Cell myosin. Medical search. Frequent questions

The measured FRET efficiencies were intermediate between those observed when the donor was attached to the cardiac myosin regulatory light chain in the thick filaments and troponin T in the thin filaments. (kcl.ac.uk)
No thick filaments of myosin were found regardless of preparative procedures, which included fixation at rest and in contraction, glycerine extraction, and storage at low pH prior to fixation. (rupress.org)
Absence of thick myosin filaments and presence of what appear to be myosin molecules is in accord with conclusions based on X-ray diffraction (3, 12) and birefringence data (4) from living smooth muscles at rest and in contraction. (rupress.org)
Explanations are provided for appearances thought by others (6, 20, 21) to represent thick myosin filaments. (rupress.org)
The motor domain at the N-terminus of the myosin heavy chain binds actin filaments in an ATP-dependent manner. (elifesciences.org)
The C-terminal tail of the myosin heavy chain associates with the tails of other myosin heavy chains and promotes the assembly into bipolar filaments. (elifesciences.org)
The bipolar myosin filaments promote the sliding of antiparallel actin filaments relative to one another resulting in contraction of an actin network. (elifesciences.org)
The rational for the different myosin fragments lies in the different biochemical properties: Full-length myosin forms filaments, sediments at high speed and can be used in the in vitro motility assay. (elifesciences.org)
Attachment of Myosin-X to filaments of a molecule called actin which forms a sort of conveying belt within filopodia, is necessary for the movement of Myosin-X complexes towards the cell centre but this movement is strongly regulated by the part of Myosin-X which does not attach to actin but instead binds other molecules. (europa.eu)
The interactions of bipolar myosin II filaments with actin arrays are a predominate means of generating forces in numerous physiological processes including muscle contraction and cell migration. (aps.org)
Mutations in the MYH7 gene lead to the production of an altered cardiac β-myosin heavy chain protein, which is thought to be less able to form thick filaments. (medlineplus.gov)
PubMed]Murata-Hori M, Fukuta Y, Ueda K, Iwasaki T, Hosoya H. HeLa Squat kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells. (opioid-receptors.com)
Each sarcomere consists of thick filaments of myosin, shown in purple, and thin filaments of actin, shown in orange. (pearson.com)
A sarcomere contracts when its actin filaments slide past its myosin filaments. (pearson.com)
Contraction shortens the sarcomere but does not change the length of the actin or myosin filaments. (pearson.com)
The myosin filaments have heads that bind and pull actin repeatedly, shortening the sarcomere. (pearson.com)
Here, we have imaged the dynamics of green fluorescent protein-tagged myosin filaments, microtubules, and Kinesin-6 ( which carries activators of Rho guanosine triphosphatase) at the cell cortex using total internal reflection fluorescence microscopy in flattened Drosophila S2 cells. (dundee.ac.uk)
The pattern of Kinesin-6 localization closely resembles where new myosin filaments appear at the cortex by de novo assembly. (dundee.ac.uk)
While accumulating at the equator, myosin filaments disappear from the poles of the cell, a process that also requires Kinesin-6 as well as possibly other signals that emanate from the elongating spindle. (dundee.ac.uk)
The plus ends of the actin filaments are located at the tip of the microvillus and are capped, possibly by capZ proteins, [2] while the minus ends are anchored in the terminal web composed of a complicated set of proteins including spectrin and myosin II. (wikipedia.org)
Actin filaments, present in the cytosol , are most abundant near the cell surface. (wikipedia.org)
Outside the cell myosin can be used to move protein filaments, composed of actin, along artificial paths, which direct the filaments' movements. (lu.se)
[ 3 ] Nodal cells have less well-organized actin and myosin filaments and a poorly developed sarcoplasmic reticulum. (medscape.com)

Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
Work done using smooth muscle myosin and mammalian non-muscle myosin have demonstrated that phosphorylation of the RLC at conserved Serine and Threonine sites ( Figure 1B , Serine-19 and Threonine-18) activates myosin motor activity, enhances the affinity of myosin for actin, and promotes myosin filament assembly ( Heissler and Sellers, 2016 ). (elifesciences.org)
However, it has not been biochemically demonstrated that Drosophila myosin motor activity and filament assembly is regulated by RLC phosphorylation or whether the extent of activation is similar to that of mammalian systems. (elifesciences.org)
Specifically, ROCK acts as a substrate for myosin light chain, facilitating the phosphorylation and dephosphorylation of the MYL9 gene. (cusabio.com)
Numerous studies have shown that MYL9 regulates ATPase activity and myosin contraction through phosphorylation and dephosphorylation. (cusabio.com)
PubMed]Kawano Y, Fukata Y, Oshiro D, Amano Meters, Nakamura Testosterone levels, Ito Meters, Matsumura Y, Inagaki Meters, Kaibuchi T. Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo. (opioid-receptors.com)
Apoptotic membrane blebbing is usually regulated by myosin light chain phosphorylation. (opioid-receptors.com)
These results indicate that P-light chain phosphorylation affects actin-myosin interactions in cardiac and skeletal muscles at submaximal levels of Ca 2+ activation. (elsevierpure.com)
Sweeney, HL & Stull, JT 1986, ' Phosphorylation of myosin in permeabilized mammalian cardiac and skeletal muscle cells ', American Journal of Physiology - Cell Physiology , vol. 250, no. 4 (19/4), pp. (elsevierpure.com)
Quantitation of the distribution and flux of a mutant myosin-II that is defective in phosphorylation-dependent thick filament disassembly confirms that heavy chain phosphorylation regulates normal recruitment to the furrow cortex. (biomedcentral.com)
The analysis indicates that myosin-II flux through the cleavage furrow cortex is regulated by thick filament phosphorylation. (biomedcentral.com)
Signalling downstream of the small GTPase Rho increases contractility through Rho-kinase (ROCK)-mediated regulation of myosin-II light chain (MLC2) phosphorylation. (ed.ac.uk)
MRCK (myotonic dystrophy kinase-related Cdc42-binding kinase) cooperates with ROCK in the maintenance of elongated morphology and invasion and either MRCK or ROCK is sufficient for MLC2 phosphorylation, through the inhibitory phosphorylation of myosin phosphatase. (ed.ac.uk)
CaMKII-mediated phosphorylation of the myosin motor Myo1c is required for insulin-stimulated GLUT4 translocation in adipocytes. (bvsalud.org)
Myo1c ATPase activity was increased after CaMKII phosphorylation in vitro and after insulin stimulation of CHO/IR/IRS-1 cells . (bvsalud.org)

We identified 231 proteins released from actomyosin contraction-dependent blebs and apoptotic bodies by adapted SILAC (stable isotope labeling with amino acids in cell culture) combined with mass spectrometry analysis. (nature.com)
Cardiac myosin binding protein-C (cMyBP-C) is one of the proteins that make up the contractile apparatus of cardiomyocytes. (scirp.org)
Myosins ( / ˈ m aɪ ə s ᵻ n , - oʊ - / ) comprise a family of ATP -dependent motor proteins and are best known for their role in muscle contraction and their involvement in a wide range of other motility processes in eukaryotes . (wn.com)
Myosin-X is a member of a family of proteins called Myosins known to shuttle other molecules within the cell and thus allow their localization at the place where they are required. (europa.eu)
Myosins are conserved proteins that serve as actin-dependent molecular motors, playing important roles in the cytoskeleton. (cusabio.com)
Using a broad spectrum anti-acetyl antibody, the researchers determined that one of the acetylated proteins is myosin. (cytoskeleton.com)
Recently, Foster et al 10 reported that in an initial acetylome of porcine heart proteins, 240 proteins were modified on 994 lysine residues with myosin acetylated on many different lysine amino acids. (cytoskeleton.com)
Furthermore, we observed in these knockdown cells an upregulation of constitutive secretion of alkaline phosphatase, implying that both proteins act as negative regulators of secretory traffic at the Golgi complex. (ox.ac.uk)
Researchers had hoped to find that matters would be clarified by myosin proteins derived from the DNA sequences of different single-celled eukaryotes, such as flagellated protozoa (protozoa with a whip-like tail), amoeboid protozoa, and algae. (icr.org)

