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  • chromosomes
  • Ag-staining of nucleolus organizer regions of chromosomes after A-,C-, G-, or R-banding procedures. (biomedsearch.com)
  • The cells were fixed in methanol:acetic acid (3:1 v/v), then dropped on cold, wet slides which were air-dried before storage at 4 degrees C. The slides were stained to identify the chromosomes by one of the following procedures: (1) Quinacrine. (biomedsearch.com)
  • Single-cell absolute contact probability detection reveals chromosomes are organized by multiple low-frequency yet specific interactions. (nih.gov)
  • interphase
  • G 1 (growth) G 0 G 2 (growth and final preparations for division) S (growth and DNA replication) M Cytokinesis T elophase Anaphase Metaphase Late prophase Prophase Interphase G 1 checkpoint Cell cycle main checkpoint. (slideshare.net)
  • HeLa
  • HeLa cells with compromised expression of the genes encoding lamins were analyzed using high-resolution imaging and pull-down assays. (biologists.org)
  • We localized these antigens by immunoelectron microscopy in DFC of HeLa cell nucleoli both on Lowicryl sections and cryoultrathin sections without embedding. (biologists.org)
  • In human peripheral lymphocytes, or HeLa cells treated with actinomycin D, the antigens were still present but the fluorescence intensity decreased. (biologists.org)
  • Nuclear
  • To test the links between structure and function, we evaluated how nuclear lamins contribute to the organization of a major functional compartment, the nucleolus. (biologists.org)
  • Since deletion of host NCL/nucleolin negatively impact on nuclear egress, UL24 supposedly acts on this process through its effect on host nucleoli. (uniprot.org)
  • These findings suggest a dual role for Tip5's AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure. (uni-regensburg.de)
  • Humans
  • SUMMARY ANSWER Three subtypes of marmoset undifferentiated spermatogonia, which were not evenly distributed in terms of number and position along the basal membrane, and an extra premeiotic cell division not present in humans were identified using HRLM. (deepdyve.com)
  • protein
  • Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinson's disease, on cell motility. (biologists.org)
  • Prof. Antebi from the Max Planck Institute for Biology of Ageing in Cologne notes that "the nucleolus contains a protein highly conserved in evolution called fibrillarin, which also decreases during infection. (scitechdaily.com)
  • Immunoblotting revealed reduced protein levels of all components of the γ-tubulin small complex (γ-tubulin, GCP2, and GCP3) in HCA66-depleted cells. (biologists.org)
  • To study changes at the centrosome during the cell cycle, we compared the protein composition of the pericentriolar material from unsynchronized Jurkat cells (66% in G1 phase, 25% in S phase, as verified by flow cytometry), and from Jurkat cells arrested in S phase by a double aphidicolin block (85% in S phase, 10% in G1). (biologists.org)
  • We recently showed that Hand1 is sequestered in the nucleoli of rodent trophoblast stem (TS) cells by the I-mfa domain-containing protein HICp40 and that this is associated with their proliferation and continuing self-renewal. (ox.ac.uk)
  • Hanakahi, Dempsey, Li, Maizels: Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1. (antibodies-online.com)
  • Ehrlich
  • Reference : Ultrastructural distribution of histones within Ehrlich tumor cell nucleoli: a cytoch. (uliege.be)
  • en] We investigated the ultrastructural distribution of histones within Ehrlich tumor cell nucleoli by means of three cytochemical methods and by a Lowicryl post-embedding immunogold labeling procedure involving anti-histone (H2B, H3, H4) antisera as well as antibodies to synthetic peptides of histones. (uliege.be)
  • Mitotic
  • This event underlies the release of Hand1 from the nucleolus and represents the 'molecular switch' that promotes mitotic cell cycle exit and the onset of endoreduplication. (ox.ac.uk)
  • fibrillarin
  • When we reduce fibrillarin in the nucleolus it is sufficient to defend against bacterial infection. (scitechdaily.com)
  • On the other hand, Antebi and his colleagues could show that activities that expand the nucleolus and increase fibrillarin make cells more sensitive to infection. (scitechdaily.com)
