• However, it remains unknown whether this heterogeneity may account for the stochasticity of cell fate decisions in stem cells. (nature.com)
  • Thus, clonal heterogeneity of gene expression level is not due to independent noise in the expression of individual genes, but reflects metastable states of a slowly fluctuating transcriptome that is distinct in individual cells and may govern the reversible, stochastic priming of multipotent progenitor cells in cell fate decision. (nature.com)
  • This causes the cell lineage and cell fate to be highly correlated. (wikipedia.org)
  • After mapping various sections of the C. elegans' cell lineage, Dr. Brenner and his associates were able to piece together the first complete and reproducible fate map of cell lineage. (wikipedia.org)
  • This high correlation between cell lineage and cell fate was thought to be determined by segregating factors within the dividing cells. (wikipedia.org)
  • Perhaps the most popular method of cell fate mapping in the genetic era is through site-specific recombination mediated by the Cre-Lox or FLP-FRT systems. (wikipedia.org)
  • With the system, researchers could investigate the function of their favorite gene in determining cell fate by designing a genetic model where within a cell one recombination event is designed for manipulating the gene of interest and the other recombination event is designed for activating a reporter gene. (wikipedia.org)
  • Marked lineages can be induced from specific cells within the organism by targeted laser irradiation, and the fate of the marked cells can be followed non-invasively. (nih.gov)
  • Bruce AW, Zernicka-Goetz M (2010) Developmental control of the early mammalian embryo: competition among heterogeneous cells that biases cell fate. (springer.com)
  • Cell-fate conversion of lymphoid-committed progenitors by instructive actions of cytokines," Nature , vol. 407, no. 6802, pp. 383-386, 2000. (hindawi.com)
  • The fate of RORγt + ILCs is determined by mouse age, and after birth, favors the generation of cells involved in intestinal homeostasis and defense. (sciencemag.org)
  • Multipotent hematopoietic cells gain access to the T cell developmental pathway and then confirm that choice of fate by "burning their bridges" to other pathways. (jimmunol.org)
  • Potential" is not the same as the default fate that a cell adopts in an undisturbed condition. (jimmunol.org)
  • Fate" is the intersection between the cell's potential and the permissive or nonpermissive circumstances in which a cell finds itself. (jimmunol.org)
  • At the point where potential, rather than environment, becomes limiting for fate, the cell is committed. (jimmunol.org)
  • Interestingly, one of the few cases where a "fate" assay approaches a "potential" assay is in the case of hematopoietic stem cells, where extremely long assay times are conventionally used, extremely large numbers of progeny are generated before the end point, and the intermediates include highly motile cells that can sample an unusually large number of in vivo environments before differentiating as scored at the assay end point. (jimmunol.org)
  • Chakrabarti S, Paek AL, Reyes J, Lasick KA, Lahav G, Michor F. Hidden heterogeneity and circadian-controlled cell fate inferred from single cell lineages. (harvard.edu)
  • However, unexpected observation of a developmental plasticity retained in mature T lymphocytes, in particular in CD4 + T-cell subsets, by recent studies is accelerating studies that focus on roles of each epigenetic pathway in cell fate decisions of T lymphocytes. (wiley.com)
  • We combined a genetic fate mapping approach with single cell expression analysis in a murine model of atherosclerosis. (fluidigm.com)
  • Tapetal cell fate, lineage and proliferation in the Arabidopsis anther. (jic.ac.uk)
  • Here we characterize the molecular and biological requirements for OCT4 plasticity induction in human skin derived fibroblasts (hFibs) that allows direct conversion of cell fate without iPSC formation. (wiley.com)
  • In response to changes in the extracellular environment, plastic OCT4-expressing hFibs further activate genes involved in hematopoietic as well as tripotent neural progenitor biology that allow cell fate conversion. (wiley.com)
  • Our study provides a working definition of hFib-induced plasticity using OCT4 and a deconvoluted system to elucidate the process of direct cell fate reprogramming. (wiley.com)
  • To track the cellular fate of stem and progenitor cells, which express C/EBPα, we developed a mouse model expressing Cre recombinase from the Cebpa promoter and a conditional EYFP allele. (bloodjournal.org)
  • Methodology and Principal Findings: To determine developmental pathways of TCR-transgenic thymocytes, we used Cre-LoxP-mediated fate mapping and show here that premature expression of a transgenic \(TCR\alpha\beta\) diverts some developing thymocytes to a developmental pathway which resembles that of gamma delta cells. (harvard.edu)
  • To determine the fate of aneuploid cells and the developmental potential of mosaic embryos, here we generate a mouse model of chromosome mosaicism. (sigmaaldrich.com)
  • Live-embryo imaging and single-cell tracking in chimeric embryos, containing aneuploid and euploid cells, reveal that the fate of aneuploid cells depends on lineage: aneuploid cells in the fetal lineage are eliminated by apoptosis, whereas those in the placental lineage show severe proliferative defects. (sigmaaldrich.com)
  • Whereas stem cells and their fates are known by unique genetic marker studies, the fate and function of these cells are best studied by their prospective isolation. (caltech.edu)
  • Mechanisms that control i NKT lineage fate decisions are still not well understood. (frontiersin.org)
  • Our data reveal that Foxo1 controls i NKT effector lineage fate decision by promoting i NKT-1 but suppressing i NKT-17 lineages. (frontiersin.org)
  • In addition, we will develop new strategies so that cells can write in their genomes a record of the molecular signals that control their fate. (caltech.edu)
  • The Discovery Center will allow us to answer fundamental questions about cell fate decision-making, which like real life involves many dimensions and is context dependent, by reading out the MEMOIR of those cells. (caltech.edu)
