Cell lines for authentication. Medical search. Frequent questions

However, research results can be altered by contamination of cell lines by other human and non-human sources, such as the mouse feeder cells that are part of the culture environment, or bacteria such as mycoplasma. (dnacenter.com)
Mycoplasma testing of all cell cultures. (iclac.org)
Be a part of the movement to raise credibility in science and order your cell line authentication and mycoplasma testing services today. (atcc.org)
In-depth characterization analysis of Penl1 cell line revealed tumorigenicity in immunodeficient mice, negative human papilloma virus (HPV) and mycoplasma infection, TP53 mutations and sensitivity to cisplatin and epirubicin. (oncotarget.com)
This cell has passed the mycoplasma test. (ubigene.us)
Deliver 2 clonally expanded mycoplasma free iPSC lines (15 x 300k cells). (lu.se)

Because of the invaluable nature and numerous applications of cell lines in life science research, it is critical that they are handled carefully to not only avoid contamination but to protect human safety. (atcc.org)
This indicates that at some point in the past, one of these cell lines became misidentified, meaning that it ceased to correspond to the reported donor and instead became falsely identified (through cross-contamination or other means) as a cell line from a different donor. (providence.org)
Furthermore, DNA fingerprinting of samples from 5 different passage numbers of the same cell line showed excellent reproducibility when FLA was performed with the Bioanalyzer, indicating that no cross-contamination had occurred during the culture period. (port.ac.uk)
The authenticity and certification of cell lines used in biomedical research is very important to ensure reliable results and to avoid misidentification and cross-contamination among the cells. (fapesp.br)
The analysis of isoenzyme polymorphism is the standard method in quality control of cell line identification and interspecies contamination and is routinely use by main Biological Resource Centers. (fapesp.br)
On the other hand, the short tandem repeat profiling have been developed to authenticate human cell lines detecting intraspecies contamination too. (fapesp.br)
Any cell lines established via this solicitation must be fully confirmed through a rigorous and validated authentication and be contamination-free. (sbir.gov)
These comprise basic cell culture technology, e.g. cell cultivation and expanding cell cultures, but also authentication and contamination tests. (dsmz.de)
Evidence suggests that up to a third of established tumor cell lines being used for research are affected by inter- or intra-species cross-contamination or have been wrongly identified. (labmanager.com)
ESCC cell line CSEC216 from high incidence region in China was established with no cross-contamination. (biomedcentral.com)
Frequent bacterial, viral and inter-species contaminations can be rapidly detected by Multiplexion´s Multiplex Cell Contamination Test . (multiplexion.de)
Contamination of one human cell line by another human cell line (intra-human contamination) and human cell line authentication is monitored by Multiplexion´s Multiplex Cell Line Authentication , which is based on Single Nucleotide Polymorphism (SNP) typing. (multiplexion.de)
We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)

At this point, the creator of the cell line must perform some form of authentication and characterization that identifies it as a unique cell line. (atcc.org)
Dr. Multani has extensive expertise in the field of Human and Mammalian Cytogenetics, and specializes in the evaluation of genetic instability and complex chromosomal rearrangements in cancer cells, telomere dysfunction, characterization of embryonic stem cells, and authentication of cell lines. (mdanderson.org)
basic characterization, including cell authentication (STR), undifferentiated state (FACS) and molecular karyotyping. (lu.se)

Cancer occurs when a somatic cell which normally cannot divide undergoes mutations which cause de-regulation of the normal cell cycle controls leading to uncontrolled proliferation. (wikipedia.org)
Multiple myeloma (MM) is characterized by the uncontrolled proliferation of malignant plasma cells in bone marrow. (aacrjournals.org)
Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
The introduction of cell culture techniques has helped a lot in understanding the physiological processes like cell signalling, neurobiology, cell proliferation, pathogenesis of diseases, apoptosis and even more. (benthamscience.com)
regulating the differentiation, proliferation, maintenance and sculpturing organs and tissues, functioning of immune system and the elimination of defective harmful cells. (benthamscience.com)
Activation of EGFR regulates essential cellular processes including cell migration, proliferation, and apoptosis ( 1 ). (biorxiv.org)
Cell Counting Kit‑8 assay and flow cytometry were undertaken to measure proliferation and apoptosis. (spandidos-publications.com)
shlncRNA XIST attenuated cell proliferation, invasion and migration, while increasing the apoptosis of hepatic carcinoma cells. (spandidos-publications.com)
The miR‑320a mimic and PIK3CA inhibitor could recover the effect of oe‑lncRNA in terms of the proliferation, invasion, migration and apoptosis of hepatic carcinoma cells. (spandidos-publications.com)
Long non-coding (lnc)RNAs play important roles in the development, differentiation, proliferation and apoptosis of cells ( 6 , 7 ). (spandidos-publications.com)
Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)

