• Exp Cell Res, 2022 Feb 1. (nih.gov)
  • 2016. Profilin-dependent nucleation and assembly of actin filaments controls cell elongation in Arabidopsis. (purdue.edu)
  • J Cell Sci, 2016 Apr 15. (nih.gov)
  • Mol Med Rep . 2016 May. (medscape.com)
  • Mol Biol Cell, 2018 Dec 1. (nih.gov)
  • doi: 10.1016/j.cell.2016.04.059. (nih.gov)
  • Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro. (bvsalud.org)
  • The second approach is to isolate actin-associated proteins using molecular and biochemical techniques, to characterize their function in vitro, and to use these as probes to dissect cytoskeletal function in living plant cells. (purdue.edu)
  • Ser-175 in vitro and in Caki-2 cells. (researchreportone.com)
  • Therefore, many proteins that were implicated by proteomic analysis as getting shed by MMP-14, determined by increased levels inside the conditioned medium upon expression of MMP-14 in MDA-MB-231 cells and decreased levels in the presence of a MMPI, have been biochemically validated as substrates of MMP-14 in vitro. (translateinthetownships.com)
  • In 2015, Dr. Qiang returned to Drexel University, where he now leads a research laboratory that focuses on using human induced pluripotent stem cell (hiPSC) based technologies to develop in vitro and ex vivo cellular and tissue models. (drexel.edu)
  • Because alcohol blocks airway epithelial cell release of IL-6 in vitro , we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. (cdc.gov)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-κB activation. (wikipedia.org)
  • This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. (wikipedia.org)
  • The majority of clear cell RCC tumors have lower than normal levels of a protein called von Hippel-Lindau, which leads to higher levels of MET, AXL and VEGF. (businesswire.com)
  • The FLI protein controls the growth and development of some cell types by regulating the transcription of certain genes. (medlineplus.gov)
  • Recent studies indicate that nutrient fluctuations and insulin resistance increase proinsulin synthesis in β cells beyond the capacity for folding of nascent polypeptides within the endoplasmic reticulum (ER) lumen, thereby disrupting ER homeostasis and triggering the unfolded protein response (UPR). (jci.org)
  • These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
  • Protein kinases are key regulators of signal transduction pathways that mediate cell growth, proliferation, and motility. (drexel.edu)
  • The work in Dr. Strochlic's lab is broadly centered on understanding certain protein kinase-mediated cell signaling pathways in the context of normal development and tumorigenesis. (drexel.edu)
  • The lab uses a combination of genetic, biochemical, and cell biological approaches in both Drosophila melanogaster and mammalian cells to investigate the biology of several different protein kinases. (drexel.edu)
  • Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway. (uib.no)
  • 2008). Abolition of stress-induced protein synthesis sensitizes leukemia cells to anthracycline-induced death. (uib.no)
  • Cell membrane accumulation of membrane or membrane-associated substrate candidatesaMMPI/vehicle Protein Medium Ratio No. of peptides Membrane Ratio No. of peptidesranging from 14.eight to 7.four kDa (Fig. 3D). (translateinthetownships.com)
  • Prof. Lederkremer's lab has been interested in the mechanisms of protein folding and trafficking in the early secretory pathway of mammalian cells. (tau.ac.il)
  • His group has found that interference with ERAD and upregulation of the UPR are a main mechanism of cell damage in a neurodegenerative disorder, Huntington's disease, and that this occurs prior to the appearance of the characteristic large protein inclusions that occur in this disease. (tau.ac.il)
  • Repression of Pumilio Protein Expression by Rbfox1 Promotes Germ Cell Differentiation. (tcd.ie)
  • BLM encodes 1417 amino acids that code for a protein in the nuclear matrix of growing cells, which is a member of the RecQ family of helicases. (medscape.com)
  • Image-derived models of cell organization changes during differentiation and drug treatments. (cmu.edu)