  • Our work demonstrates, for the first time, the dynamic role of Notch signaling in binary cell fate determination in Drosophila spermathecae and the role of ECs and ACs in secretory unit formation. (g3journal.org)
  • Mechanisms to Establish Polarity and Initiate Cell Fate Determination: Defining Cis-Acting Elements and Trans-Acting Factors in RNA Localization, K. Yaniv and J.K. Yisraeli. (bookdepository.com)
  • MEK1 was required to make Xenopus pluripotent cells competent to respond to all cell fate inducers tested. (xenbase.org)
  • With these cells, scientists will be able to better understand how these cells are differentiated by the process of turning on and off genes. (wikipedia.org)
  • The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. (pnas.org)
  • The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function. (pnas.org)
  • To further test the mb1-cre line, we bred it to several different lines bearing floxed genes, some of which are believed to be essential genes in all cell types. (pnas.org)
  • Our data confirmed that MDA-MB-435 cells indeed originated from breast epithelium, although they expressed melanocyte-specific genes, indicating that the MDA-MB-435 breast cancer cells underwent lineage infidelity during tumor progression. (aacrjournals.org)
  • Analyzing mutant genes within stem cell clones represents the critical test of whether and how particular genetic pathways control stem cell behavior. (stembook.org)
  • Further analysis at the single cell level showed that SM-derived CD68(+) cells expressed higher levels of inflammatory markers such as cyclooxygenase 2 (Ptgs2, p = 0.02), and vascular cell adhesion molecule (Vcam1, p = 0.05), as well as increased mRNA levels of genes related to matrix synthesis such as Col1a2 (p = 0.01) and Fn1 (p = 0.04), than non SM-derived CD68(+) cells. (fluidigm.com)
  • These results demonstrate that smooth muscle cells within atherosclerotic lesions can switch to a macrophage-like phenotype characterized by higher expression of inflammatory and synthetic markers genes that may further contribute to plaque progression. (fluidigm.com)
  • It will also allow us to deliver genes specifically into this cell type in vivo to test specifically molecules that have been implicated in cardiovascular development. (biologists.org)
  • 1993 ) Molecular cloning and characterization of mouse Tie and Tek receptor tyrosine kinase genes and their expression in hematopoietic stem cells. (biologists.org)
  • Human Fib-derived OCT4 plastic cells display elevated levels of developmentally related genes associated with multiple lineages, but not those associated with pluripotency. (wiley.com)
  • Transcriptome analysis and immunocytochemistry of EYFP+ cells revealed up-regulation of CPC markers including Mesp1, Mef2c, Irx4, Gata6, Tbx5, Tbx 20 accompanied by downregulation of fibroblast-specific genes such as Fsp1 and Thy1. (ahajournals.org)
  • Gene expression by EB was characterized by an up-regulation of a number of genes associated with trophoblast, ectoderm, endoderm, and mesoderm, and the production of hCG and progesterone confirmed that trophoblast-like cells were formed. (scirp.org)
  • article{8aba684a-86e7-470c-874f-9491a6b0585d, abstract = {Natural killer (NK) cells are cytotoxic lymphocytes and play a vital role in controlling viral infections and cancer. (lu.se)
  • Lineage Cell Therapeutics, Inc. operates as a clinical-stage biotechnology company developing new cellular therapies for degenerative retinal diseases, neurological conditions associated with demyelination, and aiding the body in detecting and combating cancer. (wsj.com)
  • ALAMEDA, Calif.--(BUSINESS WIRE)--BioTime, Inc. (NYSE American and TASE: BTX), a clinical-stage biotechnology company developing novel cell therapies for unmet medical needs, today announced it is launching a new corporate brand, including a change of its corporate name to Lineage Cell Therapeutics, Inc., effective August 12, 2019. (finanznachrichten.de)
  • The new website for Lineage Cell Therapeutics will be www.lineagecell.com. (finanznachrichten.de)
  • Our new brand defines us within our field: we control the lineage of pluripotent cells and transplant those differentiated cell types into patients as therapeutics. (finanznachrichten.de)
  • CARLSBAD, Calif.--( BUSINESS WIRE )-- Lineage Cell Therapeutics, Inc. (NYSE American and TASE: LCTX), a clinical-stage biotechnology company developing novel cell therapies for unmet medical needs, today announced that it has been awarded a new $670,621 Small Business Innovation Research (SBIR) grant to advance its Vision Restoration Program, the Company's proprietary and innovative program generating 3-dimensional human retinal tissue derived from pluripotent cells. (businesswire.com)
  • Grants for ophthalmology research from the NIH are highly competitive and we believe this new award and funding of our Vision Restoration Program serves as external validation of the potential of our approach to restore retinal tissue and provides continued proof of progress which builds upon prior SBIR grant awards received to date," stated Francois Binette, Ph.D., Sr. Vice President and Head of Global Development at Lineage Cell Therapeutics. (businesswire.com)
  • Lineage Cell Therapeutics is a clinical-stage biotechnology company developing novel cell therapies for unmet medical needs. (businesswire.com)
  • Lineage Cell Therapeutics received the CE Mark for its Renevia resorbable matrix, a Class III device used in treating facial lipoatrophy. (fdanews.com)
  • Cell lineage denotes the developmental history of a tissue or organ from the fertilized embryo. (wikipedia.org)
  • Cell transplantation and cell marking experiments have been carried out to determine the number of SMCs that convert when intermediate numbers of PMCs are present in the embryo. (nih.gov)
  • The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo. (harvard.edu)
  • By treating embryos with a spindle assembly checkpoint inhibitor during the four- to eight-cell division, we efficiently generate aneuploid cells, resulting in embryo death during peri-implantation development. (sigmaaldrich.com)
  • Finally, we show that mosaic embryos have full developmental potential, provided they contain sufficient euploid cells, a finding of significance for the assessment of embryo vitality in the clinic. (sigmaaldrich.com)
