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  • mammalian
  • Anson DS, Austen DEG and Brownlee GG (1985) Expression of human clotting factor IX from recombinant DNA clones in mammalian cells. (springer.com)
  • A major problem in generating stable transgenic cell lines in any mammalian cells, including hESC, is the silencing of the transgene by the epigenetic modification of the genomic integration site. (europa.eu)
  • Recent findings in different mammalian cell lines (including human cell lines) have shown that two pairs of insulator sequences flanking the transgene can substantially prevent the lost of its activity. (europa.eu)
  • Glis3 has been found to associate with the primary cilia, tiny hair-like sensory organelles extending from the surface of most mammalian cells. (murraystate.edu)
  • Packaging and proliferating lentivirus with RNA interference (RNAi) technique is a conserved biological response to double-stranded RNA (Hannon, 2002), RNAi is a sequence-specific process that regulates genetic functions and provides defense against virus at the post-transcriptional level in mammalian cells and animals (Song et al. (thefreedictionary.com)
  • bacteria
  • books.google.co.uk - In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. (google.co.uk)
  • porcine
  • Generation of a porcine alveolar macrophage cell line for the growth of porcine reproductive and respiratory syndrome virus. (semanticscholar.org)
  • Porcine reproductive and respiratory syndrome virus (PRRSV) has a marked tropism for cells of the monocyte-macrophage lineage and accordingly, replicates in fully differentiated alveolar macrophages in the natural host. (semanticscholar.org)
  • Despite the identification of several putative receptors for PRRSV on porcine alveolar macrophages (PAM), only CD163 was found to be able to make non-permissive cells susceptible to PRRSV, indicating a requirement for CD163 in productive infection. (semanticscholar.org)
  • tissues
  • When blood glucose levels are elevated, the pancreatic β cells produce insulin, which signals the cells of the peripheral tissues to take up circulating glucose. (murraystate.edu)
  • Both cultured cells and intact plants have served as experimental material, and a wide variety of tissues in various stages of development have been sampled. (plantcell.org)
  • artificial
  • First, two recombinant shRNA-expressing plasmids, PB-EN3D2B and PB-N3D2B, were constructed and the efficiency of the constructs for suppressing an artificial target was demonstrated in BHK-21 cells. (biomedcentral.com)
  • vacuoles
  • In addition, wortmannin caused the GFP-marked prevacuolar organelles to form small vacuoles, and VSR antibodies labeled these enlarged MVBs in transgenic BY-2 cells. (plantphysiol.org)
  • Anthocyanic vacuolar inclusions (AVI) in cell vacuoles of muscadine callus culture. (intechopen.com)
  • tobacco BY-2
  • Therefore, the seven AtVSRs are localized to MVBs in tobacco BY-2 cells, and wortmannin-induced vacuolation of PVCs is a general response in plants. (plantphysiol.org)
  • Generation
  • Faithful transmission of chromosomes during eukaryotic cell division requires sister chromatids to be paired from their generation in S phase until their separation in M phase. (embopress.org)
  • These data suggest that physiologic cells expressing IDO inhibit the generation of T cell responses in vivo. (jimmunol.org)
  • promoter
  • As expected, in HeLa cells the activity of the CMV promoter was rapidly silenced if the cells were transfected with non-insulated construct. (europa.eu)
  • transgene
  • This vector is a DNA vehicle, which transfers the DFR gene fragment (called transgene) into muscadine grape cells following Agrobacterium-mediated transformation. (intechopen.com)
  • inhibits
  • To explain these phenomena we hypothesized that proximity to cells expressing IDO inhibits T cell activation, possibly due to localized depletion of the essential amino acid tryptophan ( 7 , 12 ). (jimmunol.org)
  • 1987
  • Costanzi C and Gillespie D (1987) Fast blots: immobilization of DNA and RNA from cells. (springer.com)
  • PRRSV
  • Furthermore, the ability of pCD163-MARC cell line to propagate PRRSV was significantly increased as compared with MARC-145 cells. (hindawi.com)
  • This pCD163-MARC cell line will be a valuable tool for propagation and research of PRRSV. (hindawi.com)
  • fetal
  • Doubling times were lowest, as clonogenicity in soft agar was highest for Tu-2449, and to a lesser degree, Tu-2449 cells formed spheroids and showed migratory behaviour and invaded fetal rat brain aggregates. (uzh.ch)
  • production
  • Basic concepts for the development of biotechnological system for anthocyanins production based on grape cell suspension culture. (intechopen.com)
  • Research
  • It is clear that to derive maximum benefits from the use of transgenic plants for biotechnology and basic research, gene silencing must be understood, and methods to combat gene silencing must be devised. (plantcell.org)