Cell lines expressing. Medical search. Frequent questions

T cells stably expressing SARS-CoV Spike Glycoprotein. (topsan.org)
T6796CHO-K1 cells were stably infected with Human ACE2. (topsan.org)
T6029293T cells stably expressing Human ACE2Applications:For Research Use OnlyStorage Condition:Liquid nitrogen vapour phase. (topsan.org)
Researchers introduced an optimized plasmid to form the virus-like particles (VLPs) or non-structural protein 1 (NS1) into the cells and selected stably expressing cell populations for use to produce diagnostic antigens or immunogens for human and animal flavivirus vaccines. (nih.gov)
The chimeric IgM produced from the stably expressing HEK293 cell line was evaluated for use as a surrogate human-positive control in a serologic diagnostic test. (cdc.gov)

This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
Hence, we generated monoclonal antibodies by using UVC-irradiated apoptotic cells of an HCC cell line, HUH7, aiming to explore proteins differentially expressed in tumors and apoptosis. (deu.edu.tr)
Moreover, cellular proteins recognized by the antibody displayed a late upregulation in UVC- induced apoptotic cells. (deu.edu.tr)
Materials and Methods: Solid HNSCC tumors, as well as permanent HNSCC cell lines, were analyzed by flow cytometry concerning the expression of different putative stem cell marker proteins. (uni-luebeck.de)
Lifetime distribution for endocytic proteins was determined by the elapsed time between the appearance and disappearance of fluorescent structures present in the time series (6 min) from TIRF microscopy time-lapse videos of (a) BSC-1, (b) SK-MEL-2 CLTA-RFP and (c) SK-MEL-2 DNM2-GFP cell lines. (accessibletech4all.org)
Hi, does anybody has a good or bad experience with any of these plasmids expressing fusion proteins between EGFP and Neo or Hyg resistance. (bio.net)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

BC-related differentially expressed microRNAs (DE-miRs) were recognized and assessed in the present study. (nature.com)
However, we found that the proportions of Phox2a cells that expressed these genes were similar to the proportions reported for all lamina I ALS neurons, suggesting that Phox2a is not differentially expressed among cells belonging to these populations. (gla.ac.uk)

Multiple transporters are involved in this uptake as Chinese hamster ovary or HEK293 cells expressing either OATP1B1 ( K m = 1.4-3.5 μM), OATP2B1 ( K m = 0.7-0.8 μM), or OATP1B3 showed significant fluvastatin uptake relative to control cells. (aspetjournals.org)

MG-63, a cell that has fibroblast morphology isolated from the bone of a White, 14-year-old male patient with osteosarcoma. (atcc.org)
Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)
The time-lapse video shows live-cell imaging of an actin-GFP expressing mouse fibroblast responding to PDGF with circular dorsal ruffles. (cellimagelibrary.org)

Mechanical signaling plays many roles in cell physiology, including stem cell differentiation and reprogramming. (kerafast.com)
This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours. (torvergata.it)
These findings suggest that PDGF isoforms are involved not only in the promotion of the neuroblastoma cell growth, but also in neuronal cell migration, growth, and differentiation in human brain development. (jci.org)
Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
Cells were labeled with antibodies to the differentiation markers indicated. (jci.org)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

These specially tuned fields have no effect on quiescent cells while having an anti-proliferation and destructive effect on mitotic cells. (biomedcentral.com)
In vivo, poly(I:C) treatment increased intratumoral chemokine expression, NK-cell activation and tumor infiltration, and proliferation of tumor-infiltrating T and NK cells. (nih.gov)
Proliferation of tumor parenchyma cells was decreased. (nih.gov)
The inhibition of proliferation was associated with a decrease in the S-phase of the cell cycle and an accumulation of cells in the G2/M phase. (tau.ac.il)
This study demonstrates an additional cellular target of IM, not necessarily mediated via known tyrosine kinases, that causes inhibition of TA and cell proliferation. (tau.ac.il)
METTL13 and eEFlAK55me2 are Highly Indicated in Pancreatic and Lung Cancers and Promote Malignancy Cell Proliferation, Related to Number 4 (A) Summary of expression levels in six publicly available expression data units of PDAC (n=294 tumors and n=141 normal tissue independent samples). (epf2013.org)
MiaPaCa-2 and CFPAC-1 cells were treated with 100 ng/ml of NGF or the NGF inhibitor Tanezumab for 24 h, CCK-8 and Transwell assays were employed to test cell proliferation, invasion, and migration, respectively. (hindawi.com)
PANX1 channel inhibition using probenecid (PBN) or carbenoxolone (CBX) reduced the proliferation of our panel of high-risk NB cell lines. (jcancer.org)
We show that expression of the Y10F PANX1 mutant, which cannot be phosphorylated on tyrosine 10 and acts in a dominant-negative manner, curtailed NB cell proliferation. (jcancer.org)

