher.ch)
- Circulating tumor DNA (ctDNA) is found in the bloodstream and refers to DNA that comes from cancerous cells and tumors. (medlineplus.gov)
Neuron2
- They calculated the CH methylation level for each neuron in non-overlapping 100-kb bins and clustered the cells according to their CH methylation profiles, where they annotated the clusters by known cell-type markers. (epigenie.com)
- 5 . Luthman J, Hoebeek FE, Maex R, Davey N, Adams R, De Zeeuw CI, Steuber V (2011) STD-dependent and independent encoding of input irregularity as spike rate in a computational model of a cerebellar nucleus neuron. (yale.edu)
Segmentation algorithm2
- A cell nuclei segmentation algorithm incorporating unsupervised color clustering, morphological operations, and local thresholding has been proposed to distinguish the cancerous and noncancerous areas in histologically stained images and then segment the clustered cell nuclei [ 3 ]. (hindawi.com)
- To quantify the nuclear region of every cell from a time-series 3D fluorescence microscopic image of living cells, we developed QCANet, a convolutional neural network-based segmentation algorithm for 3D fluorescence bioimages. (nature.com)
Eukaryotes2
- In eukaryotes the nucleus in many cells typically occupies 10% of the cell volume. (wikipedia.org)
- RBC and platelets present in eukaryotes also do not have the nucleus. (topperone.com)
Large molecules2
- These large molecules must be actively transported into the nucleus instead. (wikipedia.org)
- In the cell, large molecules that must be transported into or out of the nucleus can only do so when they are attached to such a chaperone. (rug.nl)
Tissues6
- Reproducibly generate high-quality suspensions of nuclei from a wide range of tissues. (bucher.ch)
- Solid tissues need to be dissociated to release individual cells suitable for 10x Genomics Chromium scRNA-seq. (biomedcentral.com)
- But in some cases, especially cryopreserved or post-mortem tissues where some cells die or burst before others, analyzing nuclei leads to a less biased experiment, offering a more complete picture of the cell population at the time of sample collection. (genomeweb.com)
- Even with tissues that can be prepared for single-cell sequencing with enzymes, "everything the cell feels is going to be represented in the transcriptome which is what we're using as cell readout," Martelotto said. (genomeweb.com)
- The pituitary gland produces thyroid stimulating hormone (TSH) that goes into the blood stream to activate thyroid cells, which then secrete T3 and T4 into the peripheral tissues. (cdc.gov)
- The nuclei of cells samples from the endometrium, appendix, are stained by its active product, haematin, gall bladder, spleen and other gastric and which is produced when an oxidizing agent pleural tissues obtained by surgery, curet- is added. (who.int)
Isolation2
- The Singulator 100TM System comes with a unique Single-Shot MechanismTM for convenient cell-isolation reagent loading and a Reagent Chiller that stores reagents for up to 100 nuclei runs. (bucher.ch)
- And startups such as Dolomite Bio and LevitasBio are keying in on nuclei isolation or cleanup as applications for their hardware. (genomeweb.com)
Somatic4
- Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. (cnrs.fr)
- Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
- The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
- if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
Reagents1
- The latter name is given to it from its property of absorbing staining reagents much more readily than the rest of the cell. (sacklunch.net)
Organism2
- Nucleus contains the genetic information for that organism. (topperone.com)
- Through a process called mitosis, cells can create exact copies of themselves, ensuring the growth and maintenance of the organism. (the-one.world)
Molecules7
- The nucleus is the headquarters of a cell and molecules constantly move across the nuclear membrane through pores. (rug.nl)
- The large membrance enclosed organelle which contains the genetic material in the form of multiple linear DNA molecules with the structure of chromosome is called cell nucleus. (topperone.com)
- Sometimes things like RNA need to traffic between the nucleus and the cytoplasm.There are pores in this nuclear membrane that allow molecules to go in and out of the nucleus. (topperone.com)