Nonmuscle myosin II (NM-II) is an important motor protein involved in cell migration. (nih.gov)
Dying cells have been defined as apoptotic by distinguishing features, including cell contraction, nuclear fragmentation, blebbing, apoptotic body formation and maintenance of intact cellular membranes to prevent massive protein release and consequent inflammation. (nature.com)
Blebbing, apoptotic body formation and protein release during early apoptosis are dependent on ROCK and myosin ATPase activity to drive actomyosin contraction. (nature.com)
Cardiac myosin binding protein-C (cMyBP-C) is a thick filament-associated regulatory protein frequently found mutated in patients suffering from hypertrophic cardiomyopathy (HCM). (kcl.ac.uk)
Myosin-2 (myosin heavy chain 2) is a protein that in humans is encoded by the MYH2 gene. (wikipedia.org)
Schlossarek, S., Mearini, G. and Carrier, L. (2011) Cardiac myosin-binding protein c in hypertrophic cardiomyopathy: Mechanisms and therapeutic opportunities. (scirp.org)
Thus, although myosin was originally thought to be restricted to muscle cells (hence myo- (s) + -in ), there is no single "myosin" but rather a huge superfamily of genes whose protein products share the basic properties of actin binding, ATP hydrolysis (ATPase enzyme activity), and force transduction. (wn.com)
This condition is characterized by the formation of protein clumps, which contain a protein called myosin, within certain muscle fibers. (medlineplus.gov)
The MYH7 gene provides instructions for making a protein known as the cardiac beta (β)-myosin heavy chain. (medlineplus.gov)
Additionally, several other important molecules such as calmodulin-dependent protein kinase II, ILK , PKA, ZIPK , PKC, and the inhibitory molecule CP17 of myosin light chain phosphatase MLCP are also involved [12-14] . (cusabio.com)
The protein product of MYH16 is one of the heavy chain myosins, a kind of protein that works with actin to enable muscle fibers to contract. (johnhawks.net)
The protein is denoted as MyHC-M, for myosin heavy chain-masticatory. (johnhawks.net)
INTRODUCTION Apoptotic cell death is usually an indispensable process for embryonic development and maintenance of tissue homeostasis in multicellular organisms (Jacobson and for 10 min at 4?C. The cell lysate was incubated with anti-V5 antibody bound to Dynabeads Protein G (Invitrogen) under constant rotation for 1 h. (opioid-receptors.com)
Indirect immunofluorescence Indirect immunofluorescence was carried out as described previously (Murata-Hori cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. (opioid-receptors.com)
In fact, myosin is the most highly acetylated protein found to date with 49 acetylated lysine residues 10 . (cytoskeleton.com)
We carried out a yeast two-hybrid screen and identified TRAF6-binding protein (T6BP) and nuclear dot protein 52 (NDP52) as myosin VI binding partners. (ox.ac.uk)
Myosin VI interaction with T6BP and NDP52 was confirmed in vitro and in vivo and the binding sites on each protein were accurately mapped. (ox.ac.uk)
In the enterocyte microvillus, the structural core is attached to the plasma membrane along its length by lateral arms made of myosin 1a and Ca 2+ binding protein calmodulin . (wikipedia.org)
In tem1Δ cells expressing a Cnm67-Tem1 fusion protein, Cdc15-3HA (red) loads prematurely onto spindle pole bodies during metaphase as determined by spindle morphology (tubulin, green) and nuclear mor. (cellimagelibrary.org)
Molecular motors are important features of eukaryotic cells that are formed by a variety of protein types. (icr.org)
In several instances, such as rheumatoid arthritis, multiple sclerosis, and myocarditis, the autoimmune disease can be induced experimentally by administering self-antigen in the presence of adjuvant (col- lagen, myelin basic protein, and cardiac myosin, respec- tively) (3). (cdc.gov)
actin is a ubiquitous contractile protein that can be found in non-muscle cells. (medscape.com)

These data demonstrate an essential role of myosin Va in cancer cell migration and metastasis, and suggest a novel target for Snail in its regulation of cancer progression. (elsevierpure.com)
Recent research by the Randall Centre for Cell and Molecular Biophysics provides evidence for the role of myosin-based regulation in the potentiation of heart. (kcl.ac.uk)
[ 4 ] The exact role of myosin Va in Griscelli syndrome has yet to been defined further. (medscape.com)
The role of Myosin Va in the neuritogenesis of dorsal root ganglia TrkA-positive neurons. (usp.br)

This gene encodes a member of the myosin superfamily. (nih.gov)