Here we report the production of full-length PfRH5 protein using a cGMP-compliant platform called ExpreS 2 , based on a Drosophila melanogaster Schneider 2 (S2) stable cell line system. (nature.com)
Conclusion: Our data showed that L-cells efficiently produced the mature insulin protein. (upm.edu.my)
For the base, CDC researchers adapted human embryonic kidney 293 (HEK-293) cells, which are FDA-approved for use in producing biologics, in a serum-free and animal protein-free culture media, then propagated these as suspension cells. (nih.gov)
In the present study, to develop a new cancer cell line that is applicable to bioluminescence and fluorescence imaging, we transfected the expression vector of a green fluorescent protein ZsGreen1 into a metastatic cell line 5a-D-Luc, which is a subclone of the MDA-MB-231 breast cancer cell line expressing luciferase, and established a new tumor cell line 5a-D-Luc-ZsGreen expressing both luciferase and ZsGreen1. (elsevierpure.com)
We obtained 18 hybridoma clones recognizing protein targets in apoptotic HUH7 cells, and clone 6D5 was chosen for characterization studies because of its strong reactivity in cell-ELISA assay. (deu.edu.tr)
However, the distribution of the postsynaptic protein Homer revealed that dendritic spines accounted for a minority of the excitatory synapses on these cells. (gla.ac.uk)
FAM171B is a novel polyglutamine protein widely expressed in the mammalian brain. (nih.gov)
Physique S4 Endocytic protein lifetime distribution of genome-edited cell lines. (accessibletech4all.org)
I'm interested in using them in Jurkat cells after cloning the fusion protein into a retroviral vector, under the control of the retroviral promoter. (bio.net)
IDO is an immune suppressive protein expressed in glioma cells. (lu.se)

Individual analysis of 'tumor stem cell' markers will be an important tool for innovative therapies and for determining the prognosis of patients with HNSCC. (uni-luebeck.de)
Further, exosomes were extracted from PC cells and identified by examining the levels of specific markers for exosomes. (hindawi.com)
Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
Are both markers well expressed, in what cell lines, any toxicity. (bio.net)
CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)

Since epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors, including gefitinib (GEF) have been reported to induce the apoptosis of several cancer cell lines, in the present study, we examined whether the cytotoxic effects of GEF are further enhanced under amino acid starvation (AAS) culture conditions. (spandidos-publications.com)
Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)

The approval of KEYTRUDA as a first treatment instead of chemotherapy for patients who express high levels of PD-L1 has the potential to transform the way metastatic non-small cell lung cancer is treated," said Dr. Roy Baynes, senior vice president, head of clinical development, and chief medical officer, Merck Research Laboratories. (merck.com)