  • One of the fundamental questions in biology is to understand how a single-celled embryo gives rise to a complex animal with hundreds of different cell types organized into highly complex organs and tissues," says Lois, who will be one of the center's principal investigators. (caltech.edu)
  • We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines. (pubmedcentralcanada.ca)
  • In the current study, a high-throughput method that utilizes neutral somatic mutations accumulated in individual cells to reconstruct cell lineage trees was applied to hundreds of cells of human acute leukemia harvested from multiple patients at diagnosis and at relapse. (weizmann.ac.il)
  • g , β-galactosidase antibody staining on Tbx18 -lineage-traced tissue at E11.5. (nih.gov)
  • The majority of cells in an emerging lateral root primordium derive from the central file of pericycle founder cells while off-centre founder cells contribute only a minor proliferation of tissue near the base of the root. (nih.gov)
  • We find that the cell-intrinsic noise often causes reduction and oscillation of layer size whereas the cell-extrinsic noise increases the thickness, and sometimes, leads to uncontrollable growth of the tissue layer. (aimsciences.org)
  • The data presented provided evidence that retinal tissue produced in Lineage's laboratory from pluripotent cell lines was able to engraft tumor-free in a rat model for severe retinal degeneration and showed evidence of functional improvement. (businesswire.com)
  • In a separate presentation, Lineage demonstrated the ability to generate high quality retinal organoid tissue with a high number of maturing rod and cone photoreceptors from our highly characterized cGMP-grade pluripotent cell lines. (businesswire.com)
  • The progenitor cells identified in this study may be exploited to restore lymphatic function following cancer surgery, lymphedema, or tissue trauma. (diva-portal.org)
  • EATONTOWN, N.J., April 24, 2014 (GLOBE NEWSWIRE) -- American CryoStem Corporation (OTCQB:CRYO), a leading biotechnology developer and licensor of patented adipose tissue-based adult stem cell technologies, today announced that its ACS Laboratory division has completed its quarterly laboratory R&D program ahead of schedule, yielding new intellectual property and positive results for expanding and differentiating adipose-derived stem cells (ADSCs). (cnbc.com)
  • A biotechnology pioneer in the fields of Regenerative and Personalized Medicine, American CryoStem operates a state-of-the-art, FDA-registered, clinical laboratory dedicated to processing, bio-banking and development of cellular applications using autologous adipose (fat) tissue and adipose derived stem cells (ADSCs). (cnbc.com)
  • Finally, using basic knowledge of the target tissue and the guidelines discussed herein, we explain how raw lineage information can be converted into sound conclusions about the number, location, behavior and regulation of tissue stem cells. (stembook.org)
  • Although stem cells are critically important for tissue health and homeostasis, finding these cells and characterizing their cellular properties is difficult. (stembook.org)
  • Lineage analysis, a technique originally developed to study early embryos, represents by far the most powerful and reliable tool for identifying stem cells and for deciphering other aspects of tissue behavior. (stembook.org)
  • In the absence of extensive cell mixing, a thorough analysis of all the clonal types produced by a tissue allows the complexity of its component cell types and their movements to be determined. (stembook.org)
  • Finally, clonally analyzing stem cells is an essential prerequisite for discovering and characterizing the tissue niches that play an equal role with stem cells in ensuring a stable supply of replacement cells throughout the life of a tissue. (stembook.org)
  • In this hypothetical tissue, four stem cells (highlighted in dark blue at the beginning of the movie) are maintained in a niche (blue gradient highlighted in light blue at the beginning of the movie) produced by a population of non-dividing niche-generating cells (purple, highlighted in red at the beginning of the movie). (stembook.org)
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • In addition, generating multilayered mature retinal tissue may not be required for future cell-therapy strategies that are based on purified retinal specific cells. (molvis.org)
  • This unexpected autonomy of the tapetal 'lineage' is discussed in the context of tissue development in complex plant organs, where constancy in size, shape and cell number is crucial. (jic.ac.uk)
  • Recently, Lineage reported the first known finding of retinal tissue regeneration in a patient receiving OpRegen for the treatment of atrophic dry AMD. (benzinga.com)
  • Background: The binding of the T cell receptor (TCR) to major histocompatibility complex (MHC) molecules in the thymus determines fates of \(TCR\alpha\beta\) lymphocytes that subsequently home to secondary lymphoid tissue. (harvard.edu)
  • Most TCR transgenic mice express the rearranged \(TCR\alpha\beta\) prematurely at the double negative stage and abnormal TCRαβ populations of T cells that are not easily detected in non-transgenic mice have been found in secondary lymphoid tissue of TCR transgenic mice. (harvard.edu)
  • Likewise, DN \(TCR\alpha\beta\) cells in lymphoid tissue of female mice were not derived from DP thymocytes. (harvard.edu)
  • Analysing the results from19 layers gave the three dimensional reconstruction of a tumor lineage in a tissue volume. (epfl.ch)
  • The three dimensional reconstruction of a primary tumor lineage through a metastasis is therefore the reconstruction of the patters of metastasis growth in its host tissue. (epfl.ch)
  • Observing that most of the metastasis volume is not composed of tumor derived cells suggests that metastatic cells grow by diffusing through their host tissue and conditioning it to assume its abnormal morphology. (epfl.ch)
  • Adult stem cells, also termed as somatic stem cells, are undifferentiated cells, detected among differentiated cells in a tissue or an organ. (springer.com)
  • these cells would tend to be the most proliferative within a tissue. (rsc.org)
