• Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
  • HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
  • High expression of RAD18 facilitated a highly stem-cell phenotype through the Hippo/YAP pathway, which supports the proliferation of TNBC. (nature.com)
  • pointed out that Mage-D1 may participate in the proliferation of bone marrow mesenchymal stem cells (BMSCs) and the differentiation of odontoblasts through the NF-κB signalling pathway 21 . (researchsquare.com)
  • K405R mutant expressing cancer cells have reduced G1/S transition and slower proliferation compared to wildtype. (oncotarget.com)
  • Collectively, these findings suggest that SAMHD1 acetylation enhances its dNTPase activity and promotes cancer cell proliferation. (oncotarget.com)
  • Lately, chimeric antigen receptor T cells (CAR-T cells) have been confronted with the issues of weak proliferation and poor persistence within the remedy of some malignancies. (cellgenetherapy.com)
  • Along with the participation of T cell receptor (TCR) and costimulatory alerts, immune cytokines additionally exert a decisive function within the activation and proliferation of T cells. (cellgenetherapy.com)
  • When CAR-T cells are in touch with goal tumor tissue, the proliferation means and persistence of T cells might be improved by structurally or inductively releasing immunoregulatory molecules to the tumor area. (cellgenetherapy.com)
  • EBVaGC is defined by monoclonal proliferation of carcinoma cells with latent EBV infection, as demonstrated by EBV-encoded small RNA (EBER) in situ hybridization. (spandidos-publications.com)
  • These abnormalities result in significant alterations in gene expression related to cell proliferation, apoptosis, migration and immune signaling pathways. (spandidos-publications.com)
  • Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
  • Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
  • Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
  • Our results show a novel role for DDX5 in cancer cell proliferation and suggest DDX5 as a therapeutic target in breast cancer treatment. (aacrjournals.org)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • Defects in the control of cell proliferation are a hallmark of cancer, and DNA replication is a key process for cell proliferation. (aacrjournals.org)
  • 2.2 EBV-Driven B-Cell Proliferation EBV illness of B cells results in the outgrowth of immortalized Clemastine fumarate lymphoblastoid B-cell lines (LCLs) which communicate the latency type 3 system. (colinsbraincancer.com)
  • These mimic external growth signals (LMP1 and LMP2) Clemastine fumarate or directly regulate gene manifestation (EBNA2 EBNA3c) therefore driving the infected cell into proliferation [7]. (colinsbraincancer.com)
  • In latency type 0 no EBV protein is definitely indicated in the infected cell [8 9 Induction of lytic replication in some of the latently infected cells leads to the production and launch of infectious viral progeny that can infect neighboring B cells therefore promoting virus distributing and EBV-associated B-cell proliferation [8]. (colinsbraincancer.com)
  • 2.3 Impaired Clemastine fumarate T-Cell Control of EBV-Induced B-Cell Proliferation EBV-infected B cells. (colinsbraincancer.com)
  • 2000 In T-cells calcium mineral Ebastine signaling is necessary for activation proliferation and differentiation mainly through the experience of NFAT (Nuclear Element of Activated T-cells) a transcription element that becomes triggered because of improved intracellular calcium mineral ([Ca2+]we) (Macian 2005 It's been demonstrated that NFAT activation is essential for the manifestation of genes necessary for proper T-cell activation (evaluated in Hogan et al. (ecologicalsgardens.com)
  • 2003 While TCR signaling regulates the activation of peripheral T-cells the next clonal proliferation necessary for a proper immune system response can be managed by Interleukin-2 (IL-2). (ecologicalsgardens.com)
  • Furthermore, the TNFRSF13B was knocked down in 22Rv1 and PC-3 human prostate cancer cell lines via transfecting short hairpin RNAs and cell proliferation and colony formation assays were performed. (biomedcentral.com)
  • ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
  • cells of two ebna (epstein-barr virus nuclear antigen)-negative human lymphoma cell lines, bjab and ramos, were infected with two strains of epstein-barr virus (ebv). (liverpool.ac.uk)
  • Epstein-Barr Virus Nuclear Protein 3C Domains Necessary for Lymphoblastoid Cell Growth: Interaction with RBP-J Regulates TCL1. (nordicmubio.com)
  • Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
  • In this study we focused our attention on the behavior of four nuclear matrix proteins during the various stages of apoptosis in the HL-60 cell line exposed to the DNA topoisomerase I inhibitor, camptothecin. (nih.gov)
  • Certain non-structural proteins and the major basic internal protein are the main phosphoproteins detected in infected cells. (microbiologyresearch.org)
  • Many cell proteins continue to be phosphorylated throughout infection. (microbiologyresearch.org)
  • Autographa californica nuclear polyhedrosis virus phosphoproteins and synthesis of intracellular proteins after virus infection. (microbiologyresearch.org)