TLR3 expression in patient samples correlated with NK-cell activation, NK- and T-cell tumor infiltration, and inversely correlated with tumor parenchyma cell viability. (nih.gov)
In the present studies, we examined the expression of two PDGF receptor genes in human tumor cell lines derived from neural crest. (jci.org)
The expression of alpha and/or beta PDGF receptors was detected in a wide variety of neural crest-derived human tumor cell lines such as neuroblastoma, primitive neuroectodermal tumor, and Ewing's sarcoma by RNA blot analysis, and confirmed by immunoblot analysis. (jci.org)
Results: In this study, L-cells were isolated from a primary intestinal cell line to create suitable target cells for insulin expression studies. (upm.edu.my)
The secretion tests revealed that an increase in glucose concentration from 5 mM to 25 mM induced insulin gene expression in the L-cells by 2.7-fold. (upm.edu.my)
Some VLP-expressed clones to date have stayed in continuous culture and maintained VLP expression level for a year without sign of gene expression deterioration. (nih.gov)
Expression of wild type GR in COR L24 cells restored glucocorticoid signaling, with marked induction of GMCO reporter gene expression by dexamethasone (9,100±910%, n = 3), and an estimated EC50 of 10 nM. (manchester.ac.uk)
To identify human PPARs-responsive genes, we established tetracycline-regulated human hepatoblastoma cell lines that can be induced to express each human PPAR and investigated the gene expression profiles of these cells. (biomedcentral.com)
We found that the expression of each PPAR subtype was tightly controlled by the concentration of doxycycline in these established cell lines. (biomedcentral.com)
The real-time RT-PCR was used to verify the altered expression of selected genes by PPARs and we found that these genes were induced to express in the same pattern as detected in the microarray analyses. (biomedcentral.com)
In normal epidermis, β1 integrin expression is confined to the basal layer, whereas in hyperproliferative epidermis, integrins are also expressed in the suprabasal layers. (jci.org)
TrkA expression was knocked down in MiaPaCa-2 and dorsal root ganglion (DRG) cells treated with NGF to determine its effect on the Warburg effect. (hindawi.com)
Mechanistically, NGF could upregulate TDE-miR-21-5p levels, and DRG cells took up TDE to activate the Warburg effect and stimulate nociceptor gene expression. (hindawi.com)
These findings displayed that NGF/TrkA encouraged the neuroinvasive potential of PC cells by activating the Warburg effect in DRG cells through upregulation of TDE-miR-21-5p expression. (hindawi.com)
Previous studies have defined neurochemical populations among the ALS cells, based on expression of Tac1 or Gpr83. (gla.ac.uk)
Here, we show that while PANX1 is expressed in NB of all stages, high PANX1 expression in high-risk NB is associated with a reduced survival probability. (jcancer.org)
Flow cytometric analysis provided essential information of the cell line HLA-1 expression. (helsinki.fi)
Additional information of PD-L1 expression can be used to evaluate cell line's immune-evasion abilities. (helsinki.fi)
The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines. (lu.se)
Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)
This approach helped us to address these highly repetitive sequences, usually masked in standard bioinformatics pipelines, allowing us to accurately measure LINE-1 expression in each cell type found in our samples," explains Raquel Garza, co-first author of the study. (lu.se)

DOI=10.1073/pnas.81.2.568 Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.Proc. (mskcc.org)
DOI=10.1073/pnas.79.18.5641 Cairncross J.G., Mattes M.J., Beresford H.R., Albino A.P., Houghton A.N., Lloyd K.O., Old L.J. Cell surface antigens of human astrocytoma defined by mouse monoclonal antibodies: identification of astrocytoma subsets. (mskcc.org)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Results: Distinct populations of CD44 expressing potential stem cells could be identified in solid tumors of HNSCC patients with strong individual deviations. (uni-luebeck.de)

The results indicate that TTFields alone and in combination with paclitaxel and doxorubicin effectively reduce the viability of both wild type and MDR cell sub-lines and thus can potentially be used as an effective treatment of drug resistant tumors. (biomedcentral.com)
In addition to these four pathways, tumors can undergo infiltration around nerve fibers and metastasis along nerves, that is, perineural invasion (PNI), which refers to the phenomenon of perineural invasion by tumor cells filling the perineurial space, wrapping around nerves in a continuous concentric sheath-like pattern, infiltration, and metastasis of extension along nerves around nerve fibers or into perineurium within the perineurium [ 7 ]. (hindawi.com)
In August 2016, KEYTRUDA (pembrolizumab) (2 mg/kg every three weeks) was approved in Europe for previously-treated patients with locally advanced or metastatic NSCLC whose tumors express PD-L1 (TPS of 1 percent or more) and who have received at least one prior chemotherapy regimen. (merck.com)
The data demonstrate that KEYTRUDA provided meaningful improvements in survival versus the current standard of care in patients whose tumors express high levels of PD-L1," said Dr. Luis Paz-Ares, chair of the medical oncology department, Hospital Universitario Doce de Octubre, Madrid, Spain. (merck.com)
The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)

