• Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). (novusbio.com)
  • Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. (novusbio.com)
  • FKRP Protein LS-G110010 is an Over-Expression Lysate FKRP with Myc-DDK (Flag) tag(s). (lsbio.com)
  • We also observed that expression of Cp protein in mouse muscle resulted in apoptosis and inflammation of muscle cells. (cdc.gov)
  • Interestingly, real-time PCR and translation inhibition studies revealed that survivin was over-expressed partially through the up-regulation of protein translation instead of gene transcription in A549 cancer cells. (biomedcentral.com)
  • In addition, 17-AAG-treated A549, HONE-1 and HT-29 cells showed reduced proteasomal activity while inhibition of 26S proteasome activity further increased the amount of survivin protein in cells. (biomedcentral.com)
  • Heat shock protein 90 (Hsp90) is a molecular chaperone that assists the correct folding and stabilization of various proteins in cells. (biomedcentral.com)
  • Unlike other IAPs, survivin is a bifunctional protein that functions as a key regulator of mitosis and inhibitor of programmed cell death. (biomedcentral.com)
  • Real-time PCR and western blot were used to detect ENDOD1 mRNA and protein expression in normal prostatic epithelial and PCa cell lines. (biomedcentral.com)
  • Western blot showed that androgen-sensitive LNCaP cells had the highest protein expression level of ENDOD1, whereas castration-resistant PCa cell lines PC3 and DU145 had significantly lower protein levels. (biomedcentral.com)
  • The aim of this study was to assess the role that neuronal injury or, vice versa , cell integrity could have in facilitating interneuronal α-synuclein transfer and consequent protein spreading in an in vivo animal model. (biomedcentral.com)
  • Viral vectors carrying the DNA for human α-synuclein were injected into the rat vagus nerve to trigger protein overexpression in the medulla oblongata and consequent spreading of human α-synuclein toward pons, midbrain and forebrain. (biomedcentral.com)
  • Elucidation of the mechanisms involved in the transfer of α-syn (and/or pathological forms of it) from donor to recipient cells bears significant pathogenetic implications and could provide clues for therapeutic intervention targeting protein spreading. (biomedcentral.com)
  • Suggested positive control: human heart protein, antigen standard for PDK1 (transient overexpression lysate). (thermofisher.com)
  • The BRCA2 R3052W mutated protein exacerbates genome instability, is unable to rescue homology-directed repair, and fails to complement cell survival following exposure to PARP inhibitors and crosslinking drugs. (frontiersin.org)
  • p62 is a multifunctional protein that mediates cell signaling pathways, autophagy and tumorigenesis, and participates in important regulation processes at the intersection between autophagy and cancer. (spandidos-publications.com)
  • Cells overexpressing p62 showed a higher cell death rate than cells not expressing this protein. (spandidos-publications.com)
  • Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
  • TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, L-selectin, and alpha-cleavage of amyloid protein precursor (APP). (qedbio.com)
  • The relationship between lysate protein concentration from paclitaxel-treated and hydroxyurea-treated HeLa cells and the absorbance at 450 nm using the PathScan ® RP Phospho-Aurora A (Thr288) Sandwich ELISA Kit #41333 is shown in the upper figure. (cellsignal.com)
  • Western Blot: WW domain binding protein 5 Antibody [NBP1-84208] - Analysis in control (vector only transfected HEK293T lysate) and WBP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells). (antibodypedia.com)
  • Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody [NBP1-84208] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells. (antibodypedia.com)
  • The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. (prosci-inc.com)
  • SAM domain and HD domain containing protein 1 (SAMHD1) is a deoxynucleotide triphosphohydrolase (dNTPase) that inhibits retroviruses by depleting intracellular deoxynucleotide triphosphates (dNTPs) in non-cycling myeloid cells. (oncotarget.com)
  • Cell-fate-determinant molecule NUMB-interacting protein (TBC1D15) is overexpressed and contributes to p53 degradation in TICs. (nature.com)
  • Here, we demonstrate that the S phase checkpoint protein CHK1 is maintained in a low phosphorylation state in Spi1/PU.1-overexpressing cells and provide evidence that this is not due to negative control of its primary kinase ATR. (oncotarget.com)