  • Thus, the molecular mechanisms elucidated in this work might provide the basic information for stem cell therapy for vascular disease, e.g. tissue engineering and endothelial repair in damaged vessels. (bmj.com)
  • Although their unique gene expression profiles eventually reverted to that of the median cells, revealing an attractor state, they lasted long enough to confer a greatly different proclivity for choosing either the erythroid or the myeloid lineage. (nature.com)
  • A CMP gives rise to the myeloid lineage, which comprises the rest of the leukocytes, the erythrocytes (red blood cells), and the megakaryocytes that produce platelets important in blood clotting. (genome.jp)
  • Such mechanisms do not however account for the change in lymphoid and myeloid lineage potential characteristic of HSC aging, which is believed to be central to the decline of immune competence and predisposition to myelogenous diseases in the elderly. (diva-portal.org)
  • Memory CD8 T cells can be divided into two subsets, central (T(CM)) and effector (T(EM)), but their lineage relationships and their ability to persist and confer protective immunity are not well understood. (nih.gov)
  • However, eGFP expression increases from CD4-/CD8- T-cells to single positive CD4+ and CD8+, and decreases in effector compared to naïve T-cells. (jimmunol.org)
  • These effector lineages identified thus far include IFN-γ-producing i NKT1, IL-4-producing i NKT2, IL-17A-producing i NKT17, i NKT10, Tfh-like i NKTfh, and Treg-like i NKT cells. (frontiersin.org)
  • Introduction: Joint fluid in patients with Lyme arthritis often contains high levels of CCL4 and CCL2, which are chemoattractants for monocytes and some T cells, and CXCL9 and CXCL10, which are chemoattractants for CD4+ and CD8+ T effector cells. (harvard.edu)
  • The percentage of T cells expressing CXCR3 or CCR5 was significantly greater in SFMC than PBMC, confirming that \(T_H1\) effector cells were recruited to inflamed joints. (harvard.edu)
  • Studies here finally demonstrate that individual HSCs sorted from adult bone marrow and transferred to lethally irradiated recipients clearly give rise to B-2, MZ B, and B-1b, but does not detectably reconstitute B-1a cells. (pnas.org)
  • These findings place B-2, MZ, and B-1b in a single adult developmental lineage and place B-1a in a separate lineage derived from HSCs that are rare or missing in adults. (pnas.org)
  • However, more detailed examination of the reconstituted B cells derived from HSCs taken at different times during development reveals differences in reconstitution efficiency for the four currently recognized murine B-cell subsets, [i.e. (pnas.org)
  • Importantly, however, because the Dorshkind studies are based on transfers of sorted HSC populations (roughly 1,000 sorted cells per recipient), they are not informative with respect to the potential of individual HSCs in the transferred population to give rise to each of the B-cell subsets (B-1a, B-1b, B-2, and MZ). (pnas.org)
  • This new assay method using a small number of cells would be of great advantage for clarifying which cells are truly P-HSCs. (labome.org)
  • The blood consist of many different specialized cells that all derive from rare hematopoietic stem cells (HSCs) located in the bone marrow in mice and humans. (diva-portal.org)
  • PURPOSE OF REVIEW: Hematopoietic stem cells (HSCs) possess two fundamental characteristics, the capacity for self-renewal and the sustained production of all blood cell lineages. (cnrs.fr)
  • Mechanistic drivers of hematopoietic stem cell (HSC) aging include DNA damage accumulation and induction of tumor suppressor pathways that combine to reduce the regenerative capacity of aged HSCs. (diva-portal.org)
  • In vivo, expression of Notch ligand with supportive cytokines, such as IL-7 and Kit ligand, gives the thymus its T cell inductive activity ( 3 ). (jimmunol.org)
  • For a typical uncommitted cell, the fates that its progeny actually adopt in vivo may be only a slice of the uncommitted cell's full potential, because environmental conditions are limiting. (jimmunol.org)
  • In this same cohort, we also intend to study the in vivo lifespan and phenotype of genetically modified NK cells and explore the efficacy of NK cells in patients with B-lineage ALL. (clinicaltrials.gov)
  • An in vivo model for cell lineage in pancreas of adult rat. (biomedsearch.com)
  • For example, major challenges currently faced within regenerative medicine include the assessment of purity of stem cells or stem cell-derived populations, the former to confirm faithful cellular reprogramming and the latter to eliminate the risk posed by introduction of undifferentiated stem cells in vivo. (pubmedcentralcanada.ca)
  • Using mouse skin as a model, Ge and colleagues utilized the assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) coupled with RNA and chromatin immunoprecipitation sequencing to compare in vivo chromatin states in homeostatic, wound-induced, and squamous cell carcinoma (SCC) stem cells. (aacrjournals.org)
  • During early embryogenesis, cells must exit pluripotency and commit to multiple lineages in all germ-layers. (xenbase.org)
  • The development of cells within a multi-cellular organism has similarities to the development of populations, and hence is amenable to study using concepts and tools of population genetics. (weizmann.ac.il)
  • Yet, cell populations possess unique features that are absent or rare in organism populations (e.g. the presence of stem cells and a small number of generations since the zygote). (weizmann.ac.il)
  • In all assays, sialoadhesin exhibited specific, differential binding to various murine cell populations of hemopoietic origin. (jci.org)
  • In contrast, B-1a cells are relatively well reconstituted by transfers of HSC populations sorted from "neonatal" BM (2.5 wk of age), although the sorted cells still predominantly reconstitute B-2 and B-1b ( 5 ). (pnas.org)
  • Normally, developmental potentials of two populations are compared by testing cells under uniform conditions that are clearly permissive for the fates being assayed. (jimmunol.org)
  • We have discovered new neutrophil-lineage cell populations based on this protocol. (fluidigm.com)