The addition of essential amino acids, but not non-essential amino acids to the cell culture medium resulted in the cancellation of this pronounced cytotoxicity. (spandidos-publications.com)

Surprisingly, the potential stem cell marker CD44 was found to be constitutively expressed on the surface of all the permanent HNSCC cell lines analyzed. (uni-luebeck.de)
This engineered human IgM constitutively expressed in a HEK-293 stable cell line can replace human-positive control sera in diagnostic serological techniques such as IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). (cdc.gov)

To study the migration of microglia across the BBB, we developed an in vitro co-culture system of mouse brain endothelial cells (MBECs) and Ra2 microglia using Transwell inserts. (portlandpress.com)
Human umbilical vein endothelial cells (HUVECS) labeled for focal adhesions (green) and F-actin (red). (cellimagelibrary.org)

DNA microarray analyses using these cell lines were performed with or without adding each subtype ligand and provided much important information on the PPAR target genes involved in lipid metabolism, transport, storage and other activities. (biomedcentral.com)
The results suggest that these cell lines are important tools used to identify the human PPARs-responsive genes. (biomedcentral.com)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
This Hydrogenophaga strain also expressed genes indicative of chemolithoautotrophy, including CO 2 fixation, H 2 oxidation, S-compound oxidation, and denitrification. (frontiersin.org)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Characterizing tumor cell lines. (helsinki.fi)

Both platelet-derived growth factor (PDGF) A- and B-chains are expressed in mammalian neurons, but their precise roles still remain to be clarified. (jci.org)
Reprogrammed cells could then develop into a multitude of cell types, including the neurons found in the brain and spinal cord. (livescience.com)
Cre mouse line has been shown to capture anterolateral system (ALS) projection neurons. (gla.ac.uk)
Here, we used this line to test whether Phox2a-positive cells represent a distinct subpopulation among lamina I ALS neurons. (gla.ac.uk)
This suggests that those ALS neurons that respond selectively to skin cooling, which have small cell bodies, may be included among the Phox2a population. (gla.ac.uk)
The neural crest is present during embryogenesis and gives rise to diverse cell types including enteric neurons and glia, as well as peripheral neurons [ 3 ]. (jcancer.org)
We observed that LINE-1s are highly expressed in the developing human brain and particularly in neurons in the adult human brain. (lu.se)

We have developed a glioma cell line with green fluorescent tumor cells that are inoculated into immunocompetent rats, and hereby we follow tumor cell infiltration. (lu.se)

Technology -CUTO-28 Cell Line (Colorado University Thoracic Oncology cell line, number 28), expressing an TPM3-ROS1 gene fusion. (technologypublisher.com)

NIH 3T3 cell expressing EGFP-Lifeact (a small genetically expressed probe that binds to actin and is derived from the first 17 aa of Abp140) imaged with structured illumination. (cellimagelibrary.org)
PKCa and PKCe activities were inhibited using isoform-specific pharmacologic inhibitors and genetically modified dominant-negative (DN) expressing cell lines. (cdc.gov)

The isolated cells displayed L-cell properties and were therefore used as an L-cell surrogate. (upm.edu.my)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)

COX-1 is expressed constitutively and is isolated throughout most cell lines in almost all mammalian tissues. (medscape.com)

PDGF isoforms as well as nerve growth factor induced morphological changes showing neuronal cell maturation. (jci.org)

Although the LAT family plays important roles in the development and function of normal tissues, they are frequently increased in cancer cells ( 3 ). (spandidos-publications.com)
Western blotting was employed to determine NGF level in PC and paracarcinoma tissues and in PC cell lines as well as pancreatic ductal epithelial cells. (hindawi.com)
NGF level was preeminently higher in PC tissues and cell lines than in paracarcinoma tissues and normal pancreatic epithelial cell lines. (hindawi.com)
Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
Pannexin 1 (PANX1) is expressed in many tissue types including tissues of neural origin. (jcancer.org)
Panx1 in rodents), which forms homo-heptameric single membrane channels permeable to ion and metabolite [ 7 , 8 ], is expressed in many tissue types including tissues of neural origin [ 9 ]. (jcancer.org)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