- It used to be thought that the nuclear membrane only allowed molecules to go out, but now it's realized that there is an active process also for bringing molecules into the nucleus. (topperone.com)
- Furthermore, the nucleus is like a vigilant gatekeeper, regulating the passage of molecules into and out of the cell. (the-one.world)
- He wanted to sequence RNA in single pancreas cells, but the organ, which produces digestive enzymes, was filled with RNases that could ruin the very molecules he was hoping to study. (genomeweb.com)
- The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)
Tumor6
- Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by tumor microparticles for virotherapy. (stembook.org)
- Ho CY, Maleki Z. Giant cell tumor of tendon sheath: cytomorphologic and radiologic findings in 41 patients. (ctsicn.org)
- As a tumor grows, cells die and are replaced by new ones. (medlineplus.gov)
- Analyzing the genome of tumor cells using ctDNA can help doctors determine which treatment will be most effective. (medlineplus.gov)
- Scientists have discovered that dying tumor cells release small pieces of their DNA into the bloodstream. (medlineplus.gov)
- These pieces are called cell-free circulating tumor DNA (ctDNA). (medlineplus.gov)
Replicate4
- A study published in the journal Nature Communications has identified a new pathway that human immunodeficiency virus (HIV) uses to enter the nucleus of a healthy cell, where it can then replicate and go on to invade other cells. (israelnationalnews.com)
- They must enter a "host" cell and use that cell's machinery to replicate its genetic material, and therefore multiply. (sciencedaily.com)
- What's truly astounding about the animal cell with a nucleus is its ability to replicate and divide. (the-one.world)
- HHVs replicate in the host cell nucleus. (medscape.com)
Stem cells6
- The cells these scientists work with are human stem cells, derived from an adult donor's skin cells and engineered to revert to a more "naive" state. (alleninstitute.org)
- In most stem cells, the nuclear envelope is smooth, but wrinkles can occasionally form in its thin surface - sometimes taking on unexpected shapes. (alleninstitute.org)
- How stem cells choose and keep to differentiation paths continues to be a crucial question for medical science. (scitechdaily.com)
- A team led by Associate Professor Hiromi Miyoshi of Tokyo Metropolitan University have been looking at the nuclei of human mesenchymal stem cells, a type of cell that can mature (or "differentiate") into a wide range of cell types, including muscle, fat, bone, and cartilage. (scitechdaily.com)
- This was the first time that the viscoelasticity of nuclei was tracked through the whole differentiation process in human stem cells. (scitechdaily.com)
- Reference: "Intranuclear mesoscale viscoelastic changes during osteoblastic differentiation of human mesenchymal stem cells" by Kojiro Matsushita, Chiharu Nakahara, Shun Kimura, Naoya Sakamoto, Satoshi Ii and Hiromi Miyoshi, 25 November 2021, The FASEB Journal . (scitechdaily.com)
Donor1
- Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Biol4
- J Cell Biol. (harvard.edu)
- J Cell Biol (2002) 157 (3): 533. (rupress.org)
- J Cell Biol (2005) 169 (6): 885-896. (rupress.org)
- Am J Respir Cell Mol Biol. (ctsicn.org)
Bodies4
- But what about individual cells in our bodies? (uncommondescent.com)
- There are certain bodies resembling cells in all respects except in the absence of a nucleus. (sacklunch.net)
- In neuroanatomy, a nucleus is a group of cell bodies of nerve cells in the brain or spinal cord. (topperone.com)
- Diagnosis is usually based on a complete blood count and peripheral smear, which usually shows a macrocytic anemia with anisocytosis and poikilocytosis, large oval red blood cells (macro-ovalocytes), Howell-Jolly bodies (residual fragments of the nucleus), hypersegmented neutrophils, and reticulocytopenia. (msdmanuals.com)
Granule2
- Individual granule (GR) cells exhibit diverse spiking patterns which are in-phase, anti-phase, or complex out-of-phase with respect to their population-averaged firing activity. (yale.edu)