  • The authors have previously reported that the Salmonella typhimurium SigD protein, a phosphatidylinositol phosphatase involved in invasion of the host cell, inhibits yeast growth, presumably by depleting an essential pool of phosphatidylinositol 4,5- bis phosphate, and also that a catalytically inactive version, SigD R468A , was able to arrest growth by a different mechanism that involved disruption of the actin cytoskeleton. (microbiologyresearch.org)
  • Modulation of chloride secretory responses and barrier function of intestinal epithelial cells by the Salmonella effector protein SigD. (microbiologyresearch.org)
  • PM 2.5 increased the mRNA and protein expression of M1 markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. (medsci.org)
  • Overexpression of miR-146a-3p downregulated the expression of SIRT1 protein in untreated RAW264.7 cells. (medsci.org)
  • Importantly, inhibition of miR-146a-3p upregulated SIRT1 protein and suppressed M1 polarization in PM 2.5 treated RAW264.7 cells. (medsci.org)
  • Full length human recombinant protein of human MAPK1 produced in HEK293T cell. (thermofisher.com)
  • GCM1 is a DNA-binding protein with a gcm-motif (glial cell missing motif). (avivasysbio.com)
  • Quality control test: Transient overexpression cell lysate was tested with Anti-STX2 antibody by Western Blots. (novusbio.com)
  • Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. (novusbio.com)
  • The localization pattern of capsid expression was analyzed by immunofluorescent assay in HeLa, 293-T, RD, or SH-SY5Y cells by using anti-His tag antibody as described ( 6 ). (cdc.gov)
  • Treatment of LMP1/CD40-expressing lymphomatous mice with an anti-PD-L1 monoclonal antibody induced tumor regression with decreased spleen content, activation and proliferation rate of B-cells as well as a marked increase in T-cell activation, as assessed by CD62L and CD44 expression. (springer.com)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with 1131 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). (qedbio.com)
  • Incubation of cell lysates and detection antibody on the coated microwell plate forms a sandwich with phospho-Aurora A (Thr288) in a single step. (cellsignal.com)
  • For short periods of storage (days) store at 4°C. For longer periods of storage, store STC1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles. (prosci-inc.com)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • Capsid gene delivery into the striatum of mouse brain or interskeletal muscle resulted in cell death and inflammation, likely through capsid-induced apoptosis in vivo. (cdc.gov)
  • In vitro apoptosis in individual cells was determined by terminal desoxyriboxyl-desoxyriboxyl transferase-mediated DVTP nick-end labeling (TUNEL) assay with the In Situ Cell Death Assay Kit (Roche Diagnostic Corp., Indianapolis, IN) and visualized by fluorescent microscopy. (cdc.gov)
  • It inhibits the apoptosis of cancer cells by inhibiting caspases, and also promotes cancer cell growth by stabilizing microtubules during mitosis. (biomedcentral.com)
  • Protects cells against apoptosis in response to hypoxia and oxidative stress. (thermofisher.com)
  • Treatment of carfilzomib followed by selinexor caused selinexor-sensitive and selinexor-resistant cell lines to be more sensitive to selinexor as determined by an increase in apoptosis. (aacrjournals.org)
  • The resulting damage cannot be overcome by the antioxidant system to protect the cell from oxidative damage, leading to necrosis, apoptosis, or autophagy of the target cell and tissue ( 10 , 11 ). (spandidos-publications.com)
  • These results suggest that TIA1 silencing in ESCC cells contributes to cell cycle arrest in the G1-S checkpoint and the induction of apoptosis. (mdm-inhibitors.com)
  • Ibudilast showed modest anti-proliferative activity however, when combined with TMZ, significant synergism was observed, resulting in cell cycle arrest and apoptosis. (nature.com)
  • Expression system for standard: Survivin(also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. (innov-research.com)
  • In vivo studies further confirmed the inhibitory effect of celastrol on the expression of HIF-1α proteins, leading to a decreased growth of Hep3B cells in a xenograft tumor model. (spandidos-publications.com)
  • Likewise TIA1 proteins overexpression was seen in most of cancers cells weighed against regular mucosa (Supplementary Amount S2B). (mdm-inhibitors.com)
  • Using this tag, we prepared mild detergent lysates from transgenic mouse brain cortical membrane preparations and isolated a number of previously identified APP-interacting proteins. (jneurosci.org)