  • This protocol of fresh whole BM preparation may be used for 1), CyTOF analysis to discover unidentified cell populations from whole BM, 2), investigating whole BM defects for patients with blood disorders such as leukemia, 3), assisting optimization of fluorescence-activated flow cytometry protocols that utilize fresh whole BM. (fluidigm.com)
  • The understanding of these processes is largely facilitated by isolation of intermediate populations of cells at defined stages of development. (diva-portal.org)
  • I am isolating some specific sub-populations from breast cancer cell lines. (protocol-online.org)
  • Heteroplasmic variants can distinguish clonal cell populations within one individual. (elifesciences.org)
  • Also, early lineage cells were selectively depleted in T cell populations from pediatric patients treated with conventional chemotherapy. (chop.edu)
  • The results from these studies demonstrated that early lineage cells are essential for T cell fitness for expansion and enrichment of these populations-either by timing of T cell collection (prior to chemotherapy) or culture method-can increase the number of patients with B cell malignancies who may be eligible for treatment with engineered T cell immunotherapies. (chop.edu)
  • Stochastic gene expression in a single cell. (nature.com)
  • Gene regulation at the single-cell level. (nature.com)
  • QIAGEN provides a broad range of assay technologies for cell lineage research that enable analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation. (qiagen.com)
  • By utilizing the Cre-Lox or FLP-FRT recombination systems, a reporter gene (usually encoding a fluorescent protein) is activated and permanently labels the cell of interest and its offspring cells, thus the name cell lineage tracing. (wikipedia.org)
  • The Stem Cell Lineage Database could help compare stem cell information around the world and eliminate taking a global gene expression profiling approach, which although sufficient, is not as effective as it could be. (wikipedia.org)
  • Rapid intraclonal switch of lineage dominance in congenital leukaemia with a MLL gene rearrangement," Leukemia , vol. 9, no. 12, pp. 2023-2026, 1995. (hindawi.com)
  • The mb1 gene encodes the Ig-α signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. (pnas.org)
  • We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. (pnas.org)
  • We asked whether expression of the cre recombinase from the murine mb-1 locus would provide an even more efficient model for studying gene function specifically in B cell precursors. (pnas.org)
  • The mb1 gene encodes the Ig-α signaling subunit of the B cell antigen receptor ( 8 , 9 ). (pnas.org)
  • Deletion of the SRp20 gene in the mouse germ line blocks embryonic development at the morula-to-blastocyst transition ( 12 ), but its role in B cell development is not known. (pnas.org)
  • We have previously shown that both mature and UCA gp41 MPER bnAb heavy- (HC) and light-chain (LC) gene-rearranged (V H DJ H /V L J L ) KI mice have severe bone marrow (BM) deletion, and the few remaining B cells in the periphery are anergic, resulting in massive reduction in BM precursor frequency of MPER bnAbs 16 . (nature.com)
  • In Cell this week: gene editing-based strategy to screen for immune system regulators, ancient plague patterns, and more. (genomeweb.com)
  • Epigenetics has often been discussed in the context of the maintenance of cell identity because of the heritable nature of gene expression status. (wiley.com)
  • Sometimes researchers change the DNA (genetic material in cells) of donated T cells (white blood cells that support the immune system) using a process called 'gene transfer. (clinicaltrials.gov)
  • Gene transfer involves drawing blood from the patient, and then separating out the T-cells using a machine. (clinicaltrials.gov)
  • Researchers then perform a gene transfer to change the T-cells' DNA, and then inject the changed T-cells into the body of the patient. (clinicaltrials.gov)
  • The goal of this clinical research study is to learn if an investigational type of gene transfer can be given reliably and safely in patients with advanced B-cell lymphoma. (clinicaltrials.gov)
  • The gene transfer involves drawing blood, separating out T cells (white blood cells that fight infection and disease), changing the T cells' DNA (genetic material) in a specific way, and returning the changed T cells back to the body. (clinicaltrials.gov)
  • T Cell Infusion (Gene Transfer) by vein over 15-30 minutes sometime between Day +2 through Day +7. (clinicaltrials.gov)
  • 1992 ) tek , a novel tyrosine kinase gene located on mousechromosome 4, is expressed in endothelial cells and their presumptive precursors. (biologists.org)
  • 1994 ) Endothelial-cell-specific regulation of von Willebrand factor gene expression. (biologists.org)
  • Considerable knowledge of the ontogeny of the endocrine pancreas has been gained in recent years, mainly through the use of two complementary genetic approaches in transgenic mice: gene inactivation or over-expression (to assess gene function) and genetic labeling of precursor cells (to determine cell lineages). (springer.com)
  • Our goals herein were to determine if BG02 cells form trophoblast-like cells (a) in the presence of BMP4-plus-basic fibroblast growth factor (FGF-2) and (b) upon EB formation, and (c) whether the BMP4 antagonist noggin elicits direct effects on gene expression and hormone production in the cells. (scirp.org)
  • Last year, Elowitz and Cai collaborated on a gene-editing technique called MEMOIR , which allows cells to record information in their own genomes, in a format that can be read out by microscopic imaging. (caltech.edu)
  • Consistently, when Ventx2 lacked a functional PEST-destruction motif, it was stabilized, displayed symmetric distribution during cell division and could efficiently maintain pluripotency gene expression over time. (xenbase.org)
  • We analyze acquired somatic mutations to reconstruct lineage trees of hundreds of oocytes and other cells, sampled from mismatch-repair deficient mice at various ages. (weizmann.ac.il)
  • The funding from the Allen Discovery Center will allow us to develop new methods with which to reconstruct lineage trees over many generations. (caltech.edu)
