Cell lines genetically. Medical search. Frequent questions

This in vitro study demonstrated that an encapsulated α-gal-secreting cell line can be used to treat Fabry mice by transplantation in vivo. (elsevierpure.com)
Robust F1 hybrid embryonic stem cell line from Mus musculus (lab mouse), generated by crossing C56BL/6J mice to Black-Swiss mice. (seattlechildrens.org)
NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
To this end, we currently use several (conditional) knock-out mice and cell lines to study gene expression patterns, metabolic profiles, protein interactions, intracellular trafficking, and signaling pathways. (nih.gov)
We study the mechanisms responsible for the tubes becoming cystic using genetically engineered mice that have PKD and cell lines that mimic some of the characteristics of PKD. (nih.gov)
By identifying a mechanism that regulates programmed cell death in precursor cells for blood, or hematopoietic stem cells, the work is the first to connect the death of such cells to a later susceptibility to tumors in mice. (scienceblog.com)
To study the role of ARTS, the experimenters bred a line of mice genetically engineered to lack the Sept4 gene. (scienceblog.com)
Newborn ARTS-deprived mice had about twice as many hematopoietic stem cells as their normal, ARTS-endowed peers, and those stem cells were extraordinary in their ability to survive experimentally induced mutations. (scienceblog.com)
The increase in the number of hematopoietic progenitor and stem cells in Sept4-deficient mice brings with it the possibility of accelerating the accumulation of mutations in stem cells," says Garcia-Fernandez. (scienceblog.com)
But what we have found is that biochemically and genetically, mice and humans aren't quite the same and don't behave the same. (medscape.com)

Its properties are well understood, and there are established techniques to genetically program many organisms to express GFP. (technovelgy.com)
However, deliberate or inadvertent releases of genetically engineered organisms into the environment could have negative ecological impacts under some circumstances"(Coker 24). (bartleby.com)
Transgenic plants and animals or genetically modified organisms (GMOs) that lack federal approval for release. (tamuk.edu)
Chimeras are organisms that contain two or more genetically distinct cell lines. (asu.edu)
The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)

Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
The proteins arethe "work-horses" of the cells and are responsible for carrying out all the functions of the cell. (faqs.org)
The researchers tested the mutant CARD11 proteins in other cell lines and found that the proteins spontaneously activated the NF-kB signaling pathway, thereby promoting the survival of malignant cells. (nih.gov)
Rational genetic engineering to enhance biotherapeutic proteins has become a reality catalyzed by publication of the genome sequences of multiple Chinese hamster ovary (CHO) cell lines. (bioprocessintl.com)
Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)
Cell Factory engineers contribute to the transition towards a sustainable society by supplying genetically engineered cell factories for fermentation-based production of proteins or biochemicals. (dtu.dk)
Research associate Maria Garcia-Fernandez, Hermann Steller, head of the Strang Laboratory of Apoptosis and Cancer Biology, and their colleagues explored the activity of a gene called Sept4, which encodes a protein, ARTS, that increases programmed cell death, or apoptosis, by antagonizing other proteins that prevent cell death. (scienceblog.com)
ARTS interferes with molecules called inhibitor of apoptosis proteins (IAPs), which prevent cells from killing themselves. (scienceblog.com)
We are interested how extracellular matrix proteins instruct cells to differentiate, migrate, and proliferate, and how cells sense normal and pathologically altered extracellular matrix. (mcgill.ca)
This complex targets other proteins to be broken down (degraded) by the cell when they are no longer needed. (medlineplus.gov)
Protein degradation is a normal process that removes damaged or unnecessary proteins and helps maintain the normal functions of cells. (medlineplus.gov)

Consistent with a central role of Wnt signaling in regulation of gene expression, inhibition of PORCN in RSPO3-translocated cancers causes a marked remodeling of the transcriptome, with loss of cell cycle, stem cell and proliferation genes, and an increase in differentiation markers. (nih.gov)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
When the genetic material within the living cells, i.e. genes are working properly, the human body can develop and function smoothly. (faqs.org)
Here, we used quantitative real-time PCR, profiling the expression of 93 genes in single-cells from three different cell lines. (frontiersin.org)
We found that the total transcript level per cell and the expression of most individual genes correlated with progression through the cell cycle, but not with cell size. (frontiersin.org)
Detailed analysis of cell cycle predictive genes allowed us to define subpopulations with distinct gene expression profiles and to calculate a cell cycle index that illustrates the transition of cells between cell cycle phases. (frontiersin.org)
In conclusion, we provide useful experimental approaches and bioinformatics to identify informative and predictive genes at the single-cell level, which opens up new means to describe and understand cell proliferation and subpopulation dynamics. (frontiersin.org)
Here, we employed single-cell gene expression profiling to describe the dynamic transition between cell proliferative states in three different cell lines using a panel consisting of 93 marker genes. (frontiersin.org)
Two separate research teams have figured out how to "reprogram" cells with just a handful of genes to give them the characteristics of embryonic stem cells. (nih.gov)
The egg then "reprograms" the adult nucleus so that the cell behaves like an embryo but has the genes of the adult cell. (nih.gov)
This includes some cell lines contaminated with bacteria or carrying genes and mutations associated with cancer. (neurosciencenews.com)
The cells were generated with a variety of genes, methods and cells of origin, such as adult skin or infant cord blood cells. (neurosciencenews.com)
The different methods included a variety of reprogramming genes, vectors (engineered viruses that deliver genetic material to cells), or the use of plasmids (small DNA molecules that can deliver reprogramming genes). (neurosciencenews.com)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
HIF controls several genes involved in cell division, the formation of new blood vessels, and the production of red blood cells. (medlineplus.gov)
The VHL protein likely plays a role in other cellular functions, including the regulation of other genes and control of cell division. (medlineplus.gov)