  • Here, we show that APP can coimmunoprecipitate NEEP21 from nontransgenic mouse brain and from mammalian cells stably coexpressing both proteins. (jneurosci.org)
  • In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
  • The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
  • Here, we demonstrate that the dNTPase activity of SAMHD1 is regulated by acetylation, which promotes cell cycle progression in cancer cells. (oncotarget.com)
  • This family of RTKs has been highly implicated in the formation and progression of various cancers via aberrant overexpression, kinase activation, and mutation [1] , [2] . (plos.org)
  • By exogenously modulating its activity, we demonstrate that PP1α is required to maintain CHK1 in a dephosphorylated state and, more importantly, that it is responsible for the accelerated replication fork progression in Spi1/PU.1-overexpressing cells. (oncotarget.com)
  • EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
  • These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
  • Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. (microbiologyresearch.org)
  • The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. (innov-research.com)
  • This cell series responds inside a physiologic manner to growth factor-induced cell cycle progression (15). (cell-signaling-pathways.com)
  • CONCLUSION: Collectively, these results indicate that PFKP expression in melanoma cells increases proliferation and glycolytic activity in vitro and promotes tumorigenesis in vivo, suggesting that suppression of PKFP and inhibition of glycolysis may potently suppress melanoma progression. (bvsalud.org)
  • HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. (origene.com)
  • HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. (novusbio.com)
  • (B) Western blot of total cellular lysates from DLD-1 BRCA2 −/− cells stably transfected with full-length BRCA2 cDNA constructs: BRCA2 Wild Type (WT) and BRCA2 R3052W (1 and 2 correspond to two independent clones). (frontiersin.org)
  • (F) Western blot of total cellular lysates from DLD-1 parental cells (these cells express a wild-type allele of BRCA2) stably transfected with R3052W (3 and 5 correspond to two independent clones) full-length 2XMBP-BRCA2 cDNA constructs. (frontiersin.org)
  • In a screen for suppressors of SigD R468A -induced growth arrest by overexpression of a yeast cDNA library, the Cdc42 GTPase was isolated. (microbiologyresearch.org)
  • Expected and potentially novel resistance genes are enriched in the top hits and are validated using individual sgRNA as well as cDNA overexpression. (cdc.gov)
  • Genome-scale GOF screening approaches have largely remained limited to the use of cDNA library overexpression systems. (cdc.gov)
  • In HepG2 cells, exendin-4-reversed lipid deposition induced by palmitate was hampered when SIRT1 was silenced by SIRT1 RNA interference. (diabetesjournals.org)
  • B. Relative p21 mRNA levels measured by qPCR in HepG2 cells transduced with rAd.A20, rAd.7Zn and rAd.Nter for 48h. (cell-signaling-pathways.com)
  • C. Representative IB Western blot of cell lysates from rAd.A20, rAd.7Zn and rAd.Nter HepG2 cells treated with TNF (200 U/mL) for 15 min. (cell-signaling-pathways.com)
  • MTT assays were employed to determine the effect of cell proliferation. (biomedcentral.com)
  • Meanwhile, overexpression of ENDOD1 suppressed cell proliferation, induced G0/G1 cell cycle arrest and inhibited cell migration and invasion. (biomedcentral.com)
  • Conversely, siRNA-mediated silencing of ENDOD1 promoted cell proliferation, migration and invasion. (biomedcentral.com)
  • Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. (thermofisher.com)
  • K405R mutant expressing cancer cells have reduced G1/S transition and slower proliferation compared to wildtype. (oncotarget.com)
  • Collectively, these findings suggest that SAMHD1 acetylation enhances its dNTPase activity and promotes cancer cell proliferation. (oncotarget.com)
  • Number 2 Subcellular distribution of the TIA1 isoforms Involvement of TIA1 in ESCC cell proliferation To gain insight into the potential function Lidocaine (Alphacaine) of TIA1 whose overexpression could be associated with esophageal carcinogenesis we 1st tested the effects of small interfering RNA (siRNA) focusing on TIA1 on cell proliferation. (mdm-inhibitors.com)
  • By silencing endogenous TIA1 using three different siRNAs cell proliferation was significantly suppressed in KYSE140 KYSE180 and TE4 cells. (mdm-inhibitors.com)