  • Our lab pioneered the concept, the mathematical foundations, as well as the implementation of utilizing somatic mutations naturally acquired by individual cells, to reconstruct cell lineage trees among cells of multi-cellular organisms and applied it to various questions of biological and medical importance. (weizmann.ac.il)
  • In particular, the reconstruction of a cell lineage tree, capturing the cell division history of organism cells, can be attempted by applying algorithms and techniques of population genetics to somatic mutations accumulated during cell division. (weizmann.ac.il)
  • We applied a method for reconstructing cell lineage trees from somatic mutations to MSCs and myogenic and non-myogenic cells from individual myofiber that were cultured at clonal density. (weizmann.ac.il)
  • We show that somatic mutations in mitochondrial DNA can reconstruct cell lineage relationships at single cell resolution with high sensitivity and specificity. (elifesciences.org)
  • Human cornea derived from normal or squamous cell carcinoma (SCC) samples were acquired, cryosectioned (10 µm), floated onto BaF 2 windows and interrogated using synchrotron -based radiation ( SRS ) Fourier-transform IR ( FTIR ) microspectroscopy . (rsc.org)
  • They later received the 2002 Nobel prize for their work in genetic regulation of organ development and programmed cell death. (wikipedia.org)
  • BioTime's programs are based on its proprietary cell-based therapy platform and associated development and manufacturing capabilities. (finanznachrichten.de)
  • and (iii) VAC2, an allogeneic cancer immunotherapy of antigen-presenting dendritic cells currently in Phase I development for the treatment of non-small cell lung cancer. (finanznachrichten.de)
  • In the B cell system there are several transgenic mouse lines available that express cre in defined stages of B lymphocyte development. (pnas.org)
  • A reduction or loss of Dnmt1 activity has a drastic effect on cell function and, depending on the system, can lead to inhibition of DNA replication ( 14 ), T cell lymphoma ( 15 ), alterations in T cell development ( 16 ), and embryonic lethality ( 17 ). (pnas.org)
  • The results presented here show that SRp20 and Dnmt1 are essential for B cell development and/or survival and that cre recombinase activity in the mb1-cre line is efficient and primarily restricted to the B lineage. (pnas.org)
  • Precursors undertaking T cell development shed their access to other pathways in a sequential process that begins before entry into the thymus and continues through many cell cycles afterward. (jimmunol.org)
  • Epicardial derivatives, including vascular smooth muscle cells and cardiac fibroblasts, are crucial for proper development of the coronary vasculature and cardiac fibrous matrix, both of which support myocardial integrity and function in the normal heart. (mdpi.com)
  • Yutzey, K.E. Transcriptional Control of Cell Lineage Development in Epicardium-Derived Cells. (mdpi.com)
  • Indeed, crucial roles of the epigenetic machinery in establishment and maintenance of particular lineages during early development have been well documented. (wiley.com)
  • NEW YORK (GenomeWeb) - Independent research teams have come up with single-cell sequencing methods for following organismal development and teasing apart the targets of neurons passing along messages, respectively, in model organisms. (genomeweb.com)
  • Derived from classical approaches in cell lineage tracing during development, we review modern cell lineage methods and describe how they are used to identify stem cells and their niches. (stembook.org)
  • Vascular endothelial cells play essential roles in the function and development of the cardiovascular system. (biologists.org)
  • 1925 ) The development of adventitial (Rouget) cells on the blood capillaries of amphibian larvae. (biologists.org)
  • Unusually for plants, tapetal cells are specified very early in development, and are subsequently stimulated to proliferate by a receptor-like kinase (RLK) complex that includes EMS1. (jic.ac.uk)
  • 3 Additional evidence against a strict distinction between lymphoid and MEGM lineage development comes from studies showing that thymic progenitor cells maintained myeloid (GM) potential, indicating the existence of a common T-cell/myeloid progenitor in the thymus. (bloodjournal.org)
  • Development of germ cells was identified in three independent non-seminomas, including two pure yolk sac tumours and one mixed tumour composed of yolk sac tumour and immature teratoma. (ovid.com)
  • The first step in the development of lineage tracing methods using somatic mtDNA mutations as a marker, as a marker, is a quantitative, fast and high throughput method of detection. (epfl.ch)
  • In this report, we demonstrate that deletion of Foxo1 causes severe decreases of i NKT cell total numbers due to impairment of late but not early i NKT cell development. (frontiersin.org)
  • The iVα14 TCR signaling via multiple pathways such as RasGRP1-Ras-Erk1/2 pathway, the PKCθ-NFκB pathway, and mTOR is crucial for i NKT cell development in the thymus ( 2 , 5 - 12 ). (frontiersin.org)
  • The goal is for each cell to be able to tell us its own individual history, including its lineage and the specific molecular events it has experienced during development," says Elowitz. (caltech.edu)
  • The potential to generate an array of differentiated cell types also raises the opportunity to establish new models of early mammalian development ( Rossant, 2008 ). (pubmedcentralcanada.ca)
  • The aim of this thesis has been to identify the point of no return in B-cell development in order to allow for a better understanding of lineage restriction events in early lymphopoesis. (diva-portal.org)
  • This volume brings together current information on the localization and roles of RNAs in cell-lineage determination and subsequent patterning in embryonic development. (bookdepository.com)
  • CD19 is a B cell surface protein expressed throughout B cell development. (protocol-online.org)
  • Resolving lineage relationships between cells is necessary to understand the fundamental mechanisms underlying normal development and the progression of disease. (elifesciences.org)
  • Development of a screen using inner ear sensory hair cell-like cells made by direct lineage reprogramming, for discovering drugs to ameliorate hearing loss during cancer chemotherapy. (ca.gov)