  • We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
  • Evidence is accumulating that small G proteins are involved in the regulation of cell growth and differentiation. (nii.ac.jp)
  • We have found that the levels of smg p21 mRNA increase in hematopoietic leukemiae cells after their differentiation into hematopoietic cells. (nii.ac.jp)
  • We have also found that smg p25A is mainly detected inneural tissues by use of its specific monoclonal antibody, and that the levels of smg p25A mRNA increase in PC12 cells after their differentiation into neuron-like cells. (nii.ac.jp)
  • Transcription factor Sp2 promotes TGFB-mediated interstitial cell osteogenic differentiation in bicuspid aortic valves through a SMAD-dependent pathway. (nih.gov)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • 2000. A metabolite of methoxychlor, 2,2-bis( p hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat leydig cells through suppression of steady-state messenger ribonucleic acid levels of the cholesterol side-chain cleavage enzyme. (cdc.gov)
  • which means both copies of the gene in each cell have mutations. (medlineplus.gov)
  • We performed mutagenesis to introduce changes equivalent to 12 human CACH/VWM mutations in three subunits of the equivalent factor from yeast (Saccharomyces cerevisiae) and analyzed effects on cell growth, translation, and gene expression in response to stresses. (yeastgenome.org)
  • The flow cytometry-based Pig-a assay detects cells deficient in Glycosylphosphatidylinositol (or GPI)-anchored surface markers and provides a rapid and cost-effective enumeration of cells that are presumed to contain mutations in the endogenous X-linked Pig-a gene. (fda.gov)
  • Somatic mutations are genetic alterations in cells that increase cancer risk. (fda.gov)
  • Dr. Revollo is developing NGS methods capable of directly and efficiently identifying somatic mutations in the whole genome - in any tissue, and in any species, or any established cell culture - without the need for selecting and expanding cells that have mutations in only a few specific reporter genes. (fda.gov)
  • BML mutations thus result in defects in DNA repair and genomic instability in the somatic cells, predisposing the patients to cancer development. (medscape.com)
  • Genome Biol 2006, 7(11):120. (crossref.org)
  • [ 7 , 8 ] Sister chromatid exchanges are considered a sensitive indicator for cell genome instability, as they are thought to be the outcome of DNA double-strand breaks resulting from homologous recombination repair. (medscape.com)
  • Oppositely, expression of mutated myocilin sensitizes cells to apoptosis induced by oxidative stress. (nih.gov)
  • Ipsen remains on track to submit the regulatory dossier for cabozantinib as a treatment for first-line advanced renal cell carcinoma in Europe in the third quarter of 2017. (businesswire.com)
  • Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
  • In their absence, the bulk of the matrix proteins are mislocalized to the cytosol 5 , even though these cells still contain peroxisomal membrane structures to which peroxisomal membrane proteins (PMPs) are sorted 6 . (rug.nl)
  • Such membrane structures were assumed to be absent in pex3 and pex19 mutant cells and, consequently, it was proposed that Pex3 and Pex19 are involved in peroxisomal membrane formation 1,7 . (rug.nl)
  • Both fundamentally different models explain why cells lack peroxisomal membrane structures in the absence of Pex3 or Pex19: in model A, PMPs are instable or mislocalized to the cytosol in pex3 or pex19 cells, whereas in model B, PMPs accumulate at the ER. (rug.nl)
  • Immunolabeling revealed that anacardic acid induced marked membrane accumulation of the V-ATPase A subunit in transfected Caki-2 cells. (researchreportone.com)
  • Cells were then incubated with concanavalin A coupled to CY3 for 3 min before fixation to label the plasma membrane (1:100 dilution in PBS made up of Mg2+ and Ca2+). (researchreportone.com)