Researchers reported in Nature on November 22, 2007, that they successfully isolated 2 embryonic stem cell lines from cloned embryos made using cells from the skin of an adult rhesus macaque. (nih.gov)
The team that isolated the embryonic stem cell lines was led by Dr. Shoukhrat Mitalipov at Oregon Health and Science University in Portland. (nih.gov)
The stem cells, the researchers showed, could turn into heart or nerve cells in the laboratory, and had other characteristics of established embryonic stem cell lines. (nih.gov)
Background: Recent studies have associated the transcription factors, Oct4, Sox2 and Nanog as parts of a self-regulating network which is responsible for maintaining embryonic stem cell properties: self renewal and pluripotency. (lu.se)

The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
Not only did the cell-based device produce pulses of laser light as in the GFP solution experiment, the researchers also found that the spherical shape of the cell itself acted as a lens, refocusing the light and inducing emission of laser light at lower energy levels than required for the solution-based device. (technovelgy.com)
Modern researchers have conducted numerous studies on E. coli , and it is easier for us to manipulate genetically for productivity improvement, especially for large-scale production. (tamu.edu)
Researchers will screen 400 compounds on 1,000 lines of cancer cells derived from a variety of types of tumors. (technologyreview.com)
A new gene editing service provides researchers on campus with genetically engineered pluripotent stem cell lines derived using CRISPR-Cas9. (wisc.edu)
The researchers then genetically engineered AIEC bacteria to lack a key enzyme called propanediol dehydratase. (genengnews.com)
Before this new study was published, Nature asked another group of researchers to confirm that the stem cells were genetically identical to the donor skin cells. (nih.gov)
Previous studies by researchers at NIH's National Cancer Institute (NCI) showed that the malignant cells of the hard-to-treat ABC subtype rely on a series of molecular events involving a protein called CARD11. (nih.gov)
Hwang Woo-suk, a geneticist in South Korea, claimed in Science magazine in 2004 and 2005 that he and a team of researchers had for the first time cloned a human embryo and that they had derived eleven stem cell lines from it. (asu.edu)
Teratomas, benign tumors containing the developing cells of different body parts, allowed researchers to see if the iPSCs could form the body's three basic germ cell lines - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
The researchers also announced establishment of an on-line web portal and database hosted at https://www.synapse.org/ . (neurosciencenews.com)
The researchers compared 58 different lines of iPSCs submitted by the different institutions. (neurosciencenews.com)
Researchers also compared the molecular and functional characteristics of iPSCs to human embryonic stem cells, which are used sparingly as a gold standard for benchmarking quality. (neurosciencenews.com)

These have distinct advantages as they are naturally pluripotent, so can grow and divide indefinitely in cell culture in the right conditions, and differentiate into multiple different cell types, Toubia explains. (foodnavigator.com)

Genetic engineering does not include traditional animal and plant breeding, in vitro fertilization, induction of polyploidy, mutagenesis and cell fusion techniques that do not use recombinant nucleic acids or a genetically modified organism in the process. (bartleby.com)
Although this organism is genetically related to Cryptococcus neoformans , a cause of meningitis in HIV-infected persons, Cryptococcus gattii is frequently associated with a different spectrum of disease, prominently pneumonia. (cdc.gov)

Cancer cells acquire genetic heterogeneity to escape from immune surveillance during tumor evolution, but a systematic approach to distinguish driver from passenger mutations is lacking. (nature.com)
Genetic inactivation of the machinery or re-introduction of ANKRD52 frequent patient mutations dampens the JAK-STAT-interferon-γ signaling and antigen presentation in cancer cells, largely by abolishing miR-155-targeted silencing of suppressor of cytokine signaling 1 (SOCS1). (nature.com)
Because the TCA cycle is crucial for generating ATP, mutations that break the TCA cycle drastically reduce the ability of a cell to generate useful energy from blood nutrients. (mayo.edu)
Nonetheless, SDH mutations inherited from a parent set the stage for random and accidental loss of the remaining healthy SDH gene copy in somatic cells. (mayo.edu)
Now this genetic knowledge is being used to direct drug development by testing specific compounds on 1,000 cancer cell lines that incorporate many of these mutations. (technologyreview.com)
Collectively, the cells possess about 70 cancer-linked genetic mutations, about 50 to 80 percent of the mutations that have been uncovered to date. (technologyreview.com)
As they reported in the March 6, 2008, issue of Science , they discovered mutations in 3 of the biopsy specimens and 1 cell line. (nih.gov)
In the end, that leads to more cells accumulating mutations that cannot be eliminated. (scienceblog.com)
Some spontaneous mutations associated with this condition occur during the formation of reproductive cells (eggs or sperm) or just after fertilization and are called de novo mutations. (medlineplus.gov)
Other spontaneous mutations found in this condition, called somatic mutations, are acquired during a person's lifetime and are present only in the tumor cells. (medlineplus.gov)
At least 10 inherited mutations in the VHL gene have been found to cause familial erythrocytosis, a condition characterized by an increased number of red blood cells and an elevated risk of abnormal blood clots. (medlineplus.gov)