  • Sialoadhesin is a macrophage-restricted, sialic acid-dependent receptor of 185 kD that binds to the oligosaccharide sequence NeuAc alpha 2,3Gal on cell surface glycoconjugates. (jci.org)
  • In naive CD4bs, unmutated common ancestor knock-in mice Env + B cell clones develop anergy and partial deletion at the transitional to mature B cell stage, but become Env − upon receptor editing. (nature.com)
  • LTi cells express the nuclear hormone receptor RORγt, which also directs the expression of the proinflammatory cytokine interleukin-17 in T cells. (sciencemag.org)
  • The recognition of ligand by the T cell antigen receptor (TCR) and the activation of intracellular signaling pathways have led to the coining of such terms as "positive selection," "negative selection," "serial triggering," "instructive process," and "kinetic signaling. (sciencemag.org)
  • Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. (uniprot.org)
  • Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. (labome.org)
  • We describe here the pivotal role of the LRR receptor kinase EXCESS MICROSPOROCYTES 1 (EMS1) in forming the monolayer of tapetal nurse cells in Arabidopsis. (jic.ac.uk)
  • Then, the expanded NK cells are transduced with a signaling receptor that binds to CD19 (anti-CD19-BB-zeta). (clinicaltrials.gov)
  • First, inferring the reverted unmutated ancestor of a mutated antibody gives an approximation of the naïve B cell receptor (BCR) from which the antibody origi- nated. (cavd.org)
  • Oligoclonal expansion of T-cell receptor (TCR) Vβ subfamilies and interferon gamma (IFN-γ) can be detected in peripheral blood mononuclear cells of these patients. (bloodjournal.org)
  • CXCR3, the receptor for CXCL9 and CXCL10, and CCR5, the receptor for CCL4, were assessed on T cells from PBMC and SFMC. (harvard.edu)
  • We found that most peripheral T cells with the HY-TCR in male mice have bypassed the RORγt-positive \(CD4^{+}8^{+}\) (double positive, DP) stage to accumulate either as \(CD4^{-}8^{-}\) (double negative, DN) or as \(CD8\alpha^{+}\) T cells in lymph nodes or gut epithelium. (harvard.edu)
  • Some organisms such as C. elegans have a predetermined pattern of cell progeny and the adult male will always consist of 1031 cells, this is because cell division in C. elegans is genetically determined and known as eutely. (wikipedia.org)
  • F. Doetsch , A niche for adult neural stem cells. (aimsciences.org)
  • The hematopoietic stem cell (HSC) derived from adult bone marrow (BM) is commonly thought to have multilineage potential, meaning that the HSC is considered capable of reconstituting all lymphoid, myeloid, and erythroid lineages of the immune system ( 1 , 2 ). (pnas.org)
  • Transferring adult BM into lethally irradiated recipients readily reconstitutes B-2 and MZ, which represent the majority of the B cells in spleen and other lymphoid organs but only poorly reconstitute B-1 cells in the same recipients. (pnas.org)
  • From one stem cell to many differentiated body cells: Scientists from the MDC in Berlin, along with collaborating researchers in Munich, have published a comprehensive lineage tree of a whole adult animal in the journal Science . (eurekalert.org)
  • By combining sophisticated single-cell RNA technology with nucleic acid sequencing and computational methods, the scientists were able to establish a detailed cell atlas of an entire complex adult animal, the Schmidtea mediterranea flatworm, and reconstruct the lineage tree of all identified cells. (eurekalert.org)
  • We have challenged this understanding by defining an adult pulmonary mesenchymal progenitor cell (MPC) that regulates both microvascular function and angiogenesis. (jci.org)
  • Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. (harvard.edu)
  • Wagers AJ, Weissman IL (2004) Plast Adult Stem Cells. (springer.com)
  • However, adult cardiac fibroblasts infected with all 22 factors yielded some EYFP expressing cells only after doxycycline treatment. (ahajournals.org)
  • The adult stem cell (SC) microenvironment is important in maintaining their SC state. (arvojournals.org)
  • Other organisms had stereotyped patterns of cell division and produced sublineages which were the progeny of particular precursor cells. (wikipedia.org)
  • We report the first vascular endothelial cell lineage-specific (including angioblastic precursor cells) 1.2 kb promoter in transgenic mice. (biologists.org)
  • Similarly, immunization of rhesus macaques with Env immunogens has initiated bnAb-like lineages that have been controlled either by deletion or affinity reversion (maturation off-target) due to selection of non-bnAb HCDR3 regions 19 . (nature.com)
  • CH103, a prototype of the HCDR3-binder class of CD4-binding site bnAbs, is one of the only two bnAb lineages whose complete virus-Ab co-evolution pathway has been comprehensively characterized 6 , and whose co-evolved Env maturation pathway, from which sequential immunogens have been derived for this study, can now also be investigated in SHIV CH505-infected non-human primates 20 . (nature.com)
  • We discovered that in the reconstructed lineage trees oocytes cluster distinctly from cells of bone marrow origin, show no lineage barrier between ovaries and increase in depth (number of cell divisions since the zygote) with mouse age, an increase accelerated after unilateral ovariectomy. (weizmann.ac.il)
  • Single-cell analyses confirmed that sialoadhesin selectively bound myeloid cells in complex cell mixtures obtained from the bone marrow and blood. (jci.org)
  • Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow," Blood , vol. 105, no. 11, pp. 4407-4415, 2005. (hindawi.com)
  • It is strongly expressed in the B cell lineage beginning at the very early pro-B cell stage in the bone marrow and continues to be expressed in all later stages except plasma cells ( 10 ). (pnas.org)
  • Furthermore, specific ablation of Nfil3 in either immature NK cells in the bone marrow or mature peripheral NK cells had no observable effect on NK cell lineage maintenance or homeostasis. (rupress.org)
  • In these mice, we did not detect infiltration of T cells in bone marrow (BM), and the existing T cells seemed to be hyporesponsive. (bloodjournal.org)