In addition, IM demonstrates activity against malignant cells expressing c-kit and platelet-derived growth factor receptor (PDGF-R). The activity of IM in the blastic crisis of CML and against various myeloma cell lines suggests that this drug may also target other cellular components. (tau.ac.il)
In the light of the important role of telomerase in malignant transformation, we evaluated the effect of IM on telomerase activity (TA) and regulation in various malignant cell lines. (tau.ac.il)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
Gross pathology reveals that the pleural surfaces are seeded with malignant mesothelioma cells, which form grouped nodules. (medscape.com)
Savic et al used fluorescence in situ hybridization (FISH) to distinguish malignant mesothelioma from reactive mesothelial cells in effusions. (medscape.com)

99% of all CD14 positive monocytes express CD163 and that monocyte derived dendritic cells express low levels of CD163. (nih.gov)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Johan Jakobsson, a professor at Lund University and research group leader at MultiPark and Lund Stem Cell Center, explains, "LINE-1 retrotransposons are a rich source of genetic sequences that we suspect have shaped the evolution of the human brain, and we now have the tools to explore their role in brain development. (lu.se)

Previous reports have indicated that CD163 is highly expressed on human macrophages, but found on less than 50% of peripheral blood monocytes. (nih.gov)
Many of the most highly expressed hydrolases belonged to a Ca . Bathyarchaeota strain and may have been associated with recycling of bacterial biomass. (frontiersin.org)

The numbers of viable cells in the treated cultures and the untreated control groups were determined using the XTT assay. (biomedcentral.com)
Anti-merozoite vaccine studies have long relied on the standardized in vitro assay of growth inhibition activity (GIA) 12 , whereby purified IgG antibodies are tested against parasites cultured in human red blood cells (RBC) in the absence of other cell types. (nature.com)
To determine the impacts of miR-183 on cell viability, the MTT assay was used. (nature.com)
To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
RIP assay was performed to validate NGF and miR-21 binding ability in MiaPaCa-2 cells. (hindawi.com)
Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)

Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
Cadaver-collected fibroblasts can be reprogrammed into induced pluripotent stem cells using chemicals known as growth factors that are linked with stem cell activity. (livescience.com)
Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
Successfully reprogramming induced pluripotent stem cells so they behave like the cells they are meant to replace means that samples of the mimicked cells must be present for comparison. (livescience.com)

These cell lines express actin force probes, allowing for the measurement of forces of actin and actinin in living cells via simple anisotropy FRET ratio R to measure changes in FRET. (kerafast.com)
Once expressed in cells, the actin probes incorporate into mainly into f-actin filaments and allow for the measurement of forces of actin and actin in in living cells via simple anisotropy FRET ratio R to measure changes in FRET. (kerafast.com)
Lysates of (a) BSC-1 or (b to d) SK-MEL-2 cells were immunoblotted against CLTA, DNM2, RFP, GFP, or actin. (accessibletech4all.org)
NG108-15 cells expressing EGFP-actin. (cellimagelibrary.org)

HCC cell death, and natural killer (NK) cell activation and cytotoxicity were assessed in vitro after treatment with the TLR3 ligand poly(I:C). The effect of TLR3 on the tumor parenchyma and infiltrating immune cells was investigated in a spontaneous liver tumor mouse model and a transplanted tumor mouse model (n = 3-9 mice per group). (nih.gov)
We have previously described an in vitro model using human SCLC cell lines that express POMC and are resistant to glucocorticoids. (manchester.ac.uk)
To reveal that the NGF-TrkA signaling pathway was closely associated with PC PNI, in vitro neuroinvasion model was established by using MiaPaCa-2 cells via coculturing DRG cells in Matrigel. (hindawi.com)
Dust samples collected from Nebraska swine confinement facilities (hog dust extract [HDE]) are known to elicit proinflammatory cytokine release from human bronchial epithelial (HBE) cells in vitro. (cdc.gov)

As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)
Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)

We have also demonstrated that PDGF receptors on the human neuroblastoma cell lines were biologically functional. (jci.org)
The neuroblastoma cell lines also expressed the transcripts of PDGF A- and B-chains. (jci.org)