  • MHC-I and MHC-II proteins are key components of the antigen presentation machinery responsible for neoantigen presentation to CD8 + and CD4 + T lymphocytes, respectively. (portlandpress.com)
  • By using model DNA templates, purified DNA pols beta and lambda and knockout cell extracts, we show here that the auxiliary proteins replication protein A and proliferating cell nuclear antigen act as molecular switches to activate the DNA pol lambda- dependent highly efficient and faithful repair of A:8-oxo-G mismatches in human cells and to repress DNA pol beta activity. (neb.com)
  • Eukaryotic expression vectors containing genes encoding plant proteins for killing of cancer cells. (weeksmd.com)
  • consequently, genes encoding some of these proteins are being used to design constructs for the inhibition of multiplying cancer cells. (weeksmd.com)
  • In addition, she used a genomewide CRISPR screen and identified HIV-1 silencing factors including SAFB family proteins and RNA nuclear exosome complex ( J Virol 2022 ). (yale.edu)
  • Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)
  • DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
  • This "growth program" is definitely characterized by the manifestation of nine proteins: three latent membrane proteins (LMPs) and six EBV-associated nuclear antigens (EBNAs). (colinsbraincancer.com)
  • These studies provided proof-of-concept data suggesting that adult stem cell-derived connective tissue proteins can participate directly in restoration of defective extracellular matrix and demonstrating the feasibility of systemic cellular therapy for DEB. (biomedcentral.com)
  • These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
  • The E3 ubiquitin ligase RAD18 is well known for the maintenance of genome stability and cell survival through many DNA damage response (DDR) pathways such as translesion DNA synthesis (TLS) and homologous recombination repair (HRR) [ 4 , 5 ]. (nature.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Their expression in tumour cells is modulated by a complex interplay of genomic, transcriptomic and post translational factors involving multiple intracellular antigen processing pathways. (portlandpress.com)
  • Demonstration of antigen to the TCR causes multiple signaling pathways required for T-cell activation (examined in Lin and Weiss 2001 Of particular importance is the activation of phospholipase C (PLCγ1) which hydrolyzes phosphatidyl 4 5 (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3). (ecologicalsgardens.com)
  • The primary action of DAG is definitely to activate Protein Kinase C (PKC) which can then activate downstream signaling pathways ultimately leading to the nuclear translocation of important transcription factors AP-1 and NF-κB (examined in Isakov and Altman 2002 In the mean time IP3 engages the IP3 receptor (IP3R) liberating calcium from the endoplasmic reticulum (ER). (ecologicalsgardens.com)
  • Furthermore, silencing TNFRSF13B expression resulted in decreased colony formation of 22Rv1 and PC-3 cells through modulating the cell cycle and p53 signalling pathways. (biomedcentral.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
  • Research has intensified to understand ERV protein function and their role as tumor antigens and targets for cancer (immune) therapy. (frontiersin.org)
  • SAM domain and HD domain containing protein 1 (SAMHD1) is a deoxynucleotide triphosphohydrolase (dNTPase) that inhibits retroviruses by depleting intracellular deoxynucleotide triphosphates (dNTPs) in non-cycling myeloid cells. (oncotarget.com)
  • Protein synthesis in cells infected by Autographa californica nuclear polyhedrosis virus (Ac-NPV): the effect of cytosine arabinoside. (microbiologyresearch.org)
  • Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. (microbiologyresearch.org)
  • Properties of the major nucleocapsid protein of Heliothis zea singly enveloped nuclear polyhedrosis virus. (microbiologyresearch.org)
  • Protein kinase activity associated with the extracellular and occluded forms of the baculovirus Autographa californica nuclear polyhedrosis virus. (microbiologyresearch.org)
  • Reference: Replication protein A and proliferating cell nuclear antigen coordinate DNA polymerase selection in 8-oxo-guanine repair. (neb.com)
  • EB06619 (1µg/ml) staining of HeLa (A) and Jurkat (B) cell lysate (35µg protein in RIPA buffer). (everestbiotech.com)
  • Strong expression of the protein seen in the cytoplasm and microtubules of HeLa cells. (everestbiotech.com)
  • EBNA 3C is a latent viral nuclear protein expressed in EBV transformed lymphoblastic cell lines and is essential for EBV mediated transformation of these cell lines. (nordicmubio.com)
  • This viral nuclear protein is essential for EBV mediated positive immunoblastic lymphomas, and functions as a transcription factor. (nordicmubio.com)
  • PM 2.5 increased the mRNA and protein expression of M1 markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. (medsci.org)
  • Overexpression of miR-146a-3p downregulated the expression of SIRT1 protein in untreated RAW264.7 cells. (medsci.org)
  • Importantly, inhibition of miR-146a-3p upregulated SIRT1 protein and suppressed M1 polarization in PM 2.5 treated RAW264.7 cells. (medsci.org)