  • Number 3 Effects of TIA1 knockdown on cell proliferation We next launched a TIA1[v1]- or TIA1[v2]-expressing retrovirus into KYSE190 and KYSE2270 cells expressing relatively lower levels of endogenous TIA1 to determine the effects of exogenously indicated TIA1 isoforms. (mdm-inhibitors.com)
  • Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
  • Outcomes N-terminal and 7Zn C-terminal Domains of A20 Separately Promote Hepatocyte Proliferation within a p21-unbiased Fashion We examined cell proliferation in non-transduced (C), rAd.A20, rAd.Nter, rAd.7n, and rAd.gal-transduced NMuLi cells. (cell-signaling-pathways.com)
  • This indicated that self-employed overexpression of Nter or 7Zn raises proliferation in NMuLi cells. (cell-signaling-pathways.com)
  • NMuLi cells were transduced with rAd.A20, rAd.7Zn and rAd.Nter, serum starved for 24h to synchronize their cell cycle, then supplemented with 10% FBS enriched medium to drive cell proliferation. (cell-signaling-pathways.com)
  • CCK-8 assay was used to assess cell proliferation. (bvsalud.org)
  • Knockdown of PFKP in human melanoma cells suppressed cell proliferation and reduced ECAR, ATP levels, and lactic acid levels, while overexpression of PFKP displayed the opposite effects. (bvsalud.org)
  • Inhibitory effects on cell proliferation, glycolysis, and tumorigenesis due to PFKP knockdown were further augmented upon treatment with the glycolysis inhibitor 2-deoxy-D-glucose (2-DG). (bvsalud.org)
  • Right -Over-expression Lysate for Proteasome 19S 10B. (novusbio.com)
  • The mechanistic effects of selinexor in sarcomas as a monotherapy and in combination with proteasome inhibitor, carfilzomib, across a panel of cell lines in vitro and few in xenograft mouse models were investigated. (aacrjournals.org)
  • This effect can be further enhanced in relatively selinexor-resistant sarcoma cell lines by pretreatment with the proteasome inhibitor carfilzomib. (aacrjournals.org)
  • RD cells transfected with pcWNV-Cp-DJY or pcDNA3.1 plasmid DNA were processed for transmission electron microscope analysis as described ( 7 , 8 ). (cdc.gov)
  • The pre-clinical LMP1/CD40-expressing transgenic mouse model is characterized by B-cell specific CD40 signaling responsible for NF-κB continuous activation with a spleen monoclonal B-cell tumor after 1 year in 60% of cases. (springer.com)
  • Syntaxin 2 was first identified as a factor that promotes branching morphogenesis in mammary epithelial cells. (novusbio.com)
  • Real-time PCR data indicated that ENDOD1 mRNA expression was markedly increased in LNCaP and 22Rv1 cells and decreased in PC3 and DU145 cells compared to the normal epithelial cells RWPE1. (biomedcentral.com)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • We report that the WNV-capsid (Cp) by itself induces rapid nuclear condensation and cell death in tissue culture. (cdc.gov)
  • Nuclear localized HER family receptor tyrosine kinases (RTKs) have been observed in primary tumor specimens and cancer cell lines for nearly two decades. (plos.org)
  • Requirement of Cdc42 for Salmonella typhimurium -induced cytoskeletal reorganization and nuclear responses in cultured cells. (microbiologyresearch.org)
  • Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. (thermofisher.com)
  • Lysates are guaranteed for 6 months from date of receipt. (novusbio.com)
  • Lysate samples are stable for 12 months from date of receipt when stored at -20°C. (origene.com)
  • Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. (innov-research.com)
  • This expression was dependent on activation of either NF-κB, JAK1/JAK2 or BTK pathways since these pathways were activated in tumor B-cells and ex vivo treatment with the inhibitory molecules PHA-408, ruxolitinib and ibrutinib led to decrease of its expression. (springer.com)
  • However, overexpression of Gyp1p was inhibitory in combination with a subset of secretory mutants including sec4-8 and several ypt1 mutants. (embl.de)
  • Celastrol showed a potent inhibitory activity against HIF-1 activation induced by hypoxia in various human cancer cell lines. (spandidos-publications.com)
  • Overexpression of macrophage migration inhibitory factor (MIF) was identified in human GBM specimens that were MGMT methylated but showed poor survival. (nature.com)
  • First, γδ T cells exposed to complicated cytokine networks are potentially affected by multiple inhibitory mechanisms. (explorationpub.com)
  • Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate.This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. (prosci-inc.com)
  • Overexpression of human stanniocalcin 1 in mice produces high serum phosphate levels, dwarfism, and increased metabolic rate. (prosci-inc.com)