- On the other hand, in botany, the nucleus may also refer to the central kernel of a nut or seed, or the center of a starch granule. (topperone.com)
Receptors2
- Extracellular stimuli that activate cell surface receptors modulate glucocorticoid actions via as yet unclear mechanisms. (rupress.org)
- The iodine hormone exerts its action on receptors on the nucleus in cells. (cdc.gov)
Cerebellar3
- 2 . Steuber V, Schultheiss NW, Silver RA, De Schutter E, Jaeger D (2011) Determinants of synaptic integration and heterogeneity in rebound firing explored with data-driven models of deep cerebellar nucleus cells. (yale.edu)
- 3 . Steuber V, Jaeger D (2013) Modeling the generation of output by the cerebellar nuclei. (yale.edu)
- An early study in mice showed that expression of Ube3a is nearly exclusively maternal in neurons within the CA3 region of the hippocampus and in cerebellar Purkinje cells, with moderate maternal bias in the cerebral cortex 6 . (nature.com)
Houses the genetic material1
- Among them, the nucleus houses the genetic material. (sciencedaily.com)
Types10
- The size of the nucleus is correlated to the size of the cell, and this ratio is reported across a range of cell types and species. (wikipedia.org)
- Ecker shares ,"We think it's pretty striking that we can tease apart a brain into individual cells, sequence their methylomes, and identify many new cell types along with their gene regulatory elements, the genetic switches that make these neurons distinct from each other. (epigenie.com)
- There are hundreds, if not thousands, of types of brain cells that have different functions and behaviors and it's important to know what all these types are to understand how the brain works. (epigenie.com)
- We identified changes of cell nuclei states caused by different types of cryoprotectants and associate them with alterations of the freeze/thaw process in cells. (muni.cz)
- What are the types of nucleus? (topperone.com)
- There are four types of nucleus. (topperone.com)
- It also reveals cell types more abundant either in the cold or warm dissociations that may represent populations that require gentler or harsher conditions to be released intact. (biomedcentral.com)
- However, optimal dissociation needs to achieve a balance between releasing cell types that are difficult to dissociate while avoiding damage to those that are fragile. (biomedcentral.com)
- Cell types that naturally have low RNA will be less represented. (genomeweb.com)
- Afin d'étudier l'effet de l'adoucissement de l'eau du robinet (extraction du calcium) sur les colorations à l'hématoxyline et à l'éosine, cinq (5) séries de lames issues de 30 blocs de tissus pathologiques humains différents inclus en paraffine ont été préparées de la même manière, sauf pour le lavage qui a été effectué avec cinq types d'eau différents. (who.int)
Organisms3
- The most widely accepted theories that have emerged state that the eukaryotic cell is the evolutionary product of the intracellular evolution of proto-eukaryotic cells, which were the first complex cells, and symbiotic relationships between proto-eukaryotic cells and other unicellular and simpler organisms such as bacteria and archaea. (sciencedaily.com)
- The animal cell with a nucleus, one of the greatest advancements in the realm of life, is like a sophisticated control center that drives the wonders of living organisms. (the-one.world)
- In approximately 20% of organisms only 1 nucleus is present. (cdc.gov)
Suspensions1
- The bench-top Singulator System and its single-use cartridges enable reproducible, rapid and hands-off tissue dissociations into single-cell or nuclei suspensions. (bucher.ch)
Gene expression3
- Join this webinar to learn more about the alteration of host gene expression as a result of COVID-19 infection, and novel laboratory techniques designed to evaluate this at a single-cell resolution. (bucher.ch)
- Single-cell RNA sequencing (scRNA-seq) is an increasingly powerful technology that enables analysis of gene expression in individual cells. (biomedcentral.com)
- We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. (lu.se)
Nuclear pore complexes1
- Maul GG, Deaven L. Quantitative determination of nuclear pore complexes in cycling cells with differing DNA content. (harvard.edu)
Cytoplasm of the cell1