LIMCH1 interacted with NM-IIA, but not NM-IIB, independent of the inhibition of myosin ATPase activity with blebbistatin. (nih.gov)
Phosphorylating MYL9 improves the interaction between myosin and actin, as well as enhances ATPase activity in the myosin light chain head. (cusabio.com)

They explained it by 1) convergence (the sudden and simultaneous appearance of a gene with no evolutionary patterns in different taxa), 2) lineage-specific expansions (different myosin gene complements found in different creatures), and 3) gene losses (missing genes that evolutionists thought should have been there). (icr.org)
Some myofibers which appear to have split contain different myosin isoforms whereas others are homogeneous. (cdc.gov)

Karam S, Raboisson MJ, Ducreux C, Chalabreysse L, Millat G, Bozio A, Bouvagnet P: A de novo mutation of the beta cardiac myosin heavy chain gene in an infantile restrictive cardiomyopathy. (karger.com)
Cardiac β-myosin heavy chain is the major component of the thick filament in muscle cell structures called sarcomeres . (medlineplus.gov)
The heart achieves the coordinated contraction of the atrial and ventricular chambers due to the precise timing of the cardiac conduction system (CCS), a specialized complex and heterogeneous network of cells that initiate and allow propagation of action potentials through the heart. (medscape.com)
The normal cardiac impulse of the vertebrate heart originates in the pacemaker cells of the sinoatrial node, located in the right atrium. (medscape.com)

Depletion of LIMCH1 attenuated myosin regulatory light chain (MRLC) diphosphorylation in HeLa cells, which was restored by reexpression of small interfering RNA-resistant LIMCH1. (nih.gov)
Myosin is a hexamer composed of two myosin heavy chains, two regulatory light chains (RLCs), and two essential light chains (ELCs) ( Figure 1A ). (elifesciences.org)
MYL9, the regulatory light chain of myosin, is essential for cell movement by activating myosin motility. (cusabio.com)
It encodes the 20KD myosin regulatory light chain. (cusabio.com)
The neck contains an alpha helix entwined by two regulatory light chains that bind to myosin light chains and calmodulin. (cusabio.com)
PubMed]Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A, Hosoya H. Squat kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. (opioid-receptors.com)
Primary endothelial cells from transgenic mouse expressing myosin heavy chain 2A fused to mCherry (red), transfected with GFP-myosin (green) regulatory light chain expression vector and demonstrate th. (cellimagelibrary.org)

Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. (elifesciences.org)

Cell surface sensors on immune cells that ordinarily recognize pathogen-associated molecular patterns produced by infectious agents may also interact with DAMPs, leading to the activation of signaling pathways resulting in a nonpathogen-induced 'sterile' inflammatory response. (nature.com)
Structures with the characteristics of molecular myosin were identified by electron microscopy in tissue sections of vertebrate smooth muscle. (rupress.org)
Molecular biology of the cell. (vanderbilt.edu)
We have found that two actin-dependent molecular motors, class 1 myosins myosin 1e and myosin 1f, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. (biorxiv.org)
Identifying the mechanical properties of cells that allow them to undergo shape changes and elucidating the molecular mechanisms that cells use to generate the mechanical forces remains the ultimate challenge of understanding cellular morphogenesis. (biomedcentral.com)
Here, we report that an actin-dependent molecular motor, unconventional myosin Va, is involved in this process and implicated in cancer metastasis. (elsevierpure.com)
In 2018 he was awarded a Sir Henry Dale Fellowship (Wellcome Trust/Royal Society) to develop his laboratory at the Randall Centre for Cell and Molecular Biophysics. (kcl.ac.uk)
One group of molecular motors is called the myosins, which have recently been studied in everything from one-celled eukaryotes to humans. (icr.org)
Molecular motors are large molecules that carry out mechanical tasks in living cells. (lu.se)

The defect in the myosin motor activity in these mutants is evident in developing Drosophila embryos where tissue recoil following laser ablation is decreased compared to wild-type tissue. (elifesciences.org)
In Griscelli syndrome type 1, a defect in the myosin Va gene leads to the pigment dilution and neurological sequelae. (medscape.com)

Numerous studies have demonstrated that MYL9, as a crucial member of the myosin family, participates in various organismal functions regulated by diverse factors. (cusabio.com)