  • This paper details the advantages of using C. elegans to investigate features of the nucleolus during the organism's development by following dynamic changes in fibrillarin (FIB-1) in the cells of early embryos and aged worms. (nih.gov)
  • 2017
  • How to make a plant cell model for 7th grade science" last modified May 13, 2017. (ehow.co.uk)
  • Centrosome
  • To investigate changes at the centrosome during the cell cycle, we analyzed the composition of the pericentriolar material from unsynchronized and S-phase-arrested cells by gel electrophoresis and mass spectrometry. (biologists.org)
  • The centrosome constitutes a major microtubule-organizing centre in animal cells. (biologists.org)
  • indicate
  • These results appear to indicate that all the DNA detected within the nucleolus was associated with histones. (uliege.be)
  • These results indicate that the zygote NPBs are not used in embryonic development and that the nucleoli in developing embryos originate from de novo synthesized materials. (biologists.org)
  • regulation
  • This integrated transcriptomic analysis uncovers new unexpected roles for nucleolin in metabolic regulation and signaling pathways paving the way to better understand the global function of nucleolin within the cell. (antibodies-online.com)
  • nuclei
  • Sex in eukaryotes is a composite process, consisting of meiosis and fertilization (or, more generally, 'mixis', the process of fusion of cells and nuclei), which can be coupled to reproduction [ 3 ]. (royalsocietypublishing.org)
  • organelles
  • Within cells are various parts called organelles. (ehow.co.uk)
  • These organelles each have a unique function, such as ridding the cell of waste or providing the cell with necessary nutrients. (ehow.co.uk)
  • Plant cells and animal cells share many of the same organelles. (ehow.co.uk)
  • It is best to place the vacuole off to one side of the cell so that it will be easier to fit the rest of the organelles. (ehow.co.uk)
  • biogenesis
  • With normal lamin expression, the nucleoli were highly plastic, with dramatic and freely reversible structural changes correlating with the demand for ribosome biogenesis. (biologists.org)
  • To begin addressing these problems, we have compared the mobilities of factors involved in various steps of ribosome biogenesis in living cell nucleoli. (rupress.org)
  • parameters
  • The aim of this study was to assess the relationship between the cytomorphological characteristics of nucleoli and detailed clinicopathological parameters of melanoma patients. (biomedcentral.com)
  • Separation
  • This new method consists of sonicating cells in an isotonic medium containing MgSO(4), spermidine, and spermine, followed by separation of nucleoli through a Percoll density gradient. (ac.be)
  • slides
  • Three characteristics of nucleoli - presence, size and number - of cancer cells were assessed in H&E-stained slides of 96 formalin-fixed paraffin-embedded primary cutaneous melanoma tissue specimens. (biomedcentral.com)
  • biology
  • We are now accepting submissions for our upcoming special issue on 'Reconstituting cell biology', guest edited by Manuel Théry. (biologists.org)
  • Recently, scientists from the Max Planck Institute for Biology of Ageing and the University of Cologne, found that the size of the nucleolus plays an important role in protecting cells against infection. (scitechdaily.com)
  • Trends in Cell Biology (2000) 10: 189-196. (biologyreference.com)
  • Current Opinions in Cell Biology (2000) 12: 372-377. (biologyreference.com)
  • cellular
  • Cell fractionation is both a way of reducing complexity and a way to target a specific cellular compartment. (springer.com)
  • specific
  • In Kaplan-Meier analysis, increased prevalence and amount, but not size of nucleoli, were connected with shorter cancer-specific and disease-free survival. (biomedcentral.com)
  • dynamics
  • In spite of extensive and detailed analyses in the steps and mechanisms regarding the syntheses and assembly of the ribosome, little is known as to the spatial and temporal dynamics of these processes in living cells. (rupress.org)
  • Moreover
  • Moreover, microsatellitosis was strongly correlated with the presence of macronucleoli and polynucleolization (presence of two or more nucleoli). (biomedcentral.com)