Recent studies have demonstrated that pharmacological inhibition of the Notch pathway with γ-secretase inhibitor (GSI) induces apoptosis of multiple myeloma (MM) cells via upregulation of the proapoptotic protein Noxa. (aacrjournals.org)
ABT-737, a novel BH3 mimetic, was shown to block Bcl-2 and Bcl-xL and induce MM cell apoptosis. (aacrjournals.org)
Signaling through the receptor/transcriptional regulator Notch plays an important role in the survival of tumor cells and their protection from apoptosis induced by chemotherapeutic agents. (aacrjournals.org)
The two events, while occurring simultaneously but independently, likely provide a disadvantageous situation in which the newly emerging mesenchymal cell population is more likely to be resistant to apoptosis than the epithelial cell population from which the mesenchymal cell population stemmed. (molvis.org)

Most freshly isolated, primary cell cultures undergo senescence, the process by which cells stop dividing after undergoing a certain number of cell divisions. (atcc.org)
Mycoplasmas frequently contaminate cell cultures. (atcc.org)
With over 95 years of expertise with cell and microbial cultures, ATCC has acquired and developed a vast body of best practices to aid researchers at all levels of proficiency to maximize the return on their biomaterials investment. (atcc.org)
For example, supposed thyroid lines were actually melanoma cells, supposed prostate tissue was actually bladder cancer, and supposed normal uterine cultures were actually breast cancer. (wikipedia.org)
The Cell Culture arm of this module provides technical expertise to investigators to assist in the establishment of primary cultures and development of new immortalized cell lines. (utsouthwestern.edu)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
Possible reasons are mostly mishandling of cells during routine culture and cryopreservation but also the lack of convenient tests for the quality control of cell cultures. (multiplexion.de)
There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)

One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
Cell invasion and migration were detected by Transwell assays. (spandidos-publications.com)
4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)

DDC's associate laboratory director, Dr. Thomas Reid, will be presenting a scientific poster detailing the techniques used in DDC's cell line authentication service this weekend at the American Society of Cell Biology (ASCB) Annual Meeting in Denver, Colorado. (dnacenter.com)
HuLu Directory .com / Science and Technology / Biology / Cell Line Authentication DNA Test - Detect Misidentification & Other Errors Early! (huludirectory.com)
Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. (wikipedia.org)
Immortalised cell lines are widely used as a simple model for more complex biological systems, for example for the analysis of the biochemistry and cell biology of mammalian (including human) cells. (wikipedia.org)
This simplifies analysis of the biology of cells which may otherwise have a limited lifetime. (wikipedia.org)
This can alter the biology of the cell and must be taken into consideration in any analysis. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)

Discover how to thaw cells and maintain viability. (atcc.org)
Due to their reduced cell density, dead cells can be removed to optimize viability before delivery. (labmanager.com)
These findings support the development of a potentially relevant therapeutic strategy to undermine the progression of normal cells to the mesenchymal phenotype in the naturally hypoxic lens without subverting cell viability. (molvis.org)

DDC's laboratory offers a convenient and affordable service to enable researchers to meet these cell line authentication requirements. (dnacenter.com)
The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. (utsouthwestern.edu)
With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. (ubigene.us)
After sending data to one of former Mizmedi researchers, we learned the stem cells were actually made in Mizmedi. (blogspot.com)
Presumably, the stem cells were changed in their nascent stage with those of Mizmedi because a total of six related researchers do not have any doubts as to the authenticity of stem cells. (blogspot.com)
Despite the varied causes of irreproducibility from factors that are difficult to control, cell line authentication (CLA) is a relatively simple operation that assures that the cells researchers believe they are working with are actually those cells, and that they are contaminant-free. (labmanager.com)
Sustainable and uncontaminated cancer cell lines provide available resources for researchers to utilize. (biomedcentral.com)

