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  • proteolytic
  • The present study demonstrates that IGF-1 regulates the proteolytic activity of MMP-2 produced by MIO-M1 cells increasing their migratory capacity in a way that seems to be associated with IGF-1R expressed in these cells. (arvojournals.org)
  • The ECM is affected by the slower synthesis and turnover of new components, as well as greater enzyme proteolytic degradation of collagen and elastin fibers that occur from the 40s onwards. (happi.com)
  • We have identified that laminin-5 undergoes proteolytic processing of two of its three chains, via mammalian Tolloid, a metalloprotease of the astacin family. (stanford.edu)
  • basement membrane
  • the matrix metalloproteinases degrade components of the basement membrane, collagen and laminin. (cardiff.ac.uk)
  • To test whether the effect of these basement membrane components on cell migration is protease-dependent, we studied their interaction with MMP2 (gelatinase A, 72-kD type IV collagenase), a protease that is concentrated along basement membranes at sites of tissue remodeling ( 1 ) and at the leading edge of invading tumors ( 1 , 2 , 6 ). (sciencemag.org)
  • attachment
  • To overcome ECM barriers, advancing cells may focus proteases such as metalloproteases ( 2 ) or protease activators such as urokinase ( 1 , 2 ) at their leading edge, where complex proteolysis ( 1 ) can direct migration, preserve ECM attachment, or avoid unwanted tissue damage. (sciencemag.org)
  • Cell attachment to, spreading on, and movement along the ECM are integrin dependent ( 1 , 2 ). (aacrjournals.org)
  • Once firm cell attachment is achieved, the hydrogel is quickly removed to expose a cell-free region to study cell migration/closure. (docplayer.net)
  • Overnight cell attachment is not recommended in that it may lead to over confluent wells and compromise the Radius Gel Spot. (docplayer.net)
  • embryonic
  • During early embryonic development, cranial neural crest cells emerge from the developing mid- and hindbrain. (nih.gov)
  • 2 Introduction Cell migration is a highly integrated, multistep process that orchestrates embryonic morphogenesis, tissue repair and regeneration. (docplayer.net)
  • chemokine
  • The T-cell lines showed a peak response at a chemokine concentration of 10-50 ng/ml, whereas the T-cell clone responded optimally at 100 ng/ml. (diva-portal.org)
  • Stimulation of infiltration of T-cell lines into collagen by the chemokine SDF-1α was inhibited by somatostatin, a neuropeptide with immunosuppressive properties. (diva-portal.org)
  • Finally, a possible physiological mechanism for modulation of the chemokine-stimulated 3-D migration was demonstrated. (diva-portal.org)
  • neuronal
  • These cells self-renew and are multipotent as they give rise to neuronal, astrocytic, and oligondendrocytic cells ( Reynolds and Weiss, 1992 ). (jneurosci.org)
  • migratory
  • In this work we evaluate the effect of IGF-1 on the regulation of MMPs and on the migratory capacity of an immortalized human Müller cells line, MIO-M1. (arvojournals.org)
  • Our findings suggest an involvement of SRF in cell migratory processes in multicellular organisms. (rupress.org)
  • The Radius Cell Migration Assay Kit utilizes a proprietary 24-well plate, which has been pre-coated with Laminin, to monitor the migratory properties of cells. (docplayer.net)
  • Adherent migratory cell lines and culture medium 2. (docplayer.net)
  • organization
  • In this Review , Rodrigo G. Arzate-Mejía, Félix Recillas-Targa and Victor G. Corces discuss evidence linking CTCF to the control of developmental processes in various cell and tissue types through 3D organization of the genome. (biologists.org)
  • tissues
  • If these sorts of enzymes are being activated during transendothelial migration, perhaps they're activated for penetration of tissues. (cardiff.ac.uk)
  • Laminin-10 is a widely expressed molecule found in a number of epithelial tissues. (stanford.edu)
  • Chimera galectins, in turn, consist of one CRD connected to a collagen-like sequence formed by Pro-Gly-Tyr tandem repeats and an N-terminal domain (Figure 1 A). Some galectins are present in a variety of tissues, while others have a more specific localization. (frontiersin.org)
  • Versican is completely absent from tissues invaded by neural crest cells and extending axons. (biologists.org)
  • antigen
  • T cells traffic around the body in and out of secondary lymphoid organs, searching for antigen which becomes sequestered there. (cardiff.ac.uk)
  • indicate
  • Moreover, cell-based enzymatic activity assays for PLA 2 indicate that peritoneal mesothelial cells have strong constitutive PLA 2 activity. (aacrjournals.org)
  • progression
  • These findings suggest that therapeutics targeted toward G i -couplers that are selectively expressed in breast cancer cells could prove beneficial in halting the progression of invasive breast cancer. (aacrjournals.org)
  • peptide
  • We look at the mouse model of infection which is as close to a natural infection as possible, as opposed to using peptide-primed dendritic cells to activate T cells. (cardiff.ac.uk)
  • The crystal structure of a complex between the I domain of integrin alpha2beta1 and a triple helical collagen peptide containing a critical GFOGER motif has been determined. (sdbonline.org)
  • surfaces
  • Rabbit lens epithelial cells did not adhere to ovalbumin-coated surfaces at any concentration tested. (arvojournals.org)
  • In the frog embryo, this movement is coordinated into an organised pattern where cells use the surfaces of their slower or stationary neighbours to propel past each other, and then slow down to return the favour. (elifesciences.org)
  • stimulates
  • Application of RANTES, MIP-1α, MIP-1β, or MCP-1 chemokines that are secreted by leukocytes (16) stimulates chemotaxis of MCF-7 cells. (aacrjournals.org)