  • In combination with the autoMACS® Pro Separator, cell isolations can be automated allowing standardized procedures with minimal hands-on time for developing routine analyses. (miltenyibiotec.com)
  • Western blot analyses showed that proteins were up-regulated in the BMP2/4-treated C3H10T1/2 cells. (mcponline.org)
  • Albelda, S. M., Muller, W. A., Buck, C. A., and Newman, P. J. (1991) Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule. (springer.com)
  • The scientists also discovered several new cell types located in the parenchyma of the animals that had been previously overlooked in molecular studies and that play an important role in the regeneration of flatworms. (eurekalert.org)
  • Each alternative potential has a different underlying molecular basis that is neutralized and then permanently silenced through different mechanisms in early T cell precursors. (jimmunol.org)
  • This is a powerful molecular tool that will enable us to identify factors and cellular signals essential for the establishment of vascular endothelial cell lineage. (biologists.org)
  • In this review, we investigated UVB apoptotic action in various cell models by using ultrastructural, molecular and cytofluorimetric techniques. (mdpi.com)
  • With this kind of information, we will be able to understand the molecular signals that determine, for example, that a single stem cell will give rise to two daughter cells, with one becoming a neuron that processes visual information while the other processes auditory information. (caltech.edu)
  • The absence of immortal strand mechanism in crypts stem cells was validated by the age-dependent accumulation of microsatellite mutations. (weizmann.ac.il)
  • Inherited mitochondrial mutations have been a successful tool in the understanding of human lineage and migrations throughout history. (epfl.ch)
  • Lineage tracing in human tumors was achieved by scanning the mitochondrial DNA for mutations in samples extracted from macro-anatomical samples taken across surgically removed and freshly frozen tumor pieces. (epfl.ch)
  • The first attempt consisted in taking 286 samples across a slice of a Leydig cell tumor, in which two separate mutations were identified at a high enough fraction to justify further investigation. (epfl.ch)
  • Both mutations strongly correlated suggesting a single lineage. (epfl.ch)
  • ETS2 was sufficient to induce lineage plasticity, and sustained ETS2 activity, which occurs in the setting of constitutive MAPK signaling caused by HRAS mutations in SCC, maintained lineage plasticity and commissioning of tumor-specific enhancers. (aacrjournals.org)
  • EMBLEM reveals cell lineage from mtDNA mutations. (elifesciences.org)
  • EMBLEM identifies heteroplasmic mtDNA mutations in single cells, groups mutations into diagnostic sets, and infers cell lineage based on mtDNA variants, and overlays clonotype information on epigenomic profile of the same cells (right). (elifesciences.org)
  • E ) VAF of mtDNA mutations in single cell ATAC-seq data of human B cells. (elifesciences.org)
  • Variability and memory of protein levels in human cells. (nature.com)
  • Constitutive expression of a green fluorescent membrane protein was used to provide a precise outline of all surrounding cells. (nih.gov)
  • In this study, direct evidence for this interaction was obtained in cell-cell binding assays using both native and recombinant forms of the protein. (jci.org)
  • The ligand of the TCR for the majority of T cells (called αβ T cells) is another membrane protein found on the surface of antigen-presenting cells (APCs). (sciencemag.org)
  • However, like the generation of a 3D protein structure from its diffraction patterns, constructing an accurate picture of a tissue's dynamics from its lineage patterns is complex, non-intuitive, and fraught with potential problems. (stembook.org)
  • Proteins showing differential expression were isolated and identified using MALDI-MS and MALDI-MS/MS. B , enlarged images of differentially expressed protein spots between untreated and BMP2/4-treated C3H10T1/2 cells. (mcponline.org)
  • Strikingly, MEK1 appeared to control the asymmetric inheritance of Ventx2 protein following cell division. (xenbase.org)
  • By having this information openly available to the public, it gives researchers a place to meet and share information that could possibly enhance the experimental success of stem cell research as well as provide techniques to influence stem cells down specific pathways. (wikipedia.org)
  • The conversion of these cells to the skeletogenic phenotype is accompanied by their de novo expression of cell surface determinants normally unique to PMCs, as shown by binding of wheat germ agglutinin and a PMC-specific monoclonal antibody. (nih.gov)
  • NK cells have historically been considered components of the innate immune system, recognizing virally infected and tumor cells through germline-encoded receptors, and rapidly eliminating these targets through the secretion of lytic granules. (rupress.org)
  • Ultraviolet B (UVB) radiation acts as a strong apoptotic trigger in many cell types, in tumor and normal cells. (mdpi.com)
  • Under the assumption that a metastasis is seeded by a single tumor cell, the presence of the same mtDNA mutation in both primary tumor and metastasis guarantees that only cells carrying the mutation are tumor-derived. (epfl.ch)
  • In addition to providing insight into how tumor-associated stem cells lock in what is typically a transient wound-induced stem cell state in the skin, these findings suggest that drivers of sustained lineage plasticity represent potential therapeutic targets. (aacrjournals.org)
  • Mature CD4 + and CD8 + T cells derive from a common precursor in the thymus, a double-positive (DP) thymocyte, which has both co-receptors. (sciencemag.org)
  • i NKT cells are traditionally defined into four developmental stages in the thymus: stage 0 (CD24 + CD44 − NK1.1 − ), stage 1 (CD24 − CD44 − NK1.1 − ), stage 2 (CD24 − CD44 + NK1.1 − ), and stage 3 (CD24 − CD44 + NK1.1 + ) ( 13 , 14 ). (frontiersin.org)
  • A three dimensional reconstruction of a cell lineage was then established for 2 more tumors, and 3 metastasis. (epfl.ch)
  • These findings suggest that TTF1 expression in SCLC is a cell lineage-specific phenomenon that involves the developing neural cell-specific homeoprotein BRN2. (sigmaaldrich.com)