Signaling through the receptor/transcriptional regulator Notch plays an important role in tumor cell survival. (aacrjournals.org)
The karyotype demonstrated tumor cell patterns with aneuploidy and complex chromosomal aberrations. (biomedcentral.com)

One cell line named Penl1 was isolated from a lymph node metastasis (LNM) of penile squamous cell carcinoma (PeSCC), usual type and comprehensively characterized here. (oncotarget.com)
Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. (biomedcentral.com)
There are abundant esophageal squamous cell carcinoma (ESCC) cell lines around the world. (biomedcentral.com)

Cell Line Authentication DNA Test - Detect Misidentification & Other Errors Early! (huludirectory.com)
Authentication of M14 melanoma cell line proves misidentification of M" by Christopher Korch, Erin M Hall et al. (providence.org)
Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line. (providence.org)

CSEC216 was an uncontaminated cell line, exhibited epithelial cell features with polygonal morphology and adherent growth as monolayer. (biomedcentral.com)
cell authentication (STR), pluripotency and undifferentiated state confirmation, molecular karyotype, g-banding and iPS growth pattern and morphology analysis. (lu.se)

Choose your notification preferences (Email or SMS) for multi-factor authentication (MFA) and verify your identity by entering your password and a unique number you receive through your preferred contact method. (cdc.gov)

Our staff even took a flight to get a somatic cell because one of the five patients was a foreigner. (blogspot.com)
The fact adds suspicions that genders of somatic cell contributors correspond with those of Mizmedi stem cells. (blogspot.com)

The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)

This set of Institutional Best Laboratory Practices is designed for the authentication of cell lines and tissue samples used in biomedical research. (iclac.org)
The Institutional Best Laboratory Practices for Cell Line and Tissue Sample Authentication was written by Christopher T Korch and Rebecca E Schweppe, University of Colorado - Anschutz Medical Campus. (iclac.org)
This essential laboratory process covers the thaw, growth, propagation, and cryopreservation of cells that have been isolated from a donor organism. (atcc.org)
The founding laboratory should also institute the seed stock principle to bank the cells. (atcc.org)
HeLa, the first immortal human cell line on record to be successfully isolated and proliferated by a laboratory, was taken from Henrietta Lacks (without informed consent) in 1951 at Johns Hopkins Hospital in Baltimore, Maryland. (wikipedia.org)
Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
The aim of this work is the deployment in our laboratory of one molecular technique (mtDNA) for species identification of animal cell lines besides an improvement of the electrophoretic analysis of isoenzymes which will be used as control and pattern. (fapesp.br)
Our laboratory intends to perform and to available this valuable tool of the authentication of the cell lines. (fapesp.br)
Cell lines from various tumor types have been developed for use as a tumor model for controlled laboratory studies. (biomedcentral.com)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)

Best practices for reviving cells from cryopreservation are critical to ensuring unambiguous experimental results in basic research and industrial processes. (atcc.org)
These best practices form the basis of several cell culture guides , webinars , and video tutorials that detail the initiation, expansion, and cryopreservation of continuous cell lines, primary cells, hTERT-immortalized primary cells, and organoids. (atcc.org)

The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. (biomedcentral.com)
Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells. (biomedcentral.com)
The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. (biomedcentral.com)
Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. (biomedcentral.com)
Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells. (biomedcentral.com)
Its lethality is a consequence of the lack of symptoms or biomarkers enabling the early diagnosis of disease, and the propensity of malignant cells to seed the peritoneal cavity. (biomedcentral.com)
However, cell lines are known for their susceptibility to genetic and metabolic alterations, which can alter their characteristics in relation to the primary malignant cells. (biomedcentral.com)
Ideally, the more a cell line resembles the primary malignant cells, the more useful it will be for scientific research. (biomedcentral.com)

To prevent this from occurring, the new cell line may be immortalized using techniques such as the vinyl chloride immortalization method or transfection with genes such as SV40 T antigen or human telomerase. (atcc.org)
Since the mammalian cells do not uptake the foreign DNA efficiently, the availability of effective methods for introducing genes into the cells is essential. (benthamscience.com)
Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. (ubigene.us)
Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
Mutation signatures, genes with SNA or CNA of CSEC216 and published ESCC cell lines were similar with the mutation spectrum of original ESCC tumors. (biomedcentral.com)