Inhibition of Wnt signaling by PORCN inhibition holds promise as differentiation therapy in genetically defined human cancers. (nih.gov)
We believe that for the full sensory properties of meats, you need to have some degree of cell differentiation so you get the unique chemical compounds which make the taste and the flavor of meat. (foodnavigator.com)

This microscopic image shows a cross section from a teratoma, generated in the lab by authors of a June 9 study in Stem Cell Reports that tested the quality of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
As the promise of using regenerative stem cell therapies draws closer, a consortium of biomedical scientists reports about 30 percent of induced pluripotent stem cells they analyzed from 10 research institutions were genetically unstable and not safe for clinical use. (neurosciencenews.com)
In a study published June 9 by the journal Stem Cell Reports , and funded by the National Heart, Lung, and Blood Institute, the multi-institutional research team reports on the comprehensive characterization of a large set of induced pluripotent stem cells (iPSCs). (neurosciencenews.com)
Being pluripotent means iPSCs can generate cells from each of the three basic germ cell lines that form the body - endoderm (gut region), ectoderm (epidermis, nerve tissue, etc.) and mesoderm (muscles, blood cells, etc. (neurosciencenews.com)
It had been thought that poor quality iPSC lines could not be pluripotent, Lutzko said, although until the current study this theory had not been carefully tested because of the expense. (neurosciencenews.com)

In another strategy, called therapeutic cloning, the embryo can instead be used to create stem cells that are genetically identical to a patient. (nih.gov)
In certain outbreaks, the detection of a genetically identical strain of NLV among patients from different geographic locations provided substantial evidence to support the link among the cases that was indicated by epidemiologic observations. (cdc.gov)
Tumors are not simply a compilation of molecularly and phenotypically identical neoplastic cells. (lu.se)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)

Enzyme replacement using IV doses of recombinant human α-gal produced in CHO cells or in human fibroblasts is currently being evaluated in clinical trials as a potential therapy for this disease. (elsevierpure.com)
The CHO cell line secretes recombinant factor IX into a defined cell culture medium that does not contain hormones, and the recombinant factor IX is purified by a chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)

1 Program in Cancer and Stem Cell Biology, Duke-NUS Graduate Medical School, Singapore, Singapore. (nih.gov)
Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)
Frontiers in Cell and Developmental Biology, 9 (12). (dcu.ie)
Focus areas include optimization of cellular performance using techniques and knowledge from synthetic biology, genetic engineering, secondary metabolites, cell physiology, and regulatory networks. (dtu.dk)
He also has professional interests in stem cell research, the biology of aging, cancer as well as telomere and telomerase biology. (stanford.edu)
Part of the Progenitor Cell Biology Consortium, the scientists are working to make sure this growing area of medical research is grounded in safe and sound science. (neurosciencenews.com)
Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
Every cell and organ in the body has either blood or fluids moving through it, so those principles are extremely important to restoring human tissue biology. (medscape.com)

The outcomes highlighted the importance of the gene of interest as a therapeutic target to investigate genetically unstable ovarian cancers to further elucidate its mechanisms of action. (medicalxpress.com)
Further applications of CRISPR are appearing at a furious pace, and gathering momentum toward therapeutic use in human cells. (bibalex.org)
ReNeuron has leveraged its stem cell technologies into non-therapeutic areas - its ReNcell® range of cell lines for use in research and in drug discovery applications in the pharmaceutical industry. (biospace.com)
And most recently, last month, we reported that Activiomics and Belgian biopharma UCB will apply Activiomics' Targeted In-depth Quantification of cell Signaling (TIQUAS) phospho-proteomics platform-a system that is quantitative, label-free and applicable to cell and tissue samples-in a collaborative effort to elucidate signaling mechanisms of therapeutic antibodies in relevant cell-based systems. (drugdiscoverynews.com)
Therapeutic monoclonal antibodies (MAbs) mostly are manufactured using bioengineered mammalian cells cultured in a bioreactor for two to three weeks. (bioprocessintl.com)
In 2012, he became a member of the Canary Center at Stanford University where he has leveraged his experience in cell and preclinical studies to develop imaging modalities to track therapeutic responses against cancer as well as detecting early stage cancers. (stanford.edu)
The identification of the ARTS gene and its role in cancer cell death provides a potential target for new therapeutic approaches. (scienceblog.com)
Cancer stem cells are a population of slow-cycling cells within the tumour bulk, with self-renewal capacity that attracts interest as a therapeutic target. (lu.se)