  • This Human Survivin (BIRC5) ELISA Kit from Innovative Research is intended for quantitative detection of human Survivin in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). (innov-research.com)
  • Avoid freeze-thaw cycles. (novusbio.com)
  • Avoid repeated freeze-thaw cycles after reconstitution. (origene.com)
  • Store at -80°C. Avoid freeze-thaw cycles. (lsbio.com)
  • Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. (origene.com)
  • You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). (novusbio.com)
  • Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. (novusbio.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. (novusbio.com)
  • PDT uses a modality-based photosensitizer, which selectively affects cancer cells, using excitation and light-absorption in the presence of oxygen to produce a high concentration of reactive oxygen species (ROS), such as singlet oxygen and other free radicals ( 7 - 9 ). (spandidos-publications.com)
  • The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. (origene.com)
  • We have combined the proteomic analysis of Xenopus laevis in vitro-assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. (rupress.org)
  • Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. (rupress.org)
  • Loading: 10 ug of HeLa WT cell lysates or TACE KO cell lysates. (qedbio.com)
  • HeLa cells were treated with paclitaxel (100 nM) or hydroxyurea (4 mM) for 18 hr at 37°C and then lysed. (cellsignal.com)
  • In vitro GAP activity towards RhoA, Rac1 and Cdc42 is not a prerequisite for YopE induced HeLa cell cytotoxicity. (microbiologyresearch.org)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • Second, growth inhibition by SigD R468A was mediated by a cell cycle arrest in G2 dependent on the Swe1 morphogenetic checkpoint, but SigD-induced growth inhibition was cell cycle independent. (microbiologyresearch.org)
  • Studies using pancreatic exocrine acinar cells, in which the exocytic pathways are well defined, show primary localization of syntaxin 2 to the apical plasma membrane. (novusbio.com)
  • From studies of APP trafficking and metabolism, the following pathways have emerged: in the first, a fraction of APP molecules residing on the cell surface are processed by ADAM/TACE "sheddases" N-terminal to the ectodomain-transmembrane domain to generate an 83 aa membrane-tethered stub, termed α-CTF. (jneurosci.org)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • Deletion of the GYP1 gene or overexpression of Gyp1p did not alter the growth rate of yeast. (embl.de)
  • GCM1 is a homolog of the Drosophila glial cells missing gene (gcm). (avivasysbio.com)
  • Prior to SDS-PAGE fractionation, boil the lysate for 5-10 minutes. (lsbio.com)
  • Instead, targeting Hsp90 with small molecule inhibitors will induce the over-expression of survivin in certain cancer cell lines and subsequently enhances the ability of cell survival in drug-treated situations. (biomedcentral.com)
  • Autophagy contributes to the prolonged survival of tumor cells, whereas defects in autophagy play a critical role in tumorigenesis ( 15 , 16 ). (spandidos-publications.com)
  • Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)
  • At the functional level, down-regulation of survivin by siRNA further increased the drug sensitivity to 17-AAG in the tested cancer cell lines. (biomedcentral.com)
  • Knockdown of PFKP by siRNA and overexpression of PFKP were performed to evaluate its functions in vitro. (bvsalud.org)
  • Irradiated Ce6 produced singlet oxygen which rapidly induces tumor cell death. (spandidos-publications.com)
  • Zhong Y, Liao J, Hu Y, Wang Y, Sun C, Zhang C, Wang G. PM 2.5 Upregulates MicroRNA-146a-3p and Induces M1 Polarization in RAW264.7 Cells by Targeting Sirtuin1. (medsci.org)
  • Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
  • In vivo, knockdown of PFKP in melanoma cells markedly reduced tumorigenesis. (bvsalud.org)
  • IL-6 levels significantly increased in all groups following TNF/LPS, as compared to corresponding non-stimulated cells (6.5 to 9.9 fold, Fig. 2A). (cell-signaling-pathways.com)
  • Mitotic chromosome segregation requires the coordination of both regulatory and mechanical molecular machines and culminates in the delivery of two complete sets of chromosomes to two daughter cells. (rupress.org)
  • Unfortunately, scientists have yet to be able to locate specific features of malignant and immune cells that would make them uniquely targetable (barring some recent examples, such as the Philadelphia chromosome as targeted by imatinib). (justia.com)