Background information and genomic mutation data of published ESCC cell lines were obtained from online databases. (biomedcentral.com)
Genomic mutation features of CSEC216 and 28 ESCC cell lines were characterized which provided thorough cytogenetic background that facilitated future usage. (biomedcentral.com)
The current study also reported the genomic similarity of 28 ESCC cell lines and original ESCC tumors. (biomedcentral.com)

The ocular lens and its complement of epithelial cells are adapted to exist under hypoxic conditions that would otherwise injure most types of cell. (molvis.org)

We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
The SeqStudio instrument is easy to use across a broad range of applications, including de novo , targeted, and plasmid Sanger sequencing, CRISPR-Cas9 genome editing confirmation, cell line authentication, and detection of single nucleotide polymorphisms (SNPs). (thermofisher.com)

3T3 cells - a mouse fibroblast cell line derived from a spontaneous mutation in cultured mouse embryo tissue. (wikipedia.org)

We use standardized techniques which detect misidentified, genetically drifted, or cross-contaminated cells that invalidate research results. (huludirectory.com)
This allows an analysis to be repeated many times on genetically identical cells, which is desirable for repeatable scientific experiments. (wikipedia.org)
Further, cell lines can change genetically over multiple passages, leading to phenotypic differences among isolates and potentially different experimental results depending on when and with what strain isolate an experiment is conducted. (wikipedia.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)

With clear provenance information and authentication testing of early samples, it is possible to resolve debates regarding the origins of problematic cell lines that are widely used in cancer research. (providence.org)
Many cell lines that are widely used for biomedical research have been contaminated and overgrown by other, more aggressive cells. (wikipedia.org)
HeLa cells - a widely used human cell line isolated from cervical cancer patient Henrietta Lacks. (wikipedia.org)
Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. (biomedcentral.com)
Human cell lines are now widely used in laboratories worldwide. (biomedcentral.com)

Since we heard of the allegations that our stem cell lines came from Mizmedi, we turned to address that possibility. (blogspot.com)
Our team is thawing five frozen stem cell lines to authenticate our performance. (blogspot.com)
In addition, most of the stem cell lines were turned to stem cells of which the existence and DNA characteristics were not disclosed. (blogspot.com)

Subculturing, or passaging, is a critical part of banking and ensures the propagation of the cell line. (atcc.org)

For people researching in biological and medical fields, Cell Line Authentication is one of the essential tools that can help to study without any cell line errors. (huludirectory.com)
General strengths and weaknesses could include the rigor of the previous experimental designs, as well as the consideration of relevant biological variables and authentication of key resources. (nih.gov)
The last area of rigor and transparency involves authentication of key biological and/or chemical resources . (nih.gov)
the premier global biological materials resource and standards organization whose mission focuses on the acquisition, authentication, production, preservation, development, and distribution of standard reference microorganisms, cell lines, and other materials. (bibliotecapleyades.net)
Cell culture contaminations are a serious problem in biological research. (multiplexion.de)

The origins of some immortal cell lines, for example HeLa human cells, are from naturally occurring cancers. (wikipedia.org)
Major examples include human HeLa, the line is derived from cervical cancer cells taken on February 8, 1951 from Henrietta Lacks, a 31-year-old African-American mother of five, who died of cancer on October 4, 1951. (wikipedia.org)
The first continuous cell line Hela, was established in 1952 (7). (biomedcentral.com)
reported that many cell lines had been switched or cross-contaminated with Hela cells. (biomedcentral.com)