The 5a-D-Luc-ZsGreen cells proliferate more rapidly and have a more invasive phenotype compared with 5a-D-Luc cells following intracardiac injection. (elsevierpure.com)
Surprisingly, scalp cells did proliferate more and grew more rapidly than dura mater cells. (livescience.com)

The glucagon-like peptide-1 (GLP-1) promoter, which is recognised by gut L-cells, is an appealing candidate for gene therapy purposes. (upm.edu.my)
Next, the isolated L-cells were transfected with the recombinant plasmid consisting of an insulin gene located downstream of the GLP-1 promoter. (upm.edu.my)
In conclusion, GLP-1 promoter and L-cell could be potential candidates for diabetes gene therapy agents. (upm.edu.my)
To analyze GR function both positive (GMCO) and negative (TRE)3-tkCAT), glucocorticoid-regulated reporter gene constructs were transfected into COR L24 cells. (manchester.ac.uk)
This failure of the GR explains the resistance of the POMC gene to glucocorticoid inhibition and may have implications for cell growth in SCLC. (manchester.ac.uk)

Top panel: The number of genotyped clones is usually given for each target in each cell type, along with the number (percentage) of correctly tagged clones having at least one (but less than all) allele tagged, or all alleles tagged. (accessibletech4all.org)
ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)

Autophagic cell death was excluded as the inhibition of autophagy did not attenuate the cytotoxicity. (spandidos-publications.com)
The purpose of this study is to investigate cell growth inhibition of wild type and the corresponding MDR cells by Tumor Treating Fields - TTFields, a new cancer treatment modality that is free of systemic toxicity. (biomedcentral.com)

Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)

Low-risk types (e.g., types 6 and 11) can cause benign or low-grade cervical cell changes, genital warts, and recurrent respiratory papillomatosis ( 4 ). (cdc.gov)
High-risk types (e.g., types 16 and 18) can cause low-grade cervical cell abnormalities, high-grade cervical cell abnormalities that are precursors to cancer, and cancers ( 5 - 7 ). (cdc.gov)
Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
We show that virtually all lamina I Phox2a cells can be retrogradely labelled from injections targeted on the lateral parabrachial area (LPb), and that most of those in the cervical cord also belong to the spinothalamic tract. (gla.ac.uk)

Through simply changing cell culture conditions, a newly established cell line, Erythroid Line from Lund University (ELLU), is able to differentiate toward mature cells, including enucleated reticulocytes. (lu.se)

Human neuroblastoma cells express alpha and beta platelet-derived growth factor receptors coupling with neurotrophic and chemotactic signaling. (jci.org)

hDNM2X, stable overexpression DNM2-EGFP cell collection. (accessibletech4all.org)
rCLTAX, stable GFP-CLTA (rat brain-derived) overexpression cell collection. (accessibletech4all.org)
It can also cause TAC in other conditions in which increased red cell production is necessary to maintain stable red cell indices, as may occur in anemia due to blood loss. (cdc.gov)

The identity of these cells and the underlying cellular and molecular mechanisms are mostly unknown in HNSCC to date. (uni-luebeck.de)

Western analysis with the indicated antibodies of WCEs from U2OS cells expressing the control sgRNA from your 322 sgRNA KMT library and seven randomly selected sgRNAs focusing on the potential KMTs in the human being genome. (epf2013.org)
We investigate this in other human cell lines and rat glioma cell lines, and run animal experiments where the complement inactivation is targeted with antibodies. (lu.se)