In addition to maintenance of membrane integrity, apoptotic cells can be discriminated from viable counterparts based on several morphological hallmarks, including cell contraction, nuclear condensation and fragmentation, and actomyosin contraction-dependent membrane blebbing and apoptotic body formation. (nature.com)
Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos. (elifesciences.org)
Intermicrovillous space increases with contractile activity of myosin II and tropomyosin , and decreases when contraction ceases. (wikipedia.org)
They also suggest that oIAPP induces contraction of these mural cells and that pramlintide can reverse such contraction. (lu.se)
The tips of the Purkinje fibers are electrically coupled to muscle cells and the working myocytes are longitudinally connected via gap junctions, thereby initiating a coordinated, efficient contraction of the ventricles. (medscape.com)

Abnormal expression of MYL9, which is associated with cell motility. (cusabio.com)
Our data show that a Cdc42-MRCK signal mediates myosin-dependent cell motility and highlight convergence between Rho and Cdc42 signalling. (ed.ac.uk)
This continues for 10 minutes after division before the daughter cells revert to normal random motility, indicating that this is a tightly regulated process. (biologists.com)
Myosin VI has been implicated in many cellular processes including endocytosis, secretion, membrane ruffling and cell motility. (ox.ac.uk)

Bridgman had previously noticed that neuronal growth cones contain high levels of myosin II. (rupress.org)
In addition, the levels of myosin Va in cancer cell lines are positively correlated with the expression of Snail, a transcriptional repressor that triggers epithelial-mesenchymal transition. (elsevierpure.com)
Repression or overexpression of Snail in cancer cells caused reduced or elevated levels of myosin Va, respectively. (elsevierpure.com)

it should not be confused with the conventional skeletal muscle myosin-1 (MYH1). (nih.gov)
In 2014 he was awarded a King's Prize Fellowship to study a novel form of regulation of contractility in skeletal muscle based on the myosin filament. (kcl.ac.uk)
Injury to skeletal muscle has been associated with proliferation and migration of cells into regions with damaged myofibers. (cdc.gov)

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. (joplink.net)
One dramatic example of a process in which a cell reshapes itself is during the mechanical separation of a mother cell into two daughter cells during cytokinesis. (biomedcentral.com)
One appeal of studying cytokinesis as a model for cellular morphogenesis is its relatively simple geometry during normal mitotic cell divisions, which produce equal sized daughter cells. (biomedcentral.com)
We demonstrate that SCAR activity is essential to drive myosin-II-independent cytokinesis, and stabilises the furrow, ensuring symmetrical division. (biologists.com)
These results suggest models for how Kinesin-6 might define the position of cortical myosin during cytokinesis. (dundee.ac.uk)

Collectively, these observations suggest that guidance cues cause axon retraction through the coordinated activation of myosin II and the formation of intra-axonal F-actin bundles for myosin-II-based force generation. (biologists.com)
This activation triggers the activation of myosin light chain myosin. (cusabio.com)

Our representation of myosin motors can be used to understand how their mechanical and biochemical properties influence their observed behavior in a variety of in vitro and in vivo contexts. (aps.org)
Schematic representation of myosin structure and the positions of published acetylation sites. (cytoskeleton.com)

The subsequent activation of focal adhesion kinases might activate or recruit myosin II. (rupress.org)
Myosin placement in relation to adhesion sites might pull neurites toward more LN1 and away from unwanted substrates. (rupress.org)
Using primary macrophages lacking both myosin 1e and myosin 1f, we found that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. (biorxiv.org)
This work identifies a novel role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a model for a membrane-tension based feedback mechanism for phagocytic cup closure. (biorxiv.org)
Caspase-mediated cleavage of focal adhesion kinase disassembly and pp125FAK of focal adhesions in individual endothelial cell apoptosis. (opioid-receptors.com)
The best understood of these involves myosin-II-dependent constriction around the cell equator, but both Dictyostelium and mammalian cells also use a parallel, adhesion-dependent mechanism to generate furrows. (biologists.com)
EB1-null cells also exhibit decreased adhesion during mitosis. (biologists.com)
Cell migration, which involves acto-myosin dynamics, cell adhesion, membrane trafficking and signal transduction, is a prerequisite for cancer cell metastasis. (elsevierpure.com)
T6BP and NDP52 are myosin VI binding partners with potential roles in cytokine signalling and cell adhesion. (ox.ac.uk)
Although the SKICH domain in T6BP and NDP52 does not mediate recruitment into membrane ruffles, loss of T6BP and NDP52 in RNAi knockdown cells results in reduced membrane ruffling activity and increased stress fibre and focal adhesion formation. (ox.ac.uk)
Thus myosin VI-T6BP interactions may link membrane trafficking pathways with cell adhesion and cytokine-dependent cell signalling. (ox.ac.uk)