  • Once these stores are depleted the ER-bound calcium sensor Stim1 (Stromal interaction molecule 1) couples the ER to the cytosolic Ca2+ channel protein Orai1 allowing extracellular calcium to enter the cell via store-operated calcium mineral admittance (SOCE) (evaluated in Feske 2007 Hogan et al. (ecologicalsgardens.com)
  • However, despite encouraging results, these trials revealed that the majority of curative effects resulted from the secretion of various paracrine factors elicited by transplanted cells rather than from donation of the therapeutic protein to the affected cutaneous tissue [ 11 ]. (biomedcentral.com)
  • PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. (ox.ac.uk)
  • Electrophoretic mobility shift assays (EMSA) identified oligonucleotide-protein binding for risk and non-risk SNPs to nuclear extracts of A549, BEAS 2B, and IMR-90 lung cell lines. (cdc.gov)
  • In animals receiving excitotoxic lesions of the striatum we detected an eightfold increase of green ¯uorescent protein (GFP)-expressing cells. (lu.se)
  • M, C4, C5 and C4-2 also expressed reduced human androgen receptor mRNA as expected in AI cells. (wikipedia.org)
  • In our previous studies, EMSCs were obtained from rat embryonic facial process tissue by fluorescence p75 neurotrophin receptor (p75NTR) activated cell sorting, providing a good stem cell model for studies of dental mineralization. (researchsquare.com)
  • Antigen engagement from the T-cell receptor (TCR) induces a rapid and dramatic decondensation of chromatin that is necessary for T-cell activation. (ecologicalsgardens.com)
  • These long-lived na?ve T-cells circulate in the periphery and remain quiescent until activated by presentation of a T-cell receptor (TCR)-specific antigen. (ecologicalsgardens.com)
  • These four pomegranate compounds inhibited DHT-stimulated androgen receptor nuclear accumulation and the expression of the androgen receptor-dependent genes prostate specific antigen and steroid 5α-reductase type 1 at concentrations ≥10 μM. (nih.gov)
  • Indirect immunofluorescence experiments were performed to follow these nuclear matrix antigens during apoptosis. (nih.gov)
  • While the antibody to the nucleolar isoform of DNA topoisomerase II gave a fluorescent pattern that was well-maintained until the late phases of apoptosis, the other three nuclear antigens showed marked modifications in their distribution. (nih.gov)
  • Indeed apoptosis is inhibited in senescent cells. (globale-dermatologie.com)
  • Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)
  • We determined the possible contribution of apoptosis to the observed decrease in cell growth and found that three compounds, EGCG, kaempferol, and, in particular, punicic acid, induced DNA fragmentation after a 24 h treatment, at concentrations in the 10-100 μM range. (nih.gov)
  • The malignant properties of LNCaP cells are maintained in athymic nude mice which develop tumors at the injection site and show a similar doubling time in vivo. (wikipedia.org)
  • In response to endogenous and exogenous insults, malignant cells mostly have an intensive DNA repair capacity that allows them to proliferate and survive. (nature.com)
  • By studying numerous literatures, we summarized the purposeful traits of some members of the interleukin household associated to tumor immunotherapy, and described the analysis standing of gene-edited interleukin CAR-T cells within the remedy of malignant tumors. (cellgenetherapy.com)
  • Computerized semi-automatic nuclear morphometry is an objective and reproducible tool for quantitative evaluation of nuclear features which may help to differentiate between benign and malignant thyroid lesions in cytology. (bvsalud.org)
  • Objective of the study was to apply morphometry with regard to nuclear size parameters, shape parameters and nuclear chromasia with derivation of suitable cutoff values to differentiate benign and malignant lesions of thyroid gland. (bvsalud.org)
  • In this study, all the nuclear size parameters and one nuclear shape parameter was found significant in differentiating benign and malignant lesions. (bvsalud.org)
  • Numerous vectors have been engineered for the sole purpose of killing cancer cells, and some have successfully suppressed malignant tumours. (weeksmd.com)
  • Therefore, the authors concluded to a probable hyperplasic status in benign or malignant neoplasic cells. (globale-dermatologie.com)
  • Immunodeficiencies are genetic diseases known to predispose an individual to cancer owing to defective immunity towards malignant cells. (biomedcentral.com)
  • Collectively our data suggest that chromatin decondensation in peripheral T-cells is definitely controlled by modulating intracellular calcium levels. (ecologicalsgardens.com)
  • EBV was the first virus to be associated with human malignancy, which was discovered from a Burkitt's lymphoma cell line in 1964 ( 1 ). (spandidos-publications.com)
  • Subsequent studies revealed that EBV caused a number of different human malignancies, such as nasopharyngeal carcinoma (NPC), Hodgkin's lymphoma, extranodal NK/T-cell lymphoma, nasal type and lymphoproliferative disorders of immunocompromised hosts ( 2 ). (spandidos-publications.com)
  • Peripheral blood mononuclear cells of healthy donors and post-transplant lymphoma patients were stimulated with or without lunasin in the presence of IL-12 or IL-2. (weeksmd.com)