Exposure of cancer cells to chemotherapeutic agents may result in reduced sensitivity to structurally unrelated agents, a phenomenon known as multidrug resistance, MDR. (biomedcentral.com)
human breast cancer cells MCF-7 and their mitoxantrone-resistant sub lines MCF-7/Mx and human breast cancer cells MDA-MB-231 and their doxorubicin resistant MDA-MB-231/Dox cells. (biomedcentral.com)
Multidrug resistance (MDR) [ 1 ] is encountered when cancer cells are exposed to chemotherapeutic agents for a few replication cycles. (biomedcentral.com)
Three decades of multidrug resistance research have identified a number of mechanisms by means of which cancer cells elude the effects of chemotherapeutic agents. (biomedcentral.com)
The citral in lemongrass is also thought to have potent anticancer abilities against some cancer cell lines, research suggests. (express.co.uk)
This occurs either by causing cell death directly or boosting your immune system so that your body is better able to fight-off cancer on its own. (express.co.uk)
Understanding cancer cell dynamics in the metastatic process is important to develop new therapeutic strategies. (elsevierpure.com)
Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. (nature.com)
PTEN insufficiency promotes the PI3K/AKT path, which is a vital signal for the survival of cancer cells. (nature.com)
SK-MG-09 is a human brain cancer cell line. (mskcc.org)
Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
MiR-483-3p regulates oxaliplatin resistance by targeting FAM171B in human colorectal cancer cells. (nih.gov)
Another challenge is the cell entry of oncolytic adenovirus, which is mainly mediated by the Coxsackie-Adenovirus receptor and this receptor is downregulated in various cancer cells. (helsinki.fi)
In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)

By facilitating the engraftment of human tumor and immune cells, HIS models provide a platform for studying tumor-associated immunology, immunotherapy drugs, and tumor-immune cell interactions. (transcurebioservices.com)

NG108-15 cells are a cell line that is derived from a mouse neuroblastoma and a rat glioma. (cellimagelibrary.org)
Our co-operation partner Kurt Osther has seen that human glioma cells express factors suppressing the complement activation. (lu.se)

AZD5423 The putative hydrogen bonds are demonstrated as reddish dashed lines. (epf2013.org)

MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)

1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
Although most Phox2a cells phosphorylated extracellular signal-regulated kinases following noxious heat stimulation, ~ 20% did not, and these were significantly smaller than the activated cells. (gla.ac.uk)

The researchers found fibroblasts taken from the brain lining, or dura mater, were 16 times more likely to grow successfully than those from the scalp. (livescience.com)

PPARα is expressed in the liver, kidney, heart and muscle where it regulates energy homeostasis. (biomedcentral.com)

It is becoming increasingly obvious that stem cells play an important role in tumor development and progression. (uni-luebeck.de)
Conclusion: CD44+ 'tumor stem cells' may play a key role in the establishment of permanent HNSCC cell lines, selecting especially robust cell entities that might drive the progression and metastasis of HNSCC. (uni-luebeck.de)
Now scientists have harvested such cells from the scalps and brain linings of human corpses and reprogrammed them into stem cells. (livescience.com)

But Anglican Archbishop of Sydney Glenn Davies said there were ethical questions about the potential vaccine, because scientists have used cell lines from an electively aborted foetus. (abc.net.au)

98th percentile) transcripts, including acetone carboxylase and cell-wall-associated hydrolases with unknown substrates (numerous lesser expressed cell-wall-associated hydrolases targeted peptidoglycan). (frontiersin.org)

One major problem is that the tumor cells evade the patients' own immune response. (lu.se)

Anatomy34

Organisms12

Diseases36

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes64

Disciplines and Occupations1

Information Science3

Stably5

Proteins7

Differentially expressed2

HEK2931

Fibroblast5

Differentiation11

Proliferation9

Protein10

Markers5

Apoptosis2

Metastatic non-small1

Expression24

Antigens4

Populations1

Tumors5

Pronounced cytotoxicity1

Constitutively expressed2

Endothelial Cells2

Genes5

Tumor cell lines1

Neurons7

Infiltration1

Oncology1

Genetically2

Surrogate2

Mammalian1

Isoforms1

Tissues7

Malignant5

Dendritic Cells2

Lund Stem Cell Center1

Highly expressed2

Assay6

Pluripotent stem5

Actin4

Vitro4

Therapies4

PDGF2

Proliferate2

Gene5

Clones2

Inhibition2

Induce1

Cervical4

Differentiate1

Growth1

Stable3

Cellular1

Antibodies2

Cancer14

Immunology1

Glioma2

Putative1

Viability2

Replication1

Extracellular2

Fibroblasts1

Kidney1

Stem Cells3

Vaccine1

Transcripts1

Immune1