  • The purpose of this study is to determine whether targeting Hsp90 can alter survivin expression differently in different cancer cell lines and to explore possible mechanisms that cause the alteration in survivin expression. (biomedcentral.com)
  • Here, we demonstrated that Hsp90 inhibitors, geldanamycin and 17-AAG, induced the over-expression of survivin in three different human cancer cell lines as shown by Western blotting. (biomedcentral.com)
  • For example, over-expression of survivin has been shown to induce drug resistance against anti-mitotic compounds by stabilizing microtubule network in vincristine/colchicine-resistant oral cancer cells and down-regulation of it restores drug sensitivity to those compounds in the same cell line [ 9 ]. (biomedcentral.com)
  • Interestingly, a recent report suggests that over-expression of survivin may also enhance DNA double-strand breaks (DBD) repair capability in radiation-treated oral cancer cells by up-regulating the molecular sensor of DNA damage, Ku70 [ 11 ]. (biomedcentral.com)
  • NF-κB, one of the most cited transcription factor in B-cell lymphomas, is able to increase tumor cell expression of PD-L1 either directly or indirectly [ 3 ]. (springer.com)
  • However, whether the efficacy of PDT depends on the expression level of p62 in colorectal cancer cell lines is not known. (spandidos-publications.com)
  • The present study aimed to examine the role of p62 expression levels in chlorin e6‑based PDT in colorectal cancer cells. (spandidos-publications.com)
  • Further, the overexpression of HER3 enhanced cyclin D1 mRNA expression, while HER3 deleted of its identified TADs was hindered at doing so. (plos.org)
  • Notably, we found that the expression of the CHK1 phosphatase PP1α is increased in Spi1/PU.1-overexpressing cells. (oncotarget.com)
  • In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
  • First, expression of SigD R468A caused accumulation of large unbudded cells and loss of septin organization, while SigD expression caused none of these effects. (microbiologyresearch.org)
  • at a multiplicity of contamination (MOI) of 50C200 Troxerutin distributor plaque-forming models per cell (pfu/cell), leading to transgene expression in 95% of cells without toxicity (14, 15) (Fig. S1). (cell-signaling-pathways.com)
  • RESULTS: We showed that PFKP promotes EGFR activation-induced PD-L1 expression in human GBM cells. (bvsalud.org)
  • CONCLUSION: These findings provide new mechanistic insight into the regulation of PD-L1 expression by a non-metabolic function of PFKP on tumor cells. (bvsalud.org)
  • Importantly, Fbxo7-deficient cells have reduced Cdk6 activity, and hematopoietic and lymphocytic cells show high expression and significant dependency on Fbxo7. (bvsalud.org)
  • Overexpression of DEFB1 in IAV-infected HBEpCs led to increased NF-B expression. (cdc.gov)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
  • In particular, the present invention provides methods for enhancing the effectiveness of chemotherapy by inducing differential stress resistance in normal cells and cancer cells via short-term starvation, cell growth inhibitors, or reduced caloric or glucose intake. (justia.com)
  • The amino-terminal non-catalytic region of Salmonella typhimurium SigD affects actin organization in yeast and mammalian cells. (microbiologyresearch.org)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • Desk 1 Association between clinicopathological features and TIA1 appearance Desk 2 Cox proportional threat regression evaluation for overall success Appearance of TIA1 in ESCC cell lines mRNA overexpression weighed against the esophagus was also discovered in 30 of 45 ESCC cell lines by quantitative real-time PCR (qPCR Supplementary Amount S2A). (mdm-inhibitors.com)
  • We repurposed ibudilast, a specific MIF inhibitor, and treated patient derived cell lines. (nature.com)
  • However, the clinical application of tumor-infiltrating γδ T cells has certain limitations. (explorationpub.com)
  • Finally, it retrospectively analyze the activation strategies and clinical relevance of existing γδ T cell adoptive immunotherapies. (explorationpub.com)
  • Although SAMHD1 is expressed ubiquitously throughout the human body, the molecular mechanisms regulating its enzymatic activity and function in non-immune cells are relatively unexplored. (oncotarget.com)
  • At present, the phenotypic heterogeneity and functional variation of human γδ T cells are increasing. (explorationpub.com)
  • In this review, we use the Heilig nomenclature to describe human γδ T cells [ 1 ]. (explorationpub.com)
  • BACKGROUND: Overexpression of PD-L1 is observed in many types of human cancer, including glioblastoma (GBM) and contributes to tumor immune evasion. (bvsalud.org)