  • The combination of lunasin and cytokines (IL-12 plus IL-2) was capable of restoring IFNγ production by NK cells from post-transplant lymphoma patients. (weeksmd.com)
  • establishment of ebna-expressing cell lines by infection of epstein-barr virus (ebv)-genome-negative human lymphoma cells with different ebv strains. (liverpool.ac.uk)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody (1D2) [NBP1-54446] - SREBP2 (1D2) antibody was tested in HeLa cells with DyLight 488 (green). (novusbio.com)
  • EB06619 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. (everestbiotech.com)
  • The Langerhans' cells (LC) were identified with a monoclonal antibody to OKT6 antigen, by employing an immunofluorescence technique. (medicaljournals.se)
  • Parental LNCaP, M, C4, and C5 subline have similar baseline gene expression levels of ornithine decarboxylase (ODC) and Prostate Specific Antigen (PSA) however, M, C4, and C5 sublines express 5-10X more PSA mRNA. (wikipedia.org)
  • Mage-D1, also known as Dlxin-1 or NRAGE, was first cloned and identified as a new member of the type II melanoma-associated antigen gene family by Pold et al. (researchsquare.com)
  • There are numerous CAR-T cells research on gene-edited cytokines, and the most typical cytokines concerned are interleukins (IL-7, IL-12, IL-15, IL-18, IL-21, IL-23). (cellgenetherapy.com)
  • Strategies for the development of gene-edited interleukin CAR-T cells embrace co-expression of single interleukin, two interleukin, interleukin mixed with different cytokines, interleukin receptors, interleukin subunits, and fusion inverted cytokine receptors (ICR). (cellgenetherapy.com)
  • The target is to discover the optimized technique of gene edited interleukin-CAR-T cell perform. (cellgenetherapy.com)
  • Quite a lot of cell transplantation and gene remedy trials have been carried out during the last three many years in an effort to revive perform in Parkinson's illness. (cellgenetherapy.com)
  • The effects on dopamine (DA) release and dopamine transporter (DAT) gene expression were assayed in hypothalamic HypoE22 cells. (researchgate.net)
  • In the same experimental system, IL-10 and BDNF gene expressions increased, and in HypoE22 cells, the extract decreased the extracellular dopamine level and increased the DAT gene expression due to the direct interaction of parthenolide with the DAT. (researchgate.net)
  • The underlying mechanism responsible for the effects of lunasin on NK cells is likely due to epigenetic modulation on target gene loci. (weeksmd.com)
  • Gene therapy has attracted attention for its potential to specifically and efficiently target cancer cells with minimal toxicity to normal cells. (weeksmd.com)
  • Indeed, in a clone of cells, only one of the alleles is expressed, because the same X chromosome is inactivated by lyonisation{Allen, 1992}, in a clone, whereas in normal {Nakamura, 2001} skin (polyclonal), the inactivated HUMARA gene allele isn't always on the same X chromosome. (globale-dermatologie.com)
  • Directly after B-cell illness EBV establishes a nonproductive ("latent") infection that is divided into four types (latency type 0 to 3) characterized by unique viral gene manifestation profiles [6]. (colinsbraincancer.com)
  • The vast majority of these cells did not divide, suggesting that the transgene was indeed regulated in a similar fashion as the endogenous GFAP gene. (lu.se)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
  • Odontogenic keratocysts (OKCs) associated with NBCCS have occasionally been reported to transform into aggressive neoplasms such as ameloblastomas and squamous cell carcinoma . (medscape.com)
  • Ongoing research investigates mechanisms invoked by cancer cells to abrogate MHC-I expression and attenuate anti-tumour CD8 + cytotoxic T cell response. (portlandpress.com)
  • One such peptide, Lunasin , has been shown to selectively act on newly transformed cells while having no cytotoxic effect on non-tumorigenic or established cancer cell lines. (weeksmd.com)
  • As a postdoc, she profiled HIV-1 DNA and RNA landscape and identified the impact of cytotoxic T lymphocytes (CTLs) and defective HIV-1 proviruses on HIV-1 persistence ( Cell Host Microbe 2017 , Best Paper of the Year, corresponding author). (yale.edu)
  • She found that HIV-1 preferentially persist in cytotoxic CD4 + T cells. (yale.edu)
  • The Nuclear morphometry may be used as an adjunctive tool to routine thyroid cytology. (bvsalud.org)
  • Metastasis of clear cell renal cell carcinoma (ccRCC) to the thyroid gland is rare, and simultaneous occurrence of ccRCC and papillary thyroid carcinoma (PTC) is even rarer. (spandidos-publications.com)
  • By using an immortalized human fibroblast cell line that has the potential to induce cancer in mice, we show that the development of a tumoral phenotype in these cells correlated with a differential expression of DNA pols lambda and beta. (neb.com)
  • Antibodies against HHV-8 latency-associated nuclear antigen were identified by immunofluorescent antibody assay by using the BC3 cell line ( 3 ). (cdc.gov)
  • sera from children were tested for antibodies to viral capsid antigens of epstein-barr virus. (liverpool.ac.uk)
  • Western Blot: SREBP2 Antibody (1D2) [NBP1-54446] - Lane1: HEp-2 cells. (novusbio.com)
  • Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (ecceterra.fr)
  • IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. (everestbiotech.com)
  • The page also collects GeneMedi's different modalities and formats products for PGR in therapeutics/drug discovery and IVD diagnostics, which is including antibody, ADC, bispecific, antigen, ORF vector, VLP, etc. (genemedi.net)
  • Pre-made anti-PGR monoclonal antibody(mab) is expressed by mammalian cell line as a benchmark antibody for cell culture, ELISA or other affinity binding assay or functional assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic). (genemedi.net)
  • The anti-PGR mab is expressed and produced by mammalian cell line as a benchmark reference therapeutic antibody for biological drug discovery items including cell culture, assay development, animal model development, PK/PD model development (Pharmacokinetics & Pharmacodynamic) and mechanism of action (MOA) research. (genemedi.net)
  • The monoclonal antibody Ki-67 was used to determine the numbers of cycling cells in hair follicles both in alopecia areata and in normal scalp skin. (medicaljournals.se)
  • The LNCaP (Lymph Node Carcinoma of the Prostate) cell line was established from a metastatic lesion of human prostatic adenocarcinoma. (wikipedia.org)
  • Renal cell carcinoma (RCC) is one of the ten most common cancers for men and women with an approximate 75% overall 5-year survival. (frontiersin.org)
  • Sixteen histological tumor subtypes exist and the most common are papillary, chromophobe and clear cell renal cell carcinoma (ccRCC) representing 85% of all RCC. (frontiersin.org)
  • The odontogenic keratocyst (OKC) is known for its high recurrence rate, aggressive behavior, and its occasional association with the nevoid basal cell carcinoma syndrome (NBCCS). (medscape.com)
  • Panoramic film demonstrating mandibular odontogenic keratocysts in nevoid basal cell carcinoma syndrome. (medscape.com)
  • Basal cell carcinoma is the most frequently occurring cancer in humans. (bvsalud.org)
  • This review focused on contemporary evidence on basal cell carcinoma cytology to provide a comprehensive description of the technique with practical insights for specific clinical scenarios. (bvsalud.org)
  • Mucinous tubular and spindle cell carcinoma of the kidney is a rare and a recently described tumor with a favorable prognosis. (journalmc.org)
  • Four cases of mucinous tubular and spindle cell carcinoma of the kidney were diagnosed in our Department of Pathology (Farhat Hached hospital, Sousse, Tunisia). (journalmc.org)
  • The histological diagnosis of mucinous tubular and spindle cell carcinoma was performed in all cases according to the WHO classification system. (journalmc.org)
  • Mucinous tubular and spindle cell carcinoma of the kidney is a rare and a distinctive renal tumor with a relatively indolent behavior. (journalmc.org)
  • Mucinous tubular and spindle cell carcinoma (MTSCC) is a rare variant of renal cell carcinoma, which has recently been described in case reports or small series [ 1 ]. (journalmc.org)
  • In gastric carcinoma cells, EBV is not integrated into the host genome but maintained as a type of plasmid called an episome. (spandidos-publications.com)
  • The uniformity of the numbers of terminal repeats (TRs) among EBV positive carcinoma cells reflects the clonal origin of a tumor and suggests that EBV is a causative virus for gastric carcinoma ( 6 ). (spandidos-publications.com)
  • No chromosome was found contrary to an extra chromosome 5 in the basal cell carcinoma. (globale-dermatologie.com)
  • Renal cell carcinoma (RCC) is the most common solid lesion within the kidney and accounts for ~90% of all kidney malignancies. (spandidos-publications.com)
  • After the operation, the pathological examination results suggested the following: Renal clear cell carcinoma, pT1aN0M0, the tumor sized ~4.0×4×3.5 cm 3 , with no lymph node cancer metastases or distant metastasis. (spandidos-publications.com)
  • Upon specific activation EBV may switch into a productive ("lytic") mode of infection in which viral progeny is definitely produced by the infected cell. (colinsbraincancer.com)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • One major obstacle to conducting the most clinically relevant prostate cancer (PCa) research has been the lack of cell lines that closely mimic human disease progression. (wikipedia.org)
  • Although the generation of AI cell lines has been quite successful as demonstrated in the "classic" cell lines DU145 and PC3, the behavior of these cells in bone does not fully mimic clinical human disease. (wikipedia.org)
  • MiR-146a-3p mimic and inhibitor were pre-transfected into RAW264.7 cells and the effects on M1 polarization induced by PM 2.5 were evaluated. (medsci.org)
  • These results show that, in addition to the well-known chromatin changes, nuclear organization undergoes other rearrangements during the apoptotic process. (nih.gov)
  • Additionally, RAD18 functions beyond DNA repair and may regulate many other key biological processes, such as chromatin strengthening, cell survival/death, stemness, and differentiation. (nature.com)
  • ChIP: Chromatin prepared from HUVEC cells. (abcam.com)
  • Keywords: T-cell activation Chromatin decondensation Calcium NFAT 1 Intro Following maturation in the thymus peripheral T-cells enter into a quiescent state characterized by a low metabolic profile rudimentary organelles and extremely condensed chromatin. (ecologicalsgardens.com)
  • Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. (ox.ac.uk)
  • DRAM2 acts as an oncogene in non-small cell lung cancer and suppresses the expression of p53. (cancerindex.org)
  • Here, we show that DRAM2 may act as an oncogenic regulator in non-small cell lung cancer (NSCLC). (cancerindex.org)
  • Analysis of transcription factor (TF) and ChIP- seq datasets identified overlap of 70 SNPs with TF binding regions of relevant lung cell lines (e.g. (cdc.gov)
  • in two different experiments, b95-8 virus-infected bjab cells revealed a gradually increasing number of ebna-positive cells. (liverpool.ac.uk)
  • in contrast, it has not so far been possible to convert ramos cells into an ebna-positive cell line. (liverpool.ac.uk)
  • With the goal of further improving cytokine-based immunotherapy, we examined the effects of a soybean peptide called lunasin that synergistically works with cytokines on natural killer (NK) cells. (weeksmd.com)
  • The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
  • Given the high frequency of DDX5 amplification in breast cancer, our results highlight DDX5 as a promising candidate for targeted therapy of breast tumors with DDX5 amplification, and indeed we show that DDX5 inhibition sensitizes a subset of breast cancer cells to trastuzumab. (aacrjournals.org)
  • Cells deprived of steroid hormones were exposed to increasing concentrations (1-100 μM) of pomegranate compounds in the presence of 0.1 nM dihydrotestosterone (DHT), and the inhibition of cell growth was measured by WST-1 colorimetric assay after a 4 day exposure. (nih.gov)
  • IL-6 was immunologically detected in basal keratinocytes, endothelial cells and in a number of mononucleated cells and fibroblasts in normal skin and sudoriparous ducts. (medicaljournals.se)
  • In the oral mucosa, melanocytes are observed in a ratio of about 1 melanocyte to 10 basal cells. (medscape.com)
  • Here, we demonstrate that the dNTPase activity of SAMHD1 is regulated by acetylation, which promotes cell cycle progression in cancer cells. (oncotarget.com)
  • In recent years, adult stem cell-based therapy was suggested as a potential approach to attenuate DEB progression. (biomedcentral.com)
  • They provide novel information on the mechanisms of cell-cycle progression and control, and have practical implications for oncology research. (edu.au)
  • 1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)
  • Finally, in vivo histopathological analysis on F. hepatica -parasitized liver tissue sections of sheep showed multifocal infiltration of inflammatory cells within liver parenchyma, and further fluorescence microscopy analyses confirmed NETs formation in vivo. (biomedcentral.com)
  • In addition, NK cells stimulated with lunasin plus cytokines displayed higher tumoricidal activity than those stimulated with cytokines alone using in vitro and in vivo tumor models. (weeksmd.com)
  • This is the first time identifying single-cell transcriptional landscape of HIV-1 RNA + cells at their in vivo state without ex vivo stimulations. (yale.edu)
  • In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)
  • During PhD, she developed the first HIV-1 full-length single-genome sequencing method that became the standard measurement of the size of the HIV-1 latent reservoir ( Cell 2013 ). (yale.edu)
  • High-affinity specific androgen and estrogen receptors are present in the cytosol and nuclear fractions. (wikipedia.org)
  • Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. (everestbiotech.com)
  • Na?ve T-cells express the intermediate affinity IL-2. (ecologicalsgardens.com)
  • The Igmmonoclonalprotein reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (ecceterra.fr)
  • production of interferon or interferon-like susbstances in eb3 cultures after inoculation with human serum containing australia antigen]. (liverpool.ac.uk)
  • As the first defense line, macrophage is one of the most important parts of innate immune system and is a cross-link between innate immunity and adaptive immunity. (medsci.org)
  • The immune system plays a key role in surveillance against cancer, but some tumour cells evolve to escape immune elimination [ 4 ]. (biomedcentral.com)
  • B cells The immune system consists of cellular components and molecular components that work together to destroy antigens. (msdmanuals.com)
  • Deoxyribonucleotide triphosphates (dNTPs) are the precursors of DNA synthesis, and their strict balance is critical for proper DNA replication and repair in cells [ 1 ]. (oncotarget.com)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • Understanding how DNA replication is regulated in human cells can provide insight into cancer development and may reveal vulnerabilities that can be exploited therapeutically. (aacrjournals.org)
  • However, the mechanism through which RAD18 influences triple-negative breast cancer (TNBC), especially the interaction between tumor cells and the tumor microenvironment, remains elusive. (nature.com)
  • We find that the DDX5 locus is frequently amplified in breast cancer and that breast cancer-derived cells with amplification of DDX5 are much more sensitive to its depletion than breast cancer cells and a breast epithelial cell line that lacks DDX5 amplification. (aacrjournals.org)
