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  • Neurons
  • Antibiotic & Antimycotic Their embryological origin with neuroblastic cells means they can easily differentiate into neuron-like cells even though they are not considered adult neurons. (wikipedia.org)
  • HeLa
  • To minimize the chances that HeLa or other cell lines will cause cross-contamination problems-and potentially give rise to spurious results-researchers are encouraged to follow "best practices" recommendations, such as those issued by the Global Biological Standards Institute. (genengnews.com)
  • Contaminated cell lines should never be used for research demanding the specific type of cell line they are assumed to be, and most of them should ideally be discarded or at least not used in research at all, except when the contaminant cells have acquired novel characteristics (e.g., by mutation or viral transfection, for example the HeLa derivate Det98) and thus constitute a novel lineage after all. (wikipedia.org)
  • It is worth noting that the widespread contamination with HeLa cells was initially recognized by Walter Nelson-Rees using simple Giemsa stain karyotyping under a light microscope. (wikipedia.org)
  • This technique works well in recognizing HeLa because these cells have distinctive chromosome aberrations. (wikipedia.org)
  • It is good practice to periodically check cell lines maintained under laboratory conditions (i.e., not placed in long-term storage) for contamination with HeLa or other common contaminants, to ensure that their quality and integrity is maintained. (wikipedia.org)
  • undergo
  • For the majority of isolated primary cells, they undergo the process of senescence and stop dividing after a certain number of population doublings while generally retaining their viability (described as the Hayflick limit). (wikipedia.org)
  • stem cell
  • Submit your event on Stem Cell Lines to be featured. (novusbio.com)
  • A stem cell line is characterized by a group of cells originating from a single parent cell. (novusbio.com)
  • Please visit our Neural Stem Cell Lines page here for more information on our NSCs and media/supplemental reagents. (novusbio.com)
  • A new study, published Thursday in the journal Cell Stem Cell , may illustrate part of the reason why. (latimes.com)
  • Stem cell scientists at UC San Diego and the Scripps Research Institute used a high-resolution molecular technique called "single nucleotide polymorphism" (SNP) analysis to study stem cell lines. (latimes.com)
  • He has since become a researcher in the field of bioethics, publishing over 100 articles, book chapters, and reviews on a wide range of topics, including organ transplantation, genetics, stem cell research, end of life care, patient communication, and research ethics. (wikipedia.org)
  • Hematopoietic stem cell transplantation is also used, especially for patients under 30 years of age with a related matched marrow donor. (wikipedia.org)
  • 2016
  • The notice's guidelines, which become effective January 16, 2016, were discussed September 28-29 by various stakeholders at the NIH Workshop on Reproducibility and Cell Culture Studies. (genengnews.com)
  • grown
  • the cells can be grown indefinitely in culture. (wikipedia.org)
  • Cells are grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum (FBS) in a humid, 5% CO2-enriched atmosphere at 28 °C. Under these conditions, cells passaged 1 in 4 doubled every 72 hours when fed with fresh culture medium at 3-day intervals. (wikipedia.org)
  • Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. (wikipedia.org)
  • Most cells require a surface or an artificial substrate (adherent or monolayer culture) whereas others can be grown free floating in culture medium (suspension culture). (wikipedia.org)
  • growth
  • Cells that are fairly density dependent for growth (NIH 3T3 cells, for ex. (bio.net)
  • The derived cell clones could be classified according to their growth properties in vivo into one (1) non-tumorigenic (growing as surface epithelia) and two tumorigenic groups, forming either (2) benign cysts or (3) well-differentiated squamous cell carcinomas. (springer.com)
  • Working in viruses of aquaculture we have a number of raggedy primary monocultures that we have been tiptoeing around in the hopes of getting a cell line for routine use in screening.Are there any methods involving growth enhancing chemicals or carcinogens that may help speed this process up? (bio.net)
  • PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor or dexamethasone. (wikipedia.org)
  • Treatment of PC12 cells with nerve growth factor creates cells with long processes known as neurite varicosities, which contain small amounts of vesicles. (wikipedia.org)
  • PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the aggregation of vesicles in the ends of the neurites. (wikipedia.org)
  • Because of slow growth rates and easy detachment of the original NCI-H295 strains, efforts were made to select a population of cells with better monolayer attachment and more rapid growth. (wikipedia.org)
  • however, only the white cells are capable of growth in culture. (wikipedia.org)
  • Alternatively, pieces of tissue can be placed in growth media, and the cells that grow out are available for culture. (wikipedia.org)
  • research
  • I'm trying to locate a rat neuroblastomal cell line for some research, but have not had much luck. (bio.net)
  • Hybridomas expressing various combinations of gamma delta T cell receptors available as research tools. (nationaljewish.org)
  • A common contributor to this significant waste of research resources is the widespread use of misidentified or contaminated cell lines, which are estimated to be the source of more than one-third of all cells used in research. (genengnews.com)
  • In April 2015, the Global Biological Standards Institute (GBSI) launched a multifaceted campaign to raise awareness of the importance of cell-line authentication in improving research reproducibility. (genengnews.com)
  • Documented authentic, contaminant-free cell lines should be used in research. (genengnews.com)
  • Research grants should include the costs to address cell-line authentication. (genengnews.com)
  • Tumor cell lines become more powerful research models and drug discovery tools when the genetic abnormalities that drive their phenotype are known. (atcc.org)
  • Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. (wikipedia.org)
  • Many cell lines that are widely used for biomedical research have been contaminated and overgrown by other, more aggressive cells. (wikipedia.org)
  • U937 cells are a model cell line used in biomedical research. (wikipedia.org)
  • For example, most if not all research on the endothelium ECV-304 or the megakaryocyte DAMI cell lines has in reality been conducted on bladder carcinoma and erythroleukemia cells, respectively. (wikipedia.org)
  • Thus, all research on endothelium- or megakaryocyte-specific functions utilizing these cell lines has turned out to be worthless, except as a warning example. (wikipedia.org)
  • It requires lengthy research to determine the precise point where cell lines have become contaminated. (wikipedia.org)
  • His research interests include metabolic network modelling, systems biology, tissue engineering and cell culture, the development of analysis procedures for genome-scale models, and the experimental verification of these models in Escherichia coli, Saccharomyces cerevisiae and other important organisms. (wikipedia.org)
  • Dame Amanda Gay Fisher DBE FRS FMedSci is a British cell biologist and Director of the Medical Research Council (MRC) London Institute of Medical Sciences at the Hammersmith Hospital campus of Imperial College London, where she is also a Professor leading the Institute of Clinical Sciences. (wikipedia.org)
  • Experimental Cell Research. (wikipedia.org)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)
  • genetic
  • They found that both embryonic stem (ES) cells and induced pluripotent stem (iPS) cells -- adult cells rewound to an embryonic state -- had more genetic abnormalities than other cell types. (latimes.com)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • tumour
  • Hi cell biologists, I am looking for tumour cell lines derived from childhood renal tumours such as Wilms' or the bone marrow metastasizing renal tumour of chilhood (BMRTC). (bio.net)
  • tissue
  • After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
  • Cells can be isolated from solid tissues by digesting the extracellular matrix using enzymes such as collagenase, trypsin, or pronase, before agitating the tissue to release the cells into suspension. (wikipedia.org)