  • MiR-146a-3p mimic and inhibitor were pre-transfected into RAW264.7 cells and the effects on M1 polarization induced by PM 2.5 were evaluated. (medsci.org)
  • This invention relates to methods of inducing differential stress resistance in a subject with cancer by starving the subject for a short term, administering a cell growth inhibitor to the subject, or reducing the caloric or glucose intake by the subject. (justia.com)
  • The method may further comprise administering to the subject a cell growth inhibitor. (justia.com)
  • Another method of the invention comprises administering a cell growth inhibitor to the subject and administering to the subject a chemotherapy agent. (justia.com)
  • This suggests that autophagy is a form of adaptation of the nutritional environment of rapidly growing tumor cells in response to hypoxia. (spandidos-publications.com)
  • NF-κB constitutive activation is found either in aggressive diffuse large B-cell lymphomas (DLBCL) with an activated phenotype (ABC-DLBCL), or in indolent B-cell lymphomas such as chronic lymphocytic leukemia, Waldenström Macroglobulinemia, marginal zone B-cell lymphomas (MZL) [ 4 ]. (springer.com)
  • Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
  • Increased survivin mRNA transcripts were observed in 17-AAG and geldanamycin-treated HT-29 and HONE-1 cancer cells. (biomedcentral.com)
  • Photodynamic therapy (PDT) is a treatment that involves photosensitizing agents and light to kill cancer cells. (spandidos-publications.com)
  • To study the effect of p62 on cancer cell death, we used PDT to treat a stable cell line overexpressing p62. (spandidos-publications.com)
  • Overexpression of p62 may contribute to colorectal cancer cell death. (spandidos-publications.com)
  • To treat patients with colorectal cancer, an approach that selectively targets cancer cells without damaging normal cells and which minimizes the risk of perforating the intestinal barrier is needed ( 2 ). (spandidos-publications.com)
  • MIF overexpression has been reported in several cancer types, including GBM. (nature.com)
  • Furthermore, cancer cells undergoing EMT phenotypic changes also acquire drug resistance [ 7 ]. (techscience.com)
  • A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
  • Until recently, the treatment of cancer has been largely focused on the development of therapeutic agents or techniques that kill cancer cells. (justia.com)
  • The present invention provides a novel approach to cancer therapy by providing a method to differentially enhance the resistance of normal cells to chemotherapeutic agents, thereby, improving the effectiveness of chemotherapeutic agents in killing cancerous cells. (justia.com)
  • In another aspect, the invention features methods of contacting a cancer cell with a chemotherapy agent and methods of increasing resistance of a non-cancer cell to a chemotherapy agent. (justia.com)
  • γδ T cells recruited into the tumor microenvironment can act as effector cells to mediate cancer immune surveillance. (explorationpub.com)
  • PURPOSE: Cutaneous melanoma is an aggressive and deadly cancer resulting from malignant transformation of cells involved in skin pigmentation. (bvsalud.org)
  • Transwell migration and invasion assays were done to evaluate changes in the ability of cell migration and invasion. (biomedcentral.com)
  • Chemokines are well-recognized for their ability to recruit different leukocytes (6) and for their involvement in the migration of circulating cells into or within tissue (7, 8). (researchsquare.com)
  • Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
  • Similar to αβ T cells, γδ T cells also develop in embryos, and their intra-thymic development shows strict regulation and flexible plasticity [ 2 ]. (explorationpub.com)
  • For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. (avivasysbio.com)
  • The induced differential stress resistance results in improved resistance to cytotoxicity in normal cells, which, in turn, reduces cytotoxic side-effects due to chemotherapy, as well as improved effectiveness of chemotherapeutic agents. (justia.com)
  • By making normal cells more resistant to chemotherapeutic agents, a patient's tolerance for cytotoxicity is improved, which, in turn, also improves the effectiveness of chemotherapy. (justia.com)
  • We previously showed that constitutive overexpression of the oncogenic transcription factor Spi1/PU.1 leads to pre-leukemic cells that have a shortened S phase duration with an increased replication fork speed and increased mutability in the absence of DNA breaks. (oncotarget.com)