Cell culture is a fundamental tool used in areas as wide-ranging as drug development, toxicity testing, disease modeling, and regenerative medicine. (atcc.org)
Other important aspects of cell culture include 3D culture, engineering cell lines , and cell line authentication . (atcc.org)
Multiple methods of isolating cells for culture exist. (atcc.org)
A culture medium provides the cells with most of the nutrients such as amino acids, carbohydrates, vitamins, and minerals they need for metabolism. (atcc.org)
M14 samples were cryopreserved in December 1975, before MDA-MB-435 cells were established in culture. (providence.org)
the cells can be grown indefinitely in culture. (wikipedia.org)
The Cell Culture and Cell Phenotyping (CCCP) laboratories are located within the Department of Ophthalmology on the 7th floor of the Florence (E) Building. (utsouthwestern.edu)
This led to the basis of cell culture studies, where cells grown in controlled artificial conditions simulate the conditions prevailing in natural ones, therefore, presumed to act as those in in vivo conditions. (benthamscience.com)
In the following chapter, we will discuss the basics of cell culture including the equipments, chemicals and different types of materials required for cell culture. (benthamscience.com)
The in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. (biomedcentral.com)
Solicited models include patient-derived cell lines, patient-derived xenograft (PDX) mouse models, and 3D human tissue model culture systems established from racially/ethnically diverse patient populations. (sbir.gov)
3D human tissue model culture systems: While immortalized cell lines have been standard practice in cancer research for decades, adequate modeling of the heterogeneity of human cancer is an unmet need. (sbir.gov)
The Department of Human and Animal Cell Lines offers a broad range of cell culture related services. (dsmz.de)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)
Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of VEGF in cell culture medium. (molvis.org)
What is cell culture? (integra-biosciences.com)
Cell culture refers to the process of growing cells under controlled conditions outside their natural environment. (integra-biosciences.com)
1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)

The cells are generally grown in plastic or glass vessels. (atcc.org)
The cells can therefore be grown for prolonged periods in vitro. (wikipedia.org)
In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)

A variety of analytical approaches have indicated that melanoma cell line UCLA-SO-M14 (M14) and breast carcinoma cell line MDA-MB-435 originate from a common donor. (providence.org)
To address this question, we obtained early samples of the melanoma cell line (M14), a lymphoblastoid cell line from the same donor (ML14), and donor serum preserved at the originator's institution. (providence.org)
Through a series of molecular characterizations, including short tandem repeat (STR) profiling and cytogenetic analysis, we demonstrated that later samples of M14 and MDA-MB-435 correspond to samples of M14 frozen in 1975, to the lymphoblastoid cell line ML14, and to the melanoma donor's STR profile, sex and blood type. (providence.org)

The integrity of cell lines used in scientific research has been shown to be compromised in many studies (described in an open letter signed by leading scientists). (dnacenter.com)
Top scientific organizations are now issuing guidelines regarding the standards for validating the identity of cell lines used in publicly funded research and in studies submitted for publication to scientific journals. (dnacenter.com)
Solutions for manufacturing cell therapy products must be designed for safety, process flexibility, and scale-up from research through commercial manufacturing. (labmanager.com)
Download this resource guide to learn how a closed cell-processing system for cell therapy manufacturing can seamlessly scale with your process from research through commercial manufacturing. (labmanager.com)
The development and validation of appropriate cell and animal-based models to study underrepresented population groups would greatly advance this field of research. (sbir.gov)
Cancer cell lines: The use of immortalized cell lines in cancer research has been standard practice for decades. (sbir.gov)
Notably, the scientific integrity of cancer cell lines is critical for maintaining high standards in research. (sbir.gov)
At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
Information about authentication of research resources will be evaluated by the peer review panel as acceptable or not, but will not be factored into the final impact score. (nih.gov)

Molecular methods targeted to DNA mitochondrial (mtDNA) has been used mainly for identifying or confirming the species of origin of animal cell lines. (fapesp.br)
To establish penile cancer (PeCa) cell lines for the study of molecular mechanisms of carcinogenesis and testing therapeutic reagents. (oncotarget.com)
These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)

The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)

An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. (wikipedia.org)
CRCs have marked benefits over traditional immortalized cell lines as they are generated using special in vitro conditions that permit cells to pharmacologically bypass replicative senescence without any detectable cell crisis. (sbir.gov)

Because of its essential roles in cell signaling, misregulation or overexpression of EGFR often causes a cancerous phenotype. (biorxiv.org)

The Emerging Field of Medicines Authentication by Nuclear Quadrupole Resonance Spectroscopy (Counterfeit Medicines Volume II: Detection, Identification and Analysis, ILM Publications, 2013). (lu.se)

The document replaces a model policy for authentication of cell lines and tissue samples that was developed previously by the same authors for this purpose. (iclac.org)
The alternative, performing an analysis on primary cells from multiple tissue donors, does not have this advantage. (wikipedia.org)
While immortalised cell lines often originate from a well-known tissue type, they have undergone significant mutations to become immortal. (wikipedia.org)
The epidermal growth factor receptor (EGFR) is a HER-family receptor tyrosine kinase (RTK) that is involved in cell signaling in healthy tissue. (biorxiv.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)

Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
SB216763-treated HLE-B3 cells caused marked inhibition of GSK-3β activity prompting a significant increase in the translocation of cytoplasmic β-catenin to the nucleus. (molvis.org)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
We successfully established two PeCa cell lines from fresh tumor tissues from 21 cases. (oncotarget.com)
lncRNA XIST was highly expressed in hepatic carcinoma tissues and cells. (spandidos-publications.com)

This method ensures the protection of original stock and the consistent availability of low-passage vials of cells via the creation of a master cell bank and working cell banks. (atcc.org)

It is very important to confirm the identities of cell lines as part of quality control in laboratories that work and supply these cells lines. (fapesp.br)
Usually, cell lines have the facility to be cultivated, stored and shared between different laboratories. (biomedcentral.com)

The techniques used for maintenance, preservation and authentication of cell lines are also included. (benthamscience.com)

Misidentified and contaminated cell lines undermine your experimental results and discredit preclinical studies. (atcc.org)

The current method for cell line authentication is genotyping based on short tandem repeat (STR)-PCR involving coamplification of a panel of STR loci by multiplex PCR and downstream fragment length analysis (FLA), usually performed by capillary electrophoresis. (port.ac.uk)

There are several methods for generating immortalised cell lines: Isolation from a naturally occurring cancer. (wikipedia.org)
Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)

How many human cell line samples would you like to be analysed? (eurofinsgenomics.eu)

Hybridoma technology, specifically used for generating immortalised antibody-producing B cell lines, where an antibody-producing B cell is fused with a myeloma (B cell cancer) cell. (wikipedia.org)
A549 cells - derived from a cancer patient lung tumor. (wikipedia.org)
We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma. (biomedcentral.com)
Hepatoma cell lines (SUN449), hepatoblastoma cell line (HepG2, Huh‑6), liver cancer cell line (HepG2) and transformed human liver epithelial‑2 cells (THLE‑2) were used in the present study. (spandidos-publications.com)

We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)

A preliminarily study of carcinogenesis and chemosensitivity suggests that this cell model carries a tumor suppressor gene mutation and is sensitive to chemotherapy drugs. (oncotarget.com)
Biodistribution (BD) studies are critical components when assessing the preclinical safety evaluation of cell and gene therapy molecules. (generi-biotech.com)
The cell lines in our cell bank have been successfully used in gene-editing cell line generation, applicable for all kinds of gene-editing experiments. (ubigene.us)
At first glance, the commonality among synthetic gene networks, nerve cell response times and the emergence of antibiotic resistance is obscure. (biologists.com)

The cells are used for a wide variety of purposes, from testing toxicity of compounds or drugs to production of eukaryotic proteins. (wikipedia.org)
The purpose of this study was to identify potential therapeutic strategies to slow down or prevent the expression of early-onset epithelial to mesenchymal transition (EMT) marker proteins (fibronectin and alpha smooth muscle actin, α-SMA) without sacrificing the synthesis and accumulation of the prosurvival protein vascular endothelial growth factor (VEGF) in cultured virally transformed human lens epithelial (HLE) cells. (molvis.org)

Our data indicated that PET1 inhibits EGFR-mediated cell migration. (biorxiv.org)

Anatomy10

Organisms8

Diseases7

Chemicals and Drugs17

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Psychiatry and Psychology1

Phenomena and Processes30

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture1

Humanities1

Information Science14

Health Care7

Mycoplasma6

Contamination13

Characterization3

Proliferation13

Apoptosis4

Cultures14

Assays7

Biology8

Viability3

Researchers7

Tumor cell2

Squamous cell carc3

Misidentification3

Morphology2

Multi-factor authentication1

Somatic cell2

Vitro Cell Culture1

Laboratory10

Cryopreservation2

Malignant8

Genes5

Genomic3

Epithelial cells1

Plasmid2

Fibroblast1

Genetically4

Widely5

Stem Cell Lines3

Propagation1

Biological5

HeLa4

Culture26

Grown3

Melanoma3

Research9

Molecular3

Flow Cytometry1

Senescence2

Phenotype1

Detection1

Tissue5

Inhibition2

Tissues3

Vials1

Laboratories2

Preservation1

Experimental1

Genotyping1

Methods2

Human cell line1

Cancer6

Cellular1

Gene4

Proteins2

Migration1