  • Some of the proteins are usually not themselves membrane proteins but are likely to become bound to the cell by means of interactions with membrane-tethered molecules like heparan sulfate proteoglycans and receptors (Fig. 1B and D) or by interaction with exosites on the stabilized inhibited mature MMP-14, a type of "substrate trap" (Fig. 1E). (translateinthetownships.com)
  • Depletion of SMC5/6 sensitizes male germ cells to DNA damage. (nih.gov)
  • In contrast to the former views, it was found that pre-peroxisomal vesicles (PPV's) exist in yeast pex3 and pex19 cells. (rug.nl)
  • The peroxisomal PTS1-Import defect of PEX1-deficient cells is independent of pexophagy in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)
  • The first, a cytological and molecular genetic analysis of cell division, makes use of a large collection of maize meiotic mutants. (purdue.edu)
  • Members of the institute have published in a wide range of peer-reviewed journals, including Nature, Molecular Cell, Angewandte Chemie, JACS, Science Advances, Proceedings of the National Academy of Sciences USA, Nucleic Acids Research, Chemical Science and many others. (le.ac.uk)
  • Ast five forms with reduce molecular massesBUTLER ET AL.MOL. (translateinthetownships.com)
  • Using synchronized cells undergoing recombination that is initiated at a specific site on a chromosome by an inducible endonuclease, we use physical monitoring techniques (Southern blots, PCR analysis) to follow the sequence of molecular events that occur in real time. (brandeis.edu)
  • Pazos F, Juan D, Izarzugaza JMG, Leon E, Valencia A: Methods in Mol Biol (Clifton, N.J.). 2008, 484: 523–535. (crossref.org)
  • Methods in Mol Biol (Clifton, N.J. (crossref.org)
  • Methods Mol Biol (Clifton, N.J.) 2012, 812: 161–174. (crossref.org)
  • Ravasz E: Methods Mol Biol (Clifton, N.J.). 2009, 541: 145–160. (crossref.org)
  • Although many methods and approaches exist, identifying cell states and their underlying topology is still a major challenge. (nature.com)
  • Methods Mol Biol;2711: 225-233, 2024. (bvsalud.org)
  • The overall result of the genomic instability in the proliferating cells is a high risk of malignancy, reduced fertility or infertility, B- and T-cell immunodeficiencies, and cutaneous manifestations, including photosensitivity, poikiloderma, and telangiectatic erythema. (medscape.com)
  • Barabasi A, Oltvai ZN: Network biology: understanding the cell’s functional organization. (crossref.org)
  • Kamburov A, Pentchev K, Galicka H, Wierling C, Lehrach H, Herwig R: ConsensusPathDB: toward a more complete picture of cell biology. (crossref.org)
  • Along with technological developments, single-cell biology brings new conceptual challenges. (nature.com)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • Thus, alterations inside the ICAT ratios for these proteins are most likely on account of indirect effects, for instance MMPI modulation from the protease internet (91, 92), or maybe these proteins are present inside the conditioned medium secretome only when bound to proteins which are themselves reduced in amount following decreased shedding upon MMPI treatment (Fig. 1B and D). Accumulation of substrates in cell membranes upon MMPI remedy. (translateinthetownships.com)
  • Through integrative comparative analysis, we define a consensus vocabulary and a consistent set of gene signatures discriminating against the transcriptomic cell types and subtypes of the human prefrontal cortex. (nature.com)
  • In yeast, de novo peroxisome formation was generally studied in peroxisome-deficient pex3 cells following reintroduction of the PEX3 gene. (rug.nl)
  • We have been fascinated by the process of yeast mating-type gene switching, in which cells replace about 700 bp of Ya or Y-specific DNA sequences at the MAT locus by recombining with one of two donor loci, called HMLDescription: image3 and HMRa. (brandeis.edu)
  • Accordingly, the epistatic relationship between cell death and SA accumulation, analyzed in crosses between lesion-mimic mutants (spontaneous lesion formation) and the transgenic nahG line (depleted in SA) places the SA activity in a feedback loop downstream and upstream of cell death. (springer.com)