This copy can be obtained from a natural source (like DNA in islets of Langerhans cells), or it can be manufactured artificially in the laboratory. (faqs.org)
In the current study, NCI scientists and their colleagues examined the sequence of the CARD11 gene in 16 ABC DLBCL biopsy specimens and 4 ABC DLBCL laboratory cell lines. (nih.gov)
The scientists also discovered that they were able to kill ABC DLBCL cells in the laboratory by genetically interfering with the function of the CARD11 coiled-coil domain. (nih.gov)
The Reinhardt laboratory is interested in components and mechanisms involved in the biogenesis, function and pathological aspects of the extracellular matrix and its interactions with cells. (mcgill.ca)

9. Björklund, A., Skagerberg, G.: Evidence for a major spinal cord projection from the diencephalic A11 dopamine cell group in the rat. (lu.se)

B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
PKCa and PKCe activities were inhibited using isoform-specific pharmacologic inhibitors and genetically modified dominant-negative (DN) expressing cell lines. (cdc.gov)
By inhibiting these inhibitors, under the right circumstances ARTS helps to take the brakes off the process of apoptosis, permitting the cell to die on schedule. (scienceblog.com)
[ 2 ] Options for second-line therapy in patients with relapsed/refractory disease include chemotherapy-free regimens with biologic targeted agents such as covalent Bruton tyrosine kinase (BTK) inhibitors, lenalidomide,venetoclax, and chimeric antigen receptor (CAR) T-cell therapy. (medscape.com)

Label-free detection, on the other hand, is proving to be a highly sensitive method of measurement for endogenous targets in live cell assays, and eliminates the need for tags, dyes or specialized reagents or engineered cells-reducing the resources required for assay development, simplifying assay design, minimizing liabilities created by the use of labels and enabling the use of native cells for greater biological relevance. (drugdiscoverynews.com)
In November 2010, we brought you the news that ACEA Biosciences Inc. and Vivo Biosciences Inc. agreed to work together to develop label-free and real-time cell-based assays for the xCELLigence System, which is co-developed by Roche and ACEA and marketed by Roche Applied Science. (drugdiscoverynews.com)
Furthermore, because of the antigenic and genetic diversity of 'Norwalk-like viruses'* (NLVs) and the inability to cultivate these viruses in cell lines, developing assays to detect the full spectrum of NLVs associated with outbreaks of gastroenteritis was not possible. (cdc.gov)
We evaluated cell stemness by using multiple functional assays in combination with stem cell marker expression analysis. (lu.se)

Scientists at various institutions are also researching its use in human germ cells, including eggs, sperm, and embryos, which could confer major benefits. (bibalex.org)
This ES cell line does not require feeder cells for propagation and readily transmits through the germline using outbred CD1 4-8 cell embryos as hosts. (seattlechildrens.org)

It is applying its novel stem cell platform technologies in the development of ground-breaking stem cell therapies to serve significant and unmet or poorly-met clinical needs. (biospace.com)
In addition to its stroke programme, ReNeuron is developing stem cell therapies for Parkinson's disease, Huntington's disease, Type 1 diabetes and diseases of the retina. (biospace.com)
Advancing CAR T therapies for patients across lymphomas remains a cornerstone of our cell therapy development program, and we are excited about the potential of Yescarta for patients with indolent follicular lymphoma. (gilead.com)
It was very surprising to us the high number of unstable cell lines identified in the study, which highlights the importance of setting safety standards for stem cell therapies," said Carolyn Lutzko, PhD, senior author and director of translational development in the Translational Core Laboratories at Cincinnati Children's Hospital Medical Center. (neurosciencenews.com)
A good number of the cell lines we studied met quality standards, although the unexpected number of lines that did not meet these standards could not be used for clinical therapies. (neurosciencenews.com)
It gives scientists open access to data from the study to support their own research into potential iPSC-based stem cell therapies. (neurosciencenews.com)

Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
Expression of each miRNA machinery component strongly correlates with intratumoral T cell infiltration in nearly all human cancer types. (nature.com)
The possibility of successfully creating a genetically-modified human is no longer science fiction. (bibalex.org)
Indeed, Chinese scientists recently began a human clinical trial using CRISPR-edited cells to fight lung cancer, and US clinical trials will begin this year. (bibalex.org)
The main events of human meiosis during egg cell maturation. (scienceinschool.org)
A: During the first five months of development of a female human embryo, all its potential future egg cells are formed. (scienceinschool.org)
Other human cells have also been genetically engineered to create SDH deficiency. (mayo.edu)
SANTA MONICA, Calif.--(BUSINESS WIRE)-- Kite, a Gilead Company (Nasdaq: GILD), today announces that the European Medicines Agency (EMA) Committee for Medicinal Products for Human Use (CHMP) has issued a positive opinion for Yescarta ® (axicabtagene ciloleucel) for adult patients with diffuse large B-cell lymphoma (DLBCL) and high-grade B-cell lymphoma (HGBL) that relapses within 12 months from completion of, or is refractory to, first-line chemoimmunotherapy. (gilead.com)
The UW-Madison iPSC Reprogramming and Human Stem Cell Gene Editing Service is co-operated by the Waisman Center's iPSC Core and led by investigators Anita Bhattacharyya and Su-Chun Zhang. (wisc.edu)
Our goal is to provide the technology - we make the human stem cell lines that people want - so that the labs can focus on addressing the underlying scientific questions," says Bhattacharyya. (wisc.edu)
In particular, the efficiency of the process will have to be improved before the technique could be applied in the clinic using human cells. (nih.gov)
Dust samples collected from Nebraska swine confinement facilities (hog dust extract [HDE]) are known to elicit proinflammatory cytokine release from human bronchial epithelial (HBE) cells in vitro. (cdc.gov)
Robert describes part-human animals, otherwise known as chimeras, as those resulting from the intentional combination of human and nonhuman cells, tissues, or organs at any stage of development. (asu.edu)
Stem cells have an enormous amount of potential to increase the health, and overall living standards of the human species in general. (scienceblog.com)
Specialized iPSCs are reprogrammed from adult skin or infant cord blood cells and can become any cell type in the body - a condition called pluripotency that mimics the function of human embryonic stem cells (hESCs). (neurosciencenews.com)
Here we investigated the characteristics of a population of pancreatic cancer tumorspheres derived from different human pancreatic cancer cell lines and a primary line from a genetically engineered KPC mouse model, using flow cytometry and western blotting to analyse surface and stemness markers. (lu.se)
Genetically modified L20B (Mouse) and RD (Human Rhabdomyosarcoma) cell lines are commonly used in poliovirus research, surveillance, and diagnostics. (cdc.gov)
We evaluated specificity of the assay with five human and four mouse cell lines, as well as monkey and rat cell lines. (cdc.gov)
The assay showed no cross-reactivity with genomic DNA from human, mouse, rat, or monkey cell lines. (cdc.gov)

In this review, we outline the occurrence and role of DDR deficiency in PDAC and provide an overview of clinical trials that target these pathways and the preclinical models such as 2D cell lines, 3D organoids and mouse models [genetically engineered mouse model (GEMM), and patient-derived xenograft (PDX)] used in PDAC DDR deficiency research. (dcu.ie)

Using that information, their next step was to develop a line of mammalian cells expressing GFP at the required levels. (technovelgy.com)

Namely, the technology could eradicate hereditary diseases, such as cystic fibrosis, sickle-cell anemia, and Huntington's disease from a family line altogether. (bibalex.org)

It also is vital that - prior to being instructed to become a specific cell type - iPSC lines continuously renew and expand in a blank slate form without the introduction of genetic errors. (neurosciencenews.com)
How well the 58 iPSC lines met quality criteria depended on the origin of the reprogrammed cells (skin vs. blood, male vs. female) and specific reprogramming methods. (neurosciencenews.com)
Pluripotency can be tested to determine if iPSC lines are able to form what are called teratomas - benign tumors made up of different cell types (teeth, bone, brain, etc. (neurosciencenews.com)
This prompted Lutzko and her colleagues to check and see whether all iPSC lines - both high and low quality - could generate teratomas. (neurosciencenews.com)

In 2005 laws were passed giving scientists approval to carry out experiments that could lead to the first genetically altered babies being born in Britain, showing how close we are to genetically modified humans. (bartleby.com)

In 2002, he joined Stanford University where he initially utilized his experience from Geron Corporation to research cardiovascular and endothelial progenitor cell function. (stanford.edu)
In mature B and T cells, she could not find any, however, so she began to look at cells earlier and earlier in development, until finally she was comparing hematopoietic progenitor and stem cells. (scienceblog.com)
This work not only defines the role of the ARTS gene in the underlying mechanism of mammalian tumor cell resistance to programmed cell death, but also links this gene to another hallmark of cancer, stem and progenitor cell proliferation," said Marion Zatz, who oversees cell death grants, including Steller's, at the NIH's National Institute of General Medical Sciences. (scienceblog.com)

Insome cases, a single misspelled base is sufficient to cause a disease, suchas sickle cell anemia. (faqs.org)

E) Confocal microscopic images of Cy5siRNA-labeled LNP internalization in SKOV3, NAR, and A549 cells. (medicalxpress.com)
These chips reveal fungal cells for microscopic analysis. (lu.se)

Cell Signal (2020 Oct) 74:109701. (nih.gov)

In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)
Mantle cell lymphoma (MCL) is recognized in the Revised European-American Lymphoma and World Health Organization classifications as a distinct clinicopathologic entity. (medscape.com)

Their experiments revealed that a metabolite produced by the bacteria interacts with immune system cells in the lining of the intestine, triggering inflammation. (genengnews.com)

In highly heterogeneous tumours, like pancreatic ductal adenocarcinoma (PDAC) however, the characterisation of cancer stem cells has led to controversial results due to the lack of consensus on specific markers. (lu.se)