Insights provided by comparisons of kindreds with distinct and identical beta-myosin heavy chain gene mutations. (karger.com)
Van Driest SL, Jaeger MA, Ommen SR, Will ML, Gersh BJ, Tajik AJ, Ackerman MJ: Comprehensive analysis of the beta-myosin heavy chain gene in 389 unrelated patients with hypertrophic cardiomyopathy. (karger.com)
Mutations in the MYH7 gene cause myosin storage myopathy. (medlineplus.gov)
which means one copy of the altered gene in each cell is sufficient to cause the disorder. (medlineplus.gov)
Griscelli syndrome type 2 is caused by a defect in the RAB27A gene, which affects a melanosome-anchoring complex in melanocytes, affecting release of cytolytic granules from T cells and natural killer cells. (medscape.com)
We are using gene editing techniques of immune cells to understand the relation of cell transformation and specific genetic mutations identified in primary immunodeficiency patients and lymphoma patients. (ki.se)
So, how did the authors explain the incredible complexity found across the spectrum of life in myosin gene content that had no clear evolutionary patterns? (icr.org)
None of these ideas actually explain why there is no evolutionary pattern of simple-to-complex in myosin gene content across the spectrum of life. (icr.org)

abstract = "Actomyosin contractility is a mechanism by which cells exert locomotory force against their environment. (ed.ac.uk)

The light chains bind to the central neck domain of the myosin heavy chain and have structural and regulatory functions ( Heissler and Sellers, 2014 ). (elifesciences.org)
The top panel shows the myosin hexamer composed of two myosin heavy chains (green), two ELCs (light blue) and two RLCs (gray). (elifesciences.org)

Virtually all eukaryotic cells contain myosin isoforms . (wn.com)
Some isoforms have specialized functions in certain cell types (such as muscle), while other isoforms are ubiquitous . (wn.com)
The present study attempted to provide information regarding non-muscle myosin II (MII) isoforms immunoreactivity in patients with head and neck squamous cell carcinoma (HNSCC) and analysis of the patients? (uv.es)
PMC free of charge content] [PubMed]Mabuchi T, Gong BJ, Langsetmo T, Ito Meters, Nakano T, Tao T. Isoforms of the small non-catalytic subunit of easy muscle myosin light chain phosphatase. (opioid-receptors.com)

The dephosphorylation activity of Rabbit Polyclonal to HUNK myosin phosphatase immunoprecipitated from the apoptotic cells was lower than that from the nonapoptotic control cells. (opioid-receptors.com)
Identification of the endogenous easy muscle myosin phosphatase-associated kinase. (opioid-receptors.com)
PMC free article] [PubMed]Mizuno T, Tsutsui K, Nishida Y. myosin phosphatase and its role in dorsal closure. (opioid-receptors.com)

Here, combining the biochemical analysis of enzymatic and motile properties for purified myosin mutants with in vivo measurements of apical constriction for the same mutants, we show that in vivo constriction rate scales with myosin motor activity. (elifesciences.org)

J Cell Biol (2005) 169 (5): 703. (rupress.org)
J Cell Biol (1970) 44 (1): 52-61. (rupress.org)
L Cell Biol. (opioid-receptors.com)
Nat Cell Biol. (opioid-receptors.com)
J Cell Biol. (opioid-receptors.com)
J Cell Biol (2008) 181 (1): 15-18. (silverchair.com)

Armel TZ, Leinwand LA. Mutations in the beta-myosin rod cause myosin storage myopathy via multiple mechanisms. (medlineplus.gov)

The unconventional myosin Myo1c has been implicated in insulin -regulated GLUT4 translocation to the plasma membrane in adipocytes . (bvsalud.org)