  • Exciting advances have been made in the identification of cellular protective functions and cell death suppressors that might operate in HR. Moreover, the spatio-temporal patterns of the SA accumulation (non-homogeneous distribution, biphasic kinetics) described in some HR lesions, may also reveal important clues for unraveling the complex cellular network that tightly balances pro- and anti-death functions in the hypersensitive cell death. (springer.com)
  • SDS-PAGE was performed on cell lysate samples on a 4C12% gradient gel (Nu-PAGE, Invitrogen), followed by transfer to nitrocellulose membranes and immunoblotting as previously explained (2, 3) for expression of active phosphorylated AURKA using a rabbit anti-pThr288-AURKA antibody and then for -actin. (researchreportone.com)
  • ACTIONet provides a robust, reproducible, and highly interpretable single-cell analysis platform that couples dominant pattern discovery with a corresponding structural representation of the cell state landscape. (nature.com)
  • Dissecting the cellular heterogeneity embedded in single-cell transcriptomic data is challenging. (nature.com)
  • Mitochondria are multifaceted organelles with key roles in anabolic and catabolic metabolism, bioenergetics, cellular signalling and nutrient sensing, and programmed cell death processes. (nature.com)
  • The microtubule and microfilament cytoskeleton is essential for many dynamic cellular process including: cell division, cytoplasmic streaming, cellular architecture and morphogenesis. (purdue.edu)
  • Single-cell sequencing of primate preimplantation embryos reveals chromosome elimination via cellular fragmentation and blastomere exclusion. (ohsu.edu)
  • HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
  • ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
  • This mutation is acquired during a person's lifetime and is present only in tumor cells. (medlineplus.gov)
  • Costanzo, M., Baryshnikova, A., Bellay, J., Kim, Y., Spear, E., Sevier, C., Ding, H., Koh, J., Toufighi, K., Mostafavi, S., Prinz, J., Onge, R., VanderSluis, B., Alizadeh, S., Bahr, S., Brost, R., Chen, Y., Cokol, M., Deshpande, R., Li, Z., Li, Z.-Y., Lian, W., Marback, M., Paw, J., San Luis, B.-J., Shuteriqi, E., Tong, A., van Dyk, N., Wallace, I., Whitney, J., Weirauch, M., Zhong, G., Zhu, H., Houry, W., Brudno, M., Ragibizadeh, S., Papp, B., Roth, F., Giaever, G., Nislow, C., Troyanskaya, O., Bussey, H., Bader, G., Gingras, A., Morris, Q., Kim, P., Kaiser, C., Myers, C., Andrews, B., Boone, C.: The Genetic Landscape of a Cell. (crossref.org)
  • The genetic landscape of a cell. (crossref.org)
  • They observed high glucose consumption and large amounts of lactate excretion from cancer cells compared with normal cells, which oxidised glucose using mitochondria. (researchgate.net)
  • It was therefore assumed that cancer cells were generating energy using glycolysis rather than mitochondrial oxidative phosphorylation, and that the mitochondria were dysfunctional. (researchgate.net)
  • Advances in research techniques since then have shown the mitochondria in cancer cells to be functional across a range of tumour types. (researchgate.net)
  • cells, which oxidised glucose using mitochondria. (researchgate.net)
  • Thus, citric acid cycle intermedi- ates are not used for adenosine triphosphate (ATP) production and are shuttled out of the mitochondria, providing precursors for nucleotide, amino acid, and lipid synthesis path- ways for the dividing cell [13]. (who.int)
  • Controls for the immunoprecipitation reaction were performed in the absence of the immunoprecipitating antibody and in the absence of lysate for both cell types. (researchreportone.com)
  • This dysregulation of transcription leads to uncontrolled growth and division (proliferation) and abnormal maturation and survival of cells, causing tumor development. (medlineplus.gov)
  • We discovered that myocilin increased cell proliferation and survival. (nih.gov)