Those whose cancer returns or has already spread beyond the prostate, are treated with hormone therapy that reduces levels of the male sex hormones that prostate cells need in order to survive. (wikipedia.org)
Guildford, UK, 1 February 2008: ReNeuron Group plc (LSE: RENE.L) today provides an update on progress with its Investigational New Drug (IND) application to the US Food and Drug Administration (FDA) to commence initial clinical trials in the US with its ReN001 stem cell therapy for stroke. (biospace.com)
ReNeuron is a leading, UK-based stem cell therapy business. (biospace.com)
ReNeuron has filed for approval to commence initial clinical studies in the US with its lead ReN001 stem cell therapy for chronic stroke disability. (biospace.com)
If approved, Yescarta will be the first Chimeric Antigen Receptor (CAR) T-cell therapy approved for patients in Europe who do not respond to first-line treatment. (gilead.com)
At Kite, we are committed to bringing the curative potential of cell therapy to the world, and changing the way cancer is treated," said Christi Shaw, CEO, Kite. (gilead.com)
Today's positive CHMP opinion brings us a step closer to utilizing cell therapy earlier in the treatment journey, potentially transforming the standard of care for the most common and aggressive form of non-Hodgkin lymphoma. (gilead.com)
The positive opinion for Yescarta is based on the primary results of the landmark Phase 3 ZUMA-7 study, the largest and longest trial of a CAR T-cell therapy versus standard of care (SOC) in second-line LBCL. (gilead.com)
Inside "the moral evaluation of germ line cell therapy is different. (bartleby.com)
SANTA MONICA, Calif.--(BUSINESS WIRE)-- Kite, a Gilead Company (Nasdaq: GILD), today announced that the U.S. Food and Drug Administration (FDA) has granted accelerated approval to Yescarta ® (axicabtagene ciloleucel) for the treatment of adult patients with relapsed or refractory follicular lymphoma (FL) after two or more lines of systemic therapy. (gilead.com)
The approval makes Yescarta the first chimeric antigen receptor (CAR) T-cell therapy approved for patients with indolent follicular lymphoma, follows FDA Breakthrough Therapy Designation and a priority review, and marks the third approved indication for a Kite cell therapy. (gilead.com)
Once a follicular lymphoma patient's disease relapses, the duration of response to care shortens with each round of therapy," said Caron A. Jacobson, MD, MMSc, Medical Director, Immune Effector Cell Therapy Program, Dana-Farber Cancer Institute and Assistant Professor of Medicine, Harvard Medical School. (gilead.com)
Additionally, for follicular patients in the third line of therapy, the five-year survival rate is only 20 percent, highlighting the urgent need for treatments that offer a real chance for durable remission. (gilead.com)
Currently, there are limited options for the treatment of relapsed or refractory indolent FL after two or more lines of therapy. (gilead.com)
ZUMA-5 is an ongoing, single-arm, open-label, multicenter trial evaluating 146 patients (≥18 years old) with relapsed or refractory iNHL, who received at least two prior lines of systemic therapy, including the combination of an anti-CD20 monoclonal antibody and an alkylating agent. (gilead.com)

ReNeuron has used its c-mycER technology to generate genetically stable neural stem cell lines. (biospace.com)

Prostate cancer is the uncontrolled growth of cells in the prostate, a gland in the male reproductive system just below the bladder. (wikipedia.org)
This halts tumor growth for a while, but eventually cancer cells grow resistant to this treatment. (wikipedia.org)
Dose-dependent response of CKAP5 knockdown across various cancer cell lines. (medicalxpress.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)
We find that the core microRNA (miRNA) biogenesis and targeting machinery maintains the sensitivity of cancer cells to PD-1-independent T cell-mediated cytotoxicity. (nature.com)
Our data indicate that the evolutionarily conserved miRNA pathway can be exploited by cancer cells to escape from T cell-mediated elimination and immunotherapy. (nature.com)
Cancer immunotherapies such as immune checkpoint blockade (ICB) unleash T cell cytotoxicity against cancer cells and have significantly improved the perspective of cancer patients. (nature.com)
Two complementary approaches have been adopted to identify genetic elements that control cancer cell sensitivity to T cell-mediated cytotoxicity. (nature.com)
The other approach is to screen CRISPR-Cas9-based guide RNA (gRNA) libraries that target either the whole genome using cancer cell and immune cell co-culture systems 9 , 10 , or focused gene sets using immunocompetent murine tumors 11 , 12 . (nature.com)
Combined, these approaches have led to the discovery of a myriad of mechanisms underlying cancer susceptibility to or evasion from T cell attack. (nature.com)
One line of cancer cells is shown here. (technologyreview.com)
Previous efforts to screen new drugs on cancer cell lines have focused on just tens of different lines. (technologyreview.com)
The compounds to be screened in the Mass General/Wellcome study include both traditional chemotherapies, which can affect both healthy and cancerous tissue, and molecularly targeted drugs, designed to attack a particular genetic flaw specific to cancer cells. (technologyreview.com)
Diffuse large B-cell lymphoma (DLBCL), a cancer that originates in white blood cells called B lymphocytes, is the most common form of non-Hodgkin lymphoma, accounting for 30-40% of cases. (nih.gov)
Now research from Rockefeller University shows that having too many stem cells, or stem cells that live for too long, can increase the odds of developing cancer. (scienceblog.com)
We make a connection between apoptosis, stem cells and cancer that has not been made in this way before: this pathway is critically important in stem cell death and in reducing tumor risk," Steller says. (scienceblog.com)
The work supports the idea that the stem cell is the seed of the tumor and that the transition from a normal stem cell to a cancer stem cell involves increased resistance to apoptosis. (scienceblog.com)
It also suggests that the premature silencing of the Sept4/ARTS pathway at the stem cell level may herald cancer to come. (scienceblog.com)
Moreover, the cancer cells themselves are highly plastic, with some of the cells exhibiting stem-like phenotypes. (lu.se)