- RAF-MEK-ERK signalling is normally thought to occur in the cytoplasm of the cell. (bath.ac.uk)
Correlate2
- Our interdisciplinary study reveals how changes in cell nuclei induced by cryoprotectants affect the ability of cells to withstand freezing and thawing and how nuclei changes correlate with processes during freezing and thawing. (muni.cz)
- The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)
MRNA2
- This study was designed to examine whether the unconventional spicing of XBP1 mRNA could occur in the nucleus and its possible biological relevance. (mdpi.com)
- We use RT-PCR reverse transcription system and the expand high fidelity PCR system to detect spliced XBP1 mRNA, and fraction cells to determine the location of the unconventional splicing of XBP1 mRNA. (mdpi.com)
Viral4
- HIV infection requires the virus to enter a cell and gain access to the well-guarded nucleus in order for the viral components to be integrated into the healthy cell's DNA. (israelnationalnews.com)
- He points to the "viral factories" that giant viruses build inside prokaryotic cells. (uncommondescent.com)
- To infect cells productively, HIV-1 must traverse the nuclear envelope to enable integration of the viral DNA into the genomic DNA of host cells. (phys.org)
- [ 5 , 6 ] In a localized primary infection, the virus penetrates the mucosal epithelium and invades the cells of the basal layer, where the viral DNA inserts into the host DNA. (medscape.com)
Stain2
- Nuclei are stained with DRAQ, a DNA stain. (broadinstitute.org)
- Five sections (5 µm thick) ity to stain nuclei without combining with a were prepared from each paraffin block mordant, such as aluminium, iron or other and placed on different slides. (who.int)
Human12
- 12 In human cells, the diameter of the nucleus is approximately six micrometres (µm). (wikipedia.org)
- Herein we elaborated effects of TGF-β1 on TJs of primary human bronchial epithelial cells. (springer.com)
- We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. (medsci.org)
- FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8 + T cells. (medsci.org)
- Finally, IKKβ knockdown was used to further confirm the effect of the NF‑κB signal on human NP cells autophagy, and the data showed that IKKβ knockdown upregulated the autophagy of NP cells during inflammatory conditions. (spandidos-publications.com)
- An example of a nucleus in real life is the nucleus of human skin cells. (topperone.com)
- In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
- NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
- June 8 (UPI) - The nucleus of the cell is where the action happens, but it's not easy to analyze the behavior of a massive genome inside an area 50 times smaller than the width of a human hair. (lifeboat.com)
- Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and in-tegrated into the genome of human cells. (lu.se)
- In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. (lu.se)
- Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. (lu.se)
Peripheral1
- We also collected macrophages and CD8 + T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. (medsci.org)
Endoplasmic reticulum1
- Furthermore, autophagy of AF cells may be secondary to endoplasmic reticulum stress ( 12 , 13 ). (spandidos-publications.com)
Intervertebral disc1
- Cell autophagy exists in degenerative diseases, including osteoarthritis and intervertebral disc degeneration. (spandidos-publications.com)
Diploid2
- Cells are normally diploid - this means that they have a pair - two sets of homologous chromosomes, and hence two copies of each gene or genetic locus. (topperone.com)
- 2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
Suspension culture2
- Cell Suspension Culture or Adherent Surface: Which Is Right for Your Lab? (corning.com)
- HeLa, mouse L, and CHO cells were grown in suspension culture. (harvard.edu)
Fraction2
Membrane-bound1
- The eukaryotic cell is neatly compartmentalized into membrane-bound organelles that perform various functions. (sciencedaily.com)
Epithelial cell1
- It is believed that TGF-β1 signalling is involved in controlling epithelial cell proliferation, to drive epithelial-mesenchymal transition (EMT) and it plays a major role in lung fibrosis and emphysema. (springer.com)