In addition, LIMCH1-depleted HeLa cells exhibited a decrease in the number of actin stress fibers and focal adhesions, leading to enhanced cell migration. (nih.gov)
Collectively, our data suggest that LIMCH1 plays a positive role in regulation of NM-II activity through effects on MRLC during cell migration. (nih.gov)
It has been shown that MYL9 promotes cancer cell migration and is implicated in tumor development and progression. (cusabio.com)
Suppressing the expression of myosin Va by lentivirus-based RNA interference in highly metastatic cancer cells impeded their migration and metastasis capabilities both in vitro and in vivo. (elsevierpure.com)
Is the Subject Area "Cell migration" applicable to this article? (plos.org)
Microvilli are also of importance on the cell surface of white blood cells , as they aid in the migration of white blood cells. (wikipedia.org)

This interaction enables the recruitment of T cells to the inflamed lung, which is significant in understanding immune responses [1] . (cusabio.com)

Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. (biorxiv.org)
The myosin-II in the furrow cortex, however, represents only 10% of the total cellular myosin-II. (biomedcentral.com)
Thus, microvilli not only increase the cellular surface area for absorption, they also increase the number of digestive enzymes that can be present on the cell surface. (wikipedia.org)

Myosin-1A targets to microvilli using multiple membrane binding motifs in the tail homology 1 (TH1) domain. (nih.gov)
As mentioned, microvilli are formed as cell extensions from the plasma membrane surface. (wikipedia.org)

Following the discovery by Pollard and Korn (1973) of enzymes with myosin-like function in Acanthamoeba castellanii , a large number of divergent myosin genes have been discovered throughout eukaryotes. (wn.com)
The diverse human myosins family consists of 12 classes (I-XII) with 40 identified myosin genes. (cusabio.com)
1 Instead of finding a pattern of evolving myosin 'motor' genes (simple to complex) as life seemingly became more advanced, they found that the highest numbers of different types of myosin genes were found in single-celled eukaryotes. (icr.org)
The authors stated, 'The number of myosin genes varies markedly between lineages [types of eukaryotes],' and 'holozoan genomes, as well as some amoebozoans and heterokonts, have the highest numbers of myosins of all eukaryotes. (icr.org)
In particular, the haptophyte Emiliania huxleyi has the highest number of myosin genes (53), followed by the ichthyosporean Pirum gemmata (43), the filasterean M. vibrans (39), and the metazoan Homo sapiens (38). (icr.org)
Specifically, the ideas of convergent evolution and lineage-specific expansions are nothing more than fancy terms for the fact that these different types of myosin genes appeared suddenly in unrelated creatures at the same time. (icr.org)

I report that semaphorin 3A activates myosin II in growth cones and axons. (biologists.com)

Tajsharghi H, Thornell LE, Lindberg C, Lindvall B, Henriksson KG, Oldfors A. Myosin storage myopathy associated with a heterozygous missense mutation in MYH7. (medlineplus.gov)

A focus is the role of nuclear localization of WASp family members and actin in immune cells ( Kuznetov et al, Genome Med 2017 ). (ki.se)

Inhibition of Ced-3/ICE-related Acacetin IC50 proteases does not prevent cell death induced by oncogenes, DNA damage, or the Bcl-2 homologue Bak. (opioid-receptors.com)

Nature Cell Biology, 4, E127-E130. (scirp.org)
Nature Cell Biology , 7 (3), 255-61. (ed.ac.uk)
Journal of Cell Biology , 186 (5), 727-738. (dundee.ac.uk)

A ) Domain organization of the myosin heavy chain and myosin fragments used to study the biochemical properties of myosin. (elifesciences.org)

Mutations in myosin heavy chain 7 (MYH7) commonly cause cardiomyopathy. (karger.com)
We have a particular focus on how inborn mutations in actin regulators affect immune cell-cell communication, trafficking, and maintenance of genomic stability. (ki.se)

MYL9: a New Member of Myosin Motors, a Key Driver of Cancer Cell Dynamic Regulation? (cusabio.com)
These results suggest that down-regulation of MYPT1 may play a role in promoting hyperphosphorylation of myosin II by inhibiting the dephosphorylation of myosin II during apoptosis. (opioid-receptors.com)