The economics of this approach may be more attractive than tissue engineering in the short term, says the team at Aleph Farms, which is co-culturing multiple bovine cell types around sheets of vegetable-based, porous sponge-like scaffolding to make thin strips of tissue containing muscle, fat, and extracellular matrix (collagen and other components). (foodnavigator.com)
Plant cells are surrounded by an extracellular matrix that consists mainly of polysaccharides. (mdpi.com)
The dynamics of cell-extracellular matrix interactions in normal and pathological situations. (mcgill.ca)
Astrocytes became reactive in response to these cues and produced extracellular matrix that altered glioma cell properties, including stemness. (lu.se)
The VHL protein is also involved in the formation of the extracellular matrix, which is an intricate lattice that forms in the spaces between cells and provides structural support to tissues. (medlineplus.gov)

In each of these cells, after DNA duplication, homologous chromosomes exchange genetic material during crossing over. (scienceinschool.org)
The genetic material of the polar bodies is discarded, while that of the mature egg cell is joined by the genetic material of the fertilising sperm, to start the development of a new embryo. (scienceinschool.org)
Genetic engineering is the altering of the genetic material of living cells in order to make them capable of producing new substances or performing new functions. (faqs.org)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)

Cell growth can therefore be completely arrested prior to in vivo implantation. (biospace.com)

A single cell genetically engineered to express green fluorescent protein (GFP) can amplify photons into nanosecond-long pulses of laser light. (technovelgy.com)
While Aleph Farms is deploying tissue engineering techniques pioneered in regenerative medicine to make beef steaks, many other startups - under pressure from investors to get a minimum viable product (nuggets, burgers) out in front of consumers - are simply harvesting cell biomass (a slurry of cells that have not yet differentiated and matured into tissue) and adding extruded vegetable protein to create a meaty texture. (foodnavigator.com)
If the recipes have extra bases or misspelled bases or if some are deleted, the cell can make a wrong protein or too much or too little of the right one. (faqs.org)
Eventually, scientists aim to sequence the entire protein-coding region of all the cell lines, enabling even more comprehensive analysis. (technologyreview.com)
We have demonstrated proof of concept by creating a general plasma membrane bound decoy receptor, by displaying a protein or a peptide genetically fused to a trunctated version of the CD40 molecule. (lu.se)
When this decoy receptor is engaged by a ligand to the displayed protein/peptide, the receptor expressing cell is rescued from apoptosis. (lu.se)
Based on this function, the VHL protein is classified as a tumor suppressor, which means it prevents cells from growing and dividing too rapidly or in an uncontrolled way. (medlineplus.gov)
As in von Hippel-Lindau syndrome, when the VHL protein is altered, the HIF-2α protein is not broken down, and instead builds up in cells. (medlineplus.gov)

For people with DLBCL and HGBL who do not respond to first-line treatment or have an early relapse, outcomes are often poor and there are limited curative treatment options for these patients," said Marie José Kersten, Professor of Hematology at Amsterdam University Medical Centers, Amsterdam. (gilead.com)

to repair damage at that point, scientists can change or add DNA within the cell. (bibalex.org)
And, because the microscope was only firing light at that small region of the oocyte, it did less damage to the cell, which enabled the scientists to keep up the imaging for the 10 hours of cell division (see box for more on smart microscopy ). (scienceinschool.org)
There comes a point when scientists cross a line and that is when they start to create designer babies. (bartleby.com)

This is an important new treatment option for people with third-line relapsed or refractory follicular lymphoma," said Lee Greenberger, PhD, Chief Scientific Officer of The Leukemia & Lymphoma Society (LLS). (gilead.com)

The major impact of CRISPR has been in developing new model systems, cells, and animals, that are more rapid to develop and much more accurate than previous genetic models," Dr. Ed Wild, from UCL Institute of Neurology said. (bibalex.org)

Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
Ultimately, it will also help me in establishing a new line of research which will 'bring to the table' advanced microspectroscopy tools for studying single-cell scale soil microbial chemistry. (lu.se)

METHODS: This assay targets the mitochondrial cytochrome c oxidase subunit I (MTCO1) gene, a highly conserved and emergent DNA barcode region for detection of cross-contamination in RD and L20B cell lines. (cdc.gov)
RESULTS: The newly developed assay is very sensitive with a limit of detection of 100 RD cells/1 million L20B/mL. (cdc.gov)
CONCLUSIONS: We have developed and validated a TaqMan real-time PCR assay, a sensitive method for the detection of cross-contamination of RD and L20B cell lines. (cdc.gov)

The techniques and expertise that keep the service running at maximum capacity have driven demand for their stem cell lines so high that the project may need to expand, explains Bhattacharyya. (wisc.edu)

However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)

Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)
Several lines of evidence suggest that they function as a receptor-channel complex, but what they sense and signal is poorly understood. (nih.gov)
To design a high-throughput system with a highly parallel capacity, we utilized the B cell line WEHI-231, as carrier of the decoy receptor. (lu.se)
One specific peptide-displaying cell could be identified and amplified, based on a specific receptor engagement, in a background of 12 500 wild-type cells after four selections. (lu.se)

By examining mouse egg cells under the microscope, EMBL scientist Tomoya (Tomo) Kitajima was the first to track the movements of all of an egg cell's kinetochores during the whole of cell division - all 10 hours of it. (scienceinschool.org)
Using optogenetically guided recordings in mouse thalamocortical slices, we achieved the first reported paired intracellular recordings from nine monosynaptically connected thalamic and cortical neurons, including principal cells and two subtypes of inhibitory interneurons, and compared between cortical responses to single thalamocortical spikes and bursts. (jneurosci.org)
For this work, we used genetically engineered mouse models of glioma, primary stromal and glioma cell lines, classical glioblastoma cell lines, and organotypic slice cultures. (lu.se)

Suppose, for example, that one wishes to insert the insulin gene into a bacterial cell. (faqs.org)
Cell cultures derived from mammalian and bacterial cell lines are the conventional production systems in bioprocessing. (bioprocessintl.com)
Within this study line, you will obtain competences in designing, constructing, and optimizing cell factories using either bacterial, yeast, molds, plant or animal cell cultures. (dtu.dk)

The analytical sensitivity was also evaluated by spiking varying amounts of RD cells (0.001% - 10%) into L20B cells. (cdc.gov)

Cell proliferation includes a series of events that is tightly regulated by several checkpoints and layers of control mechanisms. (frontiersin.org)

Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)

A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)
These tools allow Yeng to test hypotheses about how loss of SDH creates an imbalance of metabolite molecules that confuses neuroendocrine cells into growing out of control. (mayo.edu)

Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
By increasing excitation of principal cells together with SOM-mediated, distally directed inhibition, thalamocortical bursts could momentarily enhance the saliency of the ascending sensory stimulus over less urgent, top-down inputs. (jneurosci.org)

Cloning entails taking the nucleus - the compartment that contains the DNA - from an adult cell and putting it into an egg from which the original nucleus has been removed. (nih.gov)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)

Antibodies One of the body's lines of defense ( immune system) involves white blood cells (leukocytes) that travel through the bloodstream and into tissues, searching for and attacking microorganisms and. (msdmanuals.com)

it was frequently categorized as diffuse small-cleaved cell lymphoma (by the International Working Formulation) or centrocytic lymphoma (by the Kiel classification). (medscape.com)

However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)

Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
Propionate was observed to interact with a type of immune system cell called mononuclear phagocytes that are also found in the lining of the gut and lead to inflammation. (genengnews.com)

Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)

The cellular laser was assembled by placing a single GFP-expressing cell -- with a diameter of from 15 to 20 millionths of a meter -- in a microcavity consisting of two highly reflective mirrors spaced 20 millionths of a meter apart. (technovelgy.com)
Critics also argue that labeled technologies typically require the use of genetically modified cell lines that can alter cellular behavior. (drugdiscoverynews.com)

and why only neuroendocrine cells are at risk for tumorigenesis. (mayo.edu)

We addressed how treatments and hypoxia affect tumor-associated astrocytes in ways that consequently alter glioma cell properties, and how hypoxia and pseudo-hypoxia are involved in stemness maintenance in glioblastoma. (lu.se)
We showed that the generation of the cleaved form of the cell surface glycoprotein CD44 leads to the stabilization of the HIFs in the perivascular and the perinecrotic glioma niche, leading to increased hypoxic signaling and glioma cell stemness. (lu.se)
Moreover, we showed that p75 NTR signaling is involved in the activation of the hypoxic signaling pathway and is also regulating glioma cell stemness and migration in hypoxia. (lu.se)

Anatomy34

Organisms12

Diseases36

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes64

Disciplines and Occupations1

Information Science3

Mice10

Organisms5

Proteins11

Genes18

Embryonic stem4

Researchers13

Differentiate1

Organism2

Mutations11

Differentiation2

Pluripotent5

Identical5

Recombinant2

Biology10

Therapeutic8

Laboratory4

Dopamine1

Inhibitors4

Assays4

Embryos2

Therapies6

Human20

Preclinical1

Mammalian cells1

Cystic1

IPSC4

Humans1

Progenitor3

Sickle-cell a1

Microscopic2

20201

Distinct2

Bacteria1

Pancreatic1

Therapy15

Generate1

Cancer21

Extracellular matrix5

Genetic material4

Vivo1

Protein8

DLBCL1

Scientists3

Relapsed or refractory1

CRISPR1

Microbial2

Detection3

Stem cell lines1

Donor1

Receptor4

Mouse3

Bacterial3

Analytical1

Mechanisms1

Endoderm1

Molecules2

Inhibition2

Nucleus2

Body's1

Diffuse1

Malignant1

Immune3

Somatic1

Cellular2

Tumorigenesis1

Stemness3