  • 1994) reproduced the human-derived LNCaP tumors in immunocompromised mice by co-injection of LNCaP cells with MS human bone fibroblasts. (wikipedia.org)
  • The development of teeth is initiated by epithelial-mesenchymal interactions, which form dental papilla cells and dental sac cells, subsequently forming pulp, dentin, cementum, periodontal ligament and proper alveolar bone, except enamel 1 - 4 . (researchsquare.com)
  • letters to the editor: bone marrow transplantation and leukemic transformation of transplanted cells]. (liverpool.ac.uk)
  • Histologically, lesions revealed true bone formation with multiple osteoblastic cells. (medicaljournals.se)
  • Primary Myelofibrosis Primary myelofibrosis (PMF) is a chronic myeloproliferative neoplasm characterized by bone marrow fibrosis, splenomegaly, and anemia with nucleated and teardrop-shaped red blood cells. (msdmanuals.com)
  • Leukocyte-platelet-rich plasma (L-PRP) is a bloodderived portion composed of high concentrations of platelets and white cells, and it has been applied as autogenous biomaterial in bone repair 13 . (bvsalud.org)
  • Further studies confirmed that p75NTR participates in the regulation of tooth development maybe by changing the activity of the key factor distal-less homeobox/msh homeobox (Dlx/Msx), and melanoma-associated antigen D1 (Mage-D1) seems to be play a role in the differentiation and mineralization of EMSCs 5 , 6 . (researchsquare.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
  • Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
  • To further select for AI-PCa cells, the C4 subline was co-injected with MS human fibroblasts into a castrated host. (wikipedia.org)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • A key mechanism of immune evasion deployed by tumour cells is to reduce neoantigen presentation through down-regulation of the antigen presentation machinery. (portlandpress.com)
  • 1999 Regulation of IL-2 signaling can be critically very important to clonotypic development mainly because those T-cells that TCR ligation hasn't occurred should be able to disregard the potent ramifications of this cytokine. (ecologicalsgardens.com)
  • To develop an AI-PCa cell model that more closely mimics clinical disease, LNCaP sublines have been generated to provide the most clinically relevant tissue culture tools to date. (wikipedia.org)
  • Dr. Ho's research program focuses on understanding HIV-1 persistence and HIV-1-induced immune dysfunction using single-genome and single-cell approaches on clinical samples. (yale.edu)
  • She is currently working on understanding HIV-1-induced immune dysfunction and clonal expansion dynamics of HIV-1-infected cells using single-cell multi-omic ECCITEseq on clinical samples ( Immunity 2022 ). (yale.edu)
  • clinical applications of cell surface markers. (liverpool.ac.uk)
  • The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
  • A luciferase reporter assay, in A549 cells, identified allele-dependent mRNA transcription. (cdc.gov)
  • They are adherent epithelial cells growing in aggregates and as single cells. (wikipedia.org)
  • Due to inherent toxicity, the DA phenotype is likely attributable to innate immune responses, oxidative stress, and epithelial cell barrier injury. (cdc.gov)
  • EB06619 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. (everestbiotech.com)
  • Approx 50kDa band observed in lysates of cell line HeLa and approx 55kDa in lysates of cell line Jurkat (calculated MW of 51.5kDa according to NP_057207.1). (everestbiotech.com)
  • Flow cytometric analysis of HeLa cells. (everestbiotech.com)
  • Methods: Cells from the MIA-PaCa-2, PANC-1, and HeLa cell lines were plated on gridded dishes and imaged using a multispectral fluorescence microscope. (edu.au)
  • Results: Across cancer cell lines accuracy ranged from 68.3% (MIA-PaCa-2) to 73.3% (HeLa) for distinguishing G1 from S and G2 + M, and 69.0% (MIA-PaCa-2) to 78.0% (PANC1) for distinguishing S from G2 + M. Unmixing the multispectral data showed that the autofluorophores NADH, FAD, and PPIX had significant differences between phases. (edu.au)
  • Multispectral analysis of endogenous cell autofluorescence has been shown to be sensitive to changes in cell status and could be applied to the discrimination of cell cycle without these steps.Methods: Cells from the MIA-PaCa-2, PANC-1, and HeLa cell lines were plated on gridded dishes and imaged using a multispectral fluorescence microscope. (edu.au)
  • The LNCaP line is hormonally responsive, shown by in vitro 5 alpha-dihydrotestosterone modulation of cell growth and acid phosphatase production. (wikipedia.org)
  • Both cell-derived microstructures and extracellular matrix components play critical roles in the preorganization and oriented deposition of calcium phosphate and serve as passive supports in dentine and enamel. (researchsquare.com)
  • PMN may display neutrophil extracellular traps (NETs), consisting of nuclear DNA, decorated with histones, enzymes, and antimicrobial peptides. (biomedcentral.com)
  • Extracellular vesicles (EVs) are a group of cell-derived membranous structures, which play an important role in intercellular communication and functional transduction. (dovepress.com)