  • Gavin A, Aloy P, Grandi P, Krause R, Boesche M, Marzioch M, Rau C, Jensen LJ, Bastuck S, Dümpelfeld B, Edelmann A, Heurtier M, Hoffman V, Hoefert C, Klein K, Hudak M, Michon A, Schelder M, Schirle M, Remor M, Rudi T, Hooper S, Bauer A, Bouwmeester T, Casari G, Drewes G, Neubauer G, Rick JM, Kuster B, Bork P, Russell RB, Superti-Furga G: Proteome survey reveals modularity of the yeast cell machinery. (crossref.org)
  • We implement this concept in ACTIONet, a comprehensive framework that combines archetypal analysis and manifold learning to provide a ready-to-use analytical approach for multiresolution single-cell state characterization. (nature.com)
  • Single-cell genomic technologies are revolutionizing the way tissues and cell populations are experimentally interrogated. (nature.com)
  • We discuss how emerging technologies can assist the detection of new mitochondrial proteins, reveal their patterns of expression across diverse tissues and cell types, and provide key information on proteoforms. (nature.com)
  • Osei P, Northcote-Smith J, Fang J, Singh K, Ortu F, Suntharalingam K . The Bulk Breast Cancer Cell and Breast Cancer Stem Cell Activity of Bi-nuclear Copper(II)-Phenanthroline Complexes. (le.ac.uk)
  • We are interested in investigating the potential neuroprotective benefits of stem cell transplantation, which has produced encouraging results in different models of CNS degeneration. (nih.gov)
  • Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
  • Stem cell niche. (bvsalud.org)
  • The major breakthrough is the identification of a biomarker of HIV persistent cells in vivo. (europa.eu)
  • We describe using this system to quantitatively measure adhesion of the inflammatory cells - monocytic THP-1 cell line to the HUVEC monolayer. (bvsalud.org)
  • Cells were seeded onto coverslips at 2.5 105 cells/cm2 and managed in culture for 4C5 days to form a confluent monolayer. (researchreportone.com)
  • This regulation contributes to kidney carcinoma V-ATPase-mediated extracellular acidification and cell migration. (researchreportone.com)
  • Tunneling nanotube (TNT)-like structures facilitate a constitutive, actomyosin-dependent exchange of endocytic organelles between normal rat kidney cells. (uib.no)
  • Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. (nih.gov)
  • Recombination between homologous sequences is a fundamentally important process both in meiosis and in mitotic cells. (brandeis.edu)
  • To this end we have expressed the site-specific HO endonuclease in meiotic cells so that we can compare recombination events at the same loci where we have used HO to stimulate recombination in mitotic cells. (brandeis.edu)
  • This ulcerated nodule of localized primary cutaneous CD30+ anaplastic large cell lymphoma is relatively nonspecific in appearance. (medscape.com)
  • PC-ALCL is one of the primary cutaneous CD30 + T-cell lymphoproliferative disorders, a wide spectrum of disease, with lymphomatoid papulosis (LyP) at the benign end of the spectrum and PC-ALCL at the malignant end. (medscape.com)
  • The term 'necrotic' alludes to either apoptotic or necrotic cell death. (springer.com)
  • We present a simple and quantitative assay system that accurately models human endothelium by use of primary human umbilical vein endothelial cells (HUVECs) in cell culture plates coated with gelatin , a matrix that mimics basal lamina , the matrix that is tightly associated with the vascular endothelium and is critical for its proper function. (bvsalud.org)
  • Finally, S175A-expressing cells experienced decreased migration in a wound-healing assay compared with cells expressing wild-type or a phospho-mimetic Ser-175-to-Asp (S175D) mutant A subunit. (researchreportone.com)
  • In particular, we focus on the hypothesis that the macrophage is an important cell type in the propagation of inflammation and induction of insulin resistance in obesity. (jci.org)
  • In the USA, obesity has recent- obesity is associated with progres- aberrations of cancer cells, summa- ly surpassed tobacco use as the sion but not incidence [9]. (who.int)
  • In both genetic and diet-induced models of insulin resistance, CHOP deficiency improved β cell ultrastructure and promoted cell survival. (jci.org)