The three-dimensional structures of the truncated myosin head from Dictyostelium discoideum myosin II complexed with dinitrophenylaminoethyl-, dinitrophenylaminopropyl-, o-nitrophenylaminoethyl-, m-nitrophenylaminoethyl-, p-nitrophenylaminoethyl-, and o-nitrophenyl-N-methyl-aminoethyl-diphosphate.beryllium fluoride have been determined to better than 2.3-A resolution. (rcsb.org)

The peak concentration of GFP-myosin-II in the furrow cortex is 1.8-fold higher than in the polar cortex and 2.0-fold higher than in the cytoplasm. (biomedcentral.com)

Using pharmacological and genetic perturbations in cultured intestinal epithelial cells, we found that NM2C controls the length of growing microvilli by regulating actin turnover in a manner that requires a fully active motor domain. (vanderbilt.edu)

In cells, F-actin assumes specific types of organization depending on its functions. (biologists.com)
Functionally, myosin motors have emerged as powerful players that drive cytoplasmic streaming, actin organization, and cell expansion ( Figure 1 ) [3-6] . (cusabio.com)

The structure and function of myosin is strongly conserved across species, to the extent that rabbit muscle myosin II will bind to actin from an amoeba . (wn.com)
Furthermore, Snail can bind to an E-box of the myosin Va promoter and induce its activity, which indicates that Snail might act as a transcriptional activator. (elsevierpure.com)

The cell may, therefore, regulate the amount of myosin-II sent to the furrow cortex in accordance with the amount needed there. (biomedcentral.com)
Further, the amount of myosin-II observed in the furrow cortex is in close agreement with the amount predicted to be required from a simple theoretical analysis. (biomedcentral.com)

On polyornithine, both myosin II and focal complexes are randomly distributed. (rupress.org)
On LN1, however, myosin IIB concentrated in the transitional domain of the growth cone-intermingled with or just behind the new front of focal complexes. (rupress.org)

Myosin II interacts with F-actin to generate contractile forces that result in axon retraction. (biologists.com)
PubMed]Iwasaki T, Murata-Hori M, Ishitobi T, Hosoya L. Diphosphorylated MRLC is certainly needed for firm of tension fibres in interphase cells and the contractile band in dividing cells. (opioid-receptors.com)
Here we report the first quantitative analysis of the distribution and flux of myosin-II, an essential element of the contractile ring. (biomedcentral.com)
In their model, the minimal contractile force required for stabilizing each of these intermediate shapes is proportional to the global steady state stiffness of the cell and is dependent on the extent of furrow ingression. (biomedcentral.com)
In the latter, IAPP is often found in vessels, where it is highly toxic for pericytes, mural cells that have contractile properties and regulate capillary blood flow. (lu.se)

Cell division requires the tight coordination of multiple cytoskeletal pathways. (biologists.com)

Though the length and composition of microvilli is consistent within a certain group of homogenous cells, it can differ slightly in a different part of the same organism. (wikipedia.org)
The fictional LECA creature represents the final stage of a transition between a bacterial-archaeal prokaryote (the smallest and simplest organism) and a one-celled eukaryote (a cell with a nucleus and other organelles). (icr.org)

MYL9 exerts regulatory functions through two major systems: the Rho-kinase ( ROCK ) system and the myosin light chain kinase ( MLCK ) system. (cusabio.com)

Anatomy14

Organisms8

Chemicals and Drugs43

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes13

Information Science2

Filaments23

Phosphorylation15

Proteins9

Protein22

Role of myosin4

Superfamily1

ATPase2

Different myosin2

Cardiac4

Regulatory light7

Cytoskeleton1

Molecular9

Defect in the myosin2

Member of the myosin1

Contraction5

Motility4

Levels of myosin3

Skeletal3

Cytokinesis5

Activation of myosin2

Representation of myosin2

Adhesion11

Gene8

Abstract1

Chains2

Isoforms4

Phosphatase3

Constriction1

Biol6

Beta-myosin1

Unconventional myosin1

Migration6

Interaction1

Cellular3

Membrane2

Genes6

Activates1

Missense mutation1

Localization1

Inhibition1

Biology3

Fragments1

Mutations2

Regulation2

Dictyostelium1

Cytoplasm1

Growing microvilli1

Organization2

Bind2

Cortex2

Focal2

Contractile5

Pathways1

Organism2

MLCK1