  • For example, RAD18 plays a vital role in regulating the DNA stability of embryonic stem cells and cellular homeostasis in highly prolific cells [ 6 ]. (nature.com)
  • Let us underline nevertheless that such cytokeratin expression is found in embryonic cell lines and that the study only concerned 10 specimens. (globale-dermatologie.com)
  • We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)
  • LNCaP cells are androgen-sensitive human prostate adenocarcinoma cells derived from the left supraclavicular lymph node metastasis from a 50-year-old caucasian male in 1977. (wikipedia.org)
  • The key mechanism that regulates recruitment of leukocytes and progenitor stem cells to distal anatomical tissues affected with disease is chemotaxis, which depends on the signaling molecules, chemokines, and acts primarily as part of the host defense and repair mechanism. (biomedcentral.com)
  • LNCaP cells are a cell line of human cells commonly used in the field of oncology. (wikipedia.org)
  • LNCaP cells have a modal chromosome number of 76 to 91, indicative of a human male karyotype with several marker chromosomes. (wikipedia.org)
  • LNCaP cells also express Prostate Specific Antigen (PSA). (wikipedia.org)
  • To replicate the hallmark shift of PCa cells to AI, LNCaP host mice were castrated by way of midscrotal incision at approximately 8 weeks post injection. (wikipedia.org)
  • Karyotype comparisons indicate that LNCaP cells grown in intact hosts (M subline) have a modal chromosomal distribution number of 83, C4 and C5 sublines with 85, and the C4-2 subline with 83. (wikipedia.org)
  • To further verify that these cells were of human origin karyotype analysis determined that the parental LNCaP cells had 7 distinct marker chromosomes, with two copies of each. (wikipedia.org)
  • The M, C4, C5, and C4-2 sublines contained most of the marker chromosomes, with the M subline being most similar to the parental LNCaP cells. (wikipedia.org)
  • DHT treatment elicited markedly reduced growth in C4 and C5 cells and no growth in C4-2 cells when compared to the high rate of growth seen in LNCaP cells, suggesting reduced androgen sensitivity in C4 and C5 and AI in C4-2 cells. (wikipedia.org)
  • Lane 3: LNCaP cells. (novusbio.com)
  • In this study, we investigated the growth inhibitory, antiandrogenic, and pro-apoptotic effects of 13 pure compounds found in the pomegranate in androgen-dependent LNCaP human prostate cancer cells. (nih.gov)
  • In conclusion, punicic acid, the main constituent of pomegranate seed (70-80%), exhibited potent growth inhibitory activities in androgen-dependent LNCaP cells, which appear to be mediated by both antiandrogenic and pro-apoptotic mechanisms. (nih.gov)
  • Due to this fact, genetic engineering methods had been used to generate cytokines to boost tumor killing perform of CAR-T cells. (cellgenetherapy.com)
  • The initial NIOSH R01 grant award (2006-2012) identified candidate variants (snps) linked to Th2 cytokines, antioxidant enzymes, and HLA class I and II antigens. (cdc.gov)
  • The purpose of this study was to investigate the expression pattern of Mage-D1 in rat tooth germ development and its differential mineralization ability to ectomesenchymal stem cells (EMSCs), and to explore its potential mechanism. (researchsquare.com)
  • Ectomesenchymal stem cells (EMSCs) derived from the cranial neural crest are significant in tooth development and dental mineralization. (researchsquare.com)
  • The molecular mechanisms that govern directional migration of the adult stem cells, allowing their efficient and controlled homing to the skin affected with DEB, are poorly understood. (biomedcentral.com)
  • Systemic transplantation of an enriched CXCR2 population of mouse adipose-derived stem cells (mADSC) into DEB-affected mice demonstrated effective recruitment of cells to the blistering skin under the influence of blister-derived ligands and deposition of therapeutic type VII collagen. (biomedcentral.com)
  • Collectively, these studies demonstrate that recruitment of mADSC into DEB skin is tightly controlled by disease-site chemotactic activities and suggest a potential mechanism for effective application of therapeutic stem cells for DEB. (biomedcentral.com)
  • Can we wind back the clock in adult stem cells? (edu.au)
  • To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
  • The discovery of MHC-II on tumour cells has been less characterized. (portlandpress.com)
  • This review provides a comprehensive overview of physiologically conserved or novel mechanisms utilised by tumour cells to reduce MHC-I or MHC-II expression. (portlandpress.com)
  • The contribution of EBV to the etiology of PTLD is definitely inferred from the high proportion of EBV-positive pediatric PTLDs (70%) [3 10 which is much higher than that observed within the B-cell reservoir of latently infected healthy EBV service providers where only one in 1 0 to 100 0 peripheral B cells is definitely EBV-positive [11]. (colinsbraincancer.com)
  • The ISL IVR was found to be safe and well tolerated in the macaques with no observed mucosal cytokine changes or alterations in peripheral CD4 T-cell populations. (cdc.gov)
  • Exosomes are a type of membrane vesicles secreted by living cells. (dovepress.com)
  • abstract = "Background: Cell cycle analysis is important for